Hi Ethan, We at the Genome Sciences Centre have developed a software tool called DiscoverySpace which can help you assign sub cellular localization to your Refseq gene list. Although we don't have the ability, as yet, to map genes directly to protein localization, our application does allow you to assign the list to both PSORT and PAGOSUB, both of which were mentioned earlier by Jenn Grady. In the case of PSORT we store the pre-computed results for all of the Refseq proteins, which makes the assignments very quick. Our application provides a user interface to over 10 of the more frequently used biological databases in the public domain which we have integrated into one large 'warehouse'. Some of the databases we support are GO, SwissProt, Refseq, Ensembl and MGC. More information about DiscoverySpace including primers, tutorials and downloads can be found at: http://www.bcgsc.ca/bioinfo/software/discoveryspace/ or feel free to contact us at discoveryspace-support at bcgsc.ca <mailto:discoveryspace-support at bcgsc.ca> Best Regards Richard Varhol Assistant Bioinformatics Coordinator Genome Sciences Centre Vancouver Canada -------------- next part -------------- An HTML attachment was scrubbed... URL: http://bioinformatics.org/pipermail/biodevelopers/attachments/20051130/82336b44/attachment.html