[MeltSim] calculating melting curve of mixture of 2 different DNAmolecules

[richard blake]

On Aug 20, 2009, at 8:50 AM, Marx, Kenneth wrote:

>
> To answer the last question you posed---yes.
>
> -Ken
>
> -----Original Message-----
> From: meltsim-bounces at bioinformatics.org on behalf of Petar Ozretic
> Sent: Thu 8/20/2009 8:36 AM
> To: meltsim at bioinformatics.org
> Subject: Re: [MeltSim] calculating melting curve of mixture of 2  
> different DNAmolecules
>
> Well yes, that would be logical approach but as in the high  
> resolution melting
> analysis of one gene, you should be able do distinct 3 different
> melt.curves (two for two types of homozygous alleles and one for
> heterozygous allele that is, by the way, in a test tube present as a
> mixture of 4 different kind of dsDNA molecules! So I'm wondering is a
> melting curve of mixture of 2 DNAs just a sum of
> 2 melting curves of each DNA alone?!
You are correct. Any melting curve is, of course, mostly just the sum  
of its parts. Melting curves of bacterial or viral DNAs are curves of  
collections of gene sequences. There is a small difference, however,  
due to end effects. (RDB)
>
>> Hi Petar,
>>
>> MELTSIM Is not set up I believe to calculate two different molecules
>> simultaneously. But why not just calculate each of them separately
>> under identical condition? That is functionally equivalent to doing
>> a mixture of the two. Whether you'll see much difference between the
>> two with 1 nucleotide change remains to be seen.
>>
>> Good luck   -Ken Marx
>
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