[MeltSim] calculating melting curve of mixture of 2 different DNAmolecules

[Marx, Kenneth]

Hi Petar,

MELTSIM Is not set up I believe to calculate two different molecules simultaneously. But why not just calculate each of them separately under identical condition? That is functionally equivalent to doing a mixture of the two. Whether you'll see much difference between the two with 1 nucleotide change remains to be seen.

Good luck   -Ken Marx

-----Original Message-----
From: meltsim-bounces at bioinformatics.org on behalf of Petar Ozretic
Sent: Thu 8/20/2009 5:48 AM
To: meltsim at bioinformatics.org
Subject: [MeltSim] calculating melting curve of mixture of 2 different DNAmolecules
 
I have one question!
Can you use MeltSim to calculate melting curve of mixture of 2  
different DNA molecules e.g. when you have a heterozygous gene and two  
alleles that differ in 1 nucleotide?

_______________________________________________
MeltSim maillist  -  MeltSim at bioinformatics.org
http://www.bioinformatics.org/mailman/listinfo/meltsim

-------------- next part --------------
An HTML attachment was scrubbed...
URL: <http://www.bioinformatics.org/pipermail/meltsim/attachments/20090820/fc65be99/attachment.html>