Hi to all, I am sending this e-mail so that other people besides me get the necessary insights into building a biocluster. I have divided my questions in multiple posts so that any input for specific point can be address in replying to the appropriate post. I am hoping to get some info that could than be use to create a web site that could help people build their own biocluster. Our present goal is to build a reasonably fast, easily expendable and affordable local BLAST server. We need it to be accessible both via a web page and a command-line interface (for batch jobs). We want it to get the daily updates from GenBank as well as use user-created databases. We might also extend its use to RNA folding and AI-based gene identification. It is also to be the central core of some research projects, including the development of administrative tools for cluster management and sequence analysis algorithms. More to come... Sylvain