[BiO BB] Assemble forward/reverse sequence trace data
Diego Martinez
sariego9 at yahoo.com
Fri Jul 22 08:19:50 EDT 2005
Hello Martin,
The classic trace aligner is the Phred-Phrap-Consed
package from Phil Green up at UW. it claims it
now works on Windows, however, that may mean you have
to compile it yourself. I have performed this successfully
on Sun, SGI and Linux, but never on Windows.
check it out at:
http://www.phrap.org/phredphrapconsed.html
Diego
--- Corné HW Klaassen <c.klaassen at cwz.nl> wrote:
> Hi Martin,
>
> Thanks for this quick reaction, but I'm more looking for a software
> package that lets me align the forward and reverse traces to produce and
> export the consensus sequence. I can easily do this with the sequence
> (text) itself using ClustalX but I would prefer to do this with the
> sequence traces so that I have a visual check of the bases that have
> been called.
>
> Corné
>
> Martin Luetzelberger wrote:
>
> > FinchTV might be what you want.
> > http://www.geospiza.com/finchtv/features/index.html
> >
> > At 09:45 AM 7/22/2005, you wrote:
> >
> >> Hi All,
> >>
> >> Can anybody recommend a free software tool (windows compatible) that
> >> allows the user to combine forward and reverse sequence trace data
> >> into a final assembly?
> >>
> >> Thanks,
> >>
> >> Corné
> >>
> >> -----------------------------------------------------------------------------------------
> >>
> >> dr. Corné H.W. Klaassen, Molecular Biologist
> >> Canisius Wilhelmina Hospital, Molecular Biology Unit, C60-C70
> >> Weg door Jonkerbos 100, 6532 SZ Nijmegen, The Netherlands.
> >>
> >> tel: #31-24-3658670 (office)
> >> tel: #31-24-3658677 (direct)
> >> fax: #31-24-3658671
> >> E-Mail: c.klaassen at cwz.nl
> >>
> >> _______________________________________________
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> >
> >
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