Hi Kevin, Are you asking about a plot of the difference between two melting curves (derivative profile, positional map, or positional profile?) or something else? Jeff Kevin Hollevoet wrote: > hello, > > i was wondering i you have within melstim any simulation option that can > generate the difference plots? i think it is possible if i do it with > the log data from different data in excel or some other program, but > that is obviously quite some work. > so simulation of some difference plot to show the possible clustering > would be very attractive to us. because we are trying to show that HRM > analysis of heterogene methylated NB cellines is not possible. in other > words that the clustering would be insignificant to give some idea about > the methylation status. > > could you give me a hint here? > > thank you, > > kevin -- J.W. Bizzaro Bioinformatics Organization, Inc. (Bioinformatics.Org) E-mail: jeff at bioinformatics.org Phone: +1 508 890 8600 --