[BiO BB] Primer BLAST
Bill_A_Nussbaumer at bd.com
Bill_A_Nussbaumer at bd.com
Thu Nov 21 14:08:39 EST 2002
Hi all,
I'm hoping a method exists that would allow me to align PCR primers against
the human genome to determine all products that could be amplified if my
sample contained extracted human DNA. Theoretically I could BLAST each
primer with stringent parameters and record the coordinates on the genome
looking for matches with a Kb or two of one another. To do that manually
would be quite tedious, especially when you consider that I'm only really
interested in matches at the 3' end.
Anyone know of a way to do this?
- Bill
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