Hi All, I solved the error, by specifying the matrix path in "BLASTMAT" environment varibale. but I want to specify the path in my ".ncbirc" file, my .ncbirc file content in my root directory is #cat .ncbirc [NCBI] Data="/usr/local/sge/db/data/" but still blastall is not taking the matrix file from that location, any suggestion?? help?? -bala- --- Bala <egrpbala@yahoo.com> wrote: > Hi All, > I can able to run blastall when the BLOSUM62 > file is in the local directory and I am getting the > results, if I try to run from some other location > and giving the path to the BLOSUM62 file it is > giving > the following error! > > Command: > ------- > #blastall -p blastp -d /usr/local/db/pataa -i > /usr/local/sge/QUERY -M /usr/local/db/BLOSUM62 > > Error: > ----- > [blastall] ERROR: gi|532319|pir|TVFV2E|TVFV2E: > BlastKarlinBlkGappedCalc: BLOSUM80 is a supported > matrix > [blastall] ERROR: gi|532319|pir|TVFV2E|TVFV2E: > BlastKarlinBlkGappedCalc: BLOSUM62 is a supported > matrix > [blastall] ERROR: gi|532319|pir|TVFV2E|TVFV2E: > BlastKarlinBlkGappedCalc: BLOSUM50 is a supported > matrix > [blastall] ERROR: gi|532319|pir|TVFV2E|TVFV2E: > BlastKarlinBlkGappedCalc: BLOSUM45 is a supported > matrix > [blastall] ERROR: gi|532319|pir|TVFV2E|TVFV2E: > BlastKarlinBlkGappedCalc: PAM250 is a supported > matrix > [blastall] ERROR: gi|532319|pir|TVFV2E|TVFV2E: > BlastKarlinBlkGappedCalc: BLOSUM62_20 is a supported > matrix[blastall] ERROR: gi|532319|pir|TVFV2E|TVFV2E: > BlastKarlinBlkGappedCalc: BLOSUM90 is a supported > matrix > [blastall] ERROR: gi|532319|pir|TVFV2E|TVFV2E: > BlastKarlinBlkGappedCalc: PAM30 is a supported > matrix > [blastall] ERROR: gi|532319|pir|TVFV2E|TVFV2E: > BlastKarlinBlkGappedCalc: PAM70 is a supported > matrix > --------------- > > In that error it is searching BLOSUM62 also, help > me solve this error. > > Thanks, > Bala. > > > __________________________________________________ > Do you Yahoo!? > Faith Hill - Exclusive Performances, Videos & More > http://faith.yahoo.com > _______________________________________________ > Bioclusters maillist - > Bioclusters@bioinformatics.org > https://bioinformatics.org/mailman/listinfo/bioclusters __________________________________________________ Do you Yahoo!? Faith Hill - Exclusive Performances, Videos & More http://faith.yahoo.com