Great response for Sandy. I am in a similar position with a new cluster in our lab. I am still very early in the learning curve and your comments for Sandy are very helpful. We have and all vs all Blast job that we would like to run. I am trying to get a handle on how best to run this. I am wondering if you have any comments regarding all vs all tools. I am aware of blastn but was considering maybe Blat instead. We are mainly interested in high homology hits and the increased speed of Blat might be of value. Do you have any experience with Blat? If so how difficult do you think it would be to use it in an all vs all setup. Any comments would be great. I am a bit over my head at the moment. Thanks We have rocks 4.1 installed along with the biobrew roll. Currently we have about 14 cpus. Paul Mc Kenna Christopher Dwan wrote: > Sandy, > > First the nitty gritty: > -------------------------- > * I recommend using the binary downloads at ftp://ftp.ncbi.nih.gov/ > blast/. If computation is ever the limiting factor on your system then > switch over to custom binaries. > (snip)