:: PredictBias ::

A) Input parameters
Genome	Streptobacillus_moniliformis_DSM_12112_uid29309_CP001779.gbk	Threshold dinucleotide bias	2
Threshold codon bias	4	Threshold %GC bias	3
E-value (RPSBlast)	0.05	Genome (non-pathogenic)

B) Compare a potential GI or PAI in related non-pathogenic sp. (phylogenetic tree)
Potential GI or PAI start end
Select Organism

C) Potential GIs and PAIs in CP001779 (download)

S.No

Start

End

Bias

Virulence

Insertion elements

Prediction

Smon_0209

Smon_0243

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
Smon_0209	2	12	1.948413	phosphotransferase system lactose/cellobiose-
Smon_0210	2	11	2.512006	hypothetical protein
Smon_0211	4	13	2.710382	glutamyl-tRNA synthetase
Smon_0212	5	13	2.389914	transcriptional regulator, TetR family
Smon_0213	5	12	2.779346	ribosomal protein S2
Smon_0214	3	11	2.092035	translation elongation factor Ts
Smon_0215	1	11	0.884345	uridylate kinase
Smon_0216	1	11	-0.467839	ribosome recycling factor
Smon_0217	2	10	-0.052447	cell shape determining protein MreB/Mrl
Smon_0218	1	11	0.107763	transposase IS200-family protein
Smon_0219	0	11	0.654570	TPR repeat-containing protein
Smon_0220	2	10	0.752562	hypothetical protein
Smon_0221	1	10	0.043853	putative PTS IIA-like nitrogen-regulatory
Smon_0222	0	11	2.431553	periplasmic solute binding protein
Smon_0223	0	13	2.643494	ABC transporter related protein
Smon_0224	0	14	2.409333	ABC-3 protein
Smon_0225	0	14	2.293611	ABC-3 protein
Smon_0226	0	14	2.197340	hypothetical protein
Smon_0227	0	14	2.539682	YadA domain protein
Smon_0228	3	15	1.346611	YadA domain protein
Smon_0230	4	18	-0.952310	hypothetical protein
Smon_0231	1	10	0.812131	Auxin Efflux Carrier
Smon_0232	-2	12	2.246639	Chromate transporter
Smon_0233	-2	12	2.225988	Chromate transporter
Smon_0234	-1	10	2.580054	*****conserved hypothetical protein
Smon_0235	0	11	2.045739	hypothetical protein
Smon_0236	-1	11	2.310623	valyl-tRNA synthetase
Smon_0237	1	16	1.432231	OmpA/MotB domain protein
Smon_0238	3	17	-1.851835	putative transcriptional acitvator, Baf family
Smon_0239	1	19	-2.763837	hypothetical protein
Smon_0240	1	16	-4.202305	hypothetical protein
Smon_0241	4	17	-3.844341	hypothetical protein
Smon_0242	4	15	-4.093084	hypothetical protein
Smon_0243	2	14	-3.575174	hypothetical protein

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Smon_0212	HTHTETR	40	2e-06	TetR bacterial regulatory protein HTH signature.
>HTHTETR#TetR bacterial regulatory protein HTH signature. Length = 215 Score = 39.6 bits (92), Expect = 2e-06 Identities = 12/64 (18%), Positives = 29/64 (45%) Query: 2 RRKIKAKDLIRNAFAKTLKVKPYYRITVKELTEETGVTRQIFYYYFKNMTELLKYYFEVE 61 + + + I + + + ++ E+ + GVTR Y++FK+ ++L +E+ Sbjct: 7 QEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSEIWELS 66 Query: 62 IQEI 65 I Sbjct: 67 ESNI 70
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Smon_0215	CARBMTKINASE	30	0.008	Bacterial carbamate kinase signature.
>CARBMTKINASE#Bacterial carbamate kinase signature. Length = 314 Score = 29.8 bits (67), Expect = 0.008 Identities = 45/239 (18%), Positives = 76/239 (31%), Gaps = 76/239 (31%) Query: 5 KRILLKLSGEALAGDKEFGFSDDI---LHSFAKQIKEIHDEGVELAIVIGG----GNIFR 57 KR+++ L G AL + G +++ + A+QI EI G E+ I G G++ Sbjct: 3 KRVVIALGGNALQQRGQKGSYEEMMDNVRKTARQIAEIIARGYEVVITHGNGPQVGSLLL 62 Query: 58 -------GKFGEEVGMDRSTGDTMG----MLATIMNGLALQNAIEK-IGGVSTRVLTAIN 105 MD + + G M+ + + +EK + + T+ + N Sbjct: 63 HMDAGQATYGIPAQPMDVAGAMSQGWIGYMIQQALKNELRKRGMEKKVVTIITQTIVDKN 122 Query: 106 MPQVAEP-------------------------------------------FIRRRAIRHL 122 P P + I+ L Sbjct: 123 DPAFQNPTKPVGPFYDEETAKRLAREKGWIVKEDSGRGWRRVVPSPDPKGHVEAETIKKL 182 Query: 123 -EKGRVVIFAGGTGNPYFTTDSG-------------GALRAIEIEANVLAKGTKVDGIY 167 E+G +VI +GG G P D G A E+ A++ T V+G Sbjct: 183 VERGVIVIASGGGGVPVILEDGEIKGVEAVIDKDLAGEKLAEEVNADIFMILTDVNGAA 241
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Smon_0217	SHAPEPROTEIN	339	e-118	Bacterial cell shape determinant MreB/Mbl protein s...
>SHAPEPROTEIN#Bacterial cell shape determinant MreB/Mbl protein signature. Length = 347 Score = 339 bits (870), Expect = e-118 Identities = 145/351 (41%), Positives = 222/351 (63%), Gaps = 13/351 (3%) Query: 2 IFNKIINFFRIKKQISIDLGTSNVLFYDKQAKKIVLNEPSVIVKDKK----TDRVVAVGR 57 + K F +SIDLGT+N L Y K + IVLNEPSV+ + V AVG Sbjct: 1 MLKKFRGMFS--NDLSIDLGTANTLIYVK-GQGIVLNEPSVVAIRQDRAGSPKSVAAVGH 57 Query: 58 EAREMLGKNPKSIEVIKPLKDGVISDIDLTRKMLSEFMRQVYGISPF--KPEVIICVPIE 115 +A++MLG+ P +I I+P+KDGVI+D +T KML F++QV+ S P V++CVP+ Sbjct: 58 DAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNSFMRPSPRVLVCVPVG 117 Query: 116 VTKVERRALFDALDDV--KRIFLIEEGRAAIMGAGINISNPNGHMVIDIGGGSTDVAILS 173 T+VERRA+ ++ + +FLIEE AA +GAG+ +S G MV+DIGGG+T+VA++S Sbjct: 118 ATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATGSMVVDIGGGTTEVAVIS 177 Query: 174 LDEIIVSKSIKIAGNKFDEDIVKYVKEKLFLNIGDRTAEKIKKELSTAIFLPEEENKKMT 233 L+ ++ S S++I G++FDE I+ YV+ IG+ TAE+IK E+ +A P +E +++ Sbjct: 178 LNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERIKHEIGSA--YPGDEVREIE 235 Query: 234 IKGLDINTKKPKELVITSNQVCEAIEDSLNNLVAAVKEVIGKCPPELASDILDNGIVLTG 293 ++G ++ P+ + SN++ EA+++ L +V+AV + +CPPELASDI + G+VLTG Sbjct: 236 VRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALEQCPPELASDISERGMVLTG 295 Query: 294 GGALISNLYKLIENEVKVNVHVPDKPLDSVAIGGSYAFDNKNLLNTLLVKE 344 GGAL+ NL +L+ E + V V + PL VA GG A + ++ L E Sbjct: 296 GGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALEMIDMHGGDLFSE 346
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Smon_0219	SYCDCHAPRONE	34	2e-04	Gram-negative bacterial type III secretion SycD cha...
>SYCDCHAPRONE#Gram-negative bacterial type III secretion SycD chaperone signature. Length = 168 Score = 33.7 bits (77), Expect = 2e-04 Identities = 20/142 (14%), Positives = 40/142 (28%), Gaps = 6/142 (4%) Query: 46 AYLENDKAIKLYEELSKYLPNDHEVEGYLGYLYYENSNLNEAEERLKNALYLSEKEPFLL 105 ++L+ I + E+S + Y++ +A + + L + Sbjct: 17 SFLKGGGTIAMLNEISSDTLEQLYSLAFN---QYQSGKYEDAHKVFQALCVLDHYDSRFF 73 Query: 106 FLLGNVYSRKGMLREAFDCYELAIFLDFDMYGAHIDFGRKYEHMGRHRRALKEFRAAYDI 165 LG G A Y +D G A A ++ Sbjct: 74 LGLGACRQAMGQYDLAIHSYSYGAIMDIKEPRFPFHAAECLLQKGELAEAESGLFLAQEL 133 Query: 166 ---DSRDEELLKKIEHVENRIK 184 + +EL ++ + IK Sbjct: 134 IADKTEFKELSTRVSSMLEAIK 155
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Smon_0222	adhesinb	266	1e-90	Adhesin B signature.
>adhesinb#Adhesin B signature. Length = 310 Score = 266 bits (681), Expect = 1e-90 Identities = 88/313 (28%), Positives = 162/313 (51%), Gaps = 13/313 (4%) Query: 1 MKNLKQILLALMLTVFAFSCGSKMGDAKSDEGKIKVTTTLNYYVNLLEEIGKDKVKVTGL 60 MK + ++L L+ V +C S+ ++ K+ V T + ++ + I DK+ + + Sbjct: 1 MKKCRFLVLLLLAFVGLAACSSQKSSTETGSSKLNVVATNSIIADITKNIAGDKINLHSI 60 Query: 61 MGEGEDPHLYVATAGDIEKLEKADLVVYGGLHLEGKMVEIFENL-------KDKAVLDLG 113 + G+DPH Y D++K +ADL+ Y G++LE F L ++K + Sbjct: 61 VPVGQDPHEYEPLPEDVKKTSQADLIFYNGINLETGGNAWFTKLVENAKKKENKDYYAVS 120 Query: 114 AQLDPSKLV-EEEKGVYDPHVWFNTEFWAVQATAVANKLSELDPANKEFYMNNLEVYLKE 172 +D L + EKG DPH W N E + A +A +LSE DPANKE Y NL+ Y+++ Sbjct: 121 EGVDVIYLEGQSEKGKEDPHAWLNLENGIIYAQNIAKRLSEKDPANKETYEKNLKAYVEK 180 Query: 173 LDMATKYVQDKINEIPENARVLITAHDAFGYFASQFGLEVKAIQGVSTDSEIGTKEINEL 232 L K ++K N IP ++++T+ F YF+ + + I ++T+ E +I L Sbjct: 181 LSALDKEAKEKFNNIPGEKKMIVTSEGCFKYFSKAYNVPSAYIWEINTEEEGTPDQIKTL 240 Query: 233 ADFIVANKIKAIFVESSVNHKSIESLQEAVQAKGFEVKIGGELYSDSMGDAKNNTETYIK 292 + + K+ ++FVESSV+ + ++++ +K + I ++++DS+ + ++Y Sbjct: 241 VEKLRKTKVPSLFVESSVDDRPMKTV-----SKDTNIPIYAKIFTDSVAEKGEEGDSYYS 295 Query: 293 TLKFNADTIANAL 305 +K+N + IA L Sbjct: 296 MMKYNLEKIAEGL 308
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Smon_0227	PF03895	50	1e-09	Serum resistance protein DsrA.
>PF03895#Serum resistance protein DsrA. Length = 79 Score = 50.2 bits (120), Expect = 1e-09 Identities = 23/74 (31%), Positives = 38/74 (51%), Gaps = 3/74 (4%) Query: 1838 GGVANAIAVASIPQINGKGH-NIGASYGYYEGHSAFALGL-SGINERGNVLYKANLSLNT 1895 G+AN A++ + Q NG G ++ A+ G Y +A A+G+ S I +R + Sbjct: 7 TGLANQSALSMLVQPNGVGKTSVSAAVGGYRDKTALAIGVGSRITDRFTAKAGVAFNTY- 65 Query: 1896 RGNVGIGAGIGYQF 1909 G + GA +GY+F Sbjct: 66 NGGMSYGASVGYEF 79
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Smon_0228	OMADHESIN	57	1e-10	Yersinia outer membrane adhesin signature.
>OMADHESIN#Yersinia outer membrane adhesin signature. Length = 455 Score = 56.8 bits (136), Expect = 1e-10 Identities = 61/241 (25%), Positives = 121/241 (50%), Gaps = 20/241 (8%) Query: 31 GDEANASAKYSIAIGRNSKSESEKSIAFGSEANSKGKYSIALGTEANSSGKTSIAIGRNS 90 G A+A +SIAIG + A + ++A+G + ++G S+AIG S Sbjct: 62 GLNASAKGIHSIAIG--------------ATAEAAKGAAVAVGAGSIATGVNSVAIGPLS 107 Query: 91 KSESEKSIAFGSGANSKGKYSIALGTEANSSGKSSIAIGRNSKSESEKSIAFGNGAN--S 148 K+ + ++ +G+ + ++ K +A+G A++S + +A+G NSK++++ S+A G+ ++ + Sbjct: 108 KALGDSAVTYGAASTAQ-KDGVAIGARASTS-DTGVAVGFNSKADAKNSVAIGHSSHVAA 165 Query: 149 NRKYAIALGSSSRAMGVDAISIGQKSISKNSNSIAIGTSATSNIENSVALGAESETTVAK 208 N Y+IA+G S+ +++SIG +S+++ +A GT T + N L E E T Sbjct: 166 NHGYSIAIGDRSKTDRENSVSIGHESLNRQLTHLAAGTKDTDAV-NVAQLKKEIEKTQEN 224 Query: 209 PTSEIVKPRFFLD-YKDFAGSNPYGVVSIGSKGKERQLQYVAAGQISKESTDAVNGSQLF 267 + + Y D S+ G+ + + K + A + +S D +N ++ Sbjct: 225 TNKRSAELLANANAYADNKSSSVLGIANNYTDSKSAETLENARKEAFAQSKDVLNMAKAH 284 Query: 268 S 268 S Sbjct: 285 S 285 Score = 50.7 bits (120), Expect = 1e-08 Identities = 53/200 (26%), Positives = 99/200 (49%), Gaps = 7/200 (3%) Query: 24 GEKSIAIGDEANASAKYSIAIGRNSKSESEKSIAFGSEANSKGKYSIALGTEANSSGKTS 83 G SIAIG A A+ ++A+G S + S+A G + + G ++ G A+++ K Sbjct: 69 GIHSIAIGATAEAAKGAAVAVGAGSIATGVNSVAIGPLSKALGDSAVTYGA-ASTAQKDG 127 Query: 84 IAIG-RNSKSESEKSIAFGSGANSKGKYSIALGTEANSSGKSSIAIGRNSKSESEKSIAF 142 +AIG R S S++ ++ F S A++K +I + ++ SIAIG SK++ E S++ Sbjct: 128 VAIGARASTSDTGVAVGFNSKADAKNSVAIGHSSHVAANHGYSIAIGDRSKTDRENSVSI 187 Query: 143 GNGANSNRKYAIALGSSSR-----AMGVDAISIGQKSISKNSNSIAIGTSATSNIENSVA 197 G+ + + + +A G+ A I Q++ +K S + +A ++ ++S Sbjct: 188 GHESLNRQLTHLAAGTKDTDAVNVAQLKKEIEKTQENTNKRSAELLANANAYADNKSSSV 247 Query: 198 LGAESETTVAKPTSEIVKPR 217 LG + T +K + R Sbjct: 248 LGIANNYTDSKSAETLENAR 267 Score = 32.9 bits (74), Expect = 0.003 Identities = 40/191 (20%), Positives = 73/191 (38%), Gaps = 17/191 (8%) Query: 19 ANAGTGEKSIAIGDEANASAKYSIAIGRNSKSESEKSIAFGSEANSKGKYSIALGTEANS 78 A A T + +A+G + A AK S+AIG +S + YSIA+G + + Sbjct: 132 ARASTSDTGVAVGFNSKADAKNSVAIGHSSHVAANHG------------YSIAIGDRSKT 179 Query: 79 SGKTSIAIGRNSKSESEKSIAFGSGAN-----SKGKYSIALGTEANSSGKSSIAIGRNSK 133 + S++IG S + +A G+ ++ K I E + + + N+ Sbjct: 180 DRENSVSIGHESLNRQLTHLAAGTKDTDAVNVAQLKKEIEKTQENTNKRSAELLANANAY 239 Query: 134 SESEKSIAFGNGANSNRKYAIALGSSSRAMGVDAISIGQKSISKNSNSIAIGTSATSNIE 193 ++++ S G N + ++R +SNS+A T T+ Sbjct: 240 ADNKSSSVLGIANNYTDSKSAETLENARKEAFAQSKDVLNMAKAHSNSVARTTLETAEEH 299 Query: 194 NSVALGAESET 204 + ET Sbjct: 300 ANSVARTTLET 310
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Smon_0237	OMPADOMAIN	105	2e-27	OMPA domain signature.
>OMPADOMAIN#OMPA domain signature. Length = 346 Score = 105 bits (264), Expect = 2e-27 Identities = 49/148 (33%), Positives = 75/148 (50%), Gaps = 8/148 (5%) Query: 342 EVVKEVEVPVHIKPQI--KKIELSADALFKFDKYKLEDMLEKGKMEIQELVKKLSTDYVR 399 E V P++ K L +D LF F+K L+ +G+ + +L +LS + Sbjct: 195 EAAPVVAPAPAPAPEVQTKHFTLKSDVLFNFNKATLK---PEGQAALDQLYSQLSNLDPK 251 Query: 400 LDRIDIIGHTDRLGSDSYNLALGLRRAQTVRSYLQELGVTTP-ITVASKGKRDPKV--KC 456 + ++G+TDR+GSD+YN L RRAQ+V YL G+ I+ G+ +P C Sbjct: 252 DGSVVVLGYTDRIGSDAYNQGLSERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTC 311 Query: 457 PGTKATAKLKQCLLPNRRVEINLTGLEV 484 K A L CL P+RRVEI + G++ Sbjct: 312 DNVKQRAALIDCLAPDRRVEIEVKGIKD 339
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Smon_0238	PF03309	194	1e-63	Bvg accessory factor
>PF03309#Bvg accessory factor Length = 271 Score = 194 bits (495), Expect = 1e-63 Identities = 62/246 (25%), Positives = 117/246 (47%), Gaps = 16/246 (6%) Query: 1 MILGFDIGNTHICPIIYDNN---GKILEKFRIPSKTNLTEDTLYATLKTLCDFKKIDLSD 57 M+L D+ NTH + + K+++++RI ++ +T D L T+ L D Sbjct: 1 MLLAIDVRNTHTVVGLISGSGDHAKVVQQWRIRTEPEVTADELALTIDGLI---GDDAER 57 Query: 58 VKDVVYSSVVPHLNNVFDYLAKKYFNCEPYVLNINNIDENLLTFNANTERNLGADRIA-T 116 + S VP + + + ++Y+ P+VL + + + + +GADRI Sbjct: 58 LTGASGLSTVPSVLHEVRVMLEQYWPNVPHVLIEPGVRTGI-PLLVDNPKEVGADRIVNC 116 Query: 117 ILAMKKYMSNKKCIIIDFGTATTFEVI-KDNKYLGGAILPGIDLSINALFQNTAKLPKVT 175 + A KY + I++DFG++ +V+ ++LGGAI PG+ +S +A +A L +V Sbjct: 117 LAAYHKYGTA--AIVVDFGSSICVDVVSAKGEFLGGAIAPGVQVSSDAAAARSAALRRVE 174 Query: 176 FEKPNEVLGNTTVTQINIGIYYSNIGAIKELINQYKNIYP-----DAYVISTGGQGKIIT 230 +P V+G TV + G + G + L+N+ ++ D V++TG ++ Sbjct: 175 LTRPRSVIGKNTVECMQAGAVFGFAGLVDGLVNRIRDDVDGFSGADVAVVATGHTAPLVL 234 Query: 231 EDLKDF 236 DL+ Sbjct: 235 PDLRTV 240
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Smon_0239	BCTERIALGSPG	50	7e-11	Bacterial general secretion pathway protein G signa...
>BCTERIALGSPG#Bacterial general secretion pathway protein G signature. Length = 145 Score = 50.3 bits (120), Expect = 7e-11 Identities = 25/58 (43%), Positives = 36/58 (62%) Query: 11 KKNRGFTLIEIITVIAIIGILASISVPKISKYIDRANETKIFSAVSELNNLYILMNLD 68 K RGFTL+EI+ VI IIG+LAS+ VP + ++A++ K S + L N + LD Sbjct: 5 DKQRGFTLLEIMVVIVIIGVLASLVVPNLMGNKEKADKQKAVSDIVALENALDMYKLD 62
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Smon_0240	PREPILNPTASE	33	3e-04	Type IV prepilin cysteine protease (C20) family sig...
>PREPILNPTASE#Type IV prepilin cysteine protease (C20) family signature. Length = 290 Score = 32.9 bits (75), Expect = 3e-04 Identities = 28/136 (20%), Positives = 53/136 (38%), Gaps = 15/136 (11%) Query: 6 YLMLLYISYVDFVEGYIYDR-DLVILFIFLYFSTTSGIYSSYVGMGIFSLPFFILWILES 64 +L+ ++++D + + D+ L +L+ L F+ G S + + +LW L Sbjct: 141 TWVLVALTFIDLDKMLLPDQLTLPLLWGGLLFNLLGGFVSLGDAVIGAMAGYLVLWSLYW 200 Query: 65 YFNF----EIIGMGDIKLMLIFGMYFGIKDMHFIFTFYEIMYFSSLIYAIILR------- 113 F E +G GD KL+ G + G + + + + I L Sbjct: 201 AFKLLTGKEGMGYGDFKLLAALGAWLGWQALPIVLLLSSL---VGAFMGIGLILLRNHHQ 257 Query: 114 KKYVPFAPAMCFSFII 129 K +PF P + + I Sbjct: 258 SKPIPFGPYLAIAGWI 273

Smon_0601

Smon_0619

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
Smon_0601	0	9	-4.461323	*hypothetical protein
Smon_0602	-1	9	-3.668017	tRNA (guanine-N(7)-)-methyltransferase
Smon_0603	1	9	-4.967787	hypothetical protein
Smon_0604	5	10	-6.259394	hypothetical protein
Smon_0605	5	9	-7.475396	hypothetical protein
Smon_0606	4	8	-7.193414	FHA domain containing protein
Smon_0607	1	9	-5.161683	hypothetical protein
Smon_0608	0	9	-4.632775	hypothetical protein
Smon_0609	-1	10	-3.451860	Mn2+dependent serine/threonine protein kinase
Smon_0610	-1	10	-1.909700	hypothetical protein
Smon_0611	-1	11	0.056273	rare lipoprotein A
Smon_0612	-3	12	0.016984	Amidase
Smon_0613	-1	12	-0.662263	peptidase S9 prolyl oligopeptidase active site
Smon_0614	0	11	-0.549260	putative PTS IIA-like nitrogen-regulatory
Smon_0615	0	11	-0.491953	peptide chain release factor 1
Smon_0616	0	12	-1.836582	modification methylase, HemK family
Smon_0617	2	13	-2.259374	S-adenosylmethionine/tRNA-ribosyltransferase-iso
Smon_0618	1	10	-1.116283	methyltransferase
Smon_0619	2	10	-1.830371	Exonuclease VII small subunit

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Smon_0605	PF03944	30	0.011	delta endotoxin
>PF03944#delta endotoxin Length = 633 Score = 29.6 bits (66), Expect = 0.011 Identities = 16/58 (27%), Positives = 32/58 (55%), Gaps = 1/58 (1%) Query: 130 GATLKNYDYELKKFSKEYNSLNINEVRYSKTGINPKLLKIWS-KKMMALKLDESMDLW 186 ATL+ Y LK ++++Y++ IN + + G+N +L + + M L + E + +W Sbjct: 202 AATLRTYRDYLKNYTRDYSNYCINTYQSAFKGLNTRLHDMLEFRTYMFLNVFEYVSIW 259
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Smon_0609	TCRTETOQM	28	0.037	Tetracycline resistance protein TetO/TetQ/TetM family ...
>TCRTETOQM#Tetracycline resistance protein TetO/TetQ/TetM family signature. Length = 639 Score = 27.9 bits (62), Expect = 0.037 Identities = 17/74 (22%), Positives = 31/74 (41%), Gaps = 6/74 (8%) Query: 45 KILKVKITKNNKEIFYERIVGKTLKEMNILNYSLKERLKIFNEIIVAVEKIHNLGLIHND 104 K+ K++ ++ + + Y R+ L + + S KE++KI N L D Sbjct: 252 KVFKIEYSEKRQRLAYIRLYSGVLHLRDSVRISEKEKIKITEMYT-----SINGELCKID 306 Query: 105 -INLGNIIINENNV 117 G I+I +N Sbjct: 307 KAYSGEIVILQNEF 320

Smon_0643

Smon_0665

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
Smon_0643	4	31	5.207677	RNA methyltransferase, TrmA family
Smon_0644	8	42	8.200164	hypothetical protein
Smon_0645	6	43	8.288771	hypothetical protein
Smon_0646	7	47	8.844392	replication initiator A domain protein
Smon_0649	7	48	9.083000	hypothetical protein
Smon_0650	7	41	8.078286	hypothetical protein
Smon_0652	5	36	6.257461	KilA domain protein
Smon_0654	5	34	6.018898	RNA-directed DNA polymerase
Smon_0655	5	30	5.101840	hypothetical protein
Smon_0656	4	25	4.315800	transcriptional regulator, XRE family
Smon_0657	4	25	3.933387	N-6 DNA methylase
Smon_0658	4	29	4.388075	restriction modification system DNA specificity
Smon_0659	5	30	4.423295	protein of unknown function DUF450
Smon_0660	5	31	4.377918	hypothetical protein
Smon_0661	5	34	4.810777	conserved hypothetical protein
Smon_0662	5	34	4.395372	DEAD/DEAH box helicase domain protein
Smon_0663	5	33	3.763270	hypothetical protein
Smon_0664	5	24	1.303288	RNA-directed DNA polymerase
Smon_0665	2	17	-0.274535	relaxase

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Smon_0650	GPOSANCHOR	30	0.017	Gram-positive coccus surface protein anchor signature.
>GPOSANCHOR#Gram-positive coccus surface protein anchor signature. Length = 539 Score = 30.4 bits (68), Expect = 0.017 Identities = 12/133 (9%), Positives = 45/133 (33%) Query: 112 RKVAKAELKDEETKTEEKKPQQDKSTQTGDKKTKDKSTQTELSKEDISKMEKQAKELKGE 171 L+ E+ E ++ + +K+ + + S + + + + + + + +L+ Sbjct: 174 DSAKIKTLEAEKAALEARQAELEKALEGAMNFSTADSAKIKTLEAEKAALAARKADLEKA 233 Query: 172 LDKLNGEIKDKDKLNDKQKEKIKALEDKIDSLKEKMKKDKGNKDLSEDMKKEIDKLTEKV 231 L+ + + ALE + L++ ++ K ++ + Sbjct: 234 LEGAMNFSTADSAKIKTLEAEKAALEARQAELEKALEGAMNFSTADSAKIKTLEAEKAAL 293 Query: 232 KELEKKATETNKA 244 + + ++ Sbjct: 294 EAEKADLEHQSQV 306

Smon_0680

Smon_0689

Genomic Island

LocusTag	DNBias	CDNBias	%GCBias	Product
Smon_0680	0	9	-3.120359	Nicotinamide phosphoribosyltransferase
Smon_0681	0	8	-4.182502	Chloride channel core
Smon_0682	3	8	-5.058475	hypothetical protein
Smon_0683	5	9	-4.288281	putative small multi-drug export
Smon_0684	6	7	-4.687185	hypothetical protein
Smon_0685	5	7	-4.404117	hypothetical protein
Smon_0686	5	8	-4.572202	hypothetical protein
Smon_0687	2	7	-2.904424	hypothetical protein
Smon_0688	2	7	-2.889517	hypothetical protein
Smon_0689	1	7	-3.380883	hypothetical protein

Smon_0710

Smon_0728

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
Smon_0710	1	15	-3.287331	SsrA-binding protein
Smon_0711	1	15	-3.147200	VacB and RNase II family 3'-5' exoribonuclease
Smon_0712	2	15	-2.016954	metal dependent phosphohydrolase
Smon_0713	0	15	-1.325256	conserved hypothetical protein
Smon_0714	1	14	0.457668	N-acetylmuramoyl-L-alanine amidase
Smon_0715	2	14	1.188728	preprotein translocase, YajC subunit
Smon_0716	4	13	1.451297	DNA-(apurinic or apyrimidinic site) lyase
Smon_0717	2	11	0.943267	protein of unknown function DUF395 YeeE/YedE
Smon_0718	-1	10	0.691322	SirA family protein
Smon_0719	0	9	0.561588	protein of unknown function DUF395 YeeE/YedE
Smon_0720	1	9	0.465990	FAD-dependent pyridine nucleotide-disulphide
Smon_0721	2	10	-0.277842	hypothetical protein
Smon_0722	2	8	-0.973988	transcriptional regulator, LysR family
Smon_0723	2	8	-0.565969	ABC transporter related protein
Smon_0724	0	10	-0.655277	NUDIX hydrolase
Smon_0725	0	10	-1.120829	magnesium transporter
Smon_0726	0	13	-1.398085	hypothetical protein
Smon_0727	2	12	-1.054787	protein of unknown function DUF45
Smon_0728	2	12	0.098342	band 7 protein

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Smon_0718	PF01206	67	8e-19	SirA family protein
>PF01206#SirA family protein Length = 76 Score = 66.7 bits (163), Expect = 8e-19 Identities = 15/71 (21%), Positives = 40/71 (56%) Query: 4 EFTLDCLGEACPVPLIRTQGKMEELEIGDVLVVSIDHSCAMKNIPEWARKVGHNVEIEEI 63 + +LD G CP+P+++ + + + G+VL V ++K+ ++++ GH + ++ Sbjct: 5 DQSLDATGLNCPLPILKAKKTLATMNAGEVLYVMATDPGSVKDFESFSKQTGHELLEQKE 64 Query: 64 DDGEWELIIEK 74 +DG + +++ Sbjct: 65 EDGTYHFRLKR 75
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Smon_0721	PF01206	41	1e-08	SirA family protein
>PF01206#SirA family protein Length = 76 Score = 40.9 bits (96), Expect = 1e-08 Identities = 14/64 (21%), Positives = 26/64 (40%) Query: 7 GEICPIPFLKFEKIFKEINTGENFTIIVDHSCAKVKIENFCKNKNIRFKIFEPINGIWEI 66 G CP+P LK +K +N GE ++ + E+F K + +G + Sbjct: 12 GLNCPLPILKAKKTLATMNAGEVLYVMATDPGSVKDFESFSKQTGHELLEQKEEDGTYHF 71 Query: 67 TVWK 70 + + Sbjct: 72 RLKR 75
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Smon_0725	FLGMOTORFLIG	31	0.009	Flagellar motor switch protein FliG signature.
>FLGMOTORFLIG#Flagellar motor switch protein FliG signature. Length = 344 Score = 30.9 bits (70), Expect = 0.009 Identities = 50/236 (21%), Positives = 90/236 (38%), Gaps = 12/236 (5%) Query: 7 MEKLTQEEIELLKQEIKS--RLDHEEEEEYFEEDIDTEEIAEDLQNLESEKEIEEFVEEH 64 + L+QEEIE L EI + E ++ E + A++ E +E+ Sbjct: 37 FKYLSQEEIESLTFEIAKLETITSELKDNVLLEFKELMM-AQEFIQKGGIDYARELLEKS 95 Query: 65 HSVDIADSFYEIDDDTELLRVFNLFS--DDVKI-ELFEQSDEKLQVRILNLLELDKAIDI 121 A R F D I +Q + IL+ L+ KA I Sbjct: 96 LGTQKAVDIINNLGSALQSRPFEFVRRADPANILNFIQQEHPQTIALILSYLDPQKASFI 155 Query: 122 LTYLPPDDVADILGTLELRK--SKEILDNMKRSDANKIRLLLGYEDDTAGGI-MTTQYIA 178 L+ LP + ++ + L S E++ ++R K+ L + +AGG+ + I Sbjct: 156 LSSLPTEVQTNVARRIALMDRTSPEVVREVERVLEKKLASLSSEDYTSAGGVDNVVEIIN 215 Query: 179 FKKNLKIKDIMEKIKIIGPK--TEYIETIFVLDEEARLFGEADLRDILISSDDTTL 232 K I+E ++ P+ E + +FV ++ L + ++ +L D L Sbjct: 216 MADRKTEKFIIESLEEEDPELAEEIKKKMFVFEDIVLL-DDRSIQRVLREIDGQEL 270

Smon_0739

Smon_0748

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
Smon_0739	0	12	-3.430817	replicative DNA helicase
Smon_0740	-1	10	-4.206541	ribosomal protein L9
Smon_0741	1	10	-5.558322	DNA polymerase III, subunits gamma and tau
Smon_0742	2	12	-6.094520	hypothetical protein
Smon_0743	1	11	-5.401896	Shikimate 5-dehydrogenase-like protein
Smon_0744	2	11	-4.707311	pseudouridine synthase
Smon_0745	1	11	-3.467623	hypothetical protein
Smon_0748	0	10	-3.424509	protein of unknown function DUF214

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Smon_0744	FLGMOTORFLIM	29	0.020	Flagellar motor switch protein FliM signature.
>FLGMOTORFLIM#Flagellar motor switch protein FliM signature. Length = 344 Score = 28.7 bits (64), Expect = 0.020 Identities = 21/93 (22%), Positives = 38/93 (40%), Gaps = 3/93 (3%) Query: 140 ISSDDISNIEKGIDIGEGIITRLSKIIKVDDNKLYIIITEGKFHQVK-RMFKAVNNEVKY 198 +S D+I + I G+ I I LY KF + + R ++ Sbjct: 5 LSQDEIDQLLTAISSGDASIEDARPISDTRKITLYDFRRPDKFSKEQMRTLSLMHETFAR 64 Query: 199 LKRISMGSLILDE-KLNLGEYRELTYEE-LRSL 229 L S+ + + +++ +LTYEE +RS+ Sbjct: 65 LTTTSLSAQLRSMVHVHVASVDQLTYEEFIRSI 97
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Smon_0748	GPOSANCHOR	67	2e-13	Gram-positive coccus surface protein anchor signature.
>GPOSANCHOR#Gram-positive coccus surface protein anchor signature. Length = 539 Score = 67.0 bits (163), Expect = 2e-13 Identities = 65/355 (18%), Positives = 124/355 (34%), Gaps = 41/355 (11%) Query: 186 ENKEYTFANIKLKTNFNTYEKEYNDYIFEKKIDIKNKLNKNSKIKLDEFLSEKYDEINKG 245 +N + +F N LK + + +E ++ + + + K+ K SKI+ E ++ +G Sbjct: 72 KNSDLSFNNKALKDHNDELTEELSNAKEKLRKNDKSLSEKASKIQELEARKADLEKALEG 131 Query: 246 KNKIEN---SKNEIMKNENKIIDAKNKILSAE--------KNLISKINEFQNANDKFYSS 294 +K + ++ E + A+ L +KI + + Sbjct: 132 AMNFSTADSAKIKTLEAEKAALAARKADLEKALEGAMNFSTADSAKIKTLEAEKAALEAR 191 Query: 295 EVEINNQKVKLLEKQYNLKENLSKLEEGLKELEINKNKINNGLNEIEKGFNDIDENQKKI 354 + E+ + + LE L K + +E N + KI Sbjct: 192 QAELEKALEGAMNFSTADSAKIKTLEAEKAALAARKADLEK---ALEGAMNFSTADSAKI 248 Query: 355 DVSKEEILKNEKYINPLKPSIFVSKKKIEDGKSKIEKAIKEIELGEN---RLKDELKNLE 411 + E E L+ ++ + +KI+ E E L+ + + L Sbjct: 249 KTLEAEKAALEARQAELEKALEGAMNFSTADSAKIKTLEAEKAALEAEKADLEHQSQVLN 308 Query: 412 NKKIELINNLSFVENKKNELE------ENKKKINDG-LEQIKLGLDKLEEGNKKLIEEKN 464 + L +L K +LE E + KI++ + ++ LD E K+L E Sbjct: 309 ANRQSLRRDLDASREAKKQLEAEHQKLEEQNKISEASRQSLRRDLDASREAKKQLEAEHQ 368 Query: 465 KFNEEYE-----------------ENLSKIIKAKEEIKSRKKEIEKAAKKFEEEK 502 K E+ + E ++ KA EE S+ +EK K+ EE K Sbjct: 369 KLEEQNKISEASRQSLRRDLDASREAKKQVEKALEEANSKLAALEKLNKELEESK 423

Smon_0758

Smon_0771

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
Smon_0758	-3	13	-3.217789	hypothetical protein
Smon_0759	-2	10	-2.144825	maf protein
Smon_0760	-3	10	-1.493725	inosine guanosine and xanthosine phosphorylase
Smon_0761	-2	12	-1.789119	integral membrane protein MviN
Smon_0762	0	11	-0.766429	chromosome segregation and condensation protein
Smon_0763	-1	10	-0.993729	riboflavin biosynthesis protein RibF
Smon_0764	0	9	-0.731527	ATP-dependent protease La
Smon_0765	3	9	-0.228736	ATP-dependent Clp protease, ATP-binding subunit
Smon_0766	2	8	-0.632428	ATP-dependent Clp protease, proteolytic subunit
Smon_0767	3	9	-0.754574	trigger factor
Smon_0768	2	8	-1.124818	phosphoesterase RecJ domain protein
Smon_0769	4	8	0.573709	ribosome-binding factor A
Smon_0770	3	9	0.623510	translation initiation factor IF-2
Smon_0771	2	10	0.166354	hypothetical protein

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Smon_0765	HTHFIS	29	0.046	FIS bacterial regulatory protein HTH signature.
>HTHFIS#FIS bacterial regulatory protein HTH signature. Length = 484 Score = 28.6 bits (64), Expect = 0.046 Identities = 7/21 (33%), Positives = 14/21 (66%) Query: 106 KSNILLIGPTGSGKTLLAQTL 126 +++ G +G+GK L+A+ L Sbjct: 160 DLTLMITGESGTGKELVARAL 180
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Smon_0770	TCRTETOQM	91	8e-21	Tetracycline resistance protein TetO/TetQ/TetM family ...
>TCRTETOQM#Tetracycline resistance protein TetO/TetQ/TetM family signature. Length = 639 Score = 90.7 bits (225), Expect = 8e-21 Identities = 44/139 (31%), Positives = 68/139 (48%), Gaps = 18/139 (12%) Query: 402 ITIMGHVDHGKTSLLDALRHTNVIDGEAG------------------GITQRIGAYQVEW 443 I ++ HVD GKT+L ++L + + E G GIT + G +W Sbjct: 6 IGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTDNTLLERQRGITIQTGITSFQW 65 Query: 444 NGQKITFIDTPGHEAFTEMRVRGANITDISILIVAADDGVKPQTIEAISHAKEANVPIIV 503 K+ IDTPGH F R ++ D +IL+++A DGV+ QT ++ +P I Sbjct: 66 ENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAKDGVQAQTRILFHALRKMGIPTIF 125 Query: 504 AINKIDKPGANPMKVKQEL 522 INKID+ G + V Q++ Sbjct: 126 FINKIDQNGIDLSTVYQDI 144

Smon_0822

Smon_0834

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
Smon_0822	2	9	-2.428413	outer membrane autotransporter barrel domain
Smon_0823	0	11	-2.482442	outer membrane autotransporter barrel domain
Smon_0824	-1	18	-2.528643	hypothetical protein
Smon_0825	-1	10	-2.319536	glycosyl transferase group 1
Smon_0826	-1	9	-1.758179	hypothetical protein
Smon_0827	-2	10	-1.838308	hypothetical protein
Smon_0828	-3	10	-2.376210	Adenosylhomocysteine nucleosidase
Smon_0829	-2	9	-2.765822	Ferritin Dps family protein
Smon_0830	0	8	-3.099937	sigma 54 modulation protein/ribosomal protein
Smon_0831	-1	9	-3.298241	ABC transporter related protein
Smon_0832	0	8	-4.352347	protein of unknown function DUF214
Smon_0833	2	10	-5.303311	hypothetical protein
Smon_0834	1	10	-4.588933	Septum formation initiator

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Smon_0829	HELNAPAPROT	138	9e-45	Helicobacter neutrophil-activating protein A family ...
>HELNAPAPROT#Helicobacter neutrophil-activating protein A family signature. Length = 153 Score = 138 bits (348), Expect = 9e-45 Identities = 48/141 (34%), Positives = 81/141 (57%), Gaps = 1/141 (0%) Query: 4 TIDALNIYLADLNVFYRKVQNYHWNVVGQGFFTVHAKLEEIYDAVNEKIDIIAERVLSIG 63 ++LN L++ + Y K+ +HW V G FFT+H K EE+YD E +D IAER+L+IG Sbjct: 13 VENSLNTQLSNWFLLYSKLHRFHWYVKGPHFFTLHEKFEELYDHAAETVDTIAERLLAIG 72 Query: 64 GRPYGSMKKYLEVTTITEAKDEDITVKELLNVLIVDVENLLGQVKNLKNITDEEGDFGTS 123 G+P ++K+Y E +IT+ + + E++ L+ D + + + K + + +E D T+ Sbjct: 73 GQPVATVKEYTEHASITDG-GNETSASEMVQALVNDYKQISSESKFVIGLAEENQDNATA 131 Query: 124 AELDNHIAEYEKLLWMFKAYI 144 I E EK +WM +Y+ Sbjct: 132 DLFVGLIEEVEKQVWMLSSYL 152
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Smon_0831	PF05272	30	0.006	Virulence-associated E family protein
>PF05272#Virulence-associated E family protein Length = 892 Score = 30.4 bits (68), Expect = 0.006 Identities = 14/46 (30%), Positives = 21/46 (45%), Gaps = 5/46 (10%) Query: 36 IQGKSGSGKTTLLNILGLLDDVTEGDILI-----DGEKINNRDIIE 76 ++G G GK+TL+N L LD ++ I E+I E Sbjct: 601 LEGTGGIGKSTLINTLVGLDFFSDTHFDIGTGKDSYEQIAGIVAYE 646
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Smon_0834	60KDINNERMP	28	0.007	60kDa inner membrane protein signature.
>60KDINNERMP#60kDa inner membrane protein signature. Length = 548 Score = 28.0 bits (62), Expect = 0.007 Identities = 11/58 (18%), Positives = 28/58 (48%), Gaps = 6/58 (10%) Query: 6 YTLAIILLFF---AIFSALIFRTYNNVKKSREINIKLIESNKKYEKLKEEKEKLQKEL 60 + +II++ F I L Y ++ K R + K+ ++ ++K+++ +E+ Sbjct: 354 WGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQAMRERL---GDDKQRISQEM 408

Smon_0859

Smon_0908

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
Smon_0859	0	25	-3.593940	hypothetical protein
Smon_0860	-1	11	-3.081704	hypothetical protein
Smon_0862	0	10	-3.274714	hypothetical protein
Smon_0863	0	9	-3.509185	transcriptional regulator, XRE family
Smon_0864	1	10	-3.840428	hypothetical protein
Smon_0865	2	9	-3.439088	integrase family protein
Smon_0866	1	10	-3.565825	DNA topoisomerase type IA central domain
Smon_0867	4	9	-4.356012	hypothetical protein
Smon_0868	0	10	-3.510235	hypothetical protein
Smon_0869	0	10	-2.712996	cyclic nucleotide-binding domain containing
Smon_0870	1	9	-2.387758	hypothetical protein
Smon_0871	1	12	-2.104356	NLP/P60 protein
Smon_0872	3	12	-1.881929	hypothetical protein
Smon_0873	5	14	-2.295592	OmpA/MotB domain protein
Smon_0874	4	14	-2.124940	hypothetical protein
Smon_0875	3	13	-2.573658	hypothetical protein
Smon_0876	4	16	-2.547375	hypothetical protein
Smon_0877	-2	11	-0.370510	hypothetical protein
Smon_0878	-2	10	0.253905	hypothetical protein
Smon_0879	0	8	-0.331825	hypothetical protein
Smon_0880	-1	9	-0.295588	virulence-associated protein D (VapD) conserved
Smon_0881	-2	9	-0.978690	hypothetical protein
Smon_0882	-2	10	-1.349854	hypothetical protein
Smon_0883	-2	11	-2.680725	hypothetical protein
Smon_0884	-2	12	-3.815570	CagE TrbE VirB component of type IV transporter
Smon_0885	1	14	-5.429538	hypothetical protein
Smon_0886	1	16	-5.503674	hypothetical protein
Smon_0887	1	15	-4.076827	hypothetical protein
Smon_0888	2	15	-4.170172	YadA domain protein
Smon_0889	0	11	-1.647582	hypothetical protein
Smon_0890	1	11	-1.609343	hypothetical protein
Smon_0891	0	11	-1.361037	hypothetical protein
Smon_0892	1	10	-1.891372	type II secretion system protein E
Smon_0893	1	11	-2.461527	hypothetical protein
Smon_0894	0	10	-1.962811	TRAG family protein
Smon_0895	3	13	-4.648460	conjugation TrbI family protein
Smon_0896	4	14	-4.760985	Conjugal transfer protein TrbG/VirB9/CagX
Smon_0897	5	13	-5.418061	hypothetical protein
Smon_0898	4	12	-5.098267	Type IV secretory pathway TrbF protein-like
Smon_0899	4	13	-5.382402	hypothetical protein
Smon_0900	6	15	-6.386855	MobA/MobL protein
Smon_0902	5	17	-6.507127	prophage antirepressor
Smon_0903	6	17	-8.212157	hypothetical protein
Smon_0904	5	17	-6.030962	hypothetical protein
Smon_0905	6	20	-4.682130	hypothetical protein
Smon_0906	7	20	-4.910732	hypothetical protein
Smon_0907	4	16	-3.680980	replication initiator A domain protein
Smon_0908	4	16	-2.382297	hypothetical protein

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Smon_0868	SECFTRNLCASE	28	0.002	Bacterial translocase SecF protein signature.
>SECFTRNLCASE#Bacterial translocase SecF protein signature. Length = 333 Score = 27.9 bits (62), Expect = 0.002 Identities = 11/45 (24%), Positives = 21/45 (46%), Gaps = 2/45 (4%) Query: 8 YSYQKCIDIYDRIIENEKMKEYDKGILKKNINNKIENEISDNWNT 52 YS + ++DR+ EN + +Y L+ +N + +S T Sbjct: 218 YSINDTVVVFDRLREN--LIKYKTMPLRDVMNLSVNETLSRTVMT 260
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Smon_0873	OMPADOMAIN	73	1e-16	OMPA domain signature.
>OMPADOMAIN#OMPA domain signature. Length = 346 Score = 73.0 bits (179), Expect = 1e-16 Identities = 40/113 (35%), Positives = 52/113 (46%), Gaps = 16/113 (14%) Query: 218 FNSNKFILNKKQIDVIDALVPNLQN-----REIIVIGYTDTDGNDKYNLELGLNRANSVK 272 FN NK L + +D L L N ++V+GYTD G+D YN L RA SV Sbjct: 223 FNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLSERRAQSVV 282 Query: 273 VYLENKGIKVNQIRTVGFNEL--ITNNNTLENKSL---------NRRVELIVK 314 YL +KGI ++I G E +T N K +RRVE+ VK Sbjct: 283 DYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEVK 335
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Smon_0880	PF04605	106	1e-33	Virulence-associated protein D (VapD)
>PF04605#Virulence-associated protein D (VapD) Length = 125 Score = 106 bits (267), Expect = 1e-33 Identities = 25/92 (27%), Positives = 43/92 (46%), Gaps = 3/92 (3%) Query: 3 AIAFDLRVDDLKKYYGEPYNKAYDEIRQELELLGFEWTQGSVYMSTTTQNNLTYVYKAIN 62 AI FDL L+KY+ + + Y I++ + GFE Q S Y S N V + +N Sbjct: 7 AINFDLSTKSLEKYF-KDTREPYSLIKKFMLENGFEHRQYSGYTSKEPINERR-VIRIVN 64 Query: 63 KLS-TIEWFKKSVRDIRAFKVEDWSDFTEIVK 93 KL+ W + V++ ++ + E ++ Sbjct: 65 KLTKKFTWLGECVKEFDITEIGEQYSLKETIQ 96

Smon_0922

Smon_0936

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
Smon_0922	2	10	-2.212334	protein of unknown function DUF187
Smon_0923	3	10	-3.110580	O-methyltransferase family 3
Smon_0924	3	12	-2.970075	ABC transporter related protein
Smon_0925	5	11	-2.468838	ABC transporter related protein
Smon_0926	3	11	-2.219295	binding-protein-dependent transport systems
Smon_0927	3	11	-2.461154	binding-protein-dependent transport systems
Smon_0928	0	10	-2.215017	cold-shock DNA-binding domain protein
Smon_0929	-2	11	-2.279413	pseudouridine synthase
Smon_0930	-1	10	-1.772801	rod shape-determining protein RodA
Smon_0931	0	9	-0.767729	peptidase M16 domain protein
Smon_0932	0	10	-0.796641	Colicin V production protein
Smon_0933	0	9	-0.163074	alanine racemase
Smon_0934	5	8	-1.135370	cytidylate kinase
Smon_0935	3	8	-0.784282	metallophosphoesterase
Smon_0936	3	8	-0.563224	RNA binding metal dependent phosphohydrolase

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Smon_0925	HTHFIS	29	0.012	FIS bacterial regulatory protein HTH signature.
>HTHFIS#FIS bacterial regulatory protein HTH signature. Length = 484 Score = 29.4 bits (66), Expect = 0.012 Identities = 8/16 (50%), Positives = 12/16 (75%) Query: 34 LIGSSGSGKSQIARAI 49 + G SG+GK +ARA+ Sbjct: 165 ITGESGTGKELVARAL 180
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Smon_0933	ALARACEMASE	278	4e-94	Alanine racemase signature.
>ALARACEMASE#Alanine racemase signature. Length = 356 Score = 278 bits (712), Expect = 4e-94 Identities = 88/355 (24%), Positives = 168/355 (47%), Gaps = 24/355 (6%) Query: 3 VYAIIDLDAFKKNLDKILEKIPASKVMAIVKANAYGHGSIKIVESAIEKGINFFGVARIE 62 + A +DL A K+NL + + ++V ++VKANAYGHG +I + + F + +E Sbjct: 5 IQASLDLQALKQNLSIVRQAATHARVWSVVKANAYGHGIERIWSAI--GATDGFALLNLE 62 Query: 63 EAEEILNIFPKVDVLVLSPIMK-SDIENAVSKGIHLTISSFEDIEYILENKING--NFHY 119 EA + K +L+L D+E + + S ++ + ++ + + Sbjct: 63 EAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHSNWQLKALQNARLKAPLDIYL 122 Query: 120 ALDTGMGRIGFMEDEIY------RAFSMLKPIGIYSHLSSADNDNEYTNMQIEKFNRIVN 173 +++GM R+GF D + RA + + + + SH + A++ + + + + + Sbjct: 123 KVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHFAEAEHP-DGISGAMARIEQAAE 181 Query: 174 DLDVKYKHILNSFGSINNYE-DYDLYRLGIIMYG----AEMTDI----FKPVMTFKARVN 224 L+ + + + NS ++ + E +D R GII+YG + DI +PVMT + + Sbjct: 182 GLECR-RSLSNSAATLWHPEAHFDWVRPGIILYGASPSGQWRDIANTGLRPVMTLSSEII 240 Query: 225 HIKVLEKDTYIGYSKTYLASCGEKIATISCGYADGMLRSMSNRSKVYFNGKYYPIVGNIC 284 ++ L+ +GY Y A ++I ++ GYADG R + V +G VG + Sbjct: 241 GVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGYPRHAPTGTPVLVDGVRTMTVGTVS 300 Query: 285 MDQFMIKV--DDDIKVNDYVEIFGNNILVGDLAKELNTISYELLCAISHRVKRIY 337 MD + + + VE++G I + D+A T+ YEL+CA++ RV + Sbjct: 301 MDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAAAAGTVGYELMCALALRVPVVT 355
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Smon_0936	GPOSANCHOR	31	0.014	Gram-positive coccus surface protein anchor signature.
>GPOSANCHOR#Gram-positive coccus surface protein anchor signature. Length = 539 Score = 30.8 bits (69), Expect = 0.014 Identities = 19/99 (19%), Positives = 34/99 (34%) Query: 57 EITKKDVEREIESFRKEETLKVKEELLAQKKLADEEIKVMKSEFLVKEERIAKKEENLEL 116 E+ K S +K E A +++ + + K + LE Sbjct: 194 ELEKALEGAMNFSTADSAKIKTLEAEKAALAARKADLEKALEGAMNFSTADSAKIKTLEA 253 Query: 117 RTNKLEEKEAKLEQRKEKIVEIENELNAMIEKEEKELER 155 LE ++A+LE+ E + +A I+ E E Sbjct: 254 EKAALEARQAELEKALEGAMNFSTADSAKIKTLEAEKAA 292

Smon_1021

Smon_1054

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
Smon_1021	2	9	1.244281	hypothetical protein
Smon_1022	1	10	3.332613	Tetrahydrodipicolinate succinyltransferase
Smon_1023	1	9	2.699938	oligoendopeptidase, M3 family
Smon_1024	1	12	2.892580	ATP synthase F1, epsilon subunit
Smon_1025	1	11	3.575220	ATP synthase F1, beta subunit
Smon_1026	0	9	1.900449	ATP synthase F1, gamma subunit
Smon_1027	-2	11	1.906335	ATP synthase F1, alpha subunit
Smon_1028	0	12	-0.315317	ATP synthase F1, delta subunit
Smon_1029	-1	9	0.864187	ATP synthase F0, B subunit
Smon_1030	1	8	0.293810	ATP synthase F0, C subunit
Smon_1031	3	8	-0.744990	ATP synthase F0, A subunit
Smon_1032	3	9	-1.868423	hypothetical protein
Smon_1033	2	12	-0.751989	conserved hypothetical protein
Smon_1034	-1	8	-0.650525	lysyl-tRNA synthetase
Smon_1035	-1	9	-1.990640	hypothetical protein
Smon_1036	-3	9	0.233290	hypothetical protein
Smon_1037	0	8	1.040287	protein of unknown function DUF163
Smon_1038	1	9	1.486623	putative RNA methylase
Smon_1041	1	9	2.367468	DNA mismatch repair protein MutL
Smon_1042	3	11	3.480604	hypothetical protein
Smon_1043	2	11	3.577449	extracellular solute-binding protein family 1
Smon_1044	3	9	3.782780	binding-protein-dependent transport systems
Smon_1045	2	8	3.446484	binding-protein-dependent transport systems
Smon_1046	0	8	3.654810	extracellular solute-binding protein family 1
Smon_1047	0	7	3.780836	N-acetylneuraminate lyase
Smon_1048	0	7	3.750262	ROK family protein
Smon_1049	0	11	5.128201	N-acylglucosamine-6-phosphate 2-epimerase
Smon_1050	0	11	3.741928	transcriptional regulator, RpiR family
Smon_1051	-2	11	3.140410	conserved hypothetical protein
Smon_1052	-2	11	3.221377	iron-containing alcohol dehydrogenase
Smon_1053	0	14	3.161079	endoribonuclease L-PSP
Smon_1054	0	14	3.195048	translation elongation factor Tu

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Smon_1048	PF03309	31	0.003	Bvg accessory factor
>PF03309#Bvg accessory factor Length = 271 Score = 31.3 bits (71), Expect = 0.003 Identities = 26/166 (15%), Positives = 59/166 (35%), Gaps = 30/166 (18%) Query: 3 IICFDIGGTNIKYAI-------IEDISNIEVKTIETRITKDDNYILEDVLKIIELN-KDV 54 ++ D+ T+ + + + ++T E +T D+ + + +I + + + Sbjct: 2 LLAIDVRNTHTVVGLISGSGDHAKVVQQWRIRT-EPEVTADELALT--IDGLIGDDAERL 58 Query: 55 KAVGISTAGVVNSKTGEVIFAGPTIPKYTGTKFKEIIE--AKFGIETFVEND-------- 104 + V S EV + +Y +IE + GI V+N Sbjct: 59 TGASGLS--TVPSVLHEVRVM---LEQYWPNVPHVLIEPGVRTGIPLLVDNPKEVGADRI 113 Query: 105 VNSAAFGEYCFGDYKGSMFMLTIGTGVGGSLILDGKVFSGASMTAG 150 VN A + Y + ++ G+ + ++ F G ++ G Sbjct: 114 VNCLA----AYHKYGTAAIVVDFGSSICVDVVSAKGEFLGGAIAPG 155
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Smon_1049	DHBDHDRGNASE	28	0.034	2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase signature. Length = 261 Score = 27.7 bits (61), Expect = 0.034 Identities = 20/88 (22%), Positives = 33/88 (37%), Gaps = 10/88 (11%) Query: 73 KGYEGFPQYIT-----VGMSEIDALVEAGADIIALDCTLRDRYDGKTINEFIADIKAKYP 127 KG EG +IT +G + L GA I A+D + + A +P Sbjct: 4 KGIEGKIAFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHAEAFP 63 Query: 128 HILLMADISNLEEGIN---AEKAGVDII 152 + D + ++E E +DI+ Sbjct: 64 --ADVRDSAAIDEITARIEREMGPIDIL 89
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Smon_1054	TCRTETOQM	83	2e-19	Tetracycline resistance protein TetO/TetQ/TetM family ...
>TCRTETOQM#Tetracycline resistance protein TetO/TetQ/TetM family signature. Length = 639 Score = 83.4 bits (206), Expect = 2e-19 Identities = 52/150 (34%), Positives = 79/150 (52%), Gaps = 7/150 (4%) Query: 13 VNVGTIGHVDHGKTTTTAAI---SKVLASKGLAQKVDFENIDQAPEERERGITINTAHIE 69 +N+G + HVD GKTT T ++ S + G K D ER+RGITI T Sbjct: 4 INIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGT-TRTDNTLLERQRGITIQTGITS 62 Query: 70 YESEARHYAHVDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQVGVP 129 ++ E +D PGH D++ + + +DGAIL++SA DG QTR R++G+P Sbjct: 63 FQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAKDGVQAQTRILFHALRKMGIP 122 Query: 130 YIVVYLNKVDMVEEEELLELVEMEVRELLS 159 I ++NK+D + L V +++E LS Sbjct: 123 TI-FFINKIDQNGID--LSTVYQDIKEKLS 149

Smon_1118

Smon_1143

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
Smon_1118	2	11	-0.197977	non-canonical purine NTP pyrophosphatase,
Smon_1119	2	11	-0.343542	regulatory protein RecX
Smon_1120	2	11	0.789225	recA protein
Smon_1121	2	9	-0.116227	oligoendopeptidase F
Smon_1122	-1	12	-0.541982	GHMP kinase
Smon_1123	-1	11	0.895478	diphosphomevalonate decarboxylase
Smon_1124	-2	10	0.940844	mevalonate kinase
Smon_1125	-3	9	0.622641	peroxiredoxin
Smon_1126	-2	8	-1.812495	hypothetical protein
Smon_1127	-1	6	-2.546488	hypothetical protein
Smon_1128	-1	8	-3.061081	glucose inhibited division protein A
Smon_1129	2	11	-6.201354	conserved hypothetical protein
Smon_1131	1	10	-5.018850	hypothetical protein
Smon_1132	-1	11	-4.002280	hypothetical protein
Smon_1134	-1	10	-1.260217	ABC transporter related protein
Smon_1136	-1	9	0.162056	conserved hypothetical protein
Smon_1138	0	8	0.374973	major facilitator superfamily MFS_1
Smon_1139	-1	9	1.081396	Cysteine desulfurase
Smon_1140	-1	10	-0.123131	SufBD protein
Smon_1141	0	8	0.344607	FeS assembly protein SufB
Smon_1142	0	8	-0.608460	FeS assembly ATPase SufC
Smon_1143	2	8	-0.211907	protein of unknown function DUF6 transmembrane

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Smon_1134	PF05272	32	0.006	Virulence-associated E family protein
>PF05272#Virulence-associated E family protein Length = 892 Score = 32.4 bits (73), Expect = 0.006 Identities = 11/31 (35%), Positives = 15/31 (48%) Query: 353 IVITGASGTGKSTLLKILTGEILNYDGDILL 383 +V+ G G GKSTL+ L G D + Sbjct: 599 VVLEGTGGIGKSTLINTLVGLDFFSDTHFDI 629
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Smon_1142	PF05272	32	0.002	Virulence-associated E family protein
>PF05272#Virulence-associated E family protein Length = 892 Score = 32.4 bits (73), Expect = 0.002 Identities = 15/52 (28%), Positives = 19/52 (36%), Gaps = 15/52 (28%) Query: 31 VHVIMGPNGAGKSTLASILIGHP--------------KYEVSQGKIVLE-GE 67 V+ G G GKSTL + L+G YE G + E E Sbjct: 598 SVVLEGTGGIGKSTLINTLVGLDFFSDTHFDIGTGKDSYEQIAGIVAYELSE 649

Smon_1158

Smon_1202

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
Smon_1158	3	14	-2.201401	hypothetical protein
Smon_1159	5	13	-1.907370	domain of unknown function DUF1738
Smon_1160	7	15	-4.568494	hypothetical protein
Smon_1161	4	13	-4.720996	hypothetical protein
Smon_1162	4	15	-2.109691	hypothetical protein
Smon_1163	3	14	-2.338835	hypothetical protein
Smon_1164	4	14	-2.617667	NLP/P60 protein
Smon_1165	6	14	-2.143830	hypothetical protein
Smon_1166	6	15	-1.511090	Outer membrane protein and related
Smon_1167	7	15	-1.482242	hypothetical protein
Smon_1168	3	13	-3.377245	hypothetical protein
Smon_1169	4	12	-3.000587	hypothetical protein
Smon_1170	-1	11	0.068309	hypothetical protein
Smon_1171	-4	11	0.477921	hypothetical protein
Smon_1172	-3	9	-0.089049	Type IV secretory pathway protease TraF-like
Smon_1173	-2	9	0.011256	hypothetical protein
Smon_1174	-3	9	-0.495206	conserved hypothetical protein
Smon_1175	-3	10	-0.810106	hypothetical protein
Smon_1176	-2	9	-2.720313	hypothetical protein
Smon_1177	-2	10	-3.270710	CagE TrbE VirB component of type IV transporter
Smon_1178	0	11	-3.363214	hypothetical protein
Smon_1179	1	11	-3.330470	hypothetical protein
Smon_1180	-1	10	-1.110777	hypothetical protein
Smon_1181	-1	9	-0.642847	hypothetical protein
Smon_1182	-2	8	-0.132373	YadA domain protein
Smon_1183	-2	8	-0.277581	type II secretion system protein E
Smon_1184	0	8	-1.058991	conserved hypothetical protein
Smon_1185	1	9	-1.253971	TRAG family protein
Smon_1186	5	10	-3.126608	conjugation TrbI family protein
Smon_1187	5	14	-4.432358	Conjugal transfer protein TrbG/VirB9/CagX
Smon_1188	8	20	-5.516431	hypothetical protein
Smon_1189	7	18	-5.201611	conserved hypothetical protein
Smon_1190	4	19	-5.951197	hypothetical protein
Smon_1191	4	16	-5.848772	hypothetical protein
Smon_1192	3	14	-6.120274	hypothetical protein
Smon_1193	4	13	-6.520582	MobA/MobL protein
Smon_1194	5	15	-7.036134	conserved hypothetical LOC616002
Smon_1195	5	14	-7.308393	hypothetical protein
Smon_1196	5	15	-5.844500	prophage antirepressor
Smon_1197	3	14	-5.846405	hypothetical protein
Smon_1198	-1	13	-4.018547	replication initiator A domain protein
Smon_1199	0	13	-3.687544	hypothetical protein
Smon_1200	-2	13	-2.320389	transcriptional regulator, XRE family
Smon_1201	-1	13	-2.097559	hypothetical protein
Smon_1202	-1	10	-3.061898	integrase family protein

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Smon_1166	OMPADOMAIN	68	3e-16	OMPA domain signature.
>OMPADOMAIN#OMPA domain signature. Length = 346 Score = 67.6 bits (165), Expect = 3e-16 Identities = 31/68 (45%), Positives = 38/68 (55%), Gaps = 5/68 (7%) Query: 89 FNSNKFTLNKNQMAIIDALITNLENKE-----IIVVGYTDTDGNDKYNLNLGLSRANSVK 143 FN NK TL A +D L + L N + ++V+GYTD G+D YN L RA SV Sbjct: 223 FNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLSERRAQSVV 282 Query: 144 AYLESKGI 151 YL SKGI Sbjct: 283 DYLISKGI 290
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Smon_1175	PF05844	31	0.010	YopD protein
>PF05844#YopD protein Length = 295 Score = 31.1 bits (70), Expect = 0.010 Identities = 19/62 (30%), Positives = 24/62 (38%), Gaps = 7/62 (11%) Query: 281 TAAVGGAVVGASAIKGGLSKGTAAFKDGKNMKG--IFNAAIKGMKEGSTIAKSGRLGKLG 338 A + G ASA+ G L A K+GK + I G E AK LGK Sbjct: 123 MAVIAGVGALASAVVGSLG----ALKNGKAISQEKTLQKNIDGRNELID-AKMQALGKTS 177 Query: 339 GK 340 + Sbjct: 178 DE 179
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Smon_1182	PF03895	27	0.026	Serum resistance protein DsrA.
>PF03895#Serum resistance protein DsrA. Length = 79 Score = 26.7 bits (59), Expect = 0.026 Identities = 18/75 (24%), Positives = 33/75 (44%), Gaps = 2/75 (2%) Query: 116 ANSGVASAIATASTIKNLGNKKHTISGSIGYYGKEVAGAIAYSTHY-KNFGFGANASFN- 173 +G+A+ A + ++ G K ++S ++G Y + A AI + F A +FN Sbjct: 5 LQTGLANQSALSMLVQPNGVGKTSVSAAVGGYRDKTALAIGVGSRITDRFTAKAGVAFNT 64 Query: 174 SRLEVGAGLGLSYTF 188 + G + Y F Sbjct: 65 YNGGMSYGASVGYEF 79
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Smon_1189	SALVRPPROT	27	0.047	Salmonella virulence-associated 28kDa protein signature.
>SALVRPPROT#Salmonella virulence-associated 28kDa protein signature. Length = 241 Score = 26.6 bits (58), Expect = 0.047 Identities = 15/73 (20%), Positives = 35/73 (47%) Query: 2 GYEEYCLWKRKNILKIINNEIDVFVYDVEKNIDELNKFLGDEKTIDELKDYEKRAEKDEE 61 G + + ++ N+ DVF++ ++ KF GD+ I L+D +A + Sbjct: 63 GMRQSGFFAMSQGFQLNNHGYDVFIHARRESPQSQGKFAGDKFHISVLRDMVPQAFQALS 122 Query: 62 KIFYQEENIINKF 74 + + E++ ++K+ Sbjct: 123 GLLFSEDSPVDKW 135
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Smon_1190	PF04335	34	2e-04	VirB8 type IV secretion protein
>PF04335#VirB8 type IV secretion protein Length = 227 Score = 34.4 bits (79), Expect = 2e-04 Identities = 31/214 (14%), Positives = 66/214 (30%), Gaps = 24/214 (11%) Query: 32 ERYINISESLNT-WKKAFIGMSILSLMLGGVSTFLFISKTETKSYLIKVNENN---ELVG 87 ++ S W A + ++ + + V L KT + Y+I V+ N + Sbjct: 23 DKLAAAERSKKLAWVVAGVAGALATAGVVAV-AALTPLKT-VEPYVITVDRNTGEASIAA 80 Query: 88 AEKLTNQISNIGNREIEYFMKKFIKDTRTITLDKKVFDKTIKEANY-----FLNKETQSK 142 I+ +YF+ +++ ++ + +E + + Q + Sbjct: 81 KLHGDATITY-DEAVRKYFLATYVRY-------REGWIAAAREEYFDAVMVMSARPEQDR 132 Query: 143 LGSILGSENINSFFE---NKKTREVEILSFVAIPEVEKTYQIRWREKYYGTSGELEKRKN 199 ++N S N+ VEI + Q+ + ++ S + Sbjct: 133 WSRFYKTDNPQSPQNILANRTDVFVEIKRVSFLGG--NVAQVYFTKESVTGSNSTKTDAV 190 Query: 200 LNAIVKIRNFSPNSEQIMLNPFGIIVVDFNMQVE 233 K+ NP G V + VE Sbjct: 191 ATIKYKVDGTPSKEVDRFKNPLGYQVESYRADVE 224
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Smon_1192	PREPILNPTASE	27	0.015	Type IV prepilin cysteine protease (C20) family sig...
>PREPILNPTASE#Type IV prepilin cysteine protease (C20) family signature. Length = 290 Score = 27.5 bits (61), Expect = 0.015 Identities = 24/87 (27%), Positives = 37/87 (42%), Gaps = 1/87 (1%) Query: 10 TKYLILLGVLFLSTLALADTNKVSVVGFDKAATYFVSVFGFVKFLGYISASIYFIFKLLE 69 T L+ G+LF + L + +V+G S++ K L Y FKLL Sbjct: 162 TLPLLWGGLLF-NLLGGFVSLGDAVIGAMAGYLVLWSLYWAFKLLTGKEGMGYGDFKLLA 220 Query: 70 FITSQQWDQLLKSFLIFAVIVGAIYGI 96 + + Q L L+ + +VGA GI Sbjct: 221 ALGAWLGWQALPIVLLLSSLVGAFMGI 247

Smon_1377

Smon_1394

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
Smon_1377	2	7	1.212609	Methyltransferase type 12
Smon_1378	3	8	2.216846	phosphoglucomutase/phosphomannomutase
Smon_1379	3	8	1.478246	Peptidase M23
Smon_1380	0	10	-0.713778	Cell division protein-like protein
Smon_1381	1	9	-2.167578	Transketolase central region
Smon_1382	1	9	-3.321182	Transketolase domain protein
Smon_1383	1	9	-4.420778	major facilitator superfamily MFS_1
Smon_1384	0	9	-5.433923	two component transcriptional regulator, LuxR
Smon_1385	1	9	-4.996473	Signal transduction histidine kinase-like
Smon_1386	-1	8	-2.821755	protein of unknown function DUF214
Smon_1387	2	10	-0.490706	ABC transporter related protein
Smon_1389	1	10	-0.343978	ABC transporter family protein
Smon_1390	1	10	-0.162107	hypothetical protein
Smon_1391	1	9	-0.954811	cytidyltransferase-related domain protein
Smon_1392	1	9	-1.493390	NADH:flavin oxidoreductase/NADH oxidase
Smon_1393	1	8	-2.088782	alpha amylase catalytic region
Smon_1394	-1	7	-3.217484	hypothetical protein

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Smon_1379	GPOSANCHOR	34	9e-04	Gram-positive coccus surface protein anchor signature.
>GPOSANCHOR#Gram-positive coccus surface protein anchor signature. Length = 539 Score = 34.3 bits (78), Expect = 9e-04 Identities = 50/295 (16%), Positives = 114/295 (38%), Gaps = 22/295 (7%) Query: 13 SLASFGNNIDKNKNRINQIDKQVKDNTNKINNNNSKINNAKKDEAAIKKEIQELDALISK 72 + + R ++K ++ N +++KI + ++AA++ EL+ + Sbjct: 212 KIKTLEAEKAALAARKADLEKALEGAMNFSTADSAKIKTLEAEKAALEARQAELEKALEG 271 Query: 73 LQREYNVIQNEYVSLLKEIGKSEKEIRS--SIRKIEESSKKITEGKTDYSNKINTWNKVF 130 + +L E E E ++ ++++ D S + + Sbjct: 272 AMNFSTADSAKIKTLEAEKAALEAEKADLEHQSQVLNANRQSLRRDLDASREAKKQLEAE 331 Query: 131 NAKVFQKNSFSSESAKKTNDLIKVLE----QGQNNIKKIEKYKQQEEIHKKNEEILKNKT 186 + K+ ++N S S + + Q + +K+E+ + E +++ + + Sbjct: 332 HQKLEEQNKISEASRQSLRRDLDASREAKKQLEAEHQKLEEQNKISEASRQSLRRDLDAS 391 Query: 187 QKEAKKVEKKKLELENKREELRKAKINKDRAVKNLQNLQDSLKDENKKIERTNSSLIAEK 246 ++ K+VEK E +K L K + L++S K K+ + L AE Sbjct: 392 REAKKQVEKALEEANSKLAALEKL----------NKELEESKKLTEKEKAELQAKLEAEA 441 Query: 247 RRLEQQINAIIAAAKKREEDARKRAQGSNKNQSGNESTDKKEPIKAVVVPKGTGK 301 + L++++ AK+ EE A+ RA ++ +Q+ + K P+ K Sbjct: 442 KALKEKL------AKQAEELAKLRAGKASDSQTPDAKPGNKAVPGKGQAPQAGTK 490 Score = 31.6 bits (71), Expect = 0.006 Identities = 30/243 (12%), Positives = 79/243 (32%), Gaps = 17/243 (6%) Query: 23 KNKNRINQIDKQVKDNTNKINNNNSKINNAKKDEAAIKKEIQELDALISKLQREYNVIQN 82 + +I ++ + + + + A A +I+ L+A + L+ ++ Sbjct: 138 ADSAKIKTLEAEKAALAARKADLEKALEGAMNFSTADSAKIKTLEAEKAALEARQAELEK 197 Query: 83 EYVSLLKEIGKSE---KEIRSSIRKIEESSKKITEGKTDYSNKINTWNKVFNAKVFQKNS 139 + K + + + + + N + +K + Sbjct: 198 ALEGAMNFSTADSAKIKTLEAEKAALAARKADLEKALEGAMNFSTADSAKIKTLEAEKAA 257 Query: 140 FSSESA---KKTNDLIKVLEQGQNNIKKIEKYKQQ---------EEIHKKNEEILKNKTQ 187 + A K + IK +E K + N + Sbjct: 258 LEARQAELEKALEGAMNFSTADSAKIKTLEAEKAALEAEKADLEHQSQVLNANRQSLRRD 317 Query: 188 KEAKKVEKKKLELENKREELRKAKINKDRAVKNLQNLQDSLKDENKKIERTNSSLIAEKR 247 +A + KK+LE E++ +L + + + ++L+ D+ ++ K++E + L + + Sbjct: 318 LDASREAKKQLEAEHQ--KLEEQNKISEASRQSLRRDLDASREAKKQLEAEHQKLEEQNK 375 Query: 248 RLE 250 E Sbjct: 376 ISE 378
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Smon_1380	TYPE3IMSPROT	29	0.020	Type III secretion system inner membrane S protein ...
>TYPE3IMSPROT#Type III secretion system inner membrane S protein family signature. Length = 354 Score = 29.3 bits (66), Expect = 0.020 Identities = 16/97 (16%), Positives = 37/97 (38%), Gaps = 9/97 (9%) Query: 211 FLESVLAVLISGAVSYIMYVKIRCSIVGLIEKARGATAIQGFSTLGKETNFLFIVLIITI 270 FL+S+L V++ + +I+ +++ L G + + L++ Sbjct: 140 FLKSILKVVLLSILIWIIIKGNLVTLLQL--------PTCGIECITPLLGQILRQLMVIC 191 Query: 271 ILVLVINYLFLNKYYNIRYYEKNKNEEIQEVKEEVEE 307 + V+ + + + Y K E+K E +E Sbjct: 192 TVGFVVISIA-DYAFEYYQYIKELKMSKDEIKREYKE 227
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Smon_1383	TCRTETA	36	3e-04	Tetracycline resistance protein signature.
>TCRTETA#Tetracycline resistance protein signature. Length = 399 Score = 35.6 bits (82), Expect = 3e-04 Identities = 58/353 (16%), Positives = 119/353 (33%), Gaps = 43/353 (12%) Query: 34 YLPSEKAIADYGISVIMPPVYLAIAFVIMRFVDAIADPVVGYMSDNSTSSLGRRSFYMLI 93 + S A YG L + +M+F PV+G +SD GRR + Sbjct: 35 LVHSNDVTAHYG--------ILLALYALMQF---ACAPVLGALSD----RFGRR----PV 75 Query: 94 GIIPLALSMIAFFFPFNNGPLIATLYLAFVGSIYFIAYTLVGGPYNALIPDLASNKEERL 153 ++ LA + + + P + LY+ G I G A I D+ E Sbjct: 76 LLVSLAGAAVDYAI-MATAPFLWVLYI---GRIVAGITGATGAVAGAYIADITDGDERAR 131 Query: 154 NLSTIQSVFRLIFTAIPLIFSPIILSNMIKSGMSFIKAMRLMVTGFSVLSAIIVIISVML 213 + + + F A P++ L F A + L+ + + L Sbjct: 132 HFGFMSACFGFGMVAGPVLGG---LMGGFSPHAPFFAA--------AALNGLNFLTGCFL 180 Query: 214 LKENKVRHNNNNDVKKINFKEAFSYLKNKEIILYFIGFFFFFSGFNIIRNSV-LYYVTVI 272 L E+ + +N +F + + ++ + FF + ++ + + Sbjct: 181 LPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIMQLVGQVPAALWVIFGEDR 240 Query: 273 LGQSEKAASLPTTILFAMSALF-FPVTQFLSKKFDYRKVMLFDLALIILGTLGLIFLGDK 331 + + +L +T ++ + R+ ++ + G + L F Sbjct: 241 FHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALMLGMIADGTGYILLAFATR- 299 Query: 332 SKSIFFAMFLVVGAGVSGSAFIFPPAMLSEISIKLHEKYNVSVEGMMFGIQGL 384 F M L+ G I PA+ + +S ++ E+ ++G + + L Sbjct: 300 GWMAFPIMVLLASGG------IGMPALQAMLSRQVDEERQGQLQGSLAALTSL 346
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Smon_1384	HTHFIS	48	6e-09	FIS bacterial regulatory protein HTH signature.
>HTHFIS#FIS bacterial regulatory protein HTH signature. Length = 484 Score = 47.9 bits (114), Expect = 6e-09 Identities = 21/117 (17%), Positives = 49/117 (41%), Gaps = 6/117 (5%) Query: 2 KILLIDDHKLFALSIQMILGKYKEIERIDVITDSKQ-LEKKDIKDYDIYLIDINLNNISE 60 IL+ DD + L + + + +++ D D+ + D+ + + Sbjct: 5 TILVADDDAAIRTVLNQALSR--AGYDVRITSNAATLWRWIAAGDGDLVVTDVVM---PD 59 Query: 61 ETGLELAEKLIKKDKNICIVILTGHLKLMYEDKANKIGVRGFIDKNIDPEELIKILK 117 E +L ++ K ++ +++++ M KA++ G ++ K D ELI I+ Sbjct: 60 ENAFDLLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIG 116

Smon_1408

Smon_1418

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
Smon_1408	-2	10	3.145938	YadA domain protein
Smon_1412	5	25	4.155243	phospholipase D/Transphosphatidylase
Smon_1413	7	33	5.808138	hypothetical protein
Smon_1415	8	34	6.266126	hypothetical protein
Smon_1416	6	28	5.855343	protein of unknown function DUF87
Smon_1417	4	24	4.327882	conserved hypothetical protein
Smon_1418	3	23	4.198378	type III restriction protein res subunit

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Smon_1408	PF03895	51	8e-10	Serum resistance protein DsrA.
>PF03895#Serum resistance protein DsrA. Length = 79 Score = 50.6 bits (121), Expect = 8e-10 Identities = 23/74 (31%), Positives = 38/74 (51%), Gaps = 3/74 (4%) Query: 1821 GGVANAIAVASIPQINGKGH-NIGASYGYYEGHSAFALGL-SGINERGNVLYKANLSLNT 1878 G+AN A++ + Q NG G ++ A+ G Y +A A+G+ S I +R + Sbjct: 7 TGLANQSALSMLVQPNGVGKTSVSAAVGGYRDKTALAIGVGSRITDRFTAKAGVAFNTY- 65 Query: 1879 RGNVGIGAGIGYQF 1892 G + GA +GY+F Sbjct: 66 NGGMSYGASVGYEF 79

Smon_0171

Smon_0190

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
Smon_0171	0	11	3.160988	YadA domain protein
Smon_0172	0	14	3.015270	hypothetical protein
Smon_0173	0	14	3.499293	YadA domain protein
Smon_0174	0	16	3.408198	YadA domain protein
Smon_0175	2	17	4.032877	hypothetical protein
Smon_0176	2	16	4.192937	Hemagluttinin domain protein
Smon_0177	2	15	4.240601	YadA domain protein
Smon_0178	3	14	4.600367	YadA domain protein
Smon_0179	2	14	4.770917	YadA domain protein
Smon_0180	2	11	4.744100	YadA domain protein
Smon_0181	-1	8	1.964593	Arginine deiminase
Smon_0182	-1	7	1.881828	ornithine carbamoyltransferase
Smon_0183	-1	7	1.762572	carbamate kinase
Smon_0184	-1	8	2.491639	C4-dicarboxylate anaerobic carrier
Smon_0185	0	9	2.783925	peptidase U34 dipeptidase
Smon_0186	0	11	2.641874	peptidase S6 IgA endopeptidase
Smon_0187	3	23	6.917797	ribosomal protein S12
Smon_0188	4	24	6.514933	ribosomal protein S7
Smon_0189	3	25	6.277850	translation elongation factor G
Smon_0190	1	22	4.722880	translation elongation factor Tu

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Smon_0171	OMADHESIN	50	3e-08	Yersinia outer membrane adhesin signature.
>OMADHESIN#Yersinia outer membrane adhesin signature. Length = 455 Score = 50.3 bits (119), Expect = 3e-08 Identities = 61/204 (29%), Positives = 91/204 (44%), Gaps = 29/204 (14%) Query: 249 GYVTSAKAHSTVAIGYYAKALKSSATAIGSQAEASGQVSTAIGATAKATETYAVSIGAKS 308 G SAK ++AIG A+A K +A A+G+ + A+G S AIG +KA AV+ GA S Sbjct: 62 GLNASAKGIHSIAIGATAEAAKGAAVAVGAGSIATGVNSVAIGPLSKALGDSAVTYGAAS 121 Query: 309 EASFKG------------SVAIGSGSKTDSKATKETEATVNKITYSGFAGSNPDEGYVVS 356 A G VA+G SK D+K N + + + GY ++ Sbjct: 122 TAQKDGVAIGARASTSDTGVAVGFNSKADAK---------NSVAIGHSSHVAANHGYSIA 172 Query: 357 VGTEIPSTDKSNSGKI----IKRQIKNVAAGKISKESTDAINGSQLYMTNNVLGNLADST 412 +G + TD+ NS I + RQ+ ++AAG + TDA+N +QL + Sbjct: 173 IG-DRSKTDRENSVSIGHESLNRQLTHLAAG---TKDTDAVNVAQLKKEIEKTQENTNKR 228 Query: 413 KTILGGNASITTSGNDAGKLTITN 436 L NA+ + L I N Sbjct: 229 SAELLANANAYADNKSSSVLGIAN 252 Score = 46.8 bits (110), Expect = 4e-07 Identities = 63/276 (22%), Positives = 105/276 (38%), Gaps = 12/276 (4%) Query: 72 GIRSKADGEKTIVVGVDSSSKSKESVVIGYKSTTEKENSVAIGSNSNVTGKNSVAVGSDT 131 G+ + A G +I +G + + +V +G S NSVAIG S G ++V G+ + Sbjct: 62 GLNASAKGIHSIAIGATAEAAKGAAVAVGAGSIATGVNSVAIGPLSKALGDSAVTYGAAS 121 Query: 132 KVEGSGASAFGYKANAKGESSVAFGVDTTASGLRSVAIGKGAMATKNDD--IAVGSGAKT 189 + G A G +A+ ++ VA G ++ A SVAIG + N IA+G +KT Sbjct: 122 TAQKDGV-AIGARASTS-DTGVAVGFNSKADAKNSVAIGHSSHVAANHGYSIAIGDRSKT 179 Query: 190 NTRN---ITMKDNQESKSSLAFGINATADGVNTISIGTGTKAEQSSAIAIGGEAAGVGAV 246 + N I + + LA G T D VN + + Q + E Sbjct: 180 DRENSVSIGHESLNRQLTHLAAGTKDT-DAVNVAQLKKEIEKTQENTNKRSAELLA---- 234 Query: 247 TIGYVTSAKAHSTVAIGYYAKALKSSATAIGSQAEASGQVSTAIGATAKATETYAVSIGA 306 K+ S + I KS+ T ++ EA Q + + + A + Sbjct: 235 NANAYADNKSSSVLGIANNYTDSKSAETLENARKEAFAQSKDVLNMAKAHSNSVARTTLE 294 Query: 307 KSEASFKGSVAIGSGSKTDSKATKETEATVNKITYS 342 +E + + K EA + Y+ Sbjct: 295 TAEEHANSVARTTLETAEEHANKKSAEALASANVYA 330 Score = 44.9 bits (105), Expect = 2e-06 Identities = 53/172 (30%), Positives = 79/172 (45%), Gaps = 29/172 (16%) Query: 140 AFGYKANAKGESSVAFGVDTTASGLRSVAIGKGAMATKNDDIAVGSGAKTNTRNITMKDN 199 A G + + A G++ +A G+ S+AIG A A K +AVG+G Sbjct: 46 ALGLEYPVRPPVPGAGGLNASAKGIHSIAIGATAEAAKGAAVAVGAG------------- 92 Query: 200 QESKSSLAFGINATADGVNTISIGTGTKAEQSSAIAIG-GEAAGVGAVTIGYVTSAKAHS 258 + A GVN+++IG +KA SA+ G A V IG S + + Sbjct: 93 ------------SIATGVNSVAIGPLSKALGDSAVTYGAASTAQKDGVAIGARAST-SDT 139 Query: 259 TVAIGYYAKALKSSATAIG--SQAEASGQVSTAIGATAKATETYAVSIGAKS 308 VA+G+ +KA ++ AIG S A+ S AIG +K +VSIG +S Sbjct: 140 GVAVGFNSKADAKNSVAIGHSSHVAANHGYSIAIGDRSKTDRENSVSIGHES 191 Score = 39.9 bits (92), Expect = 6e-05 Identities = 27/73 (36%), Positives = 41/73 (56%), Gaps = 2/73 (2%) Query: 47 AASNPATDGIAQGAEVTATKDSAVFGIRSKADGEKTIVVGVDS--SSKSKESVVIGYKST 104 AAS DG+A GA + + G SKAD + ++ +G S ++ S+ IG +S Sbjct: 119 AASTAQKDGVAIGARASTSDTGVAVGFNSKADAKNSVAIGHSSHVAANHGYSIAIGDRSK 178 Query: 105 TEKENSVAIGSNS 117 T++ENSV+IG S Sbjct: 179 TDRENSVSIGHES 191
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Smon_0173	PF03895	50	2e-09	Serum resistance protein DsrA.
>PF03895#Serum resistance protein DsrA. Length = 79 Score = 49.8 bits (119), Expect = 2e-09 Identities = 23/74 (31%), Positives = 38/74 (51%), Gaps = 3/74 (4%) Query: 1821 GGVANAIAVASIPQINGKGH-NIGASYGYYEGHSAFALGL-SGINERGNVLYKANLSLNT 1878 G+AN A++ + Q NG G ++ A+ G Y +A A+G+ S I +R + Sbjct: 7 TGLANQSALSMLVQPNGVGKTSVSAAVGGYRDKTALAIGVGSRITDRFTAKAGVAFNTY- 65 Query: 1879 RGNVGIGAGIGYQF 1892 G + GA +GY+F Sbjct: 66 NGGMSYGASVGYEF 79
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Smon_0174	PF03895	51	9e-10	Serum resistance protein DsrA.
>PF03895#Serum resistance protein DsrA. Length = 79 Score = 50.6 bits (121), Expect = 9e-10 Identities = 23/74 (31%), Positives = 38/74 (51%), Gaps = 3/74 (4%) Query: 1834 GGVANAIAVASIPQINGKGH-NIGASYGYYEGHSAFALGL-SGINERGNVLYKANLSLNT 1891 G+AN A++ + Q NG G ++ A+ G Y +A A+G+ S I +R + Sbjct: 7 TGLANQSALSMLVQPNGVGKTSVSAAVGGYRDKTALAIGVGSRITDRFTAKAGVAFNTY- 65 Query: 1892 RGNVGIGAGIGYQF 1905 G + GA +GY+F Sbjct: 66 NGGMSYGASVGYEF 79
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Smon_0176	OMADHESIN	45	7e-08	Yersinia outer membrane adhesin signature.
>OMADHESIN#Yersinia outer membrane adhesin signature. Length = 455 Score = 45.3 bits (106), Expect = 7e-08 Identities = 45/147 (30%), Positives = 69/147 (46%), Gaps = 22/147 (14%) Query: 8 SIAYGDGSKATAPNSIVLGIKSYILGDKNQGDSIIIGNNAYIYSLYGSSNNKNGHNAKSV 67 ++A G GS AT NS+ +G S K GDS + A G + + + Sbjct: 86 AVAVGAGSIATGVNSVAIGPLS-----KALGDSAVTYGAASTAQKDGVAIGARASTSDTG 140 Query: 68 LALGNETLATLDNSVALGHSSQTDYIQSDLNKPGYTARGSYSI-----PSSAKVGVISVG 122 +A+G + A NSVA+GHSS A YSI + + +S+G Sbjct: 141 VAVGFNSKADAKNSVAIGHSSHV------------AANHGYSIAIGDRSKTDRENSVSIG 188 Query: 123 KKGYERRIINVADGYRDSDAVNVSQLK 149 + R++ ++A G +D+DAVNV+QLK Sbjct: 189 HESLNRQLTHLAAGTKDTDAVNVAQLK 215
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Smon_0177	PF03895	48	4e-09	Serum resistance protein DsrA.
>PF03895#Serum resistance protein DsrA. Length = 79 Score = 47.9 bits (114), Expect = 4e-09 Identities = 21/76 (27%), Positives = 37/76 (48%), Gaps = 3/76 (3%) Query: 874 TSGGIANAIARANLPQISGKGH-NIAGSYGYYNGEHAFALGL-SGTNEVSNLVYRASGSL 931 G+AN A + L Q +G G +++ + G Y + A A+G+ S + + + Sbjct: 5 LQTGLANQSALSMLVQPNGVGKTSVSAAVGGYRDKTALAIGVGSRITDRFTAKAGVAFNT 64 Query: 932 NTRGHVSLGAGLGYQF 947 G +S GA +GY+F Sbjct: 65 Y-NGGMSYGASVGYEF 79
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Smon_0178	OMADHESIN	63	2e-12	Yersinia outer membrane adhesin signature.
>OMADHESIN#Yersinia outer membrane adhesin signature. Length = 455 Score = 63.0 bits (152), Expect = 2e-12 Identities = 51/148 (34%), Positives = 90/148 (60%), Gaps = 4/148 (2%) Query: 148 AIGLESWIQSKDGIAIGKKAKSAGEGAVALGSEAEATMRNAIALGNGAVALGENTVSLGG 207 A+GLE ++ A G A + G ++A+G+ AEA A+A+G G++A G N+V++G Sbjct: 46 ALGLEYPVRPPVPGAGGLNASAKGIHSIAIGATAEAAKGAAVAVGAGSIATGVNSVAIGP 105 Query: 208 DAMATSKNTIAIGINSKAEKESSIALGSGARSSKKYGISLGENSKSTGNSAISIGQKS-- 265 + A + + G S A+K+ +A+G+ A +S G+++G NSK+ ++++IG S Sbjct: 106 LSKALGDSAVTYGAASTAQKDG-VAIGARASTSDT-GVAVGFNSKADAKNSVAIGHSSHV 163 Query: 266 ISKNSNSIAIGTSATSNIENSVALGAES 293 + + SIAIG + ++ ENSV++G ES Sbjct: 164 AANHGYSIAIGDRSKTDRENSVSIGHES 191 Score = 61.8 bits (149), Expect = 3e-12 Identities = 70/267 (26%), Positives = 119/267 (44%), Gaps = 19/267 (7%) Query: 34 RAASVLTNTATGERAIALGEDAKASANRAIAIGEDSESSGQTSIAIGAYSKANSQAAVAI 93 A L +A G +IA+G A+A+ A+A+G S ++G S+AIG SKA +AV Sbjct: 58 PGAGGLNASAKGIHSIAIGATAEAAKGAAVAVGAGSIATGVNSVAIGPLSKALGDSAVTY 117 Query: 94 GEGAKASSDSSVAIGSLSKALENSSISIGKNSEVKKENSIAIGKEISVEGENAIAIGLES 153 G + A D VAIG+ + ++ +++G NS+ +NS+AIG V + + Sbjct: 118 GAASTAQKD-GVAIGARAST-SDTGVAVGFNSKADAKNSVAIGHSSHVAANHGYS----- 170 Query: 154 WIQSKDGIAIGKKAKSAGEGAVALGSEAEATMRNAIALG---NGAVALGENTVSLGGDAM 210 IAIG ++K+ E +V++G E+ +A G AV + + + Sbjct: 171 -------IAIGDRSKTDRENSVSIGHESLNRQLTHLAAGTKDTDAVNVAQLKKEIEKTQE 223 Query: 211 ATSKNT--IAIGINSKAEKESSIALGSGARSSKKYGISLGENSKSTGNSAISIGQKSISK 268 T+K + + N+ A+ +SS LG + EN++ + Sbjct: 224 NTNKRSAELLANANAYADNKSSSVLGIANNYTDSKSAETLENARKEAFAQSKDVLNMAKA 283 Query: 269 NSNSIAIGTSATSNIENSVALGAESET 295 +SNS+A T T+ + ET Sbjct: 284 HSNSVARTTLETAEEHANSVARTTLET 310 Score = 61.8 bits (149), Expect = 4e-12 Identities = 68/241 (28%), Positives = 118/241 (48%), Gaps = 16/241 (6%) Query: 134 AIGKEISVEGENAIAIGLESWIQSKDGIAIGKKAKSAGEGAVALGSEAEATMRNAIALGN 193 A+G E V A GL + + IAIG A++A AVA+G+ + AT N++A+G Sbjct: 46 ALGLEYPVRPPVPGAGGLNASAKGIHSIAIGATAEAAKGAAVAVGAGSIATGVNSVAIGP 105 Query: 194 GAVALGENTVSLGGDAMA------------TSKNTIAIGINSKAEKESSIALG--SGARS 239 + ALG++ V+ G + A TS +A+G NSKA+ ++S+A+G S + Sbjct: 106 LSKALGDSAVTYGAASTAQKDGVAIGARASTSDTGVAVGFNSKADAKNSVAIGHSSHVAA 165 Query: 240 SKKYGISLGENSKSTGNSAISIGQKSISKNSNSIAIGTSATSNIENSVALGAESETTVAK 299 + Y I++G+ SK+ +++SIG +S+++ +A GT T + N L E E T Sbjct: 166 NHGYSIAIGDRSKTDRENSVSIGHESLNRQLTHLAAGTKDTDAV-NVAQLKKEIEKTQEN 224 Query: 300 PTSEIVKPRFFLD-YKDFAGSNPYGVVSIGSKGKERQLQYVAAGQISKESTDAVNGSQLF 358 + + Y D S+ G+ + + K + A + +S D +N ++ Sbjct: 225 TNKRSAELLANANAYADNKSSSVLGIANNYTDSKSAETLENARKEAFAQSKDVLNMAKAH 284 Query: 359 S 359 S Sbjct: 285 S 285 Score = 31.4 bits (70), Expect = 0.013 Identities = 32/127 (25%), Positives = 54/127 (42%), Gaps = 12/127 (9%) Query: 26 GSNIIGNKRAASVLTNTATGERAIALGEDAKASANRAIAIGEDSESSGQ--TSIAIGAYS 83 G+ K ++ +T + +A+G ++KA A ++AIG S + SIAIG S Sbjct: 118 GAASTAQKDGVAIGARASTSDTGVAVGFNSKADAKNSVAIGHSSHVAANHGYSIAIGDRS 177 Query: 84 KANSQAAVAIGE----------GAKASSDSSVAIGSLSKALENSSISIGKNSEVKKENSI 133 K + + +V+IG A +V + L K +E + + K S N+ Sbjct: 178 KTDRENSVSIGHESLNRQLTHLAAGTKDTDAVNVAQLKKEIEKTQENTNKRSAELLANAN 237 Query: 134 AIGKEIS 140 A S Sbjct: 238 AYADNKS 244
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Smon_0179	OMADHESIN	75	4e-16	Yersinia outer membrane adhesin signature.
>OMADHESIN#Yersinia outer membrane adhesin signature. Length = 455 Score = 74.9 bits (183), Expect = 4e-16 Identities = 77/249 (30%), Positives = 119/249 (47%), Gaps = 48/249 (19%) Query: 113 GSHSSSGGAVFGVGIGFQARATKASAIAIGAGSYSNGYYSQAYGRSATALGNESIAQGVT 172 G ++S + + IG A A K +A+A+GAGS + G S A G + ALG+ ++ G Sbjct: 61 GGLNASAKGIHSIAIGATAEAAKGAAVAVGAGSIATGVNSVAIGPLSKALGDSAVTYGAA 120 Query: 173 SLAKEKGSIAIGTNATSSGENAIAIGSSARIRNALYQTDQSKRTDASGKNSVALGHMSQA 232 S A++ G +AIG A++S + +A+G +++ A KNSVA+GH S Sbjct: 121 STAQKDG-VAIGARASTS-DTGVAVGFNSK---------------ADAKNSVAIGHSSHV 163 Query: 233 SIENG--IAIGSESKTTVDKGIEGYNPNSSDTETLKGNVLKSTHAALAIGNGSTVTRQIT 290 + +G IAIG SKT + + +IG+ S + RQ+T Sbjct: 164 AANHGYSIAIGDRSKTDRENSV-------------------------SIGHES-LNRQLT 197 Query: 291 GLAAGKEDTDAVNVAQLKALEKKITNLSDDTINKWKEKLTIGYKVGSETNSKTVSLSTGL 350 LAAG +DTDAVNVAQLK K+I ++T + E L +S + ++ Sbjct: 198 HLAAGTKDTDAVNVAQLK---KEIEKTQENTNKRSAELLANANAYADNKSSSVLGIANNY 254 Query: 351 HFKSSNDNL 359 S + L Sbjct: 255 TDSKSAETL 263
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Smon_0180	OMADHESIN	64	2e-12	Yersinia outer membrane adhesin signature.
>OMADHESIN#Yersinia outer membrane adhesin signature. Length = 455 Score = 64.1 bits (155), Expect = 2e-12 Identities = 65/180 (36%), Positives = 100/180 (55%), Gaps = 30/180 (16%) Query: 564 GTKAEAQGEGSIVLGYHSKS-KENAISIGKESEALSIGSVSLGHSSKAKGERSVSIGAYA 622 G A A+G SI +G +++ K A+++G S A + SV++G SKA G+ +V+ GA + Sbjct: 62 GLNASAKGIHSIAIGATAEAAKGAAVAVGAGSIATGVNSVAIGPLSKALGDSAVTYGAAS 121 Query: 623 TAEKSNDIAIGLSSKASGGYSIAIGLSAKAEASSSTAIGINSK--ADIEDSVALGSESKT 680 TA+K + +AIG + S +A+G ++KA+A +S AIG +S A+ S+A+G SKT Sbjct: 122 TAQK-DGVAIGARASTSDT-GVAVGFNSKADAKNSVAIGHSSHVAANHGYSIAIGDRSKT 179 Query: 681 TKATSVSEVKIGELKYGEFAGKNPMSVVSIGTKNKERQLQHLAAGQISKESTDAINGSQL 740 + S VSIG ++ RQL HLAAG + TDA+N +QL Sbjct: 180 DRENS----------------------VSIGHESLNRQLTHLAAG---TKDTDAVNVAQL 214 Score = 59.1 bits (142), Expect = 6e-11 Identities = 47/158 (29%), Positives = 83/158 (52%), Gaps = 12/158 (7%) Query: 450 GHSSKSEGNHSISIGKDSKASDLDSVALGHQARSTGSRSTALGPHSEATKDNALALGVWS 509 G ++ ++G HSI+IG ++A+ +VA+G + +TG S A+GP S+A D+A+ G S Sbjct: 62 GLNASAKGIHSIAIGATAEAAKGAAVAVGAGSIATGVNSVAIGPLSKALGDSAVTYGAAS 121 Query: 510 KATMQG------------AIAIGSNSTSNASSAITIGENSKVETGAENSIAIGKEAKSLK 557 A G +A+G NS ++A +++ IG +S V SIAIG +K+ + Sbjct: 122 TAQKDGVAIGARASTSDTGVAVGFNSKADAKNSVAIGHSSHVAANHGYSIAIGDRSKTDR 181 Query: 558 KDSIVLGTKAEAQGEGSIVLGYHSKSKENAISIGKESE 595 ++S+ +G ++ + + G N + KE E Sbjct: 182 ENSVSIGHESLNRQLTHLAAGTKDTDAVNVAQLKKEIE 219 Score = 51.8 bits (123), Expect = 1e-08 Identities = 51/162 (31%), Positives = 82/162 (50%), Gaps = 16/162 (9%) Query: 405 GKMGDSAGAIGDGSITAGAQAKADGDNAIAIGKKAETKQSSGIAIGHSSKSEGNHSISIG 464 G G +A A G SI GA A+A A+A+G + + +AIG SK+ G+ +++ G Sbjct: 59 GAGGLNASAKGIHSIAIGATAEAAKGAAVAVGAGSIATGVNSVAIGPLSKALGDSAVTYG 118 Query: 465 KDSKASDLDSVALGHQARSTGSRSTALGPHSEATKDNALALGVWSKATMQGAIAIGSNST 524 S A D VA+G +A +T D +A+G SKA + ++AIG +S Sbjct: 119 AASTAQK-DGVAIGARA---------------STSDTGVAVGFNSKADAKNSVAIGHSSH 162 Query: 525 SNASSAITIGENSKVETGAENSIAIGKEAKSLKKDSIVLGTK 566 A+ +I + +T ENS++IG E+ + + + GTK Sbjct: 163 VAANHGYSIAIGDRSKTDRENSVSIGHESLNRQLTHLAAGTK 204 Score = 51.1 bits (121), Expect = 2e-08 Identities = 45/139 (32%), Positives = 70/139 (50%), Gaps = 8/139 (5%) Query: 102 GTDAKATATDAIAIGNKANARTKYGIAIGTETKTDGIASIALGYKSDANSDS-IAIGKEA 160 G +A A +IAIG A A +A+G + G+ S+A+G S A DS + G + Sbjct: 62 GLNASAKGIHSIAIGATAEAAKGAAVAVGAGSIATGVNSVAIGPLSKALGDSAVTYGAAS 121 Query: 161 VGYGKGVALGENAHSRGRSVAIG--HKADSKGVYSYGNVVIGNESTTNENLVYSTALGSG 218 GVA+G A + VA+G KAD+K +V IG+ S N YS A+G Sbjct: 122 TAQKDGVAIGARASTSDTGVAVGFNSKADAK-----NSVAIGHSSHVAANHGYSIAIGDR 176 Query: 219 AKVEANYSTALGSKSIAKK 237 +K + S ++G +S+ ++ Sbjct: 177 SKTDRENSVSIGHESLNRQ 195 Score = 46.4 bits (109), Expect = 5e-07 Identities = 44/150 (29%), Positives = 73/150 (48%), Gaps = 21/150 (14%) Query: 38 GLNSSTNNEEKVKIGANAKADSEKSIAIGMDSTSRGKKGIAIGAGSLAGAEKHLSNEIED 97 GLN+S + IGA A+A ++A+G S + G +AIG LS + D Sbjct: 62 GLNASAKGIHSIAIGATAEAAKGAAVAVGAGSIATGVNSVAIGP---------LSKALGD 112 Query: 98 TIAIGTDAKATATDAIAIGNKANARTKYGIAIGTETKTDGIASIALGYKSDANSDSIAIG 157 + A D +AIG +A+ + G+A+G +K D S+A+G+ S Sbjct: 113 SAVTYGAASTAQKDGVAIGARAST-SDTGVAVGFNSKADAKNSVAIGHSSHV-------- 163 Query: 158 KEAVGYGKGVALGENAHS-RGRSVAIGHKA 186 A +G +A+G+ + + R SV+IGH++ Sbjct: 164 --AANHGYSIAIGDRSKTDRENSVSIGHES 191 Score = 44.1 bits (103), Expect = 3e-06 Identities = 40/124 (32%), Positives = 65/124 (52%), Gaps = 6/124 (4%) Query: 30 EGEKSLFVGLNSSTNNEEKVKIGANAKADSEKSIAIGMDSTSRGKKGIAIGAGSLA---G 86 +G S+ +G + V +GA + A S+AIG S + G + GA S A G Sbjct: 68 KGIHSIAIGATAEAAKGAAVAVGAGSIATGVNSVAIGPLSKALGDSAVTYGAASTAQKDG 127 Query: 87 AEKHLSNEIEDT-IAIGTDAKATATDAIAIGNKAN--ARTKYGIAIGTETKTDGIASIAL 143 DT +A+G ++KA A +++AIG+ ++ A Y IAIG +KTD S+++ Sbjct: 128 VAIGARASTSDTGVAVGFNSKADAKNSVAIGHSSHVAANHGYSIAIGDRSKTDRENSVSI 187 Query: 144 GYKS 147 G++S Sbjct: 188 GHES 191 Score = 42.2 bits (98), Expect = 1e-05 Identities = 60/239 (25%), Positives = 99/239 (41%), Gaps = 25/239 (10%) Query: 407 MGDSAGAIGDGS------ITAGAQAKADGDNAIAIGKKAETKQSSGIAIGHSSKSEGNH- 459 +GDSA G S + GA+A D +A+G ++ + +AIGHSS NH Sbjct: 110 LGDSAVTYGAASTAQKDGVAIGARASTS-DTGVAVGFNSKADAKNSVAIGHSSHVAANHG 168 Query: 460 -SISIGKDSKASDLDSVALGHQARSTGSRSTALGPHSEATKDNALALGVWSKATMQGAIA 518 SI+IG SK +SV++GH++ + A G TKD + A ++ I Sbjct: 169 YSIAIGDRSKTDRENSVSIGHESLNRQLTHLAAG-----TKDTDAV----NVAQLKKEIE 219 Query: 519 IGSNSTSNASSAITIGENSKVETGAENSIAIGKEAKSLKKDSIVLGTKAEAQGEGSIVLG 578 +T+ S+ + N+ + + + + I K + + EA + VL Sbjct: 220 KTQENTNKRSAELLANANAYADNKSSSVLGIANNYTDSKSAETLENARKEAFAQSKDVLN 279 Query: 579 Y---HSKSKENAISIGKESEALSIGSVSL----GHSSKAKGERSVSIGAYATAEKSNDI 630 HS S E A S+ +L H++K E S YA ++ S+ + Sbjct: 280 MAKAHSNSVARTTLETAEEHANSVARTTLETAEEHANKKSAEALASANVYADSKSSHTL 338 Score = 41.4 bits (96), Expect = 2e-05 Identities = 65/285 (22%), Positives = 115/285 (40%), Gaps = 17/285 (5%) Query: 410 SAGAIGDGSITAGAQ-------AKADGDNAIAIGKKAETKQSSGIAIGHSSKSEGNHSIS 462 +A A+G GSI G +KA GD+A+ G A T Q G+AIG + S + ++ Sbjct: 85 AAVAVGAGSIATGVNSVAIGPLSKALGDSAVTYG-AASTAQKDGVAIG-ARASTSDTGVA 142 Query: 463 IGKDSKASDLDSVALGHQARSTGSR--STALGPHSEATKDNALALGVWSKATMQGAIAIG 520 +G +SKA +SVA+GH + + S A+G S+ ++N++++G S +A G Sbjct: 143 VGFNSKADAKNSVAIGHSSHVAANHGYSIAIGDRSKTDRENSVSIGHESLNRQLTHLAAG 202 Query: 521 SNSTSNASSAITIGENSKVETGAENSIAIGKEAKSLKKDSIVLGTKAEAQGEGSIVLGYH 580 + T + A E K + K + L ++ + G Sbjct: 203 TKDTDAVNVAQLKKEIEKTQENTN------KRSAELLANANAYADNKSSSVLGIANNYTD 256 Query: 581 SKSKENAISIGKESEALSIGSVSLGHSSKAKGERSVSIGAYATAEKSNDIAIGLSSKASG 640 SKS E + KE+ A S +++ + R+ A A + + + + Sbjct: 257 SKSAETLENARKEAFAQSKDVLNMAKAHSNSVARTTLETAEEHANSVARTTLETAEEHAN 316 Query: 641 GYSIAIGLSAKAEASSSTAIGINSKADIEDSVALGSESKTTKATS 685 S SA A S ++ + + D S K + ++ Sbjct: 317 KKSAEALASANVYADSKSSHTLKTANSYTDVTVSNSTKKAIRESN 361 Score = 36.4 bits (83), Expect = 7e-04 Identities = 29/65 (44%), Positives = 39/65 (60%), Gaps = 3/65 (4%) Query: 341 VSIGDIEKGITRQITGLAAGKEDTDAVNVAQL-KSLDKKLEEENKTYFHVNTGKNKDTGD 399 VSIG + + RQ+T LAAG +DTDAVNVAQL K ++K E NK + N + Sbjct: 185 VSIG--HESLNRQLTHLAAGTKDTDAVNVAQLKKEIEKTQENTNKRSAELLANANAYADN 242 Query: 400 KDSNL 404 K S++ Sbjct: 243 KSSSV 247 Score = 34.1 bits (77), Expect = 0.004 Identities = 71/277 (25%), Positives = 108/277 (38%), Gaps = 31/277 (11%) Query: 857 MSKIDFEKSEVSIGKDGLNNGGKKITNVADGTESTDAVNKGQLDKLENKIDKTKKEIDKK 916 SK D E S VSIG + LN +++T++A GT+ TDAVN QL KKEI+K Sbjct: 176 RSKTDRENS-VSIGHESLN---RQLTHLAAGTKDTDAVNVAQL----------KKEIEKT 221 Query: 917 IEDIDKKVDKKIKDVEDKVDKKIEDTKKDLTDKIEKATKTLKTEITANNGEEANKTKGPV 976 E+ +K+ + + + D K + T + E N +EA V Sbjct: 222 QENTNKRSAELLANANAYADNK----SSSVLGIANNYTDSKSAETLENARKEAFAQSKDV 277 Query: 977 TLTSKKSDAGHNIYDISLATTQLKSSEGGKIETPNSEDSKKVANAGEVAKAINALGNNTL 1036 +K + A S +ET +KK A E + N ++ Sbjct: 278 LNMAKAHSNSVARTTLETAEEHANSVARTTLETAEEHANKKSA---EALASANVYADSKS 334 Query: 1037 SFGADKGNTEAQSLNKNGGLKFAIKGTDYIKTEAKGNEVSVDLTDKTKKDIEKGVSANSG 1096 S N+ N K + Y T+ K ++ L DK ++K G Sbjct: 335 SHTLKTANSYTDVTVSNSTKKAIRESNQY--TDHKFRQLDNRL-DKLDTRVDK------G 385 Query: 1097 VANAVAMANLPQINGKGH-NIAGSYGYYNGEHAFALG 1132 +A++ A+ +L Q G G N G Y A A+G Sbjct: 386 LASSAALNSLFQPYGVGKVNFTAGVGGYRSSQALAIG 422 Score = 33.7 bits (76), Expect = 0.004 Identities = 41/156 (26%), Positives = 65/156 (41%), Gaps = 4/156 (2%) Query: 24 VGANVLEGEKSLFVGLNSSTNNEEKVKIG--ANAKADSEKSIAIGMDSTSRGKKGIAIGA 81 +GA + + VG NS + + V IG ++ A+ SIAIG S + + ++IG Sbjct: 130 IGARASTSDTGVAVGFNSKADAKNSVAIGHSSHVAANHGYSIAIGDRSKTDRENSVSIGH 189 Query: 82 GSLAGAEKHLSNEIEDTIAIG-TDAKATATDAIAIGNKANARTKYGIAIGTETKTDGIAS 140 SL HL+ +DT A+ K NK +A + K+ + Sbjct: 190 ESLNRQLTHLAAGTKDTDAVNVAQLKKEIEKTQENTNKRSAELLANANAYADNKSSSVLG 249 Query: 141 IALGYKSDANSDSIAIG-KEAVGYGKGVALGENAHS 175 IA Y +++++ KEA K V AHS Sbjct: 250 IANNYTDSKSAETLENARKEAFAQSKDVLNMAKAHS 285
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Smon_0181	ARGDEIMINASE	487	e-174	Bacterial arginine deiminase signature.
>ARGDEIMINASE#Bacterial arginine deiminase signature. Length = 409 Score = 487 bits (1255), Expect = e-174 Identities = 190/403 (47%), Positives = 269/403 (66%), Gaps = 5/403 (1%) Query: 3 INVRSEINTLKKVLLHRPGKELLNLTPDTLERLLFDDVPFLKVAQAEHDRFAEILRENGV 62 IN+ SEI LKKVLLHRPG+EL NLTP ++ LFDD+P+L+VA+ EH+ FA IL+ N V Sbjct: 8 INIFSEIGRLKKVLLHRPGEELENLTPFIMKNFLFDDIPYLEVARQEHEVFASILKNNLV 67 Query: 63 EVVYLEDLVAETLDSSHELKVQFLKQFIEEGGVQLEVYKEALLNFFLSYTDTKEMVLKTM 122 E+ Y+EDL++E L SS L+ +F+ QFI E ++ + L ++F S M+ K + Sbjct: 68 EIEYIEDLISEVLVSSVALENKFISQFILEAEIKTDFTINLLKDYFSSL-TIDNMISKMI 126 Query: 123 EGVNMAELKVPRKDLVSYLDDPSELILDPMPNLYFTRDPFASTQNGVILNRMYSVTRNRE 182 GV ELK L ++ + I+DPMPN+ FTRDPFAS NGV +N+M++ R RE Sbjct: 127 SGVVTEELKNYTSSLDDLVNGANLFIIDPMPNVLFTRDPFASIGNGVTINKMFTKVRQRE 186 Query: 183 TIYAYYVFHYHPEYKGKVTFFYDRTNPFHIEGGDVLNINDKVLAIGISQRTEAAAIDLAA 242 TI+A Y+F YHP YK V + +R +EGGD L +N +L IGIS+RTEA +++ A Sbjct: 187 TIFAEYIFKYHPVYKENVPIWLNRWEEASLEGGDELVLNKGLLVIGISERTEAKSVEKLA 246 Query: 243 KTLLFDEANSHIETILAFRIAESRAWMHLDTVFTQIDHDKFSVHPAILGPLEVFELRRDG 302 +L + + +TILAF+I ++R++MHLDTVFTQID+ F+ + ++ L + Sbjct: 247 ISLFKN--KTSFDTILAFQIPKNRSYMHLDTVFTQIDYSVFTSFTSDDMYFSIYVLTYNP 304 Query: 303 NDVK--VTPKEGKLEDILAEYMGTKVTLIPCGGGDRIAAEREQWNDGSNTLCIAPGKVIV 360 + K + ++ +++D+L+ Y+G K+ +I C GGD I REQWNDG+N L IAPG++I Sbjct: 305 SSSKIHIKKEKARIKDVLSFYLGRKIDIIKCAGGDLIHGAREQWNDGANVLAIAPGEIIA 364 Query: 361 YERNDVTNDLLRKHGINVIEMPSAELSRGRGGPRCMSMPLVRE 403 Y RN VTN L ++GI V +PS+ELSRGRGGPRCMSMPL+RE Sbjct: 365 YSRNHVTNKLFEENGIKVHRIPSSELSRGRGGPRCMSMPLIRE 407
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Smon_0183	CARBMTKINASE	413	e-148	Bacterial carbamate kinase signature.
>CARBMTKINASE#Bacterial carbamate kinase signature. Length = 314 Score = 413 bits (1064), Expect = e-148 Identities = 155/313 (49%), Positives = 208/313 (66%), Gaps = 6/313 (1%) Query: 1 MGKRLVIALGGNALGN-----NPKEQLELVRGTAKAIVSMAKEGYEVIIGHGNGPQVGMI 55 MGKR+VIALGGNAL + +E ++ VR TA+ I + GYEV+I HGNGPQVG + Sbjct: 1 MGKRVVIALGGNALQQRGQKGSYEEMMDNVRKTARQIAEIIARGYEVVITHGNGPQVGSL 60 Query: 56 NLAMDYAANGEVKTPYMPFAECGAMSQGYIGYHLQQAIREELKTQNINKEVATIVTQVLV 115 L MD A P P GAMSQG+IGY +QQA++ EL+ + + K+V TI+TQ +V Sbjct: 61 LLHMD-AGQATYGIPAQPMDVAGAMSQGWIGYMIQQALKNELRKRGMEKKVVTIITQTIV 119 Query: 116 DKEDEAFKNLTKPIGMFYSKEVAEEIRKEKGFTFVEDAGRGYRRVVASPSPVKIIELNVV 175 DK D AF+N TKP+G FY +E A+ + +EKG+ ED+GRG+RRVV SP P +E + Sbjct: 120 DKNDPAFQNPTKPVGPFYDEETAKRLAREKGWIVKEDSGRGWRRVVPSPDPKGHVEAETI 179 Query: 176 KQLVEAGNIVITVGGGGIPVIETETGLKGVDAVIDKDKSSAKLAQDLNADMLVILTAVDK 235 K+LVE G IVI GGGG+PVI + +KGV+AVIDKD + KLA+++NAD+ +ILT V+ Sbjct: 180 KKLVERGVIVIASGGGGVPVILEDGEIKGVEAVIDKDLAGEKLAEEVNADIFMILTDVNG 239 Query: 236 VCINFNKPNQQELSELTLEEAVKYIEEGHFAKGSMLPKVEACLDFVKNSKGNALITSLEN 295 + + +Q L E+ +EE KY EEGHF GSM PKV A + F++ A+I LE Sbjct: 240 AALYYGTEKEQWLREVKVEELRKYYEEGHFKAGSMGPKVLAAIRFIEWGGERAIIAHLEK 299 Query: 296 AAIALQGKTGTLI 308 A AL+GKTGT + Sbjct: 300 AVEALEGKTGTQV 312
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Smon_0184	BINARYTOXINB	34	0.001	Binary toxin B family signature.
>BINARYTOXINB#Binary toxin B family signature. Length = 764 Score = 33.9 bits (77), Expect = 0.001 Identities = 14/55 (25%), Positives = 23/55 (41%), Gaps = 3/55 (5%) Query: 216 NYAKKIKSDKGSIILSLQEQQAMKDNFGHVDFSNLEFTTKHKMTLG---IFAFSF 267 +I+ II + ++ ++ V+ S+ TTK MTL AF F Sbjct: 508 EVLPQIQETTARIIFNGKDLNLVERRIAAVNPSDPLETTKPDMTLKEALKIAFGF 562
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Smon_0186	IGASERPTASE	119	8e-29	IgA-specific serine endopeptidase (S6) signature.
>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature. Length = 1541 Score = 119 bits (300), Expect = 8e-29 Identities = 125/559 (22%), Positives = 211/559 (37%), Gaps = 87/559 (15%) Query: 34 LARHDMNWEDYEDFAMNRGKYSIGREKVKVYKKDGTESGEI---SAPIPNFDGV-VDTGN 89 L R D++++ + DFA N+GK+S+G V V K+ + G P+ +F V VD Sbjct: 27 LVRDDVDYQIFRDFAENKGKFSVGATNVLVKDKNNKDLGTALPNGIPMIDFSVVDVDKRI 86 Query: 90 FALWGDSQILSGVHHVAPPKNFTF-------------SKRHFRNDVELFEGYKKLSLDDK 136 L ++ H F + R ++ + +K K Sbjct: 87 ATLINPQYVVGVKHVSNGVSELHFGNLNGNMNNGNAKAHRDVSSEENRYFSVEKNEYPTK 146 Query: 137 YTKFSESIEVAHRQKVEIDYALVRTDRIAFDAYVSEGIT--------KDQWKKIGIGDLV 188 + + E QK DY + R D+ + E T DQ K Sbjct: 147 LNGKTVTTE-DQTQKRREDYYMPRLDKFVTEVAPIEASTASSDAGTYNDQNKYPAF---- 201 Query: 189 ARVGRGLNRVAYDNGVEKDIDKDHHFAGGLNKITGRKKTGL------------------- 229 R+G G + I +H G K+ G T Sbjct: 202 VRLGSGSQFIYKKGDNYSLILNNHEVGGNNLKLVGDAYTYGIAGTPYKVNHENNGLIGFG 261 Query: 230 --NQDLQTSLEKTAKTPLDSGAKKGDSGSPLFWWDETNKKWLIAGSLSRGDAVGGYGKKL 287 ++ ++ PL + A GDSGSPLF +D KWL GS D GY KK Sbjct: 262 NSKEEHSDPKGILSQDPLTNYAVLGDSGSPLFVYDREKGKWLFLGSY---DFWAGYNKKS 318 Query: 288 YYLAHLSSYEDLKKSTTDKEITTETEGDVKFENGVLKV---GNEERKFKNKETISVNG-- 342 + + K T + ++ G + G +++++V+ Sbjct: 319 W-----QEWNIYKSQFTKDVLNKDSAGSLIGSKTDYSWSSNGKTSTITGGEKSLNVDLAD 373 Query: 343 ----NNTTKNQIFNKKGLEVKVEGNTNTYAARLEFKEDTTLKGSG---TLETAGFVVHKN 395 N K+ F G + + N + A L F+ D +KG+ T + AG V + Sbjct: 374 GKDKPNHGKSVTFEGSG-TLTLNNNIDQGAGGLFFEGDYEVKGTSDNTTWKGAGVSVAEG 432 Query: 396 KTLTYDISSNTSSTKGETKKITVRKVGEGKLVIKSTGKNIEHLNLGGGETVFENSIDNPV 455 KT+T+ + + + + K+G+G L+++ TG N L +G G + + + Sbjct: 433 KTVTWKVHN--------PQYDRLAKIGKGTLIVEGTGDNKGSLKVGDGTVILKQQTNGSG 484 Query: 456 A---DNIRLAQG-AKLTITKESQIKDSNVMFGHRGGTLNLNGTDLEFKDIYHMDKDAKIV 511 ++ + G + L + + Q+ +++ FG RGG L+LNG L F I ++D A++V Sbjct: 485 QHAFASVGIVSGRSTLVLNDDKQVDPNSIYFGFRGGRLDLNGNSLTFDHIRNIDDGARLV 544 Query: 512 NDKEGKDSKKSTFTFTPNS 530 N + S T T S Sbjct: 545 NHNM---TNASNITITGES 560 Score = 39.7 bits (92), Expect = 2e-04 Identities = 102/526 (19%), Positives = 172/526 (32%), Gaps = 89/526 (16%) Query: 878 NNNLEIDAKKGINLKINNNENNNSKIINLGYGASIDSKHKSLLKDN---SEGVLYLENDL 934 ++N + G +N + + N G + + L +N G L+ E D Sbjct: 352 SSNGKTSTITGGEKSLNVDLADGKDKPNHGKSVTFEGSGTLTLNNNIDQGAGGLFFEGDY 411 Query: 935 EETISNDKL-----AIGVAKSKEVTLNEDKSGNNKYYFSGEGKLGINHKLNNKELVVDGQ 989 E ++D + VA+ K VT ++ G+G L + +NK + G Sbjct: 412 EVKGTSDNTTWKGAGVSVAEGKTVTWKVHNPQYDRLAKIGKGTLIVEGTGDNKGSLKVGD 471 Query: 990 HFSGGVVELKQNSENYKGDVTVMGNKEGKNDGNITLKLGSDNALGKNNKVLLKDGGILDL 1049 G V LKQ + G G TL L D + N+ GG LDL Sbjct: 472 ----GTVILKQQTNGSGQHAF---ASVGIVSGRSTLVLNDDKQVDPNSIYFGFRGGRLDL 524 Query: 1050 NGKNLEAKIDENNNKHGSIINKN-EKYSTLKVSVEDKDLKFNNKISGNVNIVKKGNSAIE 1108 NG +L N + ++N N S + ++ E N N++ + N Sbjct: 525 NGNSLTFDHIRNIDDGARLVNHNMTNASNITITGESLITDPNTITPYNIDAPDEDNPYAF 584 Query: 1109 FTNEDNKFNEDKKANIYIKEGQLNYYNSKSLKNANVHIEDNTVLNTKTEQISSEITANGG 1168 +D GQL T + + Sbjct: 585 RRIKDG--------------GQLYLNL-----------------ENYTYYALRKGASTRS 613 Query: 1169 TIKVNSPTDKEKDSKATNFSKLTLKKDLVVEGTKSDDKSKVTYSEINLGGKKLTLKNQIV 1228 + NS E + + G SD+ + + IN N+ + Sbjct: 614 ELPKNSGESNE---------------NWLYMGKTSDEAKRNVMNHIN---------NERM 649 Query: 1229 ENADIY---ENGNNSGEVILENSTYYEEGARNEALYEKNVNEILYSKSVSKITLNNSDLV 1285 + Y E G N+G + N T+ + +N L N + +V K TL S Sbjct: 650 NGFNGYFGEEEGKNNGNL---NVTFKGKSEQNRFLLTGGTN-LNGDLTVEKGTLFLSGRP 705 Query: 1286 LKNYRSIGSYANTGQAVDIEVNGKSKITNLRQNDIVGTTNLKNTIDIKENASLTLGLNDK 1345 + R I ++T + N + + ++D + T+++ NASL G N Sbjct: 706 TPHARDIAGISSTKKDPHFAENNEV----VVEDDWINRNFKATTMNVTGNASLYSGRNVA 761 Query: 1346 NDNSSFVVNSKIKGKGKLILEQTNKGSITIKNNFKDFTGSIEANEN 1391 N + S I K + K T+ D+TG + + Sbjct: 762 N------ITSNITASNKAQVHIGYKTGDTV-CVRSDYTGYVTCTTD 800
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Smon_0189	TCRTETOQM	653	0.0	Tetracycline resistance protein TetO/TetQ/TetM family ...
>TCRTETOQM#Tetracycline resistance protein TetO/TetQ/TetM family signature. Length = 639 Score = 653 bits (1685), Expect = 0.0 Identities = 181/670 (27%), Positives = 307/670 (45%), Gaps = 66/670 (9%) Query: 10 TRNIGIMAHIDAGKTTTTERILFYTGVNHKLGEVHDGAATMDWMEQEQERGITITSAATT 69 NIG++AH+DAGKTT TE +L+ +G +LG V G D E++RGITI + T+ Sbjct: 3 IINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTDNTLLERQRGITIQTGITS 62 Query: 70 CFWKGHRINIIDTPGHVDFTVEVERSLRVLDGAVAVFSAVDGVQPQSETVWRQADKYNVP 129 W+ ++NIIDTPGH+DF EV RSL VLDGA+ + SA DGVQ Q+ ++ K +P Sbjct: 63 FQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAKDGVQAQTRILFHALRKMGIP 122 Query: 130 RLAFFNKMDRIGANFDMCVSDIKQKLGGNGVPIQLPIGAEDAFEGVIDLIEMKEYIFTDK 189 + F NK+D+ G + DIK+KL V Q K ++ + Sbjct: 123 TIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQ------------------KVELYPNM 164 Query: 190 QGEDYQVVDVRDTLKDDAELARNHLIESIVETDDELMEKYFAGEDISVEEIKRALRIATI 249 ++ + ++++E +D+L+EKY +G+ + E+++ I Sbjct: 165 CVTNFTESEQ---------------WDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFH 209 Query: 250 AGTVVPVCCGTAFKNKGIQPLLDAIVAYMPSPVDIEAVKGVDPKTELEISRKPADEEKFS 309 ++ PV G+A N GI L++ I S + + Sbjct: 210 NCSLFPVYHGSAKNNIGIDNLIEVITNKFYSSTH-------------------RGQSELC 250 Query: 310 ALAFKIVTDPFVGRLSFFRVYSGVLEKGSYVLNSTKGKKERMGRLLQMHANKREEIDVVY 369 FKI RL++ R+YSGVL V S K K ++ + + +ID Y Sbjct: 251 GKVFKIEYSEKRQRLAYIRLYSGVLHLRDSVRISEKEKI-KITEMYTSINGELCKIDKAY 309 Query: 370 AGDIAAAVG----LKDTTTGDTLCAEEAPIILERMEFPEPVISVAVEPKTKADQEKMGTA 425 +G+I L GDT + ER+E P P++ VEP +E + A Sbjct: 310 SGEIVILQNEFLKLNS-VLGDTKLLPQR----ERIENPLPLLQTTVEPSKPQQREMLLDA 364 Query: 426 LSKLAEEDPTFQVKSDQETGQTIIAGMGELHLEIIVDRMKREFKVEANVGKPQVAYRETI 485 L ++++ DP + D T + I++ +G++ +E+ ++ ++ VE + +P V Y E Sbjct: 365 LLEISDSDPLLRYYVDSATHEIILSFLGKVQMEVTCALLQEKYHVEIEIKEPTVIYMERP 424 Query: 486 LGSSDVEEKYAKQSGGRGQYGHVKIRVEAND-GKGYEFINEITGGAIPREYIPAVDKGVK 544 L + E + + + + V G G ++ + ++ G + + + AV +G++ Sbjct: 425 LKKA--EYTIHIEVPPNPFWASIGLSVSPLPLGSGMQYESSVSLGYLNQSFQNAVMEGIR 482 Query: 545 EALDSGVLAGYPVQDVKVTLYDGSYHEVDSSEMAFKIAGSMAMKKALRAANPILLEPIFK 604 + G L G+ V D K+ G Y+ S+ F++ + +++ L+ A LLEP Sbjct: 483 YGCEQG-LYGWNVTDCKICFKYGLYYSPVSTPADFRMLAPIVLEQVLKKAGTELLEPYLS 541 Query: 605 LEITTPEEYMGDVIGDLNSRRGTVSGMNDRNNAKIISGGVPLSEMFGYATDLRSKTQGRA 664 +I P+EY+ D + +NN I+SG +P + Y +DL T GR+ Sbjct: 542 FKIYAPQEYLSRAYTDAPKYCANIVDTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRS 601 Query: 665 TYSMEFEKYQ 674 E + Y Sbjct: 602 VCLTELKGYH 611
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Smon_0190	TCRTETOQM	83	2e-19	Tetracycline resistance protein TetO/TetQ/TetM family ...
>TCRTETOQM#Tetracycline resistance protein TetO/TetQ/TetM family signature. Length = 639 Score = 83.4 bits (206), Expect = 2e-19 Identities = 52/150 (34%), Positives = 79/150 (52%), Gaps = 7/150 (4%) Query: 13 VNVGTIGHVDHGKTTTTAAI---SKVLASKGLAQKVDFENIDQAPEERERGITINTAHIE 69 +N+G + HVD GKTT T ++ S + G K D ER+RGITI T Sbjct: 4 INIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGT-TRTDNTLLERQRGITIQTGITS 62 Query: 70 YESEARHYAHVDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQVGVP 129 ++ E +D PGH D++ + + +DGAIL++SA DG QTR R++G+P Sbjct: 63 FQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAKDGVQAQTRILFHALRKMGIP 122 Query: 130 YIVVYLNKVDMVEEEELLELVEMEVRELLS 159 I ++NK+D + L V +++E LS Sbjct: 123 TI-FFINKIDQNGID--LSTVYQDIKEKLS 149

Smon_0212

Smon_0222

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
Smon_0212	5	13	2.389914	transcriptional regulator, TetR family
Smon_0213	5	12	2.779346	ribosomal protein S2
Smon_0214	3	11	2.092035	translation elongation factor Ts
Smon_0215	1	11	0.884345	uridylate kinase
Smon_0216	1	11	-0.467839	ribosome recycling factor
Smon_0217	2	10	-0.052447	cell shape determining protein MreB/Mrl
Smon_0218	1	11	0.107763	transposase IS200-family protein
Smon_0219	0	11	0.654570	TPR repeat-containing protein
Smon_0220	2	10	0.752562	hypothetical protein
Smon_0221	1	10	0.043853	putative PTS IIA-like nitrogen-regulatory
Smon_0222	0	11	2.431553	periplasmic solute binding protein

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Smon_0212	HTHTETR	40	2e-06	TetR bacterial regulatory protein HTH signature.
>HTHTETR#TetR bacterial regulatory protein HTH signature. Length = 215 Score = 39.6 bits (92), Expect = 2e-06 Identities = 12/64 (18%), Positives = 29/64 (45%) Query: 2 RRKIKAKDLIRNAFAKTLKVKPYYRITVKELTEETGVTRQIFYYYFKNMTELLKYYFEVE 61 + + + I + + + ++ E+ + GVTR Y++FK+ ++L +E+ Sbjct: 7 QEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSEIWELS 66 Query: 62 IQEI 65 I Sbjct: 67 ESNI 70
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Smon_0215	CARBMTKINASE	30	0.008	Bacterial carbamate kinase signature.
>CARBMTKINASE#Bacterial carbamate kinase signature. Length = 314 Score = 29.8 bits (67), Expect = 0.008 Identities = 45/239 (18%), Positives = 76/239 (31%), Gaps = 76/239 (31%) Query: 5 KRILLKLSGEALAGDKEFGFSDDI---LHSFAKQIKEIHDEGVELAIVIGG----GNIFR 57 KR+++ L G AL + G +++ + A+QI EI G E+ I G G++ Sbjct: 3 KRVVIALGGNALQQRGQKGSYEEMMDNVRKTARQIAEIIARGYEVVITHGNGPQVGSLLL 62 Query: 58 -------GKFGEEVGMDRSTGDTMG----MLATIMNGLALQNAIEK-IGGVSTRVLTAIN 105 MD + + G M+ + + +EK + + T+ + N Sbjct: 63 HMDAGQATYGIPAQPMDVAGAMSQGWIGYMIQQALKNELRKRGMEKKVVTIITQTIVDKN 122 Query: 106 MPQVAEP-------------------------------------------FIRRRAIRHL 122 P P + I+ L Sbjct: 123 DPAFQNPTKPVGPFYDEETAKRLAREKGWIVKEDSGRGWRRVVPSPDPKGHVEAETIKKL 182 Query: 123 -EKGRVVIFAGGTGNPYFTTDSG-------------GALRAIEIEANVLAKGTKVDGIY 167 E+G +VI +GG G P D G A E+ A++ T V+G Sbjct: 183 VERGVIVIASGGGGVPVILEDGEIKGVEAVIDKDLAGEKLAEEVNADIFMILTDVNGAA 241
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Smon_0217	SHAPEPROTEIN	339	e-118	Bacterial cell shape determinant MreB/Mbl protein s...
>SHAPEPROTEIN#Bacterial cell shape determinant MreB/Mbl protein signature. Length = 347 Score = 339 bits (870), Expect = e-118 Identities = 145/351 (41%), Positives = 222/351 (63%), Gaps = 13/351 (3%) Query: 2 IFNKIINFFRIKKQISIDLGTSNVLFYDKQAKKIVLNEPSVIVKDKK----TDRVVAVGR 57 + K F +SIDLGT+N L Y K + IVLNEPSV+ + V AVG Sbjct: 1 MLKKFRGMFS--NDLSIDLGTANTLIYVK-GQGIVLNEPSVVAIRQDRAGSPKSVAAVGH 57 Query: 58 EAREMLGKNPKSIEVIKPLKDGVISDIDLTRKMLSEFMRQVYGISPF--KPEVIICVPIE 115 +A++MLG+ P +I I+P+KDGVI+D +T KML F++QV+ S P V++CVP+ Sbjct: 58 DAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNSFMRPSPRVLVCVPVG 117 Query: 116 VTKVERRALFDALDDV--KRIFLIEEGRAAIMGAGINISNPNGHMVIDIGGGSTDVAILS 173 T+VERRA+ ++ + +FLIEE AA +GAG+ +S G MV+DIGGG+T+VA++S Sbjct: 118 ATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATGSMVVDIGGGTTEVAVIS 177 Query: 174 LDEIIVSKSIKIAGNKFDEDIVKYVKEKLFLNIGDRTAEKIKKELSTAIFLPEEENKKMT 233 L+ ++ S S++I G++FDE I+ YV+ IG+ TAE+IK E+ +A P +E +++ Sbjct: 178 LNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERIKHEIGSA--YPGDEVREIE 235 Query: 234 IKGLDINTKKPKELVITSNQVCEAIEDSLNNLVAAVKEVIGKCPPELASDILDNGIVLTG 293 ++G ++ P+ + SN++ EA+++ L +V+AV + +CPPELASDI + G+VLTG Sbjct: 236 VRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALEQCPPELASDISERGMVLTG 295 Query: 294 GGALISNLYKLIENEVKVNVHVPDKPLDSVAIGGSYAFDNKNLLNTLLVKE 344 GGAL+ NL +L+ E + V V + PL VA GG A + ++ L E Sbjct: 296 GGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALEMIDMHGGDLFSE 346
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Smon_0219	SYCDCHAPRONE	34	2e-04	Gram-negative bacterial type III secretion SycD cha...
>SYCDCHAPRONE#Gram-negative bacterial type III secretion SycD chaperone signature. Length = 168 Score = 33.7 bits (77), Expect = 2e-04 Identities = 20/142 (14%), Positives = 40/142 (28%), Gaps = 6/142 (4%) Query: 46 AYLENDKAIKLYEELSKYLPNDHEVEGYLGYLYYENSNLNEAEERLKNALYLSEKEPFLL 105 ++L+ I + E+S + Y++ +A + + L + Sbjct: 17 SFLKGGGTIAMLNEISSDTLEQLYSLAFN---QYQSGKYEDAHKVFQALCVLDHYDSRFF 73 Query: 106 FLLGNVYSRKGMLREAFDCYELAIFLDFDMYGAHIDFGRKYEHMGRHRRALKEFRAAYDI 165 LG G A Y +D G A A ++ Sbjct: 74 LGLGACRQAMGQYDLAIHSYSYGAIMDIKEPRFPFHAAECLLQKGELAEAESGLFLAQEL 133 Query: 166 ---DSRDEELLKKIEHVENRIK 184 + +EL ++ + IK Sbjct: 134 IADKTEFKELSTRVSSMLEAIK 155
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Smon_0222	adhesinb	266	1e-90	Adhesin B signature.
>adhesinb#Adhesin B signature. Length = 310 Score = 266 bits (681), Expect = 1e-90 Identities = 88/313 (28%), Positives = 162/313 (51%), Gaps = 13/313 (4%) Query: 1 MKNLKQILLALMLTVFAFSCGSKMGDAKSDEGKIKVTTTLNYYVNLLEEIGKDKVKVTGL 60 MK + ++L L+ V +C S+ ++ K+ V T + ++ + I DK+ + + Sbjct: 1 MKKCRFLVLLLLAFVGLAACSSQKSSTETGSSKLNVVATNSIIADITKNIAGDKINLHSI 60 Query: 61 MGEGEDPHLYVATAGDIEKLEKADLVVYGGLHLEGKMVEIFENL-------KDKAVLDLG 113 + G+DPH Y D++K +ADL+ Y G++LE F L ++K + Sbjct: 61 VPVGQDPHEYEPLPEDVKKTSQADLIFYNGINLETGGNAWFTKLVENAKKKENKDYYAVS 120 Query: 114 AQLDPSKLV-EEEKGVYDPHVWFNTEFWAVQATAVANKLSELDPANKEFYMNNLEVYLKE 172 +D L + EKG DPH W N E + A +A +LSE DPANKE Y NL+ Y+++ Sbjct: 121 EGVDVIYLEGQSEKGKEDPHAWLNLENGIIYAQNIAKRLSEKDPANKETYEKNLKAYVEK 180 Query: 173 LDMATKYVQDKINEIPENARVLITAHDAFGYFASQFGLEVKAIQGVSTDSEIGTKEINEL 232 L K ++K N IP ++++T+ F YF+ + + I ++T+ E +I L Sbjct: 181 LSALDKEAKEKFNNIPGEKKMIVTSEGCFKYFSKAYNVPSAYIWEINTEEEGTPDQIKTL 240 Query: 233 ADFIVANKIKAIFVESSVNHKSIESLQEAVQAKGFEVKIGGELYSDSMGDAKNNTETYIK 292 + + K+ ++FVESSV+ + ++++ +K + I ++++DS+ + ++Y Sbjct: 241 VEKLRKTKVPSLFVESSVDDRPMKTV-----SKDTNIPIYAKIFTDSVAEKGEEGDSYYS 295 Query: 293 TLKFNADTIANAL 305 +K+N + IA L Sbjct: 296 MMKYNLEKIAEGL 308

Smon_0237

Smon_0240

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
Smon_0237	1	16	1.432231	OmpA/MotB domain protein
Smon_0238	3	17	-1.851835	putative transcriptional acitvator, Baf family
Smon_0239	1	19	-2.763837	hypothetical protein
Smon_0240	1	16	-4.202305	hypothetical protein

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Smon_0237	OMPADOMAIN	105	2e-27	OMPA domain signature.
>OMPADOMAIN#OMPA domain signature. Length = 346 Score = 105 bits (264), Expect = 2e-27 Identities = 49/148 (33%), Positives = 75/148 (50%), Gaps = 8/148 (5%) Query: 342 EVVKEVEVPVHIKPQI--KKIELSADALFKFDKYKLEDMLEKGKMEIQELVKKLSTDYVR 399 E V P++ K L +D LF F+K L+ +G+ + +L +LS + Sbjct: 195 EAAPVVAPAPAPAPEVQTKHFTLKSDVLFNFNKATLK---PEGQAALDQLYSQLSNLDPK 251 Query: 400 LDRIDIIGHTDRLGSDSYNLALGLRRAQTVRSYLQELGVTTP-ITVASKGKRDPKV--KC 456 + ++G+TDR+GSD+YN L RRAQ+V YL G+ I+ G+ +P C Sbjct: 252 DGSVVVLGYTDRIGSDAYNQGLSERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTC 311 Query: 457 PGTKATAKLKQCLLPNRRVEINLTGLEV 484 K A L CL P+RRVEI + G++ Sbjct: 312 DNVKQRAALIDCLAPDRRVEIEVKGIKD 339
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Smon_0238	PF03309	194	1e-63	Bvg accessory factor
>PF03309#Bvg accessory factor Length = 271 Score = 194 bits (495), Expect = 1e-63 Identities = 62/246 (25%), Positives = 117/246 (47%), Gaps = 16/246 (6%) Query: 1 MILGFDIGNTHICPIIYDNN---GKILEKFRIPSKTNLTEDTLYATLKTLCDFKKIDLSD 57 M+L D+ NTH + + K+++++RI ++ +T D L T+ L D Sbjct: 1 MLLAIDVRNTHTVVGLISGSGDHAKVVQQWRIRTEPEVTADELALTIDGLI---GDDAER 57 Query: 58 VKDVVYSSVVPHLNNVFDYLAKKYFNCEPYVLNINNIDENLLTFNANTERNLGADRIA-T 116 + S VP + + + ++Y+ P+VL + + + + +GADRI Sbjct: 58 LTGASGLSTVPSVLHEVRVMLEQYWPNVPHVLIEPGVRTGI-PLLVDNPKEVGADRIVNC 116 Query: 117 ILAMKKYMSNKKCIIIDFGTATTFEVI-KDNKYLGGAILPGIDLSINALFQNTAKLPKVT 175 + A KY + I++DFG++ +V+ ++LGGAI PG+ +S +A +A L +V Sbjct: 117 LAAYHKYGTA--AIVVDFGSSICVDVVSAKGEFLGGAIAPGVQVSSDAAAARSAALRRVE 174 Query: 176 FEKPNEVLGNTTVTQINIGIYYSNIGAIKELINQYKNIYP-----DAYVISTGGQGKIIT 230 +P V+G TV + G + G + L+N+ ++ D V++TG ++ Sbjct: 175 LTRPRSVIGKNTVECMQAGAVFGFAGLVDGLVNRIRDDVDGFSGADVAVVATGHTAPLVL 234 Query: 231 EDLKDF 236 DL+ Sbjct: 235 PDLRTV 240
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Smon_0239	BCTERIALGSPG	50	7e-11	Bacterial general secretion pathway protein G signa...
>BCTERIALGSPG#Bacterial general secretion pathway protein G signature. Length = 145 Score = 50.3 bits (120), Expect = 7e-11 Identities = 25/58 (43%), Positives = 36/58 (62%) Query: 11 KKNRGFTLIEIITVIAIIGILASISVPKISKYIDRANETKIFSAVSELNNLYILMNLD 68 K RGFTL+EI+ VI IIG+LAS+ VP + ++A++ K S + L N + LD Sbjct: 5 DKQRGFTLLEIMVVIVIIGVLASLVVPNLMGNKEKADKQKAVSDIVALENALDMYKLD 62
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Smon_0240	PREPILNPTASE	33	3e-04	Type IV prepilin cysteine protease (C20) family sig...
>PREPILNPTASE#Type IV prepilin cysteine protease (C20) family signature. Length = 290 Score = 32.9 bits (75), Expect = 3e-04 Identities = 28/136 (20%), Positives = 53/136 (38%), Gaps = 15/136 (11%) Query: 6 YLMLLYISYVDFVEGYIYDR-DLVILFIFLYFSTTSGIYSSYVGMGIFSLPFFILWILES 64 +L+ ++++D + + D+ L +L+ L F+ G S + + +LW L Sbjct: 141 TWVLVALTFIDLDKMLLPDQLTLPLLWGGLLFNLLGGFVSLGDAVIGAMAGYLVLWSLYW 200 Query: 65 YFNF----EIIGMGDIKLMLIFGMYFGIKDMHFIFTFYEIMYFSSLIYAIILR------- 113 F E +G GD KL+ G + G + + + + I L Sbjct: 201 AFKLLTGKEGMGYGDFKLLAALGAWLGWQALPIVLLLSSL---VGAFMGIGLILLRNHHQ 257 Query: 114 KKYVPFAPAMCFSFII 129 K +PF P + + I Sbjct: 258 SKPIPFGPYLAIAGWI 273

Smon_0630

Smon_0636

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
Smon_0630	-1	10	-1.275614	SpoIID/LytB domain protein
Smon_0631	0	10	-0.562826	tRNA(Ile)-lysidine synthetase
Smon_0632	0	10	-0.572837	ATP-dependent metalloprotease FtsH
Smon_0633	0	11	-2.097978	ribosomal protein L32
Smon_0634	0	10	-2.440691	MutS2 family protein
Smon_0635	-1	7	-2.554079	cysteinyl-tRNA synthetase
Smon_0636	-2	7	-1.033604	cell shape determining protein, MreB/Mrl family

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Smon_0630	SALSPVBPROT	30	0.017	Salmonella virulence plasmid 65kDa B protein signature.
>SALSPVBPROT#Salmonella virulence plasmid 65kDa B protein signature. Length = 591 Score = 30.1 bits (67), Expect = 0.017 Identities = 17/48 (35%), Positives = 27/48 (56%) Query: 209 IVHDNKIIDAVFHSYSGGYTASGKEVWGNDVPYLQAVEDNYSKDVNSS 256 IV ++K I A+ + + GY+ K + G+D P QA E S+D S+ Sbjct: 388 IVEESKQIQALRYYSAQGYSVINKYLRGDDYPETQAKETLLSRDYLST 435
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Smon_0632	HTHFIS	36	4e-04	FIS bacterial regulatory protein HTH signature.
>HTHFIS#FIS bacterial regulatory protein HTH signature. Length = 484 Score = 36.3 bits (84), Expect = 4e-04 Identities = 54/250 (21%), Positives = 82/250 (32%), Gaps = 71/250 (28%) Query: 260 ARVPKGVLLLGEPGTGKTLLAKAVAGES---EAAFFPIS---------GSEFIELYVGV- 306 + +++ GE GTGK L+A+A+ F I+ SE G Sbjct: 157 MQTDLTLMITGESGTGKELVARALHDYGKRRNGPFVAINMAAIPRDLIESELFGHEKGAF 216 Query: 307 -GASRVRE-LFKDAKKEAPAIIFIDEIDAVGR-------RRGQNKNGG--GGNEEREQTL 355 GA F+ A+ +F+DEI + R Q GG Sbjct: 217 TGAQTRSTGRFEQAEG---GTLFLDEIGDMPMDAQTRLLRVLQQGEYTTVGGRTPIR--- 270 Query: 356 NQLLVEMDGFDTDQRIIVMAATNRSDVLDP-ALLRGGRFDR----RIEVSR---PDVKGR 407 +D RI+ AATN+ D + G F R+ V P ++ R Sbjct: 271 -----------SDVRIV--AATNK----DLKQSINQGLFREDLYYRLNVVPLRLPPLRDR 313 Query: 408 IE--------ILKVHSRNKKLASDVKLEDIAKIT----PGFVGADLENLLNEAAILAARK 455 E ++ + E + + PG V +LENL+ L Sbjct: 314 AEDIPDLVRHFVQQAEKEGLDVKRFDQEALELMKAHPWPGNV-RELENLVRRLTALYP-- 370 Query: 456 NSDEITMEDL 465 D IT E + Sbjct: 371 -QDVITREII 379
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Smon_0634	BONTOXILYSIN	39	8e-05	Bontoxilysin signature.
>BONTOXILYSIN#Bontoxilysin signature. Length = 1196 Score = 39.1 bits (91), Expect = 8e-05 Identities = 25/172 (14%), Positives = 59/172 (34%), Gaps = 19/172 (11%) Query: 499 KEIIDNARSYISEDNREVEKMLASIKEKNDNLEAMNIEVEKLKQELANEKSNYENKIAEF 558 K+I+ N + +S+ + D L+ + EK +L+NE N++ F Sbjct: 699 KQIVQNKFTDLSKASIP-----------PDTLKLIRETTEKTFIDLSNESQISMNRVDNF 747 Query: 559 EKE------KNNILKDAYKKADDYIKDMQNKAKALVDKINSDNVKKEEAKTLQKNINMIR 612 + +I + YI ++ K + + + + N ++ I Sbjct: 748 LNKASICVFVEDIYPKFISYMEKYINNINIKTREFIQRCTNINDNEKSILINSYTFKTID 807 Query: 613 QYIEDSKKENIVEKKYTKSDLNFEINEEVLIKTLNQVGK--VLRIIPEKNSL 662 D + + + ++ L+ ++ ++ I KN+L Sbjct: 808 FKFLDIQSIKNFFNSQVEQVMKEILSPYQLLLFASKGPNSNIIEDISGKNTL 859
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Smon_0636	SHAPEPROTEIN	355	e-124	Bacterial cell shape determinant MreB/Mbl protein s...
>SHAPEPROTEIN#Bacterial cell shape determinant MreB/Mbl protein signature. Length = 347 Score = 355 bits (914), Expect = e-124 Identities = 149/328 (45%), Positives = 225/328 (68%), Gaps = 9/328 (2%) Query: 20 ISIDLGTANLLIYDKQEDKIVLNEPSVLARDRKTG----KVIAVGKEAREMLGKTPDSIE 75 +SIDLGTAN LIY K + IVLNEPSV+A + V AVG +A++MLG+TP +I Sbjct: 13 LSIDLGTANTLIYVKGQG-IVLNEPSVVAIRQDRAGSPKSVAAVGHDAKQMLGRTPGNIA 71 Query: 76 AIKPLKDGVIADLDATREMLSHFMYKIYGSSIF--KPEVMICVPLEVTPVERKALFDSV- 132 AI+P+KDGVIAD T +ML HF+ +++ +S P V++CVP+ T VER+A+ +S Sbjct: 72 AIRPMKDGVIADFFVTEKMLQHFIKQVHSNSFMRPSPRVLVCVPVGATQVERRAIRESAQ 131 Query: 133 -SGAKKIYIIEEGRAAIIGSGIDISKPAGNMVIDIGGGSTDVAILSLDEVIASKSIRIAG 191 +GA+++++IEE AA IG+G+ +S+ G+MV+DIGGG+T+VA++SL+ V+ S S+RI G Sbjct: 132 GAGAREVFLIEEPMAAAIGAGLPVSEATGSMVVDIGGGTTEVAVISLNGVVYSSSVRIGG 191 Query: 192 NKFDEDIIRYVRNKYNLLIGDRTAEKIKKELATAIYEEVPKVMSIKGRQLEVQTPVSIQI 251 ++FDE II YVR Y LIG+ TAE+IK E+ +A + + + ++GR L P + Sbjct: 192 DRFDEAIINYVRRNYGSLIGEATAERIKHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTL 251 Query: 252 DSNEVNEAIKSSLYSIINAVKEVLEKSPPELAADILDNGIVMTGGGSMIKNFTTLVEQEV 311 +SNE+ EA++ L I++AV LE+ PPELA+DI + G+V+TGGG++++N L+ +E Sbjct: 252 NSNEILEALQEPLTGIVSAVMVALEQCPPELASDISERGMVLTGGGALLRNLDRLLMEET 311 Query: 312 QVKVYLSEHPLDSVVLGGGKAFDNKKLL 339 + V ++E PL V GGGKA + + Sbjct: 312 GIPVVVAEDPLTCVARGGGKALEMIDMH 339

Smon_1379

Smon_1384

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
Smon_1379	3	8	1.478246	Peptidase M23
Smon_1380	0	10	-0.713778	Cell division protein-like protein
Smon_1381	1	9	-2.167578	Transketolase central region
Smon_1382	1	9	-3.321182	Transketolase domain protein
Smon_1383	1	9	-4.420778	major facilitator superfamily MFS_1
Smon_1384	0	9	-5.433923	two component transcriptional regulator, LuxR

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Smon_1379	GPOSANCHOR	34	9e-04	Gram-positive coccus surface protein anchor signature.
>GPOSANCHOR#Gram-positive coccus surface protein anchor signature. Length = 539 Score = 34.3 bits (78), Expect = 9e-04 Identities = 50/295 (16%), Positives = 114/295 (38%), Gaps = 22/295 (7%) Query: 13 SLASFGNNIDKNKNRINQIDKQVKDNTNKINNNNSKINNAKKDEAAIKKEIQELDALISK 72 + + R ++K ++ N +++KI + ++AA++ EL+ + Sbjct: 212 KIKTLEAEKAALAARKADLEKALEGAMNFSTADSAKIKTLEAEKAALEARQAELEKALEG 271 Query: 73 LQREYNVIQNEYVSLLKEIGKSEKEIRS--SIRKIEESSKKITEGKTDYSNKINTWNKVF 130 + +L E E E ++ ++++ D S + + Sbjct: 272 AMNFSTADSAKIKTLEAEKAALEAEKADLEHQSQVLNANRQSLRRDLDASREAKKQLEAE 331 Query: 131 NAKVFQKNSFSSESAKKTNDLIKVLE----QGQNNIKKIEKYKQQEEIHKKNEEILKNKT 186 + K+ ++N S S + + Q + +K+E+ + E +++ + + Sbjct: 332 HQKLEEQNKISEASRQSLRRDLDASREAKKQLEAEHQKLEEQNKISEASRQSLRRDLDAS 391 Query: 187 QKEAKKVEKKKLELENKREELRKAKINKDRAVKNLQNLQDSLKDENKKIERTNSSLIAEK 246 ++ K+VEK E +K L K + L++S K K+ + L AE Sbjct: 392 REAKKQVEKALEEANSKLAALEKL----------NKELEESKKLTEKEKAELQAKLEAEA 441 Query: 247 RRLEQQINAIIAAAKKREEDARKRAQGSNKNQSGNESTDKKEPIKAVVVPKGTGK 301 + L++++ AK+ EE A+ RA ++ +Q+ + K P+ K Sbjct: 442 KALKEKL------AKQAEELAKLRAGKASDSQTPDAKPGNKAVPGKGQAPQAGTK 490 Score = 31.6 bits (71), Expect = 0.006 Identities = 30/243 (12%), Positives = 79/243 (32%), Gaps = 17/243 (6%) Query: 23 KNKNRINQIDKQVKDNTNKINNNNSKINNAKKDEAAIKKEIQELDALISKLQREYNVIQN 82 + +I ++ + + + + A A +I+ L+A + L+ ++ Sbjct: 138 ADSAKIKTLEAEKAALAARKADLEKALEGAMNFSTADSAKIKTLEAEKAALEARQAELEK 197 Query: 83 EYVSLLKEIGKSE---KEIRSSIRKIEESSKKITEGKTDYSNKINTWNKVFNAKVFQKNS 139 + K + + + + + N + +K + Sbjct: 198 ALEGAMNFSTADSAKIKTLEAEKAALAARKADLEKALEGAMNFSTADSAKIKTLEAEKAA 257 Query: 140 FSSESA---KKTNDLIKVLEQGQNNIKKIEKYKQQ---------EEIHKKNEEILKNKTQ 187 + A K + IK +E K + N + Sbjct: 258 LEARQAELEKALEGAMNFSTADSAKIKTLEAEKAALEAEKADLEHQSQVLNANRQSLRRD 317 Query: 188 KEAKKVEKKKLELENKREELRKAKINKDRAVKNLQNLQDSLKDENKKIERTNSSLIAEKR 247 +A + KK+LE E++ +L + + + ++L+ D+ ++ K++E + L + + Sbjct: 318 LDASREAKKQLEAEHQ--KLEEQNKISEASRQSLRRDLDASREAKKQLEAEHQKLEEQNK 375 Query: 248 RLE 250 E Sbjct: 376 ISE 378
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Smon_1380	TYPE3IMSPROT	29	0.020	Type III secretion system inner membrane S protein ...
>TYPE3IMSPROT#Type III secretion system inner membrane S protein family signature. Length = 354 Score = 29.3 bits (66), Expect = 0.020 Identities = 16/97 (16%), Positives = 37/97 (38%), Gaps = 9/97 (9%) Query: 211 FLESVLAVLISGAVSYIMYVKIRCSIVGLIEKARGATAIQGFSTLGKETNFLFIVLIITI 270 FL+S+L V++ + +I+ +++ L G + + L++ Sbjct: 140 FLKSILKVVLLSILIWIIIKGNLVTLLQL--------PTCGIECITPLLGQILRQLMVIC 191 Query: 271 ILVLVINYLFLNKYYNIRYYEKNKNEEIQEVKEEVEE 307 + V+ + + + Y K E+K E +E Sbjct: 192 TVGFVVISIA-DYAFEYYQYIKELKMSKDEIKREYKE 227
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Smon_1383	TCRTETA	36	3e-04	Tetracycline resistance protein signature.
>TCRTETA#Tetracycline resistance protein signature. Length = 399 Score = 35.6 bits (82), Expect = 3e-04 Identities = 58/353 (16%), Positives = 119/353 (33%), Gaps = 43/353 (12%) Query: 34 YLPSEKAIADYGISVIMPPVYLAIAFVIMRFVDAIADPVVGYMSDNSTSSLGRRSFYMLI 93 + S A YG L + +M+F PV+G +SD GRR + Sbjct: 35 LVHSNDVTAHYG--------ILLALYALMQF---ACAPVLGALSD----RFGRR----PV 75 Query: 94 GIIPLALSMIAFFFPFNNGPLIATLYLAFVGSIYFIAYTLVGGPYNALIPDLASNKEERL 153 ++ LA + + + P + LY+ G I G A I D+ E Sbjct: 76 LLVSLAGAAVDYAI-MATAPFLWVLYI---GRIVAGITGATGAVAGAYIADITDGDERAR 131 Query: 154 NLSTIQSVFRLIFTAIPLIFSPIILSNMIKSGMSFIKAMRLMVTGFSVLSAIIVIISVML 213 + + + F A P++ L F A + L+ + + L Sbjct: 132 HFGFMSACFGFGMVAGPVLGG---LMGGFSPHAPFFAA--------AALNGLNFLTGCFL 180 Query: 214 LKENKVRHNNNNDVKKINFKEAFSYLKNKEIILYFIGFFFFFSGFNIIRNSV-LYYVTVI 272 L E+ + +N +F + + ++ + FF + ++ + + Sbjct: 181 LPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIMQLVGQVPAALWVIFGEDR 240 Query: 273 LGQSEKAASLPTTILFAMSALF-FPVTQFLSKKFDYRKVMLFDLALIILGTLGLIFLGDK 331 + + +L +T ++ + R+ ++ + G + L F Sbjct: 241 FHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALMLGMIADGTGYILLAFATR- 299 Query: 332 SKSIFFAMFLVVGAGVSGSAFIFPPAMLSEISIKLHEKYNVSVEGMMFGIQGL 384 F M L+ G I PA+ + +S ++ E+ ++G + + L Sbjct: 300 GWMAFPIMVLLASGG------IGMPALQAMLSRQVDEERQGQLQGSLAALTSL 346
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Smon_1384	HTHFIS	48	6e-09	FIS bacterial regulatory protein HTH signature.
>HTHFIS#FIS bacterial regulatory protein HTH signature. Length = 484 Score = 47.9 bits (114), Expect = 6e-09 Identities = 21/117 (17%), Positives = 49/117 (41%), Gaps = 6/117 (5%) Query: 2 KILLIDDHKLFALSIQMILGKYKEIERIDVITDSKQ-LEKKDIKDYDIYLIDINLNNISE 60 IL+ DD + L + + + +++ D D+ + D+ + + Sbjct: 5 TILVADDDAAIRTVLNQALSR--AGYDVRITSNAATLWRWIAAGDGDLVVTDVVM---PD 59 Query: 61 ETGLELAEKLIKKDKNICIVILTGHLKLMYEDKANKIGVRGFIDKNIDPEELIKILK 117 E +L ++ K ++ +++++ M KA++ G ++ K D ELI I+ Sbjct: 60 ENAFDLLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIG 116

Smon_1478

Smon_1495

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
Smon_1478	2	12	2.584836	hypothetical protein
Smon_1479	3	12	5.024545	ribosomal protein S18
Smon_1480	2	13	4.652288	single-strand binding protein
Smon_1481	0	12	4.354723	ribosomal protein S6
Smon_1482	0	12	4.065876	YadA domain protein
Smon_1483	0	12	3.685301	YadA domain protein
Smon_1485	-2	9	2.863188	YadA domain protein
Smon_1486	-2	8	1.861572	YadA domain protein
Smon_1487	-3	7	1.005387	YadA domain protein
Smon_1488	1	12	-4.408664	rhoptry protein
Smon_1489	1	13	-2.827257	Propeptide PepSY amd peptidase M4
Smon_1490	2	14	-3.241892	two component transcriptional regulator, winged
Smon_1491	3	14	-3.754309	histidine kinase
Smon_1492	1	16	0.016665	hypothetical protein
Smon_1493	-1	16	0.680909	hypothetical protein
Smon_1495	0	14	2.446470	hypothetical protein

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Smon_1478	CHANLCOLICIN	42	5e-06	Channel forming colicin signature.
>CHANLCOLICIN#Channel forming colicin signature. Length = 522 Score = 42.4 bits (99), Expect = 5e-06 Identities = 33/187 (17%), Positives = 72/187 (38%), Gaps = 15/187 (8%) Query: 381 NAYEGEKQRIKNMLENQTEDDKKKREEELKKLKENIDKKQKEIDDYKKKKD-FTSEIENA 439 A K E Q + E + ++ +I + QK I ++ + + A Sbjct: 272 EATRRRVGAGKIREEKQKQV--TASETRINRINADITQIQKAISQVSNNRNAGIARVHEA 329 Query: 440 LEKQKSRRDDLEDRKKEIKDEIKEKT--YDKIEEYFKEKLEELAKLEKENNISSKNYSSF 497 E K +++L +IKD + Y + E + EK ++A+ E + K + Sbjct: 330 EENLKKAQNNLL--NSQIKDAVDATVSFYQTLTEKYGEKYSKMAQ-ELADKSKGKKIGNV 386 Query: 498 --LMVSREKTREKRKEKIKE-----ISDKLNINDKEKFAEKYEKLLEYRGNGLYASVKSK 550 + + EK ++ +K + I + L + +A+ ++ +Y + S Sbjct: 387 NEALAAFEKYKDVLNKKFSKADRDAIFNALASVKYDDWAKHLDQFAKYLKITGHVSFGYD 446 Query: 551 ILIDLTK 557 ++ D+ K Sbjct: 447 VVSDILK 453
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Smon_1482	OMADHESIN	57	9e-11	Yersinia outer membrane adhesin signature.
>OMADHESIN#Yersinia outer membrane adhesin signature. Length = 455 Score = 57.2 bits (137), Expect = 9e-11 Identities = 72/338 (21%), Positives = 138/338 (40%), Gaps = 16/338 (4%) Query: 23 SSSTNPTLGNGSKANGESSLALGINSNAEGKHDVAIGTNAQTNKGGNKGSNGGAVAIGSN 82 S + +P LG A G+N++A+G H +AIG A+ KG G++A G N Sbjct: 40 SPNADPALGLEYPVRPPVPGAGGLNASAKGIHSIAIGATAEAAKGAAVAVGAGSIATGVN 99 Query: 83 -------SKADGDNAFAIGNGAIAEKSSSMSFGGKASGTGATNIGYSGESTGDSAVSIGY 135 SKA GD+A G + A+K ++ +G++ ++ ++V+IG+ Sbjct: 100 SVAIGPLSKALGDSAVTYGAASTAQKDGVAIGARASTSDTGVAVGFNSKADAKNSVAIGH 159 Query: 136 SAK--SKESYATAIGGQSEASKTLSTAIGSASKAKNQSSTAIGASSEASYDNAIAIGASS 193 S+ + Y+ AIG +S+ + S +IG S + + A G + + A Sbjct: 160 SSHVAANHGYSIAIGDRSKTDRENSVSIGHESLNRQLTHLAAGTKDTDAVNVAQLKKEIE 219 Query: 194 KTDSKATQESSAKIGDFTYSGFSGEAGTDGHQVSVGAKDSERQIKNVASGKVSKDSTDAV 253 KT + S+ + + + + G + S ++N A + S D + Sbjct: 220 KTQENTNKRSAELLANANAYADNKSSSVLGIANNYTDSKSAETLEN-ARKEAFAQSKDVL 278 Query: 254 NGSQLFSTSKALENLANSTKKILGGNSKITKTGDNIAN------IDMTNIGGTGKNNIDD 307 N ++ S S A L + + +T + AN + N+ K++ Sbjct: 279 NMAKAHSNSVARTTLETAEEHANSVARTTLETAEEHANKKSAEALASANVYADSKSSHTL 338 Query: 308 AIKAAITEVKSENNSSIIVKSTTGANGNKVYTLDTKID 345 + T+V N++ ++ + +K LD ++D Sbjct: 339 KTANSYTDVTVSNSTKKAIRESNQYTDHKFRQLDNRLD 376 Score = 33.3 bits (75), Expect = 0.002 Identities = 25/97 (25%), Positives = 53/97 (54%), Gaps = 5/97 (5%) Query: 14 SIISYADTSSSSTNPTLGNGSKANGESSLALGINSNAEGKHDVAIGTNAQTNKGGNKGSN 73 S ++Y S++ + + ++ +A+G NS A+ K+ VAIG ++ ++ Sbjct: 113 SAVTYGAASTAQKDGVAIGARASTSDTGVAVGFNSKADAKNSVAIGHSSHV-----AANH 167 Query: 74 GGAVAIGSNSKADGDNAFAIGNGAIAEKSSSMSFGGK 110 G ++AIG SK D +N+ +IG+ ++ + + ++ G K Sbjct: 168 GYSIAIGDRSKTDRENSVSIGHESLNRQLTHLAAGTK 204
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Smon_1483	OMADHESIN	64	2e-12	Yersinia outer membrane adhesin signature.
>OMADHESIN#Yersinia outer membrane adhesin signature. Length = 455 Score = 64.1 bits (155), Expect = 2e-12 Identities = 91/331 (27%), Positives = 146/331 (44%), Gaps = 62/331 (18%) Query: 116 GSHSSSKGAVFGVGIGFQARATKASAIAIGAGSYAEGYYSQAYGRSATALGNESIAQGVT 175 G ++S+KG + + IG A A K +A+A+GAGS A G S A G + ALG+ ++ G Sbjct: 62 GLNASAKG-IHSIAIGATAEAAKGAAVAVGAGSIATGVNSVAIGPLSKALGDSAVTYGAA 120 Query: 176 ALAKEKGSIAIGTNATSSGENAIAIGSSARERNALYQTDSKKRVDASGKNSVALGHIAQA 235 + A++ G +AIG A++S + +A+G +++ A KNSVA+GH + Sbjct: 121 STAQKDG-VAIGARASTS-DTGVAVGFNSK---------------ADAKNSVAIGHSSHV 163 Query: 236 SGENALSFGYKAESTMMGAIALGSESKTTVDKGVMGYVPSADKTLSGNAWQSTHAALAIG 295 + + S IA+G SKT + V +IG Sbjct: 164 AANHGYS------------IAIGDRSKTDRENSV-----------------------SIG 188 Query: 296 NGSTVTRQITGLAAGKEDTDAVNVAQLKALATKLEDDKKTYFHVNTGTHNGGNKSSNLGK 355 + S + RQ+T LAAG +DTDAVNVAQLK K +++ N + + Sbjct: 189 HES-LNRQLTHLAAGTKDTDAVNVAQLKKEIEKTQENTNKRSAELLANANAYADNKSSSV 247 Query: 356 IGEAAGAEGKYSITAGMNAN------AKEEASIAIGYNSKSLKKHTIAVGMHANSEGEAS 409 +G A S NA +K+ ++A +++ + HANS + Sbjct: 248 LGIANNYTDSKSAETLENARKEAFAQSKDVLNMAKAHSNSVARTTLETAEEHANSVARTT 307 Query: 410 ISIG--YGAKTSNNYSISLGRYAESKGTDSL 438 + + K S S YA+SK + +L Sbjct: 308 LETAEEHANKKSAEALASANVYADSKSSHTL 338 Score = 57.2 bits (137), Expect = 2e-10 Identities = 45/157 (28%), Positives = 90/157 (57%), Gaps = 4/157 (2%) Query: 371 GMNANAKEEASIAIGYNSKSLKKHTIAVGMHANSEGEASISIGYGAKTSNNYSISLGRYA 430 G+ + A G N+ + H+IA+G A + A++++G G+ + S+++G + Sbjct: 48 GLEYPVRPPVPGAGGLNASAKGIHSIAIGATAEAAKGAAVAVGAGSIATGVNSVAIGPLS 107 Query: 431 ESKGTDSLSIGSESVAEKNSAIAIGRKAKSKEDNSISIGQSSQSFKSNSITFGTSA--MT 488 ++ G +++ G+ S A+K+ +AIG +A S D +++G +S++ NS+ G S+ Sbjct: 108 KALGDSAVTYGAASTAQKD-GVAIGARA-STSDTGVAVGFNSKADAKNSVAIGHSSHVAA 165 Query: 489 NGESSIAVGTNSKSEGAHAIAIGKESRSEKLDALSIG 525 N SIA+G SK++ ++++IG ES + +L L+ G Sbjct: 166 NHGYSIAIGDRSKTDRENSVSIGHESLNRQLTHLAAG 202 Score = 53.4 bits (127), Expect = 4e-09 Identities = 40/130 (30%), Positives = 72/130 (55%), Gaps = 5/130 (3%) Query: 553 GLWSEAAAMNAIAIGGNAMSKASGSIAIGVSSKVEEDAINGIAIGKSSKSSKEHSIAIGT 612 GL + A +++IAIG A + ++A+G S +N +AIG SK+ + ++ G Sbjct: 62 GLNASAKGIHSIAIGATAEAAKGAAVAVGAGSIAT--GVNSVAIGPLSKALGDSAVTYGA 119 Query: 613 NTEAKDDGAIALGDNAKAVKNAISIGKESKSSDENSIAFGYNSEAKGKH--SISIGAYAV 670 + A+ DG +A+G A +++G SK+ +NS+A G++S H SI+IG + Sbjct: 120 ASTAQKDG-VAIGARASTSDTGVAVGFNSKADAKNSVAIGHSSHVAANHGYSIAIGDRSK 178 Query: 671 SENTNDISLG 680 ++ N +S+G Sbjct: 179 TDRENSVSIG 188 Score = 49.1 bits (116), Expect = 9e-08 Identities = 61/218 (27%), Positives = 114/218 (52%), Gaps = 14/218 (6%) Query: 638 GKESKSSDENSIAFGYNSEAKGKHSISIGAYAVSENTNDISLGLYAKAKGGKSIAIGQSS 697 G + + +SIA G +EA ++++GA +++ N +++G +KA G ++ G +S Sbjct: 62 GLNASAKGIHSIAIGATAEAAKGAAVAVGAGSIATGVNSVAIGPLSKALGDSAVTYGAAS 121 Query: 698 EALKSDSIAIGRHSKVTVDESVAIG--SESETKTPVA---SLNVTIE---NLKYGNFAAP 749 A K D +AIG + T D VA+G S+++ K VA S +V ++ G+ + Sbjct: 122 TAQK-DGVAIGARAS-TSDTGVAVGFNSKADAKNSVAIGHSSHVAANHGYSIAIGDRSKT 179 Query: 750 NVSHVVAIGSKNKERQLQYVGAGQVSKDSTDAINGSQLFATNNILGKFANKTKSIFGGNA 809 + + V+IG ++ RQL ++ AG +KD TDA+N +QL + NK + NA Sbjct: 180 DRENSVSIGHESLNRQLTHLAAG--TKD-TDAVNVAQLKKEIEKTQENTNKRSAELLANA 236 Query: 810 NLAENGELTFTNIGGTNKDTIHEAIKSLDDKITKSIAE 847 N A + + +G N T ++ ++L++ ++ A+ Sbjct: 237 N-AYADNKSSSVLGIANNYTDSKSAETLENARKEAFAQ 273 Score = 38.0 bits (87), Expect = 3e-04 Identities = 40/172 (23%), Positives = 86/172 (50%), Gaps = 9/172 (5%) Query: 356 IGEAAGAEGKYSITAGMNANAKEEASIAIGYNSKSLKKHTIAVGMHANSEGEASISIGYG 415 IG + A G ++T G + A+++ +AIG + S +AVG ++ ++ + S++IG+ Sbjct: 103 IGPLSKALGDSAVTYGAASTAQKDG-VAIGARA-STSDTGVAVGFNSKADAKNSVAIGHS 160 Query: 416 AKTSNN--YSISLGRYAESKGTDSLSIGSESVAEKNSAIAIGRKAK-----SKEDNSISI 468 + + N YSI++G +++ +S+SIG ES+ + + +A G K ++ I Sbjct: 161 SHVAANHGYSIAIGDRSKTDRENSVSIGHESLNRQLTHLAAGTKDTDAVNVAQLKKEIEK 220 Query: 469 GQSSQSFKSNSITFGTSAMTNGESSIAVGTNSKSEGAHAIAIGKESRSEKLD 520 Q + + +S + +A + +SS +G + + + + +R E Sbjct: 221 TQENTNKRSAELLANANAYADNKSSSVLGIANNYTDSKSAETLENARKEAFA 272
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Smon_1485	OMADHESIN	55	4e-10	Yersinia outer membrane adhesin signature.
>OMADHESIN#Yersinia outer membrane adhesin signature. Length = 455 Score = 55.3 bits (132), Expect = 4e-10 Identities = 62/183 (33%), Positives = 96/183 (52%), Gaps = 34/183 (18%) Query: 81 GANSKANGDNAFAIGNGATAEKNSAMSFGGKSSGTGATN--IGYQGESNGDSAVSIGYSS 138 G N+ A G ++ AIG A A K +A++ G S TG + IG ++ GDSAV+ G +S Sbjct: 62 GLNASAKGIHSIAIGATAEAAKGAAVAVGAGSIATGVNSVAIGPLSKALGDSAVTYGAAS 121 Query: 139 KSKENYATAIGGQSEASKTLSTAIGSAAKAKNQSSTAIGASSE--ASYDNAIAIGSSSKT 196 ++++ AIG ++ S T A+G +KA ++S AIG SS A++ +IAIG SKT Sbjct: 122 TAQKD-GVAIGARASTSDT-GVAVGFNSKADAKNSVAIGHSSHVAANHGYSIAIGDRSKT 179 Query: 197 DSKATQEKSVIIGDYTYSGFAGEAGDDGYQVSVGAKDKERQIKNVAAGKVSKESTDSVNG 256 D + + VS+G + RQ+ ++AAG + TD+VN Sbjct: 180 DRENS-------------------------VSIGHESLNRQLTHLAAG---TKDTDAVNV 211 Query: 257 SQL 259 +QL Sbjct: 212 AQL 214 Score = 38.0 bits (87), Expect = 1e-04 Identities = 66/351 (18%), Positives = 131/351 (37%), Gaps = 37/351 (10%) Query: 34 GAKATGESSIALGINANASGKHDVAIGTDAKTNTGENKGSNGGAVAIGANSKANGDNAFA 93 A A G SIA G+N +VAIG SKA GD+A Sbjct: 85 AAVAVGAGSIATGVN----------------------------SVAIGPLSKALGDSAVT 116 Query: 94 IGNGATAEKNSAMSFGGKSSGTGATNIGYQGESNGDSAVSIGYSSK--SKENYATAIGGQ 151 G +TA+K+ S+ +G+ +++ ++V+IG+SS + Y+ AIG + Sbjct: 117 YGAASTAQKDGVAIGARASTSDTGVAVGFNSKADAKNSVAIGHSSHVAANHGYSIAIGDR 176 Query: 152 SEASKTLSTAIGSAAKAKNQSSTAIGASSEASYDNAIAIGSSSKTDSKATQEKSVIIGDY 211 S+ + S +IG + + + A G + + A KT + + ++ + Sbjct: 177 SKTDRENSVSIGHESLNRQLTHLAAGTKDTDAVNVAQLKKEIEKTQENTNKRSAELLANA 236 Query: 212 TYSGFAGEAGDDGYQVSVGAKDKERQIKNVAAGKVSKESTDSVNGSQLFATNNALSNLMK 271 + G + ++N A + +S D +N ++ + + A + L Sbjct: 237 NAYADNKSSSVLGIANNYTDSKSAETLEN-ARKEAFAQSKDVLNMAKAHSNSVARTTLET 295 Query: 272 TTKTILGGNSKIIKTGDNIGN------ITMTDIGGTKKDNINDAIKAAITEVKAENKSAI 325 + ++T + N + ++ K + + T+V N + Sbjct: 296 AEEHANSVARTTLETAEEHANKKSAEALASANVYADSKSSHTLKTANSYTDVTVSNSTKK 355 Query: 326 DVKSTTGPNGNKIYTLDVKVDNKTIIKDKDGILKANVGTITTQINNGKIEF 376 ++ + +K LD ++D DK A + ++ GK+ F Sbjct: 356 AIRESNQYTDHKFRQLDNRLDKLDTRVDKGLASSAALNSLFQPYGVGKVNF 406 Score = 33.3 bits (75), Expect = 0.003 Identities = 45/181 (24%), Positives = 79/181 (43%), Gaps = 18/181 (9%) Query: 14 SIISYSNPSTPSPSNPTLGDGAKATGESSIALGINANASGKHDVAIGTDAKTNT-----G 68 SI + + +G G+ ATG +S+A+G + A G V G + G Sbjct: 72 SIAIGATAEAAKGAAVAVGAGSIATGVNSVAIGPLSKALGDSAVTYGAASTAQKDGVAIG 131 Query: 69 ENKGSNGGAVAIGANSKANGDNAFAIGNGATAEKNSAMSFGGKSSGTGATNIGYQGESNG 128 ++ VA+G NSKA+ N+ AIG+ + N S IG + +++ Sbjct: 132 ARASTSDTGVAVGFNSKADAKNSVAIGHSSHVAANHGYSIA----------IGDRSKTDR 181 Query: 129 DSAVSIGYSSKSKENYATAIGGQSEAS---KTLSTAIGSAAKAKNQSSTAIGASSEASYD 185 +++VSIG+ S +++ A G + + L I + N+ S + A++ A D Sbjct: 182 ENSVSIGHESLNRQLTHLAAGTKDTDAVNVAQLKKEIEKTQENTNKRSAELLANANAYAD 241 Query: 186 N 186 N Sbjct: 242 N 242
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Smon_1486	OMADHESIN	60	3e-11	Yersinia outer membrane adhesin signature.
>OMADHESIN#Yersinia outer membrane adhesin signature. Length = 455 Score = 59.9 bits (144), Expect = 3e-11 Identities = 46/119 (38%), Positives = 69/119 (57%), Gaps = 4/119 (3%) Query: 403 IGEAAGATGYGAIAVGIKTKASGLNSIAIGKSSNSSGDNSISMGIDSKASGLTSMAIGVD 462 IG A A A+AVG + A+G+NS+AIG S + GD++++ G S A +AIG Sbjct: 75 IGATAEAAKGAAVAVGAGSIATGVNSVAIGPLSKALGDSAVTYGAASTAQK-DGVAIGAR 133 Query: 463 AKASGITSIAIGSNANASGFGAISLGLSSSSSGMH--SLAIGQLAKASEEKSVALGSES 519 A S T +A+G N+ A ++++G SS + H S+AIG +K E SV++G ES Sbjct: 134 ASTSD-TGVAVGFNSKADAKNSVAIGHSSHVAANHGYSIAIGDRSKTDRENSVSIGHES 191 Score = 56.1 bits (134), Expect = 4e-10 Identities = 53/165 (32%), Positives = 91/165 (55%), Gaps = 13/165 (7%) Query: 418 GIKTKASGLNSIAIGKSSNSSGDNSISMGIDSKASGLTSMAIGVDAKASGITSIAIGSNA 477 G+ A G++SIAIG ++ ++ ++++G S A+G+ S+AIG +KA G +++ G+ + Sbjct: 62 GLNASAKGIHSIAIGATAEAAKGAAVAVGAGSIATGVNSVAIGPLSKALGDSAVTYGAAS 121 Query: 478 NASGFG-AISLGLSSSSSGMHSLAIGQLAKASEEKSVALGSESETTKAVATKDIEINGIK 536 A G AI S+S +G +A+G +KA + SVA+G S I Sbjct: 122 TAQKDGVAIGARASTSDTG---VAVGFNSKADAKNSVAIGHSSHVAANHGYS------IA 172 Query: 537 YGEFAGKTPFSVISIGYKDRERQLQYVAAGQISKESTDAINGSQL 581 G+ + + +SIG++ RQL ++AAG + TDA+N +QL Sbjct: 173 IGDRSKTDRENSVSIGHESLNRQLTHLAAG---TKDTDAVNVAQL 214 Score = 51.8 bits (123), Expect = 9e-09 Identities = 48/212 (22%), Positives = 91/212 (42%), Gaps = 5/212 (2%) Query: 98 GTDSKATALDAIAIGNKAHASTKYGIAIGTETKTDGIGAISLGYKSEANSDS-IAIGKEA 156 G ++ A + +IAIG A A+ +A+G + G+ ++++G S+A DS + G + Sbjct: 62 GLNASAKGIHSIAIGATAEAAKGAAVAVGAGSIATGVNSVAIGPLSKALGDSAVTYGAAS 121 Query: 157 VGYGKGIALGENSHSRRYSVAIGYMADSKGVYSYGNVVIGSESSTDENLNFSTAIGYKAK 216 G+A+G + + VA+G+ + + +V IG S N +S AIG ++K Sbjct: 122 TAQKDGVAIGARASTSDTGVAVGF---NSKADAKNSVAIGHSSHVAANHGYSIAIGDRSK 178 Query: 217 VEESYSTAIGSSSIAKKRDDRLGYDMFTNKVVKLEDKLEKADKSKYTKLKEEIEKLKAEK 276 + S +IG S+ ++ L V + ++ +K++ K E L Sbjct: 179 TDRENSVSIGHESLNRQL-THLAAGTKDTDAVNVAQLKKEIEKTQENTNKRSAELLANAN 237 Query: 277 GTLDKDAKDIIDKAYNSRTEDEKNKLTELNKK 308 D + ++ A N L K+ Sbjct: 238 AYADNKSSSVLGIANNYTDSKSAETLENARKE 269 Score = 48.0 bits (113), Expect = 2e-07 Identities = 41/113 (36%), Positives = 68/113 (60%), Gaps = 13/113 (11%) Query: 33 GSNSITTDTHNVQIGKDSKSEEDKKSIAIGSSSMSRGTNGVAIGNKAHAGAMPNLSNDVE 92 G+ SI T ++V IG SK+ D ++ G++S ++ +GVAIG +A + Sbjct: 90 GAGSIATGVNSVAIGPLSKALGDS-AVTYGAASTAQ-KDGVAIGARASTS---------D 138 Query: 93 NTVAIGTDSKATALDAIAIGNKAH--ASTKYGIAIGTETKTDGIGAISLGYKS 143 VA+G +SKA A +++AIG+ +H A+ Y IAIG +KTD ++S+G++S Sbjct: 139 TGVAVGFNSKADAKNSVAIGHSSHVAANHGYSIAIGDRSKTDRENSVSIGHES 191 Score = 35.3 bits (80), Expect = 0.001 Identities = 27/70 (38%), Positives = 38/70 (54%), Gaps = 1/70 (1%) Query: 336 EVSIGDAKNGITRQITGLAAGKEDTDAVNVAQLKSLYNKF-ESENKTYFHVNSGENKDTG 394 E S+ + RQ+T LAAG +DTDAVNVAQLK K E+ NK + + N Sbjct: 182 ENSVSIGHESLNRQLTHLAAGTKDTDAVNVAQLKKEIEKTQENTNKRSAELLANANAYAD 241 Query: 395 DKDTNLGKIG 404 +K +++ I Sbjct: 242 NKSSSVLGIA 251
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Smon_1487	PF03895	50	9e-10	Serum resistance protein DsrA.
>PF03895#Serum resistance protein DsrA. Length = 79 Score = 50.2 bits (120), Expect = 9e-10 Identities = 21/76 (27%), Positives = 40/76 (52%), Gaps = 4/76 (5%) Query: 1672 NGVANAVAMANLPQVSAIGDKRHNIAGSYGYYNGENAFALGL-SGVNETGTLVYRASGAL 1730 G+AN A++ L Q + +G + +++ + G Y + A A+G+ S + + T + Sbjct: 7 TGLANQSALSMLVQPNGVG--KTSVSAAVGGYRDKTALAIGVGSRITDRFTAKAGVAFNT 64 Query: 1731 NTKGHVSLGAGLGYQF 1746 G +S GA +GY+F Sbjct: 65 YN-GGMSYGASVGYEF 79
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Smon_1490	HTHFIS	94	2e-24	FIS bacterial regulatory protein HTH signature.
>HTHFIS#FIS bacterial regulatory protein HTH signature. Length = 484 Score = 93.7 bits (233), Expect = 2e-24 Identities = 32/118 (27%), Positives = 59/118 (50%) Query: 2 KILLVEDEIDLNNIIKKYLKTSGYIVDSVFDGEEALFNLNEASYDLVILDVMLPKLSGFE 61 IL+ +D+ + ++ + L +GY V + + DLV+ DV++P + F+ Sbjct: 5 TILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAFD 64 Query: 62 VLEKMRKKNNKAAVLMLTSKDQVEDKIKGLDLGADDYLSKPFDFEELAARIRAIIRRR 119 +L +++K VL++++++ IK + GA DYL KPFD EL I + Sbjct: 65 LLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAEP 122
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Smon_1491	PF06580	34	0.001	Sensor histidine kinase
>PF06580#Sensor histidine kinase Length = 349 Score = 33.7 bits (77), Expect = 0.001 Identities = 35/185 (18%), Positives = 67/185 (36%), Gaps = 44/185 (23%) Query: 269 IKRQSKKMSELISQIMMLSKIENTYNLELVDLNLSTL------VDNTLIDNSIIFKERNI 322 I K E+++ + L + Y+L + +L VD+ L SI F++R + Sbjct: 186 ILEDPTKAREMLTSLSELMR----YSLRYSNARQVSLADELTVVDSYLQLASIQFEDR-L 240 Query: 323 NLNKDIQKDIIISGNKIMLERM-LDNLIDNAIK------FTKDNVSVNLFKNDNKVVLEI 375 I I + + M + L++N IK + + K++ V LE+ Sbjct: 241 QFENQINPAI----MDVQVPPMLVQTLVENGIKHGIAQLPQGGKILLKGTKDNGTVTLEV 296 Query: 376 IDNGMGISEKNIDFIFNRFYQENVSRNKGKNQGSGLGLSLVKEIVKLHH---AEIKVESL 432 + G K + +G GL V+E +++ + A+IK+ Sbjct: 297 ENTGSLA-------------------LKNTKESTGTGLQNVRERLQMLYGTEAQIKLSEK 337 Query: 433 PKNYT 437 Sbjct: 338 QGKVN 342
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Smon_1495	CHANLCOLICIN	27	0.029	Channel forming colicin signature.
>CHANLCOLICIN#Channel forming colicin signature. Length = 522 Score = 26.6 bits (58), Expect = 0.029 Identities = 12/74 (16%), Positives = 23/74 (31%), Gaps = 8/74 (10%) Query: 41 NEFIIPSDDIVQTG--------VKGAAVGGYVGAKAGAVIGAAVGGPIGAEVGSFVGGAV 92 + + I TG ++ A V + G +G + V G + Sbjct: 445 YDVVSDILKIKDTGDWKPLFLTLEKKAADAGVSYVVALLFSLLAGTTLGIWGIAIVTGIL 504 Query: 93 GGYIGDKLGDKISD 106 YI + I++ Sbjct: 505 CSYIDKNKLNTINE 518