-> Does anyone know of a tool to calculate the binding affinity between -> between a short mRNA probe and a gene? I am familiar with mfold and the -> vienna rna package, which perform similar calculations (correct me if I am -> wrong here), but they calculate energies for self-binding. Google for 'melting temperature' or 'annealing' instead. The first hit is this: http://www.retrogen.com/index/melting_template.htm cheers, --titus