Hi Kevin, I don't know how you plan to do the study, but MELTSIM is not parameterized for melting methylated bases. This is something that Prof. Richard Blake, who developed and parameterized MELTSIM did not do. I asked him years ago about this issue of melting methylated bases. Good luck, Prof. Ken Marx -----Original Message----- From: meltsim-bounces+kenneth_marx=uml.edu at bioinformatics.org [mailto:meltsim-bounces+kenneth_marx=uml.edu at bioinformatics.org] On Behalf Of Kevin Hollevoet Sent: Wednesday, August 15, 2007 12:10 PM To: meltsim at bioinformatics.org Subject: [meltsim] problem running MELSTIM hello, i'm kevin hollevoet, thesisstudent at the university of Ghent (belgium) at the medical research building. we are planning to use meltsim to preform some large scale simulations to test the sensitivity of HRM towards single CpG's methylation differences in NB cell lines. but the problems we are facing are the same like Marko Pavlekovic wrote to you some time ago (for the record: i'm using window XP to run MELTSIM) so i tried, just like marko, to change all '.' into ','. even in the output files, when i only selected the meltcurse, the graph was not what i would expect. other options they gave also errors for the use of E09 (meaning 10^9) in the output file. and that fault (i dont even know what the fault is) is obviously not that easy to change in an output file. so is there already some other version on the market or the web? perhaps this is some european problem with the configuration of the windows XP here? as there are a lot of articles citing your program, even recently, it should work somehow perfectly well, no? greetings kevin _______________________________________________ meltsim maillist - meltsim at bioinformatics.org https://bioinformatics.org/mailman/listinfo/meltsim