Hi Kevin, I started working on a PHP interface a couple years ago. I set it up on the MeltSim website just now for anyone who'd like to use it: http://bioinformatics.org/meltsim/online/ Note that there may be some kinks in it. On the back-end it's running version 1.99.0 (C/Linux), which is older and imperfect. Also, I'm not sure if the web server will handle very large jobs from MeltSim: there's no scheduler in place for it, and MeltSim can be very compute intensive. Try to keep the sequences under 10 kbp, especially if running a positional map. Please report any problems that you or anyone else finds, and I'll add this interface to the source code in SVN, just as soon as it seems ready: http://bioinformatics.org/websvn/listing.php?repname=meltsim&path=%2Ftrunk%2F&rev=0&sc=0 Cheers, Jeff Kevin Hollevoet wrote: > hello, > > i'm kevin hollevoet, thesisstudent at the university of Ghent (belgium) > at the medical research building. we are planning to use meltsim to > preform some large scale simulations to test the sensitivity of HRM > towards single CpG's methylation differences in NB cell lines. > > but the problems we are facing are the same like Marko Pavlekovic wrote > to you some time ago (for the record: i'm using window XP to run MELTSIM) > > so i tried, just like marko, to change all '.' into ','. even in the > output files, when i only selected the meltcurse, the graph was not > what i would expect. other options they gave also errors for the use of > E09 (meaning 10^9) in the output file. and that fault (i dont even know > what the fault is) is obviously not that easy to change in an output file. > > so is there already some other version on the market or the web? perhaps > this is some european problem with the configuration of the windows XP > here? as there are a lot of articles citing your program, even recently, > it should work somehow perfectly well, no? > > greetings > > kevin -- J.W. Bizzaro Bioinformatics Organization, Inc. (Bioinformatics.Org) E-mail: jeff at bioinformatics.org Phone: +1 508 890 8600 --