Hi Jeff, With the difference plot i mean that the differences in melting curve shape, who can be further analyzed by subtracting the curves from a reference curve. This helps cluster samples automatically into groups that have similar melting curves, so something else then the derivate profile, pos map and pos profile. and i think i made a fault before by presumig i could do it with the log data from meltsim because that are the derivate profile data. i'll send you a file about the HRM tool (light-cycler)we use in the lab, that way i can make myself totally clear. so i wondered if it is possible to simulate these difference plots with some software... kind regards, kevin Citeren "J.W. Bizzaro" <jeff at bioinformatics.org>: > Hi Kevin, > > Are you asking about a plot of the difference between two melting > curves (derivative profile, positional map, or positional profile?) or > something else? > > Jeff > > Kevin Hollevoet wrote: >> hello, >> >> i was wondering i you have within melstim any simulation option >> that can generate the difference plots? i think it is possible if i >> do it with the log data from different data in excel or some other >> program, but that is obviously quite some work. >> so simulation of some difference plot to show the possible >> clustering would be very attractive to us. because we are trying to >> show that HRM analysis of heterogene methylated NB cellines is not >> possible. in other words that the clustering would be >> insignificant to give some idea about the methylation status. >> >> could you give me a hint here? >> >> thank you, >> >> kevin > > -- > J.W. Bizzaro > Bioinformatics Organization, Inc. (Bioinformatics.Org) > E-mail: jeff at bioinformatics.org > Phone: +1 508 890 8600 > -- -------------- next part -------------- A non-text attachment was scrubbed... Name: LightCycler_HRM.doc Type: application/msword Size: 226816 bytes Desc: not available Url : http://bioinformatics.org/pipermail/meltsim/attachments/20070822/4c5210c6/LightCycler_HRM-0001.doc