[MeltSim] calculating melting curve of mixture of 2 different DNAmolecules
Kenneth_Marx at uml.edu
Thu Aug 20 08:50:11 EDT 2009
To answer the last question you posed---yes.
From: meltsim-bounces at bioinformatics.org on behalf of Petar Ozretic
Sent: Thu 8/20/2009 8:36 AM
To: meltsim at bioinformatics.org
Subject: Re: [MeltSim] calculating melting curve of mixture of 2 different DNAmolecules
Well yes, that would be logical approach but as in the high resolution melting
analysis of one gene, you should be able do distinct 3 different
melt.curves (two for two types of homozygous alleles and one for
heterozygous allele that is, by the way, in a test tube present as a
mixture of 4 different kind of dsDNA molecules! So I'm wondering is a
melting curve of mixture of 2 DNAs just a sum of
2 melting curves of each DNA alone?!
> Hi Petar,
> MELTSIM Is not set up I believe to calculate two different molecules
> simultaneously. But why not just calculate each of them separately
> under identical condition? That is functionally equivalent to doing
> a mixture of the two. Whether you'll see much difference between the
> two with 1 nucleotide change remains to be seen.
> Good luck -Ken Marx
MeltSim maillist - MeltSim at bioinformatics.org
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