[Bio-Linux] NGS and GALAXY

[Andrei Zlobin]

Dear Experts,
I installed last version of Bio (on February) as a dual setup on a computer.
I need to work with data of RNA-Seq. Experiments were done on HiSeq2500 unit. I have all data, they are all right. I downloaded Bio-Linux fron DVD
disk,  installed on a computer, with 8G RAM, 4 core processor and 128 G disk space. I did update as recommended, all went fine.
 
1. I am using NGS programs, but problems start from very beginning, I can not  upload files, (they are big~3GB), when I press "execute" panel with
arrow blinks, (on right pane) but nothing is happening and it may continue for 48 h! If I use (usegalaxy.org with the same file all is all right.) I can
upload small files ~.5G but,
2. If file is small I can not run it because when I need to chose reference geneome, in option   "GENOME", it doesn't work-nothing is in the frame, (when I upload file option GENOME works fine). Also in some NGS programs option to choose file doesn't work. It doesn't see file at all.  
As far as I can see (I am a molecular biologist, not a IT tech) this is the problem of installation. I tried to read manuals, but it sad open file XYZ.loc (or ABC.ini)  ore something like this, which I can not fined at all, nothing like this.
Any suggestions?
Thanks
Andrew
 
 
 
Can you explain what's wrong?
 
 
-----------------------------
Andrew Zlobin, Ph.D
Cancer Center, Loyola University Medical Center
2160 S. First Ave, Bldg 112,
Rm 323,
Maywood, IL 60153
(708) 327-3122 
(708) 327-3342 fax
mailto:azlobin at lumc.edu 
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