I am still trying to figure out the numerology behind mpiblast. I haven't applied the patches to mpiformatdb yet. 16 nodes dual cpu 2.4G XEON 1 node dual cpu 2.4G XEON That makes 34 cpus total. So is the N the number of cpu's ( minus 1 or 2) in the mpiformatdb command or the number of dual nodes? Jeremy seemed to do well with the number of cpus, but I'm not getting results that way. On the other hand, using 30 as N for mpiformatdb (31 sections of nr) and mpirun -np 16 mpiblast -p blastp -d nr -a 2 -i in.file -o out.file gives me about 4-5 sec per query, using the -a 2 (use 2 cpus) flag. Also I am curious about Eric Engelhard's statement last year ( Mar01,2002) "but my work cluster (18 nodes 2GB RAM/node) cuts through >1500 queries/minute against nr." That's 40 msec per query. He didn't indicate running special hardware. If that is true how is that achieved? It's >100 times what people ( Jeremy, John) have been reporting recently. David -------- David Lapointe Information Services UMass Medical School Worcester MA 01655