PredictBias

identification of genomic and pathogenicity islands in prokaryotic genome

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A) Input parameters
Genome	1696.gbk	Threshold dinucleotide bias	2
Threshold codon bias	4	Threshold %GC bias	3
E-value (RPSBlast)	0.05	Genome (non-pathogenic)

B) Compare a potential GI or PAI in related non-pathogenic sp. (phylogenetic tree)
Potential GI or PAI start end
Select Organism

C) Potential GIs and PAIs in NC_008611 (download)

S.No

Start

End

Bias

Virulence

Insertion elements

Prediction

MUL_0022

MUL_0058

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
MUL_0022	2	13	-0.416175	serine/threonine phosphatase PstP
MUL_0023	1	14	-0.324086	hypothetical protein
MUL_0024	0	14	0.053209	hypothetical protein
MUL_0025	0	12	-0.767119	transposase for IS2606
MUL_0027	1	14	-0.147584	**glycosylase
MUL_0028	0	16	-0.870908	short-chain dehydrogenase
MUL_0029	2	13	-0.293605	hypothetical protein
MUL_0032	2	11	-0.660078	hypothetical protein
MUL_0033	2	12	-0.938948	osmoprotectant (glycine
MUL_0034	2	14	-0.459842	ABC transporter permease
MUL_0035	2	14	0.232183	DNA-binding protein
MUL_0036	3	14	1.078332	hypothetical protein
MUL_0037	2	13	1.760739	ammonium-transport integral membrane protein,
MUL_0038	1	13	2.029157	hypothetical protein
MUL_0039	1	13	2.384935	hypothetical protein
MUL_0040	2	14	2.078472	transcriptional regulatory protein (Whib-like),
MUL_0041	4	16	1.705989	transcriptional regulatory protein
MUL_0042	2	17	1.974343	secreted protein P60-related protein
MUL_0043	3	16	1.034365	hypothetical protein
MUL_0044	2	18	0.845713	hypothetical protein
MUL_0045	-1	14	1.315147	hypothetical protein
MUL_0046	1	14	1.296424	hypothetical protein
MUL_0047	1	14	1.279187	hypothetical protein
MUL_0048	1	13	0.665447	hypothetical protein
MUL_0049	2	13	1.119387	hypothetical protein
MUL_0050	1	13	2.520044	hypothetical protein
MUL_0051	-1	11	0.710198	integral membrane protein
MUL_0052	-2	10	0.405408	hypothetical protein
MUL_0053	-1	10	0.488451	hypothetical protein
MUL_0054	-1	9	0.362207	hypothetical protein
MUL_0055	-1	9	0.222957	secreted proline rich protein Mtc28
MUL_0056	-1	8	-0.728496	leucyl-tRNA synthetase
MUL_0057	2	13	-0.330399	short chain dehydrogenase
MUL_0058	2	13	0.261087	transcriptional regulatory protein

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_0022	PF03544	33	0.002	Gram-negative bacterial tonB protein
>PF03544#Gram-negative bacterial tonB protein Length = 243 Score = 33.0 bits (75), Expect = 0.002 Identities = 21/91 (23%), Positives = 27/91 (29%) Query: 419 PPCPAPRATSPPEPSAPSTASETPGQPSVTSSPASTTTPTPSASTTPTTTSGSTAATSTP 478 PP P PEP P P + ++P Sbjct: 69 PPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKPVKKVEQPKRDVKPVESRPASP 128 Query: 479 PAGTSPAAPTSPTPTSTTSSPNVTALPPPPP 509 T+PA PTS T T+ TS P + P Sbjct: 129 FENTAPARPTSSTATAATSKPVTSVASGPRA 159 Score = 30.7 bits (69), Expect = 0.011 Identities = 11/71 (15%), Positives = 26/71 (36%) Query: 420 PCPAPRATSPPEPSAPSTASETPGQPSVTSSPASTTTPTPSASTTPTTTSGSTAATSTPP 479 P P+ P+P + +P + + P+ T+ T T+ ++ ++ Sbjct: 95 EKPKPKPKPKPKPVKKVEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVA 154 Query: 480 AGTSPAAPTSP 490 +G + P Sbjct: 155 SGPRALSRNQP 165
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_0023	IGASERPTASE	30	0.005	IgA-specific serine endopeptidase (S6) signature.
>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature. Length = 1541 Score = 29.6 bits (66), Expect = 0.005 Identities = 15/55 (27%), Positives = 22/55 (40%) Query: 88 ADDSTLVLTDDYASTRHARLSQRGSEWYVEDLGSTNGTYLDRAKVTAAVRVPMGT 142 +D T +DY R + + S GTY D+ K A VR+ G+ Sbjct: 154 TEDQTQKRREDYYMPRLDKFVTEVAPIEASTASSDAGTYNDQNKYPAFVRLGSGS 208
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_0028	DHBDHDRGNASE	42	9e-07	2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase signature. Length = 261 Score = 41.6 bits (97), Expect = 9e-07 Identities = 29/131 (22%), Positives = 50/131 (38%) Query: 29 PADATNPVAAQSVVADVLERHGKIDVVLLNAGGAPALDLTRLDAADITSVMDANYDVAVN 88 PAD + A + A + G ID+++ AG + L + + N N Sbjct: 63 PADVRDSAAIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGVFN 122 Query: 89 YLVPVLRHMAERGTGVIAHTNSLARWYAVPLQGPYSAAKAALGVLFDAYRIEYAGSGVRF 148 V ++M +R +G I S Y+++KAA + +E A +R Sbjct: 123 ASRSVSKYMMDRRSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLELAEYNIRC 182 Query: 149 VSIYPGFVATE 159 + PG T+ Sbjct: 183 NIVSPGSTETD 193
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_0037	CHANLCOLICIN	32	0.005	Channel forming colicin signature.
>CHANLCOLICIN#Channel forming colicin signature. Length = 522 Score = 32.0 bits (72), Expect = 0.005 Identities = 12/37 (32%), Positives = 19/37 (51%) Query: 265 AASGVVAGLVAITPSCGTVNPLGAALIGLAAGIVCAF 301 AA V+ +VA+ S LG I + GI+C++ Sbjct: 471 AADAGVSYVVALLFSLLAGTTLGIWGIAIVTGILCSY 507
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_0039	TETREPRESSOR	28	0.045	Tetracycline repressor protein signature.
>TETREPRESSOR#Tetracycline repressor protein signature. Length = 218 Score = 28.0 bits (62), Expect = 0.045 Identities = 9/21 (42%), Positives = 10/21 (47%) Query: 73 HRDSAGVHARIRPGELNMMTA 93 +RD A VH RP E T Sbjct: 93 YRDGAKVHLGTRPDEKQYDTV 113
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_0050	PF04183	31	0.003	IucA / IucC family
>PF04183#IucA / IucC family Length = 580 Score = 31.4 bits (71), Expect = 0.003 Identities = 21/119 (17%), Positives = 30/119 (25%), Gaps = 7/119 (5%) Query: 126 MSAASMATARLMETWAHGLDVADALGVTRPATDRLRSIAHLGVRTRDYAYFVNNLAPPTE 185 + +A L W + DA V L A V YA E Sbjct: 287 IPGRYIAAGPLASRWLQQVFATDATLVQ-SGAVILGEPAAGYVSHEGYAALARAPYRYQE 345 Query: 186 QFLVELRGPGGDLWSWGPADAAQRVTGPAEDFCFLVTQRRPLSSLHLTATGEDARQWLQ 244 V R + +PL+ ++ +G DA WL Sbjct: 346 MLGVIWRE------NPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLDAETWLT 398
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_0057	DHBDHDRGNASE	89	1e-23	2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase signature. Length = 261 Score = 89.0 bits (220), Expect = 1e-23 Identities = 58/188 (30%), Positives = 85/188 (45%), Gaps = 13/188 (6%) Query: 4 ALITGASRGIGAAIAAAL--APTHTLLLAGRPSAQLDAV----ADQFGATTFPLDLTDDE 57 A ITGA++GIG A+A L H + P V A+ A FP D+ D Sbjct: 11 AFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHAEAFPADVRDSA 70 Query: 58 SIETSC----EIVDELDVLIHNAGLSIPGHFGDSHVDEWRATFNVNLFGAVALTLALLPA 113 +I+ + +D+L++ AG+ PG +EW ATF+VN G + ++ Sbjct: 71 AIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGVFNASRSVSKY 130 Query: 114 PRSAR-GQVVFINSGAGRNVSAGMASYSASKFALRAFADSLRTD--ESTLRVTTVYPGRT 170 R G +V + S MA+Y++SK A F L + E +R V PG T Sbjct: 131 MMDRRSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLELAEYNIRCNIVSPGST 190 Query: 171 DTDMQREL 178 +TDMQ L Sbjct: 191 ETDMQWSL 198

MUL_0070

MUL_0076

Genomic Island

LocusTag	DNBias	CDNBias	%GCBias	Product
MUL_0070	2	15	-0.566861	transcriptional regulatory protein
MUL_0071	4	17	0.087296	30S ribosomal protein S6
MUL_0072	2	14	0.621354	single-stranded DNA-binding protein
MUL_0073	4	13	0.328897	30S ribosomal protein S18
MUL_0074	4	12	0.608059	50S ribosomal protein L9
MUL_0075	4	14	0.282495	replicative DNA helicase DnaB
MUL_0076	2	16	0.477288	non-IS element not present in Mycobacterium

MUL_0383

MUL_0402

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
MUL_0383	3	10	1.586185	isopentenyl pyrophosphate isomerase
MUL_0384	4	14	2.572825	hypothetical protein
MUL_0385	2	12	2.395322	hypothetical protein
MUL_0387	3	12	2.261697	L-asparagine permease AnsP1
MUL_0389	0	15	2.572565	hypothetical protein
MUL_0391	0	15	2.032393	hypothetical protein
MUL_0392	2	15	-0.066403	transposase for IS2404
MUL_0393	0	13	-0.391588	hypothetical protein
MUL_0394	0	14	-0.520229	PE-PGRS family protein
MUL_0395	0	13	-0.514523	cold shock protein a, CspA
MUL_0396	-1	18	-1.693284	ATP-dependent RNA helicase, RhlE1
MUL_0397	-1	15	-1.401956	hypothetical protein
MUL_0398	-1	15	-1.005477	B12-dependent methionine synthase
MUL_0399	1	13	1.007050	hypothetical protein
MUL_0400	2	11	0.881325	hypothetical protein
MUL_0401	3	13	0.505061	transposase for IS2404
MUL_0402	2	13	0.218963	PPE family protein

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_0391	cloacin	36	4e-04	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 35.8 bits (82), Expect = 4e-04 Identities = 37/114 (32%), Positives = 43/114 (37%), Gaps = 7/114 (6%) Query: 173 GDGGPGSPGAASFDPTVAGGAGGPGGDARGIGDGGRGGDGGPGATGAPGGRGSDGGPGGK 232 GDG + GA S + GG G G GG G + P G GS G Sbjct: 4 GDGRGHNTGAHSTSGNINGGPTGLGVG------GGASDGSGWSSENNPWGGGSGSGIH-W 56 Query: 233 GGNAGDYGTGGTGGTGGIGGAGGPGSPGGTPGAQGFRAGDAGNGGVGGIGGDGG 286 GG +G GG G +GG G GG S P A GF A G + G Sbjct: 57 GGGSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAG 110 Score = 34.7 bits (79), Expect = 9e-04 Identities = 30/106 (28%), Positives = 34/106 (32%), Gaps = 1/106 (0%) Query: 222 GRGSDGGPGGKGGNAGDYGTGGTGGTGGIGGAGGPGSPGGTPGAQGFRAGDAGNGGVGGI 281 GRG + G GN TG G G G+G G G G G G Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGN- 64 Query: 282 GGDGGHIKGHGGAGGIGGTGGAGVIGGDGVIGGDGQAGSAGEFPGD 327 GG G+ G G GG A V G + G G A Sbjct: 65 GGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAG 110 Score = 33.9 bits (77), Expect = 0.002 Identities = 23/68 (33%), Positives = 29/68 (42%) Query: 405 SGGQGGLNGDGATRAAHGVQGTMGDGGDGGDGGNGSTTSDQVDIDGGNGGYGGWGFNGGN 464 SGG G + GA + + G G GG +GS S + + GG G G G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 465 GGNGGDGG 472 GNGG G Sbjct: 62 HGNGGGNG 69 Score = 32.0 bits (72), Expect = 0.006 Identities = 35/108 (32%), Positives = 38/108 (35%), Gaps = 14/108 (12%) Query: 328 RGGSGGAGARGGN--GGDGGAGGAGGHALAEGFHDGAAGTGGMGGDGGNGADGGDGHSGD 385 RG + GA + GN GG G G GG + G G N GG SG Sbjct: 7 RGHNTGAHSTSGNINGGPTGLGVGGG------------ASDGSGWSSENNPWGGGSGSGI 54 Query: 386 PSWRSGGDGGNGGNGAYGGSGGQGGLNGDGATRAAHGVQGTMGDGGDG 433 G G GGNG GG G GG A A G G G Sbjct: 55 HWGGGSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG 102 Score = 30.8 bits (69), Expect = 0.015 Identities = 24/76 (31%), Positives = 30/76 (39%), Gaps = 1/76 (1%) Query: 369 GGDGGNGADGGDGHSGDPSWRSGGDGGNGGNGAYGGSGGQGGLNGDGATRAAH-GVQGTM 427 GGDG G SG+ + G G GG G + G G+ H G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 428 GDGGDGGDGGNGSTTS 443 G+GG G+ G GS T Sbjct: 63 GNGGGNGNSGGGSGTG 78 Score = 30.1 bits (67), Expect = 0.026 Identities = 27/91 (29%), Positives = 32/91 (35%), Gaps = 2/91 (2%) Query: 270 AGDAGNGGVGGIGGDGGHIKGHGGAGGIGGTGGAGV-IGGDGVIGGDGQAGSAGEFPGD- 327 +G G G G G+I G G+GG G + G G G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 328 RGGSGGAGARGGNGGDGGAGGAGGHALAEGF 358 G GG G GG G GG A +A GF Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAAPVAFGF 92 Score = 29.7 bits (66), Expect = 0.038 Identities = 22/72 (30%), Positives = 30/72 (41%), Gaps = 5/72 (6%) Query: 483 SGGDGGFGGWGGNGYGGVGGGNGGAGGTAYTS-----GGVYAGGPVQPGTEGTGPSGDHG 537 + G G G G+G G G + G+ ++S GG G G G G G +G Sbjct: 10 NTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNG 69 Query: 538 GFGGSGGIGGHG 549 GG G GG+ Sbjct: 70 NSGGGSGTGGNL 81
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_0397	FLGHOOKFLIK	30	0.006	Flagellar hook-length control protein signature.
>FLGHOOKFLIK#Flagellar hook-length control protein signature. Length = 375 Score = 29.8 bits (66), Expect = 0.006 Identities = 11/44 (25%), Positives = 15/44 (34%), Gaps = 3/44 (6%) Query: 123 PGVPTPPAITGM--SPQEVTHALFGFRRQLAAQLAIYAPDRVPG 164 PG P +T + F + + QL PD PG Sbjct: 139 PGFDNTPKVTDAPSTVLPTEKPTL-FTKLTSEQLTTAQPDDAPG 181
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_0398	IGASERPTASE	30	0.016	IgA-specific serine endopeptidase (S6) signature.
>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature. Length = 1541 Score = 30.0 bits (67), Expect = 0.016 Identities = 17/51 (33%), Positives = 20/51 (39%), Gaps = 3/51 (5%) Query: 283 VTFDEDRHRAHTGNGAQVLATLRNTAINLHRLNGADNIAEACRITALTANR 333 V E+ H TGN L N I+ LN ADN + LT N Sbjct: 846 VRLTENSHWHLTGNSDVHQLDLANGHIH---LNSADNSNNVTKYNTLTVNS 893

MUL_0456

MUL_0474

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
MUL_0456	0	11	3.314573	transposase for IS2404
MUL_0457	0	11	2.785195	hypothetical protein
MUL_0458	0	11	2.985022	arylsulfatase AtsD
MUL_0459	0	11	2.543810	putative OHCU decarboxylase
MUL_0460	-1	11	1.140463	hypothetical protein
MUL_0462	-1	12	0.929047	hypothetical protein
MUL_0463	-1	11	0.272545	carbon monoxide dehydrogenase
MUL_0464	1	13	0.382918	hypothetical protein
MUL_0465	2	16	-0.868919	*hypothetical protein
MUL_0467	2	15	-0.733897	two component system response phosphate regulon
MUL_0468	1	15	-2.229912	two component system response phosphate sensor
MUL_0469	1	13	-1.592732	hypothetical protein
MUL_0470	3	12	-2.072086	hypothetical protein
MUL_0471	3	11	-1.663546	hypothetical protein
MUL_0472	2	9	-1.029604	hypothetical protein
MUL_0473	2	9	-1.150161	zinc-containing alcohol dehydrogenase NAD-
MUL_0474	2	10	-0.475283	hypothetical protein

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_0463	HTHFIS	108	2e-29	FIS bacterial regulatory protein HTH signature.
>HTHFIS#FIS bacterial regulatory protein HTH signature. Length = 484 Score = 108 bits (271), Expect = 2e-29 Identities = 36/136 (26%), Positives = 63/136 (46%) Query: 14 ARILVVDDEDNIVELLSVSLKFQGFEVHTATNGAQALDRARETRPDAVILDVMMPGMDGF 73 A ILV DD+ I +L+ +L G++V +N A D V+ DV+MP + F Sbjct: 4 ATILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAF 63 Query: 74 GVLRRLQADGIDAPALFLTARDSLQDKIAGLTLGGDDYVTKPFSLEEVVARLRVILRRAG 133 +L R++ D P L ++A+++ I G DY+ KPF L E++ + L Sbjct: 64 DLLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAEPK 123 Query: 134 KGSAEPRNSRLTFADI 149 + ++ + + Sbjct: 124 RRPSKLEDDSQDGMPL 139
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_0464	PF06580	32	0.004	Sensor histidine kinase
>PF06580#Sensor histidine kinase Length = 349 Score = 32.1 bits (73), Expect = 0.004 Identities = 21/114 (18%), Positives = 36/114 (31%), Gaps = 28/114 (24%) Query: 361 PRLRQVLSNLVGNALQH----TPDSADVTVRVGTAGENAVLEVADKGPGMPAEDAARVFE 416 P + ++ LV N ++H P + ++ LEV + G Sbjct: 256 PPM--LVQTLVENGIKHGIAQLPQGGKILLKGTKDNGTVTLEVENTGSLALKNT------ 307 Query: 417 RFYRTDSSRARASGGTGLGLSIVHS-LVKAHGGD--VTLTTAPGEGCCFRVTLP 467 TG GL V L +G + + L+ G+ V +P Sbjct: 308 ------------KESTGTGLQNVRERLQMLYGTEAQIKLSEKQGKVNA-MVLIP 348
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_0474	DHBDHDRGNASE	111	2e-31	2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase signature. Length = 261 Score = 111 bits (278), Expect = 2e-31 Identities = 70/229 (30%), Positives = 112/229 (48%), Gaps = 4/229 (1%) Query: 13 AIVAGASPGIGAATAVDLAAHGFPDALGARRVQKCEKIVEKIRADGGDAVALALDVTDAD 72 A + GA+ GIG A A LA+ G A +K EK+V ++A+ A A DV D+ Sbjct: 11 AFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHAEAFPADVRDSA 70 Query: 73 SVKDFVHQATERLGDIEVLVAGAGDTYFGRLYEIDTETFESQVQIHLIGANRLATAVLPG 132 ++ + + +G I++LV AG G ++ + E +E+ ++ G + +V Sbjct: 71 AIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGVFNASRSVSKY 130 Query: 133 MLERQRGDLIFVGSDVALRQRPHMGAYGAAKAALVAMVTNLQMELEGTGLRASIVHPGRT 192 M++R+ G ++ VGS+ A R M AY ++KAA V L +EL +R +IV PG T Sbjct: 131 MMDRRSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLELAEYNIRCNIVSPGST 190 Query: 193 KTAMGWSLPVESIGPALEDWAKWGQARHDYFL----RASDIARAITFVA 237 +T M WSL + G + L + SDIA A+ F+ Sbjct: 191 ETDMQWSLWADENGAEQVIKGSLETFKTGIPLKKLAKPSDIADAVLFLV 239

MUL_0499

MUL_0524

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
MUL_0499	3	12	0.342782	transmembrane protein
MUL_0500	2	13	0.139966	phosphoribosylaminoimidazole-succinocarboxamide
MUL_0501	2	14	-0.273822	protease II (oligopeptidase B), PtrB
MUL_0502	1	15	-0.469980	hypothetical protein
MUL_0503	2	20	-1.408352	transcriptional regulator
MUL_0504	1	23	-2.848454	integral membrane drug efflux protein
MUL_0505	2	22	-2.970324	hypothetical protein
MUL_0506	3	23	-3.680882	hypothetical protein
MUL_0507	3	21	-1.255274	hypothetical protein
MUL_0508	5	22	-0.761982	hypothetical protein
MUL_0509	4	20	-1.551869	N-term transposase for IS2404
MUL_0510	4	21	-2.621249	C-term transposase for IS2404
MUL_0511	4	20	-2.633899	Prophage phiMU01
MUL_0512	4	21	-2.607931	non-IS element not present in Mycobacterium
MUL_0513	1	21	-4.211734	hypothetical protein
MUL_0515	0	20	-4.522667	excisionase
MUL_0516	1	19	-4.451710	hypothetical protein
MUL_0517	0	19	-3.814834	hypothetical protein
MUL_0518	0	19	-3.631214	hypothetical protein
MUL_0520	-1	19	-3.802592	hypothetical protein
MUL_0521	1	21	-3.294615	hypothetical protein
MUL_0523	3	23	-2.764807	hypothetical protein
MUL_0524	3	19	-2.335321	hypothetical protein

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_0500	HTHTETR	53	6e-11	TetR bacterial regulatory protein HTH signature.
>HTHTETR#TetR bacterial regulatory protein HTH signature. Length = 215 Score = 53.5 bits (128), Expect = 6e-11 Identities = 29/122 (23%), Positives = 48/122 (39%), Gaps = 7/122 (5%) Query: 12 RAVILDEAARLVAQRGADRVSMRELARDAGVSHAAPAHHFTDRRGLFTALATQGFQLLAA 71 R ILD A RL +Q+G S+ E+A+ AGV+ A HF D+ LF+ + + Sbjct: 13 RQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSEIWELSESNIGE 72 Query: 72 ALVAARGHFADAALAYVRFAIEH-------PGHYQVMFNRSLHDADDADLAAAQAAAAAE 124 + + F L+ +R + H +++ H + A A Sbjct: 73 LELEYQAKFPGDPLSVLREILIHVLESTVTEERRRLLMEIIFHKCEFVGEMAVVQQAQRN 132 Query: 125 LA 126 L Sbjct: 133 LC 134
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_0501	TCRTETB	128	3e-34	Tetracycline resistance protein TetB signature.
>TCRTETB#Tetracycline resistance protein TetB signature. Length = 458 Score = 128 bits (324), Expect = 3e-34 Identities = 84/411 (20%), Positives = 172/411 (41%), Gaps = 19/411 (4%) Query: 47 VCVLASLMSVLDATVVGIAQRTFIIEFDSTQAVVAWTMTGYTLALATVIPVAGWAADRFG 106 +C+L S SVL+ V+ ++ +F+ A W T + L + V G +D+ G Sbjct: 19 LCIL-SFFSVLNEMVLNVSLPDIANDFNKPPASTNWVNTAFMLTFSIGTAVYGKLSDQLG 77 Query: 107 TKRLWMGSVLAFALGSLLCALAPNILS-LIFFRVLQGVGGGMLMPLGFIILTRAAGPKRL 165 KRL + ++ GS++ + + S LI R +QG G L +++ R + Sbjct: 78 IKRLLLFGIIINCFGSVIGFVGHSFFSLLIMARFIQGAGAAAFPALVMVVVARYIPKENR 137 Query: 166 GRLMAAIGIPMLLGPIGGPILGGWLISSFGWHWIFLVNLPIGLAAFALAAITFPADRPVP 225 G+ IG + +G GP +GG + HW +L+ +P+ + Sbjct: 138 GKAFGLIGSIVAMGEGVGPAIGGMIAHYI--HWSYLLLIPMITIITVPFLMKLLKKEVRI 195 Query: 226 SESFDVIGVLLLSPGLAAFLLALSLIPDRGTVADRYVLIPVIAGLSLIGGFAWHAWHRAD 285 FD+ G++L+S G+ F+L + + + ++++ V++ F H + Sbjct: 196 KGHFDIKGIILMSVGIVFFMLFTT------SYSISFLIVSVLS----FLIFVKHI-RKVT 244 Query: 286 HPLIDLHLFNNSVVTQANLTLLAFAAAYFGSSLLIPTYLQQVLHQTPMQSG-LYLIPQGL 344 P +D L N L G ++P ++ V + + G + + P + Sbjct: 245 DPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVSMVPYMMKDVHQLSTAEIGSVIIFPGTM 304 Query: 345 GAMLTMPIASAFMDRRGPGKSVLLGIVLIAVGLAMFTFGVATRAQYLPTLLVGLAIMGMG 404 ++ I +DRRGP + +G+ ++V +F + T + + + + + + G Sbjct: 305 SVIIFGYIGGILVDRRGPLYVLNIGVTFLSVSFLTASFLLETTSWF---MTIIIVFVLGG 361 Query: 405 TGCTMMPLAGAAVLTLKPREIARGSTLISVTQQVGGSIGTALMATILTNQF 455 T ++ +LK +E G +L++ T + G A++ +L+ Sbjct: 362 LSFTKTVISTIVSSSLKQQEAGAGMSLLNFTSFLSEGTGIAIVGGLLSIPL 412
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_0507	IGASERPTASE	30	0.011	IgA-specific serine endopeptidase (S6) signature.
>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature. Length = 1541 Score = 29.6 bits (66), Expect = 0.011 Identities = 17/51 (33%), Positives = 20/51 (39%), Gaps = 3/51 (5%) Query: 143 VTFDEDRHRAHTGNGAQVLATLRNTAINLHRLNGADNIAEACRITALTANR 193 V E+ H TGN L N I+ LN ADN + LT N Sbjct: 846 VRLTENSHWHLTGNSDVHQLDLANGHIH---LNSADNSNNVTKYNTLTVNS 893
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_0518	PREPILNPTASE	29	0.013	Type IV prepilin cysteine protease (C20) family sig...
>PREPILNPTASE#Type IV prepilin cysteine protease (C20) family signature. Length = 290 Score = 29.4 bits (66), Expect = 0.013 Identities = 18/58 (31%), Positives = 23/58 (39%), Gaps = 11/58 (18%) Query: 1 MMGIVAPPLAVFFPKAAVVVGAVAGVWIL-LSRTIFKAFGGK----------LAAQGA 47 G++ L F V+GA+AG +L FK GK LAA GA Sbjct: 167 WGGLLFNLLGGFVSLGDAVIGAMAGYLVLWSLYWAFKLLTGKEGMGYGDFKLLAALGA 224
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_0521	IGASERPTASE	30	0.019	IgA-specific serine endopeptidase (S6) signature.
>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature. Length = 1541 Score = 30.0 bits (67), Expect = 0.019 Identities = 17/51 (33%), Positives = 20/51 (39%), Gaps = 3/51 (5%) Query: 283 VTFDEDRHRAHTGNGAQVLATLRNTAINLHRLNGADNIAEACRITALTANR 333 V E+ H TGN L N I+ LN ADN + LT N Sbjct: 846 VRLTENSHWHLTGNSDVHQLDLANGHIH---LNSADNSNNVTKYNTLTVNS 893

MUL_0562

MUL_0596

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
MUL_0562	5	17	4.430730	hypothetical protein
MUL_0566	3	16	2.274428	non-IS element not present in Mycobacterium
MUL_0567	9	16	5.121098	hypothetical protein
MUL_0568	6	15	4.172636	*transcriptional regulatory protein
MUL_0569	4	13	4.167258	hypothetical protein
MUL_0570	3	12	2.773269	hypothetical protein
MUL_0571	1	14	1.536556	deoxycytidine triphosphate deaminase
MUL_0572	1	12	2.085235	hypothetical protein
MUL_0573	3	12	0.959396	non-IS element not present in Mycobacterium
MUL_0574	3	12	0.794909	hypothetical protein
MUL_0575	1	15	0.594599	hypothetical protein
MUL_0576	1	13	1.712364	alpha-D-glucose-1-phosphate thymidylyl-
MUL_0578	2	14	2.283971	PE PGRS family protein
MUL_0579	2	14	2.562819	PE PGRS family protein
MUL_0580	0	11	1.105103	C-term transposase for IS2404
MUL_0581	1	13	2.054942	PE-PGRS family protein
MUL_0582	2	11	1.629389	non-IS element not present in Mycobacterium
MUL_0583	2	10	1.297982	non-IS element not present in Mycobacterium
MUL_0584	0	11	0.855137	aminotransferase AlaT
MUL_0585	0	11	0.490258	iron-sulfur-binding reductase
MUL_0587	1	11	0.754877	transposase for IS2606
MUL_0588	1	11	0.603221	C-term transposase for IS2404
MUL_0589	2	13	-0.291471	hypothetical protein
MUL_0593	1	13	-1.227130	membrane protein, IniB
MUL_0594	0	17	-2.143797	hypothetical protein
MUL_0595	-1	18	-2.438119	protein IniC
MUL_0596	-1	17	-3.099911	hypothetical protein

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_0569	cloacin	40	8e-06	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 40.5 bits (94), Expect = 8e-06 Identities = 35/114 (30%), Positives = 46/114 (40%), Gaps = 5/114 (4%) Query: 230 GGNGGQGGTGGTLFGNGGGGGTGGAGFVGPSSAGDGGNGGGGGRAGLIGNGGAGGAGGAP 289 G N G T G + G G G GG G + + GGG +G+ GG+G Sbjct: 8 GHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGI---HWGGGSGHGN 64 Query: 290 GPNGGFSGGNGGNGGDAVLIGNGGNSGDVGLS--GAGTPGLPGNGGLLIDTIGN 341 G G SGG G GG+ + G LS GAG + + G L I + Sbjct: 65 GGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAAIAD 118 Score = 35.1 bits (80), Expect = 4e-04 Identities = 38/106 (35%), Positives = 44/106 (41%), Gaps = 11/106 (10%) Query: 201 GGDGRLFGNGGAGGVGGAAYTSNILMNATGGNGGQGGTGGTLFG-----NGGGGGTGGAG 255 GGDGR N GA G NI TG G G + G+ + GGG G+G Sbjct: 3 GGDGRGH-NTGAHSTSG-----NINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHW 56 Query: 256 FVGPSSAGDGGNGGGGGRAGLIGNGGAGGAGGAPGPNGGFSGGNGG 301 G GGNG GG +G GN A A A G + G GG Sbjct: 57 GGGSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG 102 Score = 29.7 bits (66), Expect = 0.021 Identities = 30/87 (34%), Positives = 38/87 (43%), Gaps = 8/87 (9%) Query: 147 GLEQQGGTGGAAGLFGNGGDGGATGIFGGTGGAGGAGGQSTNALADSVGGNGGQGGDGRL 206 G + +G GA GN +GG TG+ G G + G+G S N G+G G G Sbjct: 3 GGDGRGHNTGAHSTSGNI-NGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 207 FGNGGAGGVGGAAYTSNILMNATGGNG 233 GNGG G G + TGGN Sbjct: 62 HGNGGGNGNSGGG-------SGTGGNL 81 Score = 29.3 bits (65), Expect = 0.027 Identities = 36/119 (30%), Positives = 41/119 (34%), Gaps = 12/119 (10%) Query: 181 GAGGQSTNALADSVGGNGGQGGDGRLFGNGGAGGVGGAAYTSNILMNATGGNGGQGGTGG 240 G G+ N A S GN GG L GGA G + +N +G GG Sbjct: 3 GGDGRGHNTGAHSTSGNI-NGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGG--- 58 Query: 241 TLFGNGGGGGTGGAGFVGPSSAGDGGNGGGGGRAGLIGNG-GAGGAGGAPGPNGGFSGG 298 G G G GG G S G G G A + G A GA G S G Sbjct: 59 -----GSGHGNGGGN--GNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAG 110
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_0570	cloacin	37	1e-04	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 37.0 bits (85), Expect = 1e-04 Identities = 36/110 (32%), Positives = 46/110 (41%), Gaps = 2/110 (1%) Query: 223 GAGGDGGVGGFGTFAGNGGDGGTGL-FAAGGDSGAGGDSVGGADGGDGGNGGKAGLVGQG 281 G G G G + +GN G TGL G G+G S GG G+G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 282 GDGGAGGNNNSGFGTGGSGGKGGDAVLIGNGGNGGNAGSGGVGPGIAGAA 331 G+GG GN+ G GTGG+ V G G+GG+ I+ A Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPAL-STPGAGGLAVSISAGA 111 Score = 35.5 bits (81), Expect = 3e-04 Identities = 27/80 (33%), Positives = 30/80 (37%), Gaps = 5/80 (6%) Query: 194 GRGGAGGAGGFGNNTTGGIGGLGGAGGLFGAGGDGGVGGFGTFAGNGGDGGTGLFAAGGD 253 GRG GA N GG GLG GG G G GG G+G+ GG Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGG-----ASDGSGWSSENNPWGGGSGSGIHWGGGS 60 Query: 254 SGAGGDSVGGADGGDGGNGG 273 G G + GG G G Sbjct: 61 GHGNGGGNGNSGGGSGTGGN 80 Score = 34.3 bits (78), Expect = 7e-04 Identities = 33/112 (29%), Positives = 41/112 (36%), Gaps = 13/112 (11%) Query: 243 GGTGLFAAGGDSGAGGDSVGGADGGDGGNGGKAGLVGQGGDGGAGGNNNSGFGTGGSGGK 302 GG G G G+ GG G G G G + GG + SG GG G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 303 GGDAVLIGNGGNGGNAGSGGVGPGIAGAA----------GIGGLLIGEDGMA 344 G GNG +GG +G+GG +A G GGL + A Sbjct: 63 GNGG---GNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGA 111 Score = 32.0 bits (72), Expect = 0.004 Identities = 25/81 (30%), Positives = 34/81 (41%), Gaps = 1/81 (1%) Query: 256 AGGDSVGGADGGDGGNGGKAGLVGQGGDGGAGGNNNSGFGTGGSGGKGGDAVLIGNGGNG 315 +GGD G G +G G G GG G ++ SG+ + + GG I GG Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGG-GASDGSGWSSENNPWGGGSGSGIHWGGGS 60 Query: 316 GNAGSGGVGPGIAGAAGIGGL 336 G+ GG G G+ G L Sbjct: 61 GHGNGGGNGNSGGGSGTGGNL 81 Score = 32.0 bits (72), Expect = 0.004 Identities = 28/81 (34%), Positives = 35/81 (43%), Gaps = 4/81 (4%) Query: 132 GAGGSGTPGTASVAGGNGGNGGAGGLLFGTGGAGGAG-GTASSLVGAIPGGNGGYGGDGG 190 G G G A GN NGG GL G G + G+G + ++ G G +GG G Sbjct: 3 GGDGRGHNTGAHSTSGNI-NGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 191 LLFGRGGAGGAGGFGNNTTGG 211 G GG G G G+ T G Sbjct: 62 --HGNGGGNGNSGGGSGTGGN 80 Score = 31.6 bits (71), Expect = 0.004 Identities = 34/108 (31%), Positives = 38/108 (35%), Gaps = 13/108 (12%) Query: 109 DTGRPLIGNGANGGAAGWLIGNGGAGGSGTPGTASVAGGNGGNGGAGGLLFGTGGAGGAG 168 +TG NGG G G G S G +S GG G+G G G G G Sbjct: 10 NTGAHSTSGNINGGPTG---LGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGG 66 Query: 169 GTASSLVGAIPGGNGGYGGDGGLLFGRGGAGGAGGFGNNTTGGIGGLG 216 G GN G G G A A GF +T G GGL Sbjct: 67 G----------NGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 30.5 bits (68), Expect = 0.010 Identities = 32/109 (29%), Positives = 46/109 (42%), Gaps = 9/109 (8%) Query: 238 GNGGDGGTGLFAAGGDSGAGGDSVGGADGGDGGNGGKAGLVGQGGDGGAGGNNNSGFGTG 297 G+G TG + G+ G +G G G+G + GG G+G + G G Sbjct: 4 GDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG-H 62 Query: 298 GSGGKGGDAVLIGNGGNGGNAGSGGVGPGIAGAAGIGGLLIGEDGMAGL 346 G+GG GNG +GG +G+GG +A G + G GL Sbjct: 63 GNGG--------GNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGL 103 Score = 29.7 bits (66), Expect = 0.020 Identities = 29/85 (34%), Positives = 29/85 (34%), Gaps = 7/85 (8%) Query: 162 GGAGGAGGTASSLVGAIPGGNGGYGGDGGLLFGRGGAGGAGGFGNNTTGGIGGLGGAGGL 221 G G A S G I GG G G GG G NN GG G G Sbjct: 4 GDGRGHNTGAHSTSGNINGGPTGLGVGGG-----ASDGSGWSSENNPWGGGSGSGI--HW 56 Query: 222 FGAGGDGGVGGFGTFAGNGGDGGTG 246 G G G GG G G G GG Sbjct: 57 GGGSGHGNGGGNGNSGGGSGTGGNL 81
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_0571	IGASERPTASE	30	0.016	IgA-specific serine endopeptidase (S6) signature.
>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature. Length = 1541 Score = 29.6 bits (66), Expect = 0.016 Identities = 17/51 (33%), Positives = 20/51 (39%), Gaps = 3/51 (5%) Query: 232 VTFDEDRHRAHTGNGAQVLATLRNTAINLHRLNGADNIAEACRITALTANR 282 V E+ H TGN L N I+ LN ADN + LT N Sbjct: 846 VRLTENSHWHLTGNSDVHQLDLANGHIH---LNSADNSNNVTKYNTLTVNS 893
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_0572	cloacin	41	1e-05	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 41.2 bits (96), Expect = 1e-05 Identities = 38/113 (33%), Positives = 46/113 (40%), Gaps = 1/113 (0%) Query: 684 GGAGGDGGSGGTGRGGTGGGGTGGGGTGGGGGVGINNGSGEAIGGAPGAGGTGAVGGDGG 743 G G + G GG G G GGG + G G NN G G GG G GG Sbjct: 8 GHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGG 67 Query: 744 QGGAAYSYGTGDATGSAGAAGTAGTTGVGGTGGAGGAAYTLNGASTATATGGI 796 G + GTG SA AA A T GAGG A +++ + + A I Sbjct: 68 NGNSGGGSGTG-GNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAAIADI 119 Score = 41.2 bits (96), Expect = 2e-05 Identities = 35/104 (33%), Positives = 45/104 (43%), Gaps = 5/104 (4%) Query: 434 GNGGTGGDSGAMGSSGG-RGGDGGVGTNGGAGGGGGNATSYGTANATGGAGGDGGTGSTG 492 G G G ++GA +SG GG G+G GGA G G + + N G G G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSG----WSSENNPWGGGSGSGIHWGG 58 Query: 493 NGGSGGDGGNGGTGHGGGGGPGGTAINYGAGDAFGGAAGKGGTG 536 G G GGNG +G G G G +A+ F + G G Sbjct: 59 GSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG 102 Score = 40.1 bits (93), Expect = 4e-05 Identities = 30/86 (34%), Positives = 37/86 (43%), Gaps = 3/86 (3%) Query: 360 GAGAVAGASGAAGTIIAGNGGNGGAGGAGYAADGPAGPAIGNGGDGGHGGAGGFYGNGGA 419 G G GA +G I NGG G G G A+DG + N GG G + G G Sbjct: 6 GRGHNTGAHSTSGNI---NGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 420 GGAGGNSAPGGGTGGNGGTGGDSGAM 445 G GGN GGG+G G + + Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPV 88 Score = 34.3 bits (78), Expect = 0.002 Identities = 36/124 (29%), Positives = 44/124 (35%), Gaps = 1/124 (0%) Query: 150 GSGAAGQAGGAGGAAGLIGTGGAGGMGGAGGGAGGMGGSGGWLLGNGGAGGAGGVGCAGV 209 G G GA +G I GG G+G GG + G G S GG+G G Sbjct: 3 GGDGRGHNTGAHSTSGNI-NGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 210 SGGVGGTGGNAMLFGNGGAGGMGGAGADGAVGAAGTAGTSTSAGGVGGAGWDATAAGVLA 269 G GG G + G GG A A T G A + A A ++A Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAAIADIMA 121 Query: 270 ATGG 273 A G Sbjct: 122 ALKG 125 Score = 33.9 bits (77), Expect = 0.003 Identities = 25/78 (32%), Positives = 30/78 (38%) Query: 429 GGGTGGNGGTGGDSGAMGSSGGRGGDGGVGTNGGAGGGGGNATSYGTANATGGAGGDGGT 488 G G G N G SG + G GG ++G N G+ + GG G Sbjct: 4 GDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHG 63 Query: 489 GSTGNGGSGGDGGNGGTG 506 GNG SGG G GG Sbjct: 64 NGGGNGNSGGGSGTGGNL 81 Score = 33.5 bits (76), Expect = 0.004 Identities = 31/105 (29%), Positives = 38/105 (36%), Gaps = 2/105 (1%) Query: 632 SGGGADINNGAFTVVPQGGAGGHGGDGATDGGAGGAGGFTEIDSSASVIAATGGAGGDGG 691 SGG +N GG G G G + G+G +E + + GG G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 692 SGGTGRGGTGGGGTGGGGTGGGGGVGINNGSGEAIGGAPGAGGTG 736 G G G GGG+G G G V G PGAGG Sbjct: 62 HGNGGGNGNSGGGSGTG--GNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 32.8 bits (74), Expect = 0.007 Identities = 32/115 (27%), Positives = 38/115 (33%), Gaps = 7/115 (6%) Query: 224 GNGGAGGMGGAGADGAVGAAGTAGTSTSAGGVGGAGWDATAAGVLAATGGDGGDSGGGGA 283 G G G GA + G G G G+GW + +G GG G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 284 GGNGGAGGAGGHGSALFGADGANGNGGAGGAGGNPGAPGNGGTGGVGPDAATSGG 338 G GG G +GG G GN A A G P G G + S G Sbjct: 63 GNGGGNGNSGGG-------SGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAG 110 Score = 32.4 bits (73), Expect = 0.009 Identities = 32/108 (29%), Positives = 37/108 (34%), Gaps = 5/108 (4%) Query: 278 SGGGGAGGNGGAGGAGGHGSALFGADGANGNGGAGGAGGNPGAPGNGGTGGVGPDAATSG 337 SGG G G N GA G+ + G G G GGA G G G + Sbjct: 2 SGGDGRGHNTGAHSTSGNING-----GPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHW 56 Query: 338 GMGGTGGDPGAVGGRGNSGAAGGAGAVAGASGAAGTIIAGNGGNGGAG 385 G G G+ G G G GG + A A G G GG Sbjct: 57 GGGSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 32.0 bits (72), Expect = 0.011 Identities = 27/89 (30%), Positives = 37/89 (41%), Gaps = 6/89 (6%) Query: 384 AGGAGYAADGPAGPAIGNGGDGGHGGAGGFYGNGGAGGAGGNSAPGGGTGGNGGTGGDSG 443 +GG G + A GN G G G + G+G + N+ GGG+G GG Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGG--- 58 Query: 444 AMGSSGGRGGDGGVGTNGGAGGGGGNATS 472 SG G G + GG+G GG + Sbjct: 59 ---GSGHGNGGGNGNSGGGSGTGGNLSAV 84 Score = 30.1 bits (67), Expect = 0.042 Identities = 22/79 (27%), Positives = 30/79 (37%) Query: 752 GTGDATGSAGAAGTAGTTGVGGTGGAGGAAYTLNGASTATATGGIGGNGGDGGTANGSNG 811 G G TG+ +G G G G + + + GG G GG + NG Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNG 65 Query: 812 GNGGAGGYASTTGTGTASV 830 G G G S TG ++V Sbjct: 66 GGNGNSGGGSGTGGNLSAV 84 Score = 30.1 bits (67), Expect = 0.049 Identities = 30/89 (33%), Positives = 37/89 (41%), Gaps = 5/89 (5%) Query: 481 GAGGDGGTGSTGNGGSGGDGGNGGTGHGGGGGPGGTAINYGAGDAFGGAAGKGGTGVVGG 540 G G + G ST +GG G G G G G G ++ N G G GG G Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGG-GASDGSGWSSENNPWGGGSGSGIHWGGGSG-HG 63 Query: 541 NGGSGGAAYNYGTGNATGAGGSSGSGGAA 569 NGG G N G G+ TG S+ + A Sbjct: 64 NGGGNG---NSGGGSGTGGNLSAVAAPVA 89
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_0574	IGASERPTASE	57	3e-10	IgA-specific serine endopeptidase (S6) signature.
>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature. Length = 1541 Score = 57.0 bits (137), Expect = 3e-10 Identities = 47/248 (18%), Positives = 68/248 (27%), Gaps = 12/248 (4%) Query: 726 LDREKATLPEKGTAAKEAEKRAKTAPKAAAPAAPAPAPAEA-PAKAAEASAAATAASPAA 784 +D T P A + + A AP P PA A P++ E A + Sbjct: 992 VDTTNITTPNNIQADVPSV-PSNNEEIARVDEAPVPPPAPATPSETTETVAENSKQESKT 1050 Query: 785 PAKGLGMAGGAK---RPGAKKAAPAPAAETAAAEAPAAPAKGLGMAAGAKKPGAKKAAAP 841 K A R AK+A A T E A+ + K+ A Sbjct: 1051 VEKNEQDATETTAQNREVAKEAKSNVKANTQTNEV----AQSGSETKETQTTETKETATV 1106 Query: 842 TGETKPAEAAAPAAPVKGLGMASGAKRPGAKKAAPPAAAAPEAAATAPAPEAAA---APA 898 E K V + K+ ++ P A A E T E + A Sbjct: 1107 EKEEKAKVETEKTQEVPKVTSQVSPKQEQSETVQPQAEPARENDPTVNIKEPQSQTNTTA 1166 Query: 899 EPAAPAAPVKGLGIATGAKRPGAKKAPARAEAPSAAAPAQPEPEATPEPEPASKQDGEPT 958 + PA + + E P PA +P E K + Sbjct: 1167 DTEQPAKETSSNVEQPVTESTTVNTGNSVVENPENTTPATTQPTVNSESSNKPKNRHRRS 1226 Query: 959 PPAAPAAP 966 + P Sbjct: 1227 VRSVPHNV 1234 Score = 42.4 bits (99), Expect = 9e-06 Identities = 35/199 (17%), Positives = 60/199 (30%), Gaps = 15/199 (7%) Query: 698 TDGVNDRQEEAGRSGVEV----LDVAQVLLGSLDREKATLPEKGTAAKEAEKRAK--TAP 751 T N + +S V+ +VAQ GS +E T K TA E E++AK T Sbjct: 1061 TTAQNREVAKEAKSNVKANTQTNEVAQS--GSETKETQTTETKETATVEKEEKAKVETEK 1118 Query: 752 KAAAPAAPAPAPAEAPAKAAEASAAATAASPAAPAKGLGMAGGAKRPGAKKAAPAPAAET 811 P ++ K ++ A PA + A A+ Sbjct: 1119 TQEVPK----VTSQVSPKQEQSETVQPQAEPARENDPTVNIKEPQSQTNTTADTEQPAKE 1174 Query: 812 AAAEAPAAPAKGLGMAAGAKKPGAKKAAAPTGETKPAEAAAPAAPVKGLGMASGAKRPGA 871 ++ + + G + P T+P + + K S R Sbjct: 1175 TSSNVEQPVTESTTVNTGNSVVENPENTTPA-TTQPTVNSESSNKPKNRHRRS--VRSVP 1231 Query: 872 KKAAPPAAAAPEAAATAPA 890 P ++ + + A Sbjct: 1232 HNVEPATTSSNDRSTVALC 1250
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_0579	PF03544	39	5e-05	Gram-negative bacterial tonB protein
>PF03544#Gram-negative bacterial tonB protein Length = 243 Score = 39.2 bits (91), Expect = 5e-05 Identities = 21/75 (28%), Positives = 22/75 (29%), Gaps = 10/75 (13%) Query: 878 NPPVINAPAPHVATPTPAHTPPVEHAPVVTPQPHVPAEQPAHHEPPSPSVFGHEPPVTHT 937 P + P P P P VE P P P P E P E P P P Sbjct: 56 APADL--EPPQAVQPPPE--PVVEPEPEPEPIPEPPKEAPVVIEKPKP------KPKPKP 105 Query: 938 PPVHVDPPSHGPVDP 952 PV V P Sbjct: 106 KPVKKVEQPKRDVKP 120
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_0582	SHAPEPROTEIN	38	9e-05	Bacterial cell shape determinant MreB/Mbl protein s...
>SHAPEPROTEIN#Bacterial cell shape determinant MreB/Mbl protein signature. Length = 347 Score = 37.8 bits (88), Expect = 9e-05 Identities = 55/245 (22%), Positives = 86/245 (35%), Gaps = 56/245 (22%) Query: 112 SGAQLGALRGALLTEPALAPNGVAAAVVSDAAAALAALRPTPGFPASGVVALCDFGAGGT 171 S GA L+ EP +AAA+ A L P A+G + + D G G T Sbjct: 129 SAQGAGAREVFLIEEP------MAAAI----GAGL------PVSEATGSM-VVDIGGGTT 171 Query: 172 SVTLAQVG----SSLQQIGPTFRYREFSGDEIDQLILNHI---LTVTPGIDSAEVSGTAT 224 V + + SS +IG GD D+ I+N++ G +AE Sbjct: 172 EVAVISLNGVVYSSSVRIG---------GDRFDEAIINYVRRNYGSLIGEATAE------ 216 Query: 225 SMGSVTLLLGGCRFAKEHLSA-APVATIATGAAGQPGADIRFSRNEFEQLITQPLDRFIG 283 + +G E +A G + NE + + +PL + Sbjct: 217 ---RIKHEIGSAYPGDEVREIEVRGRNLAEGVP----RGFTLNSNEILEALQEPLTGIVS 269 Query: 284 SVEDMLQRSGVPRPSLAA------VAAVGGGAAIPLIGNRLSERLQVPVFTTAQPIFSAA 337 +V L++ P LA+ + GGGA + + L E +PV P+ A Sbjct: 270 AVMVALEQC---PPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVA 326 Query: 338 IGAAM 342 G Sbjct: 327 RGGGK 331
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_0583	PYOCINKILLER	28	0.025	Pyocin S killer protein signature.
>PYOCINKILLER#Pyocin S killer protein signature. Length = 617 Score = 27.8 bits (61), Expect = 0.025 Identities = 18/99 (18%), Positives = 29/99 (29%) Query: 73 PTPASVTKTVTATMTTTTPTTTTAPTKTTTTTTTTTTTTTTTTTTTTTTTTTTTPTTTTT 132 + V+ + TT + T +TT T T + P++TT Sbjct: 373 VSTDGVSVPKAVPVRMAAYNATTGLYEVTVPSTTAEAPPLILTWTPASPPGNQNPSSTTP 432 Query: 133 TTTAPTTTTTTTTNPMSPGAMPTFPSQLTPSIPTVINLP 171 P T T+P +T +I P Sbjct: 433 VVPKPVPVYEGATLTPVKATPETYPGVITLPEDLIIGFP 471
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_0585	PF05616	31	0.003	Neisseria meningitidis TspB protein
>PF05616#Neisseria meningitidis TspB protein Length = 501 Score = 30.9 bits (69), Expect = 0.003 Identities = 23/87 (26%), Positives = 33/87 (37%), Gaps = 8/87 (9%) Query: 32 PIATPGAGPTEPSFPTRRPTTSPPTSTSP-SQPTSPASPTSPAGAIPLPPDDNGYVFIET 90 P PG P P P +P T P ++P SPA P P G + E Sbjct: 343 PNENPGTRPNPEPDPDLNPDANPDTDGQPGTRPDSPAVPDRPNGR-------HRKERKEG 395 Query: 91 KSGQTRCQINHDSVGCEAPFTNSPIKD 117 + G C+ D + C+ +P +D Sbjct: 396 EDGGLLCKFFPDILACDRLPEPNPAED 422
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_0593	SHAPEPROTEIN	136	2e-37	Bacterial cell shape determinant MreB/Mbl protein s...
>SHAPEPROTEIN#Bacterial cell shape determinant MreB/Mbl protein signature. Length = 347 Score = 136 bits (344), Expect = 2e-37 Identities = 73/368 (19%), Positives = 141/368 (38%), Gaps = 66/368 (17%) Query: 2 ARAVGIDLGTTNSVVAVLEGGDP-----VVVANSEGSRTTPSVVAFARNGEVLVGQPAKN 56 + + IDLGT N+++ V G VV + + + SV A VG AK Sbjct: 10 SNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQDRAGSPKSVAA--------VGHDAKQ 61 Query: 57 QAVTNVE--RTMRSVKRHMGGDWSIEIDDKKYTTPEISARVLMKLKRDAEAYLGEDIADA 114 +R +K + D+ + T ++ + ++ ++ Sbjct: 62 MLGRTPGNIAAIRPMKDGVIADF--------FVTEKMLQHFIKQVHSNS---FMRPSPRV 110 Query: 115 VITVPAYFNDAQRQATKDAGQIAGLNVLRIVNEPTAAALAYGLDKGEKDQTILVFDLGGG 174 ++ VP +R+A +++ Q AG + ++ EP AAA+ GL + +V D+GGG Sbjct: 111 LVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPV-SEATGSMVVDIGGG 169 Query: 175 TFDVSLLEIGEGVVEVRATSGDNHLGGDDWDDRVVEWLVDKFKGTSGIDLTKDKMAMQRL 234 T +V+++ + V S +GGD +D+ ++ ++ + G Sbjct: 170 TTEVAVISLNGVV-----YSSSVRIGGDRFDEAIINYVRRNYGSLIG------------- 211 Query: 235 REAAEKAKIELSSS----QSTSINLPYITVDAD--KNPLFLDEQLTRAEFQRITQDL--- 285 AE+ K E+ S+ + I + + + ++ A + +T + Sbjct: 212 EATAERIKHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAV 271 Query: 286 ---LDRTRKPFQSVIADTGISVSDIDHVVLVGGSTRMPAVTELVKELTGGKEPNKGVNPD 342 L++ S I++ G+ VL GG + + L+ E T G +P Sbjct: 272 MVALEQCPPELASDISERGM--------VLTGGGALLRNLDRLLMEET-GIPVVVAEDPL 322 Query: 343 EVVAVGAA 350 VA G Sbjct: 323 TCVARGGG 330

MUL_0630

MUL_0638

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
MUL_0630	3	14	2.117027	hypothetical protein
MUL_0631	2	13	1.883411	metal cation-transporting p-type ATPase F, CtpF
MUL_0632	0	14	2.420965	transposase for IS2404
MUL_0633	1	16	3.135783	ketopantoate reductase, ApbA
MUL_0634	1	14	3.118056	hypothetical protein
MUL_0635	-1	14	3.337846	hypothetical protein
MUL_0636	-1	13	3.783825	glutamate-1-semialdehyde aminotransferase
MUL_0637	1	13	4.237064	hypothetical protein
MUL_0638	1	12	3.426183	thioredoxin protein

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_0635	NUCEPIMERASE	174	6e-54	Nucleotide sugar epimerase signature.
>NUCEPIMERASE#Nucleotide sugar epimerase signature. Length = 334 Score = 174 bits (443), Expect = 6e-54 Identities = 93/367 (25%), Positives = 132/367 (35%), Gaps = 70/367 (19%) Query: 1 MKVLLTGAAGFIGSRVGAALSAAGHEVVGVDVLLPAAHGPNPVLPPG-----------CH 49 MK L+TGAAGFIG V L AGH+VVG+D L + L H Sbjct: 1 MKYLVTGAAGFIGFHVSKRLLEAGHQVVGIDNLND---YYDVSLKQARLELLAQPGFQFH 57 Query: 50 RVDVRDADAMAPLLA--GVDLVCHQAAMVGAGVDAADAPAYGGHNDLATTVLLAQMFAAG 107 ++D+ D + M L A + V + + AY N +L Sbjct: 58 KIDLADREGMTDLFASGHFERVFISPHRLAVRYSLENPHAYADSNLTGFLNILEGCRHNK 117 Query: 108 VRRLVLASSMVVYGQGHYDCPEHGRIDPLPRRHSDLDAGVFEHRCPLCAEPVRWRLVGEE 167 ++ L+ ASS VYG +P D V Sbjct: 118 IQHLLYASSSSVYGLNR----------KMPFSTDD---------------SVD------- 145 Query: 168 AELRPRSLYAASKTAQEHYALAWSESTGGSVVALRYHNVYGPGMPRDTPYSGVAAIFRSS 227 P SLYAA+K A E A +S G LR+ VYGP D F + Sbjct: 146 ---HPVSLYAATKKANELMAHTYSHLYGLPATGLRFFTVYGPWGRPDMALF----KFTKA 198 Query: 228 LEKGEPPKVFEDGGQMRDFVHVDDIAAANLAAAQL---------GEVDRHGFVAA----- 273 + +G+ V+ G RDF ++DDIA A + + E A Sbjct: 199 MLEGKSIDVYNYGKMKRDFTYIDDIAEAIIRLQDVIPHADTQWTVETGTPAASIAPYRVY 258 Query: 274 NVCSGRPISILQVASALCQARGDALSPVITGQYRSGDVRHIVADPSRAAEVLGFRAAVEP 333 N+ + P+ ++ AL A G + + GDV AD EV+GF Sbjct: 259 NIGNSSPVELMDYIQALEDALGIEAKKNM-LPLQPGDVLETSADTKALYEVIGFTPETTV 317 Query: 334 LEGLRGF 340 +G++ F Sbjct: 318 KDGVKNF 324
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_0637	cloacin	39	4e-05	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 39.3 bits (91), Expect = 4e-05 Identities = 24/53 (45%), Positives = 27/53 (50%), Gaps = 7/53 (13%) Query: 569 GGSSGGSSGGGSSS-----GGGSSSG--GGSSSGRGSSSGGSSSGGHSSGGGS 614 G G S G G SS GGGS SG G SG G+ G +SGG S GG+ Sbjct: 28 GVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNGNSGGGSGTGGN 80 Score = 32.4 bits (73), Expect = 0.005 Identities = 15/39 (38%), Positives = 19/39 (48%) Query: 567 PSGGSSGGSSGGGSSSGGGSSSGGGSSSGRGSSSGGSSS 605 P GG SG G SG G+ G G+S G + G S+ Sbjct: 45 PWGGGSGSGIHWGGGSGHGNGGGNGNSGGGSGTGGNLSA 83 Score = 31.6 bits (71), Expect = 0.010 Identities = 14/40 (35%), Positives = 16/40 (40%) Query: 566 PPSGGSSGGSSGGGSSSGGGSSSGGGSSSGRGSSSGGSSS 605 P GGS G GG S G G S G G+ S+ Sbjct: 45 PWGGGSGSGIHWGGGSGHGNGGGNGNSGGGSGTGGNLSAV 84 Score = 30.8 bits (69), Expect = 0.020 Identities = 15/40 (37%), Positives = 20/40 (50%) Query: 568 SGGSSGGSSGGGSSSGGGSSSGGGSSSGRGSSSGGSSSGG 607 SG GG SG G+ G G+S GG + G S+ + G Sbjct: 52 SGIHWGGGSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFG 91

MUL_0778

MUL_0804

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
MUL_0778	2	14	-3.435383	elongation factor Tu
MUL_0780	1	13	-3.110870	hypothetical protein
MUL_0781	2	13	-2.423554	3-ketoacyl-ACP reductase
MUL_0782	1	12	-2.255099	ferredoxin reductase
MUL_0784	0	13	-1.630866	hypothetical protein
MUL_0785	1	13	-0.982098	transcriptional regulator
MUL_0787	1	14	-0.670071	hypothetical protein
MUL_0788	4	22	-0.295969	coenzyme PQQ synthesis protein E, PqqE
MUL_0789	4	24	-1.258351	L-lactate dehydrogenase (cytochrome) LldD1
MUL_0790	4	21	-0.287498	creatinine amidohydrolase
MUL_0791	3	22	0.293576	membrane glycosyl transferase
MUL_0792	2	22	0.055139	dehydrogenase
MUL_0793	3	21	0.133881	TetR family transcriptional regulator
MUL_0794	6	21	-0.197079	transposase for IS2606
MUL_0795	3	17	0.728646	transposase for IS2404
MUL_0796	0	15	-0.511283	PPE family protein
MUL_0797	0	13	-1.415500	C-term transposase for IS2404
MUL_0798	1	14	-1.772708	N-term transposase for IS2404
MUL_0799	0	13	-1.743909	lipase
MUL_0801	1	13	-1.901456	hypothetical protein
MUL_0803	0	14	-3.121133	30S ribosomal protein S10
MUL_0804	2	20	-3.211480	50S ribosomal protein L3

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_0778	HTHTETR	53	6e-11	TetR bacterial regulatory protein HTH signature.
>HTHTETR#TetR bacterial regulatory protein HTH signature. Length = 215 Score = 53.5 bits (128), Expect = 6e-11 Identities = 29/190 (15%), Positives = 59/190 (31%), Gaps = 17/190 (8%) Query: 10 DHERLLRAAAEFLGRRP--NATQDEIAAAIGVSRATLHRYFAGRAALIDALEQLAFGQMR 67 + +L A ++ + + EIA A GV+R ++ +F ++ L + +L+ + Sbjct: 12 TRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSEIWELSESNIG 71 Query: 68 EAL-----KSARWQEGSATEELRRLVAAC---ESVSGYLTLLYAYSQDSDTNELKQGW-- 117 E K E L ++ + E + +++ + + Q Sbjct: 72 ELELEYQAKFPGDPLSVLREILIHVLESTVTEERRRLLMEIIFHKCEFVGEMAVVQQAQR 131 Query: 118 ---LEIDSEIKELFLRGQRQGEFRPDLAAGWLTEAFYSLVSG--AGWSINTGRAAPRDFA 172 LE I++ DL +SG W + A Sbjct: 132 NLCLESYDRIEQTLKHCIEAKMLPADLMTRRAAIIMRGYISGLMENWLFAPQSFDLKKEA 191 Query: 173 PMITELLLHG 182 +LL Sbjct: 192 RDYVAILLEM 201
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_0781	IGASERPTASE	30	0.021	IgA-specific serine endopeptidase (S6) signature.
>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature. Length = 1541 Score = 29.6 bits (66), Expect = 0.021 Identities = 17/50 (34%), Positives = 20/50 (40%), Gaps = 3/50 (6%) Query: 283 VTFDEDRHRAHTGNGAQVLATLRNTAINLHRLNGADNIAEACRITALTAN 332 V E+ H TGN L N I+ LN ADN + LT N Sbjct: 846 VRLTENSHWHLTGNSDVHQLDLANGHIH---LNSADNSNNVTKYNTLTVN 892
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_0782	cloacin	33	0.003	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 32.8 bits (74), Expect = 0.003 Identities = 26/75 (34%), Positives = 34/75 (45%), Gaps = 5/75 (6%) Query: 220 SGNVGNANNGIGNLGSGNL--GNFNLGSGNLGSSNAGWANLGSNNIGGANSGGNNLGWGN 277 SG G +N + SGN+ G LG G S +GW++ NN G S G+ + WG Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSS--ENNPWGGGS-GSGIHWGG 58 Query: 278 LGGLNTGFANAGSGN 292 G G N SG Sbjct: 59 GSGHGNGGGNGNSGG 73 Score = 29.3 bits (65), Expect = 0.045 Identities = 24/94 (25%), Positives = 32/94 (34%), Gaps = 2/94 (2%) Query: 217 NIGSGNVGNANNGIGNLGSGNLGNFNLGSGNLGSSNAGWANLGSNNIGGANSGGNNLGWG 276 NI G G G + GSG N G GS G N GG + G G G Sbjct: 19 NINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNGNSGGGSGTG 78 Query: 277 NLGGLNTGFANAGSGNFRFANTGNNNIGIGLTGD 310 G L+ A G + G + + ++ Sbjct: 79 --GNLSAVAAPVAFGFPALSTPGAGGLAVSISAG 110

MUL_0877

MUL_0902

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
MUL_0877	1	14	3.269644	hypothetical protein
MUL_0878	1	14	2.529047	EsaT-6 like protein, EsxU
MUL_0879	0	13	3.625125	EsaT-6 like protein, EsxT
MUL_0880	0	15	3.483610	50S ribosomal protein L13
MUL_0881	0	16	3.026177	30S ribosomal protein S9
MUL_0882	-1	12	1.536176	phosphoglucosamine mutase
MUL_0883	-3	12	1.193436	hypothetical protein
MUL_0884	1	13	0.051854	hypothetical protein
MUL_0885	1	13	-0.769467	hypothetical protein
MUL_0886	1	10	-1.269383	glucosamine--fructose-6-phosphate
MUL_0888	2	12	-1.968740	transposase for IS2404
MUL_0889	2	12	-1.986303	hypothetical protein
MUL_0890	1	12	-2.541039	transmembrane protein
MUL_0891	0	15	-1.251999	glutamate decarboxylase
MUL_0894	-1	13	-1.241863	alanine racemase
MUL_0895	-1	11	0.410085	hydrolase
MUL_0896	-2	9	0.929047	hypothetical protein
MUL_0897	3	9	0.792869	hypothetical protein
MUL_0898	3	11	0.851737	ribosomal-protein-alanine acetyltransferase,
MUL_0899	3	12	0.285242	putative DNA-binding/iron metalloprotein/AP
MUL_0900	3	12	0.440175	co-chaperonin GroES
MUL_0901	2	13	0.434670	chaperonin GroEL
MUL_0902	3	10	-0.619018	transposase for IS2404

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_0879	ALARACEMASE	399	e-141	Alanine racemase signature.
>ALARACEMASE#Alanine racemase signature. Length = 356 Score = 399 bits (1026), Expect = e-141 Identities = 113/373 (30%), Positives = 177/373 (47%), Gaps = 28/373 (7%) Query: 14 AEAVVDLGAIAHNVRLLRERAGSAQVMAVVKADGYGHGATAVARTALAAGAVELGVASVD 73 +A +DL A+ N+ ++R+ A A+V +VVKA+ YGHG + A + +++ Sbjct: 5 IQASLDLQALKQNLSIVRQAATHARVWSVVKANAYGHGIERIWSAIGATDG--FALLNLE 62 Query: 74 EALTLRADGITAPVL---AWLHAPGMDFGPALAADVQIAISSIRQLDEALDAARRTGTTA 130 EA+TLR G P+L + HA ++ + + S QL +A R Sbjct: 63 EAITLRERGWKGPILMLEGFFHAQDLEIYDQ--HRLTTCVHSNWQLKALQNA--RLKAPL 118 Query: 131 TVTVKIDTGLNRNGVAPALYPEMVTRLRQAVAEDAIRLRGLMTHMVHADAPEKPINDIQS 190 + +K+++G+NR G P ++T +Q A + LM+H A+ P+ + Sbjct: 119 DIYLKVNSGMNRLGFQP---DRVLTVWQQLRAMANVGEMTLMSHFAEAEHPDGISGAMA- 174 Query: 191 QRFKQMLDHARDQGVRFEVAHLSNSSATMARPDLTLDLVRPGIAVYGLSPVPRLGDM--- 247 R +Q +G+ LSNS+AT+ P+ D VRPGI +YG SP + D+ Sbjct: 175 -RIEQAA-----EGLECRR-SLSNSAATLWHPEAHFDWVRPGIILYGASPSGQWRDIANT 227 Query: 248 GLVPAMTVKCAVALVKSVSAGEGVSYGHTWIAPHDTNVALLPIGYADGVFRSLGGRLEVL 307 GL P MT+ + V+++ AGE V YG + A + + ++ GYADG R VL Sbjct: 228 GLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGYPRHAPTGTPVL 287 Query: 308 INGKRRPGVGRVCMDQFLVDLGPGPLDVAEGDEAILFGPGTRGEPTAQDWADLVGTIHYE 367 ++G R VG V MD VDL P P G L+G E D A GT+ YE Sbjct: 288 VDGVRTMTVGTVSMDMLAVDLTPCP-QAGIGTPVELWGK----EIKIDDVAAAAGTVGYE 342 Query: 368 VVTSPRGRITRVY 380 ++ + R+ V Sbjct: 343 LMCALALRVPVVT 355
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_0883	SACTRNSFRASE	47	3e-09	Streptothricin acetyltransferase signature.
>SACTRNSFRASE#Streptothricin acetyltransferase signature. Length = 173 Score = 46.9 bits (111), Expect = 3e-09 Identities = 20/93 (21%), Positives = 32/93 (34%), Gaps = 8/93 (8%) Query: 53 GARCADNLVGYAGV-SRLGRVAPFEYEIHTIGVDPAYQGRGIGRRLLDELLAFA---DGG 108 +N +G + S A I I V Y+ +G+G LL + + +A Sbjct: 69 LYYLENNCIGRIKIRSNWNGYA----LIEDIAVAKDYRKKGVGTALLHKAIEWAKENHFC 124 Query: 109 VVFLEVRTDNEPAIALYRSVGFEQVGLRRRYYR 141 + LE + N A Y F + Y Sbjct: 125 GLMLETQDINISACHFYAKHHFIIGAVDTMLYS 157
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_0888	IGASERPTASE	30	0.019	IgA-specific serine endopeptidase (S6) signature.
>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature. Length = 1541 Score = 30.0 bits (67), Expect = 0.019 Identities = 17/51 (33%), Positives = 20/51 (39%), Gaps = 3/51 (5%) Query: 283 VTFDEDRHRAHTGNGAQVLATLRNTAINLHRLNGADNIAEACRITALTANR 333 V E+ H TGN L N I+ LN ADN + LT N Sbjct: 846 VRLTENSHWHLTGNSDVHQLDLANGHIH---LNSADNSNNVTKYNTLTVNS 893
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_0889	cloacin	27	0.042	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 26.6 bits (58), Expect = 0.042 Identities = 21/65 (32%), Positives = 24/65 (36%), Gaps = 6/65 (9%) Query: 79 GPGGWGGPGGPGGPPASGEPYGPYGPQGGAPGTPGWPGGIAGPTAAGGHAGMGGIGGMDG 138 GP G G GG P+G GG+ W GG + G G G GG G Sbjct: 23 GPTGLGVGGGASDGSGWSSENNPWG--GGSGSGIHWGGG----SGHGNGGGNGNSGGGSG 76 Query: 139 MGGMG 143 GG Sbjct: 77 TGGNL 81
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_0890	cloacin	35	8e-04	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 35.1 bits (80), Expect = 8e-04 Identities = 28/100 (28%), Positives = 38/100 (38%), Gaps = 6/100 (6%) Query: 187 NANLGSGNTGIGNIGVGNSGEGNSALVPPQSGNYNIGGGNNGNNNLGAGNIGNFNFGFGN 246 + N+ G TG+G G + G G S+ P G G G + G GN G G Sbjct: 17 SGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGS--GHGNGGGNGNSGGG 74 Query: 247 NGTGNFGFGNAGPADLSNPNLFTFHVTPGENNIGIGNTGN 286 +GTG A P P L TPG + + + Sbjct: 75 SGTGGNLSAVAAPVAFGFPAL----STPGAGGLAVSISAG 110 Score = 29.3 bits (65), Expect = 0.049 Identities = 22/81 (27%), Positives = 32/81 (39%), Gaps = 3/81 (3%) Query: 341 GSGNIGIGNSGSNNIGFFNSGDGNIGAFSSGTNSVFPGQLNSFGVGNSGTGNLGFGNAGS 400 G G+ +S S NI N G +G ++ N+ G SG+G G +G Sbjct: 6 GRGHNTGAHSTSGNI---NGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 401 GNAGFGNSGLLNTGFGNAGST 421 GN G + +G G S Sbjct: 63 GNGGGNGNSGGGSGTGGNLSA 83
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_0894	UREASE	35	8e-04	Urea amidohydrolase (urease) protein signature.
>UREASE#Urea amidohydrolase (urease) protein signature. Length = 570 Score = 35.1 bits (81), Expect = 8e-04 Identities = 24/76 (31%), Positives = 35/76 (46%), Gaps = 9/76 (11%) Query: 6 DAIYTNGDIVTVDDEQPIAEA-VAVKDGRIVAVGAHD-----DVVRENLGPHTRRVDLAG 59 D + TN I+ D I +A + +KDGRI A+G V +GP T + G Sbjct: 69 DTVITNALIL---DHWGIVKADIGLKDGRIAAIGKAGNPDMQPGVTIIVGPGTEVIAGEG 125 Query: 60 NTLLPGFIDPHSHYIN 75 + G +D H H+I Sbjct: 126 KIVTAGGMDSHIHFIC 141 Score = 31.2 bits (71), Expect = 0.013 Identities = 13/30 (43%), Positives = 17/30 (56%) Query: 487 ITINAAYQYSEEQSKGSITVGKLADLVIVD 516 TIN A + GS+ VGK ADLV+ + Sbjct: 409 YTINPAIAHGLSHEIGSLEVGKRADLVLWN 438
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_0896	IGASERPTASE	30	0.017	IgA-specific serine endopeptidase (S6) signature.
>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature. Length = 1541 Score = 30.0 bits (67), Expect = 0.017 Identities = 17/51 (33%), Positives = 20/51 (39%), Gaps = 3/51 (5%) Query: 283 VTFDEDRHRAHTGNGAQVLATLRNTAINLHRLNGADNIAEACRITALTANR 333 V E+ H TGN L N I+ LN ADN + LT N Sbjct: 846 VRLTENSHWHLTGNSDVHQLDLANGHIH---LNSADNSNNVTKYNTLTVNS 893
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_0898	PF03544	30	0.011	Gram-negative bacterial tonB protein
>PF03544#Gram-negative bacterial tonB protein Length = 243 Score = 29.9 bits (67), Expect = 0.011 Identities = 22/104 (21%), Positives = 29/104 (27%), Gaps = 4/104 (3%) Query: 246 PAPSASPTTTGAKPSASLPPAGATATSPAPTSVPTPPVSAVVPGETPADTSVVAPGSPAA 305 PAP+ + T P+ PP A P P V P E P + VV Sbjct: 44 PAPAQPISVTMVAPADLEPPQ---AVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPK 100 Query: 306 AGVAAPGPAKLAAP-GDATNPGSPVVQTSGQPEPVEPAPAGPVS 348 K+ P D S P P + + Sbjct: 101 PKPKPKPVKKVEQPKRDVKPVESRPASPFENTAPARPTSSTATA 144
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_0899	CHLAMIDIAOMP	27	0.027	Chlamydia major outer membrane protein signature.
>CHLAMIDIAOMP#Chlamydia major outer membrane protein signature. Length = 393 Score = 26.9 bits (59), Expect = 0.027 Identities = 12/31 (38%), Positives = 13/31 (41%), Gaps = 11/31 (35%) Query: 5 LPPGLPPDP-----------FADDPCDPSAT 24 LP G P +P F DPCDP T Sbjct: 23 LPVGNPAEPSLMIDGILWEGFGGDPCDPCTT 53
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_0902	cloacin	30	0.033	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 30.1 bits (67), Expect = 0.033 Identities = 28/119 (23%), Positives = 38/119 (31%), Gaps = 13/119 (10%) Query: 413 GFGNGGNFNLGFGNGGAANVGVGNGGGGNLGFGNSGTENTGSFNSGGDNGRNGGNTGSFN 472 G G + G NGG +GVG G G+ + G SG G G+ Sbjct: 8 GHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGG 67 Query: 473 SGYVNTGFFNSGSTNTGLFNAGSVNTGIGSPDTQPGSISGFGNTGAGVSGFNNSGDATS 531 +G NSG + N +V + GAG + S A S Sbjct: 68 NG-------NSGGGSGTGGNLSAVAAPVAF------GFPALSTPGAGGLAVSISAGALS 113 Score = 30.1 bits (67), Expect = 0.033 Identities = 27/81 (33%), Positives = 36/81 (44%), Gaps = 12/81 (14%) Query: 396 NSGDTNT-GFWNAGRVNTGFGNGGNFNLGFG-----NGGAANVGVGNGGGGNLGFGNSGT 449 N+G +T G N G G G G + G+ GG + G+ GGG G GN G Sbjct: 10 NTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGS--GHGNGG- 66 Query: 450 ENTGSFNSGGDNGRNGGNTGS 470 G+ NSGG +G G + Sbjct: 67 ---GNGNSGGGSGTGGNLSAV 84 Score = 29.7 bits (66), Expect = 0.036 Identities = 30/84 (35%), Positives = 35/84 (41%), Gaps = 6/84 (7%) Query: 190 NTGVGNLSIGNI--GVFNLGGGNAGNLNLGGGNTGNANLGSGNNGFFNLGSGNTGNTNFG 247 NTG + S GNI G LG G + G + N G +G G GN G Sbjct: 10 NTGAHSTS-GNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGG-G 67 Query: 248 NGNRGNLNWGSGNLGN--ANVGFG 269 NGN G + GNL A V FG Sbjct: 68 NGNSGGGSGTGGNLSAVAAPVAFG 91 Score = 29.7 bits (66), Expect = 0.043 Identities = 24/77 (31%), Positives = 31/77 (40%), Gaps = 1/77 (1%) Query: 227 GSGNNGFFNLGSGNTGNTNFGNGNRGNLNWGSGNLGNANVGFGNFLGQGNFGFGNRVGDA 286 G G+N + SGN G G G + GSG + N +G G G G Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGGASDGSG-WSSENNPWGGGSGSGIHWGGGSGHGN 64 Query: 287 NLGSGNLGNANFGNGNL 303 G+GN G + GNL Sbjct: 65 GGGNGNSGGGSGTGGNL 81

MUL_0958

MUL_1004

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
MUL_0958	3	13	-0.314582	PPE family protein
MUL_0959	7	13	-1.889745	PPE family protein
MUL_0960	6	13	-2.826622	hypothetical protein
MUL_0961	6	15	-3.153566	integral membrane transport protein
MUL_0962	5	13	-3.274145	transposase for IS2404
MUL_0964	5	11	-3.145262	hypothetical protein
MUL_0965	3	11	-3.241813	putative regulatory protein
MUL_0966	-1	15	-3.350417	transferase
MUL_0967	-1	15	-3.350417	succinyl-diaminopimelate desuccinylase
MUL_0971	0	15	-1.866745	hypothetical protein
MUL_0972	0	14	-1.705874	hypothetical protein
MUL_0974	0	16	-1.540650	long-chain-acyl-CoA synthetase
MUL_0976	1	14	-1.046262	dihydropteroate synthase 2 FolP2
MUL_0977	1	13	-0.185494	putative glucosyl-3-phosphoglycerate synthase
MUL_0979	2	11	1.215016	hypothetical protein
MUL_0980	1	13	0.300116	DNA-3-methyladenine glycosylase I TagA
MUL_0982	2	11	0.422076	hypothetical protein
MUL_0983	0	11	0.125430	PPE family protein
MUL_0985	0	12	0.313346	PPE family protein
MUL_0986	-1	14	0.260268	transposase for IS2606
MUL_0987	-1	14	-0.259408	PE family protein
MUL_0988	-1	14	-0.253420	acetyl-CoA acetyltransferase
MUL_0989	0	12	-0.391459	enoyl-CoA hydratase, EchA1
MUL_0990	1	15	-0.775833	transposase for IS2606
MUL_0991	2	14	-0.894139	transposase for IS2404
MUL_0992	2	15	-1.950110	transposase for IS2606
MUL_0993	0	17	-3.202801	oxidoreductase
MUL_0995	1	18	-4.185592	hypothetical protein
MUL_0996	3	32	-7.873770	hypothetical protein
MUL_0997	5	32	-7.602027	integral membrane protein
MUL_0998	4	33	-7.875985	enoyl-CoA hydratase
MUL_0999	4	28	-3.570067	alpha-methylacyl-CoA racemase Mcr
MUL_1000	6	24	-1.669179	short-chain type dehydrogenase/reductase
MUL_1001	5	21	-0.523738	hypothetical protein
MUL_1002	2	16	3.045835	transmembrane transport protein MmpL13
MUL_1003	2	15	3.835289	TetR family transcriptional regulator
MUL_1004	-1	12	3.246056	hypothetical protein

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_0964	cloacin	34	0.002	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 34.3 bits (78), Expect = 0.002 Identities = 24/81 (29%), Positives = 31/81 (38%), Gaps = 6/81 (7%) Query: 458 MGFGNGGGGNTGFY------NSGTYNTGFSNAAETNTGWENSGNVNTGGYNSGGLNTGIG 511 M G+G G NTG + N G G A +GW + N GG SG G Sbjct: 1 MSGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGS 60 Query: 512 SPDTQAGPNSGFGHSGSGNSG 532 G + G SG+G + Sbjct: 61 GHGNGGGNGNSGGGSGTGGNL 81 Score = 30.8 bits (69), Expect = 0.017 Identities = 23/89 (25%), Positives = 30/89 (33%), Gaps = 11/89 (12%) Query: 225 GSGNTGSANLGGGNIGNGNLGSGNTGNVNLGNGNNGFFNFGNGNLGDTNFGSGNSGNLNL 284 G G+ A+ GNI G G G G + G+G N G + Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGGASDGSG-----------WSSENNPWGGGSGSGI 54 Query: 285 GSGNRFGSGNIGFGNRFGDGNFGSGNAGS 313 G G GN G G G+ GN + Sbjct: 55 HWGGGSGHGNGGGNGNSGGGSGTGGNLSA 83 Score = 30.1 bits (67), Expect = 0.030 Identities = 27/87 (31%), Positives = 35/87 (40%), Gaps = 3/87 (3%) Query: 327 GSGNNGDSNIGFGNLGTNNLGFGNNGSNNIGFGLSGSNQIGIGGLNAGI---GNMGFGNA 383 G G+N ++ GN+ G G G + G G S N GG +GI G G GN Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNG 65 Query: 384 GDNNVGFFNSGSNNIGFFNSGDGNFGF 410 G N SG+ + FGF Sbjct: 66 GGNGNSGGGSGTGGNLSAVAAPVAFGF 92 Score = 30.1 bits (67), Expect = 0.034 Identities = 27/86 (31%), Positives = 31/86 (36%), Gaps = 9/86 (10%) Query: 406 GNFGFAHAGSTNTGFWNSGGTNTGFGNGGSLNFGFG-------NGGVENMGHGNAGSFNM 458 G G H ++ N G TG G GG + G G GG G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGG--GS 60 Query: 459 GFGNGGGGNTGFYNSGTYNTGFSNAA 484 G GNGGG SGT + AA Sbjct: 61 GHGNGGGNGNSGGGSGTGGNLSAVAA 86 Score = 29.7 bits (66), Expect = 0.038 Identities = 27/90 (30%), Positives = 38/90 (42%), Gaps = 10/90 (11%) Query: 378 MGFGNAGDNNVGFFNSGSNNIGFFNSGDGNFGFAHAGSTNTGFWNSGGTNTGFGNGGSLN 437 M G+ +N G ++ N N G G S +G W+S G G+G ++ Sbjct: 1 MSGGDGRGHNTGAHSTSGN----INGGPTGLGVGGGASDGSG-WSSENNPWGGGSGSGIH 55 Query: 438 FGFGNGGVENMGHGNAGSFNMGFGNGGGGN 467 +G G G N G N G G+G GGN Sbjct: 56 WG-GGSGHGNGGGNG----NSGGGSGTGGN 80
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_0965	cloacin	34	0.003	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 33.9 bits (77), Expect = 0.003 Identities = 28/109 (25%), Positives = 41/109 (37%), Gaps = 3/109 (2%) Query: 727 NSGSYNT-GSFNSGTLNTGDFNGGDHNTGWGNSGNTNTGGINSGDLNTGFGSSADQAVTN 785 N+G+++T G+ N G G G +GW + N GG SG G S Sbjct: 10 NTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGI--HWGGGSGHGNGGG 67 Query: 786 SGFGNNGSGNSGFNNTGDTNSGFHNANTSALFSGHSGLLNAGGSQSVGI 834 +G GSG G + F S +G + + G+ S I Sbjct: 68 NGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAAI 116
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_0979	SECA	33	0.003	SecA protein signature.
>SECA#SecA protein signature. Length = 901 Score = 32.5 bits (74), Expect = 0.003 Identities = 24/86 (27%), Positives = 32/86 (37%), Gaps = 15/86 (17%) Query: 243 VAGRVLLVGDAAGYEDALTGEGITLAVKQAAA-------AVRAIADND-PASYEAAWHRV 294 + G VL A + TGEG TL A V + ND A +A Sbjct: 89 LGGMVLNERCIA---EMRTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAEN--N 143 Query: 295 TRSYRWL--TRGLVLASAPRPARRAI 318 + +L T G+ L P PA+R Sbjct: 144 RPLFEFLGLTVGINLPGMPAPAKREA 169
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_0982	NUCEPIMERASE	63	3e-13	Nucleotide sugar epimerase signature.
>NUCEPIMERASE#Nucleotide sugar epimerase signature. Length = 334 Score = 63.3 bits (154), Expect = 3e-13 Identities = 31/125 (24%), Positives = 51/125 (40%), Gaps = 18/125 (14%) Query: 38 MRILVTGATGYVGSRLVTALLADGHEVLA---------ATRNMARLSRLAWFDDVTPVIL 88 M+ LVTGA G++G + LL GH+V+ + ARL LA + Sbjct: 1 MKYLVTGAAGFIGFHVSKRLLEAGHQVVGIDNLNDYYDVSLKQARLELLA-QPGFQFHKI 59 Query: 89 DATDRASAQAAMNAAGQIDVVYYLVH------GIGQPD-FRDRDKTAAANLAVAARDTGV 141 D DR + A+G + V+ H + P + D + T N+ R + Sbjct: 60 DLADRE-GMTDLFASGHFERVFISPHRLAVRYSLENPHAYADSNLTGFLNILEGCRHNKI 118 Query: 142 RRIVY 146 + ++Y Sbjct: 119 QHLLY 123
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_0987	DHBDHDRGNASE	72	3e-17	2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase signature. Length = 261 Score = 72.4 bits (177), Expect = 3e-17 Identities = 59/205 (28%), Positives = 88/205 (42%), Gaps = 19/205 (9%) Query: 3 IRDAVAVVTGGASGLGLATTKRLLDAGAQVVVLDIRGE---DVVADLGDRARFA---AAD 56 I +A +TG A G+G A + L GA + +D E VV+ L AR A AD Sbjct: 6 IEGKIAFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHAEAFPAD 65 Query: 57 VTDEAAVASALD-LAETMGTLRIVVNCAGTGNAIRVLSRDGVFSLAAFRKIVDINLVGSF 115 V D AA+ + MG + I+VN AG +R + S + +N G F Sbjct: 66 VRDSAAIDEITARIEREMGPIDILVNVAGV---LRPGLIHSL-SDEEWEATFSVNSTGVF 121 Query: 116 NVLRLAAERIAKTEPVGPNAEERGVIINTASVAAFDGQIGQAAYSASKGGVVGMTLPIAR 175 N R ++ + G I+ S A + AAY++SK V T + Sbjct: 122 NASRSVSKYM--------MDRRSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGL 173 Query: 176 DLASHRIRVMTIAPGLFDTPLLASL 200 +LA + IR ++PG +T + SL Sbjct: 174 ELAEYNIRCNIVSPGSTETDMQWSL 198
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_0989	ACRIFLAVINRP	54	2e-09	Acriflavin resistance protein family signature.
>ACRIFLAVINRP#Acriflavin resistance protein family signature. Length = 1034 Score = 53.7 bits (129), Expect = 2e-09 Identities = 36/233 (15%), Positives = 85/233 (36%), Gaps = 29/233 (12%) Query: 186 LIAIPLSFLVLVWVFGGLLAAALPMALGALAVVGSMSVLRLVTFTTDVSIFALNLSTALG 245 AI L FLV+ + A +P + ++G+ ++L ++ +N T G Sbjct: 345 FEAIMLVFLVMYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYS-------INTLTMFG 397 Query: 246 LALAI-----DYTLLIISRYRDELAEGSSREEALVRTMATSGRTVLFSAVT---VALSMS 297 + LAI D +++ + R + + +EA ++M+ ++ A+ V + M+ Sbjct: 398 MVLAIGLLVDDAIVVVENVERVMMEDKLPPKEATEKSMSQIQGALVGIAMVLSAVFIPMA 457 Query: 298 ATVAFPMYFLKSFAYAGVATVAFVATASIVVTPAAIVLLGPRLDALNVRRLARRMLGRPK 357 + F+ V+ +A ++++TPA L ++ ++ Sbjct: 458 FFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATL--------LKPVSAEHHENKG 509 Query: 358 PQHKPVDQLF------WYRSTKFVMRRALPVGLAVVAVLVILGLPFFSVKWGF 404 + F + S ++ L ++ + + F + F Sbjct: 510 GFFGWFNTTFDHSVNHYTNSVGKILGSTGRYLLIYALIVAGMVVLFLRLPSSF 562 Score = 40.6 bits (95), Expect = 3e-05 Identities = 42/233 (18%), Positives = 81/233 (34%), Gaps = 27/233 (11%) Query: 116 SAPDLVSKDGKSGL-IVVNIKGGES--NAQKNAQTLADEIVHDRDGVTVRAGGSAMEYAQ 172 +P L +G + I G S +A + LA ++ G+ G + + Sbjct: 811 GSPRLERYNGLPSMEIQGEAAPGTSSGDAMALMENLASKLP---AGIGYDWTGMSYQERL 867 Query: 173 INKQNQDDLLVMELIAIPLSFLVLVWVFGGLLAAALPMALGALAVVGSMSVLRLVTFTTD 232 Q + I+ + FL L ++ M + L +VG + L D Sbjct: 868 SGNQ----APALVAISFVVVFLCLAALYESWSIPVSVMLVVPLGIVGVLLAATLFNQKND 923 Query: 233 VSIFALNLSTALGLALAIDYTLLIISRYRDEL-AEGSSREEALVRTMATSGRTVLFSAVT 291 V F + L T +G L+ +LI+ +D + EG EA + + R +L +++ Sbjct: 924 V-YFMVGLLTTIG--LSAKNAILIVEFAKDLMEKEGKGVVEATLMAVRMRLRPILMTSLA 980 Query: 292 VALSMSATVAFPMYF--------LKSFAYAGVATVAFVATASIVVTPAAIVLL 336 L + P+ + + + +I P V++ Sbjct: 981 FILGV-----LPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIFFVPVFFVVI 1028
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_0990	HTHTETR	67	1e-15	TetR bacterial regulatory protein HTH signature.
>HTHTETR#TetR bacterial regulatory protein HTH signature. Length = 215 Score = 66.6 bits (162), Expect = 1e-15 Identities = 35/164 (21%), Positives = 61/164 (37%), Gaps = 11/164 (6%) Query: 4 ARAPRGSGDLLRHEILDAATELLLQTRQARAVSIRSVAERVGVTSPSIYLHFQDKDALLD 63 AR + R ILD A L Q + + S+ +A+ GVT +IY HF+DK L Sbjct: 2 ARKTKQEAQETRQHILDVALRLFSQ-QGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFS 60 Query: 64 AVCARYLARLDE-EMERAAMGHTCVVEVLRAQGLAYVRFALQTPELYRLATM-------- 114 + + + E E+E A + VLR + + + L + Sbjct: 61 EIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVTEERRRLLMEIIFHKCEFV 120 Query: 115 GEWRSGSNVDSALDSSAFRHMCASVQAMMDEGIYRAD-DPTTIA 157 GE L ++ + +++ ++ + AD A Sbjct: 121 GEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMTRRAA 164
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_1004	PF03544	33	0.001	Gram-negative bacterial tonB protein
>PF03544#Gram-negative bacterial tonB protein Length = 243 Score = 33.4 bits (76), Expect = 0.001 Identities = 29/119 (24%), Positives = 35/119 (29%), Gaps = 1/119 (0%) Query: 155 HAAGPAPVAAPAGAPPASAPAPAAAAPASAPGTAPAPAAAPGPAPAAPAPAP-AAAAPAP 213 PAP + A A A P P P P P P AP P P Sbjct: 40 VIELPAPAQPISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKP 99 Query: 214 APAPAAPPAAPVAAPVAAPAPVPAPAPAAAPAPEAAAPAPAPAPAPAAAPGFGPDAPPT 272 P P P V P PV + + A P + A A + P + P Sbjct: 100 KPKPKPKPVKKVEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPR 158 Score = 31.1 bits (70), Expect = 0.006 Identities = 30/132 (22%), Positives = 38/132 (28%), Gaps = 8/132 (6%) Query: 131 VPHVPAPGAEPGTLAHLPAGIDPAHA--------AGPAPVAAPAGAPPASAPAPAAAAPA 182 V +PAP PA ++P A P P P PP AP Sbjct: 40 VIELPAPAQPISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKP 99 Query: 183 SAPGTAPAPAAAPGPAPAAPAPAPAAAAPAPAPAPAAPPAAPVAAPVAAPAPVPAPAPAA 242 P A+P APA P ++ A + P A P A Sbjct: 100 KPKPKPKPVKKVEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRA 159 Query: 243 APAPEAAAPAPA 254 + PA A Sbjct: 160 LSRNQPQYPARA 171

MUL_1125

MUL_1167

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
MUL_1125	2	10	1.197323	transposase for IS2404
MUL_1126	2	11	-1.320166	hypothetical protein
MUL_1127	2	19	-1.543681	hypothetical protein
MUL_1129	3	29	-3.065960	hypothetical protein
MUL_1130	3	33	-2.802610	hypothetical protein
MUL_1131	5	33	-2.245557	esterase LipC
MUL_1132	4	28	-2.815634	enoyl-CoA hydratase
MUL_1133	4	26	-2.123556	long-chain-fatty-acid-CoA ligase
MUL_1134	3	24	-1.887388	hypothetical protein
MUL_1135	2	22	-2.461933	fatty-acid-CoA ligase
MUL_1136	1	18	-2.266589	transcriptional regulatory protein
MUL_1138	0	16	-2.944978	enoyl-CoA hydratase
MUL_1140	5	13	2.573914	aldehyde dehydrogenase
MUL_1141	5	12	1.297741	methyltransferase
MUL_1142	3	13	0.720017	glycosyltransferase
MUL_1143	4	13	1.464624	transmembrane protein
MUL_1144	4	12	1.526837	hypothetical protein
MUL_1145	2	11	1.422542	integral membrane acyltransferase
MUL_1147	-1	9	-0.835742	transposase for IS2404
MUL_1148	0	12	0.227292	non-IS element not present in Mycobacterium
MUL_1149	-1	13	0.767448	non-IS element not present in Mycobacterium
MUL_1150	1	12	2.677720	non-IS element not present in Mycobacterium
MUL_1151	1	10	2.507633	hypothetical protein
MUL_1152	3	9	3.019594	hypothetical protein
MUL_1153	3	9	2.789572	hypothetical protein
MUL_1154	3	8	3.522487	hypothetical protein
MUL_1155	2	8	3.767498	hypothetical protein
MUL_1156	1	12	1.960954	non-IS element not present in Mycobacterium
MUL_1157	1	10	2.780899	hypothetical protein
MUL_1158	1	9	2.730848	hypothetical protein
MUL_1159	1	10	3.128840	transposase for IS2404
MUL_1160	0	13	1.383489	hypothetical protein
MUL_1161	-1	10	1.858947	hypothetical protein
MUL_1162	-2	12	1.844963	hypothetical protein
MUL_1163	-1	15	1.188185	hypothetical protein
MUL_1164	0	13	1.026350	hypothetical protein
MUL_1165	0	17	0.648380	non-IS element not present in Mycobacterium
MUL_1166	1	20	0.341009	PE-PGRS family protein
MUL_1167	2	19	-0.175926	transposase for IS2606

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_1136	PERTACTIN	32	0.003	Pertactin signature.
>PERTACTIN#Pertactin signature. Length = 922 Score = 31.6 bits (71), Expect = 0.003 Identities = 33/131 (25%), Positives = 41/131 (31%), Gaps = 8/131 (6%) Query: 60 ADLAIVSTKADELRQASKIARDGAGTIGIAQRRVLHAVEDAHNAGFTVGEDFSVTDIRTS 119 +D +V A Q R+ +L A FT+ DI T Sbjct: 491 SDKLVVMRDASG--QHRLWVRNSGSEPASGNTMLLVQTPRGSAATFTLANKDGKVDIGTY 548 Query: 120 RRSAEQAARQAQAQAQAQATDIRQRAVEPVPPPAPGRLPPLTPGEMATPRLPAPPPQPPI 179 R + A+A P P P PG P P + P P PPQPP Sbjct: 549 RYRLAANGNGQWSLVGAKAPP------APKPAPQPGPQPGPQPPQPPQPPQPPQPPQPPQ 602 Query: 180 RGGADLAPAEP 190 R AP P Sbjct: 603 RQPEAPAPQPP 613
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_1145	cloacin	38	1e-04	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 38.2 bits (88), Expect = 1e-04 Identities = 38/109 (34%), Positives = 42/109 (38%), Gaps = 6/109 (5%) Query: 138 GNGGNGGNGGVGQAGGA--GGAAGL-IGNGGSGGIGGPGTTGLAGGAGGSGGWLFGNGGT 194 G G G N G G GG GL +G G S G G GG GSG G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 195 GGRGGIGTTGIGGIGGTGGDAVGL---FGHGGTGGTGGNGPDIFIAGGA 240 G GG G +G G G AV FG G G + I+ GA Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGA 111 Score = 37.4 bits (86), Expect = 2e-04 Identities = 34/91 (37%), Positives = 36/91 (39%), Gaps = 5/91 (5%) Query: 477 GAGGAAGLIGNGGNGGIGGPGTTGMSGGAGGAGGWLIGNGGHGGIGGLGIGIGDSGGAGG 536 G G G GN GGP G+ GGA GW N GG G GI G G G Sbjct: 6 GRGHNTGAHSTSGNIN-GGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGN 64 Query: 537 AGGAAIGLFGNGGTGGTGGYNQAFGAVSGVG 567 GG +GG GTGG A A G Sbjct: 65 GGGNG----NSGGGSGTGGNLSAVAAPVAFG 91 Score = 36.6 bits (84), Expect = 4e-04 Identities = 31/82 (37%), Positives = 38/82 (46%), Gaps = 2/82 (2%) Query: 746 AGGSG-GSTAGVHPAGDGGNSGMSALIGNGGAGGSAGVSPTGVFPSNGGSGGNAQLIGDG 804 +GG G G G H N G + L GGA +G S P GGSG G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENN-PWGGGSGSGIHWGGGS 60 Query: 805 GDGGRGDSGGTGGAGGTGGSLA 826 G G G +G +GG GTGG+L+ Sbjct: 61 GHGNGGGNGNSGGGSGTGGNLS 82 Score = 32.4 bits (73), Expect = 0.008 Identities = 43/135 (31%), Positives = 53/135 (39%), Gaps = 25/135 (18%) Query: 211 TGGDAVGLFGHGGTGGTGGNGPDIFIAGGAGGSGGTGGLLFGNGGAGGGAPWGGADGGAG 270 +GGD G + G T GN I GG G G GG G+G + PWGG G Sbjct: 2 SGGDGRG--HNTGAHSTSGN-----INGGPTGLGVGGGASDGSGWSSENNPWGGGSGSG- 53 Query: 271 GDARLIGNGGGGGYEGSLGVLSGGTAGPGGDGGNAVGLIGNGGAGGAGAAGLSGVTGGIG 330 I GGG G G GG GN+ G G GG A AA ++ + Sbjct: 54 -----IHWGGGSG------------HGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALS 96 Query: 331 GNGGNGAQLFGNGGA 345 G G + + GA Sbjct: 97 TPGAGGLAVSISAGA 111 Score = 32.4 bits (73), Expect = 0.008 Identities = 25/80 (31%), Positives = 32/80 (40%), Gaps = 3/80 (3%) Query: 341 GNGGAGGAGGQSQLASGGTGGSGGMAALFGNGGAGGAAGVSLSGAPGTGGNGGSAQFMGD 400 G G G G + GG G+ GGA +G S P GG+G + G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLG---VGGGASDGSGWSSENNPWGGGSGSGIHWGGG 59 Query: 401 GDSGGLGDSGGSAGAAGAGG 420 G G +G S G +G GG Sbjct: 60 SGHGNGGGNGNSGGGSGTGG 79 Score = 32.4 bits (73), Expect = 0.009 Identities = 25/80 (31%), Positives = 29/80 (36%) Query: 462 GNGGNGGNGLSGQAGGAGGAAGLIGNGGNGGIGGPGTTGMSGGAGGAGGWLIGNGGHGGI 521 G G N G + G +G G + G G GG G+G G GHG Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNG 65 Query: 522 GGLGIGIGDSGGAGGAGGAA 541 GG G G SG G A Sbjct: 66 GGNGNSGGGSGTGGNLSAVA 85 Score = 30.5 bits (68), Expect = 0.035 Identities = 33/98 (33%), Positives = 39/98 (39%), Gaps = 10/98 (10%) Query: 501 MSGGAG---GAGGWLIGNGGHGGIGGLGIGIGDSGGAGGAGGAAIGLFGNGGTGGTGGYN 557 MSGG G G +GG GLG+G G S G+G + N GG G Sbjct: 1 MSGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSE-------NNPWGGGSGSG 53 Query: 558 QAFGAVSGVGGAGGAGGQWYGNGGDGGTSWGGAAAGAG 595 +G SG G GG G G+G G S A G Sbjct: 54 IHWGGGSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFG 91
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_1149	cloacin	40	8e-06	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 40.1 bits (93), Expect = 8e-06 Identities = 30/87 (34%), Positives = 38/87 (43%), Gaps = 7/87 (8%) Query: 169 GNGGAGGDGGIGTTGGGHAGAGGGAALLIGNGGDGGQGGNGINGTGANGGAGGDAGLLLG 228 G G G + G +T G G G G GG +G + N GG +G + Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTG-------LGVGGGASDGSGWSSENNPWGGGSGSGIH 55 Query: 229 TGGNGGSGGAGGNGSNGGNGGNGGNAQ 255 GG G G GGNG++GG G GGN Sbjct: 56 WGGGSGHGNGGGNGNSGGGSGTGGNLS 82 Score = 33.1 bits (75), Expect = 0.001 Identities = 34/90 (37%), Positives = 43/90 (47%), Gaps = 17/90 (18%) Query: 199 NGGDGGQGGNGINGTGANGGAGGDAGLLLGTGGNGGSG-------GAGGNGSNGGNGGNG 251 +GGDG G + T N GG GL +G G + GSG GG+GS GG Sbjct: 2 SGGDGRGHNTGAHSTSGNIN-GGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGS 60 Query: 252 GNAQLIGNGGHGGHAGIGAIRGTAGTGGYG 281 G+ GNGG G++G G +GTGG Sbjct: 61 GH----GNGGGNGNSG-----GGSGTGGNL 81 Score = 31.2 bits (70), Expect = 0.005 Identities = 28/88 (31%), Positives = 39/88 (44%), Gaps = 6/88 (6%) Query: 208 NGINGTGANGGAGGDAGLLLGTGGNGGSGGAGGN-GSNGGNGGNGGNAQLIGNGGHGGHA 266 +G +G G N GA +G + NGG G G G++ G+G + N G G G H Sbjct: 2 SGGDGRGHNTGAHSTSGNI-----NGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHW 56 Query: 267 GIGAIRGTAGTGGYGGLLLGQNGSNGSA 294 G G+ G G G G G G+ + Sbjct: 57 GGGSGHGNGGGNGNSGGGSGTGGNLSAV 84 Score = 30.8 bits (69), Expect = 0.006 Identities = 20/66 (30%), Positives = 23/66 (34%) Query: 158 GGYGGSAGLLIGNGGAGGDGGIGTTGGGHAGAGGGAALLIGNGGDGGQGGNGINGTGANG 217 G G L GGA G + G G G G G GGNG +G G+ Sbjct: 18 GNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNGNSGGGSGT 77 Query: 218 GAGGDA 223 G A Sbjct: 78 GGNLSA 83
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_1152	HTHTETR	63	3e-14	TetR bacterial regulatory protein HTH signature.
>HTHTETR#TetR bacterial regulatory protein HTH signature. Length = 215 Score = 62.7 bits (152), Expect = 3e-14 Identities = 28/157 (17%), Positives = 56/157 (35%), Gaps = 2/157 (1%) Query: 13 RRAAIVEAAEAEFGAHGFSQGSLNVIARRARVAKGSLFQYFADKRDLYAYIADIANQRVR 72 R I++ A F G S SL IA+ A V +G+++ +F DK DL++ I +++ + Sbjct: 12 TRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSEIWELSESNIG 71 Query: 73 THIEGLIREL--DSSRPFFEFLTDLLDGWVAYFAEHPRERALHAAATLEVDTDARISVRS 130 + D E L +L+ V + + + Sbjct: 72 ELELEYQAKFPGDPLSVLREILIHVLESTVTEERRRLLMEIIFHKCEFVGEMAVVQQAQR 131 Query: 131 VIHRHYLEVLRPLVRDALARGDLRADSDTDALLSLLL 167 + + + ++ + L AD T ++ Sbjct: 132 NLCLESYDRIEQTLKHCIEAKMLPADLMTRRAAIIMR 168
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_1155	cloacin	36	5e-04	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 36.2 bits (83), Expect = 5e-04 Identities = 34/90 (37%), Positives = 40/90 (44%), Gaps = 6/90 (6%) Query: 651 AGGDG-GAATGTGGAGGNGVAGGAASGLGVGIGGAGGHGGAA---PTGNGGSGGAGAGGL 706 +GGDG G TG GN G +GLGVG G + G G ++ P G G G GG Sbjct: 2 SGGDGRGHNTGAHSTSGN--INGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGG 59 Query: 707 GLIGAGGNGGGAGAGGAGGNGGDAGAGVAA 736 G GG G +G G G A A A Sbjct: 60 SGHGNGGGNGNSGGGSGTGGNLSAVAAPVA 89 Score = 34.7 bits (79), Expect = 0.001 Identities = 28/86 (32%), Positives = 35/86 (40%) Query: 392 GSGVGGVGGVGGAATGAGATAAAGGAGGLGLAAVGSGTGGAGGLGGTGFGLIAAGGDGGG 451 G G G G + G GG G + GG+G G+ GG G G Sbjct: 4 GDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHG 63 Query: 452 AGTGVGSNGGDGGGGGGAHAVLAAIA 477 G G G++GG G GG AV A +A Sbjct: 64 NGGGNGNSGGGSGTGGNLSAVAAPVA 89 Score = 34.7 bits (79), Expect = 0.001 Identities = 23/82 (28%), Positives = 27/82 (32%) Query: 695 NGGSGGAGAGGLGLIGAGGNGGGAGAGGAGGNGGDAGAGVAAADGGDGGNAGLVINGTYE 754 +GG G G NGG G G GG +G G G +G+ G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 755 ASPYGNGGNGVNGGSGGKGGSA 776 G GN G G SA Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSA 83 Score = 34.3 bits (78), Expect = 0.002 Identities = 35/103 (33%), Positives = 42/103 (40%), Gaps = 7/103 (6%) Query: 144 GNGGNGGSGSAGMAGGAGGSAGLIGNGGAGGAGGADAAGGPGGAG---GWLWGGGGAGGL 200 G G N G+ S GG GL GGA G + P G G G WGGG G Sbjct: 6 GRGHNTGAHSTS-GNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGN 64 Query: 201 GGPASGAGNGGAGGAGGAGGAFIAIGGVGGDGGAASSGIGGVG 243 GG G GN G G G + +A G ++ G GG+ Sbjct: 65 GG---GNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 34.3 bits (78), Expect = 0.002 Identities = 31/93 (33%), Positives = 36/93 (38%), Gaps = 4/93 (4%) Query: 136 GGPGGLLFGNGGNGGSGSAGM----AGGAGGSAGLIGNGGAGGAGGADAAGGPGGAGGWL 191 GGP GL G G + GSG + GG+G G G G GG +GG G GG L Sbjct: 22 GGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNGNSGGGSGTGGNL 81 Query: 192 WGGGGAGGLGGPASGAGNGGAGGAGGAGGAFIA 224 G PA G + GA A Sbjct: 82 SAVAAPVAFGFPALSTPGAGGLAVSISAGALSA 114 Score = 33.9 bits (77), Expect = 0.003 Identities = 32/118 (27%), Positives = 38/118 (32%) Query: 589 TGTAGSGGNGGFVENFDFFGFGVAHGGDGGSGGAASGAGGIGGAGGNGGSGTTPLFGAYS 648 +G G G N G G G GG SG G G G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 649 GGAGGDGGAATGTGGAGGNGVAGGAASGLGVGIGGAGGHGGAAPTGNGGSGGAGAGGL 706 G GG G + G G GGN A A G G GG A + + G+ A + Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAAIADI 119 Score = 33.5 bits (76), Expect = 0.003 Identities = 38/114 (33%), Positives = 45/114 (39%), Gaps = 5/114 (4%) Query: 445 AGGDGGGAGTGVGSNGGDGGGGGGAHAVLAAIAGSGGQGQAGTSGFGGFGGSGGSAESLF 504 +GGDG G TG S G+ GG V G G +S +GG GS Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGV----GGGASDGSGWSSENNPWGGGSGSGIHWG 57 Query: 505 FSIGGAGGAGGDASTGGGGLGGNGGVAVAHSPIGID-IGIGGAGGHGGSGTSGA 557 G G G S GG G GGN A G + GAGG S ++GA Sbjct: 58 GGSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGA 111 Score = 33.5 bits (76), Expect = 0.003 Identities = 31/110 (28%), Positives = 43/110 (39%), Gaps = 1/110 (0%) Query: 324 GQGGAGGIGGAASTGMAGAGGSGGSCVAFDFVGFAAAHGGAGGTGGAATGVGATAGAAGS 383 G+G G + G G G A D G+++ + GG G+ G +G Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNG 65 Query: 384 GGLGVAIVGSGVGGVGGVGGAATGAGATA-AAGGAGGLGLAAVGSGTGGA 432 GG G + GSG GG A G A + GAGGL ++ A Sbjct: 66 GGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAA 115 Score = 33.1 bits (75), Expect = 0.004 Identities = 30/83 (36%), Positives = 35/83 (42%), Gaps = 3/83 (3%) Query: 626 AGGIGGAGGNGGSGTTPLFGAYSGGAGGDGGAATGTGGAGGNGVAGGAASGLGVGIGGAG 685 +GG G G T+ G G GGA+ G+G + N GG G G GI G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGG---GSGSGIHWGG 58 Query: 686 GHGGAAPTGNGGSGGAGAGGLGL 708 G G GNG SGG G L Sbjct: 59 GSGHGNGGGNGNSGGGSGTGGNL 81 Score = 31.6 bits (71), Expect = 0.013 Identities = 30/93 (32%), Positives = 40/93 (43%), Gaps = 2/93 (2%) Query: 669 VAGGAASGLGVGIGGAGGHGGAAPTGNGGSGGAGAGGLGLIGAGGNGGGAGAGGAGGNGG 728 ++GG G G G+ PTG G GGA G GGG+G+G GG Sbjct: 1 MSGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSG--IHWGG 58 Query: 729 DAGAGVAAADGGDGGNAGLVINGTYEASPYGNG 761 +G G +G GG +G N + A+P G Sbjct: 59 GSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFG 91 Score = 31.6 bits (71), Expect = 0.015 Identities = 30/82 (36%), Positives = 34/82 (41%), Gaps = 4/82 (4%) Query: 663 GAGGNGVAGGAASGLGVGIGGAGGHGGAAPTGNGGSGGAGAGGLGLIGAGGNGGGAGAGG 722 G G G GA S G GG G G G G S G+G GG+G G GG Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGV----GGGASDGSGWSSENNPWGGGSGSGIHWGG 58 Query: 723 AGGNGGDAGAGVAAADGGDGGN 744 G+G G G + G GGN Sbjct: 59 GSGHGNGGGNGNSGGGSGTGGN 80 Score = 30.8 bits (69), Expect = 0.024 Identities = 35/120 (29%), Positives = 44/120 (36%), Gaps = 4/120 (3%) Query: 263 GGDATTGIGGAGGAGGMATARIPAGINFNVGGAGGHGGAGATGGAGGGGGSAYSGYVGIA 322 GGD GA G P G+ G + G G + GGG GS G Sbjct: 3 GGDGRGHNTGAHSTSGNINGG-PTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 323 FGQGGAGGIGGAASTGMAGAGGSGGSCVAFDFVGFAAAHGGAGGTGGAATGVGATAGAAG 382 G GG G G S G G + + VAF F + GAGG + + +A A Sbjct: 62 HGNGGGNGNSGGGS-GTGGNLSAVAAPVAFGFPALSTP--GAGGLAVSISAGALSAAIAD 118
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_1163	HTHTETR	79	3e-20	TetR bacterial regulatory protein HTH signature.
>HTHTETR#TetR bacterial regulatory protein HTH signature. Length = 215 Score = 78.9 bits (194), Expect = 3e-20 Identities = 41/215 (19%), Positives = 77/215 (35%), Gaps = 12/215 (5%) Query: 1 MAGGTKRLPRAIREQQMLDAAVQMFSANGYHETSMDTIAAAAQISKPMLYLYYGSKEDLF 60 MA TK+ + R+ +LD A+++FS G TS+ IA AA +++ +Y ++ K DLF Sbjct: 1 MARKTKQEAQETRQH-ILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLF 59 Query: 61 GACLNREMSRFIDAVRADI-DLSQSPKDLLRNAIVSFLRYIDQNQASWIVMYTQAVSSQA 119 S + P +LR ++ L + ++M + Sbjct: 60 SEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVTEERRRLLMEIIFHKCEF 119 Query: 120 F------AQTVREGREQIVELVAGMLRAGTRSPRSDAEIDMMAAA--LVGAGEAVANRLS 171 Q R + + + L+ + A++ AA + G + Sbjct: 120 VGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMTRRAAIIMRGYISGLMENWL 179 Query: 172 AGDTDVD--EAAEMMIDLLWRGLKGAPSDREIGSN 204 D + A + +L P+ R +N Sbjct: 180 FAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATN 214
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_1166	DHBDHDRGNASE	98	2e-25	2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase signature. Length = 261 Score = 98.2 bits (244), Expect = 2e-25 Identities = 69/259 (26%), Positives = 113/259 (43%), Gaps = 20/259 (7%) Query: 211 LEGKVAIVTGAARGIGQTIAEVFARDGASVVAIDVESAADALAETAASVGG---TPLWLD 267 +EGK+A +TGAA+GIG+ +A A GA + A+D ++ D Sbjct: 6 IEGKIAFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHAEAFPAD 65 Query: 268 VTADDAVDKITEHLRDHHGGKADILVNNAGITRDKLLANMDDARWDSVIAVNLLAPLRLT 327 V A+D+IT + G DILVN AG+ R L+ ++ D W++ +VN + Sbjct: 66 VRDSAAIDEITARIEREMGP-IDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGVFNAS 124 Query: 328 EGLVGNGSIGAGGRVIGLSSIAGIAGNRGQTNYGATKAGMIGIAQALAPSLAEKDITINA 387 + G ++ + S Y ++KA + + L LAE +I N Sbjct: 125 RSVSKYMMDRRSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLELAEYNIRCNI 184 Query: 388 VAPGFIETKMTAAI-------------PLATREVGRRLNSLLQGGQPVDVAEAIAYFASP 434 V+PG ET M ++ L T + G L L +P D+A+A+ + S Sbjct: 185 VSPGSTETDMQWSLWADENGAEQVIKGSLETFKTGIPLKKL---AKPSDIADAVLFLVSG 241 Query: 435 ASNAVTGNVIRVCGQAMIG 453 + +T + + V G A +G Sbjct: 242 QAGHITMHNLCVDGGATLG 260

MUL_1255

MUL_1287

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
MUL_1255	0	17	-3.646117	acyl-CoA dehydrogenase
MUL_1256	0	20	-3.648645	membrane protein
MUL_1259	-1	19	-4.467779	transposase for IS2404
MUL_1260	-1	13	-3.525638	transmembrane ABC transporter ATP-binding
MUL_1261	-1	14	-2.996670	hypothetical protein
MUL_1262	0	16	-3.084057	transmembrane ATP-binding protein ABC
MUL_1263	0	14	-2.541997	hypothetical protein
MUL_1264	0	13	-2.772293	AsnC family transcriptional regulator
MUL_1265	0	13	-2.122012	hypothetical protein
MUL_1266	-1	15	-1.854629	ornithine aminotransferase RocD1 and RocD2
MUL_1267	-1	15	-2.155602	cationic amino acid transport integral membrane
MUL_1268	1	17	-0.934307	hypothetical protein
MUL_1269	3	19	-1.321955	periplasmic sugar-binding lipoprotein UspC
MUL_1270	4	19	-0.450996	sugar-transport integral membrane protein ABC
MUL_1271	3	19	-1.561571	sugar-transport integral membrane protein ABC
MUL_1273	2	20	-3.127557	hypothetical protein
MUL_1274	0	15	-2.512590	hypothetical protein
MUL_1275	-2	12	-3.153830	hypothetical protein
MUL_1277	-1	13	-3.145509	hypothetical protein
MUL_1278	0	11	-2.294356	metal cation transporter p-type ATPase a
MUL_1280	0	10	-1.706281	hypothetical protein
MUL_1281	0	11	0.374518	hypothetical protein
MUL_1282	0	11	0.468924	hypothetical protein
MUL_1286	1	13	1.431754	hypothetical protein
MUL_1287	2	12	1.280805	N-term transposase for IS2404

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_1274	PF06776	27	0.046	Invasion associated locus B
>PF06776#Invasion associated locus B Length = 214 Score = 26.8 bits (59), Expect = 0.046 Identities = 8/59 (13%), Positives = 18/59 (30%), Gaps = 5/59 (8%) Query: 7 HVLGALAAIPLLSACATTTHQAGQESTIAPPTKAATAVTATV-----PAQPASPGSSTQ 60 H + AL AI + A + + + + A + + A + + Sbjct: 19 HAVPALKAIQMGPAELSPMLASCRRLARRNGARLMLAGAMAIALSFGWSDRADAQGAVR 77
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_1277	IGASERPTASE	27	0.014	IgA-specific serine endopeptidase (S6) signature.
>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature. Length = 1541 Score = 27.0 bits (59), Expect = 0.014 Identities = 17/51 (33%), Positives = 19/51 (37%), Gaps = 3/51 (5%) Query: 40 VTFDEDRQRPHTGNGAQVLATLRNTAINLHHLNGADNIAEACRITALTANR 90 V E+ TGN L N I HLN ADN + LT N Sbjct: 846 VRLTENSHWHLTGNSDVHQLDLANGHI---HLNSADNSNNVTKYNTLTVNS 893

MUL_1336

MUL_1371

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
MUL_1336	0	13	3.337113	hypothetical protein
MUL_1338	3	16	3.344506	peroxiredoxin AhpE
MUL_1339	2	16	3.761995	*hypothetical protein
MUL_1340	-2	16	1.292984	hypothetical protein
MUL_1341	-2	14	2.168799	hypothetical protein
MUL_1342	0	12	1.168855	cobalamin biosynthesis protein
MUL_1343	1	11	0.604477	transmembrane protein
MUL_1344	2	12	-0.304895	phosphotyrosine protein phosphatase PtpA
MUL_1345	3	13	-0.768997	hypothetical protein
MUL_1347	2	13	-2.322415	hypothetical protein
MUL_1348	1	13	-2.465386	hypothetical protein
MUL_1349	0	18	-3.098026	hypothetical protein
MUL_1351	-1	15	-3.396022	bifunctional RNase H/acid phosphatase
MUL_1352	-2	18	-3.224955	hypothetical protein
MUL_1353	2	20	0.402837	hypothetical protein
MUL_1354	2	17	0.230774	3-methyl-2-oxobutanoate
MUL_1355	1	13	0.912936	exported protease
MUL_1357	2	12	1.038541	cytochrome C oxidase polypeptide I CtaD
MUL_1359	2	12	1.836958	exported protease
MUL_1363	2	13	2.605141	glutamine synthetase
MUL_1364	-1	13	-0.107320	bifunctional glutamine-synthetase
MUL_1365	0	15	0.312131	hypothetical protein
MUL_1366	1	17	-0.827944	PE-PGRS family protein
MUL_1367	1	17	-1.532421	non-IS element not present in Mycobacterium
MUL_1368	1	16	-0.417755	non-IS element not present in Mycobacterium
MUL_1369	0	16	-0.086077	glutamine synthetase GlnA1
MUL_1370	0	14	-0.271172	hypothetical protein
MUL_1371	2	14	-0.578046	transmembrane protein

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_1339	cloacin	38	1e-04	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 38.2 bits (88), Expect = 1e-04 Identities = 36/122 (29%), Positives = 48/122 (39%), Gaps = 3/122 (2%) Query: 361 GAGGAAGNAGLISGTGDVGGQGGVGGFGEGGAGGDGGGAGLIGNGGSGGTGGNAVGNSGV 420 G G N G S +G++ G G G G + G G + GG G+G + G SG Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 421 GGHGGAGGRGGRLYGNGGVGGNGGFSGPITAGGAGGTGGTGGSAGLPGDGGAGGAGGASG 480 G GG G GG G+G G + P+ G + G + GA A A Sbjct: 63 GNGGGNGNSGG---GSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAAIADI 119 Query: 481 FA 482 A Sbjct: 120 MA 121 Score = 35.1 bits (80), Expect = 0.001 Identities = 29/84 (34%), Positives = 35/84 (41%), Gaps = 8/84 (9%) Query: 464 AGLPGDGGAGGAGGASGFAGGGTGGIGGTGGLPFGAA--------GDGGNGGFGAGGRGG 515 +G G G GA SG GG G+G GG G+ G G G GG G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 516 SGGAGGDAWLFGSGGSGGSGGAGA 539 G GG+ G G+GG+ A A Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVA 85 Score = 34.3 bits (78), Expect = 0.002 Identities = 34/107 (31%), Positives = 42/107 (39%), Gaps = 5/107 (4%) Query: 132 GGDGGILFGSGGAGGSGAGGQDGGAGGRAGLFGNGGAGGAGGTGQTQGGAGGAGGLFFGN 191 GGDG G S +G +GG G G G GG G+G + G Sbjct: 3 GGDGR---GHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGG 59 Query: 192 GGAGGPGGSGGLNGGAGGAGGVGGLLFGAGGAGGAGGSGTSGIGGLG 238 G G GG+G GG+G G + + A A G T G GGL Sbjct: 60 SGHGNGGGNGNSGGGSGTGGNLSAV--AAPVAFGFPALSTPGAGGLA 104 Score = 34.3 bits (78), Expect = 0.002 Identities = 35/100 (35%), Positives = 40/100 (40%), Gaps = 7/100 (7%) Query: 164 GNGGAGGAGGTGQTQGGA-GGAGGLFFGNGGAGGPGGS------GGLNGGAGGAGGVGGL 216 G G G G T G GG GL G G + G G S GG +G GG G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 217 LFGAGGAGGAGGSGTSGIGGLGGDGGSAGALSISAGGAGG 256 G G GGSGT G + G ++S GAGG Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG 102 Score = 33.9 bits (77), Expect = 0.002 Identities = 38/123 (30%), Positives = 48/123 (39%), Gaps = 6/123 (4%) Query: 257 NGGTGLSGFGGAGGAGGNA-GLYGHGGGGGAGGTGTGMGVDNDGIGGAGGAGGAGGWLIG 315 +GG G GA GN G G GG G+G +N+ GG G+G G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWG---- 57 Query: 316 TGGTGGTGGFGDGPLGGQGGDGGNAGLFGVGGDGGLGGTGFFGAGGAGGAAGNAGLISGT 375 GG+G G G+G GG G GGN G GAGG + L + Sbjct: 58 -GGSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAAI 116 Query: 376 GDV 378 D+ Sbjct: 117 ADI 119 Score = 32.4 bits (73), Expect = 0.006 Identities = 31/109 (28%), Positives = 37/109 (33%) Query: 144 AGGSGAGGQDGGAGGRAGLFGNGGAGGAGGTGQTQGGAGGAGGLFFGNGGAGGPGGSGGL 203 +GG G G G + G G GG G + G G+G G G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 204 NGGAGGAGGVGGLLFGAGGAGGAGGSGTSGIGGLGGDGGSAGALSISAG 252 +G GG G GG G G L G A+SISAG Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAG 110 Score = 32.4 bits (73), Expect = 0.007 Identities = 23/72 (31%), Positives = 31/72 (43%), Gaps = 4/72 (5%) Query: 120 NGSNGTPGTGAPGGDGGILFGSGGAGGSGAGGQD----GGAGGRAGLFGNGGAGGAGGTG 175 N + GG G+ G G + GSG ++ GG+G G G G GG G Sbjct: 10 NTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNG 69 Query: 176 QTQGGAGGAGGL 187 + GG+G G L Sbjct: 70 NSGGGSGTGGNL 81 Score = 32.4 bits (73), Expect = 0.008 Identities = 33/120 (27%), Positives = 38/120 (31%), Gaps = 11/120 (9%) Query: 397 GGAGLIGNGGSGGTGGNAVGNSGVGGHGGAGGRGGRLYGNGGVGGNGGFSGPITAGGAGG 456 GG G N G+ T GN G G GG G G G SG GG+G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 457 TGGTGGSAGLPGDGGAGGAGGASGFAGGGTGGIGGTGGLPFGAAGDGGNGGFGAGGRGGS 516 G+GG G G GG + F A G GG G+ Sbjct: 63 -----------GNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGA 111 Score = 32.0 bits (72), Expect = 0.010 Identities = 29/90 (32%), Positives = 35/90 (38%) Query: 619 AGGDGGGAGLLLGAGGAGGQGGLGGNTVDGGNGGNGGNAALIGDGGSGGAGGDGGSGDAG 678 +GGDG G + GG G V GG G ++ G G G G +G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 679 DGGAGGDARLVGSGGNGGNGGFSATPAAGG 708 G GG+ G G GGN A P A G Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAAPVAFG 91 Score = 31.2 bits (70), Expect = 0.016 Identities = 33/106 (31%), Positives = 41/106 (38%), Gaps = 5/106 (4%) Query: 322 TGGFGDGPLGGQGGDGGNAGLFGVGGDGGLGGTGFFGAGGAGGAAGNAGLISGTGDVGGQ 381 +GG G G G GN GG GLG G + G+G ++ N G+G Sbjct: 2 SGGDGRGHNTGAHSTSGNIN----GGPTGLGVGGG-ASDGSGWSSENNPWGGGSGSGIHW 56 Query: 382 GGVGGFGEGGAGGDGGGAGLIGNGGSGGTGGNAVGNSGVGGHGGAG 427 GG G G GG G+ GG G S A G + G G Sbjct: 57 GGGSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG 102 Score = 31.2 bits (70), Expect = 0.016 Identities = 28/86 (32%), Positives = 36/86 (41%), Gaps = 2/86 (2%) Query: 330 LGGQGGDGGNAGLFGVGGDGGLGGTGFFGAGGAGGAAGNAGLISGTGDVGGQGGVGGFGE 389 + G G G N G G+ G TG GGA +G + + G G G +G Sbjct: 1 MSGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGI--HWGG 58 Query: 390 GGAGGDGGGAGLIGNGGSGGTGGNAV 415 G G+GGG G G G G +AV Sbjct: 59 GSGHGNGGGNGNSGGGSGTGGNLSAV 84 Score = 30.8 bits (69), Expect = 0.024 Identities = 30/103 (29%), Positives = 33/103 (32%), Gaps = 6/103 (5%) Query: 205 GGAGGAGGVGGLLFGAGGAGGAGGSGTSGIGGLGGDGGSAGALSISAGGAGGNGGTGLSG 264 G GA G + G G GG + G G + G S S GG G G G Sbjct: 8 GHNTGAHSTSGNINGGPTGLGVGGGASDG-SGWSSENNPWGGGSGSGIHWGGGSGHGNGG 66 Query: 265 FGGAGGAGGNAGLYGHGGGGGAGGTGTGMGVDNDGIGGAGGAG 307 G G G G GG A G GAGG Sbjct: 67 GNGNSGGGS-----GTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 30.1 bits (67), Expect = 0.035 Identities = 25/65 (38%), Positives = 28/65 (43%), Gaps = 4/65 (6%) Query: 587 NGGSAGLVGNGGAGGAGGQGGLIVSADDNNGGAGGDGGGAGLLLGAGGAGGQGGLGGNTV 646 NGG GL GGA G S ++ GG G G G G G GG G GG + Sbjct: 21 NGGPTGLGVGGGASDGSGWS----SENNPWGGGSGSGIHWGGGSGHGNGGGNGNSGGGSG 76 Query: 647 DGGNG 651 GGN Sbjct: 77 TGGNL 81 Score = 29.7 bits (66), Expect = 0.044 Identities = 28/76 (36%), Positives = 32/76 (42%), Gaps = 5/76 (6%) Query: 219 GAGGAGGAGGSGTSGIGGLGGDGGSAGALSISAGGAGGNGGTGLSGFGGAGGAGGNAGLY 278 G G GA + + GG G G GA S + N G SG G G G Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGS----- 60 Query: 279 GHGGGGGAGGTGTGMG 294 GHG GGG G +G G G Sbjct: 61 GHGNGGGNGNSGGGSG 76
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_1342	PREPILNPTASE	29	0.014	Type IV prepilin cysteine protease (C20) family sig...
>PREPILNPTASE#Type IV prepilin cysteine protease (C20) family signature. Length = 290 Score = 29.0 bits (65), Expect = 0.014 Identities = 10/46 (21%), Positives = 17/46 (36%), Gaps = 2/46 (4%) Query: 47 LPYMIGAFVLIVGISVAVGVWAGGLTMITMIPFG--LLLGGLVAFI 90 LP ++ L+ + IPFG L + G +A + Sbjct: 231 LPIVLLLSSLVGAFMGIGLILLRNHHQSKPIPFGPYLAIAGWIALL 276
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_1355	NUCEPIMERASE	29	6e-04	Nucleotide sugar epimerase signature.
>NUCEPIMERASE#Nucleotide sugar epimerase signature. Length = 334 Score = 29.4 bits (66), Expect = 6e-04 Identities = 10/26 (38%), Positives = 17/26 (65%) Query: 8 ITGSSGLIGSALAAALRVADHRVLRI 33 +TG++G IG ++ L A H+V+ I Sbjct: 5 VTGAAGFIGFHVSKRLLEAGHQVVGI 30
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_1363	cloacin	36	2e-04	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 35.8 bits (82), Expect = 2e-04 Identities = 34/102 (33%), Positives = 39/102 (38%), Gaps = 3/102 (2%) Query: 196 GRGGIGGQGGTGGEAGGGTGIHAGNGGTGGNGGGGGWLSGDAGAGGQGGASVASNYIAGG 255 GRG G T G GG G G GG G GW S + GG G+ + +G Sbjct: 6 GRGHNTGAHSTSGNINGGPT---GLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 256 GGAGGNGGAAGLFGAGGAGGTGGTGGNGNAPAGGDAGHGGQG 297 G GGNG + G G GG PA G GG Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 34.3 bits (78), Expect = 9e-04 Identities = 31/85 (36%), Positives = 34/85 (40%), Gaps = 1/85 (1%) Query: 233 LSGDAGAGGQGGASVASNYIAGG-GGAGGNGGAAGLFGAGGAGGTGGTGGNGNAPAGGDA 291 +SG G G GA S I GG G G GGA+ G G G GG + Sbjct: 1 MSGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGS 60 Query: 292 GHGGQGGYGGWLAGSGGAGGTGGTA 316 GHG GG G GSG G A Sbjct: 61 GHGNGGGNGNSGGGSGTGGNLSAVA 85 Score = 33.1 bits (75), Expect = 0.002 Identities = 34/89 (38%), Positives = 41/89 (46%), Gaps = 6/89 (6%) Query: 160 GNGGTGGTGGDAVAGLPGVNGGNGGNGPAGGAAGCWGRGGIGGQGGTGGEAGGGTGIHAG 219 G+G TG + +G +NGG G G GGA+ G G G G+GIH G Sbjct: 4 GDGRGHNTGAHSTSG--NINGGPTGLGVGGGASDGSGWSSENNPWG----GGSGSGIHWG 57 Query: 220 NGGTGGNGGGGGWLSGDAGAGGQGGASVA 248 G GNGGG G G +G GG A A Sbjct: 58 GGSGHGNGGGNGNSGGGSGTGGNLSAVAA 86 Score = 32.8 bits (74), Expect = 0.003 Identities = 31/99 (31%), Positives = 36/99 (36%) Query: 27 GNGGNGAAGTNPGVAGGAGGAAGLIGNGGAGGTGGWLFGDGGAGGTGGAGGWLYGNGGAG 86 G+G G + GG GL GGA GW + GG G+G G G G Sbjct: 4 GDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHG 63 Query: 87 GSGGNGGIGELIGNGGAGGAGNTNQVSGYSGNDGNGGNG 125 GGNG G G GG A G+ G G Sbjct: 64 NGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG 102 Score = 32.4 bits (73), Expect = 0.004 Identities = 28/88 (31%), Positives = 39/88 (44%), Gaps = 2/88 (2%) Query: 291 AGHGGQGGYGGWLAGSGGAGGTGGTAGWLYGSGGSGGAGGAGTASFYPGSTGGNGGNGGN 350 +G G+G G + SG G G G G S G+G + + + G +G GG Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLG--VGGGASDGSGWSSENNPWGGGSGSGIHWGGG 59 Query: 351 GGAAQVIGSGGSGGAAGTGGAGGPDSPP 378 G G+G SGG +GTGG + P Sbjct: 60 SGHGNGGGNGNSGGGSGTGGNLSAVAAP 87 Score = 32.0 bits (72), Expect = 0.004 Identities = 31/105 (29%), Positives = 36/105 (34%), Gaps = 5/105 (4%) Query: 52 GNGGAGGTGGWLFGDGGAGGTGGAGGWLYGNGGAGGSGGNGGIGELIGNGGAGGAGNTNQ 111 G G G G G G G G G S G+G E GG G+G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLG-----VGGGASDGSGWSSENNPWGGGSGSGIHWG 57 Query: 112 VSGYSGNDGNGGNGGSGAAGQAGGAGGAAGLIGNGGAGGNGGAGG 156 GN G GN G G+ + AA + A GAGG Sbjct: 58 GGSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG 102 Score = 30.8 bits (69), Expect = 0.011 Identities = 33/107 (30%), Positives = 38/107 (35%), Gaps = 10/107 (9%) Query: 269 GAGGAGGTGGTGGNGNAPAGGDAGHGGQGGYGGWLAGSGGAGGTGGTAGWLYGSGGSGGA 328 G G G T GN N G GG GW + + GG G+ G G G Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNG 65 Query: 329 GGAGTASFYPGSTGGNGGNGGNGGAAQVIGSGGSG-GAAGTGGAGGP 374 GG G GG+G G + V G A T GAGG Sbjct: 66 GGNG---------NSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGL 103

MUL_1443

MUL_1462

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
MUL_1443	0	12	4.357033	hypothetical protein
MUL_1444	-1	10	3.072461	hypothetical protein
MUL_1445	0	9	3.637850	enoyl-CoA hydratase
MUL_1446	0	9	3.568962	aldehyde dehydrogenase
MUL_1447	-1	8	3.080605	hypothetical protein
MUL_1450	0	10	1.323135	hypothetical protein
MUL_1454	-1	11	-0.671266	transmembrane protein
MUL_1455	-1	9	0.355095	transposase for IS2404
MUL_1456	-1	10	0.521496	dehydrogenase
MUL_1458	0	11	0.889552	transmembrane ABC transporter ATP-binding
MUL_1459	1	11	0.568801	bifunctional 5,10-methylene-tetrahydrofolate
MUL_1460	2	16	1.181811	hypothetical protein
MUL_1462	2	13	0.293917	hypothetical protein

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_1444	TCRTETA	29	0.039	Tetracycline resistance protein signature.
>TCRTETA#Tetracycline resistance protein signature. Length = 399 Score = 29.0 bits (65), Expect = 0.039 Identities = 30/138 (21%), Positives = 61/138 (44%), Gaps = 6/138 (4%) Query: 46 LTTMVVIGQIVGALGAGVLANAIGRKKSVVMLLVAYTMFAVLGALSVSLPMLLAARFLL- 104 L ++ + A+ G +A +G ++++++ ++A +L A + M LL Sbjct: 252 LAAFGILHSLAQAMITGPVAARLGERRALMLGMIADGTGYILLAFATRGWMAFPIMVLLA 311 Query: 105 --GLAVGVSIVVVPVYVAESAPAAVRGSLLTVYQLTTVSG-LIVGYLTGYLLAGTHSWRW 161 G+ + ++ V E ++GSL + LT++ G L+ + + + W W Sbjct: 312 SGGIGMPALQAMLSRQVDEERQGQLQGSLAALTSLTSIVGPLLFTAIYAASITTWNGWAW 371 Query: 162 MLGLATVPAMLLLPLLIR 179 + G A +L LP L R Sbjct: 372 IAGAALY--LLCLPALRR 387
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_1445	BLACTAMASEA	30	0.021	Beta-lactamase class A signature.
>BLACTAMASEA#Beta-lactamase class A signature. Length = 286 Score = 29.8 bits (67), Expect = 0.021 Identities = 27/119 (22%), Positives = 45/119 (37%), Gaps = 13/119 (10%) Query: 111 DLDSGAIIAARDPHGRHRPASIIKVLVVM-----VSIKELNQNKAVPGTNDD--SAAEGT 163 DL SG + A R S KV++ V + + + D + + Sbjct: 46 DLASGRTLTAWRADERFPMMSTFKVVLCGAVLARVDAGDEQLERKIHYRQQDLVDYSPVS 105 Query: 164 KVGVNAGGMYTVNQLLHGLLMHSGNDAAHALAIQLGGMQTALEKINMLAAKLGGRDTRV 222 + + A GM TV +L + S N AA+ L +GG + ++G TR+ Sbjct: 106 EKHL-ADGM-TVGELCAAAITMSDNSAANLLLATVGG----PAGLTAFLRQIGDNVTRL 158
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_1450	cloacin	39	4e-05	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 38.5 bits (89), Expect = 4e-05 Identities = 39/108 (36%), Positives = 46/108 (42%), Gaps = 13/108 (12%) Query: 125 GADGTAANPNGGAGGLLYGNGGN---GYSSTTAEVAGGNGGAAGLIGNGGAGGGGGARAA 181 GA T+ N NGG GL G G + G+SS GG+G G G G GGG + Sbjct: 12 GAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNGNS 71 Query: 182 GGYGGHGGWLYGSGGAGGDGGSGAAISAGVVA-GAGGAGGAGGSSSGG 228 GG GSG G A ++ G A GAGG S S G Sbjct: 72 GG---------GSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAG 110 Score = 37.4 bits (86), Expect = 9e-05 Identities = 33/108 (30%), Positives = 43/108 (39%), Gaps = 7/108 (6%) Query: 194 SGGAGGDGGSGAAISAGVVAGAGGAGGAGGSSSGGNANGGAGGAGGAGALGGLLNGAGGN 253 SGG G +GA ++G + G G GG +S G+ G G+ G+ G G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 254 GGAGGAGGNGGDISPLAGGNAGNGGVGGSGGDAGLFGLGGAGGSGGGG 301 G GG GN GG +G GG + FG G GG Sbjct: 62 HGNGGGNGNS-------GGGSGTGGNLSAVAAPVAFGFPALSTPGAGG 102 Score = 37.0 bits (85), Expect = 1e-04 Identities = 23/70 (32%), Positives = 28/70 (40%) Query: 314 GGAGGDGGLLNGTGGAGGAGGDGGSALSGGTGGNGGAGGAGGDAELTGDGGSGGAGGDGG 373 GA G +NG G GG + N GG+G G G G GG+G Sbjct: 11 TGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNGN 70 Query: 374 SPGGTGAAGG 383 S GG+G G Sbjct: 71 SGGGSGTGGN 80 Score = 36.2 bits (83), Expect = 2e-04 Identities = 31/116 (26%), Positives = 38/116 (32%) Query: 255 GAGGAGGNGGDISPLAGGNAGNGGVGGSGGDAGLFGLGGAGGSGGGGGDSVGLQSGAGGG 314 G G G N G S N G G+G GG + G GGG S G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 315 GAGGDGGLLNGTGGAGGAGGDGGSALSGGTGGNGGAGGAGGDAELTGDGGSGGAGG 370 G GG G G G GG + ++ G G G ++ S Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAAIAD 118 Score = 34.3 bits (78), Expect = 8e-04 Identities = 27/83 (32%), Positives = 30/83 (36%), Gaps = 2/83 (2%) Query: 234 AGGAGGAGALGGLLNGAGGNGGAGGAGGNGGDISPLAGGNAGNGGVGGSGGDAGLFGLGG 293 +GG G G NGG G G GG + N GGSG G G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 294 AGGSGGGGGDSVGLQSGAGGGGA 316 G GG G G SG GG + Sbjct: 62 HGNGGGNGNS--GGGSGTGGNLS 82 Score = 33.5 bits (76), Expect = 0.001 Identities = 26/86 (30%), Positives = 31/86 (36%) Query: 316 AGGDGGLLNGTGGAGGAGGDGGSALSGGTGGNGGAGGAGGDAELTGDGGSGGAGGDGGSP 375 +GGDG N + +GG G GG G + G G G GGS Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 376 GGTGAAGGAGGDGGQRGGSPGAPGAA 401 G G G G G GG+ A A Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAAP 87 Score = 32.4 bits (73), Expect = 0.003 Identities = 29/89 (32%), Positives = 37/89 (41%), Gaps = 4/89 (4%) Query: 301 GGDSVGLQSGAGGGGAGGDGGLLNGTGGAGGAGGDGGSALS----GGTGGNGGAGGAGGD 356 GGD G +GA +GG G G + G G S+ + GG+G GG G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 357 AELTGDGGSGGAGGDGGSPGGTGAAGGAG 385 G+G SGG G GG+ A G Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFG 91 Score = 31.6 bits (71), Expect = 0.006 Identities = 34/110 (30%), Positives = 42/110 (38%), Gaps = 4/110 (3%) Query: 158 GGNGGAAGLIGNGGAGGGGGARAAGGYGGHGGWLYGSGGAGGDGGSGAAISAGVVAGAGG 217 G N GA GN G G G G G + S GGSG+ I G +G G Sbjct: 8 GHNTGAHSTSGNINGGPTGLGVGGGASDGSG---WSSENNPWGGGSGSGIHWGGGSGHGN 64 Query: 218 AGGAGGSSSGGNANGGAGGAGGAGALGGLLNGAGGNGGAGGAGGNGGDIS 267 GG G+S GG+ GG A A G + G + G +S Sbjct: 65 -GGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALS 113

MUL_1507

MUL_1528

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
MUL_1507	0	11	-3.260279	transcriptional regulatory protein
MUL_1509	0	14	-4.202747	transcriptional regulatory protein
MUL_1510	-1	14	-4.120898	aconitate hydratase
MUL_1511	-1	15	-4.786431	hypothetical protein
MUL_1512	0	18	-4.586732	cell wall-associated hydrolase
MUL_1518	0	18	-3.930288	invasion protein Inv2
MUL_1519	0	17	-4.430100	transcriptional regulatory protein, MoxR1
MUL_1520	0	16	-3.410055	hypothetical protein
MUL_5113	-1	17	-3.500991	hypothetical protein
MUL_1522	0	15	-3.283055	3-oxoacyl-ACP reductase
MUL_1523	1	16	-3.592184	enoyl-(acyl carrier protein) reductase
MUL_1526	0	13	-3.203311	ferrochelatase
MUL_1527	-1	10	-2.867157	hypothetical protein
MUL_1528	-1	11	-3.298375	transposase for IS2606

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_1512	NUCEPIMERASE	134	6e-39	Nucleotide sugar epimerase signature.
>NUCEPIMERASE#Nucleotide sugar epimerase signature. Length = 334 Score = 134 bits (340), Expect = 6e-39 Identities = 81/353 (22%), Positives = 133/353 (37%), Gaps = 50/353 (14%) Query: 1 MKVLVTGSAGFINGYVVQELLQAGHEVIGIDNYSKYGEVTKSYD-----DHPNYHFVEGD 55 MK LVTG+AGFI +V + LL+AGH+V+GIDN + Y +V+ P + F + D Sbjct: 1 MKYLVTGAAGFIGFHVSKRLLEAGHQVVGIDNLNDYYDVSLKQARLELLAQPGFQFHKID 60 Query: 56 VKDVDLMFELVE--GCEQMVASAARIGGITYFHEYAYDLLAENERIAAAHFDTAIYAYRK 113 + D + M +L E++ S R + Y E + N F + R Sbjct: 61 LADREGMTDLFASGHFERVFISPHR-LAVRYSLENPHAYADSNL----TGFLNILEGCRH 115 Query: 114 GWLKKINVISSSMVFENASIFPTPEKHITECPPPTSTYVFQKLACEYFAHGAYEQYGLPY 173 ++ + SSS V+ P + P S Y K A E AH YGLP Sbjct: 116 NKIQHLLYASSSSVYGLNRKMPFSTDDSVD--HPVSLYAATKKANELMAHTYSHLYGLPA 173 Query: 174 TIIRPFNCVGTGEQRALGGRGIPSGNVKLAMSHVVPDLVQKVVKGQDPLHILGDGSQVRH 233 T +R F G P G +A + + +++G+ + + G R Sbjct: 174 TGLRFFTVYG------------PWGRPDMA----LFKFTKAMLEGK-SIDVYNYGKMKRD 216 Query: 234 YTYGGDLARGIRTCMEHPAALNGD-----------------FNLSTPEATTVLELAEVIW 276 +TY D+A I + + +N+ +++ + + Sbjct: 217 FTYIDDIAEAIIRLQDVIPHADTQWTVETGTPAASIAPYRVYNIGNSSPVELMDYIQALE 276 Query: 277 RKMRPDTPFRYESDPPFEHDVQLRSPDVHKATQVLGFEATTTLDAMLDEVIPW 329 + + P DV S D +V+GF TT+ + + W Sbjct: 277 DAL--GIEAKKNMLPLQPGDVLETSADTKALYEVIGFTPETTVKDGVKNFVNW 327
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_1519	IGASERPTASE	27	0.011	IgA-specific serine endopeptidase (S6) signature.
>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature. Length = 1541 Score = 27.3 bits (60), Expect = 0.011 Identities = 16/51 (31%), Positives = 20/51 (39%), Gaps = 3/51 (5%) Query: 38 VTFDEDRHRAHTGHGAQVLATLRNTAINLHRLNGADNIAEACRITALTANR 88 V E+ H TG+ L N I+ LN ADN + LT N Sbjct: 846 VRLTENSHWHLTGNSDVHQLDLANGHIH---LNSADNSNNVTKYNTLTVNS 893

MUL_1698

MUL_1718

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
MUL_1698	2	13	-1.239255	hypothetical protein
MUL_1699	1	13	-0.210219	C-term transposase for IS2404
MUL_1701	1	12	-0.190576	N-term transposase for IS2404
MUL_1702	1	8	-0.198431	hypothetical protein
MUL_1704	2	11	-0.889511	transposase from IS2404
MUL_1705	2	12	-1.092975	hypothetical protein
MUL_1706	1	16	-1.305707	lipoprotein LprJ
MUL_1707	1	15	-0.854544	hypothetical protein
MUL_1708	0	13	0.343109	phosphatase
MUL_1709	0	12	-1.112340	hypothetical protein
MUL_1710	0	11	-0.638945	cytotoxin/hemolysin, TlyA
MUL_1711	-1	11	0.401039	inorganic polyphosphate/ATP-NAD kinase
MUL_1712	0	15	3.829425	DNA repair protein RecN
MUL_1713	-1	14	3.410169	hypothetical protein
MUL_1714	0	15	3.238858	hypothetical protein
MUL_1715	3	14	5.163281	CTP synthetase
MUL_1716	0	12	3.346214	NUDIX hydrolase
MUL_1718	0	15	3.228682	site-specific tyrosine recombinase XerD

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_1718	cloacin	40	1e-05	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 40.5 bits (94), Expect = 1e-05 Identities = 41/120 (34%), Positives = 49/120 (40%), Gaps = 11/120 (9%) Query: 153 SGGTQSGGTGGSAGLIGNGGNGGNGFLGGAGGAAGSGGWLAGSGGNGGAGGSVTGVGEVG 212 SGG G G+ GN NGG LG GGA+ GW S N GGS +G+ G Sbjct: 2 SGGDGRGHNTGAHSTSGNI-NGGPTGLGVGGGASDGSGW--SSENNPWGGGSGSGIHWGG 58 Query: 213 GAGGAGGSAPLLGWGGNGGAGGDSTQGTGGRGGAGGAGGALAAIGGAGGAGGTGATAGGD 272 G+G G GGNG +GG S G A A+ GAGG + Sbjct: 59 GSGHGNG-------GGNGNSGGGSGTGGNLSAVAAPVAFGFPAL-STPGAGGLAVSISAG 110 Score = 36.2 bits (83), Expect = 3e-04 Identities = 36/106 (33%), Positives = 42/106 (39%), Gaps = 9/106 (8%) Query: 225 GWGGNGGAGGDSTQGTGGRGGAGGAGGALAAIGGAGGAGGTGATAGGDGGVGGEGSGRLF 284 G G N GA S GG G G GGA + G+G ++ + GG GSG + Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGGA---------SDGSGWSSENNPWGGGSGSGIHW 56 Query: 285 GLGGAGGAGGTGTTSGGVGGTGGAGGVAGVLVGAGVGGFGGMGGAG 330 G G G GG SGG GTGG V G G G Sbjct: 57 GGGSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG 102 Score = 34.3 bits (78), Expect = 0.001 Identities = 25/78 (32%), Positives = 34/78 (43%), Gaps = 4/78 (5%) Query: 394 GAGGNGGDGGNGGGLFNIGRGGDGGNGGNAGATGGNGGNIGVVANGTFTQTLFGDGGNGG 453 G G N G G + GG G G GA+ G+G + G + + GG G Sbjct: 6 GRGHNTGAHSTSGNI----NGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 454 NGGNGGTGGTPGTGGSGG 471 +G GG G + G G+GG Sbjct: 62 HGNGGGNGNSGGGSGTGG 79 Score = 33.5 bits (76), Expect = 0.002 Identities = 25/74 (33%), Positives = 30/74 (40%), Gaps = 7/74 (9%) Query: 360 GTATGGDGGAGGQGAALWGAGFGGDGAVGGNSFVGAGGNGGDGGNGGGLFNIGRGGDGGN 419 G GG G G G A G+G+ + G G+G GG G G GG GN Sbjct: 18 GNINGGPTGLGVGGGASDGSGWSSENNPWGG---GSGSGIHWGGGSGH----GNGGGNGN 70 Query: 420 GGNAGATGGNGGNI 433 G TGGN + Sbjct: 71 SGGGSGTGGNLSAV 84 Score = 31.6 bits (71), Expect = 0.008 Identities = 27/104 (25%), Positives = 37/104 (35%), Gaps = 3/104 (2%) Query: 180 GGAGGAAGSGGWLAGSGGNGGAGGSVTGVGEVGGAGGAGGSAPLLGWGGNGGAGGDSTQG 239 GG G +G NGG G G + G+G S+ WGG G+G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTG---LGVGGGASDGSGWSSENNPWGGGSGSGIHWGGG 59 Query: 240 TGGRGGAGGAGGALAAIGGAGGAGGTGATAGGDGGVGGEGSGRL 283 +G G G + G + A G + G+G L Sbjct: 60 SGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGL 103 Score = 31.6 bits (71), Expect = 0.008 Identities = 21/55 (38%), Positives = 23/55 (41%), Gaps = 1/55 (1%) Query: 354 GGVGGFGTATGGDGGAG-GQGAALWGAGFGGDGAVGGNSFVGAGGNGGDGGNGGG 407 GG G G G G+G WG G G GG S G GG G+ G G G Sbjct: 22 GGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNGNSGGGSG 76 Score = 30.8 bits (69), Expect = 0.015 Identities = 28/78 (35%), Positives = 32/78 (41%), Gaps = 1/78 (1%) Query: 324 GGMGGAGTTGGAGDVGGQGVTLTGLGVGGIGGVG-GFGTATGGDGGAGGQGAALWGAGFG 382 GG G TG G TGLGVGG G G+ + GG G G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 383 GDGAVGGNSFVGAGGNGG 400 G+G GNS G+G G Sbjct: 63 GNGGGNGNSGGGSGTGGN 80 Score = 30.1 bits (67), Expect = 0.022 Identities = 25/94 (26%), Positives = 32/94 (34%), Gaps = 5/94 (5%) Query: 382 GGDGAVGGNSFVGAGGNGGDGGNGGGLFNIGRGGDGGNGGNAGATGGNGGNIGVVANGTF 441 GGDG GN G G G+ G G + N GG+G I Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 442 TQTLFGDGGNGGNGGNGGTGGTPGTGGSGGILIG 475 G+GG GN G G G + + + G Sbjct: 63 -----GNGGGNGNSGGGSGTGGNLSAVAAPVAFG 91 Score = 29.7 bits (66), Expect = 0.027 Identities = 28/99 (28%), Positives = 30/99 (30%) Query: 123 GDGANGTATSPNGGAGGFLYGNGGNGYSFTSGGTQSGGTGGSAGLIGNGGNGGNGFLGGA 182 G G N A S +G G G G G + G S G GG G Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNG 65 Query: 183 GGAAGSGGWLAGSGGNGGAGGSVTGVGEVGGAGGAGGSA 221 GG SGG G V GAGG A Sbjct: 66 GGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 29.3 bits (65), Expect = 0.044 Identities = 20/69 (28%), Positives = 24/69 (34%) Query: 324 GGMGGAGTTGGAGDVGGQGVTLTGLGVGGIGGVGGFGTATGGDGGAGGQGAALWGAGFGG 383 GG G G GGA D G G G G+ G + G+GG G G G Sbjct: 22 GGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNGNSGGGSGTGGNL 81 Query: 384 DGAVGGNSF 392 +F Sbjct: 82 SAVAAPVAF 90

MUL_1856

MUL_1879

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
MUL_1856	3	11	2.571083	hypothetical protein
MUL_1857	2	12	1.363113	esterase LipO
MUL_1858	2	12	0.828342	hypothetical protein
MUL_1859	1	11	0.252301	N-term transposase for IS2404
MUL_1860	0	10	-0.223691	C-term transposase for IS2404
MUL_1861	1	11	-1.100814	dehydrogenase
MUL_1862	2	12	-1.768762	hypothetical protein
MUL_1863	2	10	-1.525668	glyceraldehyde 3-phosphate dehydrogenase Gap
MUL_1864	0	11	-0.735810	phosphoglycerate kinase
MUL_1865	-1	10	-0.869017	triosephosphate isomerase
MUL_1866	-2	11	-1.331592	GntR family transcriptional regulator
MUL_1867	-2	12	-0.972362	hypothetical protein
MUL_1869	-2	12	-1.128567	preprotein translocase subunit SecG
MUL_1870	1	12	-3.243024	phosphoenolpyruvate carboxylase
MUL_1873	2	12	-4.476359	PE-PGRS family protein
MUL_1874	1	10	-4.015628	non-IS element not present in Mycobacterium
MUL_1876	2	13	-3.395566	non-IS element not present in Mycobacterium
MUL_1877	2	12	-3.224747	PE-PGRS family protein
MUL_1878	3	13	-3.377626	hypothetical protein
MUL_1879	2	12	-1.765434	6-phosphogluconolactonase

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_1860	PERTACTIN	31	0.019	Pertactin signature.
>PERTACTIN#Pertactin signature. Length = 922 Score = 30.8 bits (69), Expect = 0.019 Identities = 18/64 (28%), Positives = 28/64 (43%) Query: 537 ALTYHRLPWRALPVESPPPAEVTQPAEASQQAEPNQQGESNQPETADPAAKPPATRRPTS 596 +L + P P P P QP + Q +P Q + Q + PA +PPA R ++ Sbjct: 561 SLVGAKAPPAPKPAPQPGPQPGPQPPQPPQPPQPPQPPQPPQRQPEAPAPQPPAGRELSA 620 Query: 597 TTDA 600 +A Sbjct: 621 AANA 624
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_1870	cloacin	40	6e-06	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 40.1 bits (93), Expect = 6e-06 Identities = 29/68 (42%), Positives = 33/68 (48%), Gaps = 3/68 (4%) Query: 121 GTDGTAANPDGGAGGLLIGDG---GKGFSSATAGVAGGSGGSAGLFGSGGAGGTGGGGAL 177 G T+ N +GG GL +G G G G+SS GGSG G G G GG G Sbjct: 12 GAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNGNS 71 Query: 178 GGNGGTGG 185 GG GTGG Sbjct: 72 GGGSGTGG 79 Score = 38.9 bits (90), Expect = 2e-05 Identities = 30/86 (34%), Positives = 36/86 (41%), Gaps = 10/86 (11%) Query: 164 GSGGAGGTGGGGALGGN--GGTGGLLLGNGGAGGMGGTGGSNANGGVGGQAGLIGNGGAG 221 G G G G + GN GG GL +G G + G G + +N GG G G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGS----GIHWGG 58 Query: 222 GDGGGVSGNGGNGGNALLIGNGGDGG 247 G G G G GN G G+G G Sbjct: 59 GSGHGNGGGNGNSGG----GSGTGGN 80 Score = 38.9 bits (90), Expect = 2e-05 Identities = 28/87 (32%), Positives = 37/87 (42%), Gaps = 6/87 (6%) Query: 168 AGGTGGGGALGGNGGTGGLLLGNGGAGGMGGTGGSNANGGVGGQAGLIGNGGAGGDGGGV 227 +GG G G G + +G + NGG G+G GG++ G + G G G G Sbjct: 2 SGGDGRGHNTGAHSTSGNI---NGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGG 58 Query: 228 SGNGGNGGNALLIGNGGDGGTGGGTGT 254 GNGG GN G G GG + Sbjct: 59 GSGHGNGGGN---GNSGGGSGTGGNLS 82 Score = 36.2 bits (83), Expect = 1e-04 Identities = 26/78 (33%), Positives = 33/78 (42%), Gaps = 5/78 (6%) Query: 190 NGGAGGMGGTGGSNANGGVGGQAGLIGNGGAGGDGGGVSGN-----GGNGGNALLIGNGG 244 +GG G TG + +G + G +G GG DG G S GG+G G G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 245 DGGTGGGTGTDGLGGTRG 262 G GG + G GT G Sbjct: 62 HGNGGGNGNSGGGSGTGG 79 Score = 32.4 bits (73), Expect = 0.002 Identities = 26/82 (31%), Positives = 32/82 (39%), Gaps = 1/82 (1%) Query: 139 GDGGKGFSSATAGVAGGSGGSAGLFGSGGAGGTGGGGALGGNGGTGGLLLGNGGAGGMGG 198 G G+G ++ +G G G GG G + G G N GG GG G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGG-GASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 199 TGGSNANGGVGGQAGLIGNGGA 220 G NG GG +G GN A Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSA 83
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_1879	HTHTETR	48	6e-09	TetR bacterial regulatory protein HTH signature.
>HTHTETR#TetR bacterial regulatory protein HTH signature. Length = 215 Score = 48.1 bits (114), Expect = 6e-09 Identities = 25/108 (23%), Positives = 41/108 (37%), Gaps = 1/108 (0%) Query: 23 RWREHRKKVRNEIVEAAFRAIDRLGPE-LSVREIAEEAGTAKPKIYRHFTDKSDLFVAIR 81 + ++ ++ R I++ A R + G S+ EIA+ AG + IY HF DKSDLF I Sbjct: 4 KTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSEIW 63 Query: 82 ERLRDMLWTSIFPSINLATDSAREVIRRSVEEYVSLVDKHPNVLRVFI 129 E + V+R + + + Sbjct: 64 ELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVTEERRRLLME 111

MUL_2020

MUL_2033

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
MUL_2020	0	20	-4.586225	ATP-dependent DNA helicase RecG
MUL_2023	2	23	-5.313891	oxidoreductase
MUL_2024	0	21	-4.364454	hypothetical protein
MUL_2025	-1	16	-3.442538	lipase/esterase LipN
MUL_2026	-1	12	-0.883642	hypothetical protein
MUL_2027	0	10	-0.060141	integral membrane protein
MUL_2028	2	12	1.591573	pyruvate carboxylase
MUL_2029	2	10	2.161732	phosphopantetheine adenylyltransferase
MUL_2030	1	10	2.019144	hypothetical protein
MUL_2031	1	10	2.321320	hypothetical protein
MUL_2032	2	10	2.311651	hypothetical protein
MUL_2033	3	11	2.083546	hypothetical protein

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_2031	DNABINDINGHU	28	0.011	Prokaryotic integration host factor signature.
>DNABINDINGHU#Prokaryotic integration host factor signature. Length = 91 Score = 28.1 bits (63), Expect = 0.011 Identities = 10/29 (34%), Positives = 14/29 (48%) Query: 205 AATLTRRQLGAVLDAAADVMREALAKGGT 233 A LT++ A +DA + LAKG Sbjct: 14 ATELTKKDSAAAVDAVFSAVSSYLAKGEK 42
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_2033	CHANLCOLICIN	35	0.001	Channel forming colicin signature.
>CHANLCOLICIN#Channel forming colicin signature. Length = 522 Score = 35.4 bits (81), Expect = 0.001 Identities = 61/314 (19%), Positives = 111/314 (35%), Gaps = 44/314 (14%) Query: 249 ATMRREHDEAAARLTVAAEELAAHEATLAELTSRAESVQHTWFGLSALAERVGTTVRIAN 308 A +++ E AAR AAE A +A LT R + + + L A R + +A+ Sbjct: 60 AQLKKTQAEQAARAKAAAEAQAKAKANRDALTQRLKDIVNE--ALRHNASRTPSATELAH 117 Query: 309 ERAQHLDVEPVTNSDTDPDALDAEAEQVAIAEQQLLVELAEARDRLDAARAELADREHRA 368 A+ AE E++ +A+ + +AR +AA + E R Sbjct: 118 ANNA---------------AMQAEDERLRLAKAE-----EKARKEAEAAEKAFQEAEQRR 157 Query: 369 AEADRAH------LAAVRAEADRREGLARLAGQVETMRARVESIDDSVARLSERIEHAAA 422 E +R L AE R L+ A VE + ++ + V ++ I+ + Sbjct: 158 KEIEREKAETERQLKLAEAEEKRLAALSEEAKAVEIAQKKLSAAQSEVVKMDGEIKTLNS 217 Query: 423 RA----QQTRAEFETVQARVGELDQGEVGLDEQHERTVAALRLAE------------QRL 466 R AE +T+ + EL Q E E A +R Sbjct: 218 RLSSSIHARDAEMKTLAGKRNELAQASAKYKELDELVKKLSPRANDPLQNRPFFEATRRR 277 Query: 467 AELQVAERDAERQVASLRARIGALSVGLDRKDGAAWLARNHSDAGLFGSVAQLVKVRSGY 526 + ++QV + RI ++ + + A N+ +AG+ ++ Sbjct: 278 VGAGKIREEKQKQVTASETRINRINADITQIQKAISQVSNNRNAGIARVHEAEENLKKAQ 337 Query: 527 EAAVAAVLGSAAEA 540 + + + A +A Sbjct: 338 NNLLNSQIKDAVDA 351

MUL_2208

MUL_2243

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
MUL_2208	2	13	-0.231690	oxidoreductase
MUL_2209	3	13	-0.376509	hypothetical protein
MUL_2210	2	12	-2.336259	dihydrodipicolinate reductase
MUL_2213	0	13	-2.519982	hypothetical protein
MUL_2214	-1	13	-2.676154	hypothetical protein
MUL_2215	-2	16	-2.390205	non-IS element not present in Mycobacterium
MUL_2216	0	17	-2.357624	PPE family protein
MUL_2217	-1	18	-2.784871	transposase for IS2404
MUL_2219	0	17	-2.637832	3-ketoacyl-ACP reductase
MUL_2220	2	19	-2.417359	transposase for IS2404
MUL_2223	1	22	-3.165030	acyl-CoA dehydrogenase FadE18
MUL_2228	-1	17	-2.884234	alanine rich hydrolase
MUL_2230	0	16	-2.509892	thymidylate synthase
MUL_2231	1	14	-2.542536	dihydrofolate reductase DfrA
MUL_2232	1	15	-2.286293	hypothetical protein
MUL_2233	2	15	-1.531803	FAD-dependent thymidylate synthase
MUL_2234	2	14	-1.216771	dihydrodipicolinate synthase
MUL_2236	2	17	-1.684757	C-term transposase for IS2404
MUL_2237	3	18	-2.272661	N-term transposase for IS2404
MUL_2238	3	17	-1.733444	hypothetical protein
MUL_2239	4	18	-1.703961	ferric uptake regulation protein FurA
MUL_2241	5	22	-1.613845	catalase-peroxidase-peroxynitritase T KatG
MUL_2242	3	23	-1.252161	glycine betaine transport integral membrane
MUL_2243	4	19	1.476733	transposase for IS2404

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_2208	INFPOTNTIATR	44	9e-08	Macrophage infectivity potentiator signature.
>INFPOTNTIATR#Macrophage infectivity potentiator signature. Length = 233 Score = 43.8 bits (103), Expect = 9e-08 Identities = 31/101 (30%), Positives = 48/101 (47%), Gaps = 1/101 (0%) Query: 82 QVHTLQAGDGPVVPGTARVSVCYMGVNGRDGSVCDSSYERGAPVVFPLIGVVPGFQKAIA 141 Q + AG G + V+V Y G DG+V DS+ + G P F + V+PG+ +A+ Sbjct: 129 QYKIIDAGTGAKPGKSDTVTVEYTG-TLIDGTVFDSTEKAGKPATFQVSQVIPGWTEALQ 187 Query: 142 GQKVGSTVAVAMTSADGYPDDQPSAGIRPGDALVFAIKVLS 182 GST V + + Y I P + L+F I ++S Sbjct: 188 LMPAGSTWEVFVPADLAYGPRSVGGPIGPNETLIFKIHLIS 228
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_2216	56KDTSANTIGN	27	0.004	Rickettsia 56kDa type-specific antigen protein sign...
>56KDTSANTIGN#Rickettsia 56kDa type-specific antigen protein signature. Length = 533 Score = 26.8 bits (59), Expect = 0.004 Identities = 15/55 (27%), Positives = 23/55 (41%), Gaps = 16/55 (29%) Query: 19 PLGRHEGRSGAAVAWL-----------NPRTPQ-----PTAKSCPRGSPNRLGNP 57 P + R+ A +AWL +P P P + P+G+PN +G P Sbjct: 168 PPLNDQKRAAARIAWLKNCAGIDYMVKDPNNPGHMMVNPVLLNIPQGNPNPVGQP 222
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_2217	IGASERPTASE	30	0.017	IgA-specific serine endopeptidase (S6) signature.
>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature. Length = 1541 Score = 30.0 bits (67), Expect = 0.017 Identities = 17/51 (33%), Positives = 20/51 (39%), Gaps = 3/51 (5%) Query: 283 VTFDEDRHRAHTGNGAQVLATLRNTAINLHRLNGADNIAEACRITALTANR 333 V E+ H TGN L N I+ LN ADN + LT N Sbjct: 846 VRLTENSHWHLTGNSDVHQLDLANGHIH---LNSADNSNNVTKYNTLTVNS 893
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_2228	IGASERPTASE	26	0.014	IgA-specific serine endopeptidase (S6) signature.
>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature. Length = 1541 Score = 26.2 bits (57), Expect = 0.014 Identities = 11/60 (18%), Positives = 20/60 (33%) Query: 18 AKGKAKEVFEAVTGRDDVKREGQAQQDKADAQRDAAKKEAEAEAARRGADVAEERQKANQ 77 + AKE V Q+ + + Q K+ A E + E+ Q+ + Sbjct: 1065 NREVAKEAKSNVKANTQTNEVAQSGSETKETQTTETKETATVEKEEKAKVETEKTQEVPK 1124

MUL_2294

MUL_2313

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
MUL_2294	1	11	3.011104	hypothetical protein
MUL_2295	2	13	3.487877	gluconate kinase
MUL_2296	2	11	1.854401	carboxylesterase, LipT
MUL_2297	1	9	0.929047	lipoprotein LppI
MUL_2298	-1	10	-0.958614	hypothetical protein
MUL_2299	-1	14	-1.875526	hypothetical protein
MUL_2300	0	18	-2.857560	hypothetical protein
MUL_2303	0	20	-3.128763	polyketide synthase
MUL_2304	1	25	-3.227332	hypothetical protein
MUL_2305	2	23	-2.382212	hypothetical protein
MUL_2306	1	15	-0.461912	C-term polyprenol-monophosphomannose synthase
MUL_2307	2	15	-0.506139	N-term polyprenol-monophosphomannose synthase
MUL_2308	3	16	-0.083509	hypothetical protein
MUL_2309	3	16	-0.275773	FxsA protein
MUL_2312	2	12	0.295047	hypothetical protein
MUL_2313	2	10	0.333640	hypothetical protein

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_2294	BLACTAMASEA	297	e-103	Beta-lactamase class A signature.
>BLACTAMASEA#Beta-lactamase class A signature. Length = 286 Score = 297 bits (762), Expect = e-103 Identities = 88/245 (35%), Positives = 123/245 (50%), Gaps = 3/245 (1%) Query: 38 ASTMAVPSPDLESRFAELEQKYEARLGVYVPGTDATAAVEH-RGDERFAFCSTFKGLLGA 96 SP + E + R+G+ + + R DERF STFK +L Sbjct: 15 LPLAVHASPQPLEQIKLSESQLSGRVGMIEMDLASGRTLTAWRADERFPMMSTFKVVLCG 74 Query: 97 AVLHRYSIA--HLGTVITYNSADIRSTSPIIEQHLATGMSIGGLCDATIRYSDGTAANLL 154 AVL R L I Y D+ SP+ E+HLA GM++G LC A I SD +AANLL Sbjct: 75 AVLARVDAGDEQLERKIHYRQQDLVDYSPVSEKHLADGMTVGELCAAAITMSDNSAANLL 134 Query: 155 LQDIGGIAAFNEYLRSLGDSVSRLDQMEPELNRNPPGDVRDTTTPHAIAMDYQQVVLGDA 214 L +GG A +LR +GD+V+RLD+ E ELN PGD RDTTTP ++A ++++ Sbjct: 135 LATVGGPAGLTAFLRQIGDNVTRLDRWETELNEALPGDARDTTTPASMAATLRKLLTSQR 194 Query: 215 LLPEKRDKLIDWLGRSTTGAKRIRAGFPADWRVIDKTGSGEYGRANDVAVVWSPGGTPYV 274 L + +L+ W+ IR+ PA W + DKTG+GE G VA++ + Sbjct: 195 LSARSQRQLLQWMVDDRVAGPLIRSVLPAGWFIADKTGAGERGARGIVALLGPNNKAERI 254 Query: 275 VAIMT 279 V I Sbjct: 255 VVIYL 259
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_2299	DHBDHDRGNASE	43	3e-07	2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase signature. Length = 261 Score = 43.1 bits (101), Expect = 3e-07 Identities = 42/178 (23%), Positives = 59/178 (33%) Query: 9 VVIFGGRSEIGSELARRLAPGATVILAARGADQLDVQVAALKTAGAAAVHTREFDADDVA 68 I G IG +AR LA I A + +V + A A D D A Sbjct: 11 AFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHAEAFPADVRDSA 70 Query: 69 GHGPLVASIVADHGPIGTAVLAFGILGDQARAERDAEHAIAIVHTDYVAQISLLTHLAAA 128 + A I + GPI V G+L E A + + ++ Sbjct: 71 AIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGVFNASRSVSKY 130 Query: 129 MRTAGRGALVVFSSVAGARVRRANYVYGSAKAGLDGFASGLADALHGTGVRLLILRPG 186 M G++V S R + Y S+KA F L L +R I+ PG Sbjct: 131 MMDRRSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLELAEYNIRCNIVSPG 188
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_2313	CHANLCOLICIN	32	0.016	Channel forming colicin signature.
>CHANLCOLICIN#Channel forming colicin signature. Length = 522 Score = 32.0 bits (72), Expect = 0.016 Identities = 54/237 (22%), Positives = 96/237 (40%), Gaps = 25/237 (10%) Query: 220 EFMLDEPDSLARLPEALKQIDPLVEARELLAVAQKK-RKILGDIEQIQQRYASESSDLGI 278 + E LA L E K ++ A++ L+ AQ + K+ G+I+ + R +S Sbjct: 172 KLAEAEEKRLAALSEEAKAVE---IAQKKLSAAQSEVVKMDGEIKTLNSRLSS------S 222 Query: 279 IDLVDAPMVRAYTDHARLAQCPAQIATLDGTVDQLENEYEDVTRSLNLAKAEADSLNAQI 338 I DA M LAQ A+ LD V +L D ++ +A + A Sbjct: 223 IHARDAEMKTLAGKRNELAQASAKYKELDELVKKLSPRANDPLQNRPFFEATRRRVGAGK 282 Query: 339 SGSSANIAPLQSQVTAAETQAEQVERRRTTYEDMLAAQGIEIPDTADEFWNLREEL-LAQ 397 Q QVTA+ET+ ++ T + ++ E L AQ Sbjct: 283 IREEK-----QKQVTASETRINRINADITQIQKAISQVSNNRNAGIARVHEAEENLKKAQ 337 Query: 398 ATELLAKVERNREASTD------AEYAQKATRIARD--DVAKELKRVEHVGSALPEF 446 L ++++ +A+ +Y +K +++A++ D +K K++ +V AL F Sbjct: 338 NNLLNSQIKDAVDATVSFYQTLTEKYGEKYSKMAQELADKSKG-KKIGNVNEALAAF 393

MUL_2325

MUL_2344

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
MUL_2325	2	13	-0.435694	hypothetical protein
MUL_2326	2	10	-0.380748	hypothetical protein
MUL_2327	3	11	-0.379652	cobalamin biosynthesis protein
MUL_2328	2	10	-0.849196	precorrin-8X methylmutase
MUL_2329	1	11	-1.813027	bifunctional protein, CobI-CobJ fusion protein
MUL_2330	0	11	-1.844140	hypothetical protein
MUL_2331	-1	12	-1.639479	membrane transporter
MUL_2332	2	11	-2.098313	class a beta-lactamase, BlaC
MUL_2333	1	11	-1.509978	RNA polymerase sigma factor SigC
MUL_2334	2	12	-1.738356	cobalt-precorrin-6x reductase
MUL_2335	2	11	-0.940285	precorrin-4 C11-methyltransferase, CobM
MUL_2336	2	12	-0.782767	precorrin-6y methyltransferase, CobL
MUL_2337	1	12	-0.429649	short chain dehydrogenase
MUL_2338	-2	16	-0.105363	hypothetical protein
MUL_2339	-3	14	-1.417590	transposase for IS2404
MUL_2340	-2	14	-1.910804	hypothetical protein
MUL_2341	-1	17	-2.852466	hypothetical protein
MUL_2342	-1	19	-2.942928	transposase
MUL_2344	-1	20	-3.455582	hypothetical protein

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_2326	TATBPROTEIN	30	5e-04	Bacterial sec-independent translocation TatB protein...
>TATBPROTEIN#Bacterial sec-independent translocation TatB protein signature. Length = 171 Score = 30.4 bits (68), Expect = 5e-04 Identities = 16/92 (17%), Positives = 35/92 (38%), Gaps = 10/92 (10%) Query: 7 WHWAILAVVVIVLFGAKKLPDAARSLGKSMRIFKSEMREMQSETKAEPSAIE-------- 58 ++ ++ +V+ G ++LP A +++ +R +S +Q+E E E Sbjct: 7 SELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLATTVQNELTQELKLQEFQDSLKKV 66 Query: 59 --TNTANPTPVQSQRIDPAAATGQDQTEARPA 88 + N TP +D + + A Sbjct: 67 EKASLTNLTPELKASMDELRQAAESMKRSYVA 98

MUL_2365

MUL_2383

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
MUL_2365	3	13	-0.213288	sec-independent protein translocase
MUL_2366	3	17	-0.346108	twin arginine translocase protein A
MUL_2367	3	17	0.292914	hypothetical protein
MUL_2368	2	14	0.612758	hypothetical protein
MUL_2369	0	16	0.830427	hypothetical protein
MUL_2370	1	14	0.181982	proteasome PrcA
MUL_2371	0	17	-0.412672	proteasome PrcB
MUL_2372	2	16	-1.119680	hypothetical protein
MUL_2373	2	15	-1.630728	hypothetical protein
MUL_2376	2	17	-3.106690	hypothetical protein
MUL_2377	2	19	-4.676740	integral membrane protein
MUL_2379	1	20	-3.940573	hypothetical protein
MUL_2381	1	20	-3.300645	ATPase
MUL_2383	1	17	-3.203795	lipoprotein LppK

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_2381	2FE2SRDCTASE	25	0.045	Ferric iron reductase signature.
>2FE2SRDCTASE#Ferric iron reductase signature. Length = 262 Score = 24.6 bits (53), Expect = 0.045 Identities = 9/36 (25%), Positives = 13/36 (36%) Query: 33 PAGWRVVFGEAARAACLDYVEQNWPDIRPKSLRERL 68 P + F E R AC + P S + R+ Sbjct: 122 PEHFHAEFHETGRVACFWVDVCEDKNATPHSPQHRM 157

MUL_2480

MUL_5120

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
MUL_2480	3	10	4.010279	NADPH quinone oxidoreductase FadB4
MUL_2481	4	11	4.016828	PPE family protein
MUL_2482	3	12	3.636872	transposase for IS2606
MUL_2483	3	11	3.543678	transposase for IS2404
MUL_2484	3	12	4.242224	oxidoreductase
MUL_2485	4	13	4.545007	hypothetical protein
MUL_2486	1	11	0.689239	hypothetical protein
MUL_2487	1	10	0.000927	transcriptional regulatory protein
MUL_2488	2	13	-0.893277	hypothetical protein
MUL_2489	2	13	-1.134582	hypothetical protein
MUL_5120	0	14	-3.116847	hypothetical protein

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_2481	DHBDHDRGNASE	44	2e-07	2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase signature. Length = 261 Score = 44.3 bits (104), Expect = 2e-07 Identities = 38/173 (21%), Positives = 61/173 (35%), Gaps = 34/173 (19%) Query: 21 VAQIEAAGGR-AVAVRADLTDRDDVAALVTAARDSLGPITILVNNAAFTAPGRPPVPGAA 79 V A R A A AD+ D + + +GPI ILVN A PG Sbjct: 48 VVSSLKAEARHAEAFPADVRDSAAIDEITARIEREMGPIDILVNVAGVLRPG-------- 99 Query: 80 PRAKSSRAAVGKPGWPGFVSVPLAAYRRHFETSVFAAYELMQLSCPDMIAAGAGSIINIT 139 S+ + F + + + M+ +GSI+ + Sbjct: 100 ----------------LIHSLSDEEWEATFSVNSTGVFNASRSVSKYMMDRRSGSIVTVG 143 Query: 140 SVASRLPGDGPYPDRSGGVLPGYGGSKAALEHLTQCAAFDLADHHIAVNALAP 192 S + +P + Y SKAA T+C +LA+++I N ++P Sbjct: 144 SNPAGVPRTS---------MAAYASSKAAAVMFTKCLGLELAEYNIRCNIVSP 187
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_2482	HTHTETR	77	1e-19	TetR bacterial regulatory protein HTH signature.
>HTHTETR#TetR bacterial regulatory protein HTH signature. Length = 215 Score = 77.0 bits (189), Expect = 1e-19 Identities = 27/169 (15%), Positives = 55/169 (32%), Gaps = 11/169 (6%) Query: 12 PGAGRPRDPRIDFAILSATMELLVQIGYSNLSLAAVAERAGTTKSALYRRWSSKAELVHE 71 + IL + L Q G S+ SL +A+ AG T+ A+Y + K++L E Sbjct: 2 ARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSE 61 Query: 72 AAFPVTPSALSAPAGDFAGDIRMMVAATRDV----FTTPVVRAALPGLV------ADMTA 121 + A ++ R++ + V L+ + Sbjct: 62 IWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVTEERRRLLMEIIFHKCEFVG 121 Query: 122 EADLNARVLSRF-TELFATVRIRLQEAVDRGEAHPDVDPNRMIELIGGA 169 E + + E + + L+ ++ D+ R ++ G Sbjct: 122 EMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMTRRAAIIMRGY 170
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_2485	cloacin	37	2e-04	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 37.4 bits (86), Expect = 2e-04 Identities = 32/109 (29%), Positives = 40/109 (36%), Gaps = 4/109 (3%) Query: 232 AGGYGPGGVGGTGGAGGAGGLLAGLVGAGGGHGGTGGFGAGGTGGDGGAGGNAGLFGGPG 291 +GG G G G G +G GGG G+ + GG+G GG G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 292 GAGGTGGVGTGGDGGNGGAGGNAGALFGTG----GAGGAGGSGVAGAGG 336 G G +GG G GG A G GAGG V+ + G Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAG 110 Score = 37.0 bits (85), Expect = 3e-04 Identities = 38/139 (27%), Positives = 46/139 (33%), Gaps = 12/139 (8%) Query: 266 TGGFGAGGTGGDGGAGGNAGLFGGPGGAGGTGGVGTG----------GDGGNGGAGGNAG 315 +GG G G G GN GGP G G GG G G G G G Sbjct: 2 SGGDGRGHNTGAHSTSGNIN--GGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGG 59 Query: 316 ALFGTGGAGGAGGSGVAGAGGVGGAGGNAGLLFSAGGVGGAGGYGSSDGGAGGAGGNGGL 375 + G GG G G G G + F A GAGG S + + Sbjct: 60 SGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAAIADI 119 Query: 376 LYSNGGVGGTGGYGAAAAG 394 + + G G +G A G Sbjct: 120 MAALKGPFKFGLWGVALYG 138 Score = 35.5 bits (81), Expect = 9e-04 Identities = 30/128 (23%), Positives = 43/128 (33%), Gaps = 11/128 (8%) Query: 365 GAGGAGGNGGLLYSNGGVGGTGGYGAAAAGGVGGAGGRAGLAIGGGGAGGAGGEGATTGG 424 G G G N G ++G + G G G+G + GGG+G G +G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 425 DGGAGGTGVLIGNGGNAGVGGTGPAAGATGVGGTSGLLLGLDGFNAPASTSPLHTFQQQA 484 GNGG G G G G + + G + P + + A Sbjct: 63 -----------GNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGA 111 Query: 485 LGAVNAPV 492 L A A + Sbjct: 112 LSAAIADI 119 Score = 33.9 bits (77), Expect = 0.002 Identities = 37/115 (32%), Positives = 44/115 (38%), Gaps = 2/115 (1%) Query: 571 GAGGAGGYSSTADGGVGGAGGAGGLWGGGGIGGTGGFGALNGAAGGVGGAGGLLGGLVGA 630 G G G + GG GL GGG G+ + N GG G+G GG G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 631 GGGDGGAGGYGLTGAGGAGGAGGNSGLLSGPGGSGGTGGAGAVADGAVGGAGGSA 685 G G G G +G GG A P S T GAG +A GA +A Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALS--TPGAGGLAVSISAGALSAA 115 Score = 33.9 bits (77), Expect = 0.003 Identities = 40/134 (29%), Positives = 52/134 (38%), Gaps = 4/134 (2%) Query: 140 GDGGIGGSGTPGTVANPTGGVGGVGGAAGLLGSGGAGGAGGSSAFGDGGAGGVGGWLSGN 199 GDG +G T N GG G+G G S G+G + ++ +G G G+ G Sbjct: 4 GDGRGHNTGAHSTSGNINGGPTGLGVGGG--ASDGSGWSSENNPWGGGSGSGIH--WGGG 59 Query: 200 AGAGGAGGPGLFGFNGGAGGAGGLLGAGGLGGAGGYGPGGVGGTGGAGGAGGLLAGLVGA 259 +G G GG G G G GG + A G G GG + AG L A + Sbjct: 60 SGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAAIADI 119 Query: 260 GGGHGGTGGFGAGG 273 G FG G Sbjct: 120 MAALKGPFKFGLWG 133 Score = 33.5 bits (76), Expect = 0.003 Identities = 35/119 (29%), Positives = 42/119 (35%), Gaps = 12/119 (10%) Query: 336 GVGGAGGNAGLLFSAGGVGGAGGYGSSDGGAGGAGGNGGLLYSNGGVGGTGGYGAAAAGG 395 G G G N G ++G + G G GGA G N GG G G GG Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGL---GVGGGASDGSGWSSENNPWGGGSGSGIHWGGG 59 Query: 396 VGGAGGRAGLAIGGGGAGGAGGEGATTGGDGGAGGTGVLIGNGGNAGVGGTGPAAGATG 454 G G GG G G G+ TGG+ A V G + G G A + Sbjct: 60 SGH---------GNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISA 109 Score = 33.1 bits (75), Expect = 0.004 Identities = 36/118 (30%), Positives = 45/118 (38%), Gaps = 5/118 (4%) Query: 503 NGTPGAAGSGAAGTAGGWVFGDGGADGSGAMSTGADGGAGGAAGMWGTGGSGGAGPAGIG 562 N + G G G G +DGSG S G G +G+ GGSG Sbjct: 10 NTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHG-----N 64 Query: 563 GGLTGGAGGAGGAGGYSSTADGGVGGAGGAGGLWGGGGIGGTGGFGALNGAAGGVGGA 620 GG G +GG G GG S V A G GG+ + GAL+ A + A Sbjct: 65 GGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAAIADIMAA 122 Score = 32.8 bits (74), Expect = 0.006 Identities = 29/80 (36%), Positives = 35/80 (43%), Gaps = 5/80 (6%) Query: 540 GAGGAAGMWGTGGSGGAGPAGIGGGLTGGAGGAGGAGGYSSTADGGVGGAGGAGGLWGGG 599 G G G T G+ GP G+G G GGA G+SS + GG+G GG Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVG-----GGASDGSGWSSENNPWGGGSGSGIHWGGGS 60 Query: 600 GIGGTGGFGALNGAAGGVGG 619 G G GG G G +G G Sbjct: 61 GHGNGGGNGNSGGGSGTGGN 80 Score = 32.4 bits (73), Expect = 0.008 Identities = 26/78 (33%), Positives = 37/78 (47%), Gaps = 2/78 (2%) Query: 637 AGGYGLTGAGGAGGAGGN--SGLLSGPGGSGGTGGAGAVADGAVGGAGGSAGLLFGSGRI 694 +GG G GA GN G G G + G+G ++ G G +G+ +G G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 695 GGDGGFGSNTGGAGGSGG 712 G+GG N+GG G+GG Sbjct: 62 HGNGGGNGNSGGGSGTGG 79 Score = 31.6 bits (71), Expect = 0.012 Identities = 33/105 (31%), Positives = 38/105 (36%), Gaps = 6/105 (5%) Query: 171 GSGGAGGAGGSSAFGDGGAGGVGGWLSGNAGAGGAGGPGLFGFNGGAGGAGGLLGAGGLG 230 G G GA +S +GG G+G G G + G G N GG G G G Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGV------GGGASDGSGWSSENNPWGGGSGSGIHWGGG 59 Query: 231 GAGGYGPGGVGGTGGAGGAGGLLAGLVGAGGGHGGTGGFGAGGTG 275 G G G GG+G G L A G GAGG Sbjct: 60 SGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 30.8 bits (69), Expect = 0.020 Identities = 28/94 (29%), Positives = 37/94 (39%), Gaps = 5/94 (5%) Query: 597 GGGGIGGTGGFGALNGAAGGVGGAGGLLGGLVGAGGGDGGAGGYGLTGAGGAGGAGGNSG 656 GG G G G + +G G G VG G DG GG G+G + G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLG-----VGGGASDGSGWSSENNPWGGGSGSGIHWG 57 Query: 657 LLSGPGGSGGTGGAGAVADGAVGGAGGSAGLLFG 690 SG G GG G +G + + +A + FG Sbjct: 58 GGSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFG 91 Score = 30.8 bits (69), Expect = 0.021 Identities = 41/113 (36%), Positives = 46/113 (40%), Gaps = 7/113 (6%) Query: 120 NGANGAPGTGANGGDGGWLLGDGGIGGSGTPGTVANPTGGVGGVGGAAGLLGSGGAGGAG 179 N + NGG G +G G GSG + NP GG G G G G G G G Sbjct: 10 NTGAHSTSGNINGGPTGLGVGGGASDGSGW-SSENNPWGGGSGSGIHWG--GGSGHGNGG 66 Query: 180 GSSAFGDGGAGGVGGWLSGNAGAGGAGGPGLFGFNGGAGGAGGLLGAGGLGGA 232 G+ GG G GG LS A G P L GAGG + AG L A Sbjct: 67 GNG--NSGGGSGTGGNLSAVAAPVAFGFPAL--STPGAGGLAVSISAGALSAA 115 Score = 30.5 bits (68), Expect = 0.026 Identities = 29/107 (27%), Positives = 39/107 (36%), Gaps = 7/107 (6%) Query: 614 AGGVGGAGGLLGGLVGAGGGDGGAGGYGLTGAGGAGGAGGNSGLLSGPGGSGGTGGAGAV 673 G +G + GG G G G G + G G + G G SG+ G G G GG Sbjct: 11 TGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGN-- 68 Query: 674 ADGAVGGAGGSAGLLFGSGRIGGDGGFGSNTGGAGGSGGLLLGQDGS 720 G +GG +G + FG G+GGL + Sbjct: 69 -----GNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAG 110 Score = 30.1 bits (67), Expect = 0.034 Identities = 33/104 (31%), Positives = 36/104 (34%), Gaps = 4/104 (3%) Query: 159 GVGGVGGAAGLLGSGGAGGAGGSSAFGDGGAGGVGGWLSGNAGAGGAGGPGLFGFNGGAG 218 G G G G + G G + GGA GW S N GG G G+ G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 219 GAGGLLGAGGLGGAGGYGPGGVGGTGGAGGAGGLLAGLVGAGGG 262 G GG G GG G GG A A G A GG Sbjct: 63 GNGG----GNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG 102 Score = 30.1 bits (67), Expect = 0.035 Identities = 36/121 (29%), Positives = 43/121 (35%), Gaps = 14/121 (11%) Query: 300 GTGGDGGNGGAGGNAGALFGTGGAGGAGGSGVAGAGGVGGAGGNAGLLFSAGGV--GGAG 357 G G G N GA +G + G G GG G + G +S+ GG Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGG------------GASDGSGWSSENNPWGGGS 50 Query: 358 GYGSSDGGAGGAGGNGGLLYSNGGVGGTGGYGAAAAGGVGGAGGRAGLAIGGGGAGGAGG 417 G G GG G G GG S GG G G A AA G + GG + G Sbjct: 51 GSGIHWGGGSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAG 110 Query: 418 E 418 Sbjct: 111 A 111

MUL_2544

MUL_2550

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
MUL_2544	3	12	-0.467114	hypothetical protein
MUL_2545	3	14	-0.465653	ABC transporter ATP-binding protein
MUL_2546	3	14	-0.407256	transcriptional regulatory protein Whib-like
MUL_2547	4	17	-0.553702	ATP-dependent DNA helicase II UvrD2
MUL_2549	5	19	-1.397575	glutaredoxin protein
MUL_2550	3	16	-0.530323	transposase for IS2404

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_2545	adhesinb	29	0.010	Adhesin B signature.
>adhesinb#Adhesin B signature. Length = 310 Score = 29.4 bits (66), Expect = 0.010 Identities = 18/50 (36%), Positives = 22/50 (44%), Gaps = 8/50 (16%) Query: 130 VEALEALPDTEIKEALQALPEEFRMAV-------YYADVEGFPYKEIAEI 172 VE L AL D E KE +P E +M V Y++ P I EI Sbjct: 178 VEKLSAL-DKEAKEKFNNIPGEKKMIVTSEGCFKYFSKAYNVPSAYIWEI 226
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_2546	DHBDHDRGNASE	70	2e-16	2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase signature. Length = 261 Score = 70.5 bits (172), Expect = 2e-16 Identities = 50/191 (26%), Positives = 88/191 (46%), Gaps = 10/191 (5%) Query: 3 LNGKTMFISGASRGIGLAIAKRAAQDGANIALIAKTAEPHPKLPGTVYTAAKELEEAGGQ 62 + GK FI+GA++GIG A+A+ A GA+IA + E K+ ++ A+ E Sbjct: 6 IEGKIAFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHAE----- 60 Query: 63 ALPIVGDVRDPDSVSAAVAKTVEQFGGIDICVNNASAINLGSITEVPMKRFDLMNGIQVR 122 A P DVRD ++ A+ + G IDI VN A + G I + + ++ + Sbjct: 61 AFPA--DVRDSAAIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATFSVNST 118 Query: 123 GTYAVSQACIPHLKGRENPHILTL-SPPVQLDKKWLKPTAYMMAKFGMTLCALGIAEEMR 181 G + S++ ++ R + I+T+ S P + + AY +K + + E+ Sbjct: 119 GVFNASRSVSKYMMDRRSGSIVTVGSNPAGVPR--TSMAAYASSKAAAVMFTKCLGLELA 176 Query: 182 DEGIASNTLWP 192 + I N + P Sbjct: 177 EYNIRCNIVSP 187
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_2550	PF03544	32	0.004	Gram-negative bacterial tonB protein
>PF03544#Gram-negative bacterial tonB protein Length = 243 Score = 32.3 bits (73), Expect = 0.004 Identities = 18/97 (18%), Positives = 24/97 (24%), Gaps = 4/97 (4%) Query: 287 LPPVQPTKPPKANEVKIDPPAQAKP---PEQIVVPPGPDPVPAPADDWPVDEALPNP-TD 342 L P Q +PP V+ +P + P E VV P P P P P Sbjct: 60 LEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKPVKKVEQPKRDVK 119 Query: 343 MPVVPFAGSPQLPGNTLADSFAGRGGGTGLSAGAPKL 379 A + S + Sbjct: 120 PVESRPASPFENTAPARPTSSTATAATSKPVTSVASG 156 Score = 29.9 bits (67), Expect = 0.020 Identities = 20/109 (18%), Positives = 31/109 (28%), Gaps = 3/109 (2%) Query: 289 PVQPTKPPKANEVKIDPPAQAKPPEQIVVPPGPDPVPAP---ADDWPVDEALPNPTDMPV 345 P QP ++PP +PP + VV P P+P P P + V E Sbjct: 46 PAQPISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKP 105 Query: 346 VPFAGSPQLPGNTLADSFAGRGGGTGLSAGAPKLKPASFGGAGAASMRP 394 P Q + + P A+ + + Sbjct: 106 KPVKKVEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVA 154

MUL_2627

MUL_2637

Genomic Island

LocusTag	DNBias	CDNBias	%GCBias	Product
MUL_2627	2	15	0.819277	two component sensory transduction
MUL_2628	2	14	0.758301	thymidylate kinase
MUL_2629	2	15	0.326507	transposase for IS2404
MUL_2630	2	17	1.072813	transposase for IS2404
MUL_2631	1	17	1.683764	S-adenosyl-L-homocysteine hydrolase
MUL_2632	2	23	1.644205	TetR family transcriptional regulator
MUL_2633	1	9	1.683094	rubredoxin RubB
MUL_2634	1	9	1.647688	rubredoxin RubA
MUL_2635	2	9	1.482446	cationic amino acid transport integral membrane
MUL_2636	2	8	1.367839	hypothetical protein
MUL_2637	2	8	1.231184	hypothetical protein

MUL_2682

MUL_2689

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
MUL_2682	2	17	-1.924078	RNA polymerase sigma factor SigF
MUL_2683	3	19	-2.124747	anti-sigma factor RsbW
MUL_2685	3	23	-3.762311	hypothetical protein
MUL_2686	1	19	-4.076642	hypothetical protein
MUL_2688	1	15	-4.106604	hypothetical protein
MUL_2689	1	14	-3.564092	L-lysine aminotransferase

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_2682	THERMOLYSIN	28	0.043	Thermolysin metalloprotease (M4) family signature.
>THERMOLYSIN#Thermolysin metalloprotease (M4) family signature. Length = 544 Score = 28.4 bits (63), Expect = 0.043 Identities = 32/154 (20%), Positives = 46/154 (29%), Gaps = 30/154 (19%) Query: 35 NLINPGPASYRPASFGHKGQVYDGWETRRRREPGHDQAIVRLGVPGVIRGVVVDTAWFKG 94 + P P ++ G+V + W +PG Q + GV RGV+ D + Sbjct: 188 RFLTPVPGNWIYMIDAADGKVLNKWNQMDEAKPGGAQPVAGTSTVGVGRGVLGDQKYINT 247 Query: 95 NYPPEVSIEALAVDGYPPAEQLASDGGWETIVAR------AKVVGDADNPFVVSSEK--- 145 Y + GY + G T R + D DN F S + Sbjct: 248 TYS--------SYYGYYYLQDNTRGSGIFTYDGRNRTVLPGSLWADGDNQFFASYDAAAV 299 Query: 146 -------------RWTHVRLSIYPDGGVARLRVH 166 + H RLS R VH Sbjct: 300 DAHYYAGVVYDYYKNVHGRLSYDGSNAAIRSTVH 333

MUL_5105

MUL_2858

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
MUL_5105	-2	16	-4.061169	transcriptional regulatory protein
MUL_2839	-1	16	-4.102400	short chain dehydrogenase
MUL_2840	0	16	-3.404521	hypothetical protein
MUL_2841	0	17	-3.395540	rifampin ADP-ribosyl transferase
MUL_2842	0	20	-3.858053	quinone reductase, Qor
MUL_2843	1	18	-3.645570	amidase
MUL_2844	0	11	0.273715	membrane-associated phospholipase C2 PlcB
MUL_2846	0	11	1.620689	hydrolase
MUL_2847	-1	10	2.042111	thiazole synthase
MUL_2848	-1	13	4.056230	thiamine biosynthesis protein ThiS
MUL_2849	-2	13	3.817607	thiamine biosynthesis oxidoreductase ThiO
MUL_2850	-1	13	2.598695	thiamine-phosphate pyrophosphorylase
MUL_2851	1	18	2.420888	mutator protein MutT3
MUL_2852	1	18	1.864808	hypothetical protein
MUL_2853	1	18	1.564694	glutamine-binding lipoprotein GlnH
MUL_2854	1	18	-2.810015	serine/threonine-protein kinase PknG
MUL_2855	3	21	-2.908095	hypothetical protein
MUL_2856	2	20	-2.155980	acetate kinase
MUL_2857	2	17	-1.096357	F420-dependent glucose-6-phosphate dehydrogenase
MUL_2858	2	18	-1.035406	beta lactamase-like protein

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_2842	PF04335	31	0.005	VirB8 type IV secretion protein
>PF04335#VirB8 type IV secretion protein Length = 227 Score = 30.6 bits (69), Expect = 0.005 Identities = 13/68 (19%), Positives = 23/68 (33%) Query: 122 ALKQRNWGWVLAIVVIVLALAAIAILGTVLLTRNKHPNVSQEDRVRQTIQHFDAAVQTGD 181 A + + WV+A V LA A + + + + P V DR Sbjct: 28 AERSKKLAWVVAGVAGALATAGVVAVAALTPLKTVEPYVITVDRNTGEASIAAKLHGDAT 87 Query: 182 LTALRSIT 189 +T ++ Sbjct: 88 ITYDEAVR 95
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_2851	NUCEPIMERASE	34	2e-04	Nucleotide sugar epimerase signature.
>NUCEPIMERASE#Nucleotide sugar epimerase signature. Length = 334 Score = 34.4 bits (79), Expect = 2e-04 Identities = 20/71 (28%), Positives = 32/71 (45%), Gaps = 12/71 (16%) Query: 60 GRLGRHLAAA----GHRVVGVDV-----DPALIEA--AEQDYPGPQWLVGDLAELDLPAR 108 G +G H++ GH+VVG+D D +L +A PG Q+ DLA+ + Sbjct: 10 GFIGFHVSKRLLEAGHQVVGIDNLNDYYDVSLKQARLELLAQPGFQFHKIDLADREGMTD 69 Query: 109 GIAE-PFDVIV 118 A F+ + Sbjct: 70 LFASGHFERVF 80
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_2853	cloacin	39	4e-05	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 38.5 bits (89), Expect = 4e-05 Identities = 32/102 (31%), Positives = 38/102 (37%) Query: 219 GNGGAGGDGGAGGVGGDGGAGGVGGVGGDGGAGGVGGVGGDGGWLIGDGGAGGQGGVGGM 278 G G G + GA G+ G G G G + G G + W G G GG G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 279 GGTGGAGGSGVAGAHGGNATSAVAAFGGDGGAGGDAGHGGTG 320 G GG G SG GGN ++ A A G GG Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 37.8 bits (87), Expect = 7e-05 Identities = 30/79 (37%), Positives = 36/79 (45%), Gaps = 2/79 (2%) Query: 163 GNAGLIGNGGAGGNGGA--GGNGGAGAAGGTGDNGGWLYGSGGDGGTGGNALVAGGTGGN 220 G G N GA G GG G G GG D GW + GG G+ + GG G+ Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 221 GGAGGDGGAGGVGGDGGAG 239 G GG+G +GG G GG Sbjct: 63 GNGGGNGNSGGGSGTGGNL 81 Score = 35.8 bits (82), Expect = 2e-04 Identities = 28/71 (39%), Positives = 36/71 (50%), Gaps = 1/71 (1%) Query: 125 GAAGTATNPNGGAGGLLYGNGGAGFNNGATAGAAGGNGGNAGLIGNGGAGGNGGAGGNGG 184 GA T+ N NGG GL G G + + ++ G G +G I GG G+G GGNG Sbjct: 12 GAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSG-IHWGGGSGHGNGGGNGN 70 Query: 185 AGAAGGTGDNG 195 +G GTG N Sbjct: 71 SGGGSGTGGNL 81 Score = 35.5 bits (81), Expect = 4e-04 Identities = 33/82 (40%), Positives = 38/82 (46%), Gaps = 2/82 (2%) Query: 143 GNGGAGFNNGATAGAAGGNGGNAGLIGNGGAGGNGGAGGNGGAGAAGGTGDNGGWLYGSG 202 G G G N GA + + NGG GL GGA G GG+G W GSG Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWG-GGSGSGIHWGGGSG 61 Query: 203 -GDGGTGGNALVAGGTGGNGGA 223 G+GG GN+ GTGGN A Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSA 83 Score = 33.5 bits (76), Expect = 0.001 Identities = 31/100 (31%), Positives = 37/100 (37%), Gaps = 5/100 (5%) Query: 159 GGNGGNAGLIGNGGAGGNGGAGGNGGAGAAGGTGDNGGWLYGSGGDGGTGGNALVAGGTG 218 G N G GN G G G G + +G + +N W GSG GG G Sbjct: 8 GHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGG-----GSGH 62 Query: 219 GNGGAGGDGGAGGVGGDGGAGGVGGVGGDGGAGGVGGVGG 258 GNGG G+ G G G + V A G GG Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG 102 Score = 33.1 bits (75), Expect = 0.002 Identities = 29/101 (28%), Positives = 36/101 (35%) Query: 266 DGGAGGQGGVGGMGGTGGAGGSGVAGAHGGNATSAVAAFGGDGGAGGDAGHGGTGGDGGN 325 + GA G G TG G G + G ++ + G G G G G G Sbjct: 10 NTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNG 69 Query: 326 GGQGASGGRGGLLSGAQGVTGTAGDGGTGGDGGLHGAFGAG 366 G SG G L + A V T G GGL + AG Sbjct: 70 NSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAG 110 Score = 31.2 bits (70), Expect = 0.008 Identities = 35/115 (30%), Positives = 45/115 (39%), Gaps = 4/115 (3%) Query: 190 GTGDNGGWLYGSGG-DGGTGGNALVAGGTGGNGGAGGDGGAGGVGGDGGAGGVGGVGGDG 248 G G N G SG +GG G + G + G+G + + GG G G G G G+G Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNG 65 Query: 249 GAGGVGGVGGDGGWLIGDGGAGGQGGVGGMGGTGGAGGSGVAGAHGGNATSAVAA 303 G G G G G G+ A G G G+A + A SA A Sbjct: 66 GGNGNSGGGSGTG---GNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAAIA 117
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_2855	IGASERPTASE	30	0.016	IgA-specific serine endopeptidase (S6) signature.
>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature. Length = 1541 Score = 30.0 bits (67), Expect = 0.016 Identities = 17/51 (33%), Positives = 21/51 (41%), Gaps = 3/51 (5%) Query: 283 VTFDEDRHRAHTGNGAQVLATLRNTAINLHRLNGADNIADACRITALTANR 333 V E+ H TGN L N I+ LN ADN + + LT N Sbjct: 846 VRLTENSHWHLTGNSDVHQLDLANGHIH---LNSADNSNNVTKYNTLTVNS 893
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_2856	TCRTETA	29	0.003	Tetracycline resistance protein signature.
>TCRTETA#Tetracycline resistance protein signature. Length = 399 Score = 29.0 bits (65), Expect = 0.003 Identities = 12/100 (12%), Positives = 33/100 (33%), Gaps = 4/100 (4%) Query: 1 MPTTTPPRRRPTEGIAQMNIVPASIEEKISRVDRQRSLAIGISCGVLAAWSFFRLVWLLY 60 +P + RRP + + + +R + + + + +W+++ Sbjct: 181 LPESHKGERRP----LRREALNPLASFRWARGMTVVAALMAVFFIMQLVGQVPAALWVIF 236 Query: 61 VSMPFGWFMGAVAFQFVLWAVVGSVATNAAAGFLARYFKD 100 F W + + ++ S+A G +A + Sbjct: 237 GEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGE 276
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_2857	TCRTETOQM	26	0.014	Tetracycline resistance protein TetO/TetQ/TetM family ...
>TCRTETOQM#Tetracycline resistance protein TetO/TetQ/TetM family signature. Length = 639 Score = 26.4 bits (58), Expect = 0.014 Identities = 11/44 (25%), Positives = 22/44 (50%), Gaps = 4/44 (9%) Query: 22 RVEHGELRVGDEVRINDGSGVRVDAIEAF----RKKLDTAKAGD 61 R+ G L + D VRI++ +++ + K+D A +G+ Sbjct: 269 RLYSGVLHLRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGE 312

MUL_2894

MUL_2910

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
MUL_2894	-2	19	-4.168993	putative regultory protein
MUL_2895	-2	18	-3.461449	methyltransferase
MUL_2896	-1	19	-3.469500	tRNA/rRNA methyltransferase SpoU
MUL_2898	-2	18	-3.182275	PE-PGRS family protein
MUL_5111	-2	18	-2.192314	non-IS element not present in Mycobacterium
MUL_2901	-2	16	-1.943730	nitroreductase
MUL_2902	-1	14	-0.894440	transposase for IS2404
MUL_2904	1	18	-2.258873	hypothetical protein
MUL_2905	2	13	-2.105225	iron-regulated elongation factor Tu Tuf-like
MUL_2906	1	13	-2.660360	hypothetical protein
MUL_2907	1	13	-3.573842	transposase for IS2404
MUL_2909	0	13	-2.829206	hypothetical protein
MUL_2910	-1	15	-3.472248	oxidoreductase

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_5111	TYPE3OMGPROT	35	5e-05	Type III secretion system outer membrane G protein ...
>TYPE3OMGPROT#Type III secretion system outer membrane G protein family signature. Length = 607 Score = 34.9 bits (80), Expect = 5e-05 Identities = 29/119 (24%), Positives = 48/119 (40%), Gaps = 17/119 (14%) Query: 7 TAQSAVSKVIIEALESATATSVISALAEGRNTASKNIFRPSIMTLE----TIDGQEIRVS 62 + + S V L+ A ++ L E +A + RP+++T E ID E Sbjct: 328 SNGALGSLVDARGLDYLLAR--VNLL-ENEGSAQV-VSRPTLLTQENAQAVIDHSETYYV 383 Query: 63 TLTSKSATAAELVSAGTTIDVTPTGTYVLACGKT------LVIENGRLVGGTAAAGDIP 115 +T K + ++ GT + +TP VL G L IE+G ++ IP Sbjct: 384 KVTGKEVAELKGITYGTMLRMTPR---VLTQGDKSEISLNLHIEDGNQKPNSSGIEGIP 439
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_2909	PF05272	30	0.014	Virulence-associated E family protein
>PF05272#Virulence-associated E family protein Length = 892 Score = 29.7 bits (66), Expect = 0.014 Identities = 16/43 (37%), Positives = 19/43 (44%) Query: 22 PVTPPPLPRPVTFDQRWSDLTFVHWPVLPDSVAHMYPPGTRPD 64 P P P PRPV + W + V +P S H P G PD Sbjct: 118 PPRPEPPPRPVVEKECWETIQPVPEHAVPPSFWHPAPKGREPD 160

MUL_2978

MUL_2995

Genomic Island

LocusTag	DNBias	CDNBias	%GCBias	Product
MUL_2978	-1	18	-3.492006	hypothetical protein
MUL_2979	0	19	-3.781774	PPE family protein
MUL_2980	1	21	-4.151167	TetR family transcriptional regulator
MUL_2982	1	19	-4.269730	dehydrogenase
MUL_2983	1	18	-3.863286	oxidoreductase FadB5
MUL_2984	3	18	-3.659446	hypothetical protein
MUL_2988	3	18	-3.024309	ArsR-type repressor
MUL_2992	2	19	-2.127864	hypothetical protein
MUL_2993	1	16	-1.680882	hypothetical protein
MUL_2994	1	16	-1.025261	hypothetical protein
MUL_2995	2	14	-1.328290	transposase for IS2404

MUL_3055

MUL_3086

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
MUL_3055	3	10	1.704023	hypothetical protein
MUL_3056	5	9	1.643945	alanine and proline rich secreted protein Apa
MUL_3059	3	11	-0.107275	molybdenum-transport ABC transporter ATP-binding
MUL_3060	3	12	0.194244	hypothetical protein
MUL_3061	4	9	0.751867	hypothetical protein
MUL_3062	3	8	1.517211	O-methyltransferase Omt
MUL_3063	-1	10	-2.033572	molybdenum-transport integral membrane protein
MUL_3065	1	12	-2.236452	molybdate-binding lipoprotein ModA
MUL_3066	1	11	-1.005037	short chain dehydrogenase
MUL_3067	1	13	0.409084	oxidoreductase
MUL_3068	2	13	0.746171	NADH dehydrogenase Ndh
MUL_3069	4	15	0.588580	urease accessory protein UreD
MUL_3070	4	13	-0.363583	urease accessory protein UreG
MUL_3071	3	14	-0.235013	urease accessory protein UreF
MUL_3072	1	16	-0.353005	urease subunit alpha
MUL_3074	0	16	-0.750672	urease subunit beta UreB
MUL_3076	-1	14	-1.315780	urease subunit gamma
MUL_3077	-1	13	-1.609363	hypothetical protein
MUL_3078	0	15	-0.901348	transcriptional regulatory protein
MUL_3079	0	15	-0.875435	hypothetical protein
MUL_3080	1	14	-2.036471	6-phosphogluconate dehydrogenase
MUL_3081	2	18	-3.312802	inosine 5-monophosphate dehydrogenase
MUL_3082	4	18	-2.579725	hypothetical protein
MUL_3083	4	17	-2.335558	preprotein translocase subunit SecA
MUL_3084	4	17	-1.949946	CDP-diacylglycerol--glycerol-3-phosphate
MUL_3085	3	17	-2.272981	hypothetical protein
MUL_3086	2	17	-2.180573	hypothetical protein

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_3056	cloacin	36	4e-04	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 35.8 bits (82), Expect = 4e-04 Identities = 39/123 (31%), Positives = 44/123 (35%), Gaps = 13/123 (10%) Query: 144 GNGGIGQAGGAGGDAGLIGNGGSGGIGGPDSTGLPGGAGGAGGWLLGDGGNGGIGGAGTG 203 G G G GA +G I NGG G+G GGA GW + GG G+G Sbjct: 3 GGDGRGHNTGAHSTSGNI-NGGPTGLGVG------GGASDGSGWSSENNPWGGGSGSGIH 55 Query: 204 IGGAGGSGGQGGAAAWLFGSGGTGGAGGFGNSGTGGPGGAGGTGGLLIGNGGNGGYDIQG 263 GG G G GG GG+G GN A G L G I Sbjct: 56 WGGGSGHGNGGG------NGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISA 109 Query: 264 GAF 266 GA Sbjct: 110 GAL 112 Score = 35.5 bits (81), Expect = 5e-04 Identities = 31/90 (34%), Positives = 37/90 (41%), Gaps = 5/90 (5%) Query: 233 GNSGTGGPGGAGGTGGLLIGNGGNGGYDIQGGAFGGDGGNARLIGNGGDGGVEAGGPGTT 292 G G G GA T G + NGG G + GGA G G ++ N GG G Sbjct: 3 GGDGRGHNTGAHSTSGNI--NGGPTGLGVGGGASDGSGWSSE---NNPWGGGSGSGIHWG 57 Query: 293 GGDGGNGGWLLGNGGNGAADGGGAGSAAGA 322 GG G G GN G G+ GG + A Sbjct: 58 GGSGHGNGGGNGNSGGGSGTGGNLSAVAAP 87 Score = 34.7 bits (79), Expect = 0.001 Identities = 40/108 (37%), Positives = 46/108 (42%), Gaps = 6/108 (5%) Query: 138 GNGGSGGNGGIGQAGGA--GGDAGLIGNGGSGGIGGPDSTGLP--GGAGGAGGWLLGDG- 192 G G G N G G GG GL GG+ G S P GG+G W G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 193 GNGGIGGAGTGIGGAGGSGGQGGAA-AWLFGSGGTGGAGGFGNSGTGG 239 GNGG G G G GG+ A A+ F + T GAGG S + G Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAG 110 Score = 34.7 bits (79), Expect = 0.001 Identities = 33/99 (33%), Positives = 38/99 (38%), Gaps = 8/99 (8%) Query: 169 IGGPDSTGLPGGAGGAGGWLLGDGGNGGIGGAGTGIGGAGGSGGQGGAAAWLFGSGGTGG 228 + G D G GA G + NGG G G G G + GSG W GG+G Sbjct: 1 MSGGDGRGHNTGAHSTSGNI-----NGGPTGLGVGGGASDGSGWSSENNPW---GGGSGS 52 Query: 229 AGGFGNSGTGGPGGAGGTGGLLIGNGGNGGYDIQGGAFG 267 +G G GG G G G GGN AFG Sbjct: 53 GIHWGGGSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFG 91 Score = 32.0 bits (72), Expect = 0.006 Identities = 35/121 (28%), Positives = 39/121 (32%), Gaps = 5/121 (4%) Query: 360 GSGGNGGFNGYQGVGGAGGAGGKLFGDGGDGGDCAESNGGIGSGGRGGSAVGLIGNGGAG 419 G G N G + G G G + G DG + N G G G G G G Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNG 65 Query: 420 GVGGVGRNANAGGSGGSGGNAAWLFG-----DGGAGGSGGSSDTAASGAGGNGGTAALIG 474 G G + G S A FG GAGG S A A AAL G Sbjct: 66 GGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAAIADIMAALKG 125 Query: 475 N 475 Sbjct: 126 P 126 Score = 31.6 bits (71), Expect = 0.009 Identities = 32/102 (31%), Positives = 39/102 (38%), Gaps = 3/102 (2%) Query: 397 NGGIGSGGRGGSAVGLIGNGGAGGVGGVGRNANAGGSGGSGGNAAWLFGDGGAGGSGGSS 456 +GG G G G A GN G G + GSG S N W G G GG S Sbjct: 2 SGGDGRGHNTG-AHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGS 60 Query: 457 DTAASGAGGNGGTAALIGNGGAGGDAGVSFA--GMAGPGGNG 496 G GN G + G + A V+F ++ PG G Sbjct: 61 GHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG 102 Score = 30.5 bits (68), Expect = 0.022 Identities = 30/82 (36%), Positives = 36/82 (43%), Gaps = 7/82 (8%) Query: 305 NGGNGAADGGGAGSAAGA--GGNAGLIGNGGHG-----GLEHIDGTGGAGSIAGAGGDAG 357 +GG+G GA S +G GG GL GG E+ GG+GS GG +G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 358 LFGSGGNGGFNGYQGVGGAGGA 379 GGNG G G GG A Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSA 83 Score = 29.7 bits (66), Expect = 0.037 Identities = 26/76 (34%), Positives = 32/76 (42%), Gaps = 2/76 (2%) Query: 449 AGGSGGSSDTAASGAGGN--GGTAALIGNGGAGGDAGVSFAGMAGPGGNGGDARLIGDGG 506 +GG G +T A GN GG L GGA +G S GG+G G G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 507 DGGRGAPGGAGGMGGS 522 G G G +GG G+ Sbjct: 62 HGNGGGNGNSGGGSGT 77 Score = 29.3 bits (65), Expect = 0.045 Identities = 30/92 (32%), Positives = 35/92 (38%), Gaps = 6/92 (6%) Query: 261 IQGGAFGGDGGNARLIGNGGDGGVEAGGPGTTGGDGGNGGWLLGNGGNGAADGGGAGSAA 320 + GG G A +GG G G DG GW N GGG+GS Sbjct: 1 MSGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDG--SGWSSENNPW----GGGSGSGI 54 Query: 321 GAGGNAGLIGNGGHGGLEHIDGTGGAGSIAGA 352 GG +G GG+G GTGG S A Sbjct: 55 HWGGGSGHGNGGGNGNSGGGSGTGGNLSAVAA 86
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_3059	PF05272	34	0.003	Virulence-associated E family protein
>PF05272#Virulence-associated E family protein Length = 892 Score = 33.5 bits (76), Expect = 0.003 Identities = 12/52 (23%), Positives = 23/52 (44%) Query: 456 LVITGRSGSGKTTLLRSLAELWPFASGTLSRPDGANDTMFLSQLPYVPLGSL 507 +V+ G G GK+TL+ +L L F+ G + ++ + L + Sbjct: 599 VVLEGTGGIGKSTLINTLVGLDFFSDTHFDIGTGKDSYEQIAGIVAYELSEM 650
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_3062	cloacin	37	3e-04	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 37.0 bits (85), Expect = 3e-04 Identities = 34/99 (34%), Positives = 42/99 (42%), Gaps = 3/99 (3%) Query: 376 GGGGAGGAGGFGTISDGGAGGRGGGGGQLLGNGGAGGAGGQGGNDGGAGGLGGNGVLIGN 435 GG G G G + S GG G G G G + G+G N+ GG G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGV---GGGASDGSGWSSENNPWGGGSGSGIHWGGG 59 Query: 436 GGNGGVGGVGETPGGDGGGGISGLLLGADGFNAPAGSSP 474 G+G GG G + GG G GG + F PA S+P Sbjct: 60 SGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTP 98 Score = 35.1 bits (80), Expect = 0.001 Identities = 34/107 (31%), Positives = 41/107 (38%), Gaps = 5/107 (4%) Query: 321 GAGGAGGHAFDGDGGAGGAGGNAGLMFSSGGSGGVGGAGSIDGGAGGAGGDAGWLGGGGA 380 G G G + GG GL G S G G + + GG+G W GG G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 381 GGAGGFGTISDGGAGGRGGGGGQLLGNGGAGGAGGQGGNDGGAGGLG 427 G GG +G +GG G GG L G + GAGGL Sbjct: 63 GNGGG-----NGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 34.7 bits (79), Expect = 0.002 Identities = 33/108 (30%), Positives = 40/108 (37%), Gaps = 3/108 (2%) Query: 521 GDGGAGGSAAATPGAAGGAGGAAGLVGAGGAGGAGANTGISNPGFGGAGGAGGAGAGAGG 580 G G+ P G GGA+ G+G + G S G GG+G G G Sbjct: 12 GAHSTSGNINGGPTGLGVGGGASD--GSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNG 69 Query: 581 GVGEAGGAGGLLGAGGTGGGGGVAPSLVDGGTGGAGGAGGAGGLFGGI 628 G G GG L A G P+L G GG + AG L I Sbjct: 70 NSGGGSGTGGNLSAVAAPVAFGF-PALSTPGAGGLAVSISAGALSAAI 116 Score = 34.3 bits (78), Expect = 0.002 Identities = 29/86 (33%), Positives = 35/86 (40%), Gaps = 4/86 (4%) Query: 717 IGGAGGRGGNAGLLFS----DAGVGGFGGFGGTGGNAGWLGSGGSGGAGGGSNGDGGAGG 772 + G GRG N G + + G G G GG +GW G G GS G G Sbjct: 1 MSGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGS 60 Query: 773 TGGSGGQIVGDGGAGGAGGQGDLLAA 798 G+GG GG G GG +AA Sbjct: 61 GHGNGGGNGNSGGGSGTGGNLSAVAA 86 Score = 34.3 bits (78), Expect = 0.002 Identities = 21/60 (35%), Positives = 26/60 (43%) Query: 712 GTSTGIGGAGGRGGNAGLLFSDAGVGGFGGFGGTGGNAGWLGSGGSGGAGGGSNGDGGAG 771 G TG+G GG +G + GG G G G G+GG G GG +G GG Sbjct: 22 GGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNGNSGGGSGTGGNL 81 Score = 34.3 bits (78), Expect = 0.002 Identities = 38/111 (34%), Positives = 44/111 (39%), Gaps = 5/111 (4%) Query: 229 GAGGLGGVGGSGNVGGNGGAGGAGGLLAGLVGAGGGDGGSGGLGAAGGDGGNGGRAGLFG 288 G G G G+ + GN G G + G GA G G S GG G+G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGG--GASDGSGWSSENNPWGGGSGSGIHWGGGS 60 Query: 289 GAGGAGGMGATGGHSGGDGGAGGDAGLL---FGTGGAGGAGGHAFDGDGGA 336 G G GG G +GG SG G A + F GAGG A GA Sbjct: 61 GHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGA 111 Score = 33.9 bits (77), Expect = 0.003 Identities = 35/109 (32%), Positives = 41/109 (37%), Gaps = 7/109 (6%) Query: 735 GVGGFGGFGGTGGNAGWLGSGGSGGAGGGSNGDGGA------GGTGGSGGQIVGDGGAGG 788 G G G T GN G G G GG S+G G + GG GSG G G G Sbjct: 6 GRGHNTGAHSTSGNING-GPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGN 64 Query: 789 AGGQGDLLAAGGSGGDGGQGGDAVLIGTGGNGGNGASGVLAGIGGDGGS 837 GG G+ G+GG+ V G GA G+ I S Sbjct: 65 GGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALS 113 Score = 33.5 bits (76), Expect = 0.004 Identities = 27/81 (33%), Positives = 28/81 (34%) Query: 346 MFSSGGSGGVGGAGSIDGGAGGAGGDAGWLGGGGAGGAGGFGTISDGGAGGRGGGGGQLL 405 M G G GA S G G G GG G GG G G G Sbjct: 1 MSGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGS 60 Query: 406 GNGGAGGAGGQGGNDGGAGGL 426 G+G GG G GG G G L Sbjct: 61 GHGNGGGNGNSGGGSGTGGNL 81 Score = 33.5 bits (76), Expect = 0.004 Identities = 32/86 (37%), Positives = 39/86 (45%), Gaps = 5/86 (5%) Query: 171 GAGGAGGNSPLRNTANGGNGGTGGAGGLLFGPGGVGGAGGASFLTGGTGGDGGAGGLFGA 230 G G G N+ +T+ NGG G G G G G+G +S GG G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGV---GGGASDGSGWSSENNPWGGGSGSGIHWGGG 59 Query: 231 GGLGGVGGSGNVGGNGGAGGAGGLLA 256 G G GG+GN GG G+G G L A Sbjct: 60 SGHGNGGGNGNSGG--GSGTGGNLSA 83 Score = 33.1 bits (75), Expect = 0.005 Identities = 35/104 (33%), Positives = 44/104 (42%), Gaps = 8/104 (7%) Query: 300 GGHSGGDGGAGGDAGLLFGTGGAGGAGGHAFDGDGGAG-----GAGGNAGLMFSSGGSGG 354 G G + GA +G + G G GG A DG G + G G +G+ + G G Sbjct: 4 GDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHG 63 Query: 355 VGGAGSIDGGAGGAGGDAGWLGGGGAGGAGGFGTISDGGAGGRG 398 GG G +GG G G L A A GF +S GAGG Sbjct: 64 NGGGN---GNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 33.1 bits (75), Expect = 0.005 Identities = 35/100 (35%), Positives = 38/100 (38%), Gaps = 3/100 (3%) Query: 144 GNGGAGGSGAAGQAGGD--GGAAGLIGAGGAGGAGGNSPLRNTANGGNG-GTGGAGGLLF 200 G G G + A G+ GG GL GGA G S N GG+G G GG Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 201 GPGGVGGAGGASFLTGGTGGDGGAGGLFGAGGLGGVGGSG 240 G GG G G TGG A FG L G G Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG 102 Score = 32.4 bits (73), Expect = 0.009 Identities = 39/140 (27%), Positives = 51/140 (36%), Gaps = 7/140 (5%) Query: 571 AGGAGAGAGGGVGEAGG-AGGLLGAGGTGGGGGVAPSLVDGGTGGAGGAGGAGGLFGGIF 629 +GG G G G G G G GGG GG+G GG Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGG-- 59 Query: 630 GAGGGDGGAGGFTGGIDGAGGVGGAGGNTGLLGGPG-GSGGSGGDGIAKLGFNVDGGAGG 688 +G G+GG G +GG G GG A G P + G+GG ++ + Sbjct: 60 -SGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAAIAD 118 Query: 689 --AGVNGGFLFGSGGTGGFG 706 A + G F FG G +G Sbjct: 119 IMAALKGPFKFGLWGVALYG 138 Score = 32.0 bits (72), Expect = 0.013 Identities = 28/96 (29%), Positives = 35/96 (36%), Gaps = 5/96 (5%) Query: 534 GAAGGAGGAAGLVGAGGAGGAGANTGISNPGFGGAGGAGGAGAGAGGGVGEAGGAGGLLG 593 G GA +G + G G G+ G G+G+G G G G G Sbjct: 8 GHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGG 67 Query: 594 AGGTGGGGGVAPSLVDGGTGGAGG-----AGGAGGL 624 G +GGG G +L A G GAGGL Sbjct: 68 NGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGL 103 Score = 31.6 bits (71), Expect = 0.017 Identities = 28/82 (34%), Positives = 35/82 (42%), Gaps = 2/82 (2%) Query: 263 GGDGGSGGLGAAGGDGG-NGGRAGLFGGAGGAGGMGATGGHSGGDGGAGGDAGLLFGTGG 321 GGDG GA G NGG GL G G + G G + ++ GG+G G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHW-GGGSG 61 Query: 322 AGGAGGHAFDGDGGAGGAGGNA 343 G GG+ G G G +A Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSA 83 Score = 30.8 bits (69), Expect = 0.024 Identities = 32/84 (38%), Positives = 35/84 (41%), Gaps = 6/84 (7%) Query: 665 GGSGGSGGDGIAKLGFNVDGGAGGAGVNGGFLFGSGGTGGFGGVGGVGTSTGIGGAGGRG 724 GG G G N++GG G GV GG GSG + GG S G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 725 GNAGLLFSDAGVGGFGGFGGTGGN 748 GN G G G GG GTGGN Sbjct: 63 GNGG------GNGNSGGGSGTGGN 80 Score = 30.5 bits (68), Expect = 0.039 Identities = 29/130 (22%), Positives = 41/130 (31%), Gaps = 11/130 (8%) Query: 361 IDGGAGGAGGDAGWLGGGGAGGAGGFGTISDGGAGGRGGGGGQLLGNGGAGGAGGQGGND 420 + GG G G G + G + G G GG+G GG Sbjct: 1 MSGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGS 60 Query: 421 GGAGGLGGNGVLIGNGGNGGVGGVGETPGGDGGGGISGLLLGADGFNAPAGSSPVHALQQ 480 G GNGG G G G GG+ + + G + P ++ Sbjct: 61 GH-----------GNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISA 109 Query: 481 QALAAINAPV 490 AL+A A + Sbjct: 110 GALSAAIADI 119
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_3076	CHANLCOLICIN	29	0.045	Channel forming colicin signature.
>CHANLCOLICIN#Channel forming colicin signature. Length = 522 Score = 28.9 bits (64), Expect = 0.045 Identities = 30/99 (30%), Positives = 39/99 (39%), Gaps = 10/99 (10%) Query: 54 GDGWLGPASESMEAAVFPYLAW--MNITGMQAEQTARQAAAAAGAFEAAFAMTVPPAQVA 111 G G G SES AA+ W + QAEQ AR AAA +A A Sbjct: 37 GGGKGGSKSES-SAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAK-------ANRD 88 Query: 112 ANRTQLQTLVATNLLGQNTPAIAATEAAYGEMWAQDAAA 150 A +L+ +V L + +ATE A+ A A Sbjct: 89 ALTQRLKDIVNEALRHNASRTPSATELAHANNAAMQAED 127
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_3079	SUBTILISIN	116	9e-31	Subtilisin serine protease family (S8) signature.
>SUBTILISIN#Subtilisin serine protease family (S8) signature. Length = 326 Score = 116 bits (291), Expect = 9e-31 Identities = 49/265 (18%), Positives = 92/265 (34%), Gaps = 66/265 (24%) Query: 263 FSGIAPEVELISIRQSSQAFGLKDPYTGDEDPQTQQKIDDVETMARAIVHAANMGASVIN 322 G+APE +L+ I+ ++ + + + I +A +I+ Sbjct: 103 VVGVAPEADLLIIKVLNKQGS-----------------GQYDWIIQGIYYAIEQKVDIIS 145 Query: 323 ISDVTCMSARNVIDQNALGAAVHYAAVDKNVVIVAAAGDGSKKDCKQNPIFDPLQPDDPR 382 MS D L AV A V ++++ AAG+ Sbjct: 146 ------MSLGGPEDVPELHEAVKKA-VASQILVMCAAGNEG------------------D 180 Query: 383 DWNAVTTVVTPSWFSDYVLTVGAVDTNGQPMTKMSIAGPWVSIAAPGTDVIGLSPRDDGL 442 + + P + + V++VGA++ + S + V + APG D++ Sbjct: 181 GDDRTDELGYPGCY-NEVISVGAINFDRHASE-FSNSNNEVDLVAPGEDILS-------- 230 Query: 443 INAIDGPDNSLLVPAGTSFATAIVSGVVALVRAKYP-----ELSAYQIRNRLIHTARPPA 497 P +GTS AT V+G +AL++ +L+ ++ +LI P Sbjct: 231 ----TVPGGKYATFSGTSMATPHVAGALALIKQLANASFERDLTEPELYAQLIKRTIPLG 286 Query: 498 RGVDNQVGYGVVDPVAA----LTWD 518 G G++ A +D Sbjct: 287 -NSPKMEGNGLLYLTAVEELSRIFD 310 Score = 70.6 bits (173), Expect = 2e-15 Identities = 28/84 (33%), Positives = 38/84 (45%), Gaps = 6/84 (7%) Query: 77 TDFKLQPKYMEMLNINEAWQFGRGAGVKVAVIDTGVTP-HPRFP-HLIPGGDYVMGGDG- 133 P+ +EM+ W RG GVKVAV+DTG HP +I G ++ +G Sbjct: 17 QQVNEIPRGVEMIQAPAVWNQTRGRGVKVAVLDTGCDADHPDLKARIIGGRNFTDDDEGD 76 Query: 134 ---LQDCDAHGTIVASMIGAAPAN 154 +D + HGT VA I A Sbjct: 77 PEIFKDYNGHGTHVAGTIAATENE 100

MUL_3161

MUL_3169

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
MUL_3161	3	11	-0.708309	short-chain type dehydrogenase/reductase
MUL_3162	3	11	-0.516071	hypothetical protein
MUL_3166	3	12	-0.479877	hypothetical protein
MUL_3167	3	12	-0.528807	AcrR family transcriptional regulator
MUL_3168	4	12	-0.210364	hypothetical protein
MUL_3169	4	10	-0.327835	methyltransferase

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_3162	CHANLCOLICIN	29	0.030	Channel forming colicin signature.
>CHANLCOLICIN#Channel forming colicin signature. Length = 522 Score = 29.3 bits (65), Expect = 0.030 Identities = 22/77 (28%), Positives = 37/77 (48%), Gaps = 7/77 (9%) Query: 144 ASPTLLNHTAKSAARAYANMELPLAEVKAVAKATDTSINDVVMTIVDDALHHYLDEHRAP 203 ++ L A+ AARA A AE +A AKA ++ + IV++AL H + R P Sbjct: 58 STAQLKKTQAEQAARAKA-----AAEAQAKAKANRDALTQRLKDIVNEALRH--NASRTP 110 Query: 204 ADGPLVALMPMSMRSQA 220 + L +M+++ Sbjct: 111 SATELAHANNAAMQAED 127
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_3168	DHBDHDRGNASE	107	6e-30	2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase signature. Length = 261 Score = 107 bits (267), Expect = 6e-30 Identities = 70/250 (28%), Positives = 113/250 (45%), Gaps = 14/250 (5%) Query: 2 AVVAGGAGGIGAATSRLFAQHGAQVVIADIDAELAHRTVDEIGGAAWV---VGTDVRDAD 58 A + G A GIG A +R A GA + D + E + V + A DVRD+ Sbjct: 11 AFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHAEAFPADVRDSA 70 Query: 59 QVSALAQRVLDRYGRLDILVNNVGHWLRHPGNFVDTDPQLWDELYRVNLHHVLLATHAFL 118 + + R+ G +DILVN G + PG + W+ + VN V A+ + Sbjct: 71 AIDEITARIEREMGPIDILVNVAG--VLRPGLIHSLSDEEWEATFSVNSTGVFNASRSVS 128 Query: 119 PAMIEQHGGAIVNVSSVEGLRGYPEDPVYAAFKAAVIHFTHSLAVQVGNHGVRINAIAPD 178 M+++ G+IV V S YA+ KAA + FT L +++ + +R N ++P Sbjct: 129 KYMMDRRSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLELAEYNIRCNIVSPG 188 Query: 179 VTESLQVPYSQWLSD--AEQT------QWPGWVPVGRMGVPEDQARVILFLACELSAFVT 230 TE+ + +S W + AEQ + +P+ ++ P D A +LFL + +T Sbjct: 189 STET-DMQWSLWADENGAEQVIKGSLETFKTGIPLKKLAKPSDIADAVLFLVSGQAGHIT 247 Query: 231 GHTIPTDGGT 240 H + DGG Sbjct: 248 MHNLCVDGGA 257

MUL_3200

MUL_3247

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
MUL_3200	2	11	-0.415039	hypothetical protein
MUL_3201	0	11	-0.568829	hypothetical protein
MUL_3202	0	12	-0.644957	hypothetical protein
MUL_3203	1	12	-0.951575	short-chain dehydrogenase
MUL_3204	0	13	-1.307904	hypothetical protein
MUL_5114	1	19	-0.954006	hypothetical protein
MUL_3206	0	20	-2.626074	hypothetical protein
MUL_3207	0	18	-3.080793	divalent cation-transport integral membrane
MUL_3208	1	20	-3.788766	short chain dehydrogenase
MUL_3210	1	28	-4.263185	PPE family protein
MUL_5090	3	29	-4.691108	hypothetical protein
MUL_3212	4	29	-5.276543	Z-decaprenyl diphosphate synthase
MUL_3213	4	28	-5.083904	hypothetical protein
MUL_3214	4	28	-5.068486	NADH dehydrogenase Ndh1
MUL_3215	2	23	-4.869285	hypothetical protein
MUL_3216	2	21	-4.670841	transcriptional accessory protein Tex
MUL_3217	-1	20	-4.665552	TRK system potassium uptake protein CeoC1
MUL_3218	-1	21	-4.023580	potassium/proton antiporter
MUL_3220	-1	21	-4.592890	hypothetical protein
MUL_3221	-1	16	-3.217325	transposase for IS2404
MUL_3224	-1	12	-2.475899	Ser/Thr protein kinase
MUL_3226	-1	10	-1.956132	hypothetical protein
MUL_3229	0	9	-1.376649	amino acid transporter PotE
MUL_3230	1	11	-0.676382	hypothetical protein
MUL_3232	1	16	1.429603	PE-PGRS family protein
MUL_3233	2	16	-0.093021	sugar transporter
MUL_3234	0	10	-1.336779	hypothetical protein
MUL_3235	1	11	-1.911714	hypothetical protein
MUL_3236	-1	11	-2.879197	succinate-semialdehyde dehydrogenase
MUL_3237	0	13	-3.481655	hydrolase/amidase
MUL_3238	-1	17	-3.456918	hypothetical protein
MUL_3239	-1	15	-2.697259	hypothetical protein
MUL_3240	2	16	1.171597	hydrolase
MUL_3241	2	13	1.573568	hypothetical protein
MUL_3243	1	13	2.511211	polyprenyl synthetase IdsB
MUL_3245	1	12	2.794780	translation initiation inhibitor
MUL_3246	1	13	3.851515	hypothetical protein
MUL_3247	1	12	3.741447	hypothetical protein

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_3243	PERTACTIN	27	0.035	Pertactin signature.
>PERTACTIN#Pertactin signature. Length = 922 Score = 27.4 bits (60), Expect = 0.035 Identities = 16/49 (32%), Positives = 22/49 (44%) Query: 61 HIATLIGYTRGDGGFQWENAMGDLAIGVVGIMAYWFRGHFWLATIVVLS 109 H+ L GYTRGD GF + ++ V G Y F+L + S Sbjct: 703 HLGGLAGYTRGDRGFTGDGGGHTDSVHVGGYATYIANSGFYLDATLRAS 751
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_3247	cloacin	35	5e-04	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 35.5 bits (81), Expect = 5e-04 Identities = 28/85 (32%), Positives = 32/85 (37%), Gaps = 3/85 (3%) Query: 394 MFGNGGAGGAGGDRVAGSQGNGGDGGDGGHGGTYFGSGGAGGH---GGYDDSGSGGQGGH 450 M G G G G NGG G G GG GSG + + GG SG GG Sbjct: 1 MSGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGS 60 Query: 451 GGDGGAAGTIGNGGDGGTGGDALVS 475 G G GG G G + V+ Sbjct: 61 GHGNGGGNGNSGGGSGTGGNLSAVA 85 Score = 35.1 bits (80), Expect = 8e-04 Identities = 28/92 (30%), Positives = 36/92 (39%) Query: 244 GGAGGAGGLLVGAGGHGGTGGTSASGTGATGGSGGAGGLLFSPGGAGGDGGAGFSGADGG 303 GA G + G G GG ++ G+G + + GG S GG G G G +G Sbjct: 11 TGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNGN 70 Query: 304 AGGNGGAGGGGLWFGNGGAGGIGGFDAHGDGG 335 +GG G GG A G G GG Sbjct: 71 SGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG 102 Score = 33.1 bits (75), Expect = 0.003 Identities = 27/77 (35%), Positives = 30/77 (38%), Gaps = 1/77 (1%) Query: 382 NGGAGGTGGDA-GMFGNGGAGGAGGDRVAGSQGNGGDGGDGGHGGTYFGSGGAGGHGGYD 440 N GA T G+ G G GG D S N GG G G + G G G GG Sbjct: 10 NTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNG 69 Query: 441 DSGSGGQGGHGGDGGAA 457 +SG G G AA Sbjct: 70 NSGGGSGTGGNLSAVAA 86 Score = 32.4 bits (73), Expect = 0.005 Identities = 25/84 (29%), Positives = 32/84 (38%) Query: 442 SGSGGQGGHGGDGGAAGTIGNGGDGGTGGDALVSGGTGGDGGDGGDGGDAREIGNGGNGG 501 SG G+G + G +G I G G G G + GG I GG G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 502 NAGAGATAGNEGTGGTGGQLIGVS 525 + G + G GTGG L V+ Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVA 85 Score = 32.4 bits (73), Expect = 0.005 Identities = 27/94 (28%), Positives = 30/94 (31%) Query: 287 GGAGGDGGAGFSGADGGAGGNGGAGGGGLWFGNGGAGGIGGFDAHGDGGDGGAGGNAGIY 346 GA G G G G G + G G N GG G H GG G G Sbjct: 11 TGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNGN 70 Query: 347 GGNGGAGGTGGVGVGGNLFTGGQGGAGGNAGLLA 380 G G G V + G + AG LA Sbjct: 71 SGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 31.6 bits (71), Expect = 0.008 Identities = 32/103 (31%), Positives = 39/103 (37%), Gaps = 7/103 (6%) Query: 347 GGNGGAGGTGGVGVGGNLFTGGQGGAGGNAGLLAGNG-GAGGTGGDAGMFGNGGAGGAGG 405 G N GA T G GG G GGA +G + N GG+G G G G GG Sbjct: 8 GHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGG 67 Query: 406 DRVAGSQGNGGDGGDGGHGGTYFGSGGAGGHGGYDDSGSGGQG 448 + GN G G G + + A G G+GG Sbjct: 68 N------GNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 30.1 bits (67), Expect = 0.026 Identities = 30/119 (25%), Positives = 41/119 (34%), Gaps = 10/119 (8%) Query: 408 VAGSQGNGGDGGDGGHGGTYFGSGGAGGHGGYDDSGSGGQGGHGGDGGAAGTIGNGGDGG 467 ++G G G + G G G G GG GSG + GG +G+ GG Sbjct: 1 MSGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGS--GIHWGG 58 Query: 468 TGGDALVSGGTGGDGGDGGDGGDAREIGNGGNGGNAGAGATAGNEGTGGTGGQLIGVSG 526 G G+GG G+ G G + A T G GG + +S Sbjct: 59 GSG--------HGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISA 109 Score = 30.1 bits (67), Expect = 0.028 Identities = 26/105 (24%), Positives = 34/105 (32%), Gaps = 4/105 (3%) Query: 129 TAPEQAGGDGSLLYGNGGAGGPGGAGGNAGLIGNGGAGGSGAALGLFGGSGAALGLFGGT 188 T G+ + G GG G N GGSG+ + GGSG G G Sbjct: 11 TGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNGN 70 Query: 189 GGNGGLLFGDGGTGGAAGDLASGVGLPGGAGGHAGLFGIGGAGGE 233 G G G +A G P + AG + + G Sbjct: 71 SGGG----SGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGA 111 Score = 29.7 bits (66), Expect = 0.033 Identities = 23/80 (28%), Positives = 25/80 (31%) Query: 298 SGADGGAGGNGGAGGGGLWFGNGGAGGIGGFDAHGDGGDGGAGGNAGIYGGNGGAGGTGG 357 SG DG G G G G+GG + G G G G GG G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 358 VGVGGNLFTGGQGGAGGNAG 377 G GG G G G Sbjct: 62 HGNGGGNGNSGGGSGTGGNL 81 Score = 29.7 bits (66), Expect = 0.033 Identities = 23/61 (37%), Positives = 27/61 (44%) Query: 219 GGHAGLFGIGGAGGEGGNSATTAGVGGAGGAGGLLVGAGGHGGTGGTSASGTGATGGSGG 278 GG GL GGA G S+ GG G+G G GHG GG SG G+ G Sbjct: 22 GGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNGNSGGGSGTGGNL 81 Query: 279 A 279 + Sbjct: 82 S 82

MUL_3284

MUL_3318

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
MUL_3284	2	15	-1.947584	prophage integrase
MUL_3285	4	18	3.496284	*GTP-binding protein EngA
MUL_3286	2	16	3.663924	cytidylate kinase
MUL_3287	2	14	4.133319	pseudouridylate synthase
MUL_3289	3	14	4.084423	transcriptional regulator
MUL_3291	2	16	4.587399	hypothetical protein
MUL_3292	1	17	5.925759	Soj family ATPase
MUL_3298	-1	11	2.543663	hypothetical protein
MUL_3299	-1	10	1.314403	N-term transposase for IS2404
MUL_3300	-1	11	1.055982	C-term transposase for IS2404
MUL_3301	0	12	0.157630	transposase for IS2606
MUL_3302	1	13	-0.455336	hypothetical protein
MUL_3303	2	12	-0.429817	transcriptional regulator
MUL_3304	2	13	-0.111393	hypothetical protein
MUL_3305	1	15	1.115997	PE-PGRS family protein
MUL_3307	1	14	0.911278	non-IS element not present in Mycobacterium
MUL_3308	-1	12	1.826258	non-IS element not present in Mycobacterium
MUL_3309	-1	10	2.200877	glutamine amidotransferase subunit PdxT
MUL_3310	-2	12	2.508175	acyl-CoA thioesterase II TesB2
MUL_3311	-2	10	1.657556	pyridoxal biosynthesis lyase PdxS
MUL_3312	2	17	3.629766	hypothetical protein
MUL_3313	1	14	3.120827	alpha-mannosyltransferase PimA
MUL_3314	1	14	3.078707	lipid A biosynthesis lauroyl acyltransferase
MUL_3315	1	16	2.294150	pi synthase PgsA1
MUL_3317	2	15	1.805530	hypothetical protein
MUL_3318	2	14	2.948849	threonyl-tRNA synthetase

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_3291	IGASERPTASE	30	0.018	IgA-specific serine endopeptidase (S6) signature.
>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature. Length = 1541 Score = 30.0 bits (67), Expect = 0.018 Identities = 17/51 (33%), Positives = 20/51 (39%), Gaps = 3/51 (5%) Query: 283 VTFDEDRHRAHTGNGAQVLATLRNTAINLHRLNGADNIAEACRITALTANR 333 V E+ H TGN L N I+ LN ADN + LT N Sbjct: 846 VRLTENSHWHLTGNSDVHQLDLANGHIH---LNSADNSNNVTKYNTLTVNS 893
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_3292	cloacin	39	6e-05	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 38.5 bits (89), Expect = 6e-05 Identities = 34/90 (37%), Positives = 39/90 (43%), Gaps = 5/90 (5%) Query: 498 GAGGQGGDGAQGGDGGAGVDTTTGAGGDGGAGAGGGTSGFLYGNGGAGGAGGHGGGGPGM 557 G G+G + G TG G GGA G G S GG G+G H GGG G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 558 GGDGGDGGDGGRAQLIGTGGNGGAGGTAAP 587 G GG+G GG G+G G AAP Sbjct: 63 GNGGGNGNSGG-----GSGTGGNLSAVAAP 87 Score = 35.5 bits (81), Expect = 6e-04 Identities = 37/127 (29%), Positives = 52/127 (40%), Gaps = 9/127 (7%) Query: 275 GGDAGAYGPPANGGAGG-HGGMGGVGGDAGLLFGSGGAGGVGGNGAIGGTGVMSGAGGAG 333 GGD + A+ +G +GG G+G G GSG + G G+G+ G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 334 GDGGNGGYAQLIGDGGGGGAGGAGYSASGLPPAGIGAPG-GAGGAGGSGGWLVGNGGAGG 392 G+GG G GGG+G G ++ P G P GAGG + + Sbjct: 63 GNGGGNG-------NSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAA 115 Query: 393 IGGIGAS 399 I I A+ Sbjct: 116 IADIMAA 122 Score = 34.3 bits (78), Expect = 0.002 Identities = 28/83 (33%), Positives = 34/83 (40%) Query: 159 SGGSAGLIGTGGAGGAGYGPAAQTGATGGSGGVGGTGGSGLDGPDSGAAGGAGGSGGSGG 218 SGG TG +G TG G G G+G S + P G +G GG G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 219 AARLFGNGGAGGSGGKGGNGGSA 241 GNG +GG G GGN + Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAV 84 Score = 33.5 bits (76), Expect = 0.002 Identities = 32/113 (28%), Positives = 35/113 (30%), Gaps = 6/113 (5%) Query: 348 GGGGGAGGAGYSASGLPPAGIGAPGGAGGAGGSGGWLVGNGGAGGIGGIGASGSFGAPSG 407 G G G +S SG G G GGA GW N GG G G G+ G Sbjct: 4 GDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHG 63 Query: 408 QGGNGGIGGAAGLFGQGGAGGNGGQGGGDDFALSGGAGGAGGAGGHGGQLYGD 460 GG G G G G A A GAGG + Sbjct: 64 NGGGNGNSGG------GSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAG 110 Score = 33.1 bits (75), Expect = 0.003 Identities = 27/84 (32%), Positives = 32/84 (38%), Gaps = 1/84 (1%) Query: 169 GGAGGAGYGPAAQTGATGGSGGVGGTGGSGLDGPDSGAAGGAGGSGGSGGAARLFGNGGA 228 G G G + +G G G GG DG + G GGSG G G Sbjct: 4 GDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWG-GGSGSGIHWGGGSGH 62 Query: 229 GGSGGKGGNGGSAIETGNDGAAGA 252 G GG G +GG + GN A A Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAA 86 Score = 32.8 bits (74), Expect = 0.004 Identities = 23/79 (29%), Positives = 27/79 (34%) Query: 486 GSGSVGGGAGLWGAGGQGGDGAQGGDGGAGVDTTTGAGGDGGAGAGGGTSGFLYGNGGAG 545 G G G G G G G G +G + GG SG +G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 546 GAGGHGGGGPGMGGDGGDG 564 G GG G G G GG+ Sbjct: 63 GNGGGNGNSGGGSGTGGNL 81 Score = 31.6 bits (71), Expect = 0.010 Identities = 31/101 (30%), Positives = 37/101 (36%), Gaps = 2/101 (1%) Query: 459 GDGGGGGAGGMGGSVDIAESGVTGGRGGSGSVGGGAGLWGAGGQGGDGAQGGDGGAGVDT 518 GDG G G S +I +G G G G G+G G G+ G G Sbjct: 4 GDGRGHNTGAHSTSGNI--NGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 519 TTGAGGDGGAGAGGGTSGFLYGNGGAGGAGGHGGGGPGMGG 559 GG+G +G G GT G L G PG GG Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG 102 Score = 31.2 bits (70), Expect = 0.012 Identities = 36/118 (30%), Positives = 47/118 (39%), Gaps = 15/118 (12%) Query: 186 GGSGGVGGTGGSGLDGPDSGAAGGAGGSGGSGGAARLFGNGGAGGSG-GKGGNGGSAIET 244 G + G T G+ ++G +G G G S GSG ++ GG GSG GG G Sbjct: 8 GHNTGAHSTSGN-INGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH---- 62 Query: 245 GNDGAAGAGGHGGWLVGNGGIGGDGGSGGVGGDAGAYGPPANGGAGGHGGMGGVGGDA 302 G GG G +GG G GG+ A+G PA G G + A Sbjct: 63 ------GNGGGNG---NSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGA 111 Score = 31.2 bits (70), Expect = 0.013 Identities = 26/80 (32%), Positives = 30/80 (37%), Gaps = 1/80 (1%) Query: 522 AGGDGGAGAGGGTSGFLYGNGGAGGAGGHGGGGPGMG-GDGGDGGDGGRAQLIGTGGNGG 580 +GGDG G S NGG G G GG G G + GG I GG G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 581 AGGTAAPGGTDGTAGAAGKG 600 G G + G +G G Sbjct: 62 HGNGGGNGNSGGGSGTGGNL 81 Score = 31.2 bits (70), Expect = 0.014 Identities = 29/87 (33%), Positives = 37/87 (42%), Gaps = 7/87 (8%) Query: 315 GGNGAIGGTGVMSGAGGAGGDGGNGGYAQLIGDGGGGGAGGAGYSASGLPPAGIGAPGGA 374 GG+G TG S +G G G GGG + G+G+S+ P G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLG-------VGGGASDGSGWSSENNPWGGGSGSGIH 55 Query: 375 GGAGGSGGWLVGNGGAGGIGGIGASGS 401 G G G GNG +GG G G + S Sbjct: 56 WGGGSGHGNGGGNGNSGGGSGTGGNLS 82 Score = 30.8 bits (69), Expect = 0.016 Identities = 31/103 (30%), Positives = 35/103 (33%), Gaps = 6/103 (5%) Query: 407 GQGGNGGIGGAAGLFGQGGAGGNGGQGGGDDFALSGGAGGAGGAGGHGGQLYGDGGGGGA 466 G+G N G +G G G G G D S GG G G G G G Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNG 65 Query: 467 GGMGGSVDIAESGVTGGRGGSGSVGGGAGLWGAGGQGGDGAQG 509 GG G SG G GG+ S +G GA G Sbjct: 66 GGNGN------SGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG 102 Score = 30.1 bits (67), Expect = 0.028 Identities = 30/87 (34%), Positives = 37/87 (42%), Gaps = 4/87 (4%) Query: 387 NGGAGGIGGIGASGSFGAPSGQGGNGGIGGAAGLFGQGGAGGNGGQGGGDDFALSGGAGG 446 +GG G GA + G +G G+GG A G G + N GGG + G G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGAS-DGSGWSSENNPWGGGSGSGIHWGGGS 60 Query: 447 AGGAGGHGGQLYGDGGGGGAGGMGGSV 473 G GG G GGG G GG +V Sbjct: 61 GHGNGGGNG---NSGGGSGTGGNLSAV 84 Score = 30.1 bits (67), Expect = 0.030 Identities = 32/98 (32%), Positives = 40/98 (40%), Gaps = 6/98 (6%) Query: 440 LSGGAGGAGGAGGHGGQLYGDGGGGGAGGMGGSVDIAESGVTGGRGGSGSVGGGAGLWGA 499 +SGG G G H +GG G G GG+ D + G GS G WG Sbjct: 1 MSGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGS--GSGIHWGG 58 Query: 500 GGQGGDGAQGGDGGAGVDTTTGAGGDGGAGAGGGTSGF 537 G G+G G+ G G +G GG+ A A GF Sbjct: 59 GSGHGNGGGNGNSGGG----SGTGGNLSAVAAPVAFGF 92 Score = 30.1 bits (67), Expect = 0.030 Identities = 31/114 (27%), Positives = 38/114 (33%), Gaps = 1/114 (0%) Query: 434 GGDDFALSGGAGGAGGAGGHGGQLYGDGGGGGAGGMGGSVDIAESGVTGGRGGSGSVGGG 493 GGD + GA G +GG GGG + G G S + G G G G G Sbjct: 3 GGDGRGHNTGAHSTSG-NINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 494 AGLWGAGGQGGDGAQGGDGGAGVDTTTGAGGDGGAGAGGGTSGFLYGNGGAGGA 547 G G G G G+ G + V G + G G G A Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAA 115 Score = 29.7 bits (66), Expect = 0.041 Identities = 33/106 (31%), Positives = 39/106 (36%), Gaps = 4/106 (3%) Query: 248 GAAGAGGHGGWLVGNGGIGGDGGSGGVGGDAGAYGPPANGGAGGHGGMGGVGGDAGLLFG 307 G G G + G +G I G GVGG A +G + + GG G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASD----GSGWSSENNPWGGGSGSGIHWGG 58 Query: 308 SGGAGGVGGNGAIGGTGVMSGAGGAGGDGGNGGYAQLIGDGGGGGA 353 G G GGNG GG G A G+ L G GG A Sbjct: 59 GSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_3303	SACTRNSFRASE	40	6e-07	Streptothricin acetyltransferase signature.
>SACTRNSFRASE#Streptothricin acetyltransferase signature. Length = 173 Score = 40.3 bits (94), Expect = 6e-07 Identities = 17/103 (16%), Positives = 31/103 (30%), Gaps = 2/103 (1%) Query: 33 DRFAEYLNDPQRTILAARAGGRIVGYAMLVRADTDDCDVELSKLYLLPDQHGTGTAKALM 92 D Y+ + + +G + +E + + D G AL+ Sbjct: 54 DMDVSYVEEEGKAAFLYYLENNCIGRIKIRSNWNGYALIE--DIAVAKDYRKKGVGTALL 111 Query: 93 DNALTTAQDWGARRVWLGVNQENQRAQRFCAKRGSTVSGTRTF 135 A+ A++ + L N A F AK + T Sbjct: 112 HKAIEWAKENHFCGLMLETQDINISACHFYAKHHFIIGAVDTM 154
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_3317	IGASERPTASE	30	0.016	IgA-specific serine endopeptidase (S6) signature.
>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature. Length = 1541 Score = 30.0 bits (67), Expect = 0.016 Identities = 17/51 (33%), Positives = 21/51 (41%), Gaps = 3/51 (5%) Query: 283 VTFDEDRHRAHTGNGAQVLATLRNTAINLHRLNGADNIADACRITALTANR 333 V E+ H TGN L N I+ LN ADN + + LT N Sbjct: 846 VRLTENSHWHLTGNSDVHQLDLANGHIH---LNSADNSNNVTKYNTLTVNS 893
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_3318	cloacin	38	1e-04	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 38.2 bits (88), Expect = 1e-04 Identities = 32/107 (29%), Positives = 41/107 (38%), Gaps = 5/107 (4%) Query: 301 GAGGTGGAGGTGGAGGDGLAATTAGGTGGNAGDGGEGGAGGNAGAGGAGGQGGLFGNAGT 360 G G G G G+ T G GG A DG + N GG+G G +G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 361 TGAGGDGGNGGNGGLAGNGGAGGNGDASNPNGGTGGNAANPGAGGAG 407 GG+G +GG G+G G + P + PGAGG Sbjct: 63 GNGGGNGNSGG-----GSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 36.2 bits (83), Expect = 4e-04 Identities = 31/102 (30%), Positives = 36/102 (35%) Query: 335 GEGGAGGNAGAGGAGGQGGLFGNAGTTGAGGDGGNGGNGGLAGNGGAGGNGDASNPNGGT 394 G G G N GA G G G G+G + GG G+G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 395 GGNAANPGAGGAGGAGGNGSRTGAPGTTGNTPTTAAGHGGKG 436 G N +GG G GGN S AP G + G GG Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 35.8 bits (82), Expect = 5e-04 Identities = 31/100 (31%), Positives = 38/100 (38%) Query: 408 GAGGNGSRTGAPGTTGNTPTTAAGHGGKGGDGFNPATSGQDGGSGGKGGDAGQFGNGGNG 467 G G G TGA T+GN G G GG S ++ GG G +G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 468 GNGAAGDAAGSGHTGGNGGAGGGGGNAGQFGEPGTGGSGG 507 GNG +G G G + A F T G+GG Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG 102 Score = 35.8 bits (82), Expect = 6e-04 Identities = 28/84 (33%), Positives = 34/84 (40%), Gaps = 4/84 (4%) Query: 462 GNGGNGGNGAAGDAAGSGHTGGNGGAGGGGGNAG----QFGEPGTGGSGGNGGKGGDGAA 517 G G G N A +G+ + G G GGG + G P GGSG GG Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 518 GGNGGTGGIGGTGGIGGDGRNGST 541 G GG G GG G GG+ + Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAA 86 Score = 33.9 bits (77), Expect = 0.002 Identities = 25/86 (29%), Positives = 32/86 (37%) Query: 243 GHGGDGGTGGTGASAAAGANGGAGQTGLAGTDGGTGGASGEGGAGGKGGLLFGAGGEGGA 302 G G + G T + G G G + G + + GG G G G G G Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNG 65 Query: 303 GGTGGAGGTGGAGGDGLAATTAGGTG 328 GG G +GG G GG+ A G Sbjct: 66 GGNGNSGGGSGTGGNLSAVAAPVAFG 91 Score = 33.5 bits (76), Expect = 0.002 Identities = 22/75 (29%), Positives = 31/75 (41%) Query: 449 GGSGGKGGDAGQFGNGGNGGNGAAGDAAGSGHTGGNGGAGGGGGNAGQFGEPGTGGSGGN 508 G + G +G G G G + GSG + N GGG G+ +G G+GG Sbjct: 8 GHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGG 67 Query: 509 GGKGGDGAAGGNGGT 523 G G G+ G + Sbjct: 68 NGNSGGGSGTGGNLS 82 Score = 33.1 bits (75), Expect = 0.004 Identities = 25/80 (31%), Positives = 33/80 (41%) Query: 481 TGGNGGAGGGGGNAGQFGEPGTGGSGGNGGKGGDGAAGGNGGTGGIGGTGGIGGDGRNGS 540 +GG+G G ++ G G GG DG+ + GG+G G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 541 TGAIGGNGGTGGTGGTGGTG 560 G GGNG +GG GTGG Sbjct: 62 HGNGGGNGNSGGGSGTGGNL 81 Score = 32.8 bits (74), Expect = 0.005 Identities = 29/80 (36%), Positives = 37/80 (46%) Query: 505 SGGNGGKGGDGAAGGNGGTGGIGGTGGIGGDGRNGSTGAIGGNGGTGGTGGTGGTGGKSL 564 SGG+G GA +G G G+GG +GS + N GG+G GG S Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 565 GNLPSGDGGVGGNAGTGGNG 584 G+G GG +GTGGN Sbjct: 62 HGNGGGNGNSGGGSGTGGNL 81 Score = 32.4 bits (73), Expect = 0.007 Identities = 36/102 (35%), Positives = 41/102 (40%), Gaps = 9/102 (8%) Query: 533 GGDGRNGSTGAI-------GGNGGTGGTGGTGGTGGKSLGNLPSGDGGVGGNAGTGGNGG 585 GGDGR +TGA GG G G GG G S N P G GG G GG G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWG-GGSGSGIHWGGGSG 61 Query: 586 NATDGGAGGKGGDGAAGGTGGNAGEFQSLLGISKGGDGGTGG 627 + +GG G G G+ G +A G G GG Sbjct: 62 HG-NGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG 102 Score = 31.6 bits (71), Expect = 0.010 Identities = 42/131 (32%), Positives = 50/131 (38%), Gaps = 12/131 (9%) Query: 175 GGAGGAGGAAGLIGSGGAGGIGGTGAAGGKGGNAVLFGAGGNGGIGGTGAVGGTGAVGGA 234 G GA +G I G G G GA+ G G ++ GG G G G GG Sbjct: 8 GHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGG 67 Query: 235 GGNGGFLFGHGGDGGTGGTGASAAAGANG-------GAGQTGLAGTDGGTGGASGEGGAG 287 GN G GG G G A AA A G GAG ++ + G A + A Sbjct: 68 NGNSG-----GGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAAIADIMAA 122 Query: 288 GKGGLLFGAGG 298 KG FG G Sbjct: 123 LKGPFKFGLWG 133 Score = 30.1 bits (67), Expect = 0.036 Identities = 23/80 (28%), Positives = 30/80 (37%) Query: 430 AGHGGKGGDGFNPATSGQDGGSGGKGGDAGQFGNGGNGGNGAAGDAAGSGHTGGNGGAGG 489 +G G+G + +TSG G G G +G + GSG GG G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 490 GGGNAGQFGEPGTGGSGGNG 509 G G G G+GGN Sbjct: 62 HGNGGGNGNSGGGSGTGGNL 81 Score = 30.1 bits (67), Expect = 0.038 Identities = 21/82 (25%), Positives = 26/82 (31%) Query: 270 LAGTDGGTGGASGEGGAGGKGGLLFGAGGEGGAGGTGGAGGTGGAGGDGLAATTAGGTGG 329 ++G DG +G G G G GGA G G G + G G Sbjct: 1 MSGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGS 60 Query: 330 NAGDGGEGGAGGNAGAGGAGGQ 351 G+GG G G G Sbjct: 61 GHGNGGGNGNSGGGSGTGGNLS 82

MUL_3480

MUL_3507

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
MUL_3480	-2	17	-3.171900	transcriptional regulator
MUL_3481	-1	15	-2.280247	acyl-CoA dehydrogenase FadE22
MUL_3483	-2	15	-1.682677	short-chain type dehydrogenase/reductase
MUL_3484	-2	16	-1.918623	ATP-dependent DNA ligase
MUL_3486	-2	17	-1.591623	hypothetical protein
MUL_3487	0	16	-1.236073	transcriptional regulatory protein
MUL_3490	2	15	-0.370388	hypothetical protein
MUL_3491	1	15	-1.204287	transposase for IS2606
MUL_3492	0	14	-0.492246	transposase for IS2404
MUL_3493	1	13	-0.251880	chaperone protein DnaK1
MUL_3494	2	14	0.176358	short chain dehydrogenase
MUL_3495	1	12	1.032033	hypothetical protein
MUL_3496	1	12	1.794643	multidrug-transport integral membrane protein
MUL_3497	-1	12	1.983284	transcriptional regulatory protein
MUL_3498	-1	11	2.044807	hypothetical protein
MUL_3499	-2	13	1.903031	hypothetical protein
MUL_3500	-1	12	2.222969	hydrolase
MUL_3501	0	13	2.434199	succinate-semialdehyde dehydrogenase [NADP+]
MUL_3502	2	13	2.917161	fatty-acid-CoA ligase
MUL_3503	1	11	2.795218	hypothetical protein
MUL_3504	1	13	3.117599	hypothetical protein
MUL_3505	1	12	3.488348	oxidoreductase GMC-type
MUL_3506	2	12	3.055198	transposase for IS2404
MUL_3507	3	12	2.750768	transposase for IS2606

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_3481	IGASERPTASE	27	0.022	IgA-specific serine endopeptidase (S6) signature.
>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature. Length = 1541 Score = 27.0 bits (59), Expect = 0.022 Identities = 16/51 (31%), Positives = 19/51 (37%), Gaps = 3/51 (5%) Query: 49 VTFDEDRQRAHTGNGAQVLATLRNTAINLHRLNGADNIAEACRITALTANR 99 V E+ TGN L N I+ LN ADN + LT N Sbjct: 846 VRLTENSHWHLTGNSDVHQLDLANGHIH---LNSADNSNNVTKYNTLTVNS 893
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_3486	IGASERPTASE	30	0.018	IgA-specific serine endopeptidase (S6) signature.
>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature. Length = 1541 Score = 30.0 bits (67), Expect = 0.018 Identities = 17/51 (33%), Positives = 20/51 (39%), Gaps = 3/51 (5%) Query: 283 VTFDEDRHRAHTGNGAQVLATLRNTAINLHRLNGADNIAEACRITALTANR 333 V E+ H TGN L N I+ LN ADN + LT N Sbjct: 846 VRLTENSHWHLTGNSDVHQLDLANGHIH---LNSADNSNNVTKYNTLTVNS 893
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_3493	IGASERPTASE	30	0.007	IgA-specific serine endopeptidase (S6) signature.
>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature. Length = 1541 Score = 30.4 bits (68), Expect = 0.007 Identities = 29/180 (16%), Positives = 59/180 (32%), Gaps = 19/180 (10%) Query: 36 ENELTRLIEENSDLRQRIAELDQELAAGAGGGAAVTAQPTQAMPVYEPEPEPAKPAAPVA 95 N L + R + + + A V + P+ + + P P AP Sbjct: 974 VNGRYDLYNPEVEKRNQTVDTTN-ITTPNNIQADVPSVPSNNEEIARVDEAPVPPPAPAT 1032 Query: 96 SAATNEEQAMKAARVLSLAQDTADRLTSTAKAESDKMLSDARANADQILSEARHT--AET 153 + T E A + + S ++++ ++ A ++ EA+ A T Sbjct: 1033 PSETTETVAENSKQE------------SKTVEKNEQDATETTAQNREVAKEAKSNVKANT 1080 Query: 154 TVTE-ARQRADGMLADAQARSESQLRQAQEKADAL---QADAERKHSEIMGTINQQRTVL 209 E A+ ++ E+ + +EKA + + S++ Q TV Sbjct: 1081 QTNEVAQSGSETKETQTTETKETATVEKEEKAKVETEKTQEVPKVTSQVSPKQEQSETVQ 1140
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_3502	PF03544	33	0.001	Gram-negative bacterial tonB protein
>PF03544#Gram-negative bacterial tonB protein Length = 243 Score = 33.4 bits (76), Expect = 0.001 Identities = 27/149 (18%), Positives = 51/149 (34%), Gaps = 7/149 (4%) Query: 387 FINIGYVIGLLPVTGLQLPLISAGGTSTATTLAMIGIIANAARHEPEAVAALRAGRDDRV 446 I+ V GLL + Q+ + A + T+ +A A P+AV + Sbjct: 23 CIHGAVVAGLLYTSVHQVIELPAPAQPISVTM-----VAPADLEPPQAVQPPPEPVVEPE 77 Query: 447 NRMLRLPLPKPYAPTRLEVFRDRKRVQPPAARPPAKQAAARKAPKAATRLAEEPLRPALP 506 +P P AP +E + + + +P + + K ++ E PA P Sbjct: 78 PEPEPIPEPPKEAPVVIEKPKPKPKPKPKPVKKVEQPKRDVKPVESRPASPFENTAPARP 137 Query: 507 RRPDRSGARSGQQGAGQRYAGQRHSGRVR 535 + + + +G R R + Sbjct: 138 --TSSTATAATSKPVTSVASGPRALSRNQ 164
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_3507	cloacin	41	1e-05	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 40.9 bits (95), Expect = 1e-05 Identities = 39/119 (32%), Positives = 49/119 (41%), Gaps = 17/119 (14%) Query: 408 SGGSGGIGGTGGHSLINSGGGIGGKGGGGGAAGLIGDGGAGGAGGNGGDGTGAGGLGGNG 467 SGG G TG HS + G I G G G G G + G+G GG+G Sbjct: 2 SGGDGRGHNTGAHS---TSGNINGGPTGLG-------VGGGASDGSGWSSENNPWGGGSG 51 Query: 468 ASADWIGNGGSGGAGGSGGLGAHAGAGGNGGSLYGNDGSG-------GAGGTGASGAGG 519 + W G G G GG+G G +G GGN ++ G GAGG S + G Sbjct: 52 SGIHWGGGSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAG 110 Score = 37.4 bits (86), Expect = 2e-04 Identities = 30/78 (38%), Positives = 31/78 (39%), Gaps = 1/78 (1%) Query: 160 GGNGGAAGLIGNGGAGGSGWAGGAGGAGGNGGWLYGNGGAGGLGGAAAGDYTAGGVGGAG 219 G N GA GN GG G GGA GW N GG G+ G G G Sbjct: 8 GHNTGAHSTSGNIN-GGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGG 66 Query: 220 GNAGLWGDGGAGGNGSAT 237 GN G G GGN SA Sbjct: 67 GNGNSGGGSGTGGNLSAV 84 Score = 37.0 bits (85), Expect = 2e-04 Identities = 29/82 (35%), Positives = 37/82 (45%), Gaps = 1/82 (1%) Query: 446 GAGGAGGNGGDGTGAGGLGGNGASADWIGNGGSGGAGGSGGLGAHAGAGGNGGSLYGNDG 505 G G G N G + +G + G G + +G G S G+G S G G+G G G Sbjct: 3 GGDGRGHNTGAHSTSGNING-GPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 506 SGGAGGTGASGAGGNGGGGGAA 527 G GG G SG G GG +A Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSA 83 Score = 35.8 bits (82), Expect = 4e-04 Identities = 27/78 (34%), Positives = 35/78 (44%), Gaps = 6/78 (7%) Query: 483 GSGGLGAHAGAGGNGGSLYGNDGSGGAGGTGASGAG------GNGGGGGAAGMMGDGGAG 536 G G G + GA G++ G G GG + G+G GGG G+ G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 537 GDGGDGSAAGGLGGDGGN 554 G+GG +GG G GGN Sbjct: 63 GNGGGNGNSGGGSGTGGN 80 Score = 35.5 bits (81), Expect = 6e-04 Identities = 29/88 (32%), Positives = 37/88 (42%), Gaps = 4/88 (4%) Query: 458 TGAGGLGGNGASADWIGNGGSGGAGGSGGLGAHAGAGGNGGSLYGNDGSGGAGGTGASGA 517 +G G G N + GN G G G GA G+G + + GSG G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 518 GGNGGGGGAAGMMGDGGAGGDGGDGSAA 545 GNGGG G +G GG+G G + A Sbjct: 62 HGNGGGNGNSG----GGSGTGGNLSAVA 85 Score = 34.7 bits (79), Expect = 0.001 Identities = 33/98 (33%), Positives = 41/98 (41%), Gaps = 5/98 (5%) Query: 125 GAAGTAASPNGGAGGLLYGNGGAGYSYTSGATAEAGGNGGAAGLIGNGGAGGSGWAGGAG 184 GA T+ + NGG GL G G S SG ++E GG +G GG G G Sbjct: 12 GAHSTSGNINGGPTGL---GVGGGASDGSGWSSENNPWGGGSG--SGIHWGGGSGHGNGG 66 Query: 185 GAGGNGGWLYGNGGAGGLGGAAAGDYTAGGVGGAGGNA 222 G G +GG G + A + A GAGG A Sbjct: 67 GNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 32.8 bits (74), Expect = 0.004 Identities = 33/86 (38%), Positives = 36/86 (41%), Gaps = 6/86 (6%) Query: 369 SGLIGDGGAGGAGGTGGGNAGVGGVGGVGGTGGAARLFGSGGSGGIGGTGGHSLINSGGG 428 SG G G GA T G G G G+G GGA+ G G G S I+ GGG Sbjct: 2 SGGDGRGHNTGAHSTSGNING--GPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGG 59 Query: 429 IGGKGGGGGAAGLIGDGGAGGAGGNG 454 G GGG GG G GGN Sbjct: 60 SGHGNGGGNG----NSGGGSGTGGNL 81 Score = 32.4 bits (73), Expect = 0.005 Identities = 27/89 (30%), Positives = 31/89 (34%) Query: 319 GDGGTGGNGGLLSGDGGAGGVGGTGGIQIYSGGGIGGVGGIGGNGGAGGTSGLIGDGGAG 378 G G G N G S G G G+ + G G GG G+ G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 379 GAGGTGGGNAGVGGVGGVGGTGGAARLFG 407 G GG G + G G GG A FG Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFG 91 Score = 32.4 bits (73), Expect = 0.006 Identities = 32/85 (37%), Positives = 35/85 (41%), Gaps = 8/85 (9%) Query: 335 GAGGVGGTGGIQIYSGGGIGGVGGIGGNGGAGGTSGLIGDGGAGGAGGTGGGNAGVGGVG 394 G G G G SG GG G+G GGA SG + G G G GG Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGS--GSGIHWGGGS 60 Query: 395 GVGGTGGAARLFGSGGSGGIGGTGG 419 G G GG +G SGG GTGG Sbjct: 61 GHGNGGG------NGNSGGGSGTGG 79 Score = 32.4 bits (73), Expect = 0.006 Identities = 31/97 (31%), Positives = 36/97 (37%), Gaps = 6/97 (6%) Query: 502 GNDGSGGAGGTGASGAGGNGGGGGAAGMMGDGGAGGDGGDGSAAGGL--GGDGGNADWLG 559 G DG G G ++ NGG G +G GG DG S+ GG G W G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTG----LGVGGGASDGSGWSSENNPWGGGSGSGIHWGG 58 Query: 560 NGGGGTGGFGLPPATGGFGGRGGRLFGSPGAQGRRAL 596 G G GG G G +P A G AL Sbjct: 59 GSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPAL 95 Score = 30.5 bits (68), Expect = 0.021 Identities = 26/77 (33%), Positives = 31/77 (40%) Query: 294 GGNAGLWGNGGAGGDGGFGGTATVIGDGGTGGNGGLLSGDGGAGGVGGTGGIQIYSGGGI 353 GG+ G G G T +G GG +G S + G G GI G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 354 GGVGGIGGNGGAGGTSG 370 G GG G +GG GT G Sbjct: 63 GNGGGNGNSGGGSGTGG 79

MUL_3667

MUL_3676

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
MUL_3667	0	16	3.488197	putative regulatory protein
MUL_3668	2	15	4.825151	anti sigma factor antagonist
MUL_3669	1	13	4.009987	hypothetical protein
MUL_3670	0	11	3.260949	transposase for IS2606
MUL_3671	1	13	3.111772	transposase for IS2606
MUL_3672	0	13	2.205434	glycyl-tRNA synthetase
MUL_3673	0	10	-0.939142	ArsR family transcriptional regulator
MUL_3674	0	10	0.374774	ferric uptake regulation protein FurB
MUL_3675	1	9	1.257531	hypothetical protein
MUL_3676	2	7	1.217868	undecaprenyl pyrophosphate synthase

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_3667	TCRTETOQM	89	1e-20	Tetracycline resistance protein TetO/TetQ/TetM family ...
>TCRTETOQM#Tetracycline resistance protein TetO/TetQ/TetM family signature. Length = 639 Score = 89.1 bits (221), Expect = 1e-20 Identities = 45/119 (37%), Positives = 67/119 (56%), Gaps = 9/119 (7%) Query: 6 GVVDERSMRAQYLDRMDIERERGITIKAQNVRLPWQLDGTEYVLHLIDTPGHVDFTYEVS 65 G VD+ + R D +ER+RGITI+ W + T+ +++IDTPGH+DF EV Sbjct: 34 GSVDKGTTRT---DNTLLERQRGITIQTGITSFQW--ENTK--VNIIDTPGHMDFLAEVY 86 Query: 66 RALEACEGAVLLVDAAQGIEAQTLANLYLALDR-DLHIIPVLNKIDLPAADPDRYAGEI 123 R+L +GA+LL+ A G++AQT L+ AL + + I +NKID D +I Sbjct: 87 RSLSVLDGAILLISAKDGVQAQTRI-LFHALRKMGIPTIFFINKIDQNGIDLSTVYQDI 144 Score = 82.2 bits (203), Expect = 2e-18 Identities = 43/234 (18%), Positives = 84/234 (35%), Gaps = 18/234 (7%) Query: 120 AGEIAHIIGCEPGDVLRVSGKTGEGVADLLDHVVREVPPPQGDADAPTRAMIFDSVYDIY 179 E C V S K G+ +L++ + + + +F Y Sbjct: 202 QEESIRFHNCSLFPVYHGSAKNNIGIDNLIEVITNKFYSSTHRGQSELCGKVFKIEYSEK 261 Query: 180 RGVVTYVRVVDGKITPRERIAMMSTGATHELLEVGIVSPEPKASDGLGVGEVGYL---IT 236 R + Y+R+ G + R+ + + ++ E D GE+ L Sbjct: 262 RQRLAYIRLYSGVLHLRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFL 321 Query: 237 GVKDVWQSKVGDTVTTARKGATEALTGYREPKPMVYSGLYPVDGSDYPNLRDALDKLQLN 296 + V +GDT ++ E P P++ + + P L DAL ++ + Sbjct: 322 KLNSV----LGDTKLLPQRERIEN------PLPLLQTTVEPSKPQQREMLLDALLEISDS 371 Query: 297 DAALTYE-PETSVALGFGFRCGFLGLLHMEISRERLEREFDLDLISTSPNVVYR 349 D L Y + + FLG + ME++ L+ ++ +++ P V+Y Sbjct: 372 DPLLRYYVDSATHEIIL----SFLGKVQMEVTCALLQEKYHVEIEIKEPTVIYM 421 Score = 32.5 bits (74), Expect = 0.004 Identities = 16/81 (19%), Positives = 26/81 (32%), Gaps = 2/81 (2%) Query: 374 VYEPVVKTTIIAPSEFIGTIMELCQSRRGELGGMDYLSPERVELRYTMPLGEIIFDFFDS 433 + EP + I AP E++ + + E V L +P I ++ Sbjct: 535 LLEPYLSFKIYAPQEYLSRAYTDAPKYCANIVDTQLKNNE-VILSGEIPARC-IQEYRSD 592 Query: 434 LISRTRGYASLDYEESGEQEA 454 L T G + E G Sbjct: 593 LTFFTNGRSVCLTELKGYHVT 613
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_3668	PHPHLIPASEA1	28	0.028	Bacterial phospholipase A1 protein signature.
>PHPHLIPASEA1#Bacterial phospholipase A1 protein signature. Length = 289 Score = 27.6 bits (61), Expect = 0.028 Identities = 22/96 (22%), Positives = 36/96 (37%), Gaps = 14/96 (14%) Query: 56 PTAARARKLTYSPDHDGRADPGEIVWTWVVYEDDPTQGQDRPVLVVGRERNVLLALMLSS 115 P A A++ T HD A G I+ + Q D P + + N L+ Sbjct: 15 PMAVYAQEATVKEVHDAPAVRGSIIANML-------QEHDNPFTLYPYDTNYLIYT---- 63 Query: 116 QEQYSADPDWVAIGTGDWDFEGQQGWVRLDRVLDVP 151 ++D + AI + DW ++ V+ L P Sbjct: 64 ---QTSDLNKEAIASYDWAENARKDEVKFQLSLAFP 96
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_3671	cloacin	39	4e-05	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 38.9 bits (90), Expect = 4e-05 Identities = 34/90 (37%), Positives = 39/90 (43%), Gaps = 5/90 (5%) Query: 290 GDGGNGGAGGGQG-ANGGLGGLVIGNGGNRGIGGAGDSGNPAGGQGGNGGGAYLIGNGGW 348 G G N GA G NGG GL +G G + G G NP GG G+G G G Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGGASDG-SGWSSENNPWGGGSGSGIHW---GGGSG 61 Query: 349 GGVGGSGGDGGWLLGNGGNGAEGGTSSATG 378 G GG G+ G G GGN + A G Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAAPVAFG 91 Score = 34.7 bits (79), Expect = 6e-04 Identities = 31/105 (29%), Positives = 42/105 (40%), Gaps = 2/105 (1%) Query: 140 GNGGNGGDSTSAGVAGGAGGSAGLFGNGGAGGTGADADVSATNGGAGGAGGNAGLIFGFG 199 G G N G +++G G G GL GGA + + GG G+G + G G G Sbjct: 6 GRGHNTGAHSTSGNING--GPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHG 63 Query: 200 GAGGTGGSGINSFASFGGDGGAGGNSYLLGAAGAGGNGGVGLGTS 244 GG G SG S A ++ A G GG+ + S Sbjct: 64 NGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSIS 108 Score = 33.9 bits (77), Expect = 0.001 Identities = 27/92 (29%), Positives = 33/92 (35%), Gaps = 11/92 (11%) Query: 249 TGGDGGQGGLFGNGAAGGLGGQSTDGDGGSGGSGGRAGILYGDGGNGGAGGGQGANGGLG 308 +GGDG + +G + G T G G S G G GG Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDG-----------SGWSSENNPWGGGS 50 Query: 309 GLVIGNGGNRGIGGAGDSGNPAGGQGGNGGGA 340 G I GG G G G +GN GG G G + Sbjct: 51 GSGIHWGGGSGHGNGGGNGNSGGGSGTGGNLS 82 Score = 32.4 bits (73), Expect = 0.004 Identities = 28/90 (31%), Positives = 38/90 (42%), Gaps = 6/90 (6%) Query: 332 GQGGNGGGAYLIGN--GGWGGVGGSGGDGGWLLGNGGNGAEGGTSSATGGDGGNGGDARF 389 G+G N G GN GG G+G GG + N GG S + GG G Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH--- 62 Query: 390 IGNGGDGAHGGDGTPDGASGTGGSGGILFG 419 GNGG + G G+ G + + + + FG Sbjct: 63 -GNGGGNGNSGGGSGTGGNLSAVAAPVAFG 91 Score = 32.0 bits (72), Expect = 0.005 Identities = 37/129 (28%), Positives = 43/129 (33%), Gaps = 17/129 (13%) Query: 290 GDGGNGGAGGGQGANGGLGGLVIGNGGNRGIGGAGDSGNPAGGQGGNGGGAYLIGNGGWG 349 G G G G +G + NGG G+G G G + G + N WG Sbjct: 3 GGDGRGHNTGAHSTSGNI------NGGPTGLGVGG---------GASDGSGWSSENNPWG 47 Query: 350 GVGGSGGD--GGWLLGNGGNGAEGGTSSATGGDGGNGGDARFIGNGGDGAHGGDGTPDGA 407 G GSG GG GNGG G S TGG+ G G G Sbjct: 48 GGSGSGIHWGGGSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSI 107 Query: 408 SGTGGSGGI 416 S S I Sbjct: 108 SAGALSAAI 116 Score = 30.8 bits (69), Expect = 0.012 Identities = 25/79 (31%), Positives = 29/79 (36%) Query: 229 GAAGAGGNGGVGLGTSRFGGTGGDGGQGGLFGNGAAGGLGGQSTDGDGGSGGSGGRAGIL 288 G G G N G + G G GG +G+ G GSG G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 289 YGDGGNGGAGGGQGANGGL 307 GGNG +GGG G G L Sbjct: 63 GNGGGNGNSGGGSGTGGNL 81 Score = 30.5 bits (68), Expect = 0.015 Identities = 24/92 (26%), Positives = 34/92 (36%), Gaps = 1/92 (1%) Query: 119 GADGQTVNGVGQAGGDGGFLWGNGGNGGDSTSAGVAGGAGGSAGLFGNGGAGGTGADADV 178 GA + N G G G + G+G S + GG+G G G G G + + Sbjct: 12 GAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNGNS 71 Query: 179 SATNGGAGGAGGNAG-LIFGFGGAGGTGGSGI 209 +G G A + FGF G G+ Sbjct: 72 GGGSGTGGNLSAVAAPVAFGFPALSTPGAGGL 103 Score = 30.5 bits (68), Expect = 0.016 Identities = 39/117 (33%), Positives = 47/117 (40%), Gaps = 14/117 (11%) Query: 165 GNGGAGGTGADADVSATNGGAGGAGGNAGLIFGFGGAGGTGGSGINSFASFGGDGGAGGN 224 G+G TGA + NGG G G G G + GSG +S + G GG+G Sbjct: 4 GDGRGHNTGAHSTSGNINGGPTGLGV---------GGGASDGSGWSSENNPWG-GGSGSG 53 Query: 225 SYLLGAAGAGGNGGVGLGTSRFGGTGGDGGQGGLFGNGAAGGLGGQSTDGDGGSGGS 281 + G +G G GG G GG G GG A G ST G GG S Sbjct: 54 IHWGGGSGHGNGGGNGNS----GGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVS 106
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_3672	cloacin	38	8e-05	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 37.8 bits (87), Expect = 8e-05 Identities = 30/84 (35%), Positives = 34/84 (40%) Query: 155 GGGGGYAGLIGNGGAGGTGGNGGSGGLGGNAWLLGSGGTGGTGGAGVSSGFGGTGGNGGL 214 G G GN G TG G G G+ W + GG G+G+ G G GNGG Sbjct: 8 GHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGG 67 Query: 215 GGLLYGSGGAGGTGGAGAAGAVLG 238 G G G GG A AA G Sbjct: 68 NGNSGGGSGTGGNLSAVAAPVAFG 91 Score = 37.4 bits (86), Expect = 1e-04 Identities = 36/111 (32%), Positives = 43/111 (38%), Gaps = 2/111 (1%) Query: 265 GLTGQGGQGGDAGAGGASGYGIGNGGAGGAAGDGGGAGSLGTGGNGGSGGRAALLYGVGR 324 G G+G G G G G GG A DG G S GGSG + + +G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSG--SGIHWGGGS 60 Query: 325 DGGAGGAGGDGVGSAGGSGGLGGAAGLVDVGGDGGAGGAGGGAGGDGGAGA 375 G GG G+ G +G G L A V G + GG AGA Sbjct: 61 GHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGA 111 Score = 35.1 bits (80), Expect = 5e-04 Identities = 36/103 (34%), Positives = 43/103 (41%), Gaps = 1/103 (0%) Query: 244 GGDARLFGTGGAGGTGGDNSLGLTGQGGQGGDAGAGGASGYGIGNGGAGGAAGDGGGAGS 303 GGD R TG +G N G G G+G +S GG+G GGG+G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 304 LGTGGNGGSGGRAALLYGVGRDGGAGGAGGDGVGSAGGSGGLG 346 GGNG SGG + G A A G S G+GGL Sbjct: 63 GNGGGNGNSGGGSG-TGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 35.1 bits (80), Expect = 5e-04 Identities = 39/122 (31%), Positives = 45/122 (36%), Gaps = 7/122 (5%) Query: 118 NGADGATVGRI-GTPGGAGGLLYGNGGRGGDSTLAGVSGGGGGYAGLIGNGGAGGTGGNG 176 N +T G I G P G G + G G S GG G G G G GGNG Sbjct: 10 NTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNG 69 Query: 177 GSGGLGGNAWLLGSGGTGGTGGAGVSSGFGGTGGNGGLGGLLYGSGGAGGTGGAGAAGAV 236 SGG G+GG A V+ GF G G + S GA A A+ Sbjct: 70 NSGGGS------GTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAAIADIMAAL 123 Query: 237 LG 238 G Sbjct: 124 KG 125 Score = 34.3 bits (78), Expect = 0.001 Identities = 25/76 (32%), Positives = 33/76 (43%) Query: 321 GVGRDGGAGGAGGDGVGSAGGSGGLGGAAGLVDVGGDGGAGGAGGGAGGDGGAGAYLIGD 380 G G + GA G+ G G G GGA+ + G G G+G G G+ Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNG 65 Query: 381 GGDGGAGGHGGDGGAG 396 GG+G +GG G GG Sbjct: 66 GGNGNSGGGSGTGGNL 81 Score = 33.9 bits (77), Expect = 0.001 Identities = 36/124 (29%), Positives = 42/124 (33%), Gaps = 17/124 (13%) Query: 220 GSGGAGGTGGAGAAGAVLGGMGGLGGDARLFGTGGAGGTGGDNSLGLTGQGGQGGDAGAG 279 G G TG +G + GG GLG G G + G Sbjct: 4 GDGRGHNTGAHSTSGNINGGPTGLGV----------------GGGASDGSGWSSENNPWG 47 Query: 280 GASGYGIGNGGAGGAAGDGGGAGSLGTGGNGGSGGRAALLYGVGRDG-GAGGAGGDGVGS 338 G SG GI GG G GG S G G GG+ A G GAGG V Sbjct: 48 GGSGSGIHWGGGSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSI 107 Query: 339 AGGS 342 + G+ Sbjct: 108 SAGA 111 Score = 32.0 bits (72), Expect = 0.005 Identities = 28/82 (34%), Positives = 35/82 (42%), Gaps = 1/82 (1%) Query: 365 GGAGGDGGAGAYLIGDGGDGGAGGHGGDGGAG-GDGNAGSLAGGTGGNGGDAKVIGNGGN 423 GG G GA+ +GG G G GGA G G + GG+G G G+ Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 424 GGDGGVRFGGGANGSGGTGGAA 445 G GG GG +G+GG A Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAV 84 Score = 29.3 bits (65), Expect = 0.033 Identities = 25/80 (31%), Positives = 29/80 (36%), Gaps = 1/80 (1%) Query: 133 GAGGLLYGNGGRGGDSTLAGVSGGGGGYAGLIGNGGAGGTGGNGGSGGLGGNAWLLGSGG 192 G G + G + G G G G +G + N GG G G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGG-ASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 193 TGGTGGAGVSSGFGGTGGNG 212 G GG G S G GTGGN Sbjct: 62 HGNGGGNGNSGGGSGTGGNL 81
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_3675	56KDTSANTIGN	32	0.007	Rickettsia 56kDa type-specific antigen protein sign...
>56KDTSANTIGN#Rickettsia 56kDa type-specific antigen protein signature. Length = 533 Score = 31.9 bits (72), Expect = 0.007 Identities = 12/21 (57%), Positives = 13/21 (61%) Query: 533 NQQQPQQNQPQEGQHQQQQQQ 553 N P Q Q Q+GQ QQQQ Q Sbjct: 333 NFVMPPQAQQQQGQGQQQQAQ 353

MUL_3708

MUL_3715

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
MUL_3708	3	11	0.572540	putative transcriptional regulator
MUL_3711	4	11	0.759920	acyl-[acyl-carrier protein] desaturase DesA1
MUL_3712	4	13	0.704986	hypothetical protein
MUL_3713	4	12	0.241407	salicylate synthase MbtI
MUL_3714	2	10	-0.288832	hypothetical protein
MUL_3715	2	10	0.049075	membrane permease

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_3708	HTHTETR	55	2e-11	TetR bacterial regulatory protein HTH signature.
>HTHTETR#TetR bacterial regulatory protein HTH signature. Length = 215 Score = 54.6 bits (131), Expect = 2e-11 Identities = 26/167 (15%), Positives = 57/167 (34%), Gaps = 11/167 (6%) Query: 6 RNAQANRRQRREQMECRLLEATERLMNNGASFTELSVDRLATEAGISRASFYIYFDDKGH 65 R + ++ R+ +L+ RL + + S+ +A AG++R + Y +F DK Sbjct: 3 RKTKQEAQETRQ----HILDVALRLFSQQ-GVSSTSLGEIAKAAGVTRGAIYWHFKDKSD 57 Query: 66 LLRRLAGQVFDDLATGAQHWWDVAWRHDPDDVRAAMCAII------ARYRRHQPILIALN 119 L + ++ + +R + ++ R R I+ Sbjct: 58 LFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVTEERRRLLMEIIFHKC 117 Query: 120 EMAGYEPQTAQTYRDILTAISARLARVIEDGQADGSIRPELSATTTA 166 E G Q R++ R+ + ++ + +L A Sbjct: 118 EFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMTRRAA 164
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_3711	CARBMTKINASE	37	9e-05	Bacterial carbamate kinase signature.
>CARBMTKINASE#Bacterial carbamate kinase signature. Length = 314 Score = 36.7 bits (85), Expect = 9e-05 Identities = 24/104 (23%), Positives = 43/104 (41%), Gaps = 7/104 (6%) Query: 156 DNDRLSALVAHLVGADALVLLSDIDGLYDADPRKFQNARFIPEVSGPADLDGVVAGQGSH 215 D D +A V AD ++L+D++G + +++ EV +L + H Sbjct: 214 DKDLAGEKLAEEVNADIFMILTDVNGAA-LYYGT-EKEQWLREVK-VEELRKYY--EEGH 268 Query: 216 LGTGGMASKMSSALLAADA-GVPVLLAPAADAAAALTDASVGTV 258 G M K+ +A+ + G ++A A AL + GT Sbjct: 269 FKAGSMGPKVLAAIRFIEWGGERAIIAHLEKAVEAL-EGKTGTQ 311
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_3712	SECA	32	0.007	SecA protein signature.
>SECA#SecA protein signature. Length = 901 Score = 31.8 bits (72), Expect = 0.007 Identities = 21/101 (20%), Positives = 40/101 (39%), Gaps = 29/101 (28%) Query: 387 IGQTNFDNDEAVGYLADRLVRLGVEEELL---------RLGAKPGC--AVTIGEMTFDWE 435 +G + + E +++ L + G++ +L + A+ G AVTI Sbjct: 454 VGTISIEKSE---LVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIA------- 503 Query: 436 PQTPAGGHVAMSGRGTDVRLERSDRVGAAERKAARRQRRER 476 M+GRGTD+ L S + A + ++ E+ Sbjct: 504 --------TNMAGRGTDIVLGGSWQAEVAALENPTAEQIEK 536

MUL_3950

MUL_3956

Genomic Island

LocusTag	DNBias	CDNBias	%GCBias	Product
MUL_3950	2	15	-2.245557	cytochrome P450 187A5 Cyp187A5
MUL_3951	4	16	-2.271836	dehydratase
MUL_3952	4	16	-1.757037	acyl-CoA dehydrogenase
MUL_3953	4	16	-1.951414	metal-dependent hydrolase
MUL_3954	3	17	-1.835101	metal-dependent hydrolase
MUL_3955	4	15	-2.321184	enoyl-CoA hydratase
MUL_3956	4	15	-2.215232	hypothetical protein

MUL_4174

MUL_4186

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
MUL_4174	1	14	-3.265609	hypothetical protein
MUL_4176	1	12	-3.206807	acyl-CoA dehydrogenase FadE29
MUL_4178	1	11	-2.996047	acyl-CoA dehydrogenase FadE28
MUL_4179	1	17	-3.604760	cytochrome P450 125A7 Cyp125A7
MUL_4180	1	17	-2.149123	acetyl-CoA acetyltransferase
MUL_4181	-1	15	-0.583813	hypothetical protein
MUL_4182	-1	15	1.679706	short chain dehydrogenase
MUL_4183	-1	13	2.632367	short chain dehydrogenase
MUL_4184	-1	12	3.071904	enoyl-CoA hydratase
MUL_4185	0	13	3.445190	CoA-transferase subunit alpha
MUL_4186	-2	13	3.221310	CoA-transferase subunit beta

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_4178	HTHFIS	35	0.001	FIS bacterial regulatory protein HTH signature.
>HTHFIS#FIS bacterial regulatory protein HTH signature. Length = 484 Score = 35.2 bits (81), Expect = 0.001 Identities = 32/160 (20%), Positives = 59/160 (36%), Gaps = 28/160 (17%) Query: 518 IIGQEDAVKAVSKAIRRTRAGLKDPKRPSGSFIFAGPSGVGKTELSKALANFLFGDDDAL 577 ++G+ A++ + + + R + + + G SG GK +++AL ++ + Sbjct: 139 LVGRSAAMQEIYRVLARL-------MQTDLTLMITGESGTGKELVARALHDYGKRRNGPF 191 Query: 578 IQIDMGEFHDRFTASRLFGAPPGYVGYEEGGQLTEKVRRKP--FSVA-----LFDEIEKA 630 + I+M S LFG E G T R F A DEI Sbjct: 192 VAINMAAIPRDLIESELFGH--------EKGAFTGAQTRSTGRFEQAEGGTLFLDEIGDM 243 Query: 631 HQEISNSLLQVLEDG---RLTDGQGRTVDFKNTVLIFTSN 667 + LL+VL+ G + D + ++ +N Sbjct: 244 PMDAQTRLLRVLQQGEYTTVGGRTPIRSDVR---IVAATN 280
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_4179	IGASERPTASE	30	0.016	IgA-specific serine endopeptidase (S6) signature.
>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature. Length = 1541 Score = 30.0 bits (67), Expect = 0.016 Identities = 17/51 (33%), Positives = 21/51 (41%), Gaps = 3/51 (5%) Query: 283 VTFDEDRHRAHTGNGAQVLATLRNTAINLHRLNGADNIADACRITALTANR 333 V E+ H TGN L N I+ LN ADN + + LT N Sbjct: 846 VRLTENSHWHLTGNSDVHQLDLANGHIH---LNSADNSNNVTKYNTLTVNS 893
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_4182	PF03309	360	e-129	Bvg accessory factor
>PF03309#Bvg accessory factor Length = 271 Score = 360 bits (926), Expect = e-129 Identities = 239/272 (87%), Positives = 256/272 (94%), Gaps = 1/272 (0%) Query: 1 MLLAIDVRNTHTVVGLLSGAKQHAKVVQQWRIRTESEVTADELALTIDGLIGEDSERLTG 60 MLLAIDVRNTHTVVGL+SG+ HAKVVQQWRIRTE EVTADELALTIDGLIG+D+ERLTG Sbjct: 1 MLLAIDVRNTHTVVGLISGSGDHAKVVQQWRIRTEPEVTADELALTIDGLIGDDAERLTG 60 Query: 61 ATGLSTVPSVLHEVRIMLEQYWPSVPHVLIEPGVRTGIPLLVDNPKEVGADRIVNCLAAY 120 A+GLSTVPSVLHEVR+MLEQYWP+VPHVLIEPGVRTGIPLLVDNPKEVGADRIVNCLAAY Sbjct: 61 ASGLSTVPSVLHEVRVMLEQYWPNVPHVLIEPGVRTGIPLLVDNPKEVGADRIVNCLAAY 120 Query: 121 QQFAKAAIVVDFGSSICVDVVSAKGEFLGGAIAPGVQVSSDAAAARSAALRRVELARPRS 180 ++ AAIVVDFGSSICVDVVSAKGEFLGGAIAPGVQVSSDAAAARSAALRRVEL RPRS Sbjct: 121 HKYGTAAIVVDFGSSICVDVVSAKGEFLGGAIAPGVQVSSDAAAARSAALRRVELTRPRS 180 Query: 181 VVGKNTVECMQAGAVFGFAGLVDGLVARIREDVKGFSADDDVAVVATGHTAPLLLPELHS 240 V+GKNTVECMQAGAVFGFAGLVDGLV RIR+DV GFS DVAVVATGHTAPL+LP+L + Sbjct: 181 VIGKNTVECMQAGAVFGFAGLVDGLVNRIRDDVDGFS-GADVAVVATGHTAPLVLPDLRT 239 Query: 241 VEHFDEHLTLNGLRLVFERNREAQRGRLKPAR 272 VEH+D HLTL+GLRLVFERNR QRG+LKPAR Sbjct: 240 VEHYDRHLTLDGLRLVFERNRANQRGKLKPAR 271
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_4186	PF05616	30	0.016	Neisseria meningitidis TspB protein
>PF05616#Neisseria meningitidis TspB protein Length = 501 Score = 30.5 bits (68), Expect = 0.016 Identities = 30/115 (26%), Positives = 45/115 (39%), Gaps = 15/115 (13%) Query: 336 GRRSRSRHSTDYRDYGVGRLGAGPPPGPGPAQPPPMASAAPPWPGPQPAEPAAPRLAPPP 395 GR S+ + D + L G P P ++ A P P P E R P P Sbjct: 296 GRDSQGNTTVDVQVIPRPDLTPGSAEAPNAQPLPEVSPAENPANNPAPNENPGTRPNPEP 355 Query: 396 APDLASRHRRPEEERPDVAGSHDSDSQSGGQSVADLMARLQVQPSGGGRRRRRDG 450 PDL PD + D+D Q G + + + +P+G R+ R++G Sbjct: 356 DPDL----------NPD--ANPDTDGQPGTRPDSPAVPD---RPNGRHRKERKEG 395

MUL_4221

MUL_4232

Genomic Island

LocusTag	DNBias	CDNBias	%GCBias	Product
MUL_4221	2	9	0.311477	lipoprotein LppH
MUL_4222	3	10	-0.348469	lipoprotein
MUL_4223	1	18	0.236046	LamB/YcsF family protein
MUL_4224	1	17	0.961284	hypothetical protein
MUL_4226	2	15	2.723540	arsenical pump integral membrane protein ArsB2
MUL_4228	3	15	4.252459	tRNA/rRNA methyltransferase
MUL_4229	2	12	3.416609	cysteinyl-tRNA synthetase
MUL_4230	2	13	3.314523	2-C-methyl-D-erythritol 2,4-cyclodiphosphate
MUL_4231	1	11	3.783277	2-C-methyl-D-erythritol 4-phosphate
MUL_4232	0	11	3.305044	transcriptional regulatory protein

MUL_4258

MUL_4280

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
MUL_4258	1	19	4.046740	dihydroneopterin aldolase FolB
MUL_4259	1	16	3.110306	dihydropteroate synthase 1 FolP1
MUL_4261	4	16	4.156746	GTP cyclohydrolase I
MUL_4263	3	14	3.739659	membrane-bound protease FtsH
MUL_4264	2	13	3.607618	epoxide hydrolase EphA
MUL_4270	2	12	3.437809	monooxygenase
MUL_4271	0	12	3.042699	PPE family protein
MUL_4272	0	12	3.103988	PE family protein
MUL_4273	0	13	2.965435	transposase for IS2404
MUL_4274	0	13	3.057403	lipoprotein LpqG
MUL_4275	2	14	5.904588	hypoxanthine-guanine phosphoribosyltransferase
MUL_4276	2	14	5.238496	cell cycle protein MesJ
MUL_4277	1	13	5.084098	hypothetical protein
MUL_4278	0	14	4.805945	D-alanyl-D-alanine carboxypeptidase
MUL_4279	1	13	4.333477	inorganic pyrophosphatase Ppa
MUL_4280	0	12	4.382156	hypothetical protein

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_4272	BACINVASINB	38	5e-05	Salmonella/Shigella invasin protein B signature.
>BACINVASINB#Salmonella/Shigella invasin protein B signature. Length = 593 Score = 38.2 bits (88), Expect = 5e-05 Identities = 35/140 (25%), Positives = 65/140 (46%), Gaps = 21/140 (15%) Query: 236 LAGLVVVILVGVAAAANGATAALLGFPLVLLVGLLVAYLYTVLMFA-----PVL-IVLER 289 L L+ ++ V A GA+ AL L ++V + T + F P++ VL+ Sbjct: 321 LGALLTIVSVVAAVFTGGASLALAAVGLAVMVADEIVKAATGVSFIQQALNPIMEHVLK- 379 Query: 290 LPLVDAITRSFALVTGGFWRVLGIRLLTAIVVGLVGGAISAPFGIVGQILLGATASEGST 349 PL++ I ++ G LG+ TA + G + GAI A +V I++ A +G+ Sbjct: 380 -PLMELIGKAITKALEG----LGVDKKTAEMAGSIVGAIVAAIAMVAVIVVVAVVGKGAA 434 Query: 350 GMFLVGMTLSSIGSAISQII 369 + +G+A+S+++ Sbjct: 435 ---------AKLGNALSKMM 445
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_4275	HTHFIS	38	5e-05	FIS bacterial regulatory protein HTH signature.
>HTHFIS#FIS bacterial regulatory protein HTH signature. Length = 484 Score = 37.5 bits (87), Expect = 5e-05 Identities = 44/207 (21%), Positives = 67/207 (32%), Gaps = 22/207 (10%) Query: 117 DEINRTPPKTQAALLEAMEERQVSVEGQAKPLP-DPFIVAATQNPIEYEGTHQLPEAQLD 175 DEI P Q LL +++ + + G P+ D IVAAT ++ L L Sbjct: 238 DEIGDMPMDAQTRLLRVLQQGEYTTVGGRTPIRSDVRIVAATNKDLKQSINQGLFREDL- 296 Query: 176 RFLLKLNVG---LPS---RESEIAILGRH-----------AHGFDPRDLSAIKPVAGPAE 218 +LNV LP R +I L RH FD L +K P Sbjct: 297 --YYRLNVVPLRLPPLRDRAEDIPDLVRHFVQQAEKEGLDVKRFDQEALELMKAHPWPGN 354 Query: 219 LAAGREAVSRVLIADEVLGYIVDIVGATRSSPALQLGVSPRGATALLGTARSWAWLSGRN 278 + V R+ +I+ S + A + + + R Sbjct: 355 VRELENLVRRLTALYPQDVITREIIENELRSEIPDSPIEKAAARSGSLSISQAVEENMRQ 414 Query: 279 YVTPDDVKAMARPTLRHRIMLRLEAEL 305 Y A+ L R++ +E L Sbjct: 415 Y-FASFGDALPPSGLYDRVLAEMEYPL 440
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_4278	PERTACTIN	32	0.004	Pertactin signature.
>PERTACTIN#Pertactin signature. Length = 922 Score = 31.6 bits (71), Expect = 0.004 Identities = 18/45 (40%), Positives = 19/45 (42%) Query: 248 GAGAPPGWPPQTPPAPVWWPGQPAPQPLIQPPFAPDPAPSPPQGP 292 GA APP P P P P P P QPP P P P+ P Sbjct: 564 GAKAPPAPKPAPQPGPQPGPQPPQPPQPPQPPQPPQPPQRQPEAP 608
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_4279	cloacin	29	0.020	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 28.9 bits (64), Expect = 0.020 Identities = 15/37 (40%), Positives = 16/37 (43%) Query: 76 PLGFGGGFGPGFGPGLGFGFGPGGARGGGRRGGPGRG 112 P G G G G +G G G G G G GG G G Sbjct: 45 PWGGGSGSGIHWGGGSGHGNGGGNGNSGGGSGTGGNL 81
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_4280	cloacin	39	5e-05	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 39.3 bits (91), Expect = 5e-05 Identities = 43/112 (38%), Positives = 50/112 (44%), Gaps = 11/112 (9%) Query: 536 GDGGGGGGGASGGGGGASGGTGGTGGAGGLLSAGGAGGVGGAGGYNTSGPGGNGGSGGNA 595 GDG G GA G +GG G G GG G+G + + P G GGSG Sbjct: 4 GDGRGHNTGAHSTSGNINGGPTGLGVGGG--------ASDGSGWSSENNPWG-GGSGSGI 54 Query: 596 GTLFGSGGGGNGGSGYSGIGGTGGTGGNAVLLGGGGAGGAGGISFTGAGGQG 647 GSG G GG+G SG G GTGGN + A G +S GAGG Sbjct: 55 HWGGGSGHGNGGGNGNSG--GGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 38.9 bits (90), Expect = 8e-05 Identities = 46/130 (35%), Positives = 54/130 (41%), Gaps = 13/130 (10%) Query: 147 GAGGAGGAGGAGGSSTGGAGGTGGAGGAGEWLFGPGGVGGAGGSSSSAGGAGGVGGAGGL 206 G G GA G+ GG G G GGA + G SS GG G+G Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGGASD----------GSGWSSENNPWGGGSGSGIH 55 Query: 207 FGGGLGGAGGAGVSASGGAGGAGGAGGALAGFLGAGG---SDGGAGGTGVNHEGGAGGAG 263 +GGG G G G SGG G GG A+A + G S GAGG V+ GA A Sbjct: 56 WGGGSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAA 115 Query: 264 GAGGLIAGTG 273 A + A G Sbjct: 116 IADIMAALKG 125 Score = 38.2 bits (88), Expect = 1e-04 Identities = 37/116 (31%), Positives = 41/116 (35%), Gaps = 6/116 (5%) Query: 123 GNDGAGGSGAAGSAGGAGGAAGLIGAGGAGGAGGAGGSSTGGAGGTGGAGGAGEWLFGPG 182 G DG G + A S G G G + G+G SS G G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 183 GVGGAGGSSSSAGGAGGVGG------AGGLFGGGLGGAGGAGVSASGGAGGAGGAG 232 G GG G+S G GG A G GAGG VS S GA A A Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAAIAD 118 Score = 34.7 bits (79), Expect = 0.001 Identities = 31/100 (31%), Positives = 37/100 (37%), Gaps = 3/100 (3%) Query: 628 GGGGAGGAGGISFTGAGGQGGAGGTGGQLSGNGGSGGTGGEGDYVGGADSGGAGGAGGNA 687 GG G G G T GG G G + GSG + + GG+ SG G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 688 GLTGDGGNGGSGGTPGSPGGGGTGGALIGQDGLARLAVTG 727 G G GN G G GG + A G L+ G Sbjct: 63 GNGGGNGNSGGG---SGTGGNLSAVAAPVAFGFPALSTPG 99 Score = 33.9 bits (77), Expect = 0.002 Identities = 33/88 (37%), Positives = 41/88 (46%), Gaps = 8/88 (9%) Query: 570 GAGGVGGAGGYNTSGPGGNGGSGGNAGTLFGSGGGGNGGSGYSGIGGTGGTGGNAVLLGG 629 G G G G +++ NGG G G GGG + GSG+S G G + + G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTG-----LGVGGGASDGSGWSSENNPWGGGSGSGIHWG 57 Query: 630 GGAGGAGGISFTGAGGQGGAGGTGGQLS 657 GG+G G G G GG GTGG LS Sbjct: 58 GGSGHGNG---GGNGNSGGGSGTGGNLS 82 Score = 33.1 bits (75), Expect = 0.004 Identities = 32/101 (31%), Positives = 42/101 (41%), Gaps = 7/101 (6%) Query: 243 GSDGGAGGTGVNHEGGAGGAGGAGGLIAGTG------GNGGAGGTDAYSRGGAGGAGGTG 296 G + GA T N GG G G GG G+G GG G+ + GG+G G G Sbjct: 8 GHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGG 67 Query: 297 GTGGTDMSDSGGTGGAGGNA-GLLFGSGGAGGAGGAAVALN 336 S +GG A F + GAGG AV+++ Sbjct: 68 NGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSIS 108 Score = 33.1 bits (75), Expect = 0.004 Identities = 32/95 (33%), Positives = 38/95 (40%) Query: 497 GDGGAAGLLGTGGTGGAGARLAGGAGGTGGAGGAGGWLLGDGGGGGGGASGGGGGASGGT 556 G +G + G TG A G G G G GGG+ G GG +G + Sbjct: 12 GAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNGNS 71 Query: 557 GGTGGAGGLLSAGGAGGVGGAGGYNTSGPGGNGGS 591 GG G GG LSA A G +T G GG S Sbjct: 72 GGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVS 106 Score = 32.8 bits (74), Expect = 0.006 Identities = 29/82 (35%), Positives = 34/82 (41%), Gaps = 5/82 (6%) Query: 569 GGAGGVGGAGGYNTSGPGGNG-GSGGNAGTLFGSGGGGNGGSGYSGIGGTGGTGGNAVLL 627 G G G GP G G G G + G+ + S GG SGI GG+G Sbjct: 8 GHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHG---- 63 Query: 628 GGGGAGGAGGISFTGAGGQGGA 649 GGG G +GG S TG A Sbjct: 64 NGGGNGNSGGGSGTGGNLSAVA 85 Score = 32.4 bits (73), Expect = 0.006 Identities = 31/93 (33%), Positives = 41/93 (44%), Gaps = 9/93 (9%) Query: 248 AGGTGVNHEGGAGGA-----GGAGGLIAGTGGNGGAGGTDAYSRGGAGGAGGTGGTGGTD 302 +GG G H GA GG GL G G + G+G + + G G G GG+ Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 303 MSDSGGTGGAGGNAGLLFGSGGAGGAGGAAVAL 335 + GG G +GG + G+GG A A VA Sbjct: 62 HGNGGGNGNSGGGS----GTGGNLSAVAAPVAF 90 Score = 32.4 bits (73), Expect = 0.008 Identities = 24/79 (30%), Positives = 31/79 (39%) Query: 483 AGSGTDGTPGGWLLGDGGAAGLLGTGGTGGAGARLAGGAGGTGGAGGAGGWLLGDGGGGG 542 +G G G G G G GG + +G + GG G + GGG G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 543 GGASGGGGGASGGTGGTGG 561 G GG G + GG+G G Sbjct: 62 HGNGGGNGNSGGGSGTGGN 80 Score = 31.2 bits (70), Expect = 0.014 Identities = 28/84 (33%), Positives = 35/84 (41%), Gaps = 4/84 (4%) Query: 600 GSGGGGNGGSGYSGIGGTGGTGGNAVLLGGGGAGGAGGISFTGAGGQGGAGGTGGQLSGN 659 G G G N G+ + GG G LG GG G + GG G+G G Sbjct: 4 GDGRGHNTGAHSTSGNINGGPTG----LGVGGGASDGSGWSSENNPWGGGSGSGIHWGGG 59 Query: 660 GGSGGTGGEGDYVGGADSGGAGGA 683 G G GG G+ GG+ +GG A Sbjct: 60 SGHGNGGGNGNSGGGSGTGGNLSA 83 Score = 31.2 bits (70), Expect = 0.015 Identities = 37/109 (33%), Positives = 47/109 (43%), Gaps = 3/109 (2%) Query: 203 AGGLFGGGLGGAGGAGVSASGGAGGAGGAGGAL--AGFLGAGGSDGGAGGTGVNHEGGAG 260 +GG G GA + +GG G G GGA +G+ GG G+G++ GG+G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 261 -GAGGAGGLIAGTGGNGGAGGTDAYSRGGAGGAGGTGGTGGTDMSDSGG 308 G GG G G G GG A A T G GG +S S G Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAG 110 Score = 31.2 bits (70), Expect = 0.017 Identities = 35/115 (30%), Positives = 41/115 (35%), Gaps = 6/115 (5%) Query: 298 TGGTDMSDSGGTGGAGGNAGLLFGSGGAGGAGGAAVALNDVGGAGGAGGNAGLFGNGGVG 357 +GG + G GN G G G GGA +D G G G+ Sbjct: 2 SGGDGRGHNTGAHSTSGNIN--GGPTGLGVGGGA----SDGSGWSSENNPWGGGSGSGIH 55 Query: 358 GVGGVGAGDGGAGGRAGLVIGNGGAGGAGGESFGFGAGVGGAGGNGGNGVLIGNG 412 GG G G+GG G +G G GG A FG G GG V I G Sbjct: 56 WGGGSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAG 110 Score = 30.1 bits (67), Expect = 0.038 Identities = 28/78 (35%), Positives = 34/78 (43%), Gaps = 4/78 (5%) Query: 271 GTGGNGGAGGTDAYSRGGAGGAGGTGG-TGGTDMSDSGGTGGAGGNAGLLFGSGGAGGAG 329 G G N GA T GG G G GG + G+ S G G +G+ +G G G G Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNG 65 Query: 330 GAAVALNDVGGAGGAGGN 347 G + GG G GGN Sbjct: 66 GGN---GNSGGGSGTGGN 80 Score = 30.1 bits (67), Expect = 0.041 Identities = 33/98 (33%), Positives = 40/98 (40%), Gaps = 7/98 (7%) Query: 340 GAGGAGGNAGLFGNGGV--GGVGGVGAGDGGAGGRAGLVIGNGGAGGAGGESFGFGAGVG 397 G G G N G G GG G+G G G + G N GG+G G G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSG---SGIHWGGG 59 Query: 398 GAGGNGGNGVLIGNGGNAGTGGTGLSTGSTGAGGISGL 435 GNGG +GG +GTGG + + A G L Sbjct: 60 SGHGNGGGNG--NSGGGSGTGGNLSAVAAPVAFGFPAL 95

MUL_4362

MUL_4399

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
MUL_4362	5	21	2.816649	non-IS element not present in Mycobacterium
MUL_4363	7	23	2.215993	non-IS element not present in Mycobacterium
MUL_4364	9	27	3.182163	glycerol kinase
MUL_4365	7	26	3.058058	selenocysteine lyase, CsdB
MUL_4366	7	24	3.336049	S-adenosyl-L-methionine-
MUL_4367	5	24	3.170061	hypothetical protein
MUL_4368	-3	13	-0.633453	hypothetical protein
MUL_4370	-1	13	0.322066	hypothetical protein
MUL_4371	-1	13	-0.691480	catalase KatE
MUL_4372	-1	14	-0.396843	hypothetical protein
MUL_4373	0	16	-0.812059	hypothetical protein
MUL_4374	5	14	3.968215	hypothetical protein
MUL_4375	3	13	4.118395	aspartate-semialdehyde dehydrogenase
MUL_4377	3	12	4.153867	aspartate kinase
MUL_4378	3	13	4.217472	hypothetical protein
MUL_4380	2	13	3.973379	2-isopropylmalate synthase
MUL_4381	2	13	3.973918	DeoR family transcriptional regulator
MUL_4383	-1	11	0.180498	hypothetical protein
MUL_4384	-1	10	-0.342069	DNA polymerase III subunit epsilon
MUL_4385	0	10	-0.794217	UDP-N-acetylmuramyl tripeptide synthase
MUL_4386	0	10	-1.526386	cobyric acid synthase CobQ2
MUL_4387	0	9	-1.798722	recombination protein RecR
MUL_4388	-1	13	-3.009572	hypothetical protein
MUL_4389	-1	17	-3.810290	N-acetylmuramoyl-L-alanine amidase
MUL_4391	2	17	-1.396148	hypothetical protein
MUL_4392	5	17	0.428900	dehydrogenase
MUL_4394	5	17	0.560383	hypothetical protein
MUL_4397	4	16	0.792042	hypothetical protein
MUL_4398	4	16	1.721189	hypothetical protein
MUL_4399	4	14	2.718756	DNA polymerase III subunits gamma and tau

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_4364	PERTACTIN	31	0.002	Pertactin signature.
>PERTACTIN#Pertactin signature. Length = 922 Score = 30.8 bits (69), Expect = 0.002 Identities = 23/58 (39%), Positives = 25/58 (43%), Gaps = 11/58 (18%) Query: 14 PPWVGQPYGQPGPNPQFPPSWGYPAQPGGQPQPYPGNPSYPYPNFAQAPEPQAPFGRD 71 P QP QPGP P PP P QP PQ P +AP PQ P GR+ Sbjct: 571 PKPAPQPGPQPGPQPPQPPQPPQPPQPPQPPQRQP-----------EAPAPQPPAGRE 617
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_4367	FLAGELLIN	40	3e-05	Flagellin signature.
>FLAGELLIN#Flagellin signature. Length = 507 Score = 40.4 bits (94), Expect = 3e-05 Identities = 43/300 (14%), Positives = 69/300 (23%), Gaps = 2/300 (0%) Query: 639 GSAAFNGVNGAGGAGGNGGAGGGGGAGASGIGAGVAGGDGGTGGTGGAAGTGGAAGTGGT 698 FNGV + N G I + D + G G G T G Sbjct: 127 NQTQFNGVKVL--SQDNQMKIQVGANDGETITIDLQKIDVKSLGLDGFNVNGPKEATVGD 184 Query: 699 GGAAGAAGSAGDGGAGGAGGAGDDGSAGVVSGDPGTAGKAGGAGGAGGVGGNGVAGGVNG 758 ++ + D A GA D ++G V D G N Sbjct: 185 LKSSFKNVTGYDTYAVGANKYRVDVNSGAVVTDTTAPTVPDKVYVNAANGQLTTDDAENN 244 Query: 759 SGGAGGAGGAGGTGGAGASGVPASVDGGDGGAGGAGGAAGTGGSGGSAGNTGAGGAGGAG 818 + G A A + ++ GG G + + + Sbjct: 245 TAVDLFKTTKSTAGTAEAKAIAGAIKGGKEGDTFDYKGVTFTIDTKTGNDGNGKVSTTIN 304 Query: 819 GAGGSAASTGVNGDGGAGGAGGVGGLGGDSAATAGGGGAGGDGGKGGAGGAGVNGVGGGD 878 G + + A + + G D K + Sbjct: 305 GEKVTLTVADITAGAANVDAATLQSSKNVYTSVVNGQFTFDDKTKNESAKLSDLEANNAV 364 Query: 879 GGKGGTGGAGGAGGAGSDGIIPSNGGLGGNGGNGGNGGQAFGDGVGGAGGIGGKGGNAAI 938 G+ G A + G + G +G + A A+I Sbjct: 365 KGESKITVNGAEYTANAAGDKVTLAGKTMFIDKTASGVSTLINEDAAAAKKSTANPLASI 424
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_4370	SSPANPROTEIN	27	0.040	Salmonella invasion protein InvJ signature.
>SSPANPROTEIN#Salmonella invasion protein InvJ signature. Length = 336 Score = 26.6 bits (58), Expect = 0.040 Identities = 24/79 (30%), Positives = 36/79 (45%), Gaps = 5/79 (6%) Query: 14 AAATAGPRDVPIGAVMISADGTELARAVNAREELGDPTAHA---EILALRAAARVL-GDG 69 +AA ++ P+ +V +L +AV + E+ D I AL + + G+G Sbjct: 120 SAALLSSKNRPLESVSGKKLSADL-KAVESVSEVTDNATGISDDNIKALPGDNKAIAGEG 178 Query: 70 WRLEGATLAVTVEPCTMCA 88 R EGA LA V P M A Sbjct: 179 VRKEGAPLARDVAPARMAA 197
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_4381	cloacin	40	5e-05	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 39.7 bits (92), Expect = 5e-05 Identities = 27/85 (31%), Positives = 37/85 (43%), Gaps = 2/85 (2%) Query: 517 NGGNGGKGANGNAALGANRNGGNGGIGGTGFIGGNGGNGGGGGAGGNGGNGAAGVSAGGA 576 +GG+G G + N NGG G+G G G + G+G GG +G+ GG Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGG--GASDGSGWSSENNPWGGGSGSGIHWGGG 59 Query: 577 GGAGGEGNGGTAGTGGKGGDGGTAS 601 G G G G +G G G +A Sbjct: 60 SGHGNGGGNGNSGGGSGTGGNLSAV 84 Score = 38.9 bits (90), Expect = 9e-05 Identities = 31/91 (34%), Positives = 38/91 (41%), Gaps = 3/91 (3%) Query: 426 LGGNGGHGGVGGGHTAGNGFNDGTTGAGGVGGVGGTGGVGGTG---GDGLHTALGRQGNG 482 + G G G G H+ N G TG G GG G G G + + G Sbjct: 1 MSGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGS 60 Query: 483 GAGGHGGSGNQGGYGGLSGDESSRAAQGATG 513 G G GG+GN GG G G+ S+ AA A G Sbjct: 61 GHGNGGGNGNSGGGSGTGGNLSAVAAPVAFG 91 Score = 37.8 bits (87), Expect = 2e-04 Identities = 28/80 (35%), Positives = 33/80 (41%) Query: 784 GQFNGGNGGKGGVGGTAGAGTTAGNGGHGGTGGTGGDGQTAAGVGGSGGTGGNGGRGGTG 843 G G N G G G T G G + G+G + GGSG GG G G Sbjct: 4 GDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHG 63 Query: 844 GAGGTGVTGGDGGRGGSGGA 863 GG G +GG G GG+ A Sbjct: 64 NGGGNGNSGGGSGTGGNLSA 83 Score = 37.4 bits (86), Expect = 3e-04 Identities = 30/89 (33%), Positives = 38/89 (42%) Query: 486 GHGGSGNQGGYGGLSGDESSRAAQGATGNDGNGGNGGKGANGNAALGANRNGGNGGIGGT 545 G G G+ G SG+ + G + G+G N G+ GG G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 546 GFIGGNGGNGGGGGAGGNGGNGAAGVSAG 574 G GGNG +GGG G GGN AA V+ G Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFG 91 Score = 36.6 bits (84), Expect = 5e-04 Identities = 30/85 (35%), Positives = 37/85 (43%), Gaps = 1/85 (1%) Query: 741 AGGAGANETYGGSGGSGGAAGNGGVGGLNGVGGNGGVGGHGGNGQFNGGNGGKGGVGGTA 800 +GG G G SG G G+ G G + G G N + GG+G GG + Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGG-GASDGSGWSSENNPWGGGSGSGIHWGGGS 60 Query: 801 GAGTTAGNGGHGGTGGTGGDGQTAA 825 G G GNG GG GTGG+ A Sbjct: 61 GHGNGGGNGNSGGGSGTGGNLSAVA 85 Score = 36.2 bits (83), Expect = 6e-04 Identities = 28/79 (35%), Positives = 31/79 (39%), Gaps = 1/79 (1%) Query: 810 GHGGTGGTGGDGQTAAGV-GGSGGTGGNGGRGGTGGAGGTGVTGGDGGRGGSGGAGGDGA 868 G G G G T+ + GG G G GG G G G G GG G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 869 GTCAGNGGNGGLGGAGGNG 887 G GNG +GG G GGN Sbjct: 63 GNGGGNGNSGGGSGTGGNL 81 Score = 35.8 bits (82), Expect = 7e-04 Identities = 33/104 (31%), Positives = 38/104 (36%), Gaps = 7/104 (6%) Query: 627 NVGGHGGNGGTGGAAGTGGHGGNGGDGGNGGINANGAAGGAGGNGGAGGGSTLLANGHGG 686 N G H +G G G GG DG N GG+G GGGS G G Sbjct: 10 NTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNG 69 Query: 687 AGGNGGNGGIGGENASNRGATG-------GAGGVGGIGGAGSLS 723 G G G + A G GAGG+ AG+LS Sbjct: 70 NSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALS 113 Score = 35.5 bits (81), Expect = 0.001 Identities = 27/86 (31%), Positives = 34/86 (39%), Gaps = 2/86 (2%) Query: 579 AGGEGNGGTAGTGGKGGDGGTASDDGVGGDGGSGGRGGDGNTRYTPRGNVGGHGGNGGTG 638 +GG+G G G G+ G G S G G ++ P G G G + G G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGW--SSENNPWGGGSGSGIHWGGG 59 Query: 639 GAAGTGGHGGNGGDGGNGGINANGAA 664 G GG GN G G G N + A Sbjct: 60 SGHGNGGGNGNSGGGSGTGGNLSAVA 85 Score = 35.5 bits (81), Expect = 0.001 Identities = 32/89 (35%), Positives = 36/89 (40%), Gaps = 6/89 (6%) Query: 512 TGNDGNGGNGGKGANGNAALGA------NRNGGNGGIGGTGFIGGNGGNGGGGGAGGNGG 565 T + NGG G G G A+ G+ N GG G G G GNGGG G G G Sbjct: 16 TSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNGNSGGGS 75 Query: 566 NGAAGVSAGGAGGAGGEGNGGTAGTGGKG 594 +SA A A G T G GG Sbjct: 76 GTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 35.5 bits (81), Expect = 0.001 Identities = 29/73 (39%), Positives = 32/73 (43%), Gaps = 8/73 (10%) Query: 829 GSGGTGGNGGRGGTGGAGGTGVTGGDGGRGGSGGAG--------GDGAGTCAGNGGNGGL 880 G G G N G T G G TG G G S G+G G G+G+ GG G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 881 GGAGGNGGHGGTS 893 G GGNG GG S Sbjct: 63 GNGGGNGNSGGGS 75 Score = 33.5 bits (76), Expect = 0.004 Identities = 26/89 (29%), Positives = 30/89 (33%) Query: 368 GVGGAGGEGGLIKGNGGAGGADGIGGTGGIGGDGSRGDTPLGPFNVDGHSGGDGGRGGLG 427 G G G G +G G G GG DGS + P+ SG G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 428 GNGGHGGVGGGHTAGNGFNDGTTGAGGVG 456 GNGG G GG + G G Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFG 91 Score = 33.1 bits (75), Expect = 0.005 Identities = 29/96 (30%), Positives = 37/96 (38%), Gaps = 10/96 (10%) Query: 548 IGGNGGNGGGGGAGGNGGNGAAGVSAGGAGGAGGEGNGGTAGTGGKGGDGGTASDDGVGG 607 + G G G GA GN G + G GG +G+G ++ GG G+ G G Sbjct: 1 MSGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGS 60 Query: 608 DGGSGGRGGDGNTRYTPRGNVGGHGGNGGTGGAAGT 643 G+GG GN GG G GG A Sbjct: 61 GHGNGGGN----------GNSGGGSGTGGNLSAVAA 86 Score = 33.1 bits (75), Expect = 0.005 Identities = 39/119 (32%), Positives = 46/119 (38%), Gaps = 8/119 (6%) Query: 709 GAGGVGGIGGAGSLS--IRGGGYGGLGGNGGTGGAGGAGANETYGGSGGSGGAAGNGGVG 766 G G G GA S S I GG G G G + G+G + N +GG GSG GG Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGG--GSGSGIHWGGGS 60 Query: 767 GLNGVGGNGGVGGHGGNGQFNGGNGGKGGVGGTAGAGTTAGNGGHGGTGGTGGDGQTAA 825 G GNGG G+ G G GGN G + G G +AA Sbjct: 61 G----HGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAA 115 Score = 32.8 bits (74), Expect = 0.007 Identities = 33/116 (28%), Positives = 42/116 (36%) Query: 630 GHGGNGGTGGAAGTGGHGGNGGDGGNGGINANGAAGGAGGNGGAGGGSTLLANGHGGAGG 689 G G G GA T G+ G G G A+ +G + N GGGS + GG+G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 690 NGGNGGIGGENASNRGATGGAGGVGGIGGAGSLSIRGGGYGGLGGNGGTGGAGGAG 745 G G S G A G +LS G G + + G A A Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAAIAD 118 Score = 32.4 bits (73), Expect = 0.010 Identities = 28/87 (32%), Positives = 35/87 (40%), Gaps = 5/87 (5%) Query: 538 GNGGIGGTGFIGGNGGNGGGGGAGGNGGNGAAGVSAGGAGGAGGEGNGGTAGTGGKGGDG 597 G G G GN G G G G + +G S+ GG G+G G G G+G Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNG 65 Query: 598 GTASDDGVGGDGGSGGRGGDGNTRYTP 624 G G G GG G GG+ + P Sbjct: 66 G-----GNGNSGGGSGTGGNLSAVAAP 87 Score = 31.6 bits (71), Expect = 0.014 Identities = 37/106 (34%), Positives = 40/106 (37%), Gaps = 22/106 (20%) Query: 641 AGTGGHGGNGGDGGNGGINANGAAGGAGGNGGAGGGSTLLANGHGGAGGNGGNGGIGGEN 700 +G G G N G G N NG G G GGA GS G EN Sbjct: 2 SGGDGRGHNTGAHSTSG-NINGGPTGLGVGGGASDGS-----------------GWSSEN 43 Query: 701 ASNRGATGGAGGVGGIGGAGSLSIRGGGYGGLGGNGGTGGAGGAGA 746 G +G GG G G+ GGG G GG GTGG A A Sbjct: 44 NPWGGGSGSGIHWGGGSGHGN----GGGNGNSGGGSGTGGNLSAVA 85 Score = 31.6 bits (71), Expect = 0.016 Identities = 27/80 (33%), Positives = 31/80 (38%), Gaps = 4/80 (5%) Query: 316 GRGSDGGAGGKGGNAGDYGHGGAGGTGGQGGTGGAGLTPGDKGFQGGLGGSGGVGGAGGE 375 GRG + GA GN G G G G+G + G G GG G G Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNG 65 Query: 376 GGLIKGNGGAGGADGIGGTG 395 G GNG +GG G GG Sbjct: 66 G----GNGNSGGGSGTGGNL 81 Score = 31.6 bits (71), Expect = 0.017 Identities = 32/100 (32%), Positives = 39/100 (39%), Gaps = 11/100 (11%) Query: 260 GDGGAGAPGAASFDPNVAGGAGGAGGDAGKIGDGGRGGDGGHGATGTAGDATHLEGGRGS 319 GDG GA S N+ GG G G G G +G + + GG GS Sbjct: 4 GDGRGHNTGAHSTSGNINGGPTGLG-----------VGGGASDGSGWSSENNPWGGGSGS 52 Query: 320 DGGAGGKGGNAGDYGHGGAGGTGGQGGTGGAGLTPGDKGF 359 GG G+ G+G +GG G GG A P GF Sbjct: 53 GIHWGGGSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGF 92 Score = 31.2 bits (70), Expect = 0.021 Identities = 26/78 (33%), Positives = 32/78 (41%), Gaps = 1/78 (1%) Query: 770 GVGGNGGVGGHGGNGQFNGGNGGKGGVGGTAGAGTTAGNGGHGGTGGTGGDGQTAAGVGG 829 G G G H +G NGG G GVGG A G+ + + GG+G G G Sbjct: 4 GDGRGHNTGAHSTSGNINGGPTG-LGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 830 SGGTGGNGGRGGTGGAGG 847 G G GG+G G Sbjct: 63 GNGGGNGNSGGGSGTGGN 80 Score = 31.2 bits (70), Expect = 0.021 Identities = 29/82 (35%), Positives = 31/82 (37%), Gaps = 2/82 (2%) Query: 682 NGHGGAGGNGGNGGIGGENASNRGATGGAGGVGGIGGAGSLSIRGGGYGGLGGNGGTGGA 741 +G G G N G G N G TG G G G+G S GG G GG Sbjct: 2 SGGDGRGHNTGAHSTSGN--INGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGG 59 Query: 742 GGAGANETYGGSGGSGGAAGNG 763 G G G SGG G GN Sbjct: 60 SGHGNGGGNGNSGGGSGTGGNL 81 Score = 30.8 bits (69), Expect = 0.025 Identities = 27/101 (26%), Positives = 34/101 (33%) Query: 568 AAGVSAGGAGGAGGEGNGGTAGTGGKGGDGGTASDDGVGGDGGSGGRGGDGNTRYTPRGN 627 + G G GA G G G GG + G + G G + Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 628 VGGHGGNGGTGGAAGTGGHGGNGGDGGNGGINANGAAGGAG 668 G GGNG +GG +GTGG+ G A G G Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG 102 Score = 30.8 bits (69), Expect = 0.032 Identities = 30/86 (34%), Positives = 37/86 (43%), Gaps = 6/86 (6%) Query: 792 GKGGVGGTAGAGTTAGNGGHGGTGGTGGDGQTAAGVGGSGGTGGNGGRGGTGGAGGTGVT 851 G G G GA +T+GN +GG G G G + G G S GG G+G Sbjct: 3 GGDGRGHNTGAHSTSGNI-NGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIH-----W 56 Query: 852 GGDGGRGGSGGAGGDGAGTCAGNGGN 877 GG G G GG G G G+ G + Sbjct: 57 GGGSGHGNGGGNGNSGGGSGTGGNLS 82 Score = 30.1 bits (67), Expect = 0.042 Identities = 27/83 (32%), Positives = 33/83 (39%), Gaps = 2/83 (2%) Query: 814 TGGTGGDGQTAAGVGGSGGTGGNGGRGGTGGA--GGTGVTGGDGGRGGSGGAGGDGAGTC 871 +GG G T A GG G G GGA G + + GGSG G G+ Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 872 AGNGGNGGLGGAGGNGGHGGTST 894 GNGG G G G G ++ Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAV 84
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_4384	DHBDHDRGNASE	79	2e-19	2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase signature. Length = 261 Score = 78.6 bits (193), Expect = 2e-19 Identities = 68/254 (26%), Positives = 104/254 (40%), Gaps = 22/254 (8%) Query: 14 VVLITGGSRGLGRQMAFAAARCGANVVIASRNLDNCVATATEIESETGRSALAYQVHVGR 73 + ITG ++G+G +A A GA++ N + + R A A+ V Sbjct: 10 IAFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSS-LKAEARHAEAFPADVRD 68 Query: 74 WDQLDGLVAASYERFGKIDTLINNAG---MSPLYDKLSDVTEKLFDAVLNLNLKGPFRLS 130 +D + A G ID L+N AG + LSD + ++A ++N G F S Sbjct: 69 SAAIDEITARIEREMGPIDILVNVAGVLRPGLI-HSLSD---EEWEATFSVNSTGVFNAS 124 Query: 131 ALVGERMVAADGGSIINVSSAGSLRPSADIIPYAAAKAGLNAMTEGLARAFGPT-VRVNT 189 V + M+ GSI+ V S + P + YA++KA T+ L +R N Sbjct: 125 RSVSKYMMDRRSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLELAEYNIRCNI 184 Query: 190 LMAGPFLTDVSKAWNL----DAATQNPFGHLA-------LRRAGNPPEIVGAALFLASDA 238 + G T+ W+L + A Q G L L++ P +I A LFL S Sbjct: 185 VSPGS--TETDMQWSLWADENGAEQVIKGSLETFKTGIPLKKLAKPSDIADAVLFLVSGQ 242 Query: 239 SSFTTGSILRADGG 252 + T L DGG Sbjct: 243 AGHITMHNLCVDGG 256
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_4386	HTHTETR	51	3e-10	TetR bacterial regulatory protein HTH signature.
>HTHTETR#TetR bacterial regulatory protein HTH signature. Length = 215 Score = 50.8 bits (121), Expect = 3e-10 Identities = 24/121 (19%), Positives = 43/121 (35%), Gaps = 2/121 (1%) Query: 16 RQREATEEVERILAAAVRVMERAAPEPPRVSDIVAEAGSSNKAFYRYFAGKDDLILAVME 75 ++EA E + IL A+R+ + + +I AG + A Y +F K DL + E Sbjct: 5 TKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSEIWE 64 Query: 76 RGVAIVVSYLGHQMAKESRPDTKIARWIEGTLAQVADPHLISMSRAAAGQLSNWLATQRE 135 + + AK P ++ E + + R + + E Sbjct: 65 LSESNIGELELEYQAK--FPGDPLSVLREILIHVLESTVTEERRRLLMEIIFHKCEFVGE 122 Query: 136 M 136 M Sbjct: 123 M 123
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_4387	DHBDHDRGNASE	31	0.004	2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase signature. Length = 261 Score = 31.2 bits (70), Expect = 0.004 Identities = 16/64 (25%), Positives = 25/64 (39%), Gaps = 2/64 (3%) Query: 137 QGDWVVVLGAAGGVGLAAVDLAVAMGARVLAAASSPEKLGLCRQRGAEAVVDYDQEDLKL 196 +G + GAA G+G A + GA + A +PEKL + E Sbjct: 7 EGKIAFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVS--SLKAEARHAEAFPA 64 Query: 197 RIRE 200 +R+ Sbjct: 65 DVRD 68
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_4391	HTHFIS	104	3e-28	FIS bacterial regulatory protein HTH signature.
>HTHFIS#FIS bacterial regulatory protein HTH signature. Length = 484 Score = 104 bits (262), Expect = 3e-28 Identities = 35/118 (29%), Positives = 65/118 (55%) Query: 10 SVLVVDDEPVLADMVSMALRYEGWNIATASDGASAIASARAERPDVVVLDVMLPDMSGLE 69 ++LV DD+ + +++ AL G+++ S+ A+ A D+VV DV++PD + + Sbjct: 5 TILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAFD 64 Query: 70 VLHRLRKENPRLPVLLLTAKDAVEDRIAGLTAGGDDYVTKPFSIEEIVLRLRALLRRT 127 +L R++K P LPVL+++A++ I G DY+ KPF + E++ + L Sbjct: 65 LLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAEP 122
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_4399	cloacin	38	1e-04	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 37.8 bits (87), Expect = 1e-04 Identities = 33/93 (35%), Positives = 41/93 (44%), Gaps = 4/93 (4%) Query: 276 AGAGGQGGLGGDGNYAGQGSYGGVGGAGGAGGAAGMFGSGGSGGAGGEGGAFGDGNAGGS 335 +G G+G G + +G + GG G G GGA+ G G G G GGS Sbjct: 2 SGGDGRGHNTGAHSTSGNIN-GGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGS 60 Query: 336 GSGGHGGNGGNGGWLYGDAGAGGQGGNAAPGAY 368 G G GGNG +GG G G AAP A+ Sbjct: 61 GHGNGGGNGNSGG---GSGTGGNLSAVAAPVAF 90 Score = 36.2 bits (83), Expect = 2e-04 Identities = 35/104 (33%), Positives = 41/104 (39%), Gaps = 14/104 (13%) Query: 125 GAAGTVANPNGGAGGFLYGNGGN---GFSQTTAGVAGGAGGAAGLIGNGGMGGAGGAGAA 181 GA T N NGG G G G + G+S GG+G G G G GG G + Sbjct: 12 GAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNGNS 71 Query: 182 GGAGGTGGWWFGNGGVGGAGGAGTAGAVGFNGVSGGAGGAGGAA 225 GG GTGG + A V F + GAGG A Sbjct: 72 GGGSGTGG-----------NLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 34.7 bits (79), Expect = 8e-04 Identities = 27/80 (33%), Positives = 34/80 (42%), Gaps = 1/80 (1%) Query: 172 MGGAGGAGAAGGAGGTGGWWFGNGGVGGAGGAGTAGAVGFNGVSGGAGGAGGAAGWWGAG 231 M G G G GA T G G G GG + G+ G++ + GG G+ WG G Sbjct: 1 MSGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGS-GWSSENNPWGGGSGSGIHWGGG 59 Query: 232 GAGGQGGTGGMAGGYDNVNG 251 G GG G +GG G Sbjct: 60 SGHGNGGGNGNSGGGSGTGG 79 Score = 34.3 bits (78), Expect = 0.001 Identities = 32/110 (29%), Positives = 40/110 (36%), Gaps = 7/110 (6%) Query: 354 AGAGGQGGNAAPGAYSDNLGTFQSSYTSGNGGAGGNGGVAGMIGTGGAGGAGGAGGVNAF 413 +G G+G N + S N+ G G G GG + G G G + Sbjct: 2 SGGDGRGHNTGAHSTSGNI-------NGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGI 54 Query: 414 DSGGGGGGGGNGGNGGAGGASGALIEAGGVGGSGGGGGAGVVGPPAGNPG 463 GGG G G GGNG +GG SG V G + P AG Sbjct: 55 HWGGGSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 33.9 bits (77), Expect = 0.001 Identities = 32/110 (29%), Positives = 38/110 (34%), Gaps = 8/110 (7%) Query: 311 MFGSGGSGGAGGEGGAFGDGNAGGSGSGGHGGNGGNGGWLYGDAGAGGQGGNAAPGAYSD 370 M G G G G G+ N G +G G GG GW N G Sbjct: 1 MSGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGW--------SSENNPWGGGSGS 52 Query: 371 NLGTFQSSYTSGNGGAGGNGGVAGMIGTGGAGGAGGAGGVNAFDSGGGGG 420 + S GG G +GG +G G A A A G A + G GG Sbjct: 53 GIHWGGGSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG 102 Score = 33.9 bits (77), Expect = 0.001 Identities = 27/81 (33%), Positives = 30/81 (37%) Query: 395 MIGTGGAGGAGGAGGVNAFDSGGGGGGGGNGGNGGAGGASGALIEAGGVGGSGGGGGAGV 454 M G G G GA + +GG G G GG G S GG GSG G G Sbjct: 1 MSGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGS 60 Query: 455 VGPPAGNPGNDGLDGVTGASG 475 G GN G TG + Sbjct: 61 GHGNGGGNGNSGGGSGTGGNL 81 Score = 32.4 bits (73), Expect = 0.005 Identities = 40/129 (31%), Positives = 48/129 (37%), Gaps = 20/129 (15%) Query: 332 AGGSGSGGHGGNGGNGGWLYGDAGAGGQGGNAAPGAYSDNLGTFQSSYTSGNGGAGGNGG 391 +GG G G + G G + G G GG A + G+ SS + GG G+G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGA-------SDGSGWSSENNPWGGGSGSG- 53 Query: 392 VAGMIGTGGAGGAGGAGGVNAFDSGGGGGGGGNGGNGGAGGASGALIEAGGVGGSGGGGG 451 I GG G G GG G GG G GG A A + G S G G Sbjct: 54 ----IHWGGGSGHGN--------GGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAG 101 Query: 452 AGVVGPPAG 460 V AG Sbjct: 102 GLAVSISAG 110 Score = 31.6 bits (71), Expect = 0.007 Identities = 23/80 (28%), Positives = 30/80 (37%) Query: 259 NGGDGGAGGRGGWLFGDAGAGGQGGLGGDGNYAGQGSYGGVGGAGGAGGAAGMFGSGGSG 318 +GGDG G GG GLG G + + G G +G+ GGSG Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 319 GAGGEGGAFGDGNAGGSGSG 338 G G G +G G+ Sbjct: 62 HGNGGGNGNSGGGSGTGGNL 81 Score = 31.6 bits (71), Expect = 0.007 Identities = 32/106 (30%), Positives = 36/106 (33%), Gaps = 9/106 (8%) Query: 258 GNGGDGGAGGRGGWLFGDAGAGGQGGLGGDG---------NYAGQGSYGGVGGAGGAGGA 308 G G + GA G + G G GG DG G GS GG G G Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNG 65 Query: 309 AGMFGSGGSGGAGGEGGAFGDGNAGGSGSGGHGGNGGNGGWLYGDA 354 G SGG G GG A A G + G GG + A Sbjct: 66 GGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGA 111 Score = 31.6 bits (71), Expect = 0.008 Identities = 31/103 (30%), Positives = 40/103 (38%), Gaps = 6/103 (5%) Query: 143 GNGGNGFSQTTAGVAGGAGGAAGLIGNG--GMGGAGGAGAAGGAGGTGGWWFGNGGVGGA 200 G G N + +T+G G G+ G G G + GG G+G W G G G Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNG 65 Query: 201 GGAGTAGAVGFNGVSGGAGGAGGAAGWWGAGGAGGQGGTGGMA 243 GG G +G G G + AA A G GG+A Sbjct: 66 GGNGNSG----GGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 30.5 bits (68), Expect = 0.018 Identities = 26/94 (27%), Positives = 31/94 (32%), Gaps = 3/94 (3%) Query: 153 TAGVAGGAGGAAGLIGNGGMGGAGGAGAAGGAGGTGGWWFGNGGVGGAGGAGTAGAVGFN 212 + G G A GG G G GGA GW N GG G+G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIH---WGG 58 Query: 213 GVSGGAGGAGGAAGWWGAGGAGGQGGTGGMAGGY 246 G G GG G +G G +A G+ Sbjct: 59 GSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGF 92 Score = 29.7 bits (66), Expect = 0.032 Identities = 38/129 (29%), Positives = 47/129 (36%), Gaps = 14/129 (10%) Query: 309 AGMFGSGGSGGAGGEGGAFGDGNAGGSGSGGHGGNGGNGGWLYGDAGAGGQGGNAAPGAY 368 +G G G + GA G G G G G + G+G + GG G G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGG--GASDGSGWSSENNPWGGGSGSGIHWGG- 58 Query: 369 SDNLGTFQSSYTSGNGGAGGNGGVAGMIGTGGAGGAGGAGGVNAFDSGGGGGGGGNGGNG 428 SG+G GGNG G GTGG A A F + G GG + Sbjct: 59 -----------GSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSI 107 Query: 429 GAGGASGAL 437 AG S A+ Sbjct: 108 SAGALSAAI 116

MUL_4444

MUL_4455

Genomic Island

LocusTag	DNBias	CDNBias	%GCBias	Product
MUL_4444	3	11	0.337869	PE family protein
MUL_4445	3	13	1.130914	hypothetical protein
MUL_4446	1	15	0.867489	short-chain type dehydrogenase/reductase
MUL_4447	1	17	-0.042104	hydrolase
MUL_4449	3	26	-1.652880	hypothetical protein
MUL_4450	1	25	-2.325320	hypothetical protein
MUL_4451	0	30	-3.794000	hypothetical protein
MUL_4453	0	31	-4.425403	hypothetical protein
MUL_4454	-1	30	-4.541691	PE-PGRS family protein
MUL_4455	-1	24	-3.791570	non-IS element not present in Mycobacterium

MUL_4533

MUL_4548

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
MUL_4533	2	14	-2.518041	hypothetical protein
MUL_4534	2	14	-3.284759	periplasmic phosphate-binding lipoprotein PstS2
MUL_4535	2	16	-3.512909	phosphate-transport integral membrane ABC
MUL_4536	1	15	-2.665890	phosphate-transport integral membrane ABC
MUL_4537	1	12	-3.779163	periplasmic phosphate-binding lipoprotein PhoS2
MUL_4538	3	12	-3.063467	short chain dehydrogenase
MUL_4539	2	14	-1.903815	hypothetical protein
MUL_4540	1	15	-0.653314	manganese transport protein MntH
MUL_4541	1	14	0.409836	hypothetical protein
MUL_4542	3	16	-0.372343	hypothetical protein
MUL_4543	1	12	0.482501	*hypothetical protein
MUL_4544	0	11	-0.229671	hypothetical protein
MUL_4545	0	10	0.641690	hypothetical protein
MUL_4546	0	8	0.067768	non-IS element not present in Mycobacterium
MUL_4547	1	8	-0.391264	hypothetical protein
MUL_4548	2	10	1.014168	transposase

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_4540	HTHTETR	49	3e-09	TetR bacterial regulatory protein HTH signature.
>HTHTETR#TetR bacterial regulatory protein HTH signature. Length = 215 Score = 48.9 bits (116), Expect = 3e-09 Identities = 34/183 (18%), Positives = 64/183 (34%), Gaps = 16/183 (8%) Query: 10 RRWHQHKVERRNELVDGTIVAIRRHGRF-LSMDEIAAEIGVSKTVLYRYFVDKNDLTTAV 68 R+ Q E R ++D + + G S+ EIA GV++ +Y +F DK+DL + + Sbjct: 3 RKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSEI 62 Query: 69 M---MRFAQTTLIPNMAAALSSNLDGFDLAREIIRVYVETVAAEPEPYRFVMANSSASKS 125 + A L REI+ +E+ E + + Sbjct: 63 WELSESNIGELELEYQAKFPGDPLSVL---REILIHVLESTVTEERRRLLMEIIFHKCEF 119 Query: 126 ----KVIADSERIIA---RMLAVMLRRRVAEAGMDTGGVEP--WAYLIVGGVQLATHSWM 176 V+ ++R + + EA M + A ++ G + +W+ Sbjct: 120 VGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMTRRAAIIMRGYISGLMENWL 179 Query: 177 SDP 179 P Sbjct: 180 FAP 182

MUL_4571

MUL_4590

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
MUL_4571	1	13	-3.472054	hypothetical protein
MUL_4572	1	9	-2.552578	hypothetical protein
MUL_4573	0	8	-2.214143	oxidoreductase
MUL_4574	-1	10	-2.232218	transcriptional regulatory protein
MUL_4575	-1	9	-1.848731	PE-PGRS family protein
MUL_4576	1	11	2.147306	hypothetical protein
MUL_4577	1	13	1.676431	PPE family protein
MUL_4578	2	13	0.761994	integral membrane acyltransferase
MUL_4579	2	16	0.248278	lipoprotein LprE
MUL_4580	2	17	-0.327937	hypothetical protein
MUL_4581	2	22	-0.639108	C-term transposase for IS2404
MUL_4582	0	23	-4.138668	N-term transposase for IS2404
MUL_4584	0	21	-4.080777	hypothetical protein
MUL_4585	1	21	-3.945167	alpha-ketoglutarate decarboxylase
MUL_4586	0	20	-4.285347	short-chain type dehydrogenase/reductase
MUL_4587	2	20	-3.850365	lipoprotein LpqZ
MUL_4589	2	20	-3.948404	[NAD] dependent malate oxidoreductase Mez
MUL_4590	3	19	-3.551728	malate dehydrogenase

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_4573	NUCEPIMERASE	133	3e-38	Nucleotide sugar epimerase signature.
>NUCEPIMERASE#Nucleotide sugar epimerase signature. Length = 334 Score = 133 bits (337), Expect = 3e-38 Identities = 33/151 (21%), Positives = 63/151 (41%), Gaps = 15/151 (9%) Query: 27 VLVTGACRFLGGYLTARLAQNPLISSVIAVDAIAPSKDMLRRMGRAE--------FVRAD 78 LVTGA F+G +++ RL + V+ +D + D+ + R E F + D Sbjct: 3 YLVTGAAGFIGFHVSKRLLEAG--HQVVGIDNLNDYYDVSLKQARLELLAQPGFQFHKID 60 Query: 79 IRN-PFIAKVIRNGDVDTVVHAAAASYAPRS-GGSAALKELNVMGAMQLFAACQKAPSVR 136 + + + + +G + V + S A + N+ G + + C+ ++ Sbjct: 61 LADREGMTDLFASGHFERVFISPHRLAVRYSLENPHAYADSNLTGFLNILEGCRHN-KIQ 119 Query: 137 RVVLKSTSEVYGSSPHDPVVFTEDSSSRRPF 167 ++ S+S VYG + P F+ D S P Sbjct: 120 HLLYASSSSVYGLNRKMP--FSTDDSVDHPV 148
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_4581	cloacin	39	3e-05	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 39.3 bits (91), Expect = 3e-05 Identities = 30/93 (32%), Positives = 36/93 (38%), Gaps = 3/93 (3%) Query: 435 AGGDGGAGGAGGAGANGGLLIGHGGAGGGGTGGNGHGRPIGSGGAGGDGGDGGTGGWLYG 494 +GGDG G +G + +GG G G GG S GG G+G G Sbjct: 2 SGGDGRGHNTGAHSTSGNI---NGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGG 58 Query: 495 NGGHGGTGATGGSGRHSGASGDGGDGGDAQAIG 527 GHG G G SG SG G+ A G Sbjct: 59 GSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFG 91 Score = 37.8 bits (87), Expect = 1e-04 Identities = 37/105 (35%), Positives = 42/105 (40%), Gaps = 5/105 (4%) Query: 272 GVGGSGGSGGATGLLGNSGAGGTGGQGGGGGRGFDGSLGAGGAEGTGAVGGSGGWLVGDG 331 G G G + GA GN G TG GGG S G+G + GG G + G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGA-----SDGSGWSSENNPWGGGSGSGIHWG 57 Query: 332 GTGGDGGQGGGGHLGGAFAGGGGDGGAGAVGGTGGWLLGDGGAGG 376 G G G GG G+ GG GG A G L GAGG Sbjct: 58 GGSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG 102 Score = 36.6 bits (84), Expect = 3e-04 Identities = 28/79 (35%), Positives = 36/79 (45%) Query: 411 GNGGAGGDGGAGGEGGDSSGGAASAGGDGGAGGAGGAGANGGLLIGHGGAGGGGTGGNGH 470 G G G + GA G+ +GG G GGA G + G G+G GG+GH Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 471 GRPIGSGGAGGDGGDGGTG 489 G G+G +GG G GG Sbjct: 63 GNGGGNGNSGGGSGTGGNL 81 Score = 35.1 bits (80), Expect = 7e-04 Identities = 29/93 (31%), Positives = 36/93 (38%), Gaps = 1/93 (1%) Query: 450 NGGLLIGHGGAGGGGTGGNGHGRPIGSGGAGGDGGDGGTGGWLYGNGGHGGTGATGGSGR 509 +GG GH G T GN +G P G G GG G GG G+G G G Sbjct: 2 SGGDGRGHNT-GAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGS 60 Query: 510 HSGASGDGGDGGDAQAIGDGGAGGSGGLLFGAP 542 G G G+ G G + + + FG P Sbjct: 61 GHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFP 93 Score = 34.7 bits (79), Expect = 0.001 Identities = 31/95 (32%), Positives = 37/95 (38%) Query: 143 NGGAGGTGYSPTTGSGAVGGDGGAGGTGGWLYGSGGSGGIGGVGGSGTIGAPSGHGGSCG 202 N GA T + G +G GGA GW + GG G G G+ G+GG G Sbjct: 10 NTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNG 69 Query: 203 LGGGTGLFGQGGAGGNGGQGGGENFASTAGAGGPA 237 GG G + G ST GAGG A Sbjct: 70 NSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 34.3 bits (78), Expect = 0.001 Identities = 33/112 (29%), Positives = 42/112 (37%), Gaps = 10/112 (8%) Query: 337 GGQGGGGHLGGAFAGGGGDGGAGAVGGTGGWLLGDGGAGGDGGDGGDGGAASGFAGDGGQ 396 GG G G + G G +GG +G GG G G + + GG G+ + G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 397 GGQGAVGGASGWLLGNGGAGGDGGAGGEGGDSSGGAASAGGDGGAGGAGGAG 448 G G GNG +GG G GG + A GAGG Sbjct: 63 GNGG----------GNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 33.5 bits (76), Expect = 0.002 Identities = 39/122 (31%), Positives = 43/122 (35%), Gaps = 5/122 (4%) Query: 230 TAGAGGPAGTGGHGGWLYGNGGAGGIGGAGGVVGSSEGGVDGGVGGSGGSGGATGLLGNS 289 + G G TG H NGG G+G GG S + G GGSG G S Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWG-GGSGSGIHWGGGS 60 Query: 290 GAGGTGGQGGGGGRGFDGSLGAGGAEGTGAVGGSGGWLVGDGGTGGDGGQGGGGHLGGAF 349 G G GG G GG GS G A G + G GG G L A Sbjct: 61 GHGNGGGNGNSGG----GSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAAI 116 Query: 350 AG 351 A Sbjct: 117 AD 118 Score = 33.1 bits (75), Expect = 0.003 Identities = 30/89 (33%), Positives = 33/89 (37%), Gaps = 10/89 (11%) Query: 381 GGDGGAASGFAGDGGQGGQGAVGGASGWLLGNGGAGGDGGAGGEGGDSSGGAASAGGDGG 440 GGDG + G G + G L GGA G E GG+ S GG Sbjct: 3 GGDGRGHNT----GAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGG 58 Query: 441 AGGAGGAGANGGLLIGHGGAGGGGTGGNG 469 G G G NG GG GTGGN Sbjct: 59 GSGHGNGGGNG------NSGGGSGTGGNL 81 Score = 32.4 bits (73), Expect = 0.004 Identities = 33/111 (29%), Positives = 38/111 (34%), Gaps = 4/111 (3%) Query: 329 GDGGTGGDGGQGGGGHLGGAFAGGGGDGGAGAVGGTGG----WLLGDGGAGGDGGDGGDG 384 GDG G G++ G G G GGA G W G G GG G G Sbjct: 4 GDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHG 63 Query: 385 GAASGFAGDGGQGGQGAVGGASGWLLGNGGAGGDGGAGGEGGDSSGGAASA 435 GG G G + + + A GAGG S GA SA Sbjct: 64 NGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSA 114 Score = 30.5 bits (68), Expect = 0.020 Identities = 24/78 (30%), Positives = 29/78 (37%) Query: 289 SGAGGTGGQGGGGGRGFDGSLGAGGAEGTGAVGGSGGWLVGDGGTGGDGGQGGGGHLGGA 348 SG G G G + + G G G GW + GG G G G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 349 FAGGGGDGGAGAVGGTGG 366 GGG+G +G GTGG Sbjct: 62 HGNGGGNGNSGGGSGTGG 79 Score = 29.7 bits (66), Expect = 0.039 Identities = 23/83 (27%), Positives = 28/83 (33%) Query: 213 GGAGGNGGQGGGENFASTAGAGGPAGTGGHGGWLYGNGGAGGIGGAGGVVGSSEGGVDGG 272 GG G G GG + + + P G G G +G G G GG G G G Sbjct: 22 GGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNGNSGGGSGTGGNL 81 Query: 273 VGGSGGSGGATGLLGNSGAGGTG 295 + L GAGG Sbjct: 82 SAVAAPVAFGFPALSTPGAGGLA 104
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_4587	IGASERPTASE	31	0.011	IgA-specific serine endopeptidase (S6) signature.
>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature. Length = 1541 Score = 30.8 bits (69), Expect = 0.011 Identities = 17/51 (33%), Positives = 20/51 (39%), Gaps = 3/51 (5%) Query: 283 VTFDEDRHRTHTGNGAQVLATLRNTAINLHRLNGADNIAEACRITALTANR 333 V E+ H TGN L N I+ LN ADN + LT N Sbjct: 846 VRLTENSHWHLTGNSDVHQLDLANGHIH---LNSADNSNNVTKYNTLTVNS 893
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_4589	IGASERPTASE	30	0.021	IgA-specific serine endopeptidase (S6) signature.
>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature. Length = 1541 Score = 29.6 bits (66), Expect = 0.021 Identities = 17/51 (33%), Positives = 20/51 (39%), Gaps = 3/51 (5%) Query: 283 VTFDEDRHRAHTGNGAQVLATLRNTAINLHRLNGADNIAEACRITALTANR 333 V E+ H TGN L N I+ LN ADN + LT N Sbjct: 846 VRLTENSHWHLTGNSDVHQLDLANGHIH---LNSADNSNNVTKYNTLTVNS 893

MUL_4741

MUL_4755

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
MUL_4741	2	17	-2.244385	methionyl-tRNA synthetase
MUL_4742	2	20	-2.774657	non-IS element not present in Mycobacterium
MUL_4744	2	24	-3.250208	serine acetyltransferase CysE
MUL_4746	2	19	-2.518658	dehydrogenase
MUL_4747	2	15	-1.473252	aminodeoxychorismate synthase component I
MUL_4749	2	15	-1.286683	hypothetical protein
MUL_4751	1	14	-0.940112	hypothetical protein
MUL_4752	1	13	-0.995272	arginine deiminase
MUL_4753	1	11	-0.507197	hypothetical protein
MUL_4754	1	12	-0.319266	hypothetical protein
MUL_4755	2	13	-0.990556	hypothetical protein

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_4751	IGASERPTASE	30	0.002	IgA-specific serine endopeptidase (S6) signature.
>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature. Length = 1541 Score = 29.6 bits (66), Expect = 0.002 Identities = 17/51 (33%), Positives = 20/51 (39%), Gaps = 3/51 (5%) Query: 40 VTFDEDRHRAHTGNGAQVLATLRNTAINLHRLNGADNIAEACRITALTANR 90 V E+ H TGN L N I+ LN ADN + LT N Sbjct: 846 VRLTENSHWHLTGNSDVHQLDLANGHIH---LNSADNSNNVTKYNTLTVNS 893
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_4753	YERSSTKINASE	34	0.003	Yersinia serine/threonine protein kinase signature.
>YERSSTKINASE#Yersinia serine/threonine protein kinase signature. Length = 732 Score = 34.3 bits (78), Expect = 0.003 Identities = 30/78 (38%), Positives = 38/78 (48%), Gaps = 7/78 (8%) Query: 122 ILHRDIKPANVLLTDF-GEPALTDFGLAHMAGGFRTATGLFTASPAFTASELARGG-EPD 179 ++H DIKP NV+ GEP + D GL +G G FT S F A EL G Sbjct: 266 VVHNDIKPGNVVFDRASGEPVVIDLGLHSRSG--EQPKG-FTES--FKAPELGVGNLGAS 320 Query: 180 RASDVYGVGATLFCALTG 197 SDV+ V +TL + G Sbjct: 321 EKSDVFLVVSTLLHCIEG 338
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_4754	YERSSTKINASE	40	4e-05	Yersinia serine/threonine protein kinase signature.
>YERSSTKINASE#Yersinia serine/threonine protein kinase signature. Length = 732 Score = 40.5 bits (94), Expect = 4e-05 Identities = 52/199 (26%), Positives = 84/199 (42%), Gaps = 49/199 (24%) Query: 35 ELDRVVAVKVLTADLEQNRPRFEREQRAMARLTGHPNIVSVLQVGHTPGGYPYLVMPFCS 94 +++R +A L A+LE + ++ + HPN+ +V + V+P+ + Sbjct: 163 KIERSIAEGHLFAELEAYKHIYKTAGK-------HPNLANV---------HGMAVVPYGN 206 Query: 95 RGSVQEVITECGGLAVSEVLR-LGVAVAAGLES-----------AHRL----------GI 132 R ++ E G S+ LR L + G + AHRL G+ Sbjct: 207 RKEEALLMDEVDGWRCSDTLRTLADSWKQGKINSEAYWGTIKFIAHRLLDVTNHLAKAGV 266 Query: 133 VHRDVKPANVLLTEL-GDPAVTDFGIAHMVGGFHTASGVFSA--TPDFTAPEVLSGK-EP 188 VH D+KP NV+ G+P V D G+ H+ SG T F APE+ G Sbjct: 267 VHNDIKPGNVVFDRASGEPVVIDLGL-------HSRSGEQPKGFTESFKAPELGVGNLGA 319 Query: 189 DQASDVYGLGATLFCALTG 207 + SDV+ + +TL + G Sbjct: 320 SEKSDVFLVVSTLLHCIEG 338

MUL_4825

MUL_4837

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
MUL_4825	0	9	3.532348	transposase for IS2404
MUL_4826	0	9	3.656219	TetR family transcriptional regulator
MUL_4827	0	11	1.364926	hypothetical protein
MUL_4828	0	11	1.026513	NAD(P) transhydrogenasePntB
MUL_4829	0	13	0.273846	NAD(P) transhydrogenase (subunit alpha) PntAb
MUL_4830	0	11	-0.224799	NAD(P) transhydrogenase PntA
MUL_4831	2	14	-1.653393	hypothetical protein
MUL_4832	3	13	-0.055367	hypothetical protein
MUL_4833	0	12	-0.921581	phosphotyrosine protein phosphatase PtpB
MUL_4834	1	13	-1.055080	hypothetical protein
MUL_4835	2	10	-0.503577	hypothetical protein
MUL_4837	2	11	-0.258038	beta-1,3-glucanase

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_4828	DHBDHDRGNASE	87	3e-22	2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase signature. Length = 261 Score = 87.0 bits (215), Expect = 3e-22 Identities = 62/193 (32%), Positives = 94/193 (48%), Gaps = 3/193 (1%) Query: 34 AVTGKTALVTGASYGIGEATARKLAAAGATVLMVARSAERLEDLVSAIAAGGGAAVAYPT 93 + GK A +TGA+ GIGEA AR LA+ GA + V + E+LE +VS++ A A A+P Sbjct: 5 GIEGKIAFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHAEAFPA 64 Query: 94 DLTDEAAVGVLTKQVNQNHGPLDIVVSNAGKSLRRSLHDQYDRPHDFQRTIDINYLGPIW 153 D+ D AA+ +T ++ + GP+DI+V+ AG LR L +++ T +N G Sbjct: 65 DVRDSAAIDEITARIEREMGPIDILVNVAG-VLRPGLIHSLSD-EEWEATFSVNSTGVFN 122 Query: 154 LLLGLLPAMRESGRGHVVNVSSVGVRLVPGPQWGAYQASKGAFDRWLRSVAPELHGDGVD 213 + M + G +V V S VP AY +SK A + + + EL + Sbjct: 123 ASRSVSKYMMDRRSGSIVTVGSNPAG-VPRTSMAAYASSKAAAVMFTKCLGLELAEYNIR 181 Query: 214 VTSVYFALVRTRM 226 V T M Sbjct: 182 CNIVSPGSTETDM 194
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_4837	HTHTETR	55	1e-11	TetR bacterial regulatory protein HTH signature.
>HTHTETR#TetR bacterial regulatory protein HTH signature. Length = 215 Score = 55.0 bits (132), Expect = 1e-11 Identities = 32/179 (17%), Positives = 55/179 (30%), Gaps = 14/179 (7%) Query: 18 RTILDTAHAVFETYGVRRANIEDVATRAGVSRSTIYRRFPTKDELFERVVRREAELFFAA 77 + ILD A +F GV ++ ++A AGV+R IY F K +LF + Sbjct: 14 QHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSEIWELSESNIGEL 73 Query: 78 LNQ------ATNGHNPREAVIEAFTL-----GVRLIRDSPLYSRIAESEPELFGMFSRSH 126 + RE +I RL+ + + E + R+ Sbjct: 74 ELEYQAKFPGDPLSVLREILIHVLESTVTEERRRLLMEIIFHKCEFVGEMAVVQQAQRNL 133 Query: 127 VFPIGQFADGIAHTLRRCGADAPDADLANIADILLRVAVGII---MFPTERLDTSDDAA 182 + D A I+ G++ +F + D +A Sbjct: 134 CLESYDRIEQTLKHCIEAKMLPADLMTRRAAIIMRGYISGLMENWLFAPQSFDLKKEAR 192

MUL_0124

MUL_0127

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
MUL_0124	0	11	0.914922	orotate phosphoribosyltransferase
MUL_0125	0	10	0.235452	ketoacyl reductase
MUL_0126	-1	10	-0.615700	hypothetical protein
MUL_0127	-2	11	-1.364755	enoyl-CoA hydratase, EchA8

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_0124	DHBDHDRGNASE	67	2e-15	2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase signature. Length = 261 Score = 67.4 bits (164), Expect = 2e-15 Identities = 52/184 (28%), Positives = 87/184 (47%), Gaps = 4/184 (2%) Query: 5 VALITGPTSGIGAGYARRYAQDGYDLILVARDVDRLTQSAVELEDDAGNVEILPADLADA 64 +A ITG GIG AR A G + V + ++L + L+ +A + E PAD+ D+ Sbjct: 10 IAFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHAEAFPADVRDS 69 Query: 65 AGRDKVAERLSR---GVRVLVNNAGFATSGEFWETEPAALQAQLDVNVTAVMQLTRAALP 121 A D++ R+ R + +LVN AG G +A VN T V +R+ Sbjct: 70 AAIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGVFNASRSVSK 129 Query: 122 PMLAAGAGTVINIAS-VAGLLSGRGSTYSASKAWVISFSEGLSTGLEGTGVGVHAVCPGY 180 M+ +G+++ + S AG+ + Y++SKA + F++ L L + + V PG Sbjct: 130 YMMDRRSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLELAEYNIRCNIVSPGS 189 Query: 181 VHTE 184 T+ Sbjct: 190 TETD 193
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_0125	PF05616	30	0.007	Neisseria meningitidis TspB protein
>PF05616#Neisseria meningitidis TspB protein Length = 501 Score = 30.5 bits (68), Expect = 0.007 Identities = 19/68 (27%), Positives = 27/68 (39%), Gaps = 1/68 (1%) Query: 201 PLPQESPQEAEESEPAQSGNRSLTPSRRPELPPRRAQVDPAAGLLPDASRRTPEPMRREE 260 PLP+ SP E + PA + N P+ P+ P +P P +P R Sbjct: 327 PLPEVSPAENPANNPAPNENPGTRPNPEPD-PDLNPDANPDTDGQPGTRPDSPAVPDRPN 385 Query: 261 GRSEGSRR 268 GR R+ Sbjct: 386 GRHRKERK 393
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_0126	STREPKINASE	32	0.003	Streptococcus streptokinase protein signature.
>STREPKINASE#Streptococcus streptokinase protein signature. Length = 440 Score = 31.6 bits (71), Expect = 0.003 Identities = 22/69 (31%), Positives = 33/69 (47%), Gaps = 9/69 (13%) Query: 187 LPGEELWRFVDKLAGRIASYPEEAIAAAKRAVDV-------ALDPRTDLTTGLRIEDQLL 239 LP + + F+ R+ Y E+ I ++VDV L+P D GL+ D L Sbjct: 153 LPTQPVQEFLLSGHVRVRPYKEKPIQNQAKSVDVEYTVQFTPLNPDDDFRPGLK--DTKL 210 Query: 240 RETLALPDT 248 +TLA+ DT Sbjct: 211 LKTLAIGDT 219
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_0127	HTHFIS	43	6e-06	FIS bacterial regulatory protein HTH signature.
>HTHFIS#FIS bacterial regulatory protein HTH signature. Length = 484 Score = 42.5 bits (100), Expect = 6e-06 Identities = 39/184 (21%), Positives = 66/184 (35%), Gaps = 30/184 (16%) Query: 550 AGRMLEGETAKLLRMEDEL--GHRVIGQKKAVQAVSDAVRRSRAGVADPNRPTGSFMFLG 607 GR L + ++ED+ G ++G+ A+Q + + R + + M G Sbjct: 115 IGRALAEPKRRPSKLEDDSQDGMPLVGRSAAMQEIYRVLAR----LMQTDLTL---MITG 167 Query: 608 PTGVGKTELAKALAEFLFDDERAMVRIDMSEYGEKHSVARLVGAPPGYIGYDHGGQLTEA 667 +G GK +A+AL ++ V I+M+ + L G G T A Sbjct: 168 ESGTGKELVARALHDYGKRRNGPFVAINMAAIPRDLIESELFGHEKG--------AFTGA 219 Query: 668 VRRRPYTV-------ILFDEIEKAHPDVFDVLLQVLDEG---RLTDGQGRTVDFRNTILI 717 R + DEI D LL+VL +G + D R ++ Sbjct: 220 QTRSTGRFEQAEGGTLFLDEIGDMPMDAQTRLLRVLQQGEYTTVGGRTPIRSDVR---IV 276 Query: 718 LTSN 721 +N Sbjct: 277 AATN 280 Score = 29.8 bits (67), Expect = 0.049 Identities = 14/60 (23%), Positives = 30/60 (50%), Gaps = 3/60 (5%) Query: 159 QALEKYSTDLTARAREG-KLDPVIGRDNEIRRVVQVLSRRTKNN-PVLI-GEPGVGKTAI 215 +AL + + + P++GR ++ + +VL+R + + ++I GE G GK + Sbjct: 117 RALAEPKRRPSKLEDDSQDGMPLVGRSAAMQEIYRVLARLMQTDLTLMITGESGTGKELV 176

MUL_0135

MUL_0141

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
MUL_0135	0	9	-1.832377	hypothetical protein
MUL_0136	-1	7	-0.684689	TetR family transcriptional regulator
MUL_0137	-1	8	-0.762933	short-chain type dehydrogenase/reductase
MUL_0138	-1	8	-0.381670	hypothetical protein
MUL_0139	-1	9	-0.813762	5-
MUL_0140	-2	10	0.071598	hypothetical protein
MUL_0141	-1	9	0.352621	hypothetical protein

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_0135	HTHTETR	63	1e-13	TetR bacterial regulatory protein HTH signature.
>HTHTETR#TetR bacterial regulatory protein HTH signature. Length = 215 Score = 63.1 bits (153), Expect = 1e-13 Identities = 31/156 (19%), Positives = 57/156 (36%), Gaps = 2/156 (1%) Query: 16 RRTEILQTAAALIASSGLR-TSLQEIADAAGILPGSLYHHFESKEAILIELIRRYQDDLH 74 R IL A L + G+ TSL EIA AAG+ G++Y HF+ K + E+ + ++ Sbjct: 12 TRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSEIWELSESNIG 71 Query: 75 Q-IGQSWQAKLDQPDSRTVAEKITQLGAAIANCAVAHRAALQMSFYEGPSADPELMKLTS 133 + + P S I L + + + E + + Sbjct: 72 ELELEYQAKFPGDPLSVLREILIHVLESTVTEERRRLLMEIIFHKCEFVGEMAVVQQAQR 131 Query: 134 QRPLAIQEAMLQTLRAGRWSGYIRTEIDLPTFADRI 169 L + + QTL+ + + ++ A + Sbjct: 132 NLCLESYDRIEQTLKHCIEAKMLPADLMTRRAAIIM 167 Score = 43.8 bits (103), Expect = 3e-07 Identities = 19/83 (22%), Positives = 33/83 (39%), Gaps = 1/83 (1%) Query: 237 SDKAAHVRAVARMEFGRKGYEVTTVRDIASASGLGTGTVYRVIGSKDKLLASIM-RSFGQ 295 + H+ VA F ++G T++ +IA A+G+ G +Y K L + I S Sbjct: 10 QETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSEIWELSESN 69 Query: 296 KVEAGWVAVRRSNATPIEKLDAL 318 E + P+ L + Sbjct: 70 IGELELEYQAKFPGDPLSVLREI 92
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_0136	DHBDHDRGNASE	59	4e-12	2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase signature. Length = 261 Score = 58.5 bits (141), Expect = 4e-12 Identities = 67/277 (24%), Positives = 103/277 (37%), Gaps = 54/277 (19%) Query: 11 DGKRALIVGGATGMGAAAAKSAAELGAEVIVMDYAPVGYDA-----------AQTLSVDL 59 +GK A I G A G+G A A++ A GA + +DY P + A+ D+ Sbjct: 7 EGKIAFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHAEAFPADV 66 Query: 60 RDPASIDSAVERLG---GPVHAVFSAAGVADGPDLMKINFIGHRHLIDRLLANDQLPSGS 116 RD A+ID R+ GP+ + + AGV R L Sbjct: 67 RDSAAIDEITARIEREMGPIDILVNVAGVL------------------RPGLIHSLSD-E 107 Query: 117 AVCFISSVAGMGWENDLPRLTEFLATPDYGAAQDWVS--AHEAE-GIIHYGFSKKAINAY 173 SV G N +++++ G+ S A + Y SK A + Sbjct: 108 EWEATFSVNSTGVFNASRSVSKYMMDRRSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMF 167 Query: 174 VATRAYPLLKRGIRINAICPGPTDTPLAQANADLWLT----------FAQDYRDETG--- 220 L + IR N + PG T+T + + LW + ++ TG Sbjct: 168 TKCLGLELAEYNIRCNIVSPGSTETDMQWS---LWADENGAEQVIKGSLETFK--TGIPL 222 Query: 221 SKVHTPEQMGDVMVFLNSAAAFGISGITLLVDYGHTM 257 K+ P + D ++FL S A I+ L VD G T+ Sbjct: 223 KKLAKPSDIADAVLFLVSGQAGHITMHNLCVDGGATL 259
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_0137	NUCEPIMERASE	35	1e-04	Nucleotide sugar epimerase signature.
>NUCEPIMERASE#Nucleotide sugar epimerase signature. Length = 334 Score = 35.1 bits (81), Expect = 1e-04 Identities = 34/184 (18%), Positives = 57/184 (30%), Gaps = 35/184 (19%) Query: 4 VVVFGGHGKVALLLGHILADRGDQVSSV-----FRNP---DHRDDIAAT-GATPVQADIE 54 +V G G + + L + G QV + + + R ++ A G + D+ Sbjct: 3 YLVTGAAGFIGFHVSKRLLEAGHQVVGIDNLNDYYDVSLKQARLELLAQPGFQFHKIDLA 62 Query: 55 GLDTAALAGLLTGH--DAVVFSAGAGG-----GNPARTYAVDRDAATRVIDAATRAGVQR 107 D + L + V S NP + +++ +Q Sbjct: 63 --DREGMTDLFASGHFERVFISPHRLAVRYSLENPHAYADSNLTGFLNILEGCRHNKIQH 120 Query: 108 FVMIS---YFGAGPNHGVSVDDS----FFPYAQAKAAAD--AHLRASNLD--------WT 150 + S +G S DDS YA K A + AH + +T Sbjct: 121 LLYASSSSVYGLNRKMPFSTDDSVDHPVSLYAATKKANELMAHTYSHLYGLPATGLRFFT 180 Query: 151 VLGP 154 V GP Sbjct: 181 VYGP 184
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_0141	PHPHTRNFRASE	76	2e-16	Phosphoenolpyruvate-protein phosphotransferase sign...
>PHPHTRNFRASE#Phosphoenolpyruvate-protein phosphotransferase signature. Length = 572 Score = 75.6 bits (186), Expect = 2e-16 Identities = 29/99 (29%), Positives = 46/99 (46%), Gaps = 5/99 (5%) Query: 379 LAYTDVDEALDAADRGEQVILVRDHTRPEDVSGMLA--AQGIVTEIGGAASHAAVVSREL 436 L + E A E+ +++ + P D + + +G T+IGG SH+A++SR L Sbjct: 139 LGHLIGVETGSLATIAEETVIIAEDLTPSDTAQLNKQFVKGFATDIGGRTSHSAIMSRSL 198 Query: 437 GRVAVVGCGDGVAASLAGKRITVDGYTGEVREGILAPSA 475 AVVG + G + VDG G V I+ P+ Sbjct: 199 EIPAVVGTKEVTEKIQHGDMVIVDGIEGIV---IVNPTE 234

MUL_0237

MUL_0255

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
MUL_0237	-1	17	-0.805302	metal cation transporter ATPase p-type CtpE
MUL_0240	-1	18	-1.886963	hypothetical protein
MUL_0241	-2	19	-1.278029	enoyl-CoA hydratase
MUL_0242	-1	17	0.247184	acetyl-coenzyme a carboxylase carboxyl
MUL_0245	0	11	-0.092739	hypothetical protein
MUL_0247	1	10	-0.085358	8-amino-7-oxononanoate synthase
MUL_0248	-1	8	0.616791	cysteine synthase B
MUL_0249	-1	11	0.298463	transcriptional regulatory protein
MUL_0251	-2	9	-0.783869	hypothetical protein
MUL_0252	-2	10	-0.728593	two component response transcriptional
MUL_0253	-1	12	0.054950	two component sensor histidine kinase PrrB
MUL_0254	-2	9	-0.586105	hypothetical protein
MUL_0255	-2	11	-1.528218	outer membrane protein OmpA

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_0237	ARGDEIMINASE	30	0.019	Bacterial arginine deiminase signature.
>ARGDEIMINASE#Bacterial arginine deiminase signature. Length = 409 Score = 30.2 bits (68), Expect = 0.019 Identities = 10/35 (28%), Positives = 16/35 (45%), Gaps = 1/35 (2%) Query: 149 GVFASWGSLGHVTVAEPGALIGFLGP-RVYELLYD 182 +F+ G L V + PG + L P + L+D Sbjct: 9 NIFSEIGRLKKVLLHRPGEELENLTPFIMKNFLFD 43
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_0240	TCRTETB	89	4e-21	Tetracycline resistance protein TetB signature.
>TCRTETB#Tetracycline resistance protein TetB signature. Length = 458 Score = 89.2 bits (221), Expect = 4e-21 Identities = 82/408 (20%), Positives = 152/408 (37%), Gaps = 33/408 (8%) Query: 26 FIVYLDTTVLLVAFGAISASFPEASSSARSWVLDAYFIVFAALMVPGGRWADQFGSRNVF 85 F L+ VL V+ I+ F ++ +WV A+ + F+ G+ +DQ G + + Sbjct: 24 FFSVLNEMVLNVSLPDIANDF-NKPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLL 82 Query: 86 AIGVSTFILSSVGCAVAPTLGA-LVAARAAQAVGAALMGPASLALILPYFGRGSRATAVS 144 G+ SV V + + L+ AR Q GAA + ++ Y + +R A Sbjct: 83 LFGIIINCFGSVIGFVGHSFFSLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFG 142 Query: 145 LWGTSAALAAALGPPLGGFLADTVGWRGIFLINVPIGLAV-LAGLRNVDNRGDAVAGQLV 203 L G+ A+ +GP +GG +A + W +L+ +P+ + + L + + + G Sbjct: 143 LIGSIVAMGEGVGPAIGGMIAHYIHWS--YLLLIPMITIITVPFLMKLLKKEVRIKGHFD 200 Query: 204 NTSAIVLIASGVGALTAGILEGPSWGWGQRRTLLLLIAGAILLATAMVGVARHHRRAE-P 262 I++ + +L S+ I+ + + +H R+ P Sbjct: 201 IKGIILMSV----GIVFFMLFTTSYSISF----------LIVSVLSFLIFVKHIRKVTDP 246 Query: 263 IHDFD--KGRFFAANAATA--IFGAGFYGLLLAVVFFLTSHWHYSTFEAG-LAMMPIFVA 317 D K F IFG G + V + + ST E G + + P ++ Sbjct: 247 FVDPGLGKNIPFMIGVLCGGIIFGT-VAGFVSMVPYMMKDVHQLSTAEIGSVIIFPGTMS 305 Query: 318 AALAAIPAGRIADARGHRWAVIPGCWVFALGVFLFWLLTTSRADYASRWLP--GSILCGI 375 + G + D RG + + G ++ LT S + W + Sbjct: 306 VIIFGYIGGILVDRRGPLYVLNIGVTFLSVS-----FLTASFLLETTSWFMTIIIVFVLG 360 Query: 376 GIRCVMPVLASAAIDAMPGQLLGTANALNSMLRQFGAALGTAAVGTLL 423 G+ V+++ ++ Q G +L + G A VG LL Sbjct: 361 GLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTSFLSEGTGIAIVGGLL 408
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_0245	HTHTETR	46	1e-08	TetR bacterial regulatory protein HTH signature.
>HTHTETR#TetR bacterial regulatory protein HTH signature. Length = 215 Score = 46.2 bits (109), Expect = 1e-08 Identities = 31/159 (19%), Positives = 54/159 (33%), Gaps = 6/159 (3%) Query: 1 MFADKGFGHVRIEDVCAAAGYTRGAFYSQFDSLEELFFTLYDQRATLISEQVGTAMASV- 59 +F+ +G + ++ AAG TRGA Y F +LF +++ + I E A Sbjct: 23 LFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFP 82 Query: 60 GDPTDVPGTVDRIASTLLLDRDWLLIKTDFLMHAARHPDLAQRLAAHRAQLRAAVEDRLA 119 GDP V + + + + + + H + + L DR+ Sbjct: 83 GDPLSVLREILIHVLESTVTEERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIE 142 Query: 120 GSDVELPAAIGSVAD-----AARAVVAAYDGVSIQLLLD 153 + A AD AA + G+ L Sbjct: 143 QTLKHCIEAKMLPADLMTRRAAIIMRGYISGLMENWLFA 181
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_0248	HTHFIS	102	2e-27	FIS bacterial regulatory protein HTH signature.
>HTHFIS#FIS bacterial regulatory protein HTH signature. Length = 484 Score = 102 bits (257), Expect = 2e-27 Identities = 35/119 (29%), Positives = 58/119 (48%) Query: 11 PRVLVVDDDSDVLASLERGLRLSGFEVSTAVDGAEALRSATETRPDAIVLDINMPVLDGV 70 +LV DDD+ + L + L +G++V + A R D +V D+ MP + Sbjct: 4 ATILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAF 63 Query: 71 SVVTALRAMDNDVPVCVLSARSSVDDRVAGLEAGADDYLVKPFVLAELVARVKALLRRR 129 ++ ++ D+PV V+SA+++ + E GA DYL KPF L EL+ + L Sbjct: 64 DLLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAEP 122
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_0249	PF06580	36	3e-04	Sensor histidine kinase
>PF06580#Sensor histidine kinase Length = 349 Score = 35.6 bits (82), Expect = 3e-04 Identities = 19/101 (18%), Positives = 39/101 (38%), Gaps = 22/101 (21%) Query: 351 IANAVKHGGSTR-----VQLSAVSSRAGVEIAVDDNGSGVPEAERQMVFERFSRGSTASH 405 + N +KHG + + L V + V++ GS + ++ Sbjct: 264 VENGIKHGIAQLPQGGKILLKGTKDNGTVTLEVENTGSLALKNTKE-------------- 309 Query: 406 SGSGLGLALVAQQ-AHLHGGTASLQ-NSPLGGARLLLKLPG 444 +G GL V ++ L+G A ++ + G ++ +PG Sbjct: 310 -STGTGLQNVRERLQMLYGTEAQIKLSEKQGKVNAMVLIPG 349
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_0251	IGASERPTASE	30	8e-04	IgA-specific serine endopeptidase (S6) signature.
>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature. Length = 1541 Score = 29.6 bits (66), Expect = 8e-04 Identities = 15/57 (26%), Positives = 22/57 (38%), Gaps = 3/57 (5%) Query: 24 IVTVSIKPVATTAEAVEAEQTEQT-EQTEQTEQTE--QTEQAEEPDPAASHQTEVAQ 77 I V PV A A +E TE E ++Q +T + A E + A+ Sbjct: 1017 IARVDEAPVPPPAPATPSETTETVAENSKQESKTVEKNEQDATETTAQNREVAKEAK 1073
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_0252	OMPADOMAIN	104	1e-27	OMPA domain signature.
>OMPADOMAIN#OMPA domain signature. Length = 346 Score = 104 bits (260), Expect = 1e-27 Identities = 45/146 (30%), Positives = 68/146 (46%), Gaps = 16/146 (10%) Query: 197 GQPAGSTPPTGPAATGACADLQAAVTALTGGAIAFGNDGVSLTPDSNKVLTQVVDKLRAC 256 G+ A P A ++Q L + F + +L P+ L Q+ +L Sbjct: 194 GEAAPVVAPAPAPA----PEVQTKHFTLKS-DVLFNFNKATLKPEGQAALDQLYSQLSNL 248 Query: 257 --PDAKVTVNGYTDNSGSEGLNIPLSAQRAQTVADFLVAHGVATDHITAKGLGSANPIAS 314 D V V GYTD GS+ N LS +RAQ+V D+L++ G+ D I+A+G+G +NP+ Sbjct: 249 DPKDGSVVVLGYTDRIGSDAYNQGLSERRAQSVVDYLISKGIPADKISARGMGESNPVTG 308 Query: 315 NDTAEGR---------IKNRRVEIVV 331 N + +RRVEI V Sbjct: 309 NTCDNVKQRAALIDCLAPDRRVEIEV 334
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_0255	56KDTSANTIGN	30	0.029	Rickettsia 56kDa type-specific antigen protein sign...
>56KDTSANTIGN#Rickettsia 56kDa type-specific antigen protein signature. Length = 533 Score = 29.5 bits (66), Expect = 0.029 Identities = 13/35 (37%), Positives = 19/35 (54%) Query: 97 LPNTDQLAQFTGRIQRHTMLHEDLKRFFDGFPRNA 131 LPN+ + Q +IQ E+L+ FDG+ NA Sbjct: 291 LPNSASIEQIQSKIQELGDTLEELRDSFDGYINNA 325

MUL_0335

MUL_0344

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
MUL_0335	0	13	-0.382313	dipeptidase
MUL_0336	-1	12	-1.047503	cytochrome P450 105Q4 Cyp105Q4
MUL_0338	-1	12	-1.444741	ferredoxin
MUL_0340	0	10	-1.651706	hypothetical protein
MUL_0341	-1	9	-1.549167	short chain dehydrogenase
MUL_0342	1	10	-1.725956	integral membrane transport protein
MUL_0343	1	11	-1.329777	lipoprotein LpqS
MUL_0344	1	9	-0.086254	oxidase

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_0335	HTHTETR	52	3e-10	TetR bacterial regulatory protein HTH signature.
>HTHTETR#TetR bacterial regulatory protein HTH signature. Length = 215 Score = 51.6 bits (123), Expect = 3e-10 Identities = 26/108 (24%), Positives = 46/108 (42%), Gaps = 17/108 (15%) Query: 11 ERASSTQEAILVAAERLFAEHGVFAVSNRQVSEAAGQGNNAAVGYHFGTKTDLVRAI--- 67 + A T++ IL A RLF++ GV + S ++++AAG A+ +HF K+DL I Sbjct: 7 QEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGV-TRGAIYWHFKDKSDLFSEIWEL 65 Query: 68 -------------EQKHRVPIERLREQMVAAAAAKGAAATMRDWVACL 102 + P+ LRE ++ + R + + Sbjct: 66 SESNIGELELEYQAKFPGDPLSVLREILIHVLESTVTEERRRLLMEII 113
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_0336	DHBDHDRGNASE	112	4e-32	2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase signature. Length = 261 Score = 112 bits (282), Expect = 4e-32 Identities = 81/261 (31%), Positives = 124/261 (47%), Gaps = 11/261 (4%) Query: 5 LAGKIAIVTGGASGIGRATVARFIAEGARVVIADVEEERGESLAAALGADAMFC---RTD 61 + GKIA +TG A GIG A ++GA + D E+ E + ++L A+A D Sbjct: 6 IEGKIAFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHAEAFPAD 65 Query: 62 VSQPEQVAAVVAAAVDNFGGLHVMVNNAGV--SGAMHRRFLDDDLADFHRVMAVNVLGVM 119 V + + A G + ++VN AGV G +H ++ A F +VN GV Sbjct: 66 VRDSAAIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATF----SVNSTGVF 121 Query: 120 AGTRDAARHMAAHGGGSIVNLTSIGGIQAGGGVMTYRASKGAVIQFTKSAAIELAHYEIR 179 +R +++M GSIV + S + Y +SK A + FTK +ELA Y IR Sbjct: 122 NASRSVSKYMMDRRSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLELAEYNIR 181 Query: 180 VNAIAPGNIPTPLLASSAAGLDQEQVERFTAQIRQTMREDRPLKREGTPEDIAEAALYFA 239 N ++PG+ T + S A D+ E+ +T + PLK+ P DIA+A L+ Sbjct: 182 CNIVSPGSTETDMQWSLWA--DENGAEQVIKGSLETFKTGIPLKKLAKPSDIADAVLFLV 239 Query: 240 GERSRYVTGTVLPVDGGTVAG 260 ++ ++T L VDGG G Sbjct: 240 SGQAGHITMHNLCVDGGATLG 260
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_0338	TCRTETA	54	4e-10	Tetracycline resistance protein signature.
>TCRTETA#Tetracycline resistance protein signature. Length = 399 Score = 54.1 bits (130), Expect = 4e-10 Identities = 65/294 (22%), Positives = 109/294 (37%), Gaps = 9/294 (3%) Query: 11 NRPSRVLMINQFGINVGFYMLMPYLADYLA--GPLGLAAWAVGLVMGVRNFSQQGMFFVG 68 NRP V++ VG ++MP L L G+++ + Q V Sbjct: 4 NRPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHYGILLALYALMQFACAPVL 63 Query: 69 GTLADRFGYKPLIVAGCLIRTGGFALLVVAQSLPSVLIAAAATGFAGALFNPAVRAYVAA 128 G L+DRFG +P+++ +A++ A L + I G GA AV A Sbjct: 64 GALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIGRIVAGITGATG--AVAGAYIA 121 Query: 129 DS--GDRKLEAFATFNIFYQAGILLGPLVGLALLTLDFRMTVLGASAVFAVLTAAQLMAL 186 D GD + F + + G++ GP++G + A+A+ + L Sbjct: 122 DITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPFFAAAALNGLNFLTGCFLL 181 Query: 187 PQ-HLADPDTKNESILQGWKAIVCNRSFLGFAAAMTGAYVLSF--QVYLALPIQASLLAP 243 P+ H + L + R AA M +++ QV AL + Sbjct: 182 PESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIMQLVGQVPAALWVIFGEDRF 241 Query: 244 GHQSVLLAAMFAVSGLIAIAGQLRITRWVAAHWRVSRSLVVGAAILAMAFVPLA 297 + + A G++ Q IT VAA R+L++G ++ LA Sbjct: 242 HWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALMLGMIADGTGYILLA 295
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_0344	DHBDHDRGNASE	70	2e-16	2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase signature. Length = 261 Score = 70.5 bits (172), Expect = 2e-16 Identities = 72/279 (25%), Positives = 105/279 (37%), Gaps = 62/279 (22%) Query: 11 DGRRAVVTGCASGIGERAVHRLTGLGAQVVGLDQRQPGYEISE-------FHQ----IDL 59 +G+ A +TG A GIGE L GA + +D E H D+ Sbjct: 7 EGKIAFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHAEAFPADV 66 Query: 60 ADPGSIDRAVEAIG---GPVDALFNIAGV-SSGIGDPL------LVVTINFLGLRHLTEA 109 D +ID I GP+D L N+AGV G+ L ++N G+ + + + Sbjct: 67 RDSAAIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGVFNASRS 126 Query: 110 LLPMMGP--GSAIVSVSSLAAAGYREHHRAVAPLLNTTTMVEGIDWCKRHLEVLGAGYQL 167 + M +IV+V S A R T+M A Y Sbjct: 127 VSKYMMDRRSGSIVTVGSNPAGVPR------------TSM---------------AAYAS 159 Query: 168 SKEAVILYTMRSAVGLGARGIRINCTGPGVTETPILDQLTT----------AYGAEFPDD 217 SK A +++T + L IR N PG TET + L F Sbjct: 160 SKAAAVMFTKCLGLELAEYNIRCNIVSPGSTETDMQWSLWADENGAEQVIKGSLETFKTG 219 Query: 218 IAKPLGRVATPGEQASVLVFLNGHSASYISGQVVRVDGG 256 I PL ++A P + A ++FL A +I+ + VDGG Sbjct: 220 I--PLKKLAKPSDIADAVLFLVSGQAGHITMHNLCVDGG 256

MUL_0569

MUL_0593

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
MUL_0569	4	13	4.167258	hypothetical protein
MUL_0570	3	12	2.773269	hypothetical protein
MUL_0571	1	14	1.536556	deoxycytidine triphosphate deaminase
MUL_0572	1	12	2.085235	hypothetical protein
MUL_0573	3	12	0.959396	non-IS element not present in Mycobacterium
MUL_0574	3	12	0.794909	hypothetical protein
MUL_0575	1	15	0.594599	hypothetical protein
MUL_0576	1	13	1.712364	alpha-D-glucose-1-phosphate thymidylyl-
MUL_0578	2	14	2.283971	PE PGRS family protein
MUL_0579	2	14	2.562819	PE PGRS family protein
MUL_0580	0	11	1.105103	C-term transposase for IS2404
MUL_0581	1	13	2.054942	PE-PGRS family protein
MUL_0582	2	11	1.629389	non-IS element not present in Mycobacterium
MUL_0583	2	10	1.297982	non-IS element not present in Mycobacterium
MUL_0584	0	11	0.855137	aminotransferase AlaT
MUL_0585	0	11	0.490258	iron-sulfur-binding reductase
MUL_0587	1	11	0.754877	transposase for IS2606
MUL_0588	1	11	0.603221	C-term transposase for IS2404
MUL_0589	2	13	-0.291471	hypothetical protein
MUL_0593	1	13	-1.227130	membrane protein, IniB

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_0569	cloacin	40	8e-06	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 40.5 bits (94), Expect = 8e-06 Identities = 35/114 (30%), Positives = 46/114 (40%), Gaps = 5/114 (4%) Query: 230 GGNGGQGGTGGTLFGNGGGGGTGGAGFVGPSSAGDGGNGGGGGRAGLIGNGGAGGAGGAP 289 G N G T G + G G G GG G + + GGG +G+ GG+G Sbjct: 8 GHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGI---HWGGGSGHGN 64 Query: 290 GPNGGFSGGNGGNGGDAVLIGNGGNSGDVGLS--GAGTPGLPGNGGLLIDTIGN 341 G G SGG G GG+ + G LS GAG + + G L I + Sbjct: 65 GGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAAIAD 118 Score = 35.1 bits (80), Expect = 4e-04 Identities = 38/106 (35%), Positives = 44/106 (41%), Gaps = 11/106 (10%) Query: 201 GGDGRLFGNGGAGGVGGAAYTSNILMNATGGNGGQGGTGGTLFG-----NGGGGGTGGAG 255 GGDGR N GA G NI TG G G + G+ + GGG G+G Sbjct: 3 GGDGRGH-NTGAHSTSG-----NINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHW 56 Query: 256 FVGPSSAGDGGNGGGGGRAGLIGNGGAGGAGGAPGPNGGFSGGNGG 301 G GGNG GG +G GN A A A G + G GG Sbjct: 57 GGGSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG 102 Score = 29.7 bits (66), Expect = 0.021 Identities = 30/87 (34%), Positives = 38/87 (43%), Gaps = 8/87 (9%) Query: 147 GLEQQGGTGGAAGLFGNGGDGGATGIFGGTGGAGGAGGQSTNALADSVGGNGGQGGDGRL 206 G + +G GA GN +GG TG+ G G + G+G S N G+G G G Sbjct: 3 GGDGRGHNTGAHSTSGNI-NGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 207 FGNGGAGGVGGAAYTSNILMNATGGNG 233 GNGG G G + TGGN Sbjct: 62 HGNGGGNGNSGGG-------SGTGGNL 81 Score = 29.3 bits (65), Expect = 0.027 Identities = 36/119 (30%), Positives = 41/119 (34%), Gaps = 12/119 (10%) Query: 181 GAGGQSTNALADSVGGNGGQGGDGRLFGNGGAGGVGGAAYTSNILMNATGGNGGQGGTGG 240 G G+ N A S GN GG L GGA G + +N +G GG Sbjct: 3 GGDGRGHNTGAHSTSGNI-NGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGG--- 58 Query: 241 TLFGNGGGGGTGGAGFVGPSSAGDGGNGGGGGRAGLIGNG-GAGGAGGAPGPNGGFSGG 298 G G G GG G S G G G A + G A GA G S G Sbjct: 59 -----GSGHGNGGGN--GNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAG 110
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_0570	cloacin	37	1e-04	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 37.0 bits (85), Expect = 1e-04 Identities = 36/110 (32%), Positives = 46/110 (41%), Gaps = 2/110 (1%) Query: 223 GAGGDGGVGGFGTFAGNGGDGGTGL-FAAGGDSGAGGDSVGGADGGDGGNGGKAGLVGQG 281 G G G G + +GN G TGL G G+G S GG G+G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 282 GDGGAGGNNNSGFGTGGSGGKGGDAVLIGNGGNGGNAGSGGVGPGIAGAA 331 G+GG GN+ G GTGG+ V G G+GG+ I+ A Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPAL-STPGAGGLAVSISAGA 111 Score = 35.5 bits (81), Expect = 3e-04 Identities = 27/80 (33%), Positives = 30/80 (37%), Gaps = 5/80 (6%) Query: 194 GRGGAGGAGGFGNNTTGGIGGLGGAGGLFGAGGDGGVGGFGTFAGNGGDGGTGLFAAGGD 253 GRG GA N GG GLG GG G G GG G+G+ GG Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGG-----ASDGSGWSSENNPWGGGSGSGIHWGGGS 60 Query: 254 SGAGGDSVGGADGGDGGNGG 273 G G + GG G G Sbjct: 61 GHGNGGGNGNSGGGSGTGGN 80 Score = 34.3 bits (78), Expect = 7e-04 Identities = 33/112 (29%), Positives = 41/112 (36%), Gaps = 13/112 (11%) Query: 243 GGTGLFAAGGDSGAGGDSVGGADGGDGGNGGKAGLVGQGGDGGAGGNNNSGFGTGGSGGK 302 GG G G G+ GG G G G G + GG + SG GG G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 303 GGDAVLIGNGGNGGNAGSGGVGPGIAGAA----------GIGGLLIGEDGMA 344 G GNG +GG +G+GG +A G GGL + A Sbjct: 63 GNGG---GNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGA 111 Score = 32.0 bits (72), Expect = 0.004 Identities = 25/81 (30%), Positives = 34/81 (41%), Gaps = 1/81 (1%) Query: 256 AGGDSVGGADGGDGGNGGKAGLVGQGGDGGAGGNNNSGFGTGGSGGKGGDAVLIGNGGNG 315 +GGD G G +G G G GG G ++ SG+ + + GG I GG Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGG-GASDGSGWSSENNPWGGGSGSGIHWGGGS 60 Query: 316 GNAGSGGVGPGIAGAAGIGGL 336 G+ GG G G+ G L Sbjct: 61 GHGNGGGNGNSGGGSGTGGNL 81 Score = 32.0 bits (72), Expect = 0.004 Identities = 28/81 (34%), Positives = 35/81 (43%), Gaps = 4/81 (4%) Query: 132 GAGGSGTPGTASVAGGNGGNGGAGGLLFGTGGAGGAG-GTASSLVGAIPGGNGGYGGDGG 190 G G G A GN NGG GL G G + G+G + ++ G G +GG G Sbjct: 3 GGDGRGHNTGAHSTSGNI-NGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 191 LLFGRGGAGGAGGFGNNTTGG 211 G GG G G G+ T G Sbjct: 62 --HGNGGGNGNSGGGSGTGGN 80 Score = 31.6 bits (71), Expect = 0.004 Identities = 34/108 (31%), Positives = 38/108 (35%), Gaps = 13/108 (12%) Query: 109 DTGRPLIGNGANGGAAGWLIGNGGAGGSGTPGTASVAGGNGGNGGAGGLLFGTGGAGGAG 168 +TG NGG G G G S G +S GG G+G G G G G Sbjct: 10 NTGAHSTSGNINGGPTG---LGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGG 66 Query: 169 GTASSLVGAIPGGNGGYGGDGGLLFGRGGAGGAGGFGNNTTGGIGGLG 216 G GN G G G A A GF +T G GGL Sbjct: 67 G----------NGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 30.5 bits (68), Expect = 0.010 Identities = 32/109 (29%), Positives = 46/109 (42%), Gaps = 9/109 (8%) Query: 238 GNGGDGGTGLFAAGGDSGAGGDSVGGADGGDGGNGGKAGLVGQGGDGGAGGNNNSGFGTG 297 G+G TG + G+ G +G G G+G + GG G+G + G G Sbjct: 4 GDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG-H 62 Query: 298 GSGGKGGDAVLIGNGGNGGNAGSGGVGPGIAGAAGIGGLLIGEDGMAGL 346 G+GG GNG +GG +G+GG +A G + G GL Sbjct: 63 GNGG--------GNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGL 103 Score = 29.7 bits (66), Expect = 0.020 Identities = 29/85 (34%), Positives = 29/85 (34%), Gaps = 7/85 (8%) Query: 162 GGAGGAGGTASSLVGAIPGGNGGYGGDGGLLFGRGGAGGAGGFGNNTTGGIGGLGGAGGL 221 G G A S G I GG G G GG G NN GG G G Sbjct: 4 GDGRGHNTGAHSTSGNINGGPTGLGVGGG-----ASDGSGWSSENNPWGGGSGSGI--HW 56 Query: 222 FGAGGDGGVGGFGTFAGNGGDGGTG 246 G G G GG G G G GG Sbjct: 57 GGGSGHGNGGGNGNSGGGSGTGGNL 81
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_0571	IGASERPTASE	30	0.016	IgA-specific serine endopeptidase (S6) signature.
>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature. Length = 1541 Score = 29.6 bits (66), Expect = 0.016 Identities = 17/51 (33%), Positives = 20/51 (39%), Gaps = 3/51 (5%) Query: 232 VTFDEDRHRAHTGNGAQVLATLRNTAINLHRLNGADNIAEACRITALTANR 282 V E+ H TGN L N I+ LN ADN + LT N Sbjct: 846 VRLTENSHWHLTGNSDVHQLDLANGHIH---LNSADNSNNVTKYNTLTVNS 893
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_0572	cloacin	41	1e-05	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 41.2 bits (96), Expect = 1e-05 Identities = 38/113 (33%), Positives = 46/113 (40%), Gaps = 1/113 (0%) Query: 684 GGAGGDGGSGGTGRGGTGGGGTGGGGTGGGGGVGINNGSGEAIGGAPGAGGTGAVGGDGG 743 G G + G GG G G GGG + G G NN G G GG G GG Sbjct: 8 GHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGG 67 Query: 744 QGGAAYSYGTGDATGSAGAAGTAGTTGVGGTGGAGGAAYTLNGASTATATGGI 796 G + GTG SA AA A T GAGG A +++ + + A I Sbjct: 68 NGNSGGGSGTG-GNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAAIADI 119 Score = 41.2 bits (96), Expect = 2e-05 Identities = 35/104 (33%), Positives = 45/104 (43%), Gaps = 5/104 (4%) Query: 434 GNGGTGGDSGAMGSSGG-RGGDGGVGTNGGAGGGGGNATSYGTANATGGAGGDGGTGSTG 492 G G G ++GA +SG GG G+G GGA G G + + N G G G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSG----WSSENNPWGGGSGSGIHWGG 58 Query: 493 NGGSGGDGGNGGTGHGGGGGPGGTAINYGAGDAFGGAAGKGGTG 536 G G GGNG +G G G G +A+ F + G G Sbjct: 59 GSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG 102 Score = 40.1 bits (93), Expect = 4e-05 Identities = 30/86 (34%), Positives = 37/86 (43%), Gaps = 3/86 (3%) Query: 360 GAGAVAGASGAAGTIIAGNGGNGGAGGAGYAADGPAGPAIGNGGDGGHGGAGGFYGNGGA 419 G G GA +G I NGG G G G A+DG + N GG G + G G Sbjct: 6 GRGHNTGAHSTSGNI---NGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 420 GGAGGNSAPGGGTGGNGGTGGDSGAM 445 G GGN GGG+G G + + Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPV 88 Score = 34.3 bits (78), Expect = 0.002 Identities = 36/124 (29%), Positives = 44/124 (35%), Gaps = 1/124 (0%) Query: 150 GSGAAGQAGGAGGAAGLIGTGGAGGMGGAGGGAGGMGGSGGWLLGNGGAGGAGGVGCAGV 209 G G GA +G I GG G+G GG + G G S GG+G G Sbjct: 3 GGDGRGHNTGAHSTSGNI-NGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 210 SGGVGGTGGNAMLFGNGGAGGMGGAGADGAVGAAGTAGTSTSAGGVGGAGWDATAAGVLA 269 G GG G + G GG A A T G A + A A ++A Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAAIADIMA 121 Query: 270 ATGG 273 A G Sbjct: 122 ALKG 125 Score = 33.9 bits (77), Expect = 0.003 Identities = 25/78 (32%), Positives = 30/78 (38%) Query: 429 GGGTGGNGGTGGDSGAMGSSGGRGGDGGVGTNGGAGGGGGNATSYGTANATGGAGGDGGT 488 G G G N G SG + G GG ++G N G+ + GG G Sbjct: 4 GDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHG 63 Query: 489 GSTGNGGSGGDGGNGGTG 506 GNG SGG G GG Sbjct: 64 NGGGNGNSGGGSGTGGNL 81 Score = 33.5 bits (76), Expect = 0.004 Identities = 31/105 (29%), Positives = 38/105 (36%), Gaps = 2/105 (1%) Query: 632 SGGGADINNGAFTVVPQGGAGGHGGDGATDGGAGGAGGFTEIDSSASVIAATGGAGGDGG 691 SGG +N GG G G G + G+G +E + + GG G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 692 SGGTGRGGTGGGGTGGGGTGGGGGVGINNGSGEAIGGAPGAGGTG 736 G G G GGG+G G G V G PGAGG Sbjct: 62 HGNGGGNGNSGGGSGTG--GNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 32.8 bits (74), Expect = 0.007 Identities = 32/115 (27%), Positives = 38/115 (33%), Gaps = 7/115 (6%) Query: 224 GNGGAGGMGGAGADGAVGAAGTAGTSTSAGGVGGAGWDATAAGVLAATGGDGGDSGGGGA 283 G G G GA + G G G G+GW + +G GG G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 284 GGNGGAGGAGGHGSALFGADGANGNGGAGGAGGNPGAPGNGGTGGVGPDAATSGG 338 G GG G +GG G GN A A G P G G + S G Sbjct: 63 GNGGGNGNSGGG-------SGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAG 110 Score = 32.4 bits (73), Expect = 0.009 Identities = 32/108 (29%), Positives = 37/108 (34%), Gaps = 5/108 (4%) Query: 278 SGGGGAGGNGGAGGAGGHGSALFGADGANGNGGAGGAGGNPGAPGNGGTGGVGPDAATSG 337 SGG G G N GA G+ + G G G GGA G G G + Sbjct: 2 SGGDGRGHNTGAHSTSGNING-----GPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHW 56 Query: 338 GMGGTGGDPGAVGGRGNSGAAGGAGAVAGASGAAGTIIAGNGGNGGAG 385 G G G+ G G G GG + A A G G GG Sbjct: 57 GGGSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 32.0 bits (72), Expect = 0.011 Identities = 27/89 (30%), Positives = 37/89 (41%), Gaps = 6/89 (6%) Query: 384 AGGAGYAADGPAGPAIGNGGDGGHGGAGGFYGNGGAGGAGGNSAPGGGTGGNGGTGGDSG 443 +GG G + A GN G G G + G+G + N+ GGG+G GG Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGG--- 58 Query: 444 AMGSSGGRGGDGGVGTNGGAGGGGGNATS 472 SG G G + GG+G GG + Sbjct: 59 ---GSGHGNGGGNGNSGGGSGTGGNLSAV 84 Score = 30.1 bits (67), Expect = 0.042 Identities = 22/79 (27%), Positives = 30/79 (37%) Query: 752 GTGDATGSAGAAGTAGTTGVGGTGGAGGAAYTLNGASTATATGGIGGNGGDGGTANGSNG 811 G G TG+ +G G G G + + + GG G GG + NG Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNG 65 Query: 812 GNGGAGGYASTTGTGTASV 830 G G G S TG ++V Sbjct: 66 GGNGNSGGGSGTGGNLSAV 84 Score = 30.1 bits (67), Expect = 0.049 Identities = 30/89 (33%), Positives = 37/89 (41%), Gaps = 5/89 (5%) Query: 481 GAGGDGGTGSTGNGGSGGDGGNGGTGHGGGGGPGGTAINYGAGDAFGGAAGKGGTGVVGG 540 G G + G ST +GG G G G G G G ++ N G G GG G Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGG-GASDGSGWSSENNPWGGGSGSGIHWGGGSG-HG 63 Query: 541 NGGSGGAAYNYGTGNATGAGGSSGSGGAA 569 NGG G N G G+ TG S+ + A Sbjct: 64 NGGGNG---NSGGGSGTGGNLSAVAAPVA 89
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_0574	IGASERPTASE	57	3e-10	IgA-specific serine endopeptidase (S6) signature.
>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature. Length = 1541 Score = 57.0 bits (137), Expect = 3e-10 Identities = 47/248 (18%), Positives = 68/248 (27%), Gaps = 12/248 (4%) Query: 726 LDREKATLPEKGTAAKEAEKRAKTAPKAAAPAAPAPAPAEA-PAKAAEASAAATAASPAA 784 +D T P A + + A AP P PA A P++ E A + Sbjct: 992 VDTTNITTPNNIQADVPSV-PSNNEEIARVDEAPVPPPAPATPSETTETVAENSKQESKT 1050 Query: 785 PAKGLGMAGGAK---RPGAKKAAPAPAAETAAAEAPAAPAKGLGMAAGAKKPGAKKAAAP 841 K A R AK+A A T E A+ + K+ A Sbjct: 1051 VEKNEQDATETTAQNREVAKEAKSNVKANTQTNEV----AQSGSETKETQTTETKETATV 1106 Query: 842 TGETKPAEAAAPAAPVKGLGMASGAKRPGAKKAAPPAAAAPEAAATAPAPEAAA---APA 898 E K V + K+ ++ P A A E T E + A Sbjct: 1107 EKEEKAKVETEKTQEVPKVTSQVSPKQEQSETVQPQAEPARENDPTVNIKEPQSQTNTTA 1166 Query: 899 EPAAPAAPVKGLGIATGAKRPGAKKAPARAEAPSAAAPAQPEPEATPEPEPASKQDGEPT 958 + PA + + E P PA +P E K + Sbjct: 1167 DTEQPAKETSSNVEQPVTESTTVNTGNSVVENPENTTPATTQPTVNSESSNKPKNRHRRS 1226 Query: 959 PPAAPAAP 966 + P Sbjct: 1227 VRSVPHNV 1234 Score = 42.4 bits (99), Expect = 9e-06 Identities = 35/199 (17%), Positives = 60/199 (30%), Gaps = 15/199 (7%) Query: 698 TDGVNDRQEEAGRSGVEV----LDVAQVLLGSLDREKATLPEKGTAAKEAEKRAK--TAP 751 T N + +S V+ +VAQ GS +E T K TA E E++AK T Sbjct: 1061 TTAQNREVAKEAKSNVKANTQTNEVAQS--GSETKETQTTETKETATVEKEEKAKVETEK 1118 Query: 752 KAAAPAAPAPAPAEAPAKAAEASAAATAASPAAPAKGLGMAGGAKRPGAKKAAPAPAAET 811 P ++ K ++ A PA + A A+ Sbjct: 1119 TQEVPK----VTSQVSPKQEQSETVQPQAEPARENDPTVNIKEPQSQTNTTADTEQPAKE 1174 Query: 812 AAAEAPAAPAKGLGMAAGAKKPGAKKAAAPTGETKPAEAAAPAAPVKGLGMASGAKRPGA 871 ++ + + G + P T+P + + K S R Sbjct: 1175 TSSNVEQPVTESTTVNTGNSVVENPENTTPA-TTQPTVNSESSNKPKNRHRRS--VRSVP 1231 Query: 872 KKAAPPAAAAPEAAATAPA 890 P ++ + + A Sbjct: 1232 HNVEPATTSSNDRSTVALC 1250
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_0579	PF03544	39	5e-05	Gram-negative bacterial tonB protein
>PF03544#Gram-negative bacterial tonB protein Length = 243 Score = 39.2 bits (91), Expect = 5e-05 Identities = 21/75 (28%), Positives = 22/75 (29%), Gaps = 10/75 (13%) Query: 878 NPPVINAPAPHVATPTPAHTPPVEHAPVVTPQPHVPAEQPAHHEPPSPSVFGHEPPVTHT 937 P + P P P P VE P P P P E P E P P P Sbjct: 56 APADL--EPPQAVQPPPE--PVVEPEPEPEPIPEPPKEAPVVIEKPKP------KPKPKP 105 Query: 938 PPVHVDPPSHGPVDP 952 PV V P Sbjct: 106 KPVKKVEQPKRDVKP 120
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_0582	SHAPEPROTEIN	38	9e-05	Bacterial cell shape determinant MreB/Mbl protein s...
>SHAPEPROTEIN#Bacterial cell shape determinant MreB/Mbl protein signature. Length = 347 Score = 37.8 bits (88), Expect = 9e-05 Identities = 55/245 (22%), Positives = 86/245 (35%), Gaps = 56/245 (22%) Query: 112 SGAQLGALRGALLTEPALAPNGVAAAVVSDAAAALAALRPTPGFPASGVVALCDFGAGGT 171 S GA L+ EP +AAA+ A L P A+G + + D G G T Sbjct: 129 SAQGAGAREVFLIEEP------MAAAI----GAGL------PVSEATGSM-VVDIGGGTT 171 Query: 172 SVTLAQVG----SSLQQIGPTFRYREFSGDEIDQLILNHI---LTVTPGIDSAEVSGTAT 224 V + + SS +IG GD D+ I+N++ G +AE Sbjct: 172 EVAVISLNGVVYSSSVRIG---------GDRFDEAIINYVRRNYGSLIGEATAE------ 216 Query: 225 SMGSVTLLLGGCRFAKEHLSA-APVATIATGAAGQPGADIRFSRNEFEQLITQPLDRFIG 283 + +G E +A G + NE + + +PL + Sbjct: 217 ---RIKHEIGSAYPGDEVREIEVRGRNLAEGVP----RGFTLNSNEILEALQEPLTGIVS 269 Query: 284 SVEDMLQRSGVPRPSLAA------VAAVGGGAAIPLIGNRLSERLQVPVFTTAQPIFSAA 337 +V L++ P LA+ + GGGA + + L E +PV P+ A Sbjct: 270 AVMVALEQC---PPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVA 326 Query: 338 IGAAM 342 G Sbjct: 327 RGGGK 331
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_0583	PYOCINKILLER	28	0.025	Pyocin S killer protein signature.
>PYOCINKILLER#Pyocin S killer protein signature. Length = 617 Score = 27.8 bits (61), Expect = 0.025 Identities = 18/99 (18%), Positives = 29/99 (29%) Query: 73 PTPASVTKTVTATMTTTTPTTTTAPTKTTTTTTTTTTTTTTTTTTTTTTTTTTTPTTTTT 132 + V+ + TT + T +TT T T + P++TT Sbjct: 373 VSTDGVSVPKAVPVRMAAYNATTGLYEVTVPSTTAEAPPLILTWTPASPPGNQNPSSTTP 432 Query: 133 TTTAPTTTTTTTTNPMSPGAMPTFPSQLTPSIPTVINLP 171 P T T+P +T +I P Sbjct: 433 VVPKPVPVYEGATLTPVKATPETYPGVITLPEDLIIGFP 471
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_0585	PF05616	31	0.003	Neisseria meningitidis TspB protein
>PF05616#Neisseria meningitidis TspB protein Length = 501 Score = 30.9 bits (69), Expect = 0.003 Identities = 23/87 (26%), Positives = 33/87 (37%), Gaps = 8/87 (9%) Query: 32 PIATPGAGPTEPSFPTRRPTTSPPTSTSP-SQPTSPASPTSPAGAIPLPPDDNGYVFIET 90 P PG P P P +P T P ++P SPA P P G + E Sbjct: 343 PNENPGTRPNPEPDPDLNPDANPDTDGQPGTRPDSPAVPDRPNGR-------HRKERKEG 395 Query: 91 KSGQTRCQINHDSVGCEAPFTNSPIKD 117 + G C+ D + C+ +P +D Sbjct: 396 EDGGLLCKFFPDILACDRLPEPNPAED 422
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_0593	SHAPEPROTEIN	136	2e-37	Bacterial cell shape determinant MreB/Mbl protein s...
>SHAPEPROTEIN#Bacterial cell shape determinant MreB/Mbl protein signature. Length = 347 Score = 136 bits (344), Expect = 2e-37 Identities = 73/368 (19%), Positives = 141/368 (38%), Gaps = 66/368 (17%) Query: 2 ARAVGIDLGTTNSVVAVLEGGDP-----VVVANSEGSRTTPSVVAFARNGEVLVGQPAKN 56 + + IDLGT N+++ V G VV + + + SV A VG AK Sbjct: 10 SNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQDRAGSPKSVAA--------VGHDAKQ 61 Query: 57 QAVTNVE--RTMRSVKRHMGGDWSIEIDDKKYTTPEISARVLMKLKRDAEAYLGEDIADA 114 +R +K + D+ + T ++ + ++ ++ Sbjct: 62 MLGRTPGNIAAIRPMKDGVIADF--------FVTEKMLQHFIKQVHSNS---FMRPSPRV 110 Query: 115 VITVPAYFNDAQRQATKDAGQIAGLNVLRIVNEPTAAALAYGLDKGEKDQTILVFDLGGG 174 ++ VP +R+A +++ Q AG + ++ EP AAA+ GL + +V D+GGG Sbjct: 111 LVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPV-SEATGSMVVDIGGG 169 Query: 175 TFDVSLLEIGEGVVEVRATSGDNHLGGDDWDDRVVEWLVDKFKGTSGIDLTKDKMAMQRL 234 T +V+++ + V S +GGD +D+ ++ ++ + G Sbjct: 170 TTEVAVISLNGVV-----YSSSVRIGGDRFDEAIINYVRRNYGSLIG------------- 211 Query: 235 REAAEKAKIELSSS----QSTSINLPYITVDAD--KNPLFLDEQLTRAEFQRITQDL--- 285 AE+ K E+ S+ + I + + + ++ A + +T + Sbjct: 212 EATAERIKHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAV 271 Query: 286 ---LDRTRKPFQSVIADTGISVSDIDHVVLVGGSTRMPAVTELVKELTGGKEPNKGVNPD 342 L++ S I++ G+ VL GG + + L+ E T G +P Sbjct: 272 MVALEQCPPELASDISERGM--------VLTGGGALLRNLDRLLMEET-GIPVVVAEDPL 322 Query: 343 EVVAVGAA 350 VA G Sbjct: 323 TCVARGGG 330

MUL_0668

MUL_0681

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
MUL_0668	0	12	1.195713	transcriptional regulator
MUL_0670	0	12	1.488018	hypothetical protein
MUL_0671	0	12	1.663261	hypothetical protein
MUL_0673	-1	11	1.138502	ubiquinone/menaquinone biosynthesis
MUL_0674	0	12	-0.672685	hypothetical protein
MUL_0675	0	13	-0.823005	methyltransferase
MUL_0676	0	14	-0.760887	FAD-linked oxidoreductase
MUL_0677	-2	11	-1.353739	polyprenyl diphosphate synthetase, GrcC1
MUL_0678	-1	11	-1.752341	heat shock protein HtpX
MUL_0679	1	12	-0.473478	transmembrane carbonic anhydrase, SulP
MUL_0681	1	13	-0.154964	transmembrane carbonic anhydrase, SulP

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_0668	V8PROTEASE	28	0.036	V8 serine protease family signature.
>V8PROTEASE#V8 serine protease family signature. Length = 336 Score = 27.7 bits (61), Expect = 0.036 Identities = 19/107 (17%), Positives = 39/107 (36%), Gaps = 23/107 (21%) Query: 83 KGDVALLRVDRR--------NMPSSELTTDADVSIGTRILAVGYPKSTQDITDPSLDPTY 134 +GD+A+++ + + ++ +A+ + I GYP Sbjct: 159 EGDLAIVKFSPNEQNKHIGEVVKPATMSNNAETQVNQNITVTGYP------------GDK 206 Query: 135 KSGTVSKKSLRQTIPEYE---IDAPISDGMSGGPTIELNGKVIGLNS 178 T+ + + T + E D + G SG P +VIG++ Sbjct: 207 PVATMWESKGKITYLKGEAMQYDLSTTGGNSGSPVFNEKNEVIGIHW 253
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_0675	HTHTETR	66	2e-15	TetR bacterial regulatory protein HTH signature.
>HTHTETR#TetR bacterial regulatory protein HTH signature. Length = 215 Score = 65.8 bits (160), Expect = 2e-15 Identities = 30/184 (16%), Positives = 70/184 (38%), Gaps = 10/184 (5%) Query: 5 AERGAQTRAALMAAAVAVIAERGWGAATTRMVAERAGLPPGLVHYHFASLNDLL---IDA 61 + +TR ++ A+ + +++G + + +A+ AG+ G +++HF +DL + Sbjct: 6 KQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSEIWEL 65 Query: 62 ALQAAREEAAQVLDGLAGDSSSQGIDRLIDAVSSYDVDDRNQNPAILVFGEMLLAATRYE 121 + E + GD S + LI + S ++R + ++F + Sbjct: 66 SESNIGELELEYQAKFPGDPLSVLREILIHVLESTVTEERRRLLMEIIFHKCEFVGEMAV 125 Query: 122 RLRMGLAEILGDYRSTLRQWLAD--QGGAI----DPEATAALMFAAIDGLVLHRVIDPRL 175 + + + + Q L + + A +M I GL+ + + P+ Sbjct: 126 VQQAQ-RNLCLESYDRIEQTLKHCIEAKMLPADLMTRRAAIIMRGYISGLMENWLFAPQS 184 Query: 176 RTLA 179 L Sbjct: 185 FDLK 188
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_0676	DHBDHDRGNASE	112	9e-32	2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase signature. Length = 261 Score = 112 bits (280), Expect = 9e-32 Identities = 69/251 (27%), Positives = 118/251 (47%), Gaps = 17/251 (6%) Query: 3 ALDGRVALITGGARGQGRAHALALAGKGADIALADAPGPMAELTYPLGSEEDLLATAELV 62 ++G++A ITG A+G G A A LA +GA IA D + E L + Sbjct: 5 GIEGKIAFITGAAQGIGEAVARTLASQGAHIAAVDY------------NPEKLEKVVSSL 52 Query: 63 GQLGRRCLPMVVDVRDPAQVNTAVERTVRELGSLDIVLANAGIVSTGRLEEVSDQVWQQL 122 R DVRD A ++ R RE+G +DI++ AG++ G + +SD+ W+ Sbjct: 53 KAEARHAEAFPADVRDSAAIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEAT 112 Query: 123 MDTNLTGVFHTLRAAIPVMRQQRFGRIVATSSMGGRMGIPELAAYNATKWGIIGLIKSVA 182 N TGVF+ R+ M +R G IV S + +AAY ++K + K + Sbjct: 113 FSVNSTGVFNASRSVSKYMMDRRSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLG 172 Query: 183 LEVAKEGITANVICPTTTQTPMMQPAGIGDDQEVPDDLVRRMMKANPIPQPW---LQPED 239 LE+A+ I N++ P +T+T M ++ + +++ ++ P +P D Sbjct: 173 LELAEYNIRCNIVSPGSTETDMQWSLWADENGA--EQVIKGSLETFKTGIPLKKLAKPSD 230 Query: 240 VSRGVVYLVTD 250 ++ V++LV+ Sbjct: 231 IADAVLFLVSG 241
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_0677	ACRIFLAVINRP	29	0.037	Acriflavin resistance protein family signature.
>ACRIFLAVINRP#Acriflavin resistance protein family signature. Length = 1034 Score = 29.0 bits (65), Expect = 0.037 Identities = 17/71 (23%), Positives = 32/71 (45%), Gaps = 11/71 (15%) Query: 54 PIVVALADVALFTVLFTDGQRANVRELRETWTLSGRALGVGMPLTMIGIAVPAHFLTGLN 113 P +VA++ V +F L L E+W++ + + +PL ++G + A L Sbjct: 873 PALVAISFVVVFLCLAA---------LYESWSIPVSVM-LVVPLGIVG-VLLAATLFNQK 921 Query: 114 WPTAFLVGAIL 124 F+VG + Sbjct: 922 NDVYFMVGLLT 932
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_0678	IGASERPTASE	30	0.019	IgA-specific serine endopeptidase (S6) signature.
>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature. Length = 1541 Score = 30.0 bits (67), Expect = 0.019 Identities = 17/51 (33%), Positives = 20/51 (39%), Gaps = 3/51 (5%) Query: 283 VTFDEDRHRAHTGNGAQVLATLRNTAINLHRLNGADNIAEACRITALTANR 333 V E+ H TGN L N I+ LN ADN + LT N Sbjct: 846 VRLTENSHWHLTGNSDVHQLDLANGHIH---LNSADNSNNVTKYNTLTVNS 893
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_0681	HTHTETR	53	7e-11	TetR bacterial regulatory protein HTH signature.
>HTHTETR#TetR bacterial regulatory protein HTH signature. Length = 215 Score = 53.1 bits (127), Expect = 7e-11 Identities = 28/183 (15%), Positives = 58/183 (31%), Gaps = 22/183 (12%) Query: 19 AVRRDDRILDIVVHLLQTEGYDAVQLREVARRARTSLATIYKRYANRDELILAALEFWMD 78 A ILD+ + L +G + L E+A+ A + IY + ++ +L E + Sbjct: 9 AQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSEIWE--LS 66 Query: 79 EHHYAGLAEQTPAPGECLYAGMMRVLRMIFQPWETHPDIVKAYFRARAAPGGQRLVHRGL 138 E + L + A + P +I+ + +RL Sbjct: 67 ESNIGELELEYQA-------------KFPGDPLSVLREILIHVLESTVTEERRRL----- 108 Query: 139 DMVVPAAMEVLAGVEEDFIHDLDTVISSLVYGLVGRFTAGEIAITEILPSID-RTVFWLI 197 ++ + E + + Y + + I + + R ++ Sbjct: 109 -LMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMTRRAAIIM 167 Query: 198 RGY 200 RGY Sbjct: 168 RGY 170

MUL_0740

MUL_0747

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
MUL_0740	0	15	2.472703	transcription antitermination protein NusG
MUL_0741	3	20	-0.370323	50S ribosomal protein L11
MUL_0742	5	24	-0.584058	50S ribosomal protein L1
MUL_0743	5	22	-0.593610	methoxy mycolic acid synthase 4, MmaA4
MUL_0744	4	23	-0.700542	methoxy mycolic acid synthase 3, MmaA3
MUL_0745	4	24	-0.787734	methoxy mycolic acid synthase 2, MmaA2
MUL_0746	3	22	-0.326534	lipase/esterase
MUL_0747	1	18	0.083458	hypothetical protein

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_0740	cloacin	41	2e-05	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 40.9 bits (95), Expect = 2e-05 Identities = 31/100 (31%), Positives = 34/100 (34%) Query: 446 GAGGRGGDGGAYGNGGVSGNGGAGGAGSPGAHGGTAGEDGFNAGDGGHGGAGGAGGGGGA 505 G GRG + GA+ G G G GA G+ N GG G GGG G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 506 KGGVGGAGGSGGVGGQGGTGGDGATGLSGKAGTGTDGESG 545 G G GG G G A G T G G Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG 102 Score = 36.2 bits (83), Expect = 5e-04 Identities = 40/116 (34%), Positives = 47/116 (40%), Gaps = 11/116 (9%) Query: 133 GSGQAGGNGGLLWGNGGNGGSGGVGQAGGAGGSAGLLGHGGAGGAGGVSGVSAVGATGGA 192 G G+ G NGG G+G GGA +G G G SG+ G +G Sbjct: 4 GDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH- 62 Query: 193 GGNGGWLYGNGGAGGLGGQGVLIGGNGGAGGAARFFG--AGGTGGAGGLGLGDTGG 246 GNGG G G G GGN A A FG A T GAGGL + + G Sbjct: 63 --------GNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAG 110 Score = 35.1 bits (80), Expect = 0.001 Identities = 30/91 (32%), Positives = 34/91 (37%), Gaps = 4/91 (4%) Query: 481 AGEDGFNAGDGGHGGAGGAGGGGGAKGGVGGAGGSGGVGGQGGTGGDGATGLSGKAGTGT 540 +G DG G H +G GG G GGA G + G G SG Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGG----SGSGIHWG 57 Query: 541 DGESGGRGGHAGNGGNGGNGGRGGSAHASLV 571 G G GG GN G G G SA A+ V Sbjct: 58 GGSGHGNGGGNGNSGGGSGTGGNLSAVAAPV 88 Score = 34.7 bits (79), Expect = 0.001 Identities = 40/128 (31%), Positives = 50/128 (39%), Gaps = 3/128 (2%) Query: 229 GAGGTGGAGGLGLGDTGGIGGIGGNAGALFGPGGAGGP---GGAGGNGGAGGSGGALADD 285 G G GA GG G+G GA G G + GG G+G G G + Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNG 65 Query: 286 GGDGGAGGAGGIGGNGGAGTTGLAGHIGIDMNGGAGGVGGAGGNGGAGGTGGDAGHAQAG 345 GG+G +GG G GGN A +A GAGG+ + G D A G Sbjct: 66 GGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAAIADIMAALKG 125 Query: 346 GYSDGLQG 353 + GL G Sbjct: 126 PFKFGLWG 133 Score = 34.7 bits (79), Expect = 0.001 Identities = 27/89 (30%), Positives = 32/89 (35%) Query: 492 GHGGAGGAGGGGGAKGGVGGAGGSGGVGGQGGTGGDGATGLSGKAGTGTDGESGGRGGHA 551 G G G G G + G GVGG G ++ + G G G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 552 GNGGNGGNGGRGGSAHASLVGKAGNGGFG 580 GNGG GN G G +L A FG Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFG 91 Score = 33.9 bits (77), Expect = 0.002 Identities = 27/87 (31%), Positives = 36/87 (41%), Gaps = 2/87 (2%) Query: 297 IGGNGGAGTTGLAGHIGIDMNGGAGGVGGAGGNGGAGGTGGDAGHAQAGGYSDGLQGAGG 356 + G G G A ++NGG G+G GG + G+G + + GG S GG Sbjct: 1 MSGGDGRGHNTGAHSTSGNINGGPTGLGVGGG--ASDGSGWSSENNPWGGGSGSGIHWGG 58 Query: 357 DGGHGGSGGVAGDGGRGADAAAGSGLA 383 GHG GG GG S +A Sbjct: 59 GSGHGNGGGNGNSGGGSGTGGNLSAVA 85 Score = 32.8 bits (74), Expect = 0.004 Identities = 33/105 (31%), Positives = 41/105 (39%), Gaps = 1/105 (0%) Query: 216 GGNGGAGGAARFFGAGGTGGAGGLGLGDTGGIGGIGGNAGALFGPGGAGGPGGAGGNGGA 275 G N GA + G TG G G D G G G G G G GNGG Sbjct: 8 GHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGG 67 Query: 276 GGSGGALADDGGDGGAGGAGGIGGNGGAGTTGLAGHIGIDMNGGA 320 G+ G + GG+ A A G T G AG + + ++ GA Sbjct: 68 NGNSGGGSGTGGNLSAVAAPVAFGFPALSTPG-AGGLAVSISAGA 111 Score = 32.4 bits (73), Expect = 0.006 Identities = 36/109 (33%), Positives = 44/109 (40%), Gaps = 4/109 (3%) Query: 366 VAGDGGRGADAAAGSGLAGGDGGRGGDPGAGGEGGAAGGGSVAGTAGLDGIGPNSG-GNG 424 ++G GRG + A S +GG G G GGA+ G + G G SG G Sbjct: 1 MSGGDGRGHNTGAHSTSGNINGGPTG---LGVGGGASDGSGWSSENNPWGGGSGSGIHWG 57 Query: 425 GNGGHGGSGAVGVEGGAGSAGGAGGRGGDGGAYGNGGVSGNGGAGGAGS 473 G GHG G G GG GG A+G +S G G A S Sbjct: 58 GGSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVS 106 Score = 32.4 bits (73), Expect = 0.007 Identities = 27/83 (32%), Positives = 33/83 (39%) Query: 417 GPNSGGNGGNGGHGGSGAVGVEGGAGSAGGAGGRGGDGGAYGNGGVSGNGGAGGAGSPGA 476 G G N G G+ G G G + G G GG SG+G G GS Sbjct: 4 GDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHG 63 Query: 477 HGGTAGEDGFNAGDGGHGGAGGA 499 +GG G G +G GG+ A A Sbjct: 64 NGGGNGNSGGGSGTGGNLSAVAA 86 Score = 32.0 bits (72), Expect = 0.009 Identities = 24/78 (30%), Positives = 31/78 (39%) Query: 335 TGGDAGHAQAGGYSDGLQGAGGDGGHGGSGGVAGDGGRGADAAAGSGLAGGDGGRGGDPG 394 +GGD G +S GG G G GG + G ++ G +G GG G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 395 AGGEGGAAGGGSVAGTAG 412 G GG G +GT G Sbjct: 62 HGNGGGNGNSGGGSGTGG 79 Score = 32.0 bits (72), Expect = 0.009 Identities = 27/79 (34%), Positives = 34/79 (43%) Query: 519 GGQGGTGGDGATGLSGKAGTGTDGESGGRGGHAGNGGNGGNGGRGGSAHASLVGKAGNGG 578 GG G GA SG G G G G G+G + N GG + + + G+G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 579 FGGTGGNSFGGVSGNGGNG 597 G G + GG SG GGN Sbjct: 63 GNGGGNGNSGGGSGTGGNL 81 Score = 31.2 bits (70), Expect = 0.014 Identities = 37/119 (31%), Positives = 48/119 (40%), Gaps = 15/119 (12%) Query: 382 LAGGDGGRGGDPGAGGEGGAAGGGSVAGTAGLDGIGPNSGGNGGNGGHGGSGAVGVEGGA 441 ++GGDG RG + GA G GG G+G G + G+G + G G+ Sbjct: 1 MSGGDG-RGHNTGAHSTSGNINGGPT-------GLGVGGGASDGSGWSSENNPWGGGSGS 52 Query: 442 GSAGGAGGRGGDGGAYGNGGVSGNGGAGGAGSPGAHGGTAGEDGFNAGDGGHGGAGGAG 500 G G G G+GG GN G GG+G+ G A F GAGG Sbjct: 53 GIHWGGGSGHGNGGGNGNSG-------GGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 31.2 bits (70), Expect = 0.014 Identities = 27/90 (30%), Positives = 36/90 (40%), Gaps = 1/90 (1%) Query: 532 LSGKAGTGTDGESGGRGGHAGNGGNGGNGGRGGSAHASLVGKAGNGGFGGTGGNSFGGVS 591 +SG G G + + G+ NGG G G GG++ S N GG+G G Sbjct: 1 MSGGDGRGHNTGAHSTSGNI-NGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGG 59 Query: 592 GNGGNGGDGGHALHGQPGGHGGQGGHGGVA 621 GNGG G++ G G VA Sbjct: 60 SGHGNGGGNGNSGGGSGTGGNLSAVAAPVA 89 Score = 31.2 bits (70), Expect = 0.016 Identities = 37/122 (30%), Positives = 44/122 (36%), Gaps = 22/122 (18%) Query: 236 AGGLGLGDTGGIGGIGGNAGALFGPGGAGGPGGAGGNGGAG-GSGGALADDGGDGGAGGA 294 +GG G G G GN GGP G G GGA GSG + ++ GG+G Sbjct: 2 SGGDGRGHNTGAHSTSGNIN--------GGPTGLGVGGGASDGSGWSSENNPWGGGSGSG 53 Query: 295 GGIGGNGGAGTTGLAGHIGIDMNGGAGGVGGAGGNGGAGGTGGDAGHAQAGGYSDGLQGA 354 GG G G G G GG+G G A G + GA Sbjct: 54 IHWGGGSGHG-------------NGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGA 100 Query: 355 GG 356 GG Sbjct: 101 GG 102
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_0743	HTHTETR	53	1e-10	TetR bacterial regulatory protein HTH signature.
>HTHTETR#TetR bacterial regulatory protein HTH signature. Length = 215 Score = 53.1 bits (127), Expect = 1e-10 Identities = 19/106 (17%), Positives = 38/106 (35%), Gaps = 3/106 (2%) Query: 7 SPTQRSGVRDEMLHAAVALLDAHGPDALQTRKVAGATGTSTMAVYTHFGGMPELIAEVAD 66 + + R +L A+ L G + ++A A G + A+Y HF +L +E+ + Sbjct: 5 TKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSEIWE 64 Query: 67 EG---LRQFDTALAVPPSDDPVADLVATGAAYRRYAIERPHMYRLM 109 + + + DP++ L + LM Sbjct: 65 LSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVTEERRRLLM 110
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_0745	PF05272	28	0.049	Virulence-associated E family protein
>PF05272#Virulence-associated E family protein Length = 892 Score = 28.5 bits (63), Expect = 0.049 Identities = 10/22 (45%), Positives = 13/22 (59%) Query: 32 VSVLLGPSGTGKSVFLKSLIGL 53 VL G G GKS + +L+GL Sbjct: 598 SVVLEGTGGIGKSTLINTLVGL 619
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_0747	GPOSANCHOR	33	0.007	Gram-positive coccus surface protein anchor signature.
>GPOSANCHOR#Gram-positive coccus surface protein anchor signature. Length = 539 Score = 33.5 bits (76), Expect = 0.007 Identities = 23/153 (15%), Positives = 47/153 (30%), Gaps = 2/153 (1%) Query: 147 ELSTLEAEMMVERKAVEDQRDA--DLEARAQKLEADLAELEAEGAKADARRKVRDSGERE 204 E + L A KA+E + A+ + LEA+ A LEA A+ + + + Sbjct: 149 EKAALAARKADLEKALEGAMNFSTADSAKIKTLEAEKAALEARQAELEKALEGAMNFSTA 208 Query: 205 MRQLRDRAQRELDRLEDIWSTFTKLAPKQLIVDENLYRELQDRYGEYFTGAMGAESIQKL 264 + E L + K + +++ E ++K Sbjct: 209 DSAKIKTLEAEKAALAARKADLEKALEGAMNFSTADSAKIKTLEAEKAALEARQAELEKA 268 Query: 265 IETFDIDAEAEILRDVIRNGKGQKKLRALKRLK 297 +E + A+ + + L+ Sbjct: 269 LEGAMNFSTADSAKIKTLEAEKAALEAEKADLE 301

MUL_0762

MUL_0771

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
MUL_0762	5	24	-1.945440	PE-PGRS family protein
MUL_0763	5	24	-2.246392	endonuclease IV
MUL_0764	4	18	-1.572433	lipoprotein LpqP
MUL_0765	3	15	-1.289546	acyl-CoA dehydrogenase
MUL_0766	1	10	-0.630508	enoyl-CoA hydratase
MUL_0767	-1	8	-0.297330	putative regulatory protein
MUL_0768	-1	8	0.265414	enoyl-CoA hydratase
MUL_0769	-2	11	0.244836	transmembrane transport protein MmpL
MUL_0770	-2	13	0.092726	transmembrane proteinm, MmpS5
MUL_0771	-2	15	0.184584	transcriptional regulator

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_0762	TETREPRESSOR	48	3e-09	Tetracycline repressor protein signature.
>TETREPRESSOR#Tetracycline repressor protein signature. Length = 218 Score = 48.4 bits (115), Expect = 3e-09 Identities = 20/44 (45%), Positives = 30/44 (68%) Query: 24 AKLSRDAIVDGALTFLDREGWDSLTINALATQLGTKGPSLYNHV 67 A+L+R++++D AL L+ G D LT LA +LG + P+LY HV Sbjct: 2 ARLNRESVIDAALELLNETGIDGLTTRKLAQKLGIEQPTLYWHV 45
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_0765	TCRTETOQM	588	0.0	Tetracycline resistance protein TetO/TetQ/TetM family ...
>TCRTETOQM#Tetracycline resistance protein TetO/TetQ/TetM family signature. Length = 639 Score = 588 bits (1517), Expect = 0.0 Identities = 163/676 (24%), Positives = 303/676 (44%), Gaps = 71/676 (10%) Query: 12 KVRNIGIMAHIDAGKTTTTERILYYTGISYKIGEVHDGAATMDWMEQEQERGITITSAAT 71 K+ NIG++AH+DAGKTT TE +LY +G ++G V G D E++RGITI + T Sbjct: 2 KIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTDNTLLERQRGITIQTGIT 61 Query: 72 TCFWNDNQINIIDTPGHVDFTVEVERSLRVLDGAVAVFDGKEGVEPQSEQVWRQADKYDV 131 + W + ++NIIDTPGH+DF EV RSL VLDGA+ + K+GV+ Q+ ++ K + Sbjct: 62 SFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAKDGVQAQTRILFHALRKMGI 121 Query: 132 PRICFVNKMDKIGADFYFSVRTMEERLGANVIPIQLPVGSEGDFEGVVDLVEMKAKVWSA 191 P I F+NK+D+ G D + ++E+L A ++ Q Sbjct: 122 PTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQ------------------------- 156 Query: 192 DAKLGEKYDVVDIPADLQEKADEYRTKLLEAVAETDEALLEKYLGGEELTEAEIKGAIRK 251 +L V + Q + V E ++ LLEKY+ G+ L E++ Sbjct: 157 KVELYPNMCVTNFTESEQ----------WDTVIEGNDDLLEKYMSGKSLEALELEQEESI 206 Query: 252 LTITSEAYPVLCGSAFKNKGVQPMLDAVIDYLPSPLDVPAAIGHVPGKEDEEVVRKPSTD 311 +PV GSA N G+ +++ + + S Sbjct: 207 RFHNCSLFPVYHGSAKNNIGIDNLIEVITNKFYSSTH--------------------RGQ 246 Query: 312 EPFSALAFKVATHPFFGKLTYVRVYSGKVDSGSQVINSTKGKKERLGKLFQMHSNKESPV 371 FK+ +L Y+R+YSG + V S K K ++ +++ + + + Sbjct: 247 SELCGKVFKIEYSEKRQRLAYIRLYSGVLHLRDSVRISEKEKI-KITEMYTSINGELCKI 305 Query: 372 ETASAGHIYAVIG----LKDTTTGDTLSDPNNQIVLESMTFPDPVIEVAIEPKTKSDQEK 427 + A +G I + L GDT P E + P P+++ +EP +E Sbjct: 306 DKAYSGEIVILQNEFLKLNS-VLGDTKLLPQR----ERIENPLPLLQTTVEPSKPQQREM 360 Query: 428 LSLSIQKLAEEDPTFKVHLDQETGQTVIGGMGELHLDILVDRMRREFKVEANVGKPQVAY 487 L ++ ++++ DP + ++D T + ++ +G++ +++ ++ ++ VE + +P V Y Sbjct: 361 LLDALLEISDSDPLLRYYVDSATHEIILSFLGKVQMEVTCALLQEKYHVEIEIKEPTVIY 420 Query: 488 KETIKRLVEKVEFTHKKQTGGSGQFAKVLISIEPFTGEDGATYEFESKVTGGRIPREYIP 547 E + K E+T + + +A + +S+ P G+ ++ES V+ G + + + Sbjct: 421 MERPLK---KAEYTIHIEVPPNPFWASIGLSVSP--LPLGSGMQYESSVSLGYLNQSFQN 475 Query: 548 SVDAGAQDAMQYGVLAGYPLVNLKVTLLDGAFHEVDSSEMAFKIAGSQVLKKAAAAAHPV 607 +V G + + G L G+ + + K+ G ++ S+ F++ VL++ A Sbjct: 476 AVMEGIRYGCEQG-LYGWNVTDCKICFKYGLYYSPVSTPADFRMLAPIVLEQVLKKAGTE 534 Query: 608 ILEPIMAVEVTTPEDYMGDVIGDLNSRRGQIQAMEERSGARVVKAHVPLSEMFGYVGDLR 667 +LEP ++ ++ P++Y+ D I + ++ ++ +P + Y DL Sbjct: 535 LLEPYLSFKIYAPQEYLSRAYTDAPKYCANIVDTQLKNNEVILSGEIPARCIQEYRSDLT 594 Query: 668 SKTQGRANYSMVFDSY 683 T GR+ Y Sbjct: 595 FFTNGRSVCLTELKGY 610
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_0766	TCRTETOQM	80	2e-18	Tetracycline resistance protein TetO/TetQ/TetM family ...
>TCRTETOQM#Tetracycline resistance protein TetO/TetQ/TetM family signature. Length = 639 Score = 79.9 bits (197), Expect = 2e-18 Identities = 53/155 (34%), Positives = 82/155 (52%), Gaps = 13/155 (8%) Query: 13 VNIGTIGHVDHGKTTLTAAITKVLH-----DKYPELNESRAFDQIDNAPEERQRGITINI 67 +NIG + HVD GKTTLT ++ L+ + +++ + DN ERQRGITI Sbjct: 4 INIGVLAHVDAGKTTLTESL---LYNSGAITELGSVDKGTT--RTDNTLLERQRGITIQT 58 Query: 68 SHVEYQTEKRHYAHVDAPGHADYIKNMITGAAQMDGAILVVAATDGPMPQTREHVLLARQ 127 +Q E +D PGH D++ + + +DGAIL+++A DG QTR R+ Sbjct: 59 GITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAKDGVQAQTRILFHALRK 118 Query: 128 VGVPYILVALNKSDAVDDEELLELVEMEVRELLAA 162 +G+P I +NK D + L V +++E L+A Sbjct: 119 MGIPTI-FFINKIDQNGID--LSTVYQDIKEKLSA 150
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_0768	DHBDHDRGNASE	92	2e-24	2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase signature. Length = 261 Score = 92.0 bits (228), Expect = 2e-24 Identities = 77/249 (30%), Positives = 113/249 (45%), Gaps = 25/249 (10%) Query: 3 LAREGADIIAMDICGPVSESITYAPATAADLSETIRAVESEGRKVLARQADVRDSAALQQ 62 LA +GA I A+D Y P + +++A E R A ADVRDSAA+ + Sbjct: 28 LASQGAHIAAVD----------YNPEKLEKVVSSLKA---EARHAEAFPADVRDSAAIDE 74 Query: 63 LVADGVEEFGRLDVMVANAGVFGWGRLWELTDEQWDTVIGVNLSGTWRTLRAAVPAMIEA 122 + A E G +D++V AGV G + L+DE+W+ VN +G + R+ M Sbjct: 75 ITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGVFNASRSVSKYM-MD 133 Query: 123 GNGGSIIVVSSSAGLKATPGNGHYAASKHGLVALTNTLAIELGEYDIGVNSIHPYSVETP 182 GSI+ V S+ YA+SK V T L +EL EY+I N + P S ET Sbjct: 134 RRSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLELAEYNIRCNIVSPGSTETD 193 Query: 183 M-----IEPDVMMQVF-GEHPRFLHSFPPMPLQYKGLMTSEEVSDVVVWLAGDGSGTLSG 236 M + + QV G F P K L +++D V++L +G ++ Sbjct: 194 MQWSLWADENGAEQVIKGSLETFKTGIP-----LKKLAKPSDIADAVLFLVSGQAGHITM 248 Query: 237 AQIPVDKGA 245 + VD GA Sbjct: 249 HNLCVDGGA 257
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_0771	HTHTETR	94	5e-26	TetR bacterial regulatory protein HTH signature.
>HTHTETR#TetR bacterial regulatory protein HTH signature. Length = 215 Score = 93.5 bits (232), Expect = 5e-26 Identities = 34/203 (16%), Positives = 76/203 (37%), Gaps = 15/203 (7%) Query: 4 ESRAGRRPSTTKRHIADVAIDLFAARTFAEVSVDDVAQAAGIARRTLFRYYASKNAIPWG 63 + + T++HI DVA+ LF+ + + S+ ++A+AAG+ R ++ ++ K+ + Sbjct: 2 ARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSE 61 Query: 64 DFDTHLAQLQDLLERIDGHVR--LGKALREALLAFNTYDESETIRHRQRMRIILQTAELQ 121 ++ + + +L LRE L+ +E R+ + I+ Sbjct: 62 IWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVTE--ERRRLLMEIIFHKCEF 119 Query: 122 AYSMTMYAGWRAVIAGFV----------ARRLSVKPTDLVPQTVAWTMLGVALSAYEHW- 170 M + + + + P DL+ + A M G E+W Sbjct: 120 VGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMTRRAAIIMRGYISGLMENWL 179 Query: 171 LSDESVSLPEALGNAFDVVGAGL 193 + +S L + + ++ Sbjct: 180 FAPQSFDLKKEARDYVAILLEMY 202

MUL_0883

MUL_0911

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
MUL_0883	-3	12	1.193436	hypothetical protein
MUL_0884	1	13	0.051854	hypothetical protein
MUL_0885	1	13	-0.769467	hypothetical protein
MUL_0886	1	10	-1.269383	glucosamine--fructose-6-phosphate
MUL_0888	2	12	-1.968740	transposase for IS2404
MUL_0889	2	12	-1.986303	hypothetical protein
MUL_0890	1	12	-2.541039	transmembrane protein
MUL_0891	0	15	-1.251999	glutamate decarboxylase
MUL_0894	-1	13	-1.241863	alanine racemase
MUL_0895	-1	11	0.410085	hydrolase
MUL_0896	-2	9	0.929047	hypothetical protein
MUL_0897	3	9	0.792869	hypothetical protein
MUL_0898	3	11	0.851737	ribosomal-protein-alanine acetyltransferase,
MUL_0899	3	12	0.285242	putative DNA-binding/iron metalloprotein/AP
MUL_0900	3	12	0.440175	co-chaperonin GroES
MUL_0901	2	13	0.434670	chaperonin GroEL
MUL_0902	3	10	-0.619018	transposase for IS2404
MUL_0903	0	9	-0.272555	hypothetical protein
MUL_0904	0	9	-0.376720	PPE family protein
MUL_0905	1	10	0.126754	hypothetical protein
MUL_0906	0	9	0.365984	metal-dependent hydrolase
MUL_0907	-1	11	0.407273	Whib-like regulatory protein, WhiB3
MUL_0908	-3	10	1.726148	transposase for IS2404
MUL_0909	-2	10	1.866768	RNA polymerase sigma factor SigD
MUL_0910	-1	10	2.147722	hypothetical protein
MUL_0911	-2	11	2.743206	hypothetical protein

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_0883	SACTRNSFRASE	47	3e-09	Streptothricin acetyltransferase signature.
>SACTRNSFRASE#Streptothricin acetyltransferase signature. Length = 173 Score = 46.9 bits (111), Expect = 3e-09 Identities = 20/93 (21%), Positives = 32/93 (34%), Gaps = 8/93 (8%) Query: 53 GARCADNLVGYAGV-SRLGRVAPFEYEIHTIGVDPAYQGRGIGRRLLDELLAFA---DGG 108 +N +G + S A I I V Y+ +G+G LL + + +A Sbjct: 69 LYYLENNCIGRIKIRSNWNGYA----LIEDIAVAKDYRKKGVGTALLHKAIEWAKENHFC 124 Query: 109 VVFLEVRTDNEPAIALYRSVGFEQVGLRRRYYR 141 + LE + N A Y F + Y Sbjct: 125 GLMLETQDINISACHFYAKHHFIIGAVDTMLYS 157
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_0888	IGASERPTASE	30	0.019	IgA-specific serine endopeptidase (S6) signature.
>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature. Length = 1541 Score = 30.0 bits (67), Expect = 0.019 Identities = 17/51 (33%), Positives = 20/51 (39%), Gaps = 3/51 (5%) Query: 283 VTFDEDRHRAHTGNGAQVLATLRNTAINLHRLNGADNIAEACRITALTANR 333 V E+ H TGN L N I+ LN ADN + LT N Sbjct: 846 VRLTENSHWHLTGNSDVHQLDLANGHIH---LNSADNSNNVTKYNTLTVNS 893
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_0889	cloacin	27	0.042	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 26.6 bits (58), Expect = 0.042 Identities = 21/65 (32%), Positives = 24/65 (36%), Gaps = 6/65 (9%) Query: 79 GPGGWGGPGGPGGPPASGEPYGPYGPQGGAPGTPGWPGGIAGPTAAGGHAGMGGIGGMDG 138 GP G G GG P+G GG+ W GG + G G G GG G Sbjct: 23 GPTGLGVGGGASDGSGWSSENNPWG--GGSGSGIHWGGG----SGHGNGGGNGNSGGGSG 76 Query: 139 MGGMG 143 GG Sbjct: 77 TGGNL 81
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_0890	cloacin	35	8e-04	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 35.1 bits (80), Expect = 8e-04 Identities = 28/100 (28%), Positives = 38/100 (38%), Gaps = 6/100 (6%) Query: 187 NANLGSGNTGIGNIGVGNSGEGNSALVPPQSGNYNIGGGNNGNNNLGAGNIGNFNFGFGN 246 + N+ G TG+G G + G G S+ P G G G + G GN G G Sbjct: 17 SGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGS--GHGNGGGNGNSGGG 74 Query: 247 NGTGNFGFGNAGPADLSNPNLFTFHVTPGENNIGIGNTGN 286 +GTG A P P L TPG + + + Sbjct: 75 SGTGGNLSAVAAPVAFGFPAL----STPGAGGLAVSISAG 110 Score = 29.3 bits (65), Expect = 0.049 Identities = 22/81 (27%), Positives = 32/81 (39%), Gaps = 3/81 (3%) Query: 341 GSGNIGIGNSGSNNIGFFNSGDGNIGAFSSGTNSVFPGQLNSFGVGNSGTGNLGFGNAGS 400 G G+ +S S NI N G +G ++ N+ G SG+G G +G Sbjct: 6 GRGHNTGAHSTSGNI---NGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 401 GNAGFGNSGLLNTGFGNAGST 421 GN G + +G G S Sbjct: 63 GNGGGNGNSGGGSGTGGNLSA 83
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_0894	UREASE	35	8e-04	Urea amidohydrolase (urease) protein signature.
>UREASE#Urea amidohydrolase (urease) protein signature. Length = 570 Score = 35.1 bits (81), Expect = 8e-04 Identities = 24/76 (31%), Positives = 35/76 (46%), Gaps = 9/76 (11%) Query: 6 DAIYTNGDIVTVDDEQPIAEA-VAVKDGRIVAVGAHD-----DVVRENLGPHTRRVDLAG 59 D + TN I+ D I +A + +KDGRI A+G V +GP T + G Sbjct: 69 DTVITNALIL---DHWGIVKADIGLKDGRIAAIGKAGNPDMQPGVTIIVGPGTEVIAGEG 125 Query: 60 NTLLPGFIDPHSHYIN 75 + G +D H H+I Sbjct: 126 KIVTAGGMDSHIHFIC 141 Score = 31.2 bits (71), Expect = 0.013 Identities = 13/30 (43%), Positives = 17/30 (56%) Query: 487 ITINAAYQYSEEQSKGSITVGKLADLVIVD 516 TIN A + GS+ VGK ADLV+ + Sbjct: 409 YTINPAIAHGLSHEIGSLEVGKRADLVLWN 438
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_0896	IGASERPTASE	30	0.017	IgA-specific serine endopeptidase (S6) signature.
>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature. Length = 1541 Score = 30.0 bits (67), Expect = 0.017 Identities = 17/51 (33%), Positives = 20/51 (39%), Gaps = 3/51 (5%) Query: 283 VTFDEDRHRAHTGNGAQVLATLRNTAINLHRLNGADNIAEACRITALTANR 333 V E+ H TGN L N I+ LN ADN + LT N Sbjct: 846 VRLTENSHWHLTGNSDVHQLDLANGHIH---LNSADNSNNVTKYNTLTVNS 893
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_0898	PF03544	30	0.011	Gram-negative bacterial tonB protein
>PF03544#Gram-negative bacterial tonB protein Length = 243 Score = 29.9 bits (67), Expect = 0.011 Identities = 22/104 (21%), Positives = 29/104 (27%), Gaps = 4/104 (3%) Query: 246 PAPSASPTTTGAKPSASLPPAGATATSPAPTSVPTPPVSAVVPGETPADTSVVAPGSPAA 305 PAP+ + T P+ PP A P P V P E P + VV Sbjct: 44 PAPAQPISVTMVAPADLEPPQ---AVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPK 100 Query: 306 AGVAAPGPAKLAAP-GDATNPGSPVVQTSGQPEPVEPAPAGPVS 348 K+ P D S P P + + Sbjct: 101 PKPKPKPVKKVEQPKRDVKPVESRPASPFENTAPARPTSSTATA 144
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_0899	CHLAMIDIAOMP	27	0.027	Chlamydia major outer membrane protein signature.
>CHLAMIDIAOMP#Chlamydia major outer membrane protein signature. Length = 393 Score = 26.9 bits (59), Expect = 0.027 Identities = 12/31 (38%), Positives = 13/31 (41%), Gaps = 11/31 (35%) Query: 5 LPPGLPPDP-----------FADDPCDPSAT 24 LP G P +P F DPCDP T Sbjct: 23 LPVGNPAEPSLMIDGILWEGFGGDPCDPCTT 53
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_0902	cloacin	30	0.033	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 30.1 bits (67), Expect = 0.033 Identities = 28/119 (23%), Positives = 38/119 (31%), Gaps = 13/119 (10%) Query: 413 GFGNGGNFNLGFGNGGAANVGVGNGGGGNLGFGNSGTENTGSFNSGGDNGRNGGNTGSFN 472 G G + G NGG +GVG G G+ + G SG G G+ Sbjct: 8 GHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGG 67 Query: 473 SGYVNTGFFNSGSTNTGLFNAGSVNTGIGSPDTQPGSISGFGNTGAGVSGFNNSGDATS 531 +G NSG + N +V + GAG + S A S Sbjct: 68 NG-------NSGGGSGTGGNLSAVAAPVAF------GFPALSTPGAGGLAVSISAGALS 113 Score = 30.1 bits (67), Expect = 0.033 Identities = 27/81 (33%), Positives = 36/81 (44%), Gaps = 12/81 (14%) Query: 396 NSGDTNT-GFWNAGRVNTGFGNGGNFNLGFG-----NGGAANVGVGNGGGGNLGFGNSGT 449 N+G +T G N G G G G + G+ GG + G+ GGG G GN G Sbjct: 10 NTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGS--GHGNGG- 66 Query: 450 ENTGSFNSGGDNGRNGGNTGS 470 G+ NSGG +G G + Sbjct: 67 ---GNGNSGGGSGTGGNLSAV 84 Score = 29.7 bits (66), Expect = 0.036 Identities = 30/84 (35%), Positives = 35/84 (41%), Gaps = 6/84 (7%) Query: 190 NTGVGNLSIGNI--GVFNLGGGNAGNLNLGGGNTGNANLGSGNNGFFNLGSGNTGNTNFG 247 NTG + S GNI G LG G + G + N G +G G GN G Sbjct: 10 NTGAHSTS-GNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGG-G 67 Query: 248 NGNRGNLNWGSGNLGN--ANVGFG 269 NGN G + GNL A V FG Sbjct: 68 NGNSGGGSGTGGNLSAVAAPVAFG 91 Score = 29.7 bits (66), Expect = 0.043 Identities = 24/77 (31%), Positives = 31/77 (40%), Gaps = 1/77 (1%) Query: 227 GSGNNGFFNLGSGNTGNTNFGNGNRGNLNWGSGNLGNANVGFGNFLGQGNFGFGNRVGDA 286 G G+N + SGN G G G + GSG + N +G G G G Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGGASDGSG-WSSENNPWGGGSGSGIHWGGGSGHGN 64 Query: 287 NLGSGNLGNANFGNGNL 303 G+GN G + GNL Sbjct: 65 GGGNGNSGGGSGTGGNL 81
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_0905	HTHTETR	60	1e-13	TetR bacterial regulatory protein HTH signature.
>HTHTETR#TetR bacterial regulatory protein HTH signature. Length = 215 Score = 60.4 bits (146), Expect = 1e-13 Identities = 25/97 (25%), Positives = 44/97 (45%), Gaps = 6/97 (6%) Query: 10 RKTPTGREEVAAAVLEAAADLFAERGPAATSIRDIAARSKVNHGLVFRHFGAKEQLVGAV 69 RKT +E +L+ A LF+++G ++TS+ +IA + V G ++ HF K L + Sbjct: 3 RKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSEI 62 Query: 70 LDYLGTHLTELL------RAGTPADDLERALDRQMRV 100 + +++ EL G P L L + Sbjct: 63 WELSESNIGELELEYQAKFPGDPLSVLREILIHVLES 99
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_0907	IGASERPTASE	30	0.016	IgA-specific serine endopeptidase (S6) signature.
>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature. Length = 1541 Score = 30.0 bits (67), Expect = 0.016 Identities = 17/51 (33%), Positives = 20/51 (39%), Gaps = 3/51 (5%) Query: 283 VTFDEDRHRAHTGNGAQVLATLRNTAINLHRLNGADNIAEACRITALTANR 333 V E+ H TGN L N I+ LN ADN + LT N Sbjct: 846 VRLTENSHWHLTGNSDVHQLDLANGHIH---LNSADNSNNVTKYNTLTVNS 893
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_0910	HTHTETR	61	9e-14	TetR bacterial regulatory protein HTH signature.
>HTHTETR#TetR bacterial regulatory protein HTH signature. Length = 215 Score = 61.2 bits (148), Expect = 9e-14 Identities = 33/181 (18%), Positives = 61/181 (33%), Gaps = 12/181 (6%) Query: 5 RTTARAGGDRRAAIIEAALNRFLSQGIDATALRQIQRDAGVSNGSFFHHFPSKEVLTAAV 64 R T + + R I++ AL F QG+ +T+L +I + AGV+ G+ + HF K L + + Sbjct: 3 RKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSEI 62 Query: 65 YVDCVTRYQQALLKDLV-RYPDAESAVRGIVGMHLSWCTEHPEMARFLITMTEPAVHRAA 123 + + + L+ D S +R I+ L + + Sbjct: 63 WELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVTEERRRLLMEIIFHKCE---F 119 Query: 124 ADELRSHNERFATAVQTWWRPHAHY-------GALRP-LSPAHSQALWLGPAQELVRAWL 175 E+ + + L L + + G L+ WL Sbjct: 120 VGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMTRRAAIIMRGYISGLMENWL 179 Query: 176 L 176 Sbjct: 180 F 180
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_0911	TCRTETB	157	1e-44	Tetracycline resistance protein TetB signature.
>TCRTETB#Tetracycline resistance protein TetB signature. Length = 458 Score = 157 bits (398), Expect = 1e-44 Identities = 96/404 (23%), Positives = 175/404 (43%), Gaps = 15/404 (3%) Query: 18 ICLSVFVISVDATIVNVALPTLSRELGADTAQLQWIVDAYTLVMSGLLLSAGSLSDRYGR 77 +C+ F ++ ++NV+LP ++ + A W+ A+ L S G LSD+ G Sbjct: 19 LCILSFFSVLNEMVLNVSLPDIANDFNKPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGI 78 Query: 78 RGWLSAGLILFALTSALAAQVNS-ADTLVAARAAMGVGAAVIFPTTLGLITNIFTDPVPR 136 + L G+I+ S + +S L+ AR G GAA FP + ++ + R Sbjct: 79 KRLLLFGIIINCFGSVIGFVGHSFFSLLIMARFIQGAGAAA-FPALVMVVVARYIPKENR 137 Query: 137 AKAIGLWTAMVGVGVAVGPITGGWLLEHFSWGSIFLVNVPIAVAAMAGAILFVPTSRDPA 196 KA GL ++V +G VGP GG + + W +L+ +P+ ++ + Sbjct: 138 GKAFGLIGSIVAMGEGVGPAIGGMIAHYIHWS--YLLLIPMITIITVPFLMKLLKKEVRI 195 Query: 197 APRVDVPGLMLSAVGITALVYTIIEAPNWGWTSTRASGGFIAAAIVLVGFALWERRSSHP 256 D+ G++L +VGI + +T++ + I + + + F R+ + P Sbjct: 196 KGHFDIKGIILMSVGIVFFML---------FTTSYSISFLIVSVLSFLIFVKHIRKVTDP 246 Query: 257 MLDVSVFANRRFSGGSLAVTAGFLTLFGFIFVITQYFQFIKDYTALQTG-VRLLPVAFSI 315 +D + N F G L F T+ GF+ ++ + + + + G V + P S+ Sbjct: 247 FVDPGLGKNIPFMIGVLCGGIIFGTVAGFVSMVPYMMKDVHQLSTAEIGSVIIFPGTMSV 306 Query: 316 ALAAILGPRLVERVGTTAVVAAGLAVFAAALAWASTADATTPYTQITLQMLLLGGGLGLT 375 + +G LV+R G V+ G+ + + AS TT + + + +LGG Sbjct: 307 IIFGYIGGILVDRRGPLYVLNIGVTFLSVSFLTASFLLETTSWFMTIIIVFVLGGLSFTK 366 Query: 376 TAPATEAIMGSLPADRAGVGSAVNDTTRELGGTLGVAIAGSVFA 419 T +T SL AG G ++ + T L G+AI G + + Sbjct: 367 TVISTIVSS-SLKQQEAGAGMSLLNFTSFLSEGTGIAIVGGLLS 409

MUL_0982

MUL_0990

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
MUL_0982	2	11	0.422076	hypothetical protein
MUL_0983	0	11	0.125430	PPE family protein
MUL_0985	0	12	0.313346	PPE family protein
MUL_0986	-1	14	0.260268	transposase for IS2606
MUL_0987	-1	14	-0.259408	PE family protein
MUL_0988	-1	14	-0.253420	acetyl-CoA acetyltransferase
MUL_0989	0	12	-0.391459	enoyl-CoA hydratase, EchA1
MUL_0990	1	15	-0.775833	transposase for IS2606

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_0982	NUCEPIMERASE	63	3e-13	Nucleotide sugar epimerase signature.
>NUCEPIMERASE#Nucleotide sugar epimerase signature. Length = 334 Score = 63.3 bits (154), Expect = 3e-13 Identities = 31/125 (24%), Positives = 51/125 (40%), Gaps = 18/125 (14%) Query: 38 MRILVTGATGYVGSRLVTALLADGHEVLA---------ATRNMARLSRLAWFDDVTPVIL 88 M+ LVTGA G++G + LL GH+V+ + ARL LA + Sbjct: 1 MKYLVTGAAGFIGFHVSKRLLEAGHQVVGIDNLNDYYDVSLKQARLELLA-QPGFQFHKI 59 Query: 89 DATDRASAQAAMNAAGQIDVVYYLVH------GIGQPD-FRDRDKTAAANLAVAARDTGV 141 D DR + A+G + V+ H + P + D + T N+ R + Sbjct: 60 DLADRE-GMTDLFASGHFERVFISPHRLAVRYSLENPHAYADSNLTGFLNILEGCRHNKI 118 Query: 142 RRIVY 146 + ++Y Sbjct: 119 QHLLY 123
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_0987	DHBDHDRGNASE	72	3e-17	2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase signature. Length = 261 Score = 72.4 bits (177), Expect = 3e-17 Identities = 59/205 (28%), Positives = 88/205 (42%), Gaps = 19/205 (9%) Query: 3 IRDAVAVVTGGASGLGLATTKRLLDAGAQVVVLDIRGE---DVVADLGDRARFA---AAD 56 I +A +TG A G+G A + L GA + +D E VV+ L AR A AD Sbjct: 6 IEGKIAFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHAEAFPAD 65 Query: 57 VTDEAAVASALD-LAETMGTLRIVVNCAGTGNAIRVLSRDGVFSLAAFRKIVDINLVGSF 115 V D AA+ + MG + I+VN AG +R + S + +N G F Sbjct: 66 VRDSAAIDEITARIEREMGPIDILVNVAGV---LRPGLIHSL-SDEEWEATFSVNSTGVF 121 Query: 116 NVLRLAAERIAKTEPVGPNAEERGVIINTASVAAFDGQIGQAAYSASKGGVVGMTLPIAR 175 N R ++ + G I+ S A + AAY++SK V T + Sbjct: 122 NASRSVSKYM--------MDRRSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGL 173 Query: 176 DLASHRIRVMTIAPGLFDTPLLASL 200 +LA + IR ++PG +T + SL Sbjct: 174 ELAEYNIRCNIVSPGSTETDMQWSL 198
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_0989	ACRIFLAVINRP	54	2e-09	Acriflavin resistance protein family signature.
>ACRIFLAVINRP#Acriflavin resistance protein family signature. Length = 1034 Score = 53.7 bits (129), Expect = 2e-09 Identities = 36/233 (15%), Positives = 85/233 (36%), Gaps = 29/233 (12%) Query: 186 LIAIPLSFLVLVWVFGGLLAAALPMALGALAVVGSMSVLRLVTFTTDVSIFALNLSTALG 245 AI L FLV+ + A +P + ++G+ ++L ++ +N T G Sbjct: 345 FEAIMLVFLVMYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYS-------INTLTMFG 397 Query: 246 LALAI-----DYTLLIISRYRDELAEGSSREEALVRTMATSGRTVLFSAVT---VALSMS 297 + LAI D +++ + R + + +EA ++M+ ++ A+ V + M+ Sbjct: 398 MVLAIGLLVDDAIVVVENVERVMMEDKLPPKEATEKSMSQIQGALVGIAMVLSAVFIPMA 457 Query: 298 ATVAFPMYFLKSFAYAGVATVAFVATASIVVTPAAIVLLGPRLDALNVRRLARRMLGRPK 357 + F+ V+ +A ++++TPA L ++ ++ Sbjct: 458 FFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATL--------LKPVSAEHHENKG 509 Query: 358 PQHKPVDQLF------WYRSTKFVMRRALPVGLAVVAVLVILGLPFFSVKWGF 404 + F + S ++ L ++ + + F + F Sbjct: 510 GFFGWFNTTFDHSVNHYTNSVGKILGSTGRYLLIYALIVAGMVVLFLRLPSSF 562 Score = 40.6 bits (95), Expect = 3e-05 Identities = 42/233 (18%), Positives = 81/233 (34%), Gaps = 27/233 (11%) Query: 116 SAPDLVSKDGKSGL-IVVNIKGGES--NAQKNAQTLADEIVHDRDGVTVRAGGSAMEYAQ 172 +P L +G + I G S +A + LA ++ G+ G + + Sbjct: 811 GSPRLERYNGLPSMEIQGEAAPGTSSGDAMALMENLASKLP---AGIGYDWTGMSYQERL 867 Query: 173 INKQNQDDLLVMELIAIPLSFLVLVWVFGGLLAAALPMALGALAVVGSMSVLRLVTFTTD 232 Q + I+ + FL L ++ M + L +VG + L D Sbjct: 868 SGNQ----APALVAISFVVVFLCLAALYESWSIPVSVMLVVPLGIVGVLLAATLFNQKND 923 Query: 233 VSIFALNLSTALGLALAIDYTLLIISRYRDEL-AEGSSREEALVRTMATSGRTVLFSAVT 291 V F + L T +G L+ +LI+ +D + EG EA + + R +L +++ Sbjct: 924 V-YFMVGLLTTIG--LSAKNAILIVEFAKDLMEKEGKGVVEATLMAVRMRLRPILMTSLA 980 Query: 292 VALSMSATVAFPMYF--------LKSFAYAGVATVAFVATASIVVTPAAIVLL 336 L + P+ + + + +I P V++ Sbjct: 981 FILGV-----LPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIFFVPVFFVVI 1028
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_0990	HTHTETR	67	1e-15	TetR bacterial regulatory protein HTH signature.
>HTHTETR#TetR bacterial regulatory protein HTH signature. Length = 215 Score = 66.6 bits (162), Expect = 1e-15 Identities = 35/164 (21%), Positives = 61/164 (37%), Gaps = 11/164 (6%) Query: 4 ARAPRGSGDLLRHEILDAATELLLQTRQARAVSIRSVAERVGVTSPSIYLHFQDKDALLD 63 AR + R ILD A L Q + + S+ +A+ GVT +IY HF+DK L Sbjct: 2 ARKTKQEAQETRQHILDVALRLFSQ-QGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFS 60 Query: 64 AVCARYLARLDE-EMERAAMGHTCVVEVLRAQGLAYVRFALQTPELYRLATM-------- 114 + + + E E+E A + VLR + + + L + Sbjct: 61 EIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVTEERRRLLMEIIFHKCEFV 120 Query: 115 GEWRSGSNVDSALDSSAFRHMCASVQAMMDEGIYRAD-DPTTIA 157 GE L ++ + +++ ++ + AD A Sbjct: 121 GEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMTRRAA 164

MUL_1004

MUL_1010

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
MUL_1004	-1	12	3.246056	hypothetical protein
MUL_1005	-2	13	1.915889	NAD-dependent deacetylase
MUL_1006	-1	10	0.267671	transcriptional regulatory protein
MUL_1007	-2	8	-0.585443	hypothetical protein
MUL_1008	-2	9	0.008310	hypothetical protein
MUL_1009	-1	9	0.013961	hypothetical protein
MUL_1010	-1	9	-0.361376	non-IS element not present in Mycobacterium

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_1004	PF03544	33	0.001	Gram-negative bacterial tonB protein
>PF03544#Gram-negative bacterial tonB protein Length = 243 Score = 33.4 bits (76), Expect = 0.001 Identities = 29/119 (24%), Positives = 35/119 (29%), Gaps = 1/119 (0%) Query: 155 HAAGPAPVAAPAGAPPASAPAPAAAAPASAPGTAPAPAAAPGPAPAAPAPAP-AAAAPAP 213 PAP + A A A P P P P P P AP P P Sbjct: 40 VIELPAPAQPISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKP 99 Query: 214 APAPAAPPAAPVAAPVAAPAPVPAPAPAAAPAPEAAAPAPAPAPAPAAAPGFGPDAPPT 272 P P P V P PV + + A P + A A + P + P Sbjct: 100 KPKPKPKPVKKVEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPR 158 Score = 31.1 bits (70), Expect = 0.006 Identities = 30/132 (22%), Positives = 38/132 (28%), Gaps = 8/132 (6%) Query: 131 VPHVPAPGAEPGTLAHLPAGIDPAHA--------AGPAPVAAPAGAPPASAPAPAAAAPA 182 V +PAP PA ++P A P P P PP AP Sbjct: 40 VIELPAPAQPISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKP 99 Query: 183 SAPGTAPAPAAAPGPAPAAPAPAPAAAAPAPAPAPAAPPAAPVAAPVAAPAPVPAPAPAA 242 P A+P APA P ++ A + P A P A Sbjct: 100 KPKPKPKPVKKVEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRA 159 Query: 243 APAPEAAAPAPA 254 + PA A Sbjct: 160 LSRNQPQYPARA 171
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_1006	PF06580	29	0.042	Sensor histidine kinase
>PF06580#Sensor histidine kinase Length = 349 Score = 28.7 bits (64), Expect = 0.042 Identities = 17/87 (19%), Positives = 28/87 (32%), Gaps = 18/87 (20%) Query: 343 LVPLMIWVLSGPLRDRLGARILGW---------GWLALTVIGVPWLLSFAQPTIWQI--- 390 + LM VL+ R + + A VIG+ W A +IW++ Sbjct: 47 AISLMGLVLTHAYRSFIKRQGWLKLNMGQIILRVLPACVVIGMVWF--VANTSIWRLLAF 104 Query: 391 ----GRPWYLAWAGLVYVVATLATLGW 413 + L A + + T W Sbjct: 105 INTKPVAFTLPLALSIIFNVVVVTFMW 131
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_1009	TCRTETOQM	186	2e-53	Tetracycline resistance protein TetO/TetQ/TetM family ...
>TCRTETOQM#Tetracycline resistance protein TetO/TetQ/TetM family signature. Length = 639 Score = 186 bits (475), Expect = 2e-53 Identities = 105/447 (23%), Positives = 173/447 (38%), Gaps = 62/447 (13%) Query: 1 MLFRNVAIVAHVDHGKTTLVDAMLRQSGALTERGEVQE--RVMDSGDLEREKGITILAKN 58 M N+ ++AHVD GKTTL +++L SGA+TE G V + D+ LER++GITI Sbjct: 1 MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTDNTLLERQRGITIQTGI 60 Query: 59 TAVHRHHPDGTVTVINVIDTPGHADFGGEVERGLSMVDGVLLLVDASEGPLPQTGFVLRK 118 T+ + T +N+IDTPGH DF EV R LS++DG +LL+ A +G QT + Sbjct: 61 TSFQWEN-----TKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAKDGVQAQTRILFHA 115 Query: 119 ALSAHLPVILVVNKTDRPDARIKEVVEASHDLLLDVA----------------SDLDDEA 162 +P I +NK D+ + V + + L ++ Sbjct: 116 LRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVELYPNMCVTNFTESEQW 175 Query: 163 AAAAEHALGLPTLYASG------------RAGIAS-TIEPA-DGQAPDGTNLDPLFDVLL 208 E L Y SG + ++ P G A + +D L +V+ Sbjct: 176 DTVIEGNDDLLEKYMSGKSLEALELEQEESIRFHNCSLFPVYHGSAKNNIGIDNLIEVIT 235 Query: 209 EHVPPPQGDSEAPLQALVTNLDASAFLGRLALVRIYNGKLRKGQQVAWLREVDGVPVVTS 268 ++ L V ++ S RLA +R+Y+G L V + Sbjct: 236 NKFYSSTHRGQSELCGKVFKIEYSEKRQRLAYIRLYSGVLHLRDSVR-------ISEKEK 288 Query: 269 AKITELLATEGVERSTTDEAVAGDIVAVAGLP---EIMIGDTLADPDHAHALPRITVDEP 325 KITE+ + E D+A +G+IV + ++GDT P I P Sbjct: 289 IKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLPQRER----IENPLP 344 Query: 326 AISVTVGTNTSPLAGKVSGHKLTARMVRGRLDTELIGNVSIRVVDIGRPDAWEVQGRGEL 385 + TV + + L + +R + G++ Sbjct: 345 LLQTTVEPSKPQQREM----------LLDALLEISDSDPLLRYYVDSATHEIILSFLGKV 394 Query: 386 ALAVLVETMRRE-GFELTVGKPQVVTK 411 + V ++ + E+ + +P V+ Sbjct: 395 QMEVTCALLQEKYHVEIEIKEPTVIYM 421 Score = 43.3 bits (102), Expect = 3e-06 Identities = 18/84 (21%), Positives = 32/84 (38%), Gaps = 1/84 (1%) Query: 416 QLHEPFEAMTIDCPDEFVGAITQLMAGRKGRVEEMTNHAAGWVRMDFIVPSRGLIGFRTD 475 +L EP+ + I P E++ + + T V + +P+R + +R+D Sbjct: 534 ELLEPYLSFKIYAPQEYLSRAYTDAPKYCANIVD-TQLKNNEVILSGEIPARCIQEYRSD 592 Query: 476 FLTLTRGTGIANAVFDGYRPWAGE 499 T G + GY GE Sbjct: 593 LTFFTNGRSVCLTELKGYHVTTGE 616
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_1010	RTXTOXINA	31	0.021	Gram-negative bacterial RTX toxin determinant A family...
>RTXTOXINA#Gram-negative bacterial RTX toxin determinant A family signature. Length = 1024 Score = 30.7 bits (69), Expect = 0.021 Identities = 32/129 (24%), Positives = 50/129 (38%), Gaps = 21/129 (16%) Query: 413 GGISKDGRQLTLVIGVAANDPTSVAVANTAADQLRNVGIAASV--LALDPVTLYGDALND 470 G + K Q + A TS A A G+ AS LA+ P++ A Sbjct: 281 GNVGKGISQYIIAQRAAQGLSTSAAAA----------GLIASAVTLAISPLSFLSIADKF 330 Query: 471 NRVDAIVGWHQA----GGNLATLLASRY---GC--PALQTTEVWEPTIPANTPAATTGSM 521 R + I + Q G + +LLA+ + G +L T ++ + AA T S+ Sbjct: 331 KRANKIEEYSQRFKKLGYDGDSLLAAFHKETGAIDASLTTISTVLASVSSGISAAATTSL 390 Query: 522 PSAVPSAVT 530 A SA+ Sbjct: 391 VGAPVSALV 399

MUL_1034

MUL_1039

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
MUL_1034	-1	12	1.331461	GTP-binding translation elongation factor TypA
MUL_1035	-1	13	1.729203	lipoprotein LpqW
MUL_1037	-1	12	1.511925	transposase for IS2404
MUL_1038	-1	10	1.858258	transposase for IS2606
MUL_1039	-1	12	1.535475	N-acetyl-1-D-myo-inosityl-2-amino-2-deoxy-alpha-

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_1034	RTXTOXINA	32	0.006	Gram-negative bacterial RTX toxin determinant A family...
>RTXTOXINA#Gram-negative bacterial RTX toxin determinant A family signature. Length = 1024 Score = 32.2 bits (73), Expect = 0.006 Identities = 25/118 (21%), Positives = 42/118 (35%), Gaps = 22/118 (18%) Query: 20 GIAATLQVANSAAAALTSGLLAAAQDEVSAAIA-------------KVFSAYGQEYQAAI 66 A L + +AA + S + A +IA + F G + + + Sbjct: 295 RAAQGLSTSAAAAGLIASAVTLAISPLSFLSIADKFKRANKIEEYSQRFKKLGYDGDSLL 354 Query: 67 AQ-------ASAFHTEFTRALAAAGAAYAQAEAANASLITAGVSDALTAITTPIQSLL 117 A A T + LA+ + + A SL+ A VS + A+T I +L Sbjct: 355 AAFHKETGAIDASLTTISTVLASVSSGISAAATT--SLVGAPVSALVGAVTGIISGIL 410
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_1037	DHBDHDRGNASE	125	2e-37	2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase signature. Length = 261 Score = 125 bits (316), Expect = 2e-37 Identities = 75/251 (29%), Positives = 113/251 (45%), Gaps = 18/251 (7%) Query: 4 VDGKVALISGGARGMGASHARLLVQEGAKVVIGDILDEEGKALAEEIGDAARYVH---LD 60 ++GK+A I+G A+G+G + AR L +GA + D E+ + + + AR+ D Sbjct: 6 IEGKIAFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHAEAFPAD 65 Query: 61 VTQPDQWEAAVATAVDEFGKLDVLVNNVGIVALGQLKKFDLGKWQKVIDVNLTGTFLGMR 120 V + A E G +D+LVN G++ G + +W+ VN TG F R Sbjct: 66 VRDSAAIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGVFNASR 125 Query: 121 AAVEPMTAAGSGSIINVSSIEGLRGAPAVHPYVASKWAVRGLTKSAALELAPLNIRVNSI 180 + + M SGSI+ V S ++ Y +SK A TK LELA NIR N + Sbjct: 126 SVSKYMMDRRSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLELAEYNIRCNIV 185 Query: 181 HPGFIRTPMTANLPDD---------------MVTIPLGRPAESREVSTFVVFLASDDASY 225 PG T M +L D IPL + A+ +++ V+FL S A + Sbjct: 186 SPGSTETDMQWSLWADENGAEQVIKGSLETFKTGIPLKKLAKPSDIADAVLFLVSGQAGH 245 Query: 226 ATGSEFVMDGG 236 T +DGG Sbjct: 246 ITMHNLCVDGG 256
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_1038	PF00577	30	0.028	Outer membrane usher protein FimD
>PF00577#Outer membrane usher protein FimD Length = 878 Score = 29.8 bits (67), Expect = 0.028 Identities = 28/159 (17%), Positives = 46/159 (28%), Gaps = 26/159 (16%) Query: 30 GRYRGRASALVRPASADQVAEVLRVCRDAGAHVTVQGGRTSLVAGTVP-EHDDVLLSTER 88 G+ LV A + +V G +G +++ + V L T Sbjct: 711 GQPLNDTVVLV---KAPGAKDA-KVENQTGVRTDWRG--YAVLPYATEYRENRVALDTNT 764 Query: 89 ICDVADVDTLERRVAVGAGA---------TLAAVQRAATAAG--LVFGVDLSARESATVG 137 + D D+D V GA + T L FG +++ S + G Sbjct: 765 LADNVDLDNAVANVVPTRGAIVRAEFKARVGIKLLMTLTHNNKPLPFGAMVTSESSQSSG 824 Query: 138 -----GMA---STNAGGLRTVRYGNMGEQVVGLDVALPD 168 G G V++G + LP Sbjct: 825 IVADNGQVYLSGMPLAGKVQVKWGEEENAHCVANYQLPP 863
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_1039	HTHTETR	55	7e-12	TetR bacterial regulatory protein HTH signature.
>HTHTETR#TetR bacterial regulatory protein HTH signature. Length = 215 Score = 55.4 bits (133), Expect = 7e-12 Identities = 23/95 (24%), Positives = 38/95 (40%), Gaps = 2/95 (2%) Query: 18 RQRILAATAEVLARSGKTKLSLSEVAAQAGVSRPTLYRWFASKEELL-ATFSRYERQVFE 76 RQ IL + ++ G + SL E+A AGV+R +Y F K +L + E + E Sbjct: 13 RQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSEIWELSESNIGE 72 Query: 77 SGLSKATAGLKG-VDKLDAALRFIVDYQYSYSGVR 110 L + L L +++ + R Sbjct: 73 LELEYQAKFPGDPLSVLREILIHVLESTVTEERRR 107

MUL_1084

MUL_1092

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
MUL_1084	-1	11	0.929047	MCE family lipoprotein LprK
MUL_1085	-1	12	0.727138	MCE-family protein Mce1F
MUL_1086	-1	12	1.357599	Mce associated membrane protein
MUL_1088	-1	12	1.658678	Mce associated transmembrane protein
MUL_1089	0	13	2.061293	Mce associated protein
MUL_1090	0	13	2.001402	Mce associated membrane protein
MUL_1091	0	12	1.928261	transmembrane protein
MUL_1092	1	11	1.819964	hypothetical protein

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_1084	TCRTETA	44	9e-07	Tetracycline resistance protein signature.
>TCRTETA#Tetracycline resistance protein signature. Length = 399 Score = 43.7 bits (103), Expect = 9e-07 Identities = 77/356 (21%), Positives = 127/356 (35%), Gaps = 28/356 (7%) Query: 38 ILPVGALSAIARNLHISVVLV---GTLLSWYALVAALTTVPLVRWTAHWPRRHALMVSLV 94 I+PV L + R+L S + G LL+ YAL+ L + + RR L+VSL Sbjct: 24 IMPV--LPGLLRDLVHSNDVTAHYGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLA 81 Query: 95 CLTISQLISALAPNFAVLAAGRALCAITHG---LLWSVIAPIATRLVPPSHAGRATTSIY 151 + I A AP VL GR + IT + + IA I H G + Sbjct: 82 GAAVDYAIMATAPFLWVLYIGRIVAGITGATGAVAGAYIADITDGDERARHFGFMSACFG 141 Query: 152 IGTSLALVIGSPLTAALSLMWGWRLAAVCVTVAAAVVTVAARLLLPEMVLSAD---QLQH 208 G V+G L S + AA + LLPE + + Sbjct: 142 FGMVAGPVLGG-LMGGFSPHAPFFAAAALNGLNFLTGC----FLLPESHKGERRPLRREA 196 Query: 209 VGPRSRHHRNRALIIVSLITMVGVTGHFVSY---TYIVVIIRQVVGVRGPSLAWLLAAYG 265 + P + R + +V+ + V V V+ ++ LAA+G Sbjct: 197 LNPLASFRWARGMTVVAALMAVFFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFG 256 Query: 266 VAGVVAVALVARPLDRRPKGTIIFCVAGLTFAFVLLTALAFGGHLAPMTALVVGTGAIVL 325 + +A A++ P+ R G + G+ LAF M ++ ++L Sbjct: 257 ILHSLAQAMITGPVAAR-LGERRALMLGMIADGTGYILLAFATR-GWMAFPIM----VLL 310 Query: 326 WGAAVTAVSPMLQSAAMRSGADDPDGASGLYVTAFQ-VGIMAGSLIGGLLYERSVA 380 + P LQ+ R ++ G + A + + G L+ +Y S+ Sbjct: 311 ASGGIG--MPALQAMLSRQVDEERQGQLQGSLAALTSLTSIVGPLLFTAIYAASIT 364
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_1085	PF03544	31	0.005	Gram-negative bacterial tonB protein
>PF03544#Gram-negative bacterial tonB protein Length = 243 Score = 31.1 bits (70), Expect = 0.005 Identities = 15/76 (19%), Positives = 19/76 (25%), Gaps = 1/76 (1%) Query: 32 PAVADPDDAPGDPPVIAPAEPAPDPLAPPPGPLALPPMPDPLAPPPLVPVAAGPVAGQDP 91 P P P P EP P+P P + P P P+ V + Sbjct: 63 PQAVQPPPEPVVEPEP-EPEPIPEPPKEAPVVIEKPKPKPKPKPKPVKKVEQPKRDVKPV 121 Query: 92 TPFFGPPPFRPPSFNP 107 P P Sbjct: 122 ESRPASPFENTAPARP 137
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_1086	HTHTETR	35	1e-04	TetR bacterial regulatory protein HTH signature.
>HTHTETR#TetR bacterial regulatory protein HTH signature. Length = 215 Score = 34.6 bits (79), Expect = 1e-04 Identities = 17/146 (11%), Positives = 48/146 (32%), Gaps = 14/146 (9%) Query: 4 SAVLLIRERGACATAISDVLQHSGAPRGSAYHYFPGGRTQLLCEAVDYAGAHVAKIIGSA 63 A+ L ++G +T++ ++ + +G RG+ Y +F ++ L E + + +++ ++ Sbjct: 19 VALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHF-KDKSDLFSEIWELSESNIGELE--L 75 Query: 64 TRSLDLLDTLIDQYREQLRATDFRAGCPVAAVSVEAGEPSDRERMAPVVEHAAAVFDRWS 123 + RE L + R + ++ H + Sbjct: 76 EYQAKFPGDPLSVLREILIHV-LES----------TVTEERRRLLMEIIFHKCEFVGEMA 124 Query: 124 DLIAQRFVSDGIPLDSAHELAVTAMS 149 + + D + + Sbjct: 125 VVQQAQRNLCLESYDRIEQTLKHCIE 150
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_1092	ACRIFLAVINRP	65	1e-12	Acriflavin resistance protein family signature.
>ACRIFLAVINRP#Acriflavin resistance protein family signature. Length = 1034 Score = 64.9 bits (158), Expect = 1e-12 Identities = 41/227 (18%), Positives = 90/227 (39%), Gaps = 28/227 (12%) Query: 188 IILIVLLAVFGSLTAAAI-ALALGICTVVVTMGLVYLLSMHTTMSVFVTSTVSMFGIALA 246 ++ +V+ ++ A I +A+ + ++ T ++ + +T++MFG+ LA Sbjct: 350 LVFLVMYLFLQNMRATLIPTIAVPV-VLLGTFAILAAFG-------YSINTLTMFGMVLA 401 Query: 247 ----VDYSLFILMRFREELRSGR-QPQEAADAAMATSGLAVVLSGMTVVASLSGIYLINT 301 VD ++ ++ + + P+EA + +M+ A+V M + A + Sbjct: 402 IGLLVDDAIVVVENVERVMMEDKLPPKEATEKSMSQIQGALVGIAMVLSAVFIPMAFFGG 461 Query: 302 PA---LRSMATGAILAVAVAILTSATLTPAVLATFARAAAK-----RSALLHWSRRPEST 353 R + + A+A+++L + LTPA+ AT + + + W Sbjct: 462 STGAIYRQFSITIVSAMALSVLVALILTPALCATLLKPVSAEHHENKGGFFGWFNTTFDH 521 Query: 354 QSRFWTRWVGWVMHRPWITALSASVVLLLMAAPAASMVLGNSLLRQF 400 +T VG ++ L ++++ M VL L F Sbjct: 522 SVNHYTNSVGKILGSTGRYLLIYALIVAGMV------VLFLRLPSSF 562

MUL_1226

MUL_1242

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
MUL_1226	-2	11	0.089173	hypothetical protein
MUL_1227	-2	8	0.562190	PE family protein
MUL_1231	-1	11	-0.480607	PPE family protein
MUL_1233	-1	6	0.126662	EsaT-6 like protein EsxG
MUL_1234	0	8	1.073007	EsaT-6 like protein EsxH
MUL_1236	0	7	1.184629	hypothetical protein
MUL_1237	0	11	1.741383	transmembrane protein
MUL_1238	0	15	0.962464	membrane-anchored mycosin MycP3
MUL_1239	0	15	0.255501	transmembrane protein
MUL_1240	-1	16	-0.524744	hypothetical protein
MUL_1241	-1	12	-0.575917	trans-aconitate 2-methyltransferase
MUL_1242	0	13	-0.616408	sulfatase

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_1226	PF03544	41	5e-06	Gram-negative bacterial tonB protein
>PF03544#Gram-negative bacterial tonB protein Length = 243 Score = 41.1 bits (96), Expect = 5e-06 Identities = 28/125 (22%), Positives = 40/125 (32%), Gaps = 3/125 (2%) Query: 470 VVPSVGPLPAPSRSIAASSAPPKALEP--SFVPPPASAAR-APSAAPAPTSVAPPPPPPR 526 V V LPAP++ I+ + P LEP + PPP P P P P Sbjct: 36 SVHQVIELPAPAQPISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIE 95 Query: 527 PVATATTTVAPPVTTTKTTVPPTTAATTTPTTTPPPTTTAPPTTTAPPSTTSTVKMTTEW 586 PV + + P + T A PT++ + TS + Sbjct: 96 KPKPKPKPKPKPVKKVEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVAS 155 Query: 587 LHVPL 591 L Sbjct: 156 GPRAL 160 Score = 29.6 bits (66), Expect = 0.030 Identities = 21/102 (20%), Positives = 29/102 (28%), Gaps = 3/102 (2%) Query: 467 QSPVVPSVGPLPAPSRSIAASSAPPKALEPSFVPPPASAARAPSAAPAPTSVAPPPPPPR 526 Q P P V P P P P +E P P + P Sbjct: 67 QPPPEPVVEPEPEPEPIPEPPKEAPVVIEK---PKPKPKPKPKPVKKVEQPKRDVKPVES 123 Query: 527 PVATATTTVAPPVTTTKTTVPPTTAATTTPTTTPPPTTTAPP 568 A+ AP T+ T T+ T+ + P + P Sbjct: 124 RPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQP 165
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_1227	PF03544	37	1e-04	Gram-negative bacterial tonB protein
>PF03544#Gram-negative bacterial tonB protein Length = 243 Score = 36.9 bits (85), Expect = 1e-04 Identities = 33/166 (19%), Positives = 44/166 (26%), Gaps = 13/166 (7%) Query: 280 GRRRPLVLAGSAMLGIAAFAAGLMVVTLTSDVRPAAATQPSPREGVLTPASPAAPPKTAT 339 RR P S + A A L PA PA P + A Sbjct: 11 PRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPA-------------PAQPISVTMVAP 57 Query: 340 PVPAKAPAQVPAPGPAPAASPVPVAAPPSVVQPPPVVRAPRPTVVKPPPQYIAPATQPRR 399 A P P P P P P + P V+ P+P P R Sbjct: 58 ADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKPVKKVEQPKRD 117 Query: 400 TPAPQAPAPTPVQAPAPEVPPPVAVPEPAPAPPVPALVVERLVIQN 445 ++ +P + AP P P R + +N Sbjct: 118 VKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRN 163 Score = 31.5 bits (71), Expect = 0.005 Identities = 22/83 (26%), Positives = 28/83 (33%), Gaps = 7/83 (8%) Query: 364 AAPPSVVQPPPVVRAPRPTVVKPPPQYIAPATQPRRTPAPQAPAPTPVQAPAPEVPPPVA 423 A P SV P P V PP + P +P P P AP + P Sbjct: 47 AQPISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAP-------VVIEKPKP 99 Query: 424 VPEPAPAPPVPALVVERLVIQND 446 P+P P P +R V + Sbjct: 100 KPKPKPKPVKKVEQPKRDVKPVE 122 Score = 31.5 bits (71), Expect = 0.006 Identities = 22/87 (25%), Positives = 32/87 (36%), Gaps = 2/87 (2%) Query: 368 SVVQPPPVVRAPRP-TVVKPPPQYIAPATQPRRTPAPQA-PAPTPVQAPAPEVPPPVAVP 425 SV Q + +P +V P + P + P P P P P P P PV + Sbjct: 36 SVHQVIELPAPAQPISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIE 95 Query: 426 EPAPAPPVPALVVERLVIQNDGVDLAE 452 +P P P V+++ V E Sbjct: 96 KPKPKPKPKPKPVKKVEQPKRDVKPVE 122
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_1234	SECYTRNLCASE	28	0.033	Preprotein translocase SecY subunit signature.
>SECYTRNLCASE#Preprotein translocase SecY subunit signature. Length = 437 Score = 27.8 bits (62), Expect = 0.033 Identities = 11/40 (27%), Positives = 18/40 (45%), Gaps = 1/40 (2%) Query: 150 AVMPLIPYLLGFG-SLSAGLIFGGAGLLIAGGVTARFTRK 188 ++ L+P + G S FGG +LI GV ++ Sbjct: 383 GLIALVPTMALVGFGASQNFPFGGTSILIIVGVGLETVKQ 422
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_1236	IGASERPTASE	30	0.017	IgA-specific serine endopeptidase (S6) signature.
>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature. Length = 1541 Score = 30.0 bits (67), Expect = 0.017 Identities = 17/51 (33%), Positives = 20/51 (39%), Gaps = 3/51 (5%) Query: 283 VTFDEDRHRAHTGNGAQVLATLRNTAINLHRLNGADNIAEACRITALTANR 333 V E+ H TGN L N I+ LN ADN + LT N Sbjct: 846 VRLTENSHWHLTGNSDVHQLDLANGHIH---LNSADNSNNVTKYNTLTVNS 893
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_1237	PF03544	35	6e-04	Gram-negative bacterial tonB protein
>PF03544#Gram-negative bacterial tonB protein Length = 243 Score = 35.3 bits (81), Expect = 6e-04 Identities = 20/112 (17%), Positives = 32/112 (28%), Gaps = 3/112 (2%) Query: 125 PAPPQPVPPSKPTESVTQQLPTASAT-QRIATP--PPAPSTFERATRPIRLAPLGAPAAP 181 PAP QP+ + + + + + P P P P+ + P Sbjct: 44 PAPAQPISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKP 103 Query: 182 PPMPPHPPAPPSPPRPPSQSSPTPPTPELPAASAPAASEGEEQPKSRGLVER 233 P P P P +S P P A +++ K V Sbjct: 104 KPKPVKKVEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVAS 155 Score = 31.9 bits (72), Expect = 0.008 Identities = 20/104 (19%), Positives = 28/104 (26%), Gaps = 2/104 (1%) Query: 103 RSLPADPPRGPGHPAPRPARAEPAPPQPVPPSKPTESVTQQLPTASATQRIATPPPAPST 162 + P P P P + AP + KP + P Q P S Sbjct: 67 QPPPEPVVEPEPEPEPIPEPPKEAPVV-IEKPKPKP-KPKPKPVKKVEQPKRDVKPVESR 124 Query: 163 FERATRPIRLAPLGAPAAPPPMPPHPPAPPSPPRPPSQSSPTPP 206 A + A + S PR S++ P P Sbjct: 125 PASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQPQYP 168 Score = 31.9 bits (72), Expect = 0.009 Identities = 23/131 (17%), Positives = 41/131 (31%), Gaps = 9/131 (6%) Query: 147 ASATQRIATPPPAPSTFERATRPIRLAPLGAPA-APPPMPPHPPAPPSPPRPPSQSSPTP 205 S Q I P PA +PI + + PP PP P P P + P P Sbjct: 35 TSVHQVIELPAPA--------QPISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEP 86 Query: 206 PTPELPAASAPAASEGEEQPKSRGLVERMIDATRKLLPDRAETDSASASDSDSDSGSSTG 265 P P + + + + D + ++ + + S + ++ Sbjct: 87 PKEAPVVIEKPKPKPKPKPKPVKKVEQPKRDVKPVESRPASPFENTAPARPTSSTATAAT 146 Query: 266 TGELPSTNRLP 276 + + S P Sbjct: 147 SKPVTSVASGP 157 Score = 29.6 bits (66), Expect = 0.041 Identities = 17/116 (14%), Positives = 27/116 (23%) Query: 103 RSLPADPPRGPGHPAPRPARAEPAPPQPVPPSKPTESVTQQLPTASATQRIATPPPAPST 162 P + PA EP PP E + P + P Sbjct: 40 VIELPAPAQPISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKP 99 Query: 163 FERATRPIRLAPLGAPAAPPPMPPHPPAPPSPPRPPSQSSPTPPTPELPAASAPAA 218 + P+ P +P P +S T ++ A+ Sbjct: 100 KPKPKPKPVKKVEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVAS 155
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_1242	ARGDEIMINASE	36	1e-04	Bacterial arginine deiminase signature.
>ARGDEIMINASE#Bacterial arginine deiminase signature. Length = 409 Score = 36.4 bits (84), Expect = 1e-04 Identities = 38/187 (20%), Positives = 66/187 (35%), Gaps = 42/187 (22%) Query: 142 EGQGDLLRVGEMVLA-GYGFRTDPRSHAEIAAALRMPVISLELV-------DPRFYHLDT 193 EG GD L + + +L G RT+ +S ++A +L S + + + + HLDT Sbjct: 217 EG-GDELVLNKGLLVIGISERTEAKSVEKLAISLFKNKTSFDTILAFQIPKNRSYMHLDT 275 Query: 194 VLAVLDDHTIAYYP------------PAFSAAA----------RDQLQALF---PDAILV 228 V +D + S++ +D L D I Sbjct: 276 VFTQIDYSVFTSFTSDDMYFSIYVLTYNPSSSKIHIKKEKARIKDVLSFYLGRKIDIIKC 335 Query: 229 SSADAYAFGLNAVSDGCNVVI--PAAATGFALQ------LSEAGFEPIGVDLSELLKGGG 280 + D +DG NV+ P ++ E G + + SEL +G G Sbjct: 336 AGGDLIHGAREQWNDGANVLAIAPGEIIAYSRNHVTNKLFEENGIKVHRIPSSELSRGRG 395 Query: 281 SVKCCTL 287 +C ++ Sbjct: 396 GPRCMSM 402

MUL_1391

MUL_1396

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
MUL_1391	-1	19	1.287630	thymidine phosphorylase
MUL_1392	0	19	1.455362	cytidine deaminase
MUL_1393	0	18	1.708506	succinate dehydrogenase (cytochrome B-556
MUL_1394	0	13	2.317936	succinate dehydrogenase (hydrophobic membrane
MUL_1395	1	13	2.126963	succinate dehydrogenase flavoprotein subunit
MUL_1396	3	12	2.134342	succinate dehydrogenase iron-sulfur subunit

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_1391	DHBDHDRGNASE	59	5e-12	2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase signature. Length = 261 Score = 58.5 bits (141), Expect = 5e-12 Identities = 52/210 (24%), Positives = 88/210 (41%), Gaps = 22/210 (10%) Query: 21 GRVVVVTGANTGLGYHTAEALADRGAHVVLAVRNPEKGNAAVAQIVAAKPQADVTLQALD 80 G++ +TGA G+G A LA +GAH+ NPEK V+ + A A+ D Sbjct: 8 GKIAFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHAEAF--PAD 65 Query: 81 LSSLDSVRSAADALRSAYPRIDLLINNAGV--MWTPKQVTKDGFEMQFGTNHLGHFALTG 138 + ++ + ID+L+N AGV ++ + +E F N G F + Sbjct: 66 VRDSAAIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGVFNASR 125 Query: 139 LLLDHLLPVPGSRVITV-SSLGHRIRAAIHFDDLQWERSYNRVAAYGQSKLANLLFTYEL 197 + +++ ++TV S+ R ++ AAY SK A ++FT L Sbjct: 126 SVSKYMMDRRSGSIVTVGSNPAGVPRTSM--------------AAYASSKAAAVMFTKCL 171 Query: 198 QRRLAADSQAATIAVAAHPGDSNTELARNL 227 LA + I PG + T++ +L Sbjct: 172 GLELAEYNIRCNI---VSPGSTETDMQWSL 198
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_1393	PF06917	31	0.017	Periplasmic pectate lyase
>PF06917#Periplasmic pectate lyase Length = 555 Score = 30.7 bits (69), Expect = 0.017 Identities = 12/50 (24%), Positives = 25/50 (50%) Query: 193 FDKGYISGYFVTDAERQEAVLEDPYILLVSSKVSTVKDLLPLLEKVIQGG 242 F + Y G FV A+ + +++P L + + ++ +D L + + I G Sbjct: 477 FKRHYHRGLFVESAQHRYFRIDNPIALALLTLIAAKQDKLAAIPQFITNG 526
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_1395	cloacin	35	6e-04	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 35.1 bits (80), Expect = 6e-04 Identities = 24/96 (25%), Positives = 36/96 (37%), Gaps = 7/96 (7%) Query: 231 GIGSGNTGNSNVGLGNLGSGNVGFGNTGNGDFGFGLTGDHQFGFGGFNSGSGNVGIGNSG 290 G G G+ ++ GN+ G G G G G G + ++ GG SG G G Sbjct: 4 GDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHG 63 Query: 291 TGNVGFFNSGNGNMGIGNSGSLNSGLGNSGSMSTGF 326 N G G SG+ + + ++ GF Sbjct: 64 -------NGGGNGNSGGGSGTGGNLSAVAAPVAFGF 92 Score = 30.1 bits (67), Expect = 0.026 Identities = 20/78 (25%), Positives = 31/78 (39%), Gaps = 3/78 (3%) Query: 211 GSGNTGSSNVGTGNTGSSNIGIGSGNTGNSNVGLGNLGSGNVGFGNTGNGDFGFGLTGDH 270 G G+ ++ +GN G+G G + G + + G +G G G+ Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGN- 64 Query: 271 QFGFGGFNSGSGNVGIGN 288 G G NSG G+ GN Sbjct: 65 --GGGNGNSGGGSGTGGN 80
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_1396	UREASE	44	1e-06	Urea amidohydrolase (urease) protein signature.
>UREASE#Urea amidohydrolase (urease) protein signature. Length = 570 Score = 44.0 bits (104), Expect = 1e-06 Identities = 24/83 (28%), Positives = 36/83 (43%), Gaps = 11/83 (13%) Query: 9 YVGDVAVRDGIIVAVGP---PD--DSVN--GDAAGRVIDASGLLVTPGFVDLHTHYDGQS 61 D+ ++DG I A+G PD V VI G +VT G +D H H+ Sbjct: 84 VKADIGLKDGRIAAIGKAGNPDMQPGVTIIVGPGTEVIAGEGKIVTAGGMDSHIHF---- 139 Query: 62 IWSDRLTPSSAHGVTTVLMGNCG 84 I ++ + G+T +L G G Sbjct: 140 ICPQQIEEALMSGLTCMLGGGTG 162

MUL_1481

MUL_1492

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
MUL_1481	0	13	-0.238593	PE-PGRS family protein
MUL_1482	0	14	-0.550667	non-IS element not present in Mycobacterium
MUL_1483	-1	13	-0.347136	transposase for IS2404
MUL_1484	-1	12	-0.004918	EsaT-6 like protein EsxP
MUL_1485	-1	11	0.583624	EsaT-6 like protein EsxN
MUL_1486	0	12	0.728344	deoxyguanosinetriphosphate
MUL_1487	0	12	0.129642	DNA primase
MUL_1488	0	12	0.119473	hypothetical protein
MUL_1489	1	12	-0.113744	*hypothetical protein
MUL_1490	0	11	-1.461526	thioredoxin ThiX
MUL_1491	0	13	-1.809914	serine acetyltransferase CysE
MUL_1492	0	14	-2.024028	cysteine synthase a CysK1

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_1481	BCTERIALGSPC	29	0.041	Bacterial general secretion pathway protein C signa...
>BCTERIALGSPC#Bacterial general secretion pathway protein C signature. Length = 272 Score = 29.2 bits (65), Expect = 0.041 Identities = 15/53 (28%), Positives = 25/53 (47%), Gaps = 2/53 (3%) Query: 82 ARDRVLSARGLDVLLTDLEKQQALMAEVADDAARDRAIRRYGQLEERFVTLGG 134 D ++ GLD L D E+ + M +AD + R GQ ++ ++ GG Sbjct: 220 DNDMAVALNGLD--LRDAEQAKKAMERMADVHNFTLTVERDGQRQDIYMEFGG 270
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_1483	HTHTETR	68	2e-16	TetR bacterial regulatory protein HTH signature.
>HTHTETR#TetR bacterial regulatory protein HTH signature. Length = 215 Score = 68.1 bits (166), Expect = 2e-16 Identities = 32/177 (18%), Positives = 63/177 (35%), Gaps = 14/177 (7%) Query: 1 MPKVSEDHLAARRRQILDGARRCFAEYGYDKATVRRLEQAIGMSRGAIFHHFRDKDALFF 60 M + ++ R+ ILD A R F++ G ++ + +A G++RGAI+ HF+DK LF Sbjct: 1 MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFS 60 Query: 61 ALAHEDAERMADVAS--------------REGLIQVMRDMLAAPEQFDWLATRLEIARKL 106 + + ++ RE LI V+ + + + + Sbjct: 61 EIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVTEERRRLLMEIIFHKCEFV 120 Query: 107 RNDPEFSRGWAERSAELAAATTDRLRRQKQANRVRDDVPNEVLQCYLDLVLDGLVAR 163 + E L+ +A + D+ + + GL+ Sbjct: 121 GEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMTRRAAIIMRGYISGLMEN 177
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_1486	GPOSANCHOR	50	9e-09	Gram-positive coccus surface protein anchor signature.
>GPOSANCHOR#Gram-positive coccus surface protein anchor signature. Length = 539 Score = 50.4 bits (120), Expect = 9e-09 Identities = 36/202 (17%), Positives = 66/202 (32%), Gaps = 8/202 (3%) Query: 37 ANADSRTDSIAALIADVARANQRLDDLSAAVELEQEGVNKAMVAVETARDEAAAAEHELE 96 A + AAL A A + L+ + + A E LE Sbjct: 211 AKIKTLEAEKAALAARKADLEKALEGAMNFSTADSAKIKTLEAEKAALEARQAELEKALE 270 Query: 97 ASQQAVKDANAAIAAAQHRFD----TFAAATYMNGPSDSYLTATSPDEIIAAATAAKTLA 152 + +A I + A + + ++ + D + A+ A K L Sbjct: 271 GAMNFSTADSAKIKTLEAEKAALEAEKADLEHQSQVLNANRQSLRRD-LDASREAKKQLE 329 Query: 153 ASAQTVMANL---ERARTRQVNKESASRLAEQKADKAAEDAKTSQDAAVTALTDTQRKFD 209 A Q + E +R ASR A+++ + + + + + +R D Sbjct: 330 AEHQKLEEQNKISEASRQSLRRDLDASREAKKQLEAEHQKLEEQNKISEASRQSLRRDLD 389 Query: 210 QQREEVNRLAAERDEAEAKLQA 231 RE ++ +EA +KL A Sbjct: 390 ASREAKKQVEKALEEANSKLAA 411 Score = 45.4 bits (107), Expect = 3e-07 Identities = 32/194 (16%), Positives = 58/194 (29%), Gaps = 8/194 (4%) Query: 46 IAALIADVARANQRLDDLSAAVELEQEGVNKAMVAVETARDE-------AAAAEHELEAS 98 AAL A A + L+ + + A + A Sbjct: 185 KAALEARQAELEKALEGAMNFSTADSAKIKTLEAEKAALAARKADLEKALEGAMNFSTAD 244 Query: 99 QQAVKDANAAIAAAQHRFDTFAAATYMNGPSDSYLTATSPDEIIAAATAAKTLAASAQTV 158 +K A AA + R A + +A + A A + A + Sbjct: 245 SAKIKTLEAEKAALEARQAELEKALEGAMNFSTADSAKI-KTLEAEKAALEAEKADLEHQ 303 Query: 159 MANLERARTRQVNKESASRLAEQKADKAAEDAKTSQDAAVTALTDTQRKFDQQREEVNRL 218 L R ASR A+++ + + + + + +R D RE +L Sbjct: 304 SQVLNANRQSLRRDLDASREAKKQLEAEHQKLEEQNKISEASRQSLRRDLDASREAKKQL 363 Query: 219 AAERDEAEAKLQAA 232 AE + E + + + Sbjct: 364 EAEHQKLEEQNKIS 377 Score = 38.1 bits (88), Expect = 6e-05 Identities = 36/201 (17%), Positives = 63/201 (31%), Gaps = 8/201 (3%) Query: 35 AVANADSRTDSIAALIADVARANQRLDDLSAAVELEQEGVNKAMVAVETARDEAAAAEHE 94 A+ A + + + +A I + L+ A +E E AM + E E Sbjct: 163 ALEGAMNFSTADSAKIKTLEAEKAALEARQAELEKALE---GAMNFSTADSAKIKTLEAE 219 Query: 95 LEASQQAVKDANAAIAAAQHRFDTFAAATYMNGPSDSYLTATSPDEIIAAATAAKTLAAS 154 A D A+ A + +A + L A E+ A A + + Sbjct: 220 KAALAARKADLEKALEGAMNFSTADSAKIKTLEAEKAALEARQA-ELEKALEGAMNFSTA 278 Query: 155 AQTVMANLERARTRQVNKESASRLAEQKADKAAEDAKTSQD--AAVTALTDTQRKFDQQR 212 + LE + + + L Q A +D A+ A + + + Sbjct: 279 DSAKIKTLEAEKAAL--EAEKADLEHQSQVLNANRQSLRRDLDASREAKKQLEAEHQKLE 336 Query: 213 EEVNRLAAERDEAEAKLQAAR 233 E+ A R L A+R Sbjct: 337 EQNKISEASRQSLRRDLDASR 357
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_1488	HTHFIS	32	0.003	FIS bacterial regulatory protein HTH signature.
>HTHFIS#FIS bacterial regulatory protein HTH signature. Length = 484 Score = 32.5 bits (74), Expect = 0.003 Identities = 32/153 (20%), Positives = 58/153 (37%), Gaps = 23/153 (15%) Query: 48 IVGQD----QLVERMLVGLLAKGHVLLEGVPGVAKTL---AVETFARVVGGSFARIQ--- 97 +VG+ ++ + + +++ G G K L A+ + + G F I Sbjct: 139 LVGRSAAMQEIYRVLARLMQTDLTLMITGESGTGKELVARALHDYGKRRNGPFVAINMAA 198 Query: 98 FTPDLVPTDIVGTRIYRQGKEEFDTELGPVVANF-------LLADEINRAPAKVQSALLE 150 DL+ +++ G K F F L DEI P Q+ LL Sbjct: 199 IPRDLIESELFGHE-----KGAFTGAQTRSTGRFEQAEGGTLFLDEIGDMPMDAQTRLLR 253 Query: 151 VMAERHVS-IGGKTFPMPNPFLVMATQNPIEQE 182 V+ + + +GG+T + +V AT ++Q Sbjct: 254 VLQQGEYTTVGGRTPIRSDVRIVAATNKDLKQS 286
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_1491	DHBDHDRGNASE	112	6e-32	2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase signature. Length = 261 Score = 112 bits (280), Expect = 6e-32 Identities = 69/252 (27%), Positives = 122/252 (48%), Gaps = 21/252 (8%) Query: 24 RSVLVTGGNRGIGLAIAQRLATDGHRVAVTHRGSGAPEGLFGVE-----------CDVTD 72 + +TG +GIG A+A+ LA+ G +A E + DV D Sbjct: 9 KIAFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHAEAFPADVRD 68 Query: 73 NDAVDRAFKEVEEHQGPVEVLVSNAGLSADAFLIRMTEERFEKVIDANLTGAFRVAQRAS 132 + A+D +E GP+++LV+ AG+ + +++E +E N TG F ++ S Sbjct: 69 SAAIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGVFNASRSVS 128 Query: 133 RSMQRKKFGRLIFIGSVSGSWGIGNQANYAASKAGVIGMARSIARELSKVNVTANVVAPG 192 + M ++ G ++ +GS + A YA+SKA + + + EL++ N+ N+V+PG Sbjct: 129 KYMMDRRSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLELAEYNIRCNIVSPG 188 Query: 193 YIDTDMTRAL-------DERIQEGALQF---IPAKRVGTAAEVAGVVSFLASEDASYISG 242 +TDM +L ++ I+ F IP K++ +++A V FL S A +I+ Sbjct: 189 STETDMQWSLWADENGAEQVIKGSLETFKTGIPLKKLAKPSDIADAVLFLVSGQAGHITM 248 Query: 243 AVIPVDGGMGMG 254 + VDGG +G Sbjct: 249 HNLCVDGGATLG 260
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_1492	DHBDHDRGNASE	46	6e-08	2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase signature. Length = 261 Score = 45.8 bits (108), Expect = 6e-08 Identities = 50/275 (18%), Positives = 101/275 (36%), Gaps = 38/275 (13%) Query: 5 LEGKRILVSGIITDSSIAFHIARVAQEQGAQLVLTGFD----RMRLIQRIVDRLPQKAPL 60 +EGK ++G I +AR QGA + D ++ + + + A Sbjct: 6 IEGKIAFITG--AAQGIGEAVARTLASQGAHI--AAVDYNPEKLEKVVSSLKAEARHAEA 61 Query: 61 IELDVQNEEHLASLAGRVTEVIGEGNNLDGVVHSIGFMPQSGMGINPFFDAPYEDVSKGI 120 DV++ + + R+ +G +D +V+ G + E+ Sbjct: 62 FPADVRDSAAIDEITARIEREMG---PIDILVNVAGVLR-----PGLIHSLSDEEWEATF 113 Query: 121 HISAYSYASLAKALLPIM--NPGGSIVGMDFD----PTRAMPAYNWMTVAKSALESVNRF 174 +++ + ++++ M GSIV + + P +M AY +K+A + Sbjct: 114 SVNSTGVFNASRSVSKYMMDRRSGSIVTVGSNPAGVPRTSMAAYA---SSKAAAVMFTKC 170 Query: 175 VAREAGKYGVRSNLVAAGPIRTLAMSAIVGGALGEE-----AGAQIQLLEDGWDQRAPVG 229 + E +Y +R N+V+ G T ++ G E + + P+ Sbjct: 171 LGLELAEYNIRCNIVSPGSTETDMQWSLWADENGAEQVIKGSLETFKT-------GIPLK 223 Query: 230 WNMKDPTPVAKTVCAVLSEWLPATTGDIIFADGGA 264 + P+ +A V ++S T + DGGA Sbjct: 224 -KLAKPSDIADAVLFLVSGQAGHITMHNLCVDGGA 257

MUL_1500

MUL_1503

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
MUL_1500	-1	12	2.361215	formate dehydrogenase H FdhF
MUL_1501	-1	8	1.403987	lipoprotein LppP
MUL_1502	-2	8	1.377695	cation transporter p-type ATPase D CtpD
MUL_1503	-3	9	0.367006	thioredoxin TrxA

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_1500	HTHTETR	57	3e-12	TetR bacterial regulatory protein HTH signature.
>HTHTETR#TetR bacterial regulatory protein HTH signature. Length = 215 Score = 56.9 bits (137), Expect = 3e-12 Identities = 18/53 (33%), Positives = 30/53 (56%) Query: 8 RTARARIRDEALRLFAERGPDAVTMRDIATAAGVSPALLIRHYGSKDGLIEAV 60 + R I D ALRLF+++G + ++ +IA AAGV+ + H+ K L + Sbjct: 10 QETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSEI 62
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_1501	HTHFIS	85	3e-21	FIS bacterial regulatory protein HTH signature.
>HTHFIS#FIS bacterial regulatory protein HTH signature. Length = 484 Score = 85.3 bits (211), Expect = 3e-21 Identities = 38/129 (29%), Positives = 68/129 (52%), Gaps = 1/129 (0%) Query: 26 APRVLVVEDSETIREMVNEALADVGYHTDTRSDGEGLEEVLQGLRPDLVVLDVMLPGRDG 85 +LV +D IR ++N+AL+ GY S+ L + DLVV DV++P + Sbjct: 3 GATILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENA 62 Query: 86 FALIDVIREWG-DIGIVLITARDGLPDRLRGLDGGADDYAVKPFELAELVSRVGAVLRRR 144 F L+ I++ D+ +++++A++ ++ + GA DY KPF+L EL+ +G L Sbjct: 63 FDLLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAEP 122 Query: 145 GRLPRVVQV 153 R P ++ Sbjct: 123 KRRPSKLED 131
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_1502	PRTACTNFAMLY	35	5e-04	Pertactin virulence factor family signature.
>PRTACTNFAMLY#Pertactin virulence factor family signature. Length = 910 Score = 35.4 bits (81), Expect = 5e-04 Identities = 19/58 (32%), Positives = 22/58 (37%) Query: 104 SPDTTAGPAVPALPGPPPLIPPGGHQGPHGPPPPPPPPPGGPPPGPPPDATATAAVHT 161 + + P P P G Q P P P P P PP G A A AAV+T Sbjct: 559 NGNGQWSLVGAKAPPAPKPAPQPGPQPPQPPQPQPEAPAPQPPAGRELSAAANAAVNT 616
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_1503	V8PROTEASE	47	3e-08	V8 serine protease family signature.
>V8PROTEASE#V8 serine protease family signature. Length = 336 Score = 46.9 bits (111), Expect = 3e-08 Identities = 25/170 (14%), Positives = 58/170 (34%), Gaps = 31/170 (18%) Query: 72 DGSGGMGCTAGFLVRTNAGRTGILTAGHCNKE--GEASKVSINY------STGGGYVNIG 123 +G + G +V G+ +LT H G+ + + G Sbjct: 97 APTGTFIAS-GVVV----GKDTLLTNKHVVDATHGDPHALKAFPSAINQDNYPNGGFTAE 151 Query: 124 TFSQSVSEGLNGEAHDIGLITLDSGKIPQSPAIKAAVPVTGIAT--DLKVGQLLCKFGMK 181 ++ EG D+ ++ Q+ I V ++ + +V Q + G Sbjct: 152 QITKYSGEG------DLAIVKF--SPNEQNKHIGEVVKPATMSNNAETQVNQNITVTGYP 203 Query: 182 TGRAEC------GQVTDISASKVAFLAASECGDSGGPVYRLDDDGTAVAV 225 + G++T + + + ++ G+SG PV+ ++ + + Sbjct: 204 GDKPVATMWESKGKITYLKGEAMQYDLSTTGGNSGSPVF--NEKNEVIGI 251

MUL_1718

MUL_1726

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
MUL_1718	0	15	3.228682	site-specific tyrosine recombinase XerD
MUL_1719	-1	13	1.919017	O-methyltransferase
MUL_1720	-1	11	1.739118	myo-inositol-1-phosphate synthase
MUL_1721	-1	12	1.358363	transferase
MUL_1722	-1	10	-0.089747	sugar phosphate isomerases/epimerases
MUL_1723	-2	9	-0.163390	metal dependent hydrolase
MUL_1724	-2	12	-0.429250	hypothetical protein
MUL_1725	-2	12	-0.183578	hypothetical protein
MUL_1726	-1	13	-0.446199	transposase for IS2404

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_1718	cloacin	40	1e-05	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 40.5 bits (94), Expect = 1e-05 Identities = 41/120 (34%), Positives = 49/120 (40%), Gaps = 11/120 (9%) Query: 153 SGGTQSGGTGGSAGLIGNGGNGGNGFLGGAGGAAGSGGWLAGSGGNGGAGGSVTGVGEVG 212 SGG G G+ GN NGG LG GGA+ GW S N GGS +G+ G Sbjct: 2 SGGDGRGHNTGAHSTSGNI-NGGPTGLGVGGGASDGSGW--SSENNPWGGGSGSGIHWGG 58 Query: 213 GAGGAGGSAPLLGWGGNGGAGGDSTQGTGGRGGAGGAGGALAAIGGAGGAGGTGATAGGD 272 G+G G GGNG +GG S G A A+ GAGG + Sbjct: 59 GSGHGNG-------GGNGNSGGGSGTGGNLSAVAAPVAFGFPAL-STPGAGGLAVSISAG 110 Score = 36.2 bits (83), Expect = 3e-04 Identities = 36/106 (33%), Positives = 42/106 (39%), Gaps = 9/106 (8%) Query: 225 GWGGNGGAGGDSTQGTGGRGGAGGAGGALAAIGGAGGAGGTGATAGGDGGVGGEGSGRLF 284 G G N GA S GG G G GGA + G+G ++ + GG GSG + Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGGA---------SDGSGWSSENNPWGGGSGSGIHW 56 Query: 285 GLGGAGGAGGTGTTSGGVGGTGGAGGVAGVLVGAGVGGFGGMGGAG 330 G G G GG SGG GTGG V G G G Sbjct: 57 GGGSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG 102 Score = 34.3 bits (78), Expect = 0.001 Identities = 25/78 (32%), Positives = 34/78 (43%), Gaps = 4/78 (5%) Query: 394 GAGGNGGDGGNGGGLFNIGRGGDGGNGGNAGATGGNGGNIGVVANGTFTQTLFGDGGNGG 453 G G N G G + GG G G GA+ G+G + G + + GG G Sbjct: 6 GRGHNTGAHSTSGNI----NGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 454 NGGNGGTGGTPGTGGSGG 471 +G GG G + G G+GG Sbjct: 62 HGNGGGNGNSGGGSGTGG 79 Score = 33.5 bits (76), Expect = 0.002 Identities = 25/74 (33%), Positives = 30/74 (40%), Gaps = 7/74 (9%) Query: 360 GTATGGDGGAGGQGAALWGAGFGGDGAVGGNSFVGAGGNGGDGGNGGGLFNIGRGGDGGN 419 G GG G G G A G+G+ + G G+G GG G G GG GN Sbjct: 18 GNINGGPTGLGVGGGASDGSGWSSENNPWGG---GSGSGIHWGGGSGH----GNGGGNGN 70 Query: 420 GGNAGATGGNGGNI 433 G TGGN + Sbjct: 71 SGGGSGTGGNLSAV 84 Score = 31.6 bits (71), Expect = 0.008 Identities = 27/104 (25%), Positives = 37/104 (35%), Gaps = 3/104 (2%) Query: 180 GGAGGAAGSGGWLAGSGGNGGAGGSVTGVGEVGGAGGAGGSAPLLGWGGNGGAGGDSTQG 239 GG G +G NGG G G + G+G S+ WGG G+G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTG---LGVGGGASDGSGWSSENNPWGGGSGSGIHWGGG 59 Query: 240 TGGRGGAGGAGGALAAIGGAGGAGGTGATAGGDGGVGGEGSGRL 283 +G G G + G + A G + G+G L Sbjct: 60 SGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGL 103 Score = 31.6 bits (71), Expect = 0.008 Identities = 21/55 (38%), Positives = 23/55 (41%), Gaps = 1/55 (1%) Query: 354 GGVGGFGTATGGDGGAG-GQGAALWGAGFGGDGAVGGNSFVGAGGNGGDGGNGGG 407 GG G G G G+G WG G G GG S G GG G+ G G G Sbjct: 22 GGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNGNSGGGSG 76 Score = 30.8 bits (69), Expect = 0.015 Identities = 28/78 (35%), Positives = 32/78 (41%), Gaps = 1/78 (1%) Query: 324 GGMGGAGTTGGAGDVGGQGVTLTGLGVGGIGGVG-GFGTATGGDGGAGGQGAALWGAGFG 382 GG G TG G TGLGVGG G G+ + GG G G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 383 GDGAVGGNSFVGAGGNGG 400 G+G GNS G+G G Sbjct: 63 GNGGGNGNSGGGSGTGGN 80 Score = 30.1 bits (67), Expect = 0.022 Identities = 25/94 (26%), Positives = 32/94 (34%), Gaps = 5/94 (5%) Query: 382 GGDGAVGGNSFVGAGGNGGDGGNGGGLFNIGRGGDGGNGGNAGATGGNGGNIGVVANGTF 441 GGDG GN G G G+ G G + N GG+G I Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 442 TQTLFGDGGNGGNGGNGGTGGTPGTGGSGGILIG 475 G+GG GN G G G + + + G Sbjct: 63 -----GNGGGNGNSGGGSGTGGNLSAVAAPVAFG 91 Score = 29.7 bits (66), Expect = 0.027 Identities = 28/99 (28%), Positives = 30/99 (30%) Query: 123 GDGANGTATSPNGGAGGFLYGNGGNGYSFTSGGTQSGGTGGSAGLIGNGGNGGNGFLGGA 182 G G N A S +G G G G G + G S G GG G Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNG 65 Query: 183 GGAAGSGGWLAGSGGNGGAGGSVTGVGEVGGAGGAGGSA 221 GG SGG G V GAGG A Sbjct: 66 GGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 29.3 bits (65), Expect = 0.044 Identities = 20/69 (28%), Positives = 24/69 (34%) Query: 324 GGMGGAGTTGGAGDVGGQGVTLTGLGVGGIGGVGGFGTATGGDGGAGGQGAALWGAGFGG 383 GG G G GGA D G G G G+ G + G+GG G G G Sbjct: 22 GGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNGNSGGGSGTGGNL 81 Query: 384 DGAVGGNSF 392 +F Sbjct: 82 SAVAAPVAF 90
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_1720	IGASERPTASE	32	0.002	IgA-specific serine endopeptidase (S6) signature.
>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature. Length = 1541 Score = 32.3 bits (73), Expect = 0.002 Identities = 17/71 (23%), Positives = 23/71 (32%), Gaps = 8/71 (11%) Query: 144 AVLARLVARLARDVQPVPAPAYAAYAPEADQTAEEEPQDDPESKDPKSKDEATEEEAPKE 203 +V + D PVP PA A + + AE Q +SK E+ E Sbjct: 1009 SVPSNNEEIARVDEAPVPPPAPATPSETTETVAENSKQ--------ESKTVEKNEQDATE 1060 Query: 204 PEESEAEAEAE 214 E E Sbjct: 1061 TTAQNREVAKE 1071
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_1721	cloacin	42	3e-06	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 42.4 bits (99), Expect = 3e-06 Identities = 37/121 (30%), Positives = 46/121 (38%), Gaps = 9/121 (7%) Query: 336 GNGGAGGNGGAPGAANAIGGQGGQGGIGGNGGNGGNGGTASSSNTGPTPSAGTGGGGTGG 395 G G G N GA + I G G+GG G + G+ SS P G GG Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGG----GASDGSGWSSENNPWGGGSGSGIHWGG 58 Query: 396 GGGGGGGGGGGGASASGAVGGTGGGGGGGGAGAAAGTGAVGGGGGGAAPPAAPVAPPEME 455 G G G GGG G + GG+G GG A G GA A ++ + Sbjct: 59 GSGHGNGGGNGNSG-----GGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALS 113 Query: 456 A 456 A Sbjct: 114 A 114 Score = 42.0 bits (98), Expect = 4e-06 Identities = 33/88 (37%), Positives = 40/88 (45%), Gaps = 8/88 (9%) Query: 371 NGGTASSSNTGPTPSAGTGGGGTGGGGGGGGGGGGGGASA--------SGAVGGTGGGGG 422 +GG NTG ++G GG G G GGG G G S+ SG+ GGG G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 423 GGGAGAAAGTGAVGGGGGGAAPPAAPVA 450 G G +G G GG + AAPVA Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAAPVA 89 Score = 35.8 bits (82), Expect = 3e-04 Identities = 39/116 (33%), Positives = 49/116 (42%), Gaps = 4/116 (3%) Query: 302 TGGNGGLGGNGGAGGHGGINTGTRGTASASGGTGGNGGAGGN---GGAPGAANAIGGQGG 358 +GG+G G G IN G G G + G+G + N GG G+ GG G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 359 QGGIGGNGGNGGNGGTASSSNTGPTPSA-GTGGGGTGGGGGGGGGGGGGGASASGA 413 G GGNG +GG GT + + P A G T G GG G SA+ A Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAAIA 117 Score = 35.5 bits (81), Expect = 5e-04 Identities = 30/100 (30%), Positives = 36/100 (36%), Gaps = 1/100 (1%) Query: 132 GTGAAGGDGGWLVGNGGNGGSGAPGQAGGAGGSAGLWGAGGAGGAGGSATTPGGAGGAGG 191 G G G NGG G GGA +G W + GGS + GG+G Sbjct: 4 GDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSG-WSSENNPWGGGSGSGIHWGGGSGH 62 Query: 192 TGGANGLIGGGNGGVGGAGGAGAAGGAGAVGSTAQAGGTG 231 G GG G GG A AA A + + G G Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG 102 Score = 32.4 bits (73), Expect = 0.004 Identities = 29/107 (27%), Positives = 37/107 (34%), Gaps = 19/107 (17%) Query: 308 LGGNGGAGGHGGINTGTRGTASASGGTGGNGGAGGNGGAPGAANAIGGQGGQGGIGGNGG 367 + G G G + G ++ + G G GGA G N GG G G Sbjct: 1 MSGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGG-----GSGSGIH 55 Query: 368 NGGNGGTASSSNTGPTPSAGTGGGGTGGGGGGGGGGGGGGASASGAV 414 GG G G GG G GGG G GG ++ + V Sbjct: 56 WGGGS--------------GHGNGGGNGNSGGGSGTGGNLSAVAAPV 88 Score = 31.6 bits (71), Expect = 0.006 Identities = 32/113 (28%), Positives = 41/113 (36%) Query: 185 GAGGAGGTGGANGLIGGGNGGVGGAGGAGAAGGAGAVGSTAQAGGTGGDGGAGGANRQLV 244 G G G +G I GG G+G GGA G + + G G GG+ Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNG 65 Query: 245 SLFGAGGAGGADGAGGLGGNGGDATGFGMTGGDGAMGGAVTVPVNFLAHAGSD 297 G G G G A GF GA G AV++ L+ A +D Sbjct: 66 GGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAAIAD 118 Score = 30.1 bits (67), Expect = 0.023 Identities = 33/101 (32%), Positives = 38/101 (37%), Gaps = 1/101 (0%) Query: 202 GNGGVGGAGGAGAAGGAGAVGSTAQAGGTGGDGGAGGANRQLVSLFGAGGAGGADGAGGL 261 G G G GA + G G T G G G+G ++ G G+G G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSEN-NPWGGGSGSGIHWGGGSG 61 Query: 262 GGNGGDATGFGMTGGDGAMGGAVTVPVNFLAHAGSDGGIGT 302 GNGG G G G AV PV F A S G G Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG 102 Score = 29.3 bits (65), Expect = 0.037 Identities = 28/75 (37%), Positives = 33/75 (44%), Gaps = 13/75 (17%) Query: 257 GAGGLGGNGGDATGFGMTGGDGAMGGAVTVPVNFLAHAGSDGGIGTGGNGGLGGNGGAGG 316 G GLG GG + G G + + GG GS GI GG G GNGG G Sbjct: 23 GPTGLGVGGGASDGSGWSSENNPWGG------------GSGSGIHWGGGSG-HGNGGGNG 69 Query: 317 HGGINTGTRGTASAS 331 + G +GT G SA Sbjct: 70 NSGGGSGTGGNLSAV 84
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_1722	NUCEPIMERASE	36	7e-04	Nucleotide sugar epimerase signature.
>NUCEPIMERASE#Nucleotide sugar epimerase signature. Length = 334 Score = 35.9 bits (83), Expect = 7e-04 Identities = 51/252 (20%), Positives = 77/252 (30%), Gaps = 77/252 (30%) Query: 780 TVLLTGATGFLGRYLALEWLERMDLV-------DGKLICLVRA-------------KSDT 819 L+TGA GF+G +++ LE V D + L +A K D Sbjct: 2 KYLVTGAAGFIGFHVSKRLLEAGHQVVGIDNLNDYYDVSLKQARLELLAQPGFQFHKIDL 61 Query: 820 EARARLEKTFDSGAPELLAHYRALAGDHLEVLAGDKGEADLGLDRQTWQRLADTVDLIVD 879 R + F SG E + R + + + Sbjct: 62 ADREGMTDLFASGHFERVFISPHRLA-----------------VRYSLE----------N 94 Query: 880 PAALVNHVLPYSQLFGPNALGTAELLRLALTSKIKPYSYTSTIGVADQIPPSAFTEDADI 939 P A + N G +L +KI+ Y S+ V F+ D + Sbjct: 95 PHAYAD----------SNLTGFLNILEGCRHNKIQHLLYASSSSVYGLNRKMPFSTDDSV 144 Query: 940 RVISATRAVDDSYANGYSNSKWAGEVLLREAHVLCGLPVAVFRCDMILADTTWAGQLNVP 999 D + Y+ +K A E++ L GLP R T G P Sbjct: 145 ----------DHPVSLYAATKKANELMAHTYSHLYGLPATGLRF------FTVYGPWGRP 188 Query: 1000 DM----FTRMIL 1007 DM FT+ +L Sbjct: 189 DMALFKFTKAML 200
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_1725	SECFTRNLCASE	57	9e-11	Bacterial translocase SecF protein signature.
>SECFTRNLCASE#Bacterial translocase SecF protein signature. Length = 333 Score = 56.8 bits (137), Expect = 9e-11 Identities = 32/197 (16%), Positives = 74/197 (37%), Gaps = 24/197 (12%) Query: 386 QLANVLKYGSLPLSFESSEAQTVSATLGLTSLRAGLIAGAIGLALVLLY-SLLYYRVLGL 444 ++ L L S E +V + + + + +++ Y + + L Sbjct: 123 KVETALTAVDPALKITSFE--SVGPKVSGELVWTAVWSLLAATVVIMFYIWVRFEWQFAL 180 Query: 445 LTALSLIASGAMVFAILVLLGRYINYTLDLAGIAGLIIGIGTTADSFVVFFERIKDEIRE 504 ++L+ + + +L + L +A L+ G + + VV F+R+++ + + Sbjct: 181 GAVVALVHDVLLTVGLFAVLQLKFD----LTTVAALLTITGYSINDTVVVFDRLRENLIK 236 Query: 505 GRSFR------SAVPRGWARARKTIVSGNAVTFLAAAVLYFLAIGQVKGFAFTLGLTTIL 558 ++ +V +R T ++ T LA + ++GF F + Sbjct: 237 YKTMPLRDVMNLSVNETLSRTVMTGMT----TLLALVPMLIWGGDVIRGFVFAM------ 286 Query: 559 DIVVVFLVTWPLVYLAS 575 + VF T+ VY+A Sbjct: 287 -VWGVFTGTYSSVYVAK 302 Score = 36.7 bits (85), Expect = 2e-04 Identities = 22/126 (17%), Positives = 44/126 (34%), Gaps = 12/126 (9%) Query: 14 LSVFLVLLIGVYLLVF-LTGDKKAAPKLGIDLQGGTRVTLTARTPDGSAPSREALAQAQQ 72 ++ +++ + LV L GID +GGT + + T R AL + + Sbjct: 26 AAIVMMIASVILPLVIGLN--------FGIDFKGGTTIRTESTTAIDVGVYRAAL-EPLE 76 Query: 73 IISARVNGLGVSGSEVVVDGDNLVITVPGNDGNEARNLGQTARLYIRPVMNSM-PAQPAA 131 + ++ + S ++ DG A G + + V ++ PA Sbjct: 77 LGDVIISEVR-DPSFREDQHVAMIRIQMQEDGQGAEGQGAQGQELVNKVETALTAVDPAL 135 Query: 132 QEPQQE 137 + E Sbjct: 136 KITSFE 141
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_1726	SECFTRNLCASE	258	5e-85	Bacterial translocase SecF protein signature.
>SECFTRNLCASE#Bacterial translocase SecF protein signature. Length = 333 Score = 258 bits (660), Expect = 5e-85 Identities = 75/310 (24%), Positives = 146/310 (47%), Gaps = 20/310 (6%) Query: 60 FEVVGRRKLWYGISGAIMAIAILSIIVRGFTFGIDFKGGTTVSFP----------RGDSQ 109 F+ + +G + +M +++ +V G FGIDFKGGTT+ R + Sbjct: 14 FDFFRWQWATFGAAIVMMIASVILPLVIGLNFGIDFKGGTTIRTESTTAIDVGVYRAALE 73 Query: 110 VTQVEEVFHNVVGSDPESVVTVGSGASATVQIRSETLSNEQTEKLRDALFDAFHPKGADG 169 ++ +V + V S A +Q++ + E L + Sbjct: 74 PLELGDVIISEVRD--PSFREDQHVAMIRIQMQEDGQGAEGQGAQGQELVNKVETALTAV 131 Query: 170 KPSKKAISDAAVSETWGGQITKKAVIALVVFLVLVAIYITVRYERYMTISAIAAMIFDLT 229 P+ K S +V G++ AV +L+ V++ YI VR+E + A+ A++ D+ Sbjct: 132 DPALKITSFESVGPKVSGELVWTAVWSLLAATVVIMFYIWVRFEWQFALGAVVALVHDVL 191 Query: 230 VTAGVYSLVGFEVTPATVIGLLTILGFSLYDTVIVFDKVEENTHGFQHTTRRTFAEQANL 289 +T G+++++ + TV LLTI G+S+ DTV+VFD++ EN ++ R + NL Sbjct: 192 LTVGLFAVLQLKFDLTTVAALLTITGYSINDTVVVFDRLRENLIKYKTMPLR---DVMNL 248 Query: 290 AVNQTFMRSINTSLISVLPVLSLMVVAVWLLGVGTLKDLALVQLIGIIVGTYSSIFFATP 349 +VN+T R++ T + ++L ++ +++ G ++ + G+ GTYSS++ A Sbjct: 249 SVNETLSRTVMTGMTTLLALVPMLI-----WGGDVIRGFVFAMVWGVFTGTYSSVYVAKN 303 Query: 350 LLVTLRERTE 359 +++ + Sbjct: 304 IVLFIGLDRN 313

MUL_2031

MUL_2045

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
MUL_2031	1	10	2.321320	hypothetical protein
MUL_2032	2	10	2.311651	hypothetical protein
MUL_2033	3	11	2.083546	hypothetical protein
MUL_2034	1	13	2.130818	acyl-CoA synthetase
MUL_2036	1	12	2.245225	chorismate pyruvate-lyase
MUL_2038	0	11	2.097356	acyl-CoA synthetase
MUL_2039	0	10	1.903210	polyketide synthase
MUL_2040	-1	10	1.846359	lipoprotein LppX
MUL_2041	-2	12	1.746857	transmembrane transport protein MmpL7
MUL_2042	-2	11	1.312572	acyl-CoA synthetase
MUL_2043	1	14	0.455434	methyltransferase
MUL_2044	-1	14	0.581495	multifunctional mycocerosic acid synthase
MUL_2045	-1	14	0.560383	acyltransferase PapA5

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_2031	DNABINDINGHU	28	0.011	Prokaryotic integration host factor signature.
>DNABINDINGHU#Prokaryotic integration host factor signature. Length = 91 Score = 28.1 bits (63), Expect = 0.011 Identities = 10/29 (34%), Positives = 14/29 (48%) Query: 205 AATLTRRQLGAVLDAAADVMREALAKGGT 233 A LT++ A +DA + LAKG Sbjct: 14 ATELTKKDSAAAVDAVFSAVSSYLAKGEK 42
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_2033	CHANLCOLICIN	35	0.001	Channel forming colicin signature.
>CHANLCOLICIN#Channel forming colicin signature. Length = 522 Score = 35.4 bits (81), Expect = 0.001 Identities = 61/314 (19%), Positives = 111/314 (35%), Gaps = 44/314 (14%) Query: 249 ATMRREHDEAAARLTVAAEELAAHEATLAELTSRAESVQHTWFGLSALAERVGTTVRIAN 308 A +++ E AAR AAE A +A LT R + + + L A R + +A+ Sbjct: 60 AQLKKTQAEQAARAKAAAEAQAKAKANRDALTQRLKDIVNE--ALRHNASRTPSATELAH 117 Query: 309 ERAQHLDVEPVTNSDTDPDALDAEAEQVAIAEQQLLVELAEARDRLDAARAELADREHRA 368 A+ AE E++ +A+ + +AR +AA + E R Sbjct: 118 ANNA---------------AMQAEDERLRLAKAE-----EKARKEAEAAEKAFQEAEQRR 157 Query: 369 AEADRAH------LAAVRAEADRREGLARLAGQVETMRARVESIDDSVARLSERIEHAAA 422 E +R L AE R L+ A VE + ++ + V ++ I+ + Sbjct: 158 KEIEREKAETERQLKLAEAEEKRLAALSEEAKAVEIAQKKLSAAQSEVVKMDGEIKTLNS 217 Query: 423 RA----QQTRAEFETVQARVGELDQGEVGLDEQHERTVAALRLAE------------QRL 466 R AE +T+ + EL Q E E A +R Sbjct: 218 RLSSSIHARDAEMKTLAGKRNELAQASAKYKELDELVKKLSPRANDPLQNRPFFEATRRR 277 Query: 467 AELQVAERDAERQVASLRARIGALSVGLDRKDGAAWLARNHSDAGLFGSVAQLVKVRSGY 526 + ++QV + RI ++ + + A N+ +AG+ ++ Sbjct: 278 VGAGKIREEKQKQVTASETRINRINADITQIQKAISQVSNNRNAGIARVHEAEENLKKAQ 337 Query: 527 EAAVAAVLGSAAEA 540 + + + A +A Sbjct: 338 NNLLNSQIKDAVDA 351
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_2034	IGASERPTASE	38	7e-05	IgA-specific serine endopeptidase (S6) signature.
>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature. Length = 1541 Score = 38.1 bits (88), Expect = 7e-05 Identities = 37/212 (17%), Positives = 66/212 (31%), Gaps = 17/212 (8%) Query: 29 RRRRISLAARPEVES------KDRSGGYTASSGITFSQTPTTTEPADRIDTSGLPAVGDD 82 + R ++ A+ V++ +SG T + T ++ T E ++ Sbjct: 1064 QNREVAKEAKSNVKANTQTNEVAQSGSETKETQTTETKETATVEKEEKAKVETEKTQEVP 1123 Query: 83 ATIPRDAPKRTISEVHLPEIEPEPET-PAPVAAEPVAP--------PVAPETPQAPEVPE 133 + +PK+ SE P+ EP E P EP + A ET E P Sbjct: 1124 KVTSQVSPKQEQSETVQPQAEPARENDPTVNIKEPQSQTNTTADTEQPAKETSSNVEQPV 1183 Query: 134 APEAPE--APEVAEAEVIEPPEGRLERLRGRLAKSQNALGRSVLGLIGGGDLDEDSWQDV 191 V E P + + R + + + + Sbjct: 1184 TESTTVNTGNSVVENPENTTPATTQPTVNSESSNKPKNRHRRSVRSVPHNVEPATTSSND 1243 Query: 192 EDTLLVADLGPVVTEAVVSQLRSRLASGNVRS 223 T+ + DL T AV+S R++ + Sbjct: 1244 RSTVALCDLTSTNTNAVLSDARAKAQFVALNV 1275
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_2039	AUTOINDCRSYN	27	0.006	Autoinducer synthesis protein signature.
>AUTOINDCRSYN#Autoinducer synthesis protein signature. Length = 216 Score = 26.7 bits (59), Expect = 0.006 Identities = 6/21 (28%), Positives = 12/21 (57%) Query: 36 SSRLGWRLEVNDGGQWAFFDD 56 RL W ++ DG ++ +D+ Sbjct: 29 KDRLNWAVQCTDGMEFDQYDN 49
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_2042	YERSSTKINASE	32	0.007	Yersinia serine/threonine protein kinase signature.
>YERSSTKINASE#Yersinia serine/threonine protein kinase signature. Length = 732 Score = 32.4 bits (73), Expect = 0.007 Identities = 22/75 (29%), Positives = 36/75 (48%), Gaps = 2/75 (2%) Query: 133 GLVHGDVKPADIVMTNAGEGQPRILLKGFGIAAPHGAPGDATGFVAPEQLTG-AEADGRS 191 G+VH D+KP ++V A G+P ++ G + G F APE G A +S Sbjct: 265 GVVHNDIKPGNVVFDRA-SGEPVVIDLGLHSRSGEQPKGFTESFKAPELGVGNLGASEKS 323 Query: 192 DQYALAATAMILLTG 206 D + + +T + + G Sbjct: 324 DVFLVVSTLLHCIEG 338
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_2043	UREASE	32	0.011	Urea amidohydrolase (urease) protein signature.
>UREASE#Urea amidohydrolase (urease) protein signature. Length = 570 Score = 31.6 bits (72), Expect = 0.011 Identities = 27/97 (27%), Positives = 43/97 (44%), Gaps = 15/97 (15%) Query: 4 DVIIRDGLWFDGTGSAPQTRTLGIRDGMVATV-SAGPLDET-------GCA-QVIDAAGK 54 D +I + L D G +G++DG +A + AG D G +VI GK Sbjct: 69 DTVITNALILDHWGIVKAD--IGLKDGRIAAIGKAGNPDMQPGVTIIVGPGTEVIAGEGK 126 Query: 55 WVMPGFIDVHTHYDAEVLLDPGLRESVRHGVTTVLLG 91 V G +D H H+ ++ E++ G+T +L G Sbjct: 127 IVTAGGMDSHIHFICPQQIE----EALMSGLTCMLGG 159
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_2044	HTHTETR	46	2e-08	TetR bacterial regulatory protein HTH signature.
>HTHTETR#TetR bacterial regulatory protein HTH signature. Length = 215 Score = 45.8 bits (108), Expect = 2e-08 Identities = 28/166 (16%), Positives = 62/166 (37%), Gaps = 6/166 (3%) Query: 1 MARTQQQRREETVARLLQASIDTIVGVGYARASAAIITKRAGVSVGALFRHFETMGDFMA 60 MAR +Q +ET +L ++ G + S I K AGV+ GA++ HF+ D + Sbjct: 1 MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFS 60 Query: 61 ATAYEVLRRQLDIFTKQVAEIPADRPAL--EAALAILRDITAGATN----AVLYELMIAA 114 ++ + A+ P D ++ E + +L +++ Sbjct: 61 EIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVTEERRRLLMEIIFHKCEFV 120 Query: 115 RTDDKLKETLQHVLGQYRAKIYDAARTLPGAEGFPEGTFPAIVAVL 160 +++ +++ + +I + A+ P A++ Sbjct: 121 GEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMTRRAAII 166
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_2045	BLACTAMASEA	36	1e-04	Beta-lactamase class A signature.
>BLACTAMASEA#Beta-lactamase class A signature. Length = 286 Score = 35.9 bits (83), Expect = 1e-04 Identities = 26/99 (26%), Positives = 38/99 (38%), Gaps = 7/99 (7%) Query: 48 DLDTGQVLAGRDQNVTHPPASTIKVLLALVALDE-----LNLNSTVVADEADTHVECNCV 102 DL +G+ L + P ST KV+L L L + + D V+ + V Sbjct: 46 DLASGRTLTAWRADERFPMMSTFKVVLCGAVLARVDAGDEQLERKIHYRQQDL-VDYSPV 104 Query: 103 GVK-AGHTYTARQLLDGLLLVSGNDAANTLAQLLGGQDA 140 K T +L + +S N AAN L +GG Sbjct: 105 SEKHLADGMTVGELCAAAITMSDNSAANLLLATVGGPAG 143

MUL_2477

MUL_2485

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
MUL_2477	-2	11	0.014577	oxidoreductase
MUL_2480	3	10	4.010279	NADPH quinone oxidoreductase FadB4
MUL_2481	4	11	4.016828	PPE family protein
MUL_2482	3	12	3.636872	transposase for IS2606
MUL_2483	3	11	3.543678	transposase for IS2404
MUL_2484	3	12	4.242224	oxidoreductase
MUL_2485	4	13	4.545007	hypothetical protein

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_2477	HTHFIS	40	7e-06	FIS bacterial regulatory protein HTH signature.
>HTHFIS#FIS bacterial regulatory protein HTH signature. Length = 484 Score = 40.2 bits (94), Expect = 7e-06 Identities = 29/138 (21%), Positives = 51/138 (36%), Gaps = 16/138 (11%) Query: 27 RAALTLILTAVLARGHVLIEDLPGLGKTLIARS---FAAALGLEFKRVQ---FTPDLLPA 80 + ++ + ++I G GK L+AR+ + F + DL+ + Sbjct: 147 QEIYRVLARLMQTDLTLMITGESGTGKELVARALHDYGKRRNGPFVAINMAAIPRDLIES 206 Query: 81 DLLG------STIYDMQSGRFEFRAGPIFTNLLMADEINRTPPKTQAALLEAMAEGQVSI 134 +L G + +GRFE G L DEI P Q LL + +G+ + Sbjct: 207 ELFGHEKGAFTGAQTRSTGRFEQAEG----GTLFLDEIGDMPMDAQTRLLRVLQQGEYTT 262 Query: 135 DGQTHKLPVPFIVLATDN 152 G + ++A N Sbjct: 263 VGGRTPIRSDVRIVAATN 280
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_2481	DHBDHDRGNASE	44	2e-07	2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase signature. Length = 261 Score = 44.3 bits (104), Expect = 2e-07 Identities = 38/173 (21%), Positives = 61/173 (35%), Gaps = 34/173 (19%) Query: 21 VAQIEAAGGR-AVAVRADLTDRDDVAALVTAARDSLGPITILVNNAAFTAPGRPPVPGAA 79 V A R A A AD+ D + + +GPI ILVN A PG Sbjct: 48 VVSSLKAEARHAEAFPADVRDSAAIDEITARIEREMGPIDILVNVAGVLRPG-------- 99 Query: 80 PRAKSSRAAVGKPGWPGFVSVPLAAYRRHFETSVFAAYELMQLSCPDMIAAGAGSIINIT 139 S+ + F + + + M+ +GSI+ + Sbjct: 100 ----------------LIHSLSDEEWEATFSVNSTGVFNASRSVSKYMMDRRSGSIVTVG 143 Query: 140 SVASRLPGDGPYPDRSGGVLPGYGGSKAALEHLTQCAAFDLADHHIAVNALAP 192 S + +P + Y SKAA T+C +LA+++I N ++P Sbjct: 144 SNPAGVPRTS---------MAAYASSKAAAVMFTKCLGLELAEYNIRCNIVSP 187
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_2482	HTHTETR	77	1e-19	TetR bacterial regulatory protein HTH signature.
>HTHTETR#TetR bacterial regulatory protein HTH signature. Length = 215 Score = 77.0 bits (189), Expect = 1e-19 Identities = 27/169 (15%), Positives = 55/169 (32%), Gaps = 11/169 (6%) Query: 12 PGAGRPRDPRIDFAILSATMELLVQIGYSNLSLAAVAERAGTTKSALYRRWSSKAELVHE 71 + IL + L Q G S+ SL +A+ AG T+ A+Y + K++L E Sbjct: 2 ARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSE 61 Query: 72 AAFPVTPSALSAPAGDFAGDIRMMVAATRDV----FTTPVVRAALPGLV------ADMTA 121 + A ++ R++ + V L+ + Sbjct: 62 IWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVTEERRRLLMEIIFHKCEFVG 121 Query: 122 EADLNARVLSRF-TELFATVRIRLQEAVDRGEAHPDVDPNRMIELIGGA 169 E + + E + + L+ ++ D+ R ++ G Sbjct: 122 EMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMTRRAAIIMRGY 170
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_2485	cloacin	37	2e-04	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 37.4 bits (86), Expect = 2e-04 Identities = 32/109 (29%), Positives = 40/109 (36%), Gaps = 4/109 (3%) Query: 232 AGGYGPGGVGGTGGAGGAGGLLAGLVGAGGGHGGTGGFGAGGTGGDGGAGGNAGLFGGPG 291 +GG G G G G +G GGG G+ + GG+G GG G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 292 GAGGTGGVGTGGDGGNGGAGGNAGALFGTG----GAGGAGGSGVAGAGG 336 G G +GG G GG A G GAGG V+ + G Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAG 110 Score = 37.0 bits (85), Expect = 3e-04 Identities = 38/139 (27%), Positives = 46/139 (33%), Gaps = 12/139 (8%) Query: 266 TGGFGAGGTGGDGGAGGNAGLFGGPGGAGGTGGVGTG----------GDGGNGGAGGNAG 315 +GG G G G GN GGP G G GG G G G G G Sbjct: 2 SGGDGRGHNTGAHSTSGNIN--GGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGG 59 Query: 316 ALFGTGGAGGAGGSGVAGAGGVGGAGGNAGLLFSAGGVGGAGGYGSSDGGAGGAGGNGGL 375 + G GG G G G G + F A GAGG S + + Sbjct: 60 SGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAAIADI 119 Query: 376 LYSNGGVGGTGGYGAAAAG 394 + + G G +G A G Sbjct: 120 MAALKGPFKFGLWGVALYG 138 Score = 35.5 bits (81), Expect = 9e-04 Identities = 30/128 (23%), Positives = 43/128 (33%), Gaps = 11/128 (8%) Query: 365 GAGGAGGNGGLLYSNGGVGGTGGYGAAAAGGVGGAGGRAGLAIGGGGAGGAGGEGATTGG 424 G G G N G ++G + G G G+G + GGG+G G +G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 425 DGGAGGTGVLIGNGGNAGVGGTGPAAGATGVGGTSGLLLGLDGFNAPASTSPLHTFQQQA 484 GNGG G G G G + + G + P + + A Sbjct: 63 -----------GNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGA 111 Query: 485 LGAVNAPV 492 L A A + Sbjct: 112 LSAAIADI 119 Score = 33.9 bits (77), Expect = 0.002 Identities = 37/115 (32%), Positives = 44/115 (38%), Gaps = 2/115 (1%) Query: 571 GAGGAGGYSSTADGGVGGAGGAGGLWGGGGIGGTGGFGALNGAAGGVGGAGGLLGGLVGA 630 G G G + GG GL GGG G+ + N GG G+G GG G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 631 GGGDGGAGGYGLTGAGGAGGAGGNSGLLSGPGGSGGTGGAGAVADGAVGGAGGSA 685 G G G G +G GG A P S T GAG +A GA +A Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALS--TPGAGGLAVSISAGALSAA 115 Score = 33.9 bits (77), Expect = 0.003 Identities = 40/134 (29%), Positives = 52/134 (38%), Gaps = 4/134 (2%) Query: 140 GDGGIGGSGTPGTVANPTGGVGGVGGAAGLLGSGGAGGAGGSSAFGDGGAGGVGGWLSGN 199 GDG +G T N GG G+G G S G+G + ++ +G G G+ G Sbjct: 4 GDGRGHNTGAHSTSGNINGGPTGLGVGGG--ASDGSGWSSENNPWGGGSGSGIH--WGGG 59 Query: 200 AGAGGAGGPGLFGFNGGAGGAGGLLGAGGLGGAGGYGPGGVGGTGGAGGAGGLLAGLVGA 259 +G G GG G G G GG + A G G GG + AG L A + Sbjct: 60 SGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAAIADI 119 Query: 260 GGGHGGTGGFGAGG 273 G FG G Sbjct: 120 MAALKGPFKFGLWG 133 Score = 33.5 bits (76), Expect = 0.003 Identities = 35/119 (29%), Positives = 42/119 (35%), Gaps = 12/119 (10%) Query: 336 GVGGAGGNAGLLFSAGGVGGAGGYGSSDGGAGGAGGNGGLLYSNGGVGGTGGYGAAAAGG 395 G G G N G ++G + G G GGA G N GG G G GG Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGL---GVGGGASDGSGWSSENNPWGGGSGSGIHWGGG 59 Query: 396 VGGAGGRAGLAIGGGGAGGAGGEGATTGGDGGAGGTGVLIGNGGNAGVGGTGPAAGATG 454 G G GG G G G+ TGG+ A V G + G G A + Sbjct: 60 SGH---------GNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISA 109 Score = 33.1 bits (75), Expect = 0.004 Identities = 36/118 (30%), Positives = 45/118 (38%), Gaps = 5/118 (4%) Query: 503 NGTPGAAGSGAAGTAGGWVFGDGGADGSGAMSTGADGGAGGAAGMWGTGGSGGAGPAGIG 562 N + G G G G +DGSG S G G +G+ GGSG Sbjct: 10 NTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHG-----N 64 Query: 563 GGLTGGAGGAGGAGGYSSTADGGVGGAGGAGGLWGGGGIGGTGGFGALNGAAGGVGGA 620 GG G +GG G GG S V A G GG+ + GAL+ A + A Sbjct: 65 GGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAAIADIMAA 122 Score = 32.8 bits (74), Expect = 0.006 Identities = 29/80 (36%), Positives = 35/80 (43%), Gaps = 5/80 (6%) Query: 540 GAGGAAGMWGTGGSGGAGPAGIGGGLTGGAGGAGGAGGYSSTADGGVGGAGGAGGLWGGG 599 G G G T G+ GP G+G G GGA G+SS + GG+G GG Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVG-----GGASDGSGWSSENNPWGGGSGSGIHWGGGS 60 Query: 600 GIGGTGGFGALNGAAGGVGG 619 G G GG G G +G G Sbjct: 61 GHGNGGGNGNSGGGSGTGGN 80 Score = 32.4 bits (73), Expect = 0.008 Identities = 26/78 (33%), Positives = 37/78 (47%), Gaps = 2/78 (2%) Query: 637 AGGYGLTGAGGAGGAGGN--SGLLSGPGGSGGTGGAGAVADGAVGGAGGSAGLLFGSGRI 694 +GG G GA GN G G G + G+G ++ G G +G+ +G G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 695 GGDGGFGSNTGGAGGSGG 712 G+GG N+GG G+GG Sbjct: 62 HGNGGGNGNSGGGSGTGG 79 Score = 31.6 bits (71), Expect = 0.012 Identities = 33/105 (31%), Positives = 38/105 (36%), Gaps = 6/105 (5%) Query: 171 GSGGAGGAGGSSAFGDGGAGGVGGWLSGNAGAGGAGGPGLFGFNGGAGGAGGLLGAGGLG 230 G G GA +S +GG G+G G G + G G N GG G G G Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGV------GGGASDGSGWSSENNPWGGGSGSGIHWGGG 59 Query: 231 GAGGYGPGGVGGTGGAGGAGGLLAGLVGAGGGHGGTGGFGAGGTG 275 G G G GG+G G L A G GAGG Sbjct: 60 SGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 30.8 bits (69), Expect = 0.020 Identities = 28/94 (29%), Positives = 37/94 (39%), Gaps = 5/94 (5%) Query: 597 GGGGIGGTGGFGALNGAAGGVGGAGGLLGGLVGAGGGDGGAGGYGLTGAGGAGGAGGNSG 656 GG G G G + +G G G VG G DG GG G+G + G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLG-----VGGGASDGSGWSSENNPWGGGSGSGIHWG 57 Query: 657 LLSGPGGSGGTGGAGAVADGAVGGAGGSAGLLFG 690 SG G GG G +G + + +A + FG Sbjct: 58 GGSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFG 91 Score = 30.8 bits (69), Expect = 0.021 Identities = 41/113 (36%), Positives = 46/113 (40%), Gaps = 7/113 (6%) Query: 120 NGANGAPGTGANGGDGGWLLGDGGIGGSGTPGTVANPTGGVGGVGGAAGLLGSGGAGGAG 179 N + NGG G +G G GSG + NP GG G G G G G G G Sbjct: 10 NTGAHSTSGNINGGPTGLGVGGGASDGSGW-SSENNPWGGGSGSGIHWG--GGSGHGNGG 66 Query: 180 GSSAFGDGGAGGVGGWLSGNAGAGGAGGPGLFGFNGGAGGAGGLLGAGGLGGA 232 G+ GG G GG LS A G P L GAGG + AG L A Sbjct: 67 GNG--NSGGGSGTGGNLSAVAAPVAFGFPAL--STPGAGGLAVSISAGALSAA 115 Score = 30.5 bits (68), Expect = 0.026 Identities = 29/107 (27%), Positives = 39/107 (36%), Gaps = 7/107 (6%) Query: 614 AGGVGGAGGLLGGLVGAGGGDGGAGGYGLTGAGGAGGAGGNSGLLSGPGGSGGTGGAGAV 673 G +G + GG G G G G + G G + G G SG+ G G G GG Sbjct: 11 TGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGN-- 68 Query: 674 ADGAVGGAGGSAGLLFGSGRIGGDGGFGSNTGGAGGSGGLLLGQDGS 720 G +GG +G + FG G+GGL + Sbjct: 69 -----GNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAG 110 Score = 30.1 bits (67), Expect = 0.034 Identities = 33/104 (31%), Positives = 36/104 (34%), Gaps = 4/104 (3%) Query: 159 GVGGVGGAAGLLGSGGAGGAGGSSAFGDGGAGGVGGWLSGNAGAGGAGGPGLFGFNGGAG 218 G G G G + G G + GGA GW S N GG G G+ G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 219 GAGGLLGAGGLGGAGGYGPGGVGGTGGAGGAGGLLAGLVGAGGG 262 G GG G GG G GG A A G A GG Sbjct: 63 GNGG----GNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG 102 Score = 30.1 bits (67), Expect = 0.035 Identities = 36/121 (29%), Positives = 43/121 (35%), Gaps = 14/121 (11%) Query: 300 GTGGDGGNGGAGGNAGALFGTGGAGGAGGSGVAGAGGVGGAGGNAGLLFSAGGV--GGAG 357 G G G N GA +G + G G GG G + G +S+ GG Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGG------------GASDGSGWSSENNPWGGGS 50 Query: 358 GYGSSDGGAGGAGGNGGLLYSNGGVGGTGGYGAAAAGGVGGAGGRAGLAIGGGGAGGAGG 417 G G GG G G GG S GG G G A AA G + GG + G Sbjct: 51 GSGIHWGGGSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAG 110 Query: 418 E 418 Sbjct: 111 A 111

MUL_2542

MUL_2550

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
MUL_2542	0	13	-1.384077	hypothetical protein
MUL_2543	1	15	-1.913593	hypothetical protein
MUL_2544	3	12	-0.467114	hypothetical protein
MUL_2545	3	14	-0.465653	ABC transporter ATP-binding protein
MUL_2546	3	14	-0.407256	transcriptional regulatory protein Whib-like
MUL_2547	4	17	-0.553702	ATP-dependent DNA helicase II UvrD2
MUL_2549	5	19	-1.397575	glutaredoxin protein
MUL_2550	3	16	-0.530323	transposase for IS2404

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_2542	PF06580	44	9e-07	Sensor histidine kinase
>PF06580#Sensor histidine kinase Length = 349 Score = 43.7 bits (103), Expect = 9e-07 Identities = 36/225 (16%), Positives = 87/225 (38%), Gaps = 40/225 (17%) Query: 282 EVKRRDRALISKDATIREIHHRVK-----NNLQTVAALLRLQARRTTNAEGREALTESVR 336 E+ + A ++++A + + ++ N L + AL+ + E +L+E +R Sbjct: 148 EIDQWKMASMAQEAQLMALKAQINPHFMFNALNNIRALILEDPTKA--REMLTSLSELMR 205 Query: 337 RVSSIALVHDALSMSVDEQVNLDEVIDRILPIMNDVASVDRPIRIN--RVGD-LGV---L 390 +L R + + +++ VD +++ + D L + Sbjct: 206 Y-------------------SLRYSNARQVSLADELTVVDSYLQLASIQFEDRLQFENQI 246 Query: 391 DSDRATALI--MVITELVQNAIEHAFDPAAQGA-VTIRAERSARWLDVVVHDDGRGLPSG 447 + + M++ LV+N I+H QG + ++ + + + V + G Sbjct: 247 NPAIMDVQVPPMLVQTLVENGIKHGIAQLPQGGKILLKGTKDNGTVTLEVENTGSLALK- 305 Query: 448 FSLEKSDSLGLQIVRTLVSAEL--DGSLGMREAPGRGTDVVLRVP 490 + ++S GLQ VR + + + + E G+ ++ +P Sbjct: 306 -NTKESTGTGLQNVRERLQMLYGTEAQIKLSEKQGKVN-AMVLIP 348
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_2545	adhesinb	29	0.010	Adhesin B signature.
>adhesinb#Adhesin B signature. Length = 310 Score = 29.4 bits (66), Expect = 0.010 Identities = 18/50 (36%), Positives = 22/50 (44%), Gaps = 8/50 (16%) Query: 130 VEALEALPDTEIKEALQALPEEFRMAV-------YYADVEGFPYKEIAEI 172 VE L AL D E KE +P E +M V Y++ P I EI Sbjct: 178 VEKLSAL-DKEAKEKFNNIPGEKKMIVTSEGCFKYFSKAYNVPSAYIWEI 226
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_2546	DHBDHDRGNASE	70	2e-16	2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase signature. Length = 261 Score = 70.5 bits (172), Expect = 2e-16 Identities = 50/191 (26%), Positives = 88/191 (46%), Gaps = 10/191 (5%) Query: 3 LNGKTMFISGASRGIGLAIAKRAAQDGANIALIAKTAEPHPKLPGTVYTAAKELEEAGGQ 62 + GK FI+GA++GIG A+A+ A GA+IA + E K+ ++ A+ E Sbjct: 6 IEGKIAFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHAE----- 60 Query: 63 ALPIVGDVRDPDSVSAAVAKTVEQFGGIDICVNNASAINLGSITEVPMKRFDLMNGIQVR 122 A P DVRD ++ A+ + G IDI VN A + G I + + ++ + Sbjct: 61 AFPA--DVRDSAAIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATFSVNST 118 Query: 123 GTYAVSQACIPHLKGRENPHILTL-SPPVQLDKKWLKPTAYMMAKFGMTLCALGIAEEMR 181 G + S++ ++ R + I+T+ S P + + AY +K + + E+ Sbjct: 119 GVFNASRSVSKYMMDRRSGSIVTVGSNPAGVPR--TSMAAYASSKAAAVMFTKCLGLELA 176 Query: 182 DEGIASNTLWP 192 + I N + P Sbjct: 177 EYNIRCNIVSP 187
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_2550	PF03544	32	0.004	Gram-negative bacterial tonB protein
>PF03544#Gram-negative bacterial tonB protein Length = 243 Score = 32.3 bits (73), Expect = 0.004 Identities = 18/97 (18%), Positives = 24/97 (24%), Gaps = 4/97 (4%) Query: 287 LPPVQPTKPPKANEVKIDPPAQAKP---PEQIVVPPGPDPVPAPADDWPVDEALPNP-TD 342 L P Q +PP V+ +P + P E VV P P P P P Sbjct: 60 LEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKPVKKVEQPKRDVK 119 Query: 343 MPVVPFAGSPQLPGNTLADSFAGRGGGTGLSAGAPKL 379 A + S + Sbjct: 120 PVESRPASPFENTAPARPTSSTATAATSKPVTSVASG 156 Score = 29.9 bits (67), Expect = 0.020 Identities = 20/109 (18%), Positives = 31/109 (28%), Gaps = 3/109 (2%) Query: 289 PVQPTKPPKANEVKIDPPAQAKPPEQIVVPPGPDPVPAP---ADDWPVDEALPNPTDMPV 345 P QP ++PP +PP + VV P P+P P P + V E Sbjct: 46 PAQPISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKP 105 Query: 346 VPFAGSPQLPGNTLADSFAGRGGGTGLSAGAPKLKPASFGGAGAASMRP 394 P Q + + P A+ + + Sbjct: 106 KPVKKVEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVA 154

MUL_2620

MUL_2625

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
MUL_2620	1	9	0.663753	preprotein translocase subunit SecA
MUL_2621	1	9	0.637643	hypothetical protein
MUL_2623	1	12	0.897118	hypothetical protein
MUL_2624	1	11	0.944415	hypothetical protein
MUL_2625	1	14	0.910538	lipoprotein LpqB

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_2620	HTHTETR	47	2e-08	TetR bacterial regulatory protein HTH signature.
>HTHTETR#TetR bacterial regulatory protein HTH signature. Length = 215 Score = 46.9 bits (111), Expect = 2e-08 Identities = 21/107 (19%), Positives = 38/107 (35%), Gaps = 3/107 (2%) Query: 16 GAASRAQTRHLLLTAAAEEFARVGYVASTVSRIAEGAGVTVQTLYLAWGSKRALLRGYLE 75 +TR +L A F++ G ++++ IA+ AGVT +Y + K L E Sbjct: 5 TKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSEIWE 64 Query: 76 ---STLAPDAAPSGQHFAAQLQPDSPAGTLAQVSALVCDAARRSAIA 119 S + F + + + V + RR + Sbjct: 65 LSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVTEERRRLLME 111
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_2623	HTHFIS	60	1e-13	FIS bacterial regulatory protein HTH signature.
>HTHFIS#FIS bacterial regulatory protein HTH signature. Length = 484 Score = 60.2 bits (146), Expect = 1e-13 Identities = 23/123 (18%), Positives = 52/123 (42%), Gaps = 11/123 (8%) Query: 10 ILLIEDDPGDELITREAFEHNKVNNRLHVAHDGEEGLDYLYQRGKYQQARRPDLILLDLN 69 IL+ +DD + +A + + + + ++ A DL++ D+ Sbjct: 6 ILVADDDAAIRTVLNQALS--RAGYDVRITSNAATLWRWI-------AAGDGDLVVTDVV 56 Query: 70 LPKYDGRQLLEKIKFDSELCRIPVVVLTTSSAEEDILRSYNLHANAYVTKPVDLDQFMTA 129 +P + LL +IK +PV+V++ + +++ A Y+ KP DL + + Sbjct: 57 MPDENAFDLLPRIK--KARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGI 114 Query: 130 VRQ 132 + + Sbjct: 115 IGR 117
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_2624	BCTERIALGSPH	30	0.023	Bacterial general secretion pathway protein H signa...
>BCTERIALGSPH#Bacterial general secretion pathway protein H signature. Length = 170 Score = 29.5 bits (66), Expect = 0.023 Identities = 19/61 (31%), Positives = 27/61 (44%), Gaps = 8/61 (13%) Query: 26 VLSIMGVMVLAGTVAGAVLLNRTDGVSRELSDNIEPARVAAFQLQ-----SALRDQESGI 80 +L +M +++L G AG VLL SR+ S AR A QL+ Q G+ Sbjct: 8 LLEMMLILLLMGVSAGMVLL--AFPASRDDSAAQTLARFEA-QLRFVQQRGLQTGQFFGV 64 Query: 81 R 81 Sbjct: 65 S 65
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_2625	HTHFIS	71	2e-15	FIS bacterial regulatory protein HTH signature.
>HTHFIS#FIS bacterial regulatory protein HTH signature. Length = 484 Score = 71.4 bits (175), Expect = 2e-15 Identities = 37/146 (25%), Positives = 64/146 (43%), Gaps = 4/146 (2%) Query: 27 SLLLVEDARADAMLVEELIADAAVDIQVVWARSMAHAERELSAARPDCVLLDLNLPDASG 86 ++L+ +D A ++ + ++ A D V + A R ++A D V+ D+ +PD + Sbjct: 5 TILVADDDAAIRTVLNQALSRAGYD--VRITSNAATLWRWIAAGDGDLVVTDVVMPDENA 62 Query: 87 IDALDRIANRDATVPVVVLTGLNDEYFGATAVAAGAQDYLVKGRVDPEM--LRRAMLYAI 144 D L RI +PV+V++ N A GA DYL K E+ + L Sbjct: 63 FDLLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAEP 122 Query: 145 ERKRAELIAADLHATQLRARENALLE 170 +R+ ++L L R A+ E Sbjct: 123 KRRPSKLEDDSQDGMPLVGRSAAMQE 148

MUL_2851

MUL_2857

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
MUL_2851	1	18	2.420888	mutator protein MutT3
MUL_2852	1	18	1.864808	hypothetical protein
MUL_2853	1	18	1.564694	glutamine-binding lipoprotein GlnH
MUL_2854	1	18	-2.810015	serine/threonine-protein kinase PknG
MUL_2855	3	21	-2.908095	hypothetical protein
MUL_2856	2	20	-2.155980	acetate kinase
MUL_2857	2	17	-1.096357	F420-dependent glucose-6-phosphate dehydrogenase

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_2851	NUCEPIMERASE	34	2e-04	Nucleotide sugar epimerase signature.
>NUCEPIMERASE#Nucleotide sugar epimerase signature. Length = 334 Score = 34.4 bits (79), Expect = 2e-04 Identities = 20/71 (28%), Positives = 32/71 (45%), Gaps = 12/71 (16%) Query: 60 GRLGRHLAAA----GHRVVGVDV-----DPALIEA--AEQDYPGPQWLVGDLAELDLPAR 108 G +G H++ GH+VVG+D D +L +A PG Q+ DLA+ + Sbjct: 10 GFIGFHVSKRLLEAGHQVVGIDNLNDYYDVSLKQARLELLAQPGFQFHKIDLADREGMTD 69 Query: 109 GIAE-PFDVIV 118 A F+ + Sbjct: 70 LFASGHFERVF 80
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_2853	cloacin	39	4e-05	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 38.5 bits (89), Expect = 4e-05 Identities = 32/102 (31%), Positives = 38/102 (37%) Query: 219 GNGGAGGDGGAGGVGGDGGAGGVGGVGGDGGAGGVGGVGGDGGWLIGDGGAGGQGGVGGM 278 G G G + GA G+ G G G G + G G + W G G GG G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 279 GGTGGAGGSGVAGAHGGNATSAVAAFGGDGGAGGDAGHGGTG 320 G GG G SG GGN ++ A A G GG Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 37.8 bits (87), Expect = 7e-05 Identities = 30/79 (37%), Positives = 36/79 (45%), Gaps = 2/79 (2%) Query: 163 GNAGLIGNGGAGGNGGA--GGNGGAGAAGGTGDNGGWLYGSGGDGGTGGNALVAGGTGGN 220 G G N GA G GG G G GG D GW + GG G+ + GG G+ Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 221 GGAGGDGGAGGVGGDGGAG 239 G GG+G +GG G GG Sbjct: 63 GNGGGNGNSGGGSGTGGNL 81 Score = 35.8 bits (82), Expect = 2e-04 Identities = 28/71 (39%), Positives = 36/71 (50%), Gaps = 1/71 (1%) Query: 125 GAAGTATNPNGGAGGLLYGNGGAGFNNGATAGAAGGNGGNAGLIGNGGAGGNGGAGGNGG 184 GA T+ N NGG GL G G + + ++ G G +G I GG G+G GGNG Sbjct: 12 GAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSG-IHWGGGSGHGNGGGNGN 70 Query: 185 AGAAGGTGDNG 195 +G GTG N Sbjct: 71 SGGGSGTGGNL 81 Score = 35.5 bits (81), Expect = 4e-04 Identities = 33/82 (40%), Positives = 38/82 (46%), Gaps = 2/82 (2%) Query: 143 GNGGAGFNNGATAGAAGGNGGNAGLIGNGGAGGNGGAGGNGGAGAAGGTGDNGGWLYGSG 202 G G G N GA + + NGG GL GGA G GG+G W GSG Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWG-GGSGSGIHWGGGSG 61 Query: 203 -GDGGTGGNALVAGGTGGNGGA 223 G+GG GN+ GTGGN A Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSA 83 Score = 33.5 bits (76), Expect = 0.001 Identities = 31/100 (31%), Positives = 37/100 (37%), Gaps = 5/100 (5%) Query: 159 GGNGGNAGLIGNGGAGGNGGAGGNGGAGAAGGTGDNGGWLYGSGGDGGTGGNALVAGGTG 218 G N G GN G G G G + +G + +N W GSG GG G Sbjct: 8 GHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGG-----GSGH 62 Query: 219 GNGGAGGDGGAGGVGGDGGAGGVGGVGGDGGAGGVGGVGG 258 GNGG G+ G G G + V A G GG Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG 102 Score = 33.1 bits (75), Expect = 0.002 Identities = 29/101 (28%), Positives = 36/101 (35%) Query: 266 DGGAGGQGGVGGMGGTGGAGGSGVAGAHGGNATSAVAAFGGDGGAGGDAGHGGTGGDGGN 325 + GA G G TG G G + G ++ + G G G G G G Sbjct: 10 NTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNG 69 Query: 326 GGQGASGGRGGLLSGAQGVTGTAGDGGTGGDGGLHGAFGAG 366 G SG G L + A V T G GGL + AG Sbjct: 70 NSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAG 110 Score = 31.2 bits (70), Expect = 0.008 Identities = 35/115 (30%), Positives = 45/115 (39%), Gaps = 4/115 (3%) Query: 190 GTGDNGGWLYGSGG-DGGTGGNALVAGGTGGNGGAGGDGGAGGVGGDGGAGGVGGVGGDG 248 G G N G SG +GG G + G + G+G + + GG G G G G G+G Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNG 65 Query: 249 GAGGVGGVGGDGGWLIGDGGAGGQGGVGGMGGTGGAGGSGVAGAHGGNATSAVAA 303 G G G G G G+ A G G G+A + A SA A Sbjct: 66 GGNGNSGGGSGTG---GNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAAIA 117
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_2855	IGASERPTASE	30	0.016	IgA-specific serine endopeptidase (S6) signature.
>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature. Length = 1541 Score = 30.0 bits (67), Expect = 0.016 Identities = 17/51 (33%), Positives = 21/51 (41%), Gaps = 3/51 (5%) Query: 283 VTFDEDRHRAHTGNGAQVLATLRNTAINLHRLNGADNIADACRITALTANR 333 V E+ H TGN L N I+ LN ADN + + LT N Sbjct: 846 VRLTENSHWHLTGNSDVHQLDLANGHIH---LNSADNSNNVTKYNTLTVNS 893
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_2856	TCRTETA	29	0.003	Tetracycline resistance protein signature.
>TCRTETA#Tetracycline resistance protein signature. Length = 399 Score = 29.0 bits (65), Expect = 0.003 Identities = 12/100 (12%), Positives = 33/100 (33%), Gaps = 4/100 (4%) Query: 1 MPTTTPPRRRPTEGIAQMNIVPASIEEKISRVDRQRSLAIGISCGVLAAWSFFRLVWLLY 60 +P + RRP + + + +R + + + + +W+++ Sbjct: 181 LPESHKGERRP----LRREALNPLASFRWARGMTVVAALMAVFFIMQLVGQVPAALWVIF 236 Query: 61 VSMPFGWFMGAVAFQFVLWAVVGSVATNAAAGFLARYFKD 100 F W + + ++ S+A G +A + Sbjct: 237 GEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGE 276
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_2857	TCRTETOQM	26	0.014	Tetracycline resistance protein TetO/TetQ/TetM family ...
>TCRTETOQM#Tetracycline resistance protein TetO/TetQ/TetM family signature. Length = 639 Score = 26.4 bits (58), Expect = 0.014 Identities = 11/44 (25%), Positives = 22/44 (50%), Gaps = 4/44 (9%) Query: 22 RVEHGELRVGDEVRINDGSGVRVDAIEAF----RKKLDTAKAGD 61 R+ G L + D VRI++ +++ + K+D A +G+ Sbjct: 269 RLYSGVLHLRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGE 312

MUL_3007

MUL_3017

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
MUL_3007	-2	9	0.099859	hypothetical protein
MUL_3008	-1	11	0.264153	dehydrogenase
MUL_3010	-1	11	-0.345410	NADP-dependent alcohol dehydrogenase AdhC
MUL_3011	-2	11	0.134237	hypothetical protein
MUL_3012	-1	11	0.680174	hypothetical protein
MUL_3013	-2	13	1.165621	hypothetical protein
MUL_3015	-1	11	0.709880	transposase for IS2404
MUL_3016	-1	10	-0.542761	secreted antigen 85-B FbpB
MUL_3017	-1	11	0.047411	hypothetical protein

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_3007	NUCEPIMERASE	47	1e-07	Nucleotide sugar epimerase signature.
>NUCEPIMERASE#Nucleotide sugar epimerase signature. Length = 334 Score = 46.7 bits (111), Expect = 1e-07 Identities = 23/84 (27%), Positives = 33/84 (39%), Gaps = 17/84 (20%) Query: 1 MHVLVTDAAGAIGRLVTRQLIAAGHTVSGISSHPHDYLDP-------------NVDFVCA 47 M LVT AAG IG V+++L+ AGH V GI + +DY D F Sbjct: 1 MKYLVTGAAGFIGFHVSKRLLEAGHQVVGIDNL-NDYYDVSLKQARLELLAQPGFQFHKI 59 Query: 48 SLRNPVLLEL---ASEADAVIHLA 68 L + + + + V Sbjct: 60 DLADREGMTDLFASGHFERVFISP 83
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_3010	SACTRNSFRASE	35	1e-05	Streptothricin acetyltransferase signature.
>SACTRNSFRASE#Streptothricin acetyltransferase signature. Length = 173 Score = 35.3 bits (81), Expect = 1e-05 Identities = 15/58 (25%), Positives = 20/58 (34%), Gaps = 4/58 (6%) Query: 31 IYVDPEHVCTGVGRLLMTAARERLWRVGVTAAVLW--VLDGNARARRFYERDGWNFDG 86 I V ++ GVG L+ A E W L D N A FY + + Sbjct: 95 IAVAKDYRKKGVGTALLHKAIE--WAKENHFCGLMLETQDINISACHFYAKHHFIIGA 150
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_3011	DHBDHDRGNASE	97	3e-26	2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase signature. Length = 261 Score = 97.4 bits (242), Expect = 3e-26 Identities = 66/236 (27%), Positives = 107/236 (45%), Gaps = 23/236 (9%) Query: 11 VRNKVIVITGGARGIGLATATALHKLGAKVAIGDVGEPAVKEAGADLGLEVYG----KLD 66 + K+ ITG A+GIG A A L GA +A D +++ + L E D Sbjct: 6 IEGKIAFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHAEAFPAD 65 Query: 67 VTDPNSFSDFLDQVERQLGPLDVLVNNAGIMPVGRIVDEPDSVTRRILDINVYGVMVGSK 126 V D + + ++ER++GP+D+LVN AG++ G I D +N GV S+ Sbjct: 66 VRDSAAIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGVFNASR 125 Query: 127 LAAQRMVPRGRGHVINVASLAGEIYVVGLATYCASKHAVIAFTDAARIEYRTTGVKFSMV 186 ++ M+ R G ++ V S + +A Y +SK A + FT +E ++ ++V Sbjct: 126 SVSKYMMDRRSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLELAEYNIRCNIV 185 Query: 187 LPTFVNTELA--------SGTPGMKGF-----------KNAEPSDIADAIVALVAN 223 P T++ +KG K A+PSDIADA++ LV+ Sbjct: 186 SPGSTETDMQWSLWADENGAEQVIKGSLETFKTGIPLKKLAKPSDIADAVLFLVSG 241
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_3016	ACRIFLAVINRP	26	0.019	Acriflavin resistance protein family signature.
>ACRIFLAVINRP#Acriflavin resistance protein family signature. Length = 1034 Score = 26.3 bits (58), Expect = 0.019 Identities = 14/42 (33%), Positives = 22/42 (52%) Query: 24 IGALAGWIAGKIVKGAGSGILMNIVIGVVGALIGGFLLSFFV 65 + + G + I GAGSG + IGV+G ++ LL+ F Sbjct: 979 LAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIFF 1020
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_3017	GPOSANCHOR	34	6e-04	Gram-positive coccus surface protein anchor signature.
>GPOSANCHOR#Gram-positive coccus surface protein anchor signature. Length = 539 Score = 34.3 bits (78), Expect = 6e-04 Identities = 14/60 (23%), Positives = 16/60 (26%), Gaps = 4/60 (6%) Query: 247 AKVLAESIRPWMPPPAPAPAPAPGEPAPQPGAPEPVPAPAPAPAAGVAPTVAPAPRTQPT 306 AK L E + A A A P+ P P G AP P Sbjct: 441 AKALKEK----LAKQAEELAKLRAGKASDSQTPDAKPGNKAVPGKGQAPQAGTKPNQNKA 496

MUL_3154

MUL_3162

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
MUL_3154	0	11	-0.597523	hypothetical protein
MUL_3155	-1	10	-0.823418	glycosyl transferase family protein
MUL_3156	-1	13	-1.505524	hypothetical protein
MUL_3157	0	12	-1.365701	cytochrome P450 143A3 Cyp143A3
MUL_3160	1	12	-0.826187	hypothetical protein
MUL_3161	3	11	-0.708309	short-chain type dehydrogenase/reductase
MUL_3162	3	11	-0.516071	hypothetical protein

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_3154	RTXTOXINA	31	0.006	Gram-negative bacterial RTX toxin determinant A family...
>RTXTOXINA#Gram-negative bacterial RTX toxin determinant A family signature. Length = 1024 Score = 31.1 bits (70), Expect = 0.006 Identities = 17/60 (28%), Positives = 25/60 (41%), Gaps = 6/60 (10%) Query: 152 TGLLVIMDANPAASQGSLLAAP------DVMGLIDAIRQRGKVIVIDPVRTVTAARADEW 205 T L + AA+ SL+ AP V G+I I + K + + V + A EW Sbjct: 373 TVLASVSSGISAAATTSLVGAPVSALVGAVTGIISGILEASKQAMFEHVASKMADVIAEW 432
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_3155	TCRTETA	51	5e-09	Tetracycline resistance protein signature.
>TCRTETA#Tetracycline resistance protein signature. Length = 399 Score = 51.0 bits (122), Expect = 5e-09 Identities = 40/184 (21%), Positives = 71/184 (38%), Gaps = 4/184 (2%) Query: 23 RVIVLLALVVGLEGASNGTIGALAVVLKQAFGITNLQV---GLLVTASTAIGIVVMLVSG 79 R ++++ V L+ G I + L + +N G+L+ + V G Sbjct: 5 RPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHYGILLALYALMQFACAPVLG 64 Query: 80 TLADRVNRTRVLWITVLIWSVAMALGGISAGYGWLLASRVALGAVVAVGGPVVASLMGDF 139 L+DR R VL +++ +V A+ + L R+ + + G V + + D Sbjct: 65 ALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIGRI-VAGITGATGAVAGAYIADI 123 Query: 140 FAQHERGRIYGFVLAGEGICTALGVLVSGWLAAITWRLSFLWLAVTGLLLTLALARTVPE 199 ER R +GF+ A G G ++ G + + F A L L +PE Sbjct: 124 TDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPFFAAAALNGLNFLTGCFLLPE 183 Query: 200 PARG 203 +G Sbjct: 184 SHKG 187
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_3160	DHBDHDRGNASE	81	4e-20	2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase signature. Length = 261 Score = 80.9 bits (199), Expect = 4e-20 Identities = 50/194 (25%), Positives = 86/194 (44%), Gaps = 1/194 (0%) Query: 9 NTSDLAGRVVAITGAGSGIGRELALLCAQRGADLALCDINDTAVADTAQTARGFGHDVIT 68 N + G++ ITGA GIG +A A +GA +A D N + + + Sbjct: 2 NAKGIEGKIAFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHAEA 61 Query: 69 RRVDVSDPEQMTAFADATLGHFGGVDLLVNNAGVGLIGGFLDTSRKDWDWLVSINVMGVV 128 DV D + G +D+LVN AGV G S ++W+ S+N GV Sbjct: 62 FPADVRDSAAIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGVF 121 Query: 129 HGCEAFLPAMIESGRGGHVVNLSSAAGLLANPALSAYSATKFAVLGLSEALRIELEPHRI 188 + + M++ R G +V + S + +++AY+++K A + ++ L +EL + I Sbjct: 122 NASRSVSKYMMDR-RSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLELAEYNI 180 Query: 189 GVTAICPGVINTAI 202 + PG T + Sbjct: 181 RCNIVSPGSTETDM 194
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_3162	CHANLCOLICIN	29	0.030	Channel forming colicin signature.
>CHANLCOLICIN#Channel forming colicin signature. Length = 522 Score = 29.3 bits (65), Expect = 0.030 Identities = 22/77 (28%), Positives = 37/77 (48%), Gaps = 7/77 (9%) Query: 144 ASPTLLNHTAKSAARAYANMELPLAEVKAVAKATDTSINDVVMTIVDDALHHYLDEHRAP 203 ++ L A+ AARA A AE +A AKA ++ + IV++AL H + R P Sbjct: 58 STAQLKKTQAEQAARAKA-----AAEAQAKAKANRDALTQRLKDIVNEALRH--NASRTP 110 Query: 204 ADGPLVALMPMSMRSQA 220 + L +M+++ Sbjct: 111 SATELAHANNAAMQAED 127

MUL_3375

MUL_3382

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
MUL_3375	0	10	1.001166	enoyl-CoA hydratase
MUL_3376	0	11	0.792435	hypothetical protein
MUL_3377	-1	11	0.037327	hypothetical protein
MUL_3378	0	12	1.308914	transposase for IS2404
MUL_3379	1	13	0.588165	PE-PGRS family protein
MUL_3380	0	13	0.884691	non-IS element not present in Mycobacterium
MUL_3381	2	13	1.886096	non-IS element not present in Mycobacterium
MUL_3382	2	16	1.959443	non-IS element not present in Mycobacterium

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_3375	PERTACTIN	35	8e-04	Pertactin signature.
>PERTACTIN#Pertactin signature. Length = 922 Score = 35.5 bits (81), Expect = 8e-04 Identities = 27/87 (31%), Positives = 36/87 (41%), Gaps = 9/87 (10%) Query: 352 LFDASTSSWGDLTAAPPPQAPPAPLTPPTPPVPPTPPQPPKQTSDT-SPSGPGPHFLAAS 410 L A P PQ P P PP PP PP PPQPP++ + +P P L+A+ Sbjct: 562 LVGAKAPPAPKPAPQPGPQPGPQPPQPPQPPQPPQPPQPPQRQPEAPAPQPPAGRELSAA 621 Query: 411 LRNPWMLLGAAALVALIVFAAQGIWLS 437 AA+ V A +W + Sbjct: 622 --------ANAAVNTGGVGLASTLWYA 640
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_3376	AUTOINDCRSYN	30	0.016	Autoinducer synthesis protein signature.
>AUTOINDCRSYN#Autoinducer synthesis protein signature. Length = 216 Score = 29.8 bits (67), Expect = 0.016 Identities = 15/88 (17%), Positives = 30/88 (34%), Gaps = 11/88 (12%) Query: 49 SDPHRFGRVDDDGTVWLITTAGERIVGSWQAGDA------EAAFAHFGRRFDDLNTEITL 102 +D F + D++ T +L ++ S + + F + F ++N Sbjct: 39 TDGMEFDQYDNNNTTYLFGIKDNTVICSLRFIETKYPNMITGTFFPY---FKEINIPEGN 95 Query: 103 MEE--RLAAGTGDARKIRANAAALAETL 128 E R A+ I N ++ L Sbjct: 96 YLESSRFFVDKSRAKDILGNEYPISSML 123
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_3381	2FE2SRDCTASE	33	5e-04	Ferric iron reductase signature.
>2FE2SRDCTASE#Ferric iron reductase signature. Length = 262 Score = 33.5 bits (76), Expect = 5e-04 Identities = 11/21 (52%), Positives = 13/21 (61%) Query: 222 RNSCCLYYRLPGAGKRGDCPL 242 R +CC YRLP + GDC L Sbjct: 241 RRTCCQRYRLPDVQQCGDCTL 261
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_3382	PF06580	24	0.039	Sensor histidine kinase
>PF06580#Sensor histidine kinase Length = 349 Score = 24.4 bits (53), Expect = 0.039 Identities = 5/23 (21%), Positives = 9/23 (39%) Query: 4 GVRLTEFHERITLRFGAAYGASV 26 G L ER+ + +G + Sbjct: 312 GTGLQNVRERLQMLYGTEAQIKL 334

MUL_3386

MUL_3401

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
MUL_3386	0	10	-0.465338	arsenic-transport integral membrane protein
MUL_3387	0	11	-0.085038	antibiotic ABC transporter integral membrane
MUL_3388	-1	11	-0.058860	SAM-dependent methyltransferase
MUL_3389	0	12	0.023250	integral membrane alanine, valine and leucine
MUL_3390	-2	10	-0.123585	TRK system potassium uptake protein CeoB
MUL_3391	-2	17	-1.208061	TRK system potassium uptake protein CeoC
MUL_3392	-1	16	-1.171687	integral membrane alanine and leucine rich
MUL_3393	0	14	-1.500205	hypothetical protein
MUL_3395	0	13	-1.985633	hypothetical protein
MUL_3396	-2	11	-1.705199	hypothetical protein
MUL_3398	-1	12	-0.772277	deoxyuridine 5'-triphosphate
MUL_3399	-1	13	-0.716495	hypothetical protein
MUL_3400	-1	11	-0.551045	hypothetical protein
MUL_3401	0	11	-0.185381	hypothetical protein

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_3386	RTXTOXIND	28	0.034	Gram-negative bacterial RTX secretion protein D signat...
>RTXTOXIND#Gram-negative bacterial RTX secretion protein D signature. Length = 478 Score = 28.3 bits (63), Expect = 0.034 Identities = 19/168 (11%), Positives = 52/168 (30%), Gaps = 24/168 (14%) Query: 14 ALFNSKIDEHADPKVQIQQAIEEAQRTHQALTQQAAQVIGNQRQLEMRLNRQLADIEKLQ 73 +L + + K Q + +++ + + A++ + + +L D L Sbjct: 189 SLIKEQFSTWQNQKYQKELNLDKKRAERLTVL---ARINRYENLSRV-EKSRLDDFSSL- 243 Query: 74 VNARQALTLADQATAAGDAAKAVEYDNAAEAFAAQLVTAEQSVEDLKALHDQALNAAAQA 133 K +A + V A + K+ +Q + A Sbjct: 244 ------------------LHKQAIAKHAVLEQENKYVEAVNELRVYKSQLEQIESEILSA 285 Query: 134 KRAVEQNSMVLQQKIA-ERAKLLSQLEQAKMQEQVSSSLRSMSELAAP 180 K + + + + +I + + + ++ + + S + AP Sbjct: 286 KEEYQLVTQLFKNEILDKLRQTTDNIGLLTLELAKNEERQQASVIRAP 333
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_3389	SACTRNSFRASE	39	3e-06	Streptothricin acetyltransferase signature.
>SACTRNSFRASE#Streptothricin acetyltransferase signature. Length = 173 Score = 38.8 bits (90), Expect = 3e-06 Identities = 17/76 (22%), Positives = 32/76 (42%), Gaps = 5/76 (6%) Query: 58 IQGNVIGCGALHVLWSDLGEVRTVAVDPAMTGHGIGHAIVDRLLEVARELQLERLFVLTF 117 ++ N IG + W+ + +AV G+G A++ + +E A+E L + T Sbjct: 72 LENNCIGRIKIRSNWNGYALIEDIAVAKDYRKKGVGTALLHKAIEWAKENHFCGLMLETQ 131 Query: 118 ET-----EFFTAHGFT 128 + F+ H F Sbjct: 132 DINISACHFYAKHHFI 147
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_3390	PREPILNPTASE	31	0.016	Type IV prepilin cysteine protease (C20) family sig...
>PREPILNPTASE#Type IV prepilin cysteine protease (C20) family signature. Length = 290 Score = 31.3 bits (71), Expect = 0.016 Identities = 28/100 (28%), Positives = 41/100 (41%), Gaps = 8/100 (8%) Query: 167 PIVLAGTAI----VLMRTESNPDTRPRLILGSSLIALSFLGLRHLWAGSPESPELRQRAA 222 P+V TA+ V M T L+L L+AL+F+ L + P+ L Sbjct: 111 PLVELLTALLSVAVAMTLAPGWGTLAALLLTWVLVALTFIDLDKMLL--PDQLTLPLLWG 168 Query: 223 GFIGFTIGGPLSDGLTVWIAAP--LLFIGALFGLLLLTGT 260 G + +GG +S G V A L+ + LLTG Sbjct: 169 GLLFNLLGGFVSLGDAVIGAMAGYLVLWSLYWAFKLLTGK 208
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_3392	DHBDHDRGNASE	85	8e-22	2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase signature. Length = 261 Score = 85.5 bits (211), Expect = 8e-22 Identities = 82/269 (30%), Positives = 122/269 (45%), Gaps = 23/269 (8%) Query: 9 LSGRVAFITGAARGQGRAHALRLARDGADVIAVDLCDQIASVPYPLGTAEELATTVKLVE 68 + G++AFITGAA+G G A A LA GA + AVD E+L V ++ Sbjct: 6 IEGKIAFITGAAQGIGEAVARTLASQGAHIAAVDY------------NPEKLEKVVSSLK 53 Query: 69 DTGARIVASQADVRDREALAAALQAGIDELGQVDIVVANAGIAPM----QSGDDGWRDVI 124 A ADVRD A+ E+G +DI+V AG+ D+ W Sbjct: 54 AEARHAEAFPADVRDSAAIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATF 113 Query: 125 DVNLSGAYYTVEVAIPTMIEQGRGGSIVLISSAAGLVGISSADAGAIGYVASKHALVGLM 184 VN +G + M+++ R GSIV + S V +S A Y +SK A V Sbjct: 114 SVNSTGVFNASRSVSKYMMDR-RSGSIVTVGSNPAGVPRTSMAA----YASSKAAAVMFT 168 Query: 185 RVYANLLAPHSIRVNSLHPSGVDPPMINNEFIRHWLADLVAETGSGPGAGNALPV-QILQ 243 + LA ++IR N + P + M + + A+ V + GS +P+ ++ + Sbjct: 169 KCLGLELAEYNIRCNIVSPGSTETDMQWSLWADENGAEQVIK-GSLETFKTGIPLKKLAK 227 Query: 244 ADDIAGALAWLVSDEARYITGVALPVDAG 272 DIA A+ +LVS +A +IT L VD G Sbjct: 228 PSDIADAVLFLVSGQAGHITMHNLCVDGG 256
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_3399	HTHTETR	75	9e-19	TetR bacterial regulatory protein HTH signature.
>HTHTETR#TetR bacterial regulatory protein HTH signature. Length = 215 Score = 75.0 bits (184), Expect = 9e-19 Identities = 28/130 (21%), Positives = 51/130 (39%), Gaps = 4/130 (3%) Query: 34 ARQERGDAARNRELLLQAARRLVAKRGAEAVTTDDIAAEAGVGKGTLFRRFGSRAGLMMV 93 AR+ + +A R+ +L A RL +++G + + +IA AGV +G ++ F ++ L Sbjct: 2 ARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSE 61 Query: 94 LLDEDERASQQAF--LFGPPPLGPEAAPLDRLIAFGRERICFVHAHHELLSEANRNPLTR 151 + + E + P P + + LI LL E + Sbjct: 62 IWELSESNIGELELEYQAKFPGDPLSVLREILIHVLES--TVTEERRRLLMEIIFHKCEF 119 Query: 152 YGAAASVHRR 161 G A V + Sbjct: 120 VGEMAVVQQA 129
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_3400	DHBDHDRGNASE	94	1e-24	2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase signature. Length = 261 Score = 93.6 bits (232), Expect = 1e-24 Identities = 59/189 (31%), Positives = 92/189 (48%), Gaps = 2/189 (1%) Query: 9 GKRCLVTGAASGIGRATALRLAEQGAELYLTDRDGDGLAQTVSAARALGAQVPEHRVLDI 68 GK +TGAA GIG A A LA QGA + D + + L + VS+ +A A+ E D+ Sbjct: 8 GKIAFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAE-ARHAEAFPADV 66 Query: 69 SDYDEVAAFAADIHASHPSMDVVLNIAGISAWGTVDRLSHEHWSKMVAVNLMGPIHVIET 128 D + A I +D+++N+AG+ G + LS E W +VN G + + Sbjct: 67 RDSAAIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGVFNASRS 126 Query: 129 FVPPMVAAGRGGHLVNVSSAAGLVALPWHAAYSASKYGLRGLSEVLRFDLARHRIGVSVV 188 M+ R G +V V S V AAY++SK ++ L +LA + I ++V Sbjct: 127 VSKYMMDR-RSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLELAEYNIRCNIV 185 Query: 189 VPGAVDTPL 197 PG+ +T + Sbjct: 186 SPGSTETDM 194
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_3401	HTHTETR	69	1e-16	TetR bacterial regulatory protein HTH signature.
>HTHTETR#TetR bacterial regulatory protein HTH signature. Length = 215 Score = 69.3 bits (169), Expect = 1e-16 Identities = 21/147 (14%), Positives = 53/147 (36%), Gaps = 5/147 (3%) Query: 17 RRRGDKQRQAILQAVRELLEERPFAELSVATISNRAGVARSGFYFYFDSKYSVLAQLMAE 76 ++ + RQ IL L ++ + S+ I+ AGV R Y++F K + +++ Sbjct: 6 KQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSEIWEL 65 Query: 77 AVEELEERTQYFAPRQPGESPQEFAKRM--VGSAAIVYTHNDPVMMACN--AARHTDIEI 132 + + E + + PG+ + + V + + +M ++ + Sbjct: 66 SESNIGELELEYQAKFPGDPLSVLREILIHVLESTVTEERRRLLMEIIFHKCEFVGEMAV 125 Query: 133 RDILDQQFDVVLRE-IVGVIDAEMRAG 158 + + + I + + A Sbjct: 126 VQQAQRNLCLESYDRIEQTLKHCIEAK 152

MUL_3419

MUL_3426

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
MUL_3419	-1	17	-0.079831	hypothetical protein
MUL_3422	0	16	0.765762	hypothetical protein
MUL_3423	-1	16	0.324634	transcriptional regulator NrdR
MUL_3424	2	14	-0.478413	hypothetical protein
MUL_3425	2	13	-0.384963	LexA repressor
MUL_3426	1	12	-0.366080	hypothetical protein

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_3419	IGASERPTASE	32	0.005	IgA-specific serine endopeptidase (S6) signature.
>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature. Length = 1541 Score = 31.6 bits (71), Expect = 0.005 Identities = 17/51 (33%), Positives = 20/51 (39%), Gaps = 3/51 (5%) Query: 283 VTFDEDRHRAHTGNGAQVLATLRNTAINLHRLNGADNIAEARRITALTANR 333 V E+ H TGN L N I+ LN ADN + LT N Sbjct: 846 VRLTENSHWHLTGNSDVHQLDLANGHIH---LNSADNSNNVTKYNTLTVNS 893
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_3422	SHAPEPROTEIN	68	1e-14	Bacterial cell shape determinant MreB/Mbl protein s...
>SHAPEPROTEIN#Bacterial cell shape determinant MreB/Mbl protein signature. Length = 347 Score = 68.2 bits (167), Expect = 1e-14 Identities = 82/376 (21%), Positives = 141/376 (37%), Gaps = 68/376 (18%) Query: 3 VGIDFGTTHTVAAVVDRGNYPVVSFDGVDAWPSAIAANAAGE------LRFGLDATA-VR 55 + ID GT +T+ V +G V PS +A G DA + Sbjct: 13 LSIDLGTANTLIYVKGQGI--------VLNEPSVVAIRQDRAGSPKSVAAVGHDAKQMLG 64 Query: 56 RDPGWSVLRSFKRLLNDAGPHTEVSLAGRSYRLTELLARFLEQLKDDLQHRSNAGLTPGE 115 R PG R + D G + + ++L F++Q+ SN+ + P Sbjct: 65 RTPGNIAA---IRPMKD-GVIADFFVT------EKMLQHFIKQVH------SNSFMRPSP 108 Query: 116 PVEAAISVPANASSAQRFLTLDAFVAAGFQVVALLNEPSAASLEYAHRYRSTITAKSEYV 175 V + VP A+ +R ++ AG + V L+ EP AA++ +++ Sbjct: 109 RV--LVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLP-----VSEATGS 161 Query: 176 VIYDLGGGTFDASLLKMTGHINDVVRSEGIQRLGGDDFDEAILQLVAARLP-EIAELAAT 234 ++ D+GGGT + +++ + G VV S + R+GGD FDEAI+ V I E A Sbjct: 162 MVVDIGGGTTEVAVISLNG----VVYSSSV-RIGGDRFDEAIINYVRRNYGSLIGEATAE 216 Query: 235 DVTGYDVLREECAARKEAVG---PQTRRFLMDLTGI----GGDRPPFSCDIDDVYSACAP 287 + K +G P +++ G G R F+ + +++ A Sbjct: 217 RI-------------KHEIGSAYPGDEVREIEVRGRNLAEGVPR-GFTLNSNEILEALQE 262 Query: 288 LVDDTIGVLSRVLRDPAPGGDGVAWSEVAGIYLAGGAGSFPLISRMLRATFGDKRVKRSP 347 + + + L P + + G+ L GG + R+L G V + Sbjct: 263 PLTGIVSAVMVALEQCPP--ELASDISERGMVLTGGGALLRNLDRLLMEETG-IPVVVAE 319 Query: 348 HAFAATAIGLAVFLDH 363 A G L+ Sbjct: 320 DPLTCVARGGGKALEM 335
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_3423	DHBDHDRGNASE	91	7e-24	2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase signature. Length = 261 Score = 91.3 bits (226), Expect = 7e-24 Identities = 50/185 (27%), Positives = 74/185 (40%), Gaps = 9/185 (4%) Query: 5 LITGCSTGLGRALAEAVIDAGHHTVATARSVGGVADLAQ------RSPERVLPLALDITE 58 ITG + G+G A+A + G H A + + + R E D+ + Sbjct: 12 FITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHAEA---FPADVRD 68 Query: 59 PDQITAAMQAAQQRFGGIDVLVNNAGYGYRAAVEEGDDAEVRDLFETHFFGTVALIKAVL 118 I ++ G ID+LVN AG + D E F + G ++V Sbjct: 69 SAAIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGVFNASRSVS 128 Query: 119 PDMRARRSGAIVNISSIAVALTPVGSGYYAAAKAAMEGMSGALHGELAPLGISVTVVEPG 178 M RRSG+IV + S + YA++KAA + L ELA I +V PG Sbjct: 129 KYMMDRRSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLELAEYNIRCNIVSPG 188 Query: 179 AFRTD 183 + TD Sbjct: 189 STETD 193
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_3426	HTHTETR	45	2e-08	TetR bacterial regulatory protein HTH signature.
>HTHTETR#TetR bacterial regulatory protein HTH signature. Length = 215 Score = 45.4 bits (107), Expect = 2e-08 Identities = 21/132 (15%), Positives = 51/132 (38%), Gaps = 5/132 (3%) Query: 1 MTREVERRPRDPAGRRQTIIEAAGRLIARHGLGDLTHRRVAAEADVPVGSTTYYFSDLGE 60 M R+ ++ ++ RQ I++ A RL ++ G+ + +A A V G+ ++F D + Sbjct: 1 MARKTKQEAQE---TRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSD 57 Query: 61 LREAALAHVATSATDWLEH-WERDLDESTDIP-ATLARLTADYLTDPDRHRTLNELYVAA 118 L ++ + + + + L + +T+ R + ++ Sbjct: 58 LFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVTEERRRLLMEIIFHKC 117 Query: 119 SHQPELQSLAQL 130 E+ + Q Sbjct: 118 EFVGEMAVVQQA 129

MUL_3502

MUL_3509

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
MUL_3502	2	13	2.917161	fatty-acid-CoA ligase
MUL_3503	1	11	2.795218	hypothetical protein
MUL_3504	1	13	3.117599	hypothetical protein
MUL_3505	1	12	3.488348	oxidoreductase GMC-type
MUL_3506	2	12	3.055198	transposase for IS2404
MUL_3507	3	12	2.750768	transposase for IS2606
MUL_3508	1	10	1.477949	hypothetical protein
MUL_3509	1	12	1.670757	*phosphoglucomutase

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_3502	PF03544	33	0.001	Gram-negative bacterial tonB protein
>PF03544#Gram-negative bacterial tonB protein Length = 243 Score = 33.4 bits (76), Expect = 0.001 Identities = 27/149 (18%), Positives = 51/149 (34%), Gaps = 7/149 (4%) Query: 387 FINIGYVIGLLPVTGLQLPLISAGGTSTATTLAMIGIIANAARHEPEAVAALRAGRDDRV 446 I+ V GLL + Q+ + A + T+ +A A P+AV + Sbjct: 23 CIHGAVVAGLLYTSVHQVIELPAPAQPISVTM-----VAPADLEPPQAVQPPPEPVVEPE 77 Query: 447 NRMLRLPLPKPYAPTRLEVFRDRKRVQPPAARPPAKQAAARKAPKAATRLAEEPLRPALP 506 +P P AP +E + + + +P + + K ++ E PA P Sbjct: 78 PEPEPIPEPPKEAPVVIEKPKPKPKPKPKPVKKVEQPKRDVKPVESRPASPFENTAPARP 137 Query: 507 RRPDRSGARSGQQGAGQRYAGQRHSGRVR 535 + + + +G R R + Sbjct: 138 --TSSTATAATSKPVTSVASGPRALSRNQ 164
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_3507	cloacin	41	1e-05	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 40.9 bits (95), Expect = 1e-05 Identities = 39/119 (32%), Positives = 49/119 (41%), Gaps = 17/119 (14%) Query: 408 SGGSGGIGGTGGHSLINSGGGIGGKGGGGGAAGLIGDGGAGGAGGNGGDGTGAGGLGGNG 467 SGG G TG HS + G I G G G G G + G+G GG+G Sbjct: 2 SGGDGRGHNTGAHS---TSGNINGGPTGLG-------VGGGASDGSGWSSENNPWGGGSG 51 Query: 468 ASADWIGNGGSGGAGGSGGLGAHAGAGGNGGSLYGNDGSG-------GAGGTGASGAGG 519 + W G G G GG+G G +G GGN ++ G GAGG S + G Sbjct: 52 SGIHWGGGSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAG 110 Score = 37.4 bits (86), Expect = 2e-04 Identities = 30/78 (38%), Positives = 31/78 (39%), Gaps = 1/78 (1%) Query: 160 GGNGGAAGLIGNGGAGGSGWAGGAGGAGGNGGWLYGNGGAGGLGGAAAGDYTAGGVGGAG 219 G N GA GN GG G GGA GW N GG G+ G G G Sbjct: 8 GHNTGAHSTSGNIN-GGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGG 66 Query: 220 GNAGLWGDGGAGGNGSAT 237 GN G G GGN SA Sbjct: 67 GNGNSGGGSGTGGNLSAV 84 Score = 37.0 bits (85), Expect = 2e-04 Identities = 29/82 (35%), Positives = 37/82 (45%), Gaps = 1/82 (1%) Query: 446 GAGGAGGNGGDGTGAGGLGGNGASADWIGNGGSGGAGGSGGLGAHAGAGGNGGSLYGNDG 505 G G G N G + +G + G G + +G G S G+G S G G+G G G Sbjct: 3 GGDGRGHNTGAHSTSGNING-GPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 506 SGGAGGTGASGAGGNGGGGGAA 527 G GG G SG G GG +A Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSA 83 Score = 35.8 bits (82), Expect = 4e-04 Identities = 27/78 (34%), Positives = 35/78 (44%), Gaps = 6/78 (7%) Query: 483 GSGGLGAHAGAGGNGGSLYGNDGSGGAGGTGASGAG------GNGGGGGAAGMMGDGGAG 536 G G G + GA G++ G G GG + G+G GGG G+ G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 537 GDGGDGSAAGGLGGDGGN 554 G+GG +GG G GGN Sbjct: 63 GNGGGNGNSGGGSGTGGN 80 Score = 35.5 bits (81), Expect = 6e-04 Identities = 29/88 (32%), Positives = 37/88 (42%), Gaps = 4/88 (4%) Query: 458 TGAGGLGGNGASADWIGNGGSGGAGGSGGLGAHAGAGGNGGSLYGNDGSGGAGGTGASGA 517 +G G G N + GN G G G GA G+G + + GSG G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 518 GGNGGGGGAAGMMGDGGAGGDGGDGSAA 545 GNGGG G +G GG+G G + A Sbjct: 62 HGNGGGNGNSG----GGSGTGGNLSAVA 85 Score = 34.7 bits (79), Expect = 0.001 Identities = 33/98 (33%), Positives = 41/98 (41%), Gaps = 5/98 (5%) Query: 125 GAAGTAASPNGGAGGLLYGNGGAGYSYTSGATAEAGGNGGAAGLIGNGGAGGSGWAGGAG 184 GA T+ + NGG GL G G S SG ++E GG +G GG G G Sbjct: 12 GAHSTSGNINGGPTGL---GVGGGASDGSGWSSENNPWGGGSG--SGIHWGGGSGHGNGG 66 Query: 185 GAGGNGGWLYGNGGAGGLGGAAAGDYTAGGVGGAGGNA 222 G G +GG G + A + A GAGG A Sbjct: 67 GNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 32.8 bits (74), Expect = 0.004 Identities = 33/86 (38%), Positives = 36/86 (41%), Gaps = 6/86 (6%) Query: 369 SGLIGDGGAGGAGGTGGGNAGVGGVGGVGGTGGAARLFGSGGSGGIGGTGGHSLINSGGG 428 SG G G GA T G G G G+G GGA+ G G G S I+ GGG Sbjct: 2 SGGDGRGHNTGAHSTSGNING--GPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGG 59 Query: 429 IGGKGGGGGAAGLIGDGGAGGAGGNG 454 G GGG GG G GGN Sbjct: 60 SGHGNGGGNG----NSGGGSGTGGNL 81 Score = 32.4 bits (73), Expect = 0.005 Identities = 27/89 (30%), Positives = 31/89 (34%) Query: 319 GDGGTGGNGGLLSGDGGAGGVGGTGGIQIYSGGGIGGVGGIGGNGGAGGTSGLIGDGGAG 378 G G G N G S G G G+ + G G GG G+ G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 379 GAGGTGGGNAGVGGVGGVGGTGGAARLFG 407 G GG G + G G GG A FG Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFG 91 Score = 32.4 bits (73), Expect = 0.006 Identities = 32/85 (37%), Positives = 35/85 (41%), Gaps = 8/85 (9%) Query: 335 GAGGVGGTGGIQIYSGGGIGGVGGIGGNGGAGGTSGLIGDGGAGGAGGTGGGNAGVGGVG 394 G G G G SG GG G+G GGA SG + G G G GG Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGS--GSGIHWGGGS 60 Query: 395 GVGGTGGAARLFGSGGSGGIGGTGG 419 G G GG +G SGG GTGG Sbjct: 61 GHGNGGG------NGNSGGGSGTGG 79 Score = 32.4 bits (73), Expect = 0.006 Identities = 31/97 (31%), Positives = 36/97 (37%), Gaps = 6/97 (6%) Query: 502 GNDGSGGAGGTGASGAGGNGGGGGAAGMMGDGGAGGDGGDGSAAGGL--GGDGGNADWLG 559 G DG G G ++ NGG G +G GG DG S+ GG G W G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTG----LGVGGGASDGSGWSSENNPWGGGSGSGIHWGG 58 Query: 560 NGGGGTGGFGLPPATGGFGGRGGRLFGSPGAQGRRAL 596 G G GG G G +P A G AL Sbjct: 59 GSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPAL 95 Score = 30.5 bits (68), Expect = 0.021 Identities = 26/77 (33%), Positives = 31/77 (40%) Query: 294 GGNAGLWGNGGAGGDGGFGGTATVIGDGGTGGNGGLLSGDGGAGGVGGTGGIQIYSGGGI 353 GG+ G G G T +G GG +G S + G G GI G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 354 GGVGGIGGNGGAGGTSG 370 G GG G +GG GT G Sbjct: 63 GNGGGNGNSGGGSGTGG 79
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_3508	IGASERPTASE	33	0.005	IgA-specific serine endopeptidase (S6) signature.
>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature. Length = 1541 Score = 32.7 bits (74), Expect = 0.005 Identities = 18/91 (19%), Positives = 39/91 (42%), Gaps = 1/91 (1%) Query: 2 SRGESRQARPSQSSRSRRVSGKAHSAHEPRQPRSSGKTQADRSPKQVREPKRLPQAKQAK 61 S E+++ + +++ + V + + E + + K + SPKQ + PQA+ A+ Sbjct: 1088 SGSETKETQTTETKETATVEKEEKAKVETEKTQEVPKVTSQVSPKQEQSETVQPQAEPAR 1147 Query: 62 KTRPAARTEVAPPGRSARERRTRQAVEVASR 92 + P + P ++ T Q + S Sbjct: 1148 ENDPTVNIK-EPQSQTNTTADTEQPAKETSS 1177
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_3509	PERTACTIN	31	0.013	Pertactin signature.
>PERTACTIN#Pertactin signature. Length = 922 Score = 30.8 bits (69), Expect = 0.013 Identities = 20/69 (28%), Positives = 27/69 (39%), Gaps = 2/69 (2%) Query: 201 LVQAPDGNWVVVGTPKPADGVPPPPLNSKLPEEGPPAPPKPAALPPEVPVRVMPGPDDPA 260 L +G W +VG P P P + + P P P PP+ P P+ PA Sbjct: 552 LAANGNGQWSLVGAKAPPAPKPAPQPGPQPGPQPPQPPQPPQ--PPQPPQPPQRQPEAPA 609 Query: 261 LLPRTGPQL 269 P G +L Sbjct: 610 PQPPAGREL 618

MUL_3667

MUL_3678

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
MUL_3667	0	16	3.488197	putative regulatory protein
MUL_3668	2	15	4.825151	anti sigma factor antagonist
MUL_3669	1	13	4.009987	hypothetical protein
MUL_3670	0	11	3.260949	transposase for IS2606
MUL_3671	1	13	3.111772	transposase for IS2606
MUL_3672	0	13	2.205434	glycyl-tRNA synthetase
MUL_3673	0	10	-0.939142	ArsR family transcriptional regulator
MUL_3674	0	10	0.374774	ferric uptake regulation protein FurB
MUL_3675	1	9	1.257531	hypothetical protein
MUL_3676	2	7	1.217868	undecaprenyl pyrophosphate synthase
MUL_3677	0	7	1.136731	DNA repair protein RecO
MUL_3678	0	9	0.302590	amidase

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_3667	TCRTETOQM	89	1e-20	Tetracycline resistance protein TetO/TetQ/TetM family ...
>TCRTETOQM#Tetracycline resistance protein TetO/TetQ/TetM family signature. Length = 639 Score = 89.1 bits (221), Expect = 1e-20 Identities = 45/119 (37%), Positives = 67/119 (56%), Gaps = 9/119 (7%) Query: 6 GVVDERSMRAQYLDRMDIERERGITIKAQNVRLPWQLDGTEYVLHLIDTPGHVDFTYEVS 65 G VD+ + R D +ER+RGITI+ W + T+ +++IDTPGH+DF EV Sbjct: 34 GSVDKGTTRT---DNTLLERQRGITIQTGITSFQW--ENTK--VNIIDTPGHMDFLAEVY 86 Query: 66 RALEACEGAVLLVDAAQGIEAQTLANLYLALDR-DLHIIPVLNKIDLPAADPDRYAGEI 123 R+L +GA+LL+ A G++AQT L+ AL + + I +NKID D +I Sbjct: 87 RSLSVLDGAILLISAKDGVQAQTRI-LFHALRKMGIPTIFFINKIDQNGIDLSTVYQDI 144 Score = 82.2 bits (203), Expect = 2e-18 Identities = 43/234 (18%), Positives = 84/234 (35%), Gaps = 18/234 (7%) Query: 120 AGEIAHIIGCEPGDVLRVSGKTGEGVADLLDHVVREVPPPQGDADAPTRAMIFDSVYDIY 179 E C V S K G+ +L++ + + + +F Y Sbjct: 202 QEESIRFHNCSLFPVYHGSAKNNIGIDNLIEVITNKFYSSTHRGQSELCGKVFKIEYSEK 261 Query: 180 RGVVTYVRVVDGKITPRERIAMMSTGATHELLEVGIVSPEPKASDGLGVGEVGYL---IT 236 R + Y+R+ G + R+ + + ++ E D GE+ L Sbjct: 262 RQRLAYIRLYSGVLHLRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFL 321 Query: 237 GVKDVWQSKVGDTVTTARKGATEALTGYREPKPMVYSGLYPVDGSDYPNLRDALDKLQLN 296 + V +GDT ++ E P P++ + + P L DAL ++ + Sbjct: 322 KLNSV----LGDTKLLPQRERIEN------PLPLLQTTVEPSKPQQREMLLDALLEISDS 371 Query: 297 DAALTYE-PETSVALGFGFRCGFLGLLHMEISRERLEREFDLDLISTSPNVVYR 349 D L Y + + FLG + ME++ L+ ++ +++ P V+Y Sbjct: 372 DPLLRYYVDSATHEIIL----SFLGKVQMEVTCALLQEKYHVEIEIKEPTVIYM 421 Score = 32.5 bits (74), Expect = 0.004 Identities = 16/81 (19%), Positives = 26/81 (32%), Gaps = 2/81 (2%) Query: 374 VYEPVVKTTIIAPSEFIGTIMELCQSRRGELGGMDYLSPERVELRYTMPLGEIIFDFFDS 433 + EP + I AP E++ + + E V L +P I ++ Sbjct: 535 LLEPYLSFKIYAPQEYLSRAYTDAPKYCANIVDTQLKNNE-VILSGEIPARC-IQEYRSD 592 Query: 434 LISRTRGYASLDYEESGEQEA 454 L T G + E G Sbjct: 593 LTFFTNGRSVCLTELKGYHVT 613
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_3668	PHPHLIPASEA1	28	0.028	Bacterial phospholipase A1 protein signature.
>PHPHLIPASEA1#Bacterial phospholipase A1 protein signature. Length = 289 Score = 27.6 bits (61), Expect = 0.028 Identities = 22/96 (22%), Positives = 36/96 (37%), Gaps = 14/96 (14%) Query: 56 PTAARARKLTYSPDHDGRADPGEIVWTWVVYEDDPTQGQDRPVLVVGRERNVLLALMLSS 115 P A A++ T HD A G I+ + Q D P + + N L+ Sbjct: 15 PMAVYAQEATVKEVHDAPAVRGSIIANML-------QEHDNPFTLYPYDTNYLIYT---- 63 Query: 116 QEQYSADPDWVAIGTGDWDFEGQQGWVRLDRVLDVP 151 ++D + AI + DW ++ V+ L P Sbjct: 64 ---QTSDLNKEAIASYDWAENARKDEVKFQLSLAFP 96
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_3671	cloacin	39	4e-05	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 38.9 bits (90), Expect = 4e-05 Identities = 34/90 (37%), Positives = 39/90 (43%), Gaps = 5/90 (5%) Query: 290 GDGGNGGAGGGQG-ANGGLGGLVIGNGGNRGIGGAGDSGNPAGGQGGNGGGAYLIGNGGW 348 G G N GA G NGG GL +G G + G G NP GG G+G G G Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGGASDG-SGWSSENNPWGGGSGSGIHW---GGGSG 61 Query: 349 GGVGGSGGDGGWLLGNGGNGAEGGTSSATG 378 G GG G+ G G GGN + A G Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAAPVAFG 91 Score = 34.7 bits (79), Expect = 6e-04 Identities = 31/105 (29%), Positives = 42/105 (40%), Gaps = 2/105 (1%) Query: 140 GNGGNGGDSTSAGVAGGAGGSAGLFGNGGAGGTGADADVSATNGGAGGAGGNAGLIFGFG 199 G G N G +++G G G GL GGA + + GG G+G + G G G Sbjct: 6 GRGHNTGAHSTSGNING--GPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHG 63 Query: 200 GAGGTGGSGINSFASFGGDGGAGGNSYLLGAAGAGGNGGVGLGTS 244 GG G SG S A ++ A G GG+ + S Sbjct: 64 NGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSIS 108 Score = 33.9 bits (77), Expect = 0.001 Identities = 27/92 (29%), Positives = 33/92 (35%), Gaps = 11/92 (11%) Query: 249 TGGDGGQGGLFGNGAAGGLGGQSTDGDGGSGGSGGRAGILYGDGGNGGAGGGQGANGGLG 308 +GGDG + +G + G T G G S G G GG Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDG-----------SGWSSENNPWGGGS 50 Query: 309 GLVIGNGGNRGIGGAGDSGNPAGGQGGNGGGA 340 G I GG G G G +GN GG G G + Sbjct: 51 GSGIHWGGGSGHGNGGGNGNSGGGSGTGGNLS 82 Score = 32.4 bits (73), Expect = 0.004 Identities = 28/90 (31%), Positives = 38/90 (42%), Gaps = 6/90 (6%) Query: 332 GQGGNGGGAYLIGN--GGWGGVGGSGGDGGWLLGNGGNGAEGGTSSATGGDGGNGGDARF 389 G+G N G GN GG G+G GG + N GG S + GG G Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH--- 62 Query: 390 IGNGGDGAHGGDGTPDGASGTGGSGGILFG 419 GNGG + G G+ G + + + + FG Sbjct: 63 -GNGGGNGNSGGGSGTGGNLSAVAAPVAFG 91 Score = 32.0 bits (72), Expect = 0.005 Identities = 37/129 (28%), Positives = 43/129 (33%), Gaps = 17/129 (13%) Query: 290 GDGGNGGAGGGQGANGGLGGLVIGNGGNRGIGGAGDSGNPAGGQGGNGGGAYLIGNGGWG 349 G G G G +G + NGG G+G G G + G + N WG Sbjct: 3 GGDGRGHNTGAHSTSGNI------NGGPTGLGVGG---------GASDGSGWSSENNPWG 47 Query: 350 GVGGSGGD--GGWLLGNGGNGAEGGTSSATGGDGGNGGDARFIGNGGDGAHGGDGTPDGA 407 G GSG GG GNGG G S TGG+ G G G Sbjct: 48 GGSGSGIHWGGGSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSI 107 Query: 408 SGTGGSGGI 416 S S I Sbjct: 108 SAGALSAAI 116 Score = 30.8 bits (69), Expect = 0.012 Identities = 25/79 (31%), Positives = 29/79 (36%) Query: 229 GAAGAGGNGGVGLGTSRFGGTGGDGGQGGLFGNGAAGGLGGQSTDGDGGSGGSGGRAGIL 288 G G G N G + G G GG +G+ G GSG G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 289 YGDGGNGGAGGGQGANGGL 307 GGNG +GGG G G L Sbjct: 63 GNGGGNGNSGGGSGTGGNL 81 Score = 30.5 bits (68), Expect = 0.015 Identities = 24/92 (26%), Positives = 34/92 (36%), Gaps = 1/92 (1%) Query: 119 GADGQTVNGVGQAGGDGGFLWGNGGNGGDSTSAGVAGGAGGSAGLFGNGGAGGTGADADV 178 GA + N G G G + G+G S + GG+G G G G G + + Sbjct: 12 GAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNGNS 71 Query: 179 SATNGGAGGAGGNAG-LIFGFGGAGGTGGSGI 209 +G G A + FGF G G+ Sbjct: 72 GGGSGTGGNLSAVAAPVAFGFPALSTPGAGGL 103 Score = 30.5 bits (68), Expect = 0.016 Identities = 39/117 (33%), Positives = 47/117 (40%), Gaps = 14/117 (11%) Query: 165 GNGGAGGTGADADVSATNGGAGGAGGNAGLIFGFGGAGGTGGSGINSFASFGGDGGAGGN 224 G+G TGA + NGG G G G G + GSG +S + G GG+G Sbjct: 4 GDGRGHNTGAHSTSGNINGGPTGLGV---------GGGASDGSGWSSENNPWG-GGSGSG 53 Query: 225 SYLLGAAGAGGNGGVGLGTSRFGGTGGDGGQGGLFGNGAAGGLGGQSTDGDGGSGGS 281 + G +G G GG G GG G GG A G ST G GG S Sbjct: 54 IHWGGGSGHGNGGGNGNS----GGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVS 106
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_3672	cloacin	38	8e-05	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 37.8 bits (87), Expect = 8e-05 Identities = 30/84 (35%), Positives = 34/84 (40%) Query: 155 GGGGGYAGLIGNGGAGGTGGNGGSGGLGGNAWLLGSGGTGGTGGAGVSSGFGGTGGNGGL 214 G G GN G TG G G G+ W + GG G+G+ G G GNGG Sbjct: 8 GHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGG 67 Query: 215 GGLLYGSGGAGGTGGAGAAGAVLG 238 G G G GG A AA G Sbjct: 68 NGNSGGGSGTGGNLSAVAAPVAFG 91 Score = 37.4 bits (86), Expect = 1e-04 Identities = 36/111 (32%), Positives = 43/111 (38%), Gaps = 2/111 (1%) Query: 265 GLTGQGGQGGDAGAGGASGYGIGNGGAGGAAGDGGGAGSLGTGGNGGSGGRAALLYGVGR 324 G G+G G G G G GG A DG G S GGSG + + +G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSG--SGIHWGGGS 60 Query: 325 DGGAGGAGGDGVGSAGGSGGLGGAAGLVDVGGDGGAGGAGGGAGGDGGAGA 375 G GG G+ G +G G L A V G + GG AGA Sbjct: 61 GHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGA 111 Score = 35.1 bits (80), Expect = 5e-04 Identities = 36/103 (34%), Positives = 43/103 (41%), Gaps = 1/103 (0%) Query: 244 GGDARLFGTGGAGGTGGDNSLGLTGQGGQGGDAGAGGASGYGIGNGGAGGAAGDGGGAGS 303 GGD R TG +G N G G G+G +S GG+G GGG+G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 304 LGTGGNGGSGGRAALLYGVGRDGGAGGAGGDGVGSAGGSGGLG 346 GGNG SGG + G A A G S G+GGL Sbjct: 63 GNGGGNGNSGGGSG-TGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 35.1 bits (80), Expect = 5e-04 Identities = 39/122 (31%), Positives = 45/122 (36%), Gaps = 7/122 (5%) Query: 118 NGADGATVGRI-GTPGGAGGLLYGNGGRGGDSTLAGVSGGGGGYAGLIGNGGAGGTGGNG 176 N +T G I G P G G + G G S GG G G G G GGNG Sbjct: 10 NTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNG 69 Query: 177 GSGGLGGNAWLLGSGGTGGTGGAGVSSGFGGTGGNGGLGGLLYGSGGAGGTGGAGAAGAV 236 SGG G+GG A V+ GF G G + S GA A A+ Sbjct: 70 NSGGGS------GTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAAIADIMAAL 123 Query: 237 LG 238 G Sbjct: 124 KG 125 Score = 34.3 bits (78), Expect = 0.001 Identities = 25/76 (32%), Positives = 33/76 (43%) Query: 321 GVGRDGGAGGAGGDGVGSAGGSGGLGGAAGLVDVGGDGGAGGAGGGAGGDGGAGAYLIGD 380 G G + GA G+ G G G GGA+ + G G G+G G G+ Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNG 65 Query: 381 GGDGGAGGHGGDGGAG 396 GG+G +GG G GG Sbjct: 66 GGNGNSGGGSGTGGNL 81 Score = 33.9 bits (77), Expect = 0.001 Identities = 36/124 (29%), Positives = 42/124 (33%), Gaps = 17/124 (13%) Query: 220 GSGGAGGTGGAGAAGAVLGGMGGLGGDARLFGTGGAGGTGGDNSLGLTGQGGQGGDAGAG 279 G G TG +G + GG GLG G G + G Sbjct: 4 GDGRGHNTGAHSTSGNINGGPTGLGV----------------GGGASDGSGWSSENNPWG 47 Query: 280 GASGYGIGNGGAGGAAGDGGGAGSLGTGGNGGSGGRAALLYGVGRDG-GAGGAGGDGVGS 338 G SG GI GG G GG S G G GG+ A G GAGG V Sbjct: 48 GGSGSGIHWGGGSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSI 107 Query: 339 AGGS 342 + G+ Sbjct: 108 SAGA 111 Score = 32.0 bits (72), Expect = 0.005 Identities = 28/82 (34%), Positives = 35/82 (42%), Gaps = 1/82 (1%) Query: 365 GGAGGDGGAGAYLIGDGGDGGAGGHGGDGGAG-GDGNAGSLAGGTGGNGGDAKVIGNGGN 423 GG G GA+ +GG G G GGA G G + GG+G G G+ Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 424 GGDGGVRFGGGANGSGGTGGAA 445 G GG GG +G+GG A Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAV 84 Score = 29.3 bits (65), Expect = 0.033 Identities = 25/80 (31%), Positives = 29/80 (36%), Gaps = 1/80 (1%) Query: 133 GAGGLLYGNGGRGGDSTLAGVSGGGGGYAGLIGNGGAGGTGGNGGSGGLGGNAWLLGSGG 192 G G + G + G G G G +G + N GG G G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGG-ASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 193 TGGTGGAGVSSGFGGTGGNG 212 G GG G S G GTGGN Sbjct: 62 HGNGGGNGNSGGGSGTGGNL 81
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_3675	56KDTSANTIGN	32	0.007	Rickettsia 56kDa type-specific antigen protein sign...
>56KDTSANTIGN#Rickettsia 56kDa type-specific antigen protein signature. Length = 533 Score = 31.9 bits (72), Expect = 0.007 Identities = 12/21 (57%), Positives = 13/21 (61%) Query: 533 NQQQPQQNQPQEGQHQQQQQQ 553 N P Q Q Q+GQ QQQQ Q Sbjct: 333 NFVMPPQAQQQQGQGQQQQAQ 353
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_3678	IGASERPTASE	30	0.018	IgA-specific serine endopeptidase (S6) signature.
>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature. Length = 1541 Score = 30.0 bits (67), Expect = 0.018 Identities = 17/51 (33%), Positives = 20/51 (39%), Gaps = 3/51 (5%) Query: 283 VTFDEDRHRAHTGNGAQVLATLRNTAINLHRLNGADNIAEACRITALTANR 333 V E+ H TGN L N I+ LN ADN + LT N Sbjct: 846 VRLTENSHWHLTGNSDVHQLDLANGHIH---LNSADNSNNVTKYNTLTVNS 893

MUL_3704

MUL_3712

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
MUL_3704	-1	12	1.679918	bifunctional enzyme MbtA: salicyl-AMP ligase
MUL_3705	-1	10	-0.299687	acetyl hydrolase MbtJ
MUL_3706	0	11	-0.305521	short-chain membrane-associated dehydrogenase
MUL_3708	3	11	0.572540	putative transcriptional regulator
MUL_3711	4	11	0.759920	acyl-[acyl-carrier protein] desaturase DesA1
MUL_3712	4	13	0.704986	hypothetical protein

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_3704	cloacin	39	4e-05	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 38.5 bits (89), Expect = 4e-05 Identities = 26/72 (36%), Positives = 32/72 (44%) Query: 327 GHGGGAGGAGGDAGDGGKGGDALDGGTAGTGGAGGNAGNGGGAGSGGKGTFDGGAGGAGG 386 GH GA G+ G G G + G+G + N GGG+GSG G G GG Sbjct: 8 GHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGG 67 Query: 387 DGGKAGNGGAGG 398 +G G G GG Sbjct: 68 NGNSGGGSGTGG 79 Score = 34.7 bits (79), Expect = 8e-04 Identities = 25/84 (29%), Positives = 32/84 (38%) Query: 252 AGRGGAADAGGPLVTPGHAGNTGTGGTGGTGGTGGNGDLHTDGGNGGTGGSGGAGIAGVG 311 +G G G T G+ TG G G + G+G + GG GSG G G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 312 GGNGGHGTDGSAGVAGHGGGAGGA 335 GNGG + G G + A Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVA 85 Score = 34.7 bits (79), Expect = 8e-04 Identities = 36/107 (33%), Positives = 44/107 (41%), Gaps = 4/107 (3%) Query: 220 TGGAGGTGGAGGSGVGSGLAGGTGGDGGVGGAAGRGGAADAGGPLVTPGHAGNTGTGGTG 279 +GG G G + GG G G GGA+ G + P G GG Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPW-GGGSGSGIHWGGGS 60 Query: 280 GTGGTGGNGDLHTDGGNGGTGGSGGAGIAGVGGGNGGHGTDGSAGVA 326 G G GGNG+ GG GTGG+ A A V G T G+ G+A Sbjct: 61 GHGNGGGNGN---SGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 32.4 bits (73), Expect = 0.004 Identities = 25/81 (30%), Positives = 33/81 (40%) Query: 296 NGGTGGSGGAGIAGVGGGNGGHGTDGSAGVAGHGGGAGGAGGDAGDGGKGGDALDGGTAG 355 +GG G G G G T G G + + GG G GG +G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 356 TGGAGGNAGNGGGAGSGGKGT 376 G GGN +GGG+G+GG + Sbjct: 62 HGNGGGNGNSGGGSGTGGNLS 82 Score = 32.4 bits (73), Expect = 0.004 Identities = 27/80 (33%), Positives = 35/80 (43%), Gaps = 1/80 (1%) Query: 358 GAGGNAGNGGGAGSGGKGTFDGGAGGAGGDGGKAGNGGAGGIDSNGQVADGGNGGDGGNG 417 G A + G +GG G G + G G + N GG S + GG G G G Sbjct: 8 GHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGG-SGSGIHWGGGSGHGNGG 66 Query: 418 GDGSPVGAGGTGGIGTAGGA 437 G+G+ G GTGG +A A Sbjct: 67 GNGNSGGGSGTGGNLSAVAA 86 Score = 31.6 bits (71), Expect = 0.006 Identities = 30/91 (32%), Positives = 40/91 (43%), Gaps = 10/91 (10%) Query: 284 TGGNGDLHTDGGNGGTGGSGGAGIAGVGGGNGGHGTDGSAGVAGHGGGAGGAGGDAGDGG 343 +GG+G H + G T G+ G G+G G G +DGS + + GG+G GG Sbjct: 2 SGGDGRGH-NTGAHSTSGNINGGPTGLGVGGG--ASDGSGWSSENNPWGGGSGSGIHWGG 58 Query: 344 KGGDALDGGTAGTGGAGGNAGNGGGAGSGGK 374 G G GG GN+G G G G Sbjct: 59 GSG-------HGNGGGNGNSGGGSGTGGNLS 82 Score = 30.5 bits (68), Expect = 0.015 Identities = 39/139 (28%), Positives = 52/139 (37%), Gaps = 7/139 (5%) Query: 195 GAGGTGGVGGANQFFGHAGDGGHGGTGGAGGTGGAGGSGVGSGLAGGTGGDGGVGGAAGR 254 G G G GA+ G+ G G G G + G+G S + GG+G GG +G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 255 GGAADAGGPLVTPGHAGNTGTGGTGGTGGTGGNGDLHTDGGNGG----TGGSGGAGIAGV 310 G G + G +G G G L T G G + G+ A IA + Sbjct: 63 GNGGGNGN---SGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAAIADI 119 Query: 311 GGGNGGHGTDGSAGVAGHG 329 G G GVA +G Sbjct: 120 MAALKGPFKFGLWGVALYG 138 Score = 30.5 bits (68), Expect = 0.016 Identities = 23/80 (28%), Positives = 31/80 (38%) Query: 164 AGSGGSGGAGGDGAPGDSGTAGGTGGTGSARGAGGTGGVGGANQFFGHAGDGGHGGTGGA 223 +G G G G + + G TG + G+G N + G +G G H G G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 224 GGTGGAGGSGVGSGLAGGTG 243 G GG G+ G GG Sbjct: 62 HGNGGGNGNSGGGSGTGGNL 81 Score = 29.7 bits (66), Expect = 0.026 Identities = 27/90 (30%), Positives = 32/90 (35%), Gaps = 9/90 (10%) Query: 152 GGAGGANGLFEAAGSGGSGGAGGDGAPGDSGTAGGTGGTGSARGAGGTGGVGGANQFFGH 211 GA +G +G G G G S GG GS G GG G Sbjct: 11 TGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGG-GSGSGIHWGGGSGH------- 62 Query: 212 AGDGGHGGTGGAGGTGGAGGSGVGSGLAGG 241 G+GG G G G G S V + +A G Sbjct: 63 -GNGGGNGNSGGGSGTGGNLSAVAAPVAFG 91
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_3708	HTHTETR	55	2e-11	TetR bacterial regulatory protein HTH signature.
>HTHTETR#TetR bacterial regulatory protein HTH signature. Length = 215 Score = 54.6 bits (131), Expect = 2e-11 Identities = 26/167 (15%), Positives = 57/167 (34%), Gaps = 11/167 (6%) Query: 6 RNAQANRRQRREQMECRLLEATERLMNNGASFTELSVDRLATEAGISRASFYIYFDDKGH 65 R + ++ R+ +L+ RL + + S+ +A AG++R + Y +F DK Sbjct: 3 RKTKQEAQETRQ----HILDVALRLFSQQ-GVSSTSLGEIAKAAGVTRGAIYWHFKDKSD 57 Query: 66 LLRRLAGQVFDDLATGAQHWWDVAWRHDPDDVRAAMCAII------ARYRRHQPILIALN 119 L + ++ + +R + ++ R R I+ Sbjct: 58 LFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVTEERRRLLMEIIFHKC 117 Query: 120 EMAGYEPQTAQTYRDILTAISARLARVIEDGQADGSIRPELSATTTA 166 E G Q R++ R+ + ++ + +L A Sbjct: 118 EFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMTRRAA 164
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_3711	CARBMTKINASE	37	9e-05	Bacterial carbamate kinase signature.
>CARBMTKINASE#Bacterial carbamate kinase signature. Length = 314 Score = 36.7 bits (85), Expect = 9e-05 Identities = 24/104 (23%), Positives = 43/104 (41%), Gaps = 7/104 (6%) Query: 156 DNDRLSALVAHLVGADALVLLSDIDGLYDADPRKFQNARFIPEVSGPADLDGVVAGQGSH 215 D D +A V AD ++L+D++G + +++ EV +L + H Sbjct: 214 DKDLAGEKLAEEVNADIFMILTDVNGAA-LYYGT-EKEQWLREVK-VEELRKYY--EEGH 268 Query: 216 LGTGGMASKMSSALLAADA-GVPVLLAPAADAAAALTDASVGTV 258 G M K+ +A+ + G ++A A AL + GT Sbjct: 269 FKAGSMGPKVLAAIRFIEWGGERAIIAHLEKAVEAL-EGKTGTQ 311
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_3712	SECA	32	0.007	SecA protein signature.
>SECA#SecA protein signature. Length = 901 Score = 31.8 bits (72), Expect = 0.007 Identities = 21/101 (20%), Positives = 40/101 (39%), Gaps = 29/101 (28%) Query: 387 IGQTNFDNDEAVGYLADRLVRLGVEEELL---------RLGAKPGC--AVTIGEMTFDWE 435 +G + + E +++ L + G++ +L + A+ G AVTI Sbjct: 454 VGTISIEKSE---LVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIA------- 503 Query: 436 PQTPAGGHVAMSGRGTDVRLERSDRVGAAERKAARRQRRER 476 M+GRGTD+ L S + A + ++ E+ Sbjct: 504 --------TNMAGRGTDIVLGGSWQAEVAALENPTAEQIEK 536

MUL_3782

MUL_3797

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
MUL_3782	-1	9	-0.582711	non-IS element not present in Mycobacterium
MUL_3784	-2	10	-0.668215	NAD synthetase
MUL_3785	-1	11	0.033646	Sir2-like regulatory protein
MUL_3786	-1	14	-0.412970	AcrR family transcriptional regulator
MUL_3787	-1	13	-0.781352	gamma-glutamyl kinase
MUL_3788	-1	13	-0.622645	GTPase ObgE
MUL_3790	0	11	-1.482529	50S ribosomal protein L27
MUL_3792	-1	10	-1.468556	50S ribosomal protein L21
MUL_3793	-2	9	-1.067881	ribonuclease E Rne
MUL_3794	-2	8	-0.763438	non-IS element not present in Mycobacterium
MUL_3796	-2	8	-1.219592	nucleoside diphosphate kinase
MUL_3797	-1	10	-1.454270	hypothetical protein

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_3782	TYPE3OMGPROT	29	0.017	Type III secretion system outer membrane G protein ...
>TYPE3OMGPROT#Type III secretion system outer membrane G protein family signature. Length = 607 Score = 29.1 bits (65), Expect = 0.017 Identities = 20/69 (28%), Positives = 25/69 (36%), Gaps = 14/69 (20%) Query: 124 GTQIADGGLPWRYDASGAVAVVSPPKETREF--DGATYVLE-----RGIRTD------FA 170 + I + WR DAS + VS P E A LE R +T F Sbjct: 130 RSGIWEPRFGWRPDASNRLVYVSGPPRYLELVEQTAA-ALEQQTQIRSEKTGALAIEIFP 188 Query: 171 LVHAWKGDR 179 L +A DR Sbjct: 189 LKYASASDR 197
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_3785	HTHTETR	74	2e-18	TetR bacterial regulatory protein HTH signature.
>HTHTETR#TetR bacterial regulatory protein HTH signature. Length = 215 Score = 74.3 bits (182), Expect = 2e-18 Identities = 37/183 (20%), Positives = 65/183 (35%), Gaps = 9/183 (4%) Query: 13 PNRRSQQKSDRRLQLLSAAERLFAERGFLAVRLEDIGAAAGISGPAIYRHFPNKESLLVE 72 + Q+ + R +L A RLF+++G + L +I AAG++ AIY HF +K L E Sbjct: 2 ARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSE 61 Query: 73 LLVGISSRLLAGARQVTTN-SNDAAAALDGLIDFHLDFALGEPDLIRIQDRDLGHLPAAA 131 + S + + D + L ++ L+ + E + + Sbjct: 62 IWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVTEERRRLLMEIIFHKCEFVG 121 Query: 132 ERQ-VRKAQRQYVEIWVGVLRQL------DPGLAEA-DARLMAHAAFGLLNSTPHSMESA 183 E V++AQR + Q L R A G ++ + A Sbjct: 122 EMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMTRRAAIIMRGYISGLMENWLFA 181 Query: 184 DTK 186 Sbjct: 182 PQS 184
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_3786	SURFACELAYER	30	0.013	Lactobacillus surface layer protein signature.
>SURFACELAYER#Lactobacillus surface layer protein signature. Length = 439 Score = 29.6 bits (66), Expect = 0.013 Identities = 19/119 (15%), Positives = 38/119 (31%), Gaps = 5/119 (4%) Query: 128 ALEPLPPIAGPSSTIAPPTTRTSPTPSSSPAPTTTSGSATPTTTPTAGAMQTVVYTVTGE 187 AL + PIA + + TT + + ++ TP+ + A ++ Sbjct: 14 ALLAVAPIAATAMPVNAATTINADSAINANTNAKYDVDVTPSISAIAAVAKSDTMPAIPG 73 Query: 188 GRAISVTYMDTGDVIQTEFNVALPWNREVSLSRSANHPASVTIVNIGHNVTCSVTVSGV 246 S++ G + +++ S N+ + T VTV V Sbjct: 74 SLTGSISASYNGKSYTANLP---KDSGNATITDSNNNTVKPAELEADKAYT--VTVPDV 127
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_3787	TCRTETA	31	0.008	Tetracycline resistance protein signature.
>TCRTETA#Tetracycline resistance protein signature. Length = 399 Score = 31.3 bits (71), Expect = 0.008 Identities = 77/416 (18%), Positives = 141/416 (33%), Gaps = 48/416 (11%) Query: 24 STGLNAMVTTFVFSVYLTSMVGEGMPGGSDPASWLGRAAAAAGLTIALLAPLVGVWVESP 83 + L+A+ + V L ++ + + D + G A L AP++G + Sbjct: 13 TVALDAVGIGLIMPV-LPGLLRDLVHSN-DVTAHYGILLALYALMQFACAPVLGALSDRF 70 Query: 84 HRRRVALGVLTSVTVALTCAMFLIHDSPGYLWAGLALLAATAACSDLASVPYNSMLRQLS 143 RR V L L V A+ L+ G + T A +A + + ++ Sbjct: 71 GRRPVLLVSLAGAAVDY--AIMATAPFLWVLYIGRIVAGITGATGAVAG----AYIADIT 124 Query: 144 TPTTASRISGFGWASGYVGSVVLLILIYLGFVAGSGAHRGLLQLPAHDGFNIRVAMLLAA 203 R FG+ S G G VAG + H F AA Sbjct: 125 --DGDERARHFGFMSACFGF---------GMVAGPVLGGLMGGFSPHAPF-------FAA 166 Query: 204 AWLAVLASPLLLVAHRLPDVGTVPQPATRMLGAYRKLWADISSEWRRDRNLVYFLIASAI 263 A L L L LP+ R L S W R +V L+A Sbjct: 167 AALNGLN--FLTGCFLLPE----SHKGERRPLRREALNPLASFRWARGMTVVAALMAVFF 220 Query: 264 FRDGLAAMFA-FGAVLGANVYGLTQADVLLFGVAASVVAAVGA----VVGGFVDHRVGSK 318 + + A + G + + + G++ + + + ++ G V R+G + Sbjct: 221 IMQLVGQVPAALWVIFGEDRFHWDATTI---GISLAAFGILHSLAQAMITGPVAARLGER 277 Query: 319 PVIVASLASILISAIVLMVLSGATAFWVCGLLLCLFI--GPSQSSARALLLRMAPHGKEG 376 + + ++ ++L+ AT W+ ++ L G + +A+L R ++G Sbjct: 278 RAL---MLGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQG 334 Query: 377 VAFGLYTMTGHAVAFLGPWLFSIFVDIFSAVRAGLGGICLVLAVGLVGMLVVGVPR 432 G + +GP LF+ I++A G + L + + + R Sbjct: 335 QLQGSLAALTSLTSIVGPLLFTA---IYAASITTWNGWAWIAGAALYLLCLPALRR 387
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_3788	DHBDHDRGNASE	79	3e-19	2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase signature. Length = 261 Score = 78.6 bits (193), Expect = 3e-19 Identities = 57/185 (30%), Positives = 93/185 (50%), Gaps = 3/185 (1%) Query: 12 AVVTGASQNIGEALATELAARGHNLIVTARRESLLNELAARLTDKYRVSVEVRPADLADP 71 A +TGA+Q IGEA+A LA++G ++ L ++ + L + R + E PAD+ D Sbjct: 11 AFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHA-EAFPADVRDS 69 Query: 72 QERTKLTDELAAR--PISILCANAGTATFGPVASLDPAGEKAQVQLNAVAVHDLTLAVLP 129 ++T + PI IL AG G + SL +A +N+ V + + +V Sbjct: 70 AAIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGVFNASRSVSK 129 Query: 130 GMIERRAGGILISGSAAGNSPIPYNATYAATKAFANTFSESLRGELRGSGVHVTLLAPGP 189 M++RR+G I+ GS P A YA++KA A F++ L EL + +++PG Sbjct: 130 YMMDRRSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLELAEYNIRCNIVSPGS 189 Query: 190 VRTDL 194 TD+ Sbjct: 190 TETDM 194
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_3793	HTHTETR	54	6e-11	TetR bacterial regulatory protein HTH signature.
>HTHTETR#TetR bacterial regulatory protein HTH signature. Length = 215 Score = 53.9 bits (129), Expect = 6e-11 Identities = 25/179 (13%), Positives = 59/179 (32%), Gaps = 18/179 (10%) Query: 17 RDRRRAELFSLIQQTAHRLFAERGFDAVTTEDIAAAAGVSISTYFRHAPTKEGLLVDPVR 76 ++ R+ I A RLF+++G + + +IA AAGV+ + H K L + Sbjct: 10 QETRQH-----ILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSEIWE 64 Query: 77 QAITEIVSSYR---------SWPADESAVEASIALFVSYARDAGDLKLDTLRRAIATAPY 127 + + I + + V+ R +++ + Sbjct: 65 LSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVTEERRRLLMEIIFHKCEFVGEMA 124 Query: 128 LLSKSALVSEDDQHRFIEHVASRMGVDA---RTDIRPALLVHTSLATVKFVFDRWLSTD 183 ++ ++ + + IE + ++A D+ + + + WL Sbjct: 125 VVQQAQRNLCLESYDRIEQTL-KHCIEAKMLPADLMTRRAAIIMRGYISGLMENWLFAP 182
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_3796	PF05616	30	0.024	Neisseria meningitidis TspB protein
>PF05616#Neisseria meningitidis TspB protein Length = 501 Score = 30.1 bits (67), Expect = 0.024 Identities = 25/73 (34%), Positives = 30/73 (41%), Gaps = 9/73 (12%) Query: 383 PPKDLAPPPGTAVGPDGN-LVALGP---PLINPSPNL---TDPNPPLPAWLTPSPRVPGT 435 P DL P G+A P+ L + P P NP+PN T PNP L P Sbjct: 311 PRPDLTP--GSAEAPNAQPLPEVSPAENPANNPAPNENPGTRPNPEPDPDLNPDANPDTD 368 Query: 436 GDPDDAPPAPPAP 448 G P P +P P Sbjct: 369 GQPGTRPDSPAVP 381
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_3797	PF07675	29	0.040	Cleaved Adhesin
>PF07675#Cleaved Adhesin Length = 1358 Score = 28.9 bits (64), Expect = 0.040 Identities = 23/57 (40%), Positives = 28/57 (49%), Gaps = 4/57 (7%) Query: 115 SPDQLAPGSVIPVQRTEPSFDVTALLNGYEPLFSLLNPRDADNL--TKGIIESLQGD 169 D GSVIP T P F TA N Y F L P +AD + T+ II + QG+ Sbjct: 409 DADHNTFGSVIPA--TGPLFTGTASSNLYSANFEYLTPANADPVVTTQNIIVTGQGE 463

MUL_3843

MUL_3853

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
MUL_3843	0	14	-1.888111	hypothetical protein
MUL_3844	1	14	-1.843681	bifunctional transmembrane phospholipid
MUL_3846	0	13	-1.814567	hypothetical protein
MUL_3847	0	12	-1.945787	carboxylesterase LipQ
MUL_3848	1	13	-2.199092	*hypothetical protein
MUL_3849	1	11	-2.457241	cobyric acid synthase
MUL_3853	1	11	-2.018322	hypothetical protein

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_3843	BCTLIPOCALIN	32	0.002	Bacterial lipocalin signature.
>BCTLIPOCALIN#Bacterial lipocalin signature. Length = 171 Score = 31.9 bits (72), Expect = 0.002 Identities = 18/51 (35%), Positives = 25/51 (49%), Gaps = 4/51 (7%) Query: 139 LVPATLGAPEGTVAASDIDLHLVLGSW----RILGAVQQTLDIVTAHVRTR 185 L+ LG PE SD +L+ LG W R+ + ++ L VTA R R Sbjct: 12 LLNGCLGMPESVKPVSDFELNNYLGKWYEVARLDHSFERGLSQVTAEYRVR 62
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_3848	HTHTETR	75	4e-18	TetR bacterial regulatory protein HTH signature.
>HTHTETR#TetR bacterial regulatory protein HTH signature. Length = 215 Score = 75.4 bits (185), Expect = 4e-18 Identities = 28/214 (13%), Positives = 70/214 (32%), Gaps = 5/214 (2%) Query: 1 MAGPSEQVEATRADRFIKTAVEILGETGRTDFTVQEVVTRSKTSLRAFYQHFSSKDELLL 60 MA ++Q + A+ + + G + ++ E+ + + A Y HF K +L Sbjct: 1 MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFS 60 Query: 61 ALFDRTMSQTAQLWR--AEAAGLDSTAALKLVIDRISAQPESSTQDSLNRALSLYNQHLA 118 +++ + S +L D + L+ ++ + + + L + + Sbjct: 61 EIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVTEERRRLLMEIIFHKCEFV 120 Query: 119 ETRP---REYARVLSPLHRLIRDIVGQGITEGMFNPGLDVGAAAAIVMQTVLGALRLRWL 175 + + + I + I M L AA I+ + G + Sbjct: 121 GEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMTRRAAIIMRGYISGLMENWLF 180 Query: 176 GTELNAMPIDAGELYEFCSRALGVRDTEESAASS 209 + + +A + + T + A++ Sbjct: 181 APQSFDLKKEARDYVAILLEMYLLCPTLRNPATN 214
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_3849	PF05775	29	0.011	Enterobacteria AfaD invasin protein
>PF05775#Enterobacteria AfaD invasin protein Length = 142 Score = 29.5 bits (66), Expect = 0.011 Identities = 13/56 (23%), Positives = 23/56 (41%), Gaps = 3/56 (5%) Query: 252 TAFRMLAMSKRPIEDAMGALVCHGALSRNPELRILSIKNGADWVPTLFKGLKGVYK 307 + FR+ ++ R G + LRI +G W + KG++GV+ Sbjct: 56 SGFRVW-INARQEGGGAGKYIVQSTEGPQHNLRIRI--SGNGWSSFVEKGIQGVFN 108
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_3853	PF06580	36	4e-04	Sensor histidine kinase
>PF06580#Sensor histidine kinase Length = 349 Score = 36.0 bits (83), Expect = 4e-04 Identities = 13/55 (23%), Positives = 22/55 (40%), Gaps = 1/55 (1%) Query: 468 FVENAVEHGYSTDVSDGLAVAAALGGDGKLRVAVIDHGTWKHHREGERGRGRGLA 522 VEN ++HG + G + +G + + V + G+ E G GL Sbjct: 263 LVENGIKHGIAQLPQGGKILLKGTKDNGTVTLEVENTGSLALKNTKE-STGTGLQ 316

MUL_3918

MUL_3921

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
MUL_3918	0	10	-0.866123	transposase for IS2404
MUL_3919	0	10	-1.075535	transposase for IS2404
MUL_3920	-1	11	-0.208660	coenzyme F420-dependent oxidoreductase
MUL_3921	0	11	-0.363756	acyl-CoA dehydrogenase

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_3918	PERTACTIN	33	0.003	Pertactin signature.
>PERTACTIN#Pertactin signature. Length = 922 Score = 32.8 bits (74), Expect = 0.003 Identities = 22/62 (35%), Positives = 26/62 (41%), Gaps = 6/62 (9%) Query: 429 APDGTPLW----PGLPPAPPPGAPRESGPTPGSEPFVVPASAQAQPTPLPPAPLPQEVAP 484 A +G W PPAP P + GP PG +P P Q P PP P+ AP Sbjct: 553 AANGNGQWSLVGAKAPPAPKPAP--QPGPQPGPQPPQPPQPPQPPQPPQPPQRQPEAPAP 610 Query: 485 SP 486 P Sbjct: 611 QP 612
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_3919	BACINVASINC	28	0.049	Salmonella/Shigella invasin protein C signature.
>BACINVASINC#Salmonella/Shigella invasin protein C signature. Length = 409 Score = 28.3 bits (62), Expect = 0.049 Identities = 23/136 (16%), Positives = 55/136 (40%), Gaps = 8/136 (5%) Query: 117 GSPKQLKPGDTIPMTHTSPALDLDALIGGFRPLLKALDPDQVNALSGQLIRALQGEGATI 176 G+ D ++ + ++L G + K + P+ LS +L +++ + Sbjct: 252 GTDATKNLNDATLKSNAGTSA-TESL--GIKNSNKQISPEHQAILSKRLE-SVESDIRLE 307 Query: 177 NSFLAQTAALTTTLADRDQLIGDVIINLNVVLGSLGDQNKQFAKAVDALAELMEGLQARK 236 T +T A + Q+ GD+I+ +V +G + ++Q+A + + + + R Sbjct: 308 Q----NTMDMTRIDARKMQMTGDLIMKNSVTVGGIAGASRQYAATQERSEQQISQVNNRV 363 Query: 237 EDITKGMAYTNAAASS 252 A ++ S+ Sbjct: 364 ASTASDEARESSRKST 379
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_3920	PRTACTNFAMLY	32	0.004	Pertactin virulence factor family signature.
>PRTACTNFAMLY#Pertactin virulence factor family signature. Length = 910 Score = 32.3 bits (73), Expect = 0.004 Identities = 15/49 (30%), Positives = 15/49 (30%) Query: 388 PLPAPPPGGPPPGPPAPAPPELASIPQPTPSSVLVPAPGEVSAPQTAGA 436 P P P P P P P P A PQP L A G Sbjct: 573 PAPKPAPQPGPQPPQPPQPQPEAPAPQPPAGRELSAAANAAVNTGGVGL 621
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_3921	PF03544	30	0.024	Gram-negative bacterial tonB protein
>PF03544#Gram-negative bacterial tonB protein Length = 243 Score = 29.6 bits (66), Expect = 0.024 Identities = 6/41 (14%), Positives = 9/41 (21%) Query: 386 PDYIPPQAIPPQQAPPAAAPPAQAPPPAVGLPLPAEAPATP 426 P I P+ P Q + +P Sbjct: 91 PVVIEKPKPKPKPKPKPVKKVEQPKRDVKPVESRPASPFEN 131

MUL_4272

MUL_4280

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
MUL_4272	0	12	3.103988	PE family protein
MUL_4273	0	13	2.965435	transposase for IS2404
MUL_4274	0	13	3.057403	lipoprotein LpqG
MUL_4275	2	14	5.904588	hypoxanthine-guanine phosphoribosyltransferase
MUL_4276	2	14	5.238496	cell cycle protein MesJ
MUL_4277	1	13	5.084098	hypothetical protein
MUL_4278	0	14	4.805945	D-alanyl-D-alanine carboxypeptidase
MUL_4279	1	13	4.333477	inorganic pyrophosphatase Ppa
MUL_4280	0	12	4.382156	hypothetical protein

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_4272	BACINVASINB	38	5e-05	Salmonella/Shigella invasin protein B signature.
>BACINVASINB#Salmonella/Shigella invasin protein B signature. Length = 593 Score = 38.2 bits (88), Expect = 5e-05 Identities = 35/140 (25%), Positives = 65/140 (46%), Gaps = 21/140 (15%) Query: 236 LAGLVVVILVGVAAAANGATAALLGFPLVLLVGLLVAYLYTVLMFA-----PVL-IVLER 289 L L+ ++ V A GA+ AL L ++V + T + F P++ VL+ Sbjct: 321 LGALLTIVSVVAAVFTGGASLALAAVGLAVMVADEIVKAATGVSFIQQALNPIMEHVLK- 379 Query: 290 LPLVDAITRSFALVTGGFWRVLGIRLLTAIVVGLVGGAISAPFGIVGQILLGATASEGST 349 PL++ I ++ G LG+ TA + G + GAI A +V I++ A +G+ Sbjct: 380 -PLMELIGKAITKALEG----LGVDKKTAEMAGSIVGAIVAAIAMVAVIVVVAVVGKGAA 434 Query: 350 GMFLVGMTLSSIGSAISQII 369 + +G+A+S+++ Sbjct: 435 ---------AKLGNALSKMM 445
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_4275	HTHFIS	38	5e-05	FIS bacterial regulatory protein HTH signature.
>HTHFIS#FIS bacterial regulatory protein HTH signature. Length = 484 Score = 37.5 bits (87), Expect = 5e-05 Identities = 44/207 (21%), Positives = 67/207 (32%), Gaps = 22/207 (10%) Query: 117 DEINRTPPKTQAALLEAMEERQVSVEGQAKPLP-DPFIVAATQNPIEYEGTHQLPEAQLD 175 DEI P Q LL +++ + + G P+ D IVAAT ++ L L Sbjct: 238 DEIGDMPMDAQTRLLRVLQQGEYTTVGGRTPIRSDVRIVAATNKDLKQSINQGLFREDL- 296 Query: 176 RFLLKLNVG---LPS---RESEIAILGRH-----------AHGFDPRDLSAIKPVAGPAE 218 +LNV LP R +I L RH FD L +K P Sbjct: 297 --YYRLNVVPLRLPPLRDRAEDIPDLVRHFVQQAEKEGLDVKRFDQEALELMKAHPWPGN 354 Query: 219 LAAGREAVSRVLIADEVLGYIVDIVGATRSSPALQLGVSPRGATALLGTARSWAWLSGRN 278 + V R+ +I+ S + A + + + R Sbjct: 355 VRELENLVRRLTALYPQDVITREIIENELRSEIPDSPIEKAAARSGSLSISQAVEENMRQ 414 Query: 279 YVTPDDVKAMARPTLRHRIMLRLEAEL 305 Y A+ L R++ +E L Sbjct: 415 Y-FASFGDALPPSGLYDRVLAEMEYPL 440
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_4278	PERTACTIN	32	0.004	Pertactin signature.
>PERTACTIN#Pertactin signature. Length = 922 Score = 31.6 bits (71), Expect = 0.004 Identities = 18/45 (40%), Positives = 19/45 (42%) Query: 248 GAGAPPGWPPQTPPAPVWWPGQPAPQPLIQPPFAPDPAPSPPQGP 292 GA APP P P P P P P QPP P P P+ P Sbjct: 564 GAKAPPAPKPAPQPGPQPGPQPPQPPQPPQPPQPPQPPQRQPEAP 608
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_4279	cloacin	29	0.020	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 28.9 bits (64), Expect = 0.020 Identities = 15/37 (40%), Positives = 16/37 (43%) Query: 76 PLGFGGGFGPGFGPGLGFGFGPGGARGGGRRGGPGRG 112 P G G G G +G G G G G G GG G G Sbjct: 45 PWGGGSGSGIHWGGGSGHGNGGGNGNSGGGSGTGGNL 81
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_4280	cloacin	39	5e-05	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 39.3 bits (91), Expect = 5e-05 Identities = 43/112 (38%), Positives = 50/112 (44%), Gaps = 11/112 (9%) Query: 536 GDGGGGGGGASGGGGGASGGTGGTGGAGGLLSAGGAGGVGGAGGYNTSGPGGNGGSGGNA 595 GDG G GA G +GG G G GG G+G + + P G GGSG Sbjct: 4 GDGRGHNTGAHSTSGNINGGPTGLGVGGG--------ASDGSGWSSENNPWG-GGSGSGI 54 Query: 596 GTLFGSGGGGNGGSGYSGIGGTGGTGGNAVLLGGGGAGGAGGISFTGAGGQG 647 GSG G GG+G SG G GTGGN + A G +S GAGG Sbjct: 55 HWGGGSGHGNGGGNGNSG--GGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 38.9 bits (90), Expect = 8e-05 Identities = 46/130 (35%), Positives = 54/130 (41%), Gaps = 13/130 (10%) Query: 147 GAGGAGGAGGAGGSSTGGAGGTGGAGGAGEWLFGPGGVGGAGGSSSSAGGAGGVGGAGGL 206 G G GA G+ GG G G GGA + G SS GG G+G Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGGASD----------GSGWSSENNPWGGGSGSGIH 55 Query: 207 FGGGLGGAGGAGVSASGGAGGAGGAGGALAGFLGAGG---SDGGAGGTGVNHEGGAGGAG 263 +GGG G G G SGG G GG A+A + G S GAGG V+ GA A Sbjct: 56 WGGGSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAA 115 Query: 264 GAGGLIAGTG 273 A + A G Sbjct: 116 IADIMAALKG 125 Score = 38.2 bits (88), Expect = 1e-04 Identities = 37/116 (31%), Positives = 41/116 (35%), Gaps = 6/116 (5%) Query: 123 GNDGAGGSGAAGSAGGAGGAAGLIGAGGAGGAGGAGGSSTGGAGGTGGAGGAGEWLFGPG 182 G DG G + A S G G G + G+G SS G G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 183 GVGGAGGSSSSAGGAGGVGG------AGGLFGGGLGGAGGAGVSASGGAGGAGGAG 232 G GG G+S G GG A G GAGG VS S GA A A Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAAIAD 118 Score = 34.7 bits (79), Expect = 0.001 Identities = 31/100 (31%), Positives = 37/100 (37%), Gaps = 3/100 (3%) Query: 628 GGGGAGGAGGISFTGAGGQGGAGGTGGQLSGNGGSGGTGGEGDYVGGADSGGAGGAGGNA 687 GG G G G T GG G G + GSG + + GG+ SG G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 688 GLTGDGGNGGSGGTPGSPGGGGTGGALIGQDGLARLAVTG 727 G G GN G G GG + A G L+ G Sbjct: 63 GNGGGNGNSGGG---SGTGGNLSAVAAPVAFGFPALSTPG 99 Score = 33.9 bits (77), Expect = 0.002 Identities = 33/88 (37%), Positives = 41/88 (46%), Gaps = 8/88 (9%) Query: 570 GAGGVGGAGGYNTSGPGGNGGSGGNAGTLFGSGGGGNGGSGYSGIGGTGGTGGNAVLLGG 629 G G G G +++ NGG G G GGG + GSG+S G G + + G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTG-----LGVGGGASDGSGWSSENNPWGGGSGSGIHWG 57 Query: 630 GGAGGAGGISFTGAGGQGGAGGTGGQLS 657 GG+G G G G GG GTGG LS Sbjct: 58 GGSGHGNG---GGNGNSGGGSGTGGNLS 82 Score = 33.1 bits (75), Expect = 0.004 Identities = 32/101 (31%), Positives = 42/101 (41%), Gaps = 7/101 (6%) Query: 243 GSDGGAGGTGVNHEGGAGGAGGAGGLIAGTG------GNGGAGGTDAYSRGGAGGAGGTG 296 G + GA T N GG G G GG G+G GG G+ + GG+G G G Sbjct: 8 GHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGG 67 Query: 297 GTGGTDMSDSGGTGGAGGNA-GLLFGSGGAGGAGGAAVALN 336 S +GG A F + GAGG AV+++ Sbjct: 68 NGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSIS 108 Score = 33.1 bits (75), Expect = 0.004 Identities = 32/95 (33%), Positives = 38/95 (40%) Query: 497 GDGGAAGLLGTGGTGGAGARLAGGAGGTGGAGGAGGWLLGDGGGGGGGASGGGGGASGGT 556 G +G + G TG A G G G G GGG+ G GG +G + Sbjct: 12 GAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNGNS 71 Query: 557 GGTGGAGGLLSAGGAGGVGGAGGYNTSGPGGNGGS 591 GG G GG LSA A G +T G GG S Sbjct: 72 GGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVS 106 Score = 32.8 bits (74), Expect = 0.006 Identities = 29/82 (35%), Positives = 34/82 (41%), Gaps = 5/82 (6%) Query: 569 GGAGGVGGAGGYNTSGPGGNG-GSGGNAGTLFGSGGGGNGGSGYSGIGGTGGTGGNAVLL 627 G G G GP G G G G + G+ + S GG SGI GG+G Sbjct: 8 GHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHG---- 63 Query: 628 GGGGAGGAGGISFTGAGGQGGA 649 GGG G +GG S TG A Sbjct: 64 NGGGNGNSGGGSGTGGNLSAVA 85 Score = 32.4 bits (73), Expect = 0.006 Identities = 31/93 (33%), Positives = 41/93 (44%), Gaps = 9/93 (9%) Query: 248 AGGTGVNHEGGAGGA-----GGAGGLIAGTGGNGGAGGTDAYSRGGAGGAGGTGGTGGTD 302 +GG G H GA GG GL G G + G+G + + G G G GG+ Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 303 MSDSGGTGGAGGNAGLLFGSGGAGGAGGAAVAL 335 + GG G +GG + G+GG A A VA Sbjct: 62 HGNGGGNGNSGGGS----GTGGNLSAVAAPVAF 90 Score = 32.4 bits (73), Expect = 0.008 Identities = 24/79 (30%), Positives = 31/79 (39%) Query: 483 AGSGTDGTPGGWLLGDGGAAGLLGTGGTGGAGARLAGGAGGTGGAGGAGGWLLGDGGGGG 542 +G G G G G G GG + +G + GG G + GGG G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 543 GGASGGGGGASGGTGGTGG 561 G GG G + GG+G G Sbjct: 62 HGNGGGNGNSGGGSGTGGN 80 Score = 31.2 bits (70), Expect = 0.014 Identities = 28/84 (33%), Positives = 35/84 (41%), Gaps = 4/84 (4%) Query: 600 GSGGGGNGGSGYSGIGGTGGTGGNAVLLGGGGAGGAGGISFTGAGGQGGAGGTGGQLSGN 659 G G G N G+ + GG G LG GG G + GG G+G G Sbjct: 4 GDGRGHNTGAHSTSGNINGGPTG----LGVGGGASDGSGWSSENNPWGGGSGSGIHWGGG 59 Query: 660 GGSGGTGGEGDYVGGADSGGAGGA 683 G G GG G+ GG+ +GG A Sbjct: 60 SGHGNGGGNGNSGGGSGTGGNLSA 83 Score = 31.2 bits (70), Expect = 0.015 Identities = 37/109 (33%), Positives = 47/109 (43%), Gaps = 3/109 (2%) Query: 203 AGGLFGGGLGGAGGAGVSASGGAGGAGGAGGAL--AGFLGAGGSDGGAGGTGVNHEGGAG 260 +GG G GA + +GG G G GGA +G+ GG G+G++ GG+G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 261 -GAGGAGGLIAGTGGNGGAGGTDAYSRGGAGGAGGTGGTGGTDMSDSGG 308 G GG G G G GG A A T G GG +S S G Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAG 110 Score = 31.2 bits (70), Expect = 0.017 Identities = 35/115 (30%), Positives = 41/115 (35%), Gaps = 6/115 (5%) Query: 298 TGGTDMSDSGGTGGAGGNAGLLFGSGGAGGAGGAAVALNDVGGAGGAGGNAGLFGNGGVG 357 +GG + G GN G G G GGA +D G G G+ Sbjct: 2 SGGDGRGHNTGAHSTSGNIN--GGPTGLGVGGGA----SDGSGWSSENNPWGGGSGSGIH 55 Query: 358 GVGGVGAGDGGAGGRAGLVIGNGGAGGAGGESFGFGAGVGGAGGNGGNGVLIGNG 412 GG G G+GG G +G G GG A FG G GG V I G Sbjct: 56 WGGGSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAG 110 Score = 30.1 bits (67), Expect = 0.038 Identities = 28/78 (35%), Positives = 34/78 (43%), Gaps = 4/78 (5%) Query: 271 GTGGNGGAGGTDAYSRGGAGGAGGTGG-TGGTDMSDSGGTGGAGGNAGLLFGSGGAGGAG 329 G G N GA T GG G G GG + G+ S G G +G+ +G G G G Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNG 65 Query: 330 GAAVALNDVGGAGGAGGN 347 G + GG G GGN Sbjct: 66 GGN---GNSGGGSGTGGN 80 Score = 30.1 bits (67), Expect = 0.041 Identities = 33/98 (33%), Positives = 40/98 (40%), Gaps = 7/98 (7%) Query: 340 GAGGAGGNAGLFGNGGV--GGVGGVGAGDGGAGGRAGLVIGNGGAGGAGGESFGFGAGVG 397 G G G N G G GG G+G G G + G N GG+G G G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSG---SGIHWGGG 59 Query: 398 GAGGNGGNGVLIGNGGNAGTGGTGLSTGSTGAGGISGL 435 GNGG +GG +GTGG + + A G L Sbjct: 60 SGHGNGGGNG--NSGGGSGTGGNLSAVAAPVAFGFPAL 95

MUL_4381

MUL_4391

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
MUL_4381	2	13	3.973918	DeoR family transcriptional regulator
MUL_4383	-1	11	0.180498	hypothetical protein
MUL_4384	-1	10	-0.342069	DNA polymerase III subunit epsilon
MUL_4385	0	10	-0.794217	UDP-N-acetylmuramyl tripeptide synthase
MUL_4386	0	10	-1.526386	cobyric acid synthase CobQ2
MUL_4387	0	9	-1.798722	recombination protein RecR
MUL_4388	-1	13	-3.009572	hypothetical protein
MUL_4389	-1	17	-3.810290	N-acetylmuramoyl-L-alanine amidase
MUL_4391	2	17	-1.396148	hypothetical protein

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_4381	cloacin	40	5e-05	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 39.7 bits (92), Expect = 5e-05 Identities = 27/85 (31%), Positives = 37/85 (43%), Gaps = 2/85 (2%) Query: 517 NGGNGGKGANGNAALGANRNGGNGGIGGTGFIGGNGGNGGGGGAGGNGGNGAAGVSAGGA 576 +GG+G G + N NGG G+G G G + G+G GG +G+ GG Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGG--GASDGSGWSSENNPWGGGSGSGIHWGGG 59 Query: 577 GGAGGEGNGGTAGTGGKGGDGGTAS 601 G G G G +G G G +A Sbjct: 60 SGHGNGGGNGNSGGGSGTGGNLSAV 84 Score = 38.9 bits (90), Expect = 9e-05 Identities = 31/91 (34%), Positives = 38/91 (41%), Gaps = 3/91 (3%) Query: 426 LGGNGGHGGVGGGHTAGNGFNDGTTGAGGVGGVGGTGGVGGTG---GDGLHTALGRQGNG 482 + G G G G H+ N G TG G GG G G G + + G Sbjct: 1 MSGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGS 60 Query: 483 GAGGHGGSGNQGGYGGLSGDESSRAAQGATG 513 G G GG+GN GG G G+ S+ AA A G Sbjct: 61 GHGNGGGNGNSGGGSGTGGNLSAVAAPVAFG 91 Score = 37.8 bits (87), Expect = 2e-04 Identities = 28/80 (35%), Positives = 33/80 (41%) Query: 784 GQFNGGNGGKGGVGGTAGAGTTAGNGGHGGTGGTGGDGQTAAGVGGSGGTGGNGGRGGTG 843 G G N G G G T G G + G+G + GGSG GG G G Sbjct: 4 GDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHG 63 Query: 844 GAGGTGVTGGDGGRGGSGGA 863 GG G +GG G GG+ A Sbjct: 64 NGGGNGNSGGGSGTGGNLSA 83 Score = 37.4 bits (86), Expect = 3e-04 Identities = 30/89 (33%), Positives = 38/89 (42%) Query: 486 GHGGSGNQGGYGGLSGDESSRAAQGATGNDGNGGNGGKGANGNAALGANRNGGNGGIGGT 545 G G G+ G SG+ + G + G+G N G+ GG G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 546 GFIGGNGGNGGGGGAGGNGGNGAAGVSAG 574 G GGNG +GGG G GGN AA V+ G Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFG 91 Score = 36.6 bits (84), Expect = 5e-04 Identities = 30/85 (35%), Positives = 37/85 (43%), Gaps = 1/85 (1%) Query: 741 AGGAGANETYGGSGGSGGAAGNGGVGGLNGVGGNGGVGGHGGNGQFNGGNGGKGGVGGTA 800 +GG G G SG G G+ G G + G G N + GG+G GG + Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGG-GASDGSGWSSENNPWGGGSGSGIHWGGGS 60 Query: 801 GAGTTAGNGGHGGTGGTGGDGQTAA 825 G G GNG GG GTGG+ A Sbjct: 61 GHGNGGGNGNSGGGSGTGGNLSAVA 85 Score = 36.2 bits (83), Expect = 6e-04 Identities = 28/79 (35%), Positives = 31/79 (39%), Gaps = 1/79 (1%) Query: 810 GHGGTGGTGGDGQTAAGV-GGSGGTGGNGGRGGTGGAGGTGVTGGDGGRGGSGGAGGDGA 868 G G G G T+ + GG G G GG G G G G GG G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 869 GTCAGNGGNGGLGGAGGNG 887 G GNG +GG G GGN Sbjct: 63 GNGGGNGNSGGGSGTGGNL 81 Score = 35.8 bits (82), Expect = 7e-04 Identities = 33/104 (31%), Positives = 38/104 (36%), Gaps = 7/104 (6%) Query: 627 NVGGHGGNGGTGGAAGTGGHGGNGGDGGNGGINANGAAGGAGGNGGAGGGSTLLANGHGG 686 N G H +G G G GG DG N GG+G GGGS G G Sbjct: 10 NTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNG 69 Query: 687 AGGNGGNGGIGGENASNRGATG-------GAGGVGGIGGAGSLS 723 G G G + A G GAGG+ AG+LS Sbjct: 70 NSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALS 113 Score = 35.5 bits (81), Expect = 0.001 Identities = 27/86 (31%), Positives = 34/86 (39%), Gaps = 2/86 (2%) Query: 579 AGGEGNGGTAGTGGKGGDGGTASDDGVGGDGGSGGRGGDGNTRYTPRGNVGGHGGNGGTG 638 +GG+G G G G+ G G S G G ++ P G G G + G G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGW--SSENNPWGGGSGSGIHWGGG 59 Query: 639 GAAGTGGHGGNGGDGGNGGINANGAA 664 G GG GN G G G N + A Sbjct: 60 SGHGNGGGNGNSGGGSGTGGNLSAVA 85 Score = 35.5 bits (81), Expect = 0.001 Identities = 32/89 (35%), Positives = 36/89 (40%), Gaps = 6/89 (6%) Query: 512 TGNDGNGGNGGKGANGNAALGA------NRNGGNGGIGGTGFIGGNGGNGGGGGAGGNGG 565 T + NGG G G G A+ G+ N GG G G G GNGGG G G G Sbjct: 16 TSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNGNSGGGS 75 Query: 566 NGAAGVSAGGAGGAGGEGNGGTAGTGGKG 594 +SA A A G T G GG Sbjct: 76 GTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 35.5 bits (81), Expect = 0.001 Identities = 29/73 (39%), Positives = 32/73 (43%), Gaps = 8/73 (10%) Query: 829 GSGGTGGNGGRGGTGGAGGTGVTGGDGGRGGSGGAG--------GDGAGTCAGNGGNGGL 880 G G G N G T G G TG G G S G+G G G+G+ GG G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 881 GGAGGNGGHGGTS 893 G GGNG GG S Sbjct: 63 GNGGGNGNSGGGS 75 Score = 33.5 bits (76), Expect = 0.004 Identities = 26/89 (29%), Positives = 30/89 (33%) Query: 368 GVGGAGGEGGLIKGNGGAGGADGIGGTGGIGGDGSRGDTPLGPFNVDGHSGGDGGRGGLG 427 G G G G +G G G GG DGS + P+ SG G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 428 GNGGHGGVGGGHTAGNGFNDGTTGAGGVG 456 GNGG G GG + G G Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFG 91 Score = 33.1 bits (75), Expect = 0.005 Identities = 29/96 (30%), Positives = 37/96 (38%), Gaps = 10/96 (10%) Query: 548 IGGNGGNGGGGGAGGNGGNGAAGVSAGGAGGAGGEGNGGTAGTGGKGGDGGTASDDGVGG 607 + G G G GA GN G + G GG +G+G ++ GG G+ G G Sbjct: 1 MSGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGS 60 Query: 608 DGGSGGRGGDGNTRYTPRGNVGGHGGNGGTGGAAGT 643 G+GG GN GG G GG A Sbjct: 61 GHGNGGGN----------GNSGGGSGTGGNLSAVAA 86 Score = 33.1 bits (75), Expect = 0.005 Identities = 39/119 (32%), Positives = 46/119 (38%), Gaps = 8/119 (6%) Query: 709 GAGGVGGIGGAGSLS--IRGGGYGGLGGNGGTGGAGGAGANETYGGSGGSGGAAGNGGVG 766 G G G GA S S I GG G G G + G+G + N +GG GSG GG Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGG--GSGSGIHWGGGS 60 Query: 767 GLNGVGGNGGVGGHGGNGQFNGGNGGKGGVGGTAGAGTTAGNGGHGGTGGTGGDGQTAA 825 G GNGG G+ G G GGN G + G G +AA Sbjct: 61 G----HGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAA 115 Score = 32.8 bits (74), Expect = 0.007 Identities = 33/116 (28%), Positives = 42/116 (36%) Query: 630 GHGGNGGTGGAAGTGGHGGNGGDGGNGGINANGAAGGAGGNGGAGGGSTLLANGHGGAGG 689 G G G GA T G+ G G G A+ +G + N GGGS + GG+G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 690 NGGNGGIGGENASNRGATGGAGGVGGIGGAGSLSIRGGGYGGLGGNGGTGGAGGAG 745 G G S G A G +LS G G + + G A A Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAAIAD 118 Score = 32.4 bits (73), Expect = 0.010 Identities = 28/87 (32%), Positives = 35/87 (40%), Gaps = 5/87 (5%) Query: 538 GNGGIGGTGFIGGNGGNGGGGGAGGNGGNGAAGVSAGGAGGAGGEGNGGTAGTGGKGGDG 597 G G G GN G G G G + +G S+ GG G+G G G G+G Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNG 65 Query: 598 GTASDDGVGGDGGSGGRGGDGNTRYTP 624 G G G GG G GG+ + P Sbjct: 66 G-----GNGNSGGGSGTGGNLSAVAAP 87 Score = 31.6 bits (71), Expect = 0.014 Identities = 37/106 (34%), Positives = 40/106 (37%), Gaps = 22/106 (20%) Query: 641 AGTGGHGGNGGDGGNGGINANGAAGGAGGNGGAGGGSTLLANGHGGAGGNGGNGGIGGEN 700 +G G G N G G N NG G G GGA GS G EN Sbjct: 2 SGGDGRGHNTGAHSTSG-NINGGPTGLGVGGGASDGS-----------------GWSSEN 43 Query: 701 ASNRGATGGAGGVGGIGGAGSLSIRGGGYGGLGGNGGTGGAGGAGA 746 G +G GG G G+ GGG G GG GTGG A A Sbjct: 44 NPWGGGSGSGIHWGGGSGHGN----GGGNGNSGGGSGTGGNLSAVA 85 Score = 31.6 bits (71), Expect = 0.016 Identities = 27/80 (33%), Positives = 31/80 (38%), Gaps = 4/80 (5%) Query: 316 GRGSDGGAGGKGGNAGDYGHGGAGGTGGQGGTGGAGLTPGDKGFQGGLGGSGGVGGAGGE 375 GRG + GA GN G G G G+G + G G GG G G Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNG 65 Query: 376 GGLIKGNGGAGGADGIGGTG 395 G GNG +GG G GG Sbjct: 66 G----GNGNSGGGSGTGGNL 81 Score = 31.6 bits (71), Expect = 0.017 Identities = 32/100 (32%), Positives = 39/100 (39%), Gaps = 11/100 (11%) Query: 260 GDGGAGAPGAASFDPNVAGGAGGAGGDAGKIGDGGRGGDGGHGATGTAGDATHLEGGRGS 319 GDG GA S N+ GG G G G G +G + + GG GS Sbjct: 4 GDGRGHNTGAHSTSGNINGGPTGLG-----------VGGGASDGSGWSSENNPWGGGSGS 52 Query: 320 DGGAGGKGGNAGDYGHGGAGGTGGQGGTGGAGLTPGDKGF 359 GG G+ G+G +GG G GG A P GF Sbjct: 53 GIHWGGGSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGF 92 Score = 31.2 bits (70), Expect = 0.021 Identities = 26/78 (33%), Positives = 32/78 (41%), Gaps = 1/78 (1%) Query: 770 GVGGNGGVGGHGGNGQFNGGNGGKGGVGGTAGAGTTAGNGGHGGTGGTGGDGQTAAGVGG 829 G G G H +G NGG G GVGG A G+ + + GG+G G G Sbjct: 4 GDGRGHNTGAHSTSGNINGGPTG-LGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 830 SGGTGGNGGRGGTGGAGG 847 G G GG+G G Sbjct: 63 GNGGGNGNSGGGSGTGGN 80 Score = 31.2 bits (70), Expect = 0.021 Identities = 29/82 (35%), Positives = 31/82 (37%), Gaps = 2/82 (2%) Query: 682 NGHGGAGGNGGNGGIGGENASNRGATGGAGGVGGIGGAGSLSIRGGGYGGLGGNGGTGGA 741 +G G G N G G N G TG G G G+G S GG G GG Sbjct: 2 SGGDGRGHNTGAHSTSGN--INGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGG 59 Query: 742 GGAGANETYGGSGGSGGAAGNG 763 G G G SGG G GN Sbjct: 60 SGHGNGGGNGNSGGGSGTGGNL 81 Score = 30.8 bits (69), Expect = 0.025 Identities = 27/101 (26%), Positives = 34/101 (33%) Query: 568 AAGVSAGGAGGAGGEGNGGTAGTGGKGGDGGTASDDGVGGDGGSGGRGGDGNTRYTPRGN 627 + G G GA G G G GG + G + G G + Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 628 VGGHGGNGGTGGAAGTGGHGGNGGDGGNGGINANGAAGGAG 668 G GGNG +GG +GTGG+ G A G G Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG 102 Score = 30.8 bits (69), Expect = 0.032 Identities = 30/86 (34%), Positives = 37/86 (43%), Gaps = 6/86 (6%) Query: 792 GKGGVGGTAGAGTTAGNGGHGGTGGTGGDGQTAAGVGGSGGTGGNGGRGGTGGAGGTGVT 851 G G G GA +T+GN +GG G G G + G G S GG G+G Sbjct: 3 GGDGRGHNTGAHSTSGNI-NGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIH-----W 56 Query: 852 GGDGGRGGSGGAGGDGAGTCAGNGGN 877 GG G G GG G G G+ G + Sbjct: 57 GGGSGHGNGGGNGNSGGGSGTGGNLS 82 Score = 30.1 bits (67), Expect = 0.042 Identities = 27/83 (32%), Positives = 33/83 (39%), Gaps = 2/83 (2%) Query: 814 TGGTGGDGQTAAGVGGSGGTGGNGGRGGTGGA--GGTGVTGGDGGRGGSGGAGGDGAGTC 871 +GG G T A GG G G GGA G + + GGSG G G+ Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 872 AGNGGNGGLGGAGGNGGHGGTST 894 GNGG G G G G ++ Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAV 84
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_4384	DHBDHDRGNASE	79	2e-19	2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase signature. Length = 261 Score = 78.6 bits (193), Expect = 2e-19 Identities = 68/254 (26%), Positives = 104/254 (40%), Gaps = 22/254 (8%) Query: 14 VVLITGGSRGLGRQMAFAAARCGANVVIASRNLDNCVATATEIESETGRSALAYQVHVGR 73 + ITG ++G+G +A A GA++ N + + R A A+ V Sbjct: 10 IAFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSS-LKAEARHAEAFPADVRD 68 Query: 74 WDQLDGLVAASYERFGKIDTLINNAG---MSPLYDKLSDVTEKLFDAVLNLNLKGPFRLS 130 +D + A G ID L+N AG + LSD + ++A ++N G F S Sbjct: 69 SAAIDEITARIEREMGPIDILVNVAGVLRPGLI-HSLSD---EEWEATFSVNSTGVFNAS 124 Query: 131 ALVGERMVAADGGSIINVSSAGSLRPSADIIPYAAAKAGLNAMTEGLARAFGPT-VRVNT 189 V + M+ GSI+ V S + P + YA++KA T+ L +R N Sbjct: 125 RSVSKYMMDRRSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLELAEYNIRCNI 184 Query: 190 LMAGPFLTDVSKAWNL----DAATQNPFGHLA-------LRRAGNPPEIVGAALFLASDA 238 + G T+ W+L + A Q G L L++ P +I A LFL S Sbjct: 185 VSPGS--TETDMQWSLWADENGAEQVIKGSLETFKTGIPLKKLAKPSDIADAVLFLVSGQ 242 Query: 239 SSFTTGSILRADGG 252 + T L DGG Sbjct: 243 AGHITMHNLCVDGG 256
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_4386	HTHTETR	51	3e-10	TetR bacterial regulatory protein HTH signature.
>HTHTETR#TetR bacterial regulatory protein HTH signature. Length = 215 Score = 50.8 bits (121), Expect = 3e-10 Identities = 24/121 (19%), Positives = 43/121 (35%), Gaps = 2/121 (1%) Query: 16 RQREATEEVERILAAAVRVMERAAPEPPRVSDIVAEAGSSNKAFYRYFAGKDDLILAVME 75 ++EA E + IL A+R+ + + +I AG + A Y +F K DL + E Sbjct: 5 TKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSEIWE 64 Query: 76 RGVAIVVSYLGHQMAKESRPDTKIARWIEGTLAQVADPHLISMSRAAAGQLSNWLATQRE 135 + + AK P ++ E + + R + + E Sbjct: 65 LSESNIGELELEYQAK--FPGDPLSVLREILIHVLESTVTEERRRLLMEIIFHKCEFVGE 122 Query: 136 M 136 M Sbjct: 123 M 123
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_4387	DHBDHDRGNASE	31	0.004	2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase signature. Length = 261 Score = 31.2 bits (70), Expect = 0.004 Identities = 16/64 (25%), Positives = 25/64 (39%), Gaps = 2/64 (3%) Query: 137 QGDWVVVLGAAGGVGLAAVDLAVAMGARVLAAASSPEKLGLCRQRGAEAVVDYDQEDLKL 196 +G + GAA G+G A + GA + A +PEKL + E Sbjct: 7 EGKIAFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVS--SLKAEARHAEAFPA 64 Query: 197 RIRE 200 +R+ Sbjct: 65 DVRD 68
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_4391	HTHFIS	104	3e-28	FIS bacterial regulatory protein HTH signature.
>HTHFIS#FIS bacterial regulatory protein HTH signature. Length = 484 Score = 104 bits (262), Expect = 3e-28 Identities = 35/118 (29%), Positives = 65/118 (55%) Query: 10 SVLVVDDEPVLADMVSMALRYEGWNIATASDGASAIASARAERPDVVVLDVMLPDMSGLE 69 ++LV DD+ + +++ AL G+++ S+ A+ A D+VV DV++PD + + Sbjct: 5 TILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAFD 64 Query: 70 VLHRLRKENPRLPVLLLTAKDAVEDRIAGLTAGGDDYVTKPFSIEEIVLRLRALLRRT 127 +L R++K P LPVL+++A++ I G DY+ KPF + E++ + L Sbjct: 65 LLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAEP 122

MUL_4412

MUL_4419

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
MUL_4412	-1	14	-2.400744	hypothetical protein
MUL_4413	1	10	-0.704252	hypothetical protein
MUL_4415	1	10	-0.704140	PE family protein
MUL_4416	0	8	-0.454287	hypothetical protein
MUL_4417	2	11	0.911019	hypothetical protein
MUL_4418	1	11	1.360546	ATP-dependent DNA ligase
MUL_4419	1	10	0.746897	hypothetical protein

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_4412	IGASERPTASE	30	0.023	IgA-specific serine endopeptidase (S6) signature.
>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature. Length = 1541 Score = 29.6 bits (66), Expect = 0.023 Identities = 17/50 (34%), Positives = 20/50 (40%), Gaps = 3/50 (6%) Query: 283 VTFDEDRHRAHTGNGAQVLATLRNTAINLHRLNGADNIAEACRITALTAN 332 V E+ H TGN L N I+ LN ADN + LT N Sbjct: 846 VRLTENSHWHLTGNSDVHQLDLANGHIH---LNSADNSNNVTKYNTLTVN 892
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_4415	IGASERPTASE	30	0.020	IgA-specific serine endopeptidase (S6) signature.
>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature. Length = 1541 Score = 29.6 bits (66), Expect = 0.020 Identities = 17/51 (33%), Positives = 20/51 (39%), Gaps = 3/51 (5%) Query: 283 VTFDEDRHRAHTGNGAQVLATLRNTAINLHRLNGADNIAEACRITALTANR 333 V E+ H TGN L N I+ LN ADN + LT N Sbjct: 846 VRLTENSHWHLTGNSDVHQLDLANGHIH---LNSADNSNNVTKYNTLTVNS 893
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_4417	DHBDHDRGNASE	64	3e-14	2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase signature. Length = 261 Score = 63.9 bits (155), Expect = 3e-14 Identities = 52/190 (27%), Positives = 86/190 (45%), Gaps = 9/190 (4%) Query: 6 ILIAGASSGLGAGMARAFAARGRDLALCARRTDRLEELKSELAQ--KHPEITIAIAELDV 63 I GA+ G+G +AR A++G +A ++LE++ S L +H E DV Sbjct: 11 AFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHAEAF----PADV 66 Query: 64 NDHDQVPKVFAELRDELGGIDRVIVNAGIGKGAPLGSGKLWANKATIETNLVAALVQIET 123 D + ++ A + E+G ID ++ AG+ + + S +AT N + Sbjct: 67 RDSAAIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGVFNASRS 126 Query: 124 ALEMFHKSGSGHLVLISSVLASKGVPGVK-AAYAASKAGLSSLGESLRAEYDKSPITVSV 182 + SG +V + S A GVP AAYA+SKA + L E + I ++ Sbjct: 127 VSKYMMDRRSGSIVTVGSNPA--GVPRTSMAAYASSKAAAVMFTKCLGLELAEYNIRCNI 184 Query: 183 MEPGYIESEM 192 + PG E++M Sbjct: 185 VSPGSTETDM 194
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_4419	cloacin	46	2e-07	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 45.9 bits (108), Expect = 2e-07 Identities = 35/101 (34%), Positives = 43/101 (42%), Gaps = 4/101 (3%) Query: 207 GLGGNGGTVGTGQSTNGGAGGDGGSGGSAGLFGGGGAGALGGDGGNGVGSDGSGGGAGSG 266 G G N G T + NGG G G GG++ G G G G G G G+G Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNG 65 Query: 267 GDGGNGGFFYGDGGNGADAGSPGAGQSSFGSLGIAGEGDGG 307 G GN G G GGN + +P A FG ++ G GG Sbjct: 66 GGNGNSGGGSGTGGNLSAVAAPVA----FGFPALSTPGAGG 102 Score = 37.4 bits (86), Expect = 8e-05 Identities = 31/83 (37%), Positives = 37/83 (44%), Gaps = 3/83 (3%) Query: 185 GNGGNGGNAGLLQGVAG-NGAAGGLGGNGG-TVGTGQSTNGGAGGDGGSGGSAGLFGGGG 242 G G G N G NG GLG GG + G+G S+ G GGSG GG G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWG-GGSGSGIHWGGGSG 61 Query: 243 AGALGGDGGNGVGSDGSGGGAGS 265 G GG+G +G GS G + Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAV 84 Score = 35.8 bits (82), Expect = 2e-04 Identities = 35/113 (30%), Positives = 40/113 (35%), Gaps = 16/113 (14%) Query: 225 AGGDG-GSGGSAGLFGGGGAGALGGDGGNGVGSDGSGGGAGSGGDGGNGGFFYGDGGNGA 283 +GGDG G A G G G G G SDGSG + + GG G GG Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 284 DAGSPGAGQSSFGSLGIAGEGDGGDGGNAFLIGNGGNGGAAAAFGFPGFGGNG 336 G G+G GG + GN A AFGFP G Sbjct: 62 HGN---------------GGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPG 99 Score = 32.8 bits (74), Expect = 0.002 Identities = 31/87 (35%), Positives = 39/87 (44%), Gaps = 8/87 (9%) Query: 140 GRGGSGGVGQKGGNGGSAGLWGNGGNGGLG-GEGVQGGPGHPGQAGGNGGNGGNAGLLQG 198 GRG + G GN NGG GLG G G G G + GG G+ G Sbjct: 6 GRGHNTGAHSTSGNI-------NGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGG 58 Query: 199 VAGNGAAGGLGGNGGTVGTGQSTNGGA 225 +G+G GG G +GG GTG + + A Sbjct: 59 GSGHGNGGGNGNSGGGSGTGGNLSAVA 85 Score = 31.6 bits (71), Expect = 0.005 Identities = 28/86 (32%), Positives = 33/86 (38%), Gaps = 12/86 (13%) Query: 120 NGADGAAGTGQNGGDGGWLIGRGGSGGVGQKGGNGGSAGLWGNGGNGGLGGEGVQGGPGH 179 N + NGG G +G G S G G N WG G G+ G G Sbjct: 10 NTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNP----WGGGSGSGIHWGGGSG---- 61 Query: 180 PGQAGGNGGNGGNAGLLQGVAGNGAA 205 GNGG GN+G G GN +A Sbjct: 62 ----HGNGGGNGNSGGGSGTGGNLSA 83

MUL_4500

MUL_4507

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
MUL_4500	0	14	-0.365003	O-methyltransferase
MUL_4501	-2	10	0.205838	hypothetical protein
MUL_4502	-3	10	-0.325434	hypothetical protein
MUL_4503	-2	10	-0.847484	**putative aminotransferase
MUL_4504	-2	11	-1.526386	hypothetical protein
MUL_4506	-2	13	-2.390940	hypothetical protein
MUL_4507	0	14	-0.298527	fusion of enoyl-CoA hydratase, EchA21 and

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_4500	PF03544	36	5e-04	Gram-negative bacterial tonB protein
>PF03544#Gram-negative bacterial tonB protein Length = 243 Score = 36.1 bits (83), Expect = 5e-04 Identities = 23/100 (23%), Positives = 29/100 (29%), Gaps = 11/100 (11%) Query: 49 TEPAVVKPAAAP-AKPAPAPAPAKPAAGPPAAGNGSPAAAPSAKPAAAPAKAPAPPPA-- 105 PA ++P A P P P P P AP P P P P Sbjct: 55 VAPADLEPPQAVQPPPEPVVEPEPEPEPIPEP----PKEAPVVIEKPKPKPKPKPKPVKK 110 Query: 106 ----EGDEMQVLRGAAAAVVKNMSASLDVPTATSVRAVPA 141 + D V A+ A TAT+ + P Sbjct: 111 VEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPV 150 Score = 34.6 bits (79), Expect = 0.002 Identities = 24/139 (17%), Positives = 36/139 (25%), Gaps = 5/139 (3%) Query: 24 DDPSSVDPSWHEFLVDYNPESTQEATEPAVVKPAAAPAKPAP--APAPAKPAAGPPAAGN 81 P+ + P + AV P +P P P P P P Sbjct: 39 QVIELPAPAQPISVTMVAPADLEPP--QAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEK 96 Query: 82 GSPAAAPSAKPAAA-PAKAPAPPPAEGDEMQVLRGAAAAVVKNMSASLDVPTATSVRAVP 140 P P KP P E A A + +A+ + A Sbjct: 97 PKPKPKPKPKPVKKVEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASG 156 Query: 141 AKLLIDNRIVINNQLKRNR 159 + L N+ + + R Sbjct: 157 PRALSRNQPQYPARAQALR 175
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_4501	DHBDHDRGNASE	78	5e-19	2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase signature. Length = 261 Score = 77.8 bits (191), Expect = 5e-19 Identities = 53/201 (26%), Positives = 90/201 (44%), Gaps = 2/201 (0%) Query: 2 EGFAGKVAVVTGAGSGIGRALAIELARSGAKLAISDVDTEGLAQTEKLVTALGAEVKTDR 61 +G GK+A +TGA GIG A+A LA GA +A D + E L + + A + Sbjct: 4 KGIEGKIAFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHAEAFP 63 Query: 62 LDVTERETFLAYADAVNEHFGKVNQIYNNAGIGHTGDVEVCAFKDIDRVMDVDFGGVLNG 121 DV + + G ++ + N AG+ G + + ++ + V+ GV N Sbjct: 64 ADVRDSAAIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGVFNA 123 Query: 122 TKTFLPYLIASGDGHVINVSSVFGLFSVSGQAAYNAAKFAVRGFTEALRQEMILAGHPVG 181 +++ Y++ G ++ V S + AAY ++K A FT+ L E LA + + Sbjct: 124 SRSVSKYMMDRRSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLE--LAEYNIR 181 Query: 182 VTTVHPGGIKTAIARNATAAE 202 V PG +T + + A E Sbjct: 182 CNIVSPGSTETDMQWSLWADE 202
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_4506	IGASERPTASE	30	0.018	IgA-specific serine endopeptidase (S6) signature.
>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature. Length = 1541 Score = 30.0 bits (67), Expect = 0.018 Identities = 17/51 (33%), Positives = 20/51 (39%), Gaps = 3/51 (5%) Query: 283 VTFDEDRHRAHTGNGAQVLATLRNTAINLHRLNGADNIAEACRITALTANR 333 V E+ H TGN L N I+ LN ADN + LT N Sbjct: 846 VRLTENSHWHLTGNSDVHQLDLANGHIH---LNSADNSNNVTKYNTLTVNS 893
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_4507	PHPHTRNFRASE	31	0.009	Phosphoenolpyruvate-protein phosphotransferase sign...
>PHPHTRNFRASE#Phosphoenolpyruvate-protein phosphotransferase signature. Length = 572 Score = 30.9 bits (70), Expect = 0.009 Identities = 18/111 (16%), Positives = 39/111 (35%), Gaps = 6/111 (5%) Query: 216 IRGLAADHGRALSYGLRIH----VITRDTAEEAWRVADRLLAGIDPA--DIERMQANLAR 269 I G+AA G A++ I + + + ++L A ++ + ++ ++ Sbjct: 5 ITGIAASSGVAIAKAFIHLEPNVDIEKTSITDVSTEIEKLTAALEKSKEELRAIKDQTEA 64 Query: 270 SESEGQRRMAELHGGVLDQLEIAPNLWAGVGLVRGGAGTALVGSHQEVAER 320 S + + H VLD E+ + + + A AL Sbjct: 65 SMGADKAEIFAAHLLVLDDPELVDGIKGKIENEQMNAEYALKEVSDMFVSM 115

MUL_4857

MUL_4865

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
MUL_4857	-2	10	0.494382	hypothetical protein
MUL_4858	-1	10	0.748448	hypothetical protein
MUL_4860	1	14	1.020748	TetR family transcriptional regulator
MUL_4861	1	14	0.593557	hypothetical protein
MUL_4862	-1	15	0.009352	hypothetical protein
MUL_4863	0	14	0.440483	hypothetical protein
MUL_4864	-2	14	0.648542	oxidoreductase
MUL_4865	0	12	0.749715	hypothetical protein

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_4857	ISCHRISMTASE	31	3e-04	Isochorismatase signature.
>ISCHRISMTASE#Isochorismatase signature. Length = 312 Score = 30.8 bits (69), Expect = 3e-04 Identities = 20/57 (35%), Positives = 28/57 (49%) Query: 15 IDETDLIDGDATDLRDLGLDSVRFVLLMKRLGVDRESELPSRLAENLSIEGWVSELS 71 + ET D DL D GLDSVR + L+++ + LAE +IE W L+ Sbjct: 243 LQETPEDITDQEDLLDRGLDSVRIMTLVEQWRREGAEVTFVELAERPTIEEWQKLLT 299
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_4860	DHBDHDRGNASE	74	1e-17	2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase signature. Length = 261 Score = 73.9 bits (181), Expect = 1e-17 Identities = 49/184 (26%), Positives = 80/184 (43%), Gaps = 3/184 (1%) Query: 10 RAAIVTGASSGIGEEFARILSQRGYQVVLVARSADRLEALAGRL---GSDTHPLPADLSV 66 + A +TGA+ GIGE AR L+ +G + V + ++LE + L PAD+ Sbjct: 9 KIAFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHAEAFPADVRD 68 Query: 67 RSDRAGLVDRVAALGLVPDILINNAGLSTLGPVAKSVPEQEFNLAEVDVAAVVDLCSRFL 126 + + R+ DIL+N AG+ G + E+ V+ V + Sbjct: 69 SAAIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGVFNASRSVS 128 Query: 127 PAMVERGRGAVFNVASVAGFAPLPGQAAYGAAKAFVLSYTHSLRGELHGSGVSVTALSPG 186 M++R G++ V S P AAY ++KA + +T L EL + +SPG Sbjct: 129 KYMMDRRSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLELAEYNIRCNIVSPG 188 Query: 187 PVDT 190 +T Sbjct: 189 STET 192
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_4864	HTHTETR	63	2e-14	TetR bacterial regulatory protein HTH signature.
>HTHTETR#TetR bacterial regulatory protein HTH signature. Length = 215 Score = 62.7 bits (152), Expect = 2e-14 Identities = 34/203 (16%), Positives = 69/203 (33%), Gaps = 9/203 (4%) Query: 16 KVREAQRLRTRARVFDAAVAEIGRRGLAGADVAAIAAAAGVARGTFYFHFPTKEHVLVEL 75 + + + TR + D A+ ++G++ + IA AAGV RG Y+HF K + E+ Sbjct: 3 RKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSEI 62 Query: 76 -----ERAEELAIVAKLRDPTADSTDLVSVLSSLAHQVV--AVERRLGPLVFRDMLGLHF 128 EL + + + P + L +L + V R L ++F + Sbjct: 63 WELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVTEERRRLLMEIIFHKCEFVGE 122 Query: 129 APTRPVEDQLGEHPLAEFVIETIAQAQRADRVAPDADAGELGVIFLTGLFALLATGATTP 188 + + + +T+ A + D +I + L+ P Sbjct: 123 MAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMTRRAAIIMRGYISGLMENWLFAP 182 Query: 189 DARTAL--LNGFVTTVVHGMEAR 209 + +V ++ Sbjct: 183 QSFDLKKEARDYVAILLEMYLLC 205
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_4865	DHBDHDRGNASE	57	8e-12	2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase signature. Length = 261 Score = 57.4 bits (138), Expect = 8e-12 Identities = 60/279 (21%), Positives = 97/279 (34%), Gaps = 66/279 (23%) Query: 6 ITGSASGMGNATASRLWEAGHRVIGVDLDGADVVADLSTQQGRLRAAS----DV------ 55 ITG+A G+G A A L G + VD + + +S+ + R A DV Sbjct: 13 ITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHAEAFPADVRDSAAI 72 Query: 56 ------IAACDGRLDGAVLAAGLGPSPGPGRLHRIAQ--------VNYLGVVELLQARRP 101 I G +D V AG+ PG +H ++ VN GV ++ Sbjct: 73 DEITARIEREMGPIDILVNVAGV---LRPGLIHSLSDEEWEATFSVNSTGVFNASRSVSK 129 Query: 102 ALAAAERAKAVVIASNSTTTVPMVPRRSVRALLDHDADKAVRAVRLFGRAAPSLMYGASK 161 + V + SN PR S+ A Y +SK Sbjct: 130 YMMDRRSGSIVTVGSNPAGV----PRTSMAA------------------------YASSK 161 Query: 162 IAVSHWARRQAVLPEWAGSGVRLNALAPGAIMTPLLAEQLSVPTQAKAVR--------SF 213 A + + + E A +R N ++PG+ T + L Sbjct: 162 AAAVMFTK--CLGLELAEYNIRCNIVSPGSTETD-MQWSLWADENGAEQVIKGSLETFKT 218 Query: 214 PIPVGGFGEVTHMADWICFMLSDSADFLCGSVVFVDGGS 252 IP+ + + +AD + F++S A + + VDGG+ Sbjct: 219 GIPLKKLAKPSDIADAVLFLVSGQAGHITMHNLCVDGGA 257

MUL_5004

MUL_5016

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
MUL_5004	-2	13	2.385559	MCE-family protein Mce6C
MUL_5005	-1	13	2.730848	MCE-family protein Mce6B
MUL_5006	-2	12	3.307365	MCE-family protein Mce6A
MUL_5007	-2	11	3.635758	integral membrane protein YrbE6B
MUL_5008	0	11	2.616533	integral membrane protein YrbE6A
MUL_5010	-1	11	2.846801	long-chain-fatty-acid--CoA ligase
MUL_5011	0	8	2.728863	acyl carrier protein
MUL_5012	0	9	2.240029	hypothetical protein
MUL_5013	0	8	0.772657	ABC transporter ATP-binding protein
MUL_5014	-2	9	-0.435955	hypothetical protein
MUL_5015	0	11	0.536582	hypothetical protein
MUL_5016	-1	11	-0.700583	hypothetical protein

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_5004	HTHTETR	53	1e-10	TetR bacterial regulatory protein HTH signature.
>HTHTETR#TetR bacterial regulatory protein HTH signature. Length = 215 Score = 52.7 bits (126), Expect = 1e-10 Identities = 36/209 (17%), Positives = 69/209 (33%), Gaps = 15/209 (7%) Query: 1 MVRPAQTVRSERTREALRQAAVVRFLAQGVEETSAEQIAADAGVSLRTFYRHFRSKHDLL 60 M R + ++ TR+ + A+ F QGV TS +IA AGV+ Y HF+ K DL Sbjct: 1 MARKTKQ-EAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLF 59 Query: 61 FADYTGLH-----WFRAALDARPVD-EAIIDSVQTAIFAFPYDVEAVAKIATLRGGELDP 114 + P D +++ + + E + + + + Sbjct: 60 SEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVTEERRRLLMEIIFHKCEF 119 Query: 115 SRIVRHIQEVQADFAEVIAAQLLRRSCAAAGTSAQTP--DARVRTAVTARCIAAAVFGAM 172 + +Q+ Q + + R + A + T A + + G M Sbjct: 120 VGEMAVVQQAQRNLCL----ESYDRIEQTLKHCIEAKMLPADLMTRRAAIIMRGYISGLM 175 Query: 173 EVWMLGEDRSLGELARVCQLALETSRAGL 201 E W+ +L + + + Sbjct: 176 ENWLFAPQSF--DLKKEARDYVAILLEMY 202
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_5007	PF03544	39	2e-05	Gram-negative bacterial tonB protein
>PF03544#Gram-negative bacterial tonB protein Length = 243 Score = 38.8 bits (90), Expect = 2e-05 Identities = 18/103 (17%), Positives = 26/103 (25%) Query: 356 PPVPPPDIPEERLTLPPIPLQVPPAAPAPRQTQTAPPSNQHLPNQQPVVTPSRAPAAPAT 415 +P P P + P L+ P A P + P + P P Sbjct: 41 IELPAPAQPISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPK 100 Query: 416 TTQATQPPPATQSQPPPADGGAPPPAQAPEPEAPAPAEPAPGG 458 +P + PA E APA + Sbjct: 101 PKPKPKPVKKVEQPKRDVKPVESRPASPFENTAPARPTSSTAT 143 Score = 33.8 bits (77), Expect = 0.001 Identities = 15/94 (15%), Positives = 26/94 (27%), Gaps = 8/94 (8%) Query: 357 PVPPPDIPEERLTLPPIPLQVPPAAPAPRQTQTAPPSNQHLPNQQPVVTPSRAPAAPATT 416 PV P+ E + PP AP + P + P V + P + Sbjct: 72 PVVEPEPEPEPIPEPP--------KEAPVVIEKPKPKPKPKPKPVKKVEQPKRDVKPVES 123 Query: 417 TQATQPPPATQSQPPPADGGAPPPAQAPEPEAPA 450 A+ ++P + A + Sbjct: 124 RPASPFENTAPARPTSSTATAATSKPVTSVASGP 157
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_5010	OMADHESIN	28	0.039	Yersinia outer membrane adhesin signature.
>OMADHESIN#Yersinia outer membrane adhesin signature. Length = 455 Score = 27.9 bits (61), Expect = 0.039 Identities = 19/62 (30%), Positives = 26/62 (41%) Query: 36 AQAQAFARGGLIRPAMLVHSIATRASQAAEVVAAVLTVSAHEVVGIHEVQVGELENRCDD 95 + GGL A +HSIA A+ A AAV + G++ V +G L D Sbjct: 53 VRPPVPGAGGLNASAKGIHSIAIGATAEAAKGAAVAVGAGSIATGVNSVAIGPLSKALGD 112 Query: 96 EA 97 A Sbjct: 113 SA 114
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_5012	cloacin	30	0.008	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 30.5 bits (68), Expect = 0.008 Identities = 30/117 (25%), Positives = 50/117 (42%), Gaps = 21/117 (17%) Query: 148 ESVVVTDSTGAEPISVVDLLAARPDPF------CEIESSLLRHLTTDHQDVVARLVSRLP 201 + V+ S GA ++ D++AA PF + L + D ++++++V+ LP Sbjct: 101 GGLAVSISAGALSAAIADIMAALKGPFKFGLWGVALYGVLPSQIAKDDPNMMSKIVTSLP 160 Query: 202 A-PLRRGEVRPLGLDRYGVRFRIESNDGDRDIRLPFHRPVDDMHGLRQAIRVLLGCP 257 A + V L LD+ V + R VDD+ RQ I V+ G P Sbjct: 161 ADDITESPVSSLPLDKATVNVNV--------------RVVDDVKDERQNISVVSGVP 203
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_5013	PF03544	36	2e-04	Gram-negative bacterial tonB protein
>PF03544#Gram-negative bacterial tonB protein Length = 243 Score = 35.7 bits (82), Expect = 2e-04 Identities = 19/69 (27%), Positives = 25/69 (36%), Gaps = 1/69 (1%) Query: 13 QPPIPPQSEPSQVIRRDTLPLRRPAEPSGAPPRQPTSAPPPPKPATGPVGPRRRRRTPTP 72 +PP Q P V+ + P P P AP P P P PV + + Sbjct: 61 EPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPK-PKPKPVKKVEQPKRDVK 119 Query: 73 APPPRPAAP 81 RPA+P Sbjct: 120 PVESRPASP 128 Score = 29.2 bits (65), Expect = 0.027 Identities = 19/78 (24%), Positives = 27/78 (34%), Gaps = 5/78 (6%) Query: 11 RPQPPIPPQSE-PSQVIRRDTLPLRRPAEPSGAPPRQPTSAPPPPKPATGPVGPRRRRRT 69 P P P + ++ P EP P +P P PPK A + Sbjct: 43 LPAPAQPISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAP----VVIEKPK 98 Query: 70 PTPAPPPRPAAPKARRKK 87 P P P P+P + K+ Sbjct: 99 PKPKPKPKPVKKVEQPKR 116
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_5016	ANTHRAXTOXNA	37	8e-05	Anthrax toxin LF subunit signature.
>ANTHRAXTOXNA#Anthrax toxin LF subunit signature. Length = 800 Score = 37.4 bits (86), Expect = 8e-05 Identities = 10/20 (50%), Positives = 17/20 (85%) Query: 198 AVAFAYYGLPEHRSTLQLWA 217 ++AF+YY P+HR+ L+L+A Sbjct: 242 SLAFSYYFAPDHRTVLELYA 261

MUL_5037

MUL_5046

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
MUL_5037	-2	9	-1.809560	ATPase
MUL_5039	0	19	-2.422703	transcriptional regulator
MUL_5040	0	18	-2.056349	hypothetical protein
MUL_5045	1	22	-1.194609	hypothetical protein
MUL_5046	2	22	-1.878924	lipid-transfer protein Ltp1

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_5037	ARGREPRESSOR	30	0.032	Bacterial arginine repressor signature.
>ARGREPRESSOR#Bacterial arginine repressor signature. Length = 149 Score = 30.2 bits (68), Expect = 0.032 Identities = 17/81 (20%), Positives = 30/81 (37%), Gaps = 4/81 (4%) Query: 228 RFSTNTFPSWPLAHPFRRIAHNGE---INTVTGNENWM-RAREALIKTDVFGTEANVEKL 283 RF+ + L F +I + T+ GN + + L ++ GT + + Sbjct: 69 RFNPLSKLKRSLMDAFVKIDSASHLIVLKTMPGNAQAIGALMDNLDWEEIMGTICGDDTI 128 Query: 284 FPICTPGASDTARFDEVLELL 304 IC ++LELL Sbjct: 129 LIICRTHDDTKVVQKKILELL 149
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_5039	IGASERPTASE	30	0.020	IgA-specific serine endopeptidase (S6) signature.
>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature. Length = 1541 Score = 29.6 bits (66), Expect = 0.020 Identities = 17/51 (33%), Positives = 20/51 (39%), Gaps = 3/51 (5%) Query: 283 VTFDEDRHRAHTGNGAQVLATLRNTAINLHRLNGADNIAEACRITALTANR 333 V E+ H TGN L N I+ LN ADN + LT N Sbjct: 846 VRLTENSHWHLTGNSDVHQLDLANGHIH---LNSADNSNNVTKYNTLTVNS 893
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_5045	VACCYTOTOXIN	28	0.039	Helicobacter pylori vacuolating cytotoxin signature.
>VACCYTOTOXIN#Helicobacter pylori vacuolating cytotoxin signature. Length = 1291 Score = 28.5 bits (63), Expect = 0.039 Identities = 11/17 (64%), Positives = 14/17 (82%), Gaps = 1/17 (5%) Query: 118 DGGWR-GNAAQAYWVQN 133 DGGW GNAA+ YWV++ Sbjct: 110 DGGWDWGNAARHYWVKD 126
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
MUL_5046	IGASERPTASE	30	0.018	IgA-specific serine endopeptidase (S6) signature.
>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature. Length = 1541 Score = 30.0 bits (67), Expect = 0.018 Identities = 17/51 (33%), Positives = 20/51 (39%), Gaps = 3/51 (5%) Query: 283 VTFDEDRHRAHTGNGAQVLATLRNTAINLHRLNGADNIAEACRITALTANR 333 V E+ H TGN L N I+ LN ADN + LT N Sbjct: 846 VRLTENSHWHLTGNSDVHQLDLANGHIH---LNSADNSNNVTKYNTLTVNS 893


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