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February 26, 2019, at 01:42 PM by 132.170.195.160 -
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Batches of whole proteomes (.FAA), lists of metabolic pathways, or lists of metabolic dependencies (aka: "seeds") and "non-seeds".

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Batches of whole proteomes (.faa), lists of metabolic pathways, or lists of metabolic dependencies (aka: "seeds") and "non-seeds".

February 26, 2019, at 01:42 PM by 132.170.195.160 -
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Distance matrices and pairwise lists of distances between each of the supplied proteomes/metabolomes in the batch. Competition and complementarity matrices are generated for seed/non-seed analyses.

to:

All output files will propagate in the ./outfiles/ subdirectory.

For the pairwise_prot_blast and pairwise_paths modules, output includes distance matrices and pairwise lists of distances between each of the supplied proteomes/metabolomes in the batch. Competition and complementarity matrices are generated for seed/non-seed analyses.

February 26, 2019, at 01:40 PM by 132.170.195.160 -
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Run the analyses using python2 <module_name>.py

to:

Run the analyses using

 > python2 <module_name>.py

February 26, 2019, at 01:39 PM by 132.170.195.160 -
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Proteomes can be downloaded using NCBI's WGS browser or can supplied by the user.

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Proteomes can be downloaded using NCBI's WGS Browser or can supplied by the user.

February 26, 2019, at 01:37 PM by 132.170.195.160 -
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For each module

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No installation is required to use the PhyloMetaProt modules.

Unzip the package into a desirable directory.

Place the files intended to be analyzed in the module's ./infiles/ subdirectory.

Using a command shell, navigate to the module's directory.

Run the analyses using python2 <module_name>.py

February 26, 2019, at 01:32 PM by 132.170.195.160 -
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ABOUT

PhyloMetaProt is written for Python 2.7 and uses a command line interface.

Parallel processing is supported for BLAST-based operations and is highly recommended.

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PhyloMetaProt is written for Python 2.7 and uses a command line interface.

Parallel processing is supported for BLAST-based operations and is highly recommended.

PhyloMetaProt requires the installation of the Python modules: numpy, pandas, and biopython. We recommend using pip2 to obtain packages. Users should check to ensure the packages are installed for Python 2.7, rather than Python 3.X

to:

PhyloMetaProt requires the installation of the Python modules: numpy, pandas, and biopython. We recommend using pip2 to obtain packages. Users should check to ensure the packages are installed for Python 2.7, rather than Python 3.X.

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Proteomes can be downloaded using NCBI's WGS browser or can supplied by the user.

The pairwise_paths module was intended for use with PathwayTools, but should also function with KEGG. Any exported list of metabolic pathways should work for input!

The pairwise_seeds module requires that SBML formatted reaction networks be pre-processed by NetSeed.

to:

Proteomes can be downloaded using NCBI's WGS browser or can supplied by the user.

The pairwise_paths module was intended for use with PathwayTools, but should also function with KEGG. Any exported list of metabolic pathways should work for input!

The pairwise_seeds module requires that SBML formatted reaction networks be pre-processed by NetSeed.

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USAGE

For each module

February 26, 2019, at 01:10 PM by 132.170.195.160 -
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PhyloMetaProt is written for Python 2.7 and uses a command line interface.

Parallel processing is supported for BLAST-based operations and is highly recommended.

to:
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PhyloMetaProt requires the installation of the Python modules: numpy, pandas, and biopython. We recommend using pip to obtain packages.

to:

PhyloMetaProt is written for Python 2.7 and uses a command line interface.

Parallel processing is supported for BLAST-based operations and is highly recommended.

PhyloMetaProt requires the installation of the Python modules: numpy, pandas, and biopython. We recommend using pip2 to obtain packages. Users should check to ensure the packages are installed for Python 2.7, rather than Python 3.X

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February 26, 2019, at 11:56 AM by 132.170.195.160 -
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The pairwise_seed module requires that SBML formatted reaction networks be pre-processed by NetSeed.

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The pairwise_seeds module requires that SBML formatted reaction networks be pre-processed by NetSeed.

February 26, 2019, at 11:56 AM by 132.170.195.160 -
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MODULES

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MODULES

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REQUIREMENTS

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REQUIREMENTS

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INPUT

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INPUT

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OUTPUT

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OUTPUT

February 25, 2019, at 10:06 PM by 99.117.188.235 -
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Proteomes can be downloaded using NCBI's WGS browser, or can supplied by the user.

to:

Proteomes can be downloaded using NCBI's WGS browser or can supplied by the user.

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For clustering and visualization of phylograms, we recommed the use of MEGA from NCBI.

to:

For clustering and visualization of phylograms we recommend the use of MEGA from NCBI.

February 25, 2019, at 10:02 PM by 99.117.188.235 -
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PhyloMetaProt is written for Python 2.7 and uses a command line interface.

Parallel processing is supported for BLAST-based operations and is highly recommended.

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UNDER THE HOOD

PhyloMetaProt is written for Python 2.7 and uses a command line interface.

Parallel processing is supported for BLAST-based operations and is highly recommended.

to:
February 25, 2019, at 09:59 PM by 99.117.188.235 -
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This is a default page.if ! auth edit

Loginifend

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MODULES

pairwise_prot_blast - Comprehensive BLASTp distance analysis between whole proteomes

pairwise_paths - Distance analysis from metabolic pathways

pairwise_seeds - Competition and complementarity of metabolic dependencies (seeds)

UNDER THE HOOD

PhyloMetaProt is written for Python 2.7 and uses a command line interface.

Parallel processing is supported for BLAST-based operations and is highly recommended.

REQUIREMENTS

PhyloMetaProt requires the installation of the Python modules: numpy, pandas, and biopython. We recommend using pip to obtain packages.

The pairwise_prot_blast module also requires installation of NCBI's BLAST executables.

INPUT

Batches of whole proteomes (.FAA), lists of metabolic pathways, or lists of metabolic dependencies (aka: "seeds") and "non-seeds".

Proteomes can be downloaded using NCBI's WGS browser, or can supplied by the user.

The pairwise_paths module was intended for use with PathwayTools, but should also function with KEGG. Any exported list of metabolic pathways should work for input!

The pairwise_seed module requires that SBML formatted reaction networks be pre-processed by NetSeed.

Example input data is included for all three modules.

OUTPUT

Distance matrices and pairwise lists of distances between each of the supplied proteomes/metabolomes in the batch. Competition and complementarity matrices are generated for seed/non-seed analyses.

For clustering and visualization of phylograms, we recommed the use of MEGA from NCBI.