-----BEGIN PGP SIGNED MESSAGE----- Hash: SHA1 hua pan wrote: | I have a question about pathdb. Shall we download all the database file | from NCBI Blast website? It's huge and we also need to update often. You could do BLAST against any secuence database you want, not only NCBI NR database. The BLAST has a program called formatdb to convert any fasta file in a BLAST formated database. So you don't need to donwload any database from NCBI. Anyway, you could donload a small database, like e.coli or arabidopsis (they are way much smaller than nr database). - -- Best regards, //=\ Sebastian Bassi - Diplomado en Ciencia y Tecnologia, UNQ //=\ \=// IT Manager Advanta Seeds - Balcarce Research Center - \=// //=\ Pro secretario ASALUP - www.asalup.org - PGP key available //=\ \=// E-mail: sbassi@genesdigitales.com - ICQ UIN: 3356556 - \=// ~ http://Bioinformatica.info -----BEGIN PGP SIGNATURE----- Version: GnuPG v1.0.6 (MingW32) Comment: Using GnuPG with Mozilla - http://enigmail.mozdev.org iEYEARECAAYFAj9YzeEACgkQ6lc7ixf6gKo9ngCgzUsM0xE9mVfI+WQF41AEn3QS UboAoND9yXCmpYlgHdY6J2PnyqytA71c =IZEZ -----END PGP SIGNATURE-----