Hello, I am analyzing 2D gels and I obtain a lot of spots representing proteins to identify (with only 2 criteria : pI and Mw). For that purpose, I use TagIdent, a tool from Expasy (see http://www.expasy.org/tools/tagident.html). The thing is that I really have _a lot_ of spot to identify (at least 100). For the moment, I send queries to TagIdent during a half day for one gel. I was wondering if there is a tool to perform these queries in batch (I give a file with spotID, pI, Mw and the result is a list of possible proteins). I thought I can write it by myself and/or modify the TagIdent Perl script (http://www.expasy.org/cgi-bin/tagident0.pl). But I don't know if TagIdent is an open source / free software. And, if it is, I cannot obtain the source code. Did someone already have such problem or know the solution? I look at Google and BioPerl but I wasn't able to find anything. Thanks in advance, Jean-Etienne