[BiO BB] Problem with BLAST

[Joe Landman]

Did you mean to translate your nucleotide sequence into a protein 
sequence?  You would need to use either blastx to do the translation, or 
one of the tblast series (depending upon what you were searching against).

Balaji wrote:
> Hi everyone,
>    I have a protein that comprises only of Alanine(A), Glycine(G),
> Cysteine(C) and Threonine(T). When i try to use NCBI BLAST with this
> protein, the blast program returns an error saying that the entered
> sequence is a nucleotide (as it evidently has only A's, T's, C's and
> G's) and not a protein sequence.
>    Is there anyway i work around the problem? I'll be grateful for any help
> Thanks in advance
> Balaji


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Joseph Landman, Ph.D
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