[BiO BB] Primer BLAST

[Bill_A_Nussbaumer at bd.com]

Hi all,

I'm hoping a method exists that would allow me to align PCR primers against
the human genome to determine all products that could be amplified if my
sample contained extracted human DNA.  Theoretically I could BLAST each
primer with stringent parameters and record the coordinates on the genome
looking for matches with a Kb or two of one another.  To do that manually
would be quite tedious, especially when you consider that I'm only really
interested in matches at the 3' end.

Anyone know of a way to do this?

- Bill