Dear all, While trying to create a rpsblast searchable profile database, I am facing problems with Secondary preprocessing of the files with copymat. It is throwing the following error - [copymat] FATAL ERROR: Failed to allocate -1554705136 bytes How to solve this memory problem. I tried running this on a P4 with 512 MB RAM also. Thanks in advance. best Rajesh -- Ratna Rajesh Thangudu Research Scholar Laboratoire de Biochimie et Génétique Moléculaire Université de La Réunion 15 avenue René Cassin, BP 7151 97715 Saint Denis Messag Cedex 09 La Réunion, France Tel : +262 262 93 8641 Fax : +262 262 93 8237 Email : rajesh.thangudu at univ-reunion.fr ratnarajesh at yahoo.com