[meltsim] problem running MELSTIM

Marx, Kenneth Kenneth_Marx at uml.edu
Tue Aug 21 10:52:09 EDT 2007

Hi Kevin,

I don't know how you plan to do the study, but MELTSIM is not
parameterized for melting methylated bases. This is something that Prof.
Richard Blake, who developed and parameterized MELTSIM did not do. I
asked him years ago about this issue of melting methylated bases.

Good luck, Prof. Ken Marx

-----Original Message-----
From: meltsim-bounces+kenneth_marx=uml.edu at bioinformatics.org
[mailto:meltsim-bounces+kenneth_marx=uml.edu at bioinformatics.org] On
Behalf Of Kevin Hollevoet
Sent: Wednesday, August 15, 2007 12:10 PM
To: meltsim at bioinformatics.org
Subject: [meltsim] problem running MELSTIM


i'm kevin hollevoet, thesisstudent at the university of Ghent  
(belgium) at the medical research building. we are planning to use  
meltsim to preform some large scale simulations to test the  
sensitivity of HRM towards single CpG's methylation differences in NB  
cell lines.

but the problems we are facing are the same like Marko Pavlekovic  
wrote to you some time ago (for the record: i'm using window XP to run  

so i tried, just like marko, to change all '.' into ','. even in the  
output files,  when i only selected the meltcurse, the graph was not  
what i would expect. other options they gave also errors for the use  
of E09 (meaning 10^9) in the output file. and that fault (i dont even  
know what the fault is) is obviously not that easy to change in an  
output file.

so is there already some other version on the market or the web?  
perhaps this is some european problem with the configuration of the  
windows XP here? as there are a lot of articles citing your program,  
even recently, it should work somehow perfectly well, no?


meltsim maillist  -  meltsim at bioinformatics.org

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