Hi Manisha, I am not sure what your problem is exactly, but computing pairwise global alignments of distantly related proteins makes little sense. In that case I would rather - for each pair - add a number (5-10) closer homologues for each protein (50-70% homology) and compute a multiple alignment for that set. Here T-Coffee would be my program of choice (it computes multiple alignment scores). On the other hand, if all of your 400 sequences are close homologues, I would use MUSCLE to align all 400 in one go. I would then use protdist from phylip to compute phylogenetic distances between all pairs if that is of use for your purpose. Good luck, Karsten. On Monday 10 January 2005 20:47, Goel, Manisha wrote: > Hi All, > > I have a database of about 400 squences, which I want to compare > All-vs-All to get the global alignment score of each protein sequence > pair. > I tried using bl2seq but that gives me local alignments. > What other program would suit this function best ? > I would probably like to have the flexibility of being able to use > various matrices. > > Thanks a lot, > -Manisha