MPI: --------stack traceback------- [blastall] ERROR: lcl|tmpseq_0: BlastKarlinBlkGappedCalc: â is not a supported Try running your query using normal blastall. It is possble that there was a communication error somewhere, from looking at the error report. The other possibility I see is that [mpiBLAST] Shared=/home/kalyani/toolkit/ncbi Local=/home/kalyani/toolkit/ncbi You want to seperate the shared and local directories (assuming that /home/kalyani is shared NFS). You want the local directory to be local to the compute node. It is quite possible/likely that you are overwriting some critical aspects of your data since the two are sitting atop each other. You might consider using /tmp as a local directory, though I usually prefer using local disk space outside system /tmp. kalyani wrote: > Hi all! > Iam running mpiblast with the following command. > mpirun -np 4 ~/bin/mpiblast -p blastp -d pir.fasta -i > /home/kalyani/hmmer-2.3.2/src/seq1 -o mpiblastoutput.txt > > Iam getting the following error. Can any one of you help me in finding out > the > error. Iam attaching the .ncbirc file > Thanks in Advance > Kalyani -- Joseph Landman, Ph.D Founder and CEO Scalable Informatics LLC, email: landman at scalableinformatics.com web : http://www.scalableinformatics.com phone: +1 734 786 8423 fax : +1 734 786 8452 cell : +1 734 612 4615