Hi Joe, Thanks for the information, I found out the solution for that command line parameter problem, the command I am trying to run is #blastall -p blastp -d /usr/local/sge/db/pataa -i /usr/local/sge/QUERY -M /usr/local/sge/db/BLOSUM62 the solotion is, instead of giving the matrix path in the command line, set the enviroinment variable "BLASTMAT". I didn't find .ncbirc file in my stand allown blast download from NCBI site, can you give me some info regrading .ncbirc file content and where to put(location) of the file. Thanks, Bala. --- Joe Landman <landman at scalableinformatics.com> wrote: > Hi Bala: > > Look on http://www.ncbi.nlm.nih.gov for the BLAST > (standalone) bit. > > It looks to me like you may have some command line > parameter problems, > or problems with you .ncbirc file. The data.tar.Z > file on the ftp site > contains the scoring matrices, and the tarball > contains the code. > > What are you running, including the complete > command line? > > Joe > > On Wed, 2002-10-09 at 22:05, Bala wrote: > > Hi All, > > I am new to this list, > > I wondering is it source available > > for blastall executable from NCBI site. > > I am getting some error with the one > > of the parameter(Matrix value) to > > blastall. > > > > If that default file BLOSUM62 is > > available locally, then it is working, > > if I am running from some other location > > and specifing that file location, then it > > is giving error. > > > > [blastall] ERROR: gi|532319|pir|TVFV2E|TVFV2E: > > BlastKarlinBlkGappedCalc: test/BLOSUM62 is not a > > supported matrix > > > > any suggestion! help! > > > > -bala- > > > > __________________________________________________ > > Do you Yahoo!? > > Faith Hill - Exclusive Performances, Videos & More > > http://faith.yahoo.com > > _______________________________________________ > > Biodevelopers mailing list > > Biodevelopers at bioinformatics.org > > > https://bioinformatics.org/mailman/listinfo/biodevelopers > -- > Joseph Landman, Ph.D > email: landman at scalableinformatics.com > web : http://scalableinformatics.com > phone: +1 734 612 4615 > > _______________________________________________ > Biodevelopers mailing list > Biodevelopers at bioinformatics.org > https://bioinformatics.org/mailman/listinfo/biodevelopers __________________________________________________ Do you Yahoo!? Faith Hill - Exclusive Performances, Videos & More http://faith.yahoo.com