[BiO BB] Primer BLAST
idoerg at burnham.org
Thu Nov 21 14:26:40 EST 2002
Have you considered downloading and installing BLAST locally, and then
writing a script to run all your primers and record their scores?
Bill_A_Nussbaumer at bd.com wrote:
> Hi all,
> I'm hoping a method exists that would allow me to align PCR primers against
> the human genome to determine all products that could be amplified if my
> sample contained extracted human DNA. Theoretically I could BLAST each
> primer with stringent parameters and record the coordinates on the genome
> looking for matches with a Kb or two of one another. To do that manually
> would be quite tedious, especially when you consider that I'm only really
> interested in matches at the 3' end.
> Anyone know of a way to do this?
> - Bill
> BiO_Bulletin_Board maillist - BiO_Bulletin_Board at bioinformatics.org
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