[BiO BB] Will this implementation of the lac operon work?

[Bryan Bishop]

Hi all,

I am designing a regulatory circuit and have been scratching my head 
over how to include enhancers, promoters and TATA boxes, etc. I have 
decided to make some progress by randomly guessing and getting feedback 
from the community. Here's my work:

http://heybryan.org/genetic-circuits.html

Basically I've taken the sequences of BBa_I14032, BBa_R0011, and 
BBa_B0034, attached the peptide I want to express, and have at it. It 
is my understanding, then, that given a lactose-full environment, my 
repressible circuit should express the peptide, and if I wanted to add 
a second circuit I could express a repressor that would bond to prevent 
RNA polymerase II from manufacturing my peptide as frequently. Correct?

The documentation is rather sparse, so once I get my head around this 
I'll be sure to go back and fill in the gaps in the documents.

Thanks,
- Bryan
(I also sent this over to OWW to see what they have to say.)
________________________________________
Bryan Bishop
http://heybryan.org/