:: PredictBias ::

A) Input parameters
Genome	sequence.gb	Threshold dinucleotide bias	2
Threshold codon bias	4	Threshold %GC bias	3
E-value (RPSBlast)	0.05	Genome (non-pathogenic)

B) Compare a potential GI or PAI in related non-pathogenic sp. (phylogenetic tree)
Potential GI or PAI start end
Select Organism

C) Potential GIs and PAIs in NZ_CP007755 (download)

S.No

Start

End

Bias

Virulence

Insertion elements

Prediction

EN10_RS00070

EN10_RS08065

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
EN10_RS00070	1	21	-3.193419	large conductance mechanosensitive channel
EN10_RS00075	1	17	-0.527205	hypothetical protein
EN10_RS00090	1	18	-0.239756	**M48 family metallopeptidase
EN10_RS00095	0	16	-0.401691	hypothetical protein
EN10_RS00100	0	16	-0.322859	MarR family transcriptional regulator
EN10_RS08405	-1	19	-0.600364	DNA-binding protein
EN10_RS00110	-1	19	-0.119810	excinuclease ABC subunit UvrA
EN10_RS00115	0	25	-3.258093	class I SAM-dependent methyltransferase
EN10_RS00120	1	30	-4.436272	hypothetical protein
EN10_RS00125	2	31	-4.966802	NAD(P)H-dependent oxidoreductase
EN10_RS00130	4	40	-7.347294	hypothetical protein
EN10_RS00135	8	57	-14.282984	hypothetical protein
EN10_RS00140	9	55	-12.869971	hypothetical protein
EN10_RS00145	8	54	-12.465662	hypothetical protein
EN10_RS00150	7	53	-11.409517	nucleotide pyrophosphohydrolase
EN10_RS00155	7	55	-11.347496	hypothetical protein
EN10_RS00160	5	52	-10.924223	transporter
EN10_RS00170	4	47	-9.044703	DNA cytosine methyltransferase
EN10_RS00175	3	46	-8.891137	hypothetical protein
EN10_RS00180	3	45	-8.603258	hypothetical protein
EN10_RS00185	2	41	-8.046542	hypothetical protein
EN10_RS00190	2	40	-7.991424	hypothetical protein
EN10_RS08065	2	40	-5.895477	hypothetical protein

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
EN10_RS00070	MECHCHANNEL	93	6e-27	Bacterial mechano-sensitive ion channel signature.
>MECHCHANNEL#Bacterial mechano-sensitive ion channel signature. Length = 136 Score = 92.6 bits (230), Expect = 6e-27 Identities = 42/147 (28%), Positives = 70/147 (47%), Gaps = 17/147 (11%) Query: 1 MIDGFKKFIARGNMVDMAVGVVMGAAVTAVVNSVVDGIINPLVAAIFGKPDLSKTWNWTI 60 +I F++F RGN+VD+AVGV++GAA +V+S+V II P + + G D + Sbjct: 3 IIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPPLGLLIGGIDFKQFAVTLR 62 Query: 61 TNWAGENSV-ISFGSVLNALLNFLLIALAVYFCIVVPINNLRDMTEKAMAKVKGDDSDVA 119 +V + +G + + +FL++A A++ I + R E Sbjct: 63 DAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKLNRKKEE-------------P 109 Query: 120 AEEPDLSPEEQTVILLQDIRNALAAQQ 146 A P + EE +LL +IR+ L Q Sbjct: 110 AAAPAPTKEE---VLLTEIRDLLKEQN 133
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
EN10_RS00135	SALVRPPROT	28	0.004	Salmonella virulence-associated 28kDa protein signature.
>SALVRPPROT#Salmonella virulence-associated 28kDa protein signature. Length = 241 Score = 27.8 bits (61), Expect = 0.004 Identities = 14/39 (35%), Positives = 19/39 (48%) Query: 24 SRHVAQILHGMPAFTEELQRELAEGKVVLGGCDIDIFHP 62 S++ A LH F E L+ L+E V+ G C HP Sbjct: 166 SQYSASFLHKTRQFIECLESRLSENGVISGQCPESDVHP 204
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
EN10_RS00155	DPTHRIATOXIN	26	0.043	Diphtheria toxin signature.
>DPTHRIATOXIN#Diphtheria toxin signature. Length = 567 Score = 25.9 bits (56), Expect = 0.043 Identities = 12/38 (31%), Positives = 24/38 (63%), Gaps = 1/38 (2%) Query: 29 HSIIESQLKQSIPADSVVEQHAREYLEQYPVVYIVHAE 66 H I++++ +S P +V E+ A++YLE++ + H E Sbjct: 255 HGPIKNKMSES-PNKTVSEEKAKQYLEEFHQTALEHPE 291
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
EN10_RS00180	PF07132	30	0.031	Harpin protein (HrpN)
>PF07132#Harpin protein (HrpN) Length = 356 Score = 30.4 bits (68), Expect = 0.031 Identities = 21/85 (24%), Positives = 31/85 (36%), Gaps = 6/85 (7%) Query: 592 VAVATGGAAYVFAPAIAPILAGPSVAGLSGA------ALTSASLAAVGGGSLAAGGLGMS 645 + + GG +F + PS + G L+ + GS+ GGLG Sbjct: 13 ITIKAGGNGGLFPSQSSQNGGSPSQSAFGGQRSNIAEQLSDIMTTMMFMGSMMGGGLGGG 72 Query: 646 GGVALIAGGGALLGAVTGGGAATLG 670 G + GG G + GG LG Sbjct: 73 LGGLGSSLGGLGGGLLGGGLGGGLG 97
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
EN10_RS00190	GPOSANCHOR	39	7e-05	Gram-positive coccus surface protein anchor signature.
>GPOSANCHOR#Gram-positive coccus surface protein anchor signature. Length = 539 Score = 38.9 bits (90), Expect = 7e-05 Identities = 26/172 (15%), Positives = 52/172 (30%), Gaps = 26/172 (15%) Query: 255 DVLEQAFEKPANLEEYLG-AKPSHNDVRALQTAIKATIRSNTDDVSEI--IYDVLKNDDD 311 + A LE+ L A A ++A + +++ + N Sbjct: 183 AEKAALEARQAELEKALEGAMNFSTADSAKIKTLEAEKAALAARKADLEKALEGAMNFST 242 Query: 312 VMAEFRKQATAQLDD------EFEKHKQVLCKQLDELRAQRDEESEKAEQATAQLAELNQ 365 + K A+ E EK + A+ + A+ A+L Sbjct: 243 ADSAKIKTLEAEKAALEARQAELEKALEGAMNFSTADSAKIKTLEAEKAALEAEKADLEH 302 Query: 366 QLQRKRGEI----------RDAQAELE-----LQKEEQ--HKALQDLENNVA 400 Q Q R+A+ +LE L+++ + + Q L ++ Sbjct: 303 QSQVLNANRQSLRRDLDASREAKKQLEAEHQKLEEQNKISEASRQSLRRDLD 354 Score = 38.5 bits (89), Expect = 1e-04 Identities = 26/107 (24%), Positives = 47/107 (43%), Gaps = 5/107 (4%) Query: 306 LKNDDDVMAEFRKQATAQLDDEFEKHKQVLCKQLDELRAQRDEESEKAEQATAQLAELNQ 365 L+ D D E +KQ A+ + E+ ++ LR D E +Q L E N Sbjct: 349 LRRDLDASREAKKQLEAEHQ-KLEEQNKISEASRQSLRRDLDASREAKKQVEKALEEANS 407 Query: 366 QLQRKRGEIRDAQAELELQKEEQHKALQDLENNV-ALKLGLQQIAQQ 411 +L ++ + +L ++E+ + LE ALK +++A+Q Sbjct: 408 KLAALEKLNKELEESKKLTEKEKAELQAKLEAEAKALK---EKLAKQ 451

EN10_RS00660

EN10_RS00705

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
EN10_RS00660	-1	24	3.438267	branched-chain amino acid ABC transporter
EN10_RS00665	1	26	2.782188	branched-chain amino acid ABC transporter
EN10_RS00670	2	26	3.067450	ABC transporter ATP-binding protein
EN10_RS00675	2	27	3.119814	ABC transporter ATP-binding protein
EN10_RS00680	2	23	3.743955	ABC transporter substrate-binding protein
EN10_RS00685	1	19	3.790962	LacI family DNA-binding transcriptional
EN10_RS00690	1	18	3.899846	sucrose phosphorylase
EN10_RS00695	1	15	4.640121	MFS transporter
EN10_RS00700	2	16	4.230299	ABC transporter substrate-binding protein
EN10_RS00705	2	15	3.890173	biotin carboxylase

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
EN10_RS00675	PF05272	30	0.008	Virulence-associated E family protein
>PF05272#Virulence-associated E family protein Length = 892 Score = 30.4 bits (68), Expect = 0.008 Identities = 10/17 (58%), Positives = 12/17 (70%) Query: 63 GPNGAGKSTLLKSLFGL 79 G G GKSTL+ +L GL Sbjct: 603 GTGGIGKSTLINTLVGL 619
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
EN10_RS00695	TCRTETB	39	4e-05	Tetracycline resistance protein TetB signature.
>TCRTETB#Tetracycline resistance protein TetB signature. Length = 458 Score = 39.1 bits (91), Expect = 4e-05 Identities = 64/376 (17%), Positives = 121/376 (32%), Gaps = 60/376 (15%) Query: 163 VNSIGAIVALLANIIFGTLSDMTRSRFGKRTPWIVGGGIITGLAIGAIAFTRNEA-LIIF 221 VN+ + + ++G LSD + G + + G II + L+I Sbjct: 54 VNTAFMLTFSIGTAVYGKLSD----QLGIKRLLLFGI-IINCFGSVIGFVGHSFFSLLIM 108 Query: 222 LWCVAQMGYNMMLAPYVAVMSDRVPDKVRGTISGFYGAGIAVGQTLGSVVGAAFLRQGQS 281 + G A + V++ +P + RG G G+ +A+G+ +G +G Sbjct: 109 ARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGMIAHYIHW 168 Query: 282 GLFSAWMMGLGIFSLTGIFVVAMWPREKSSRFEEQSEHVTVKSVLLNF------------ 329 M I +T F++ + +E + + + + SV + F Sbjct: 169 SYLLLIPM---ITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTTSYSISF 225 Query: 330 -------------------RPPRHAPDFYYALVGRTLMMGGYWMITTYQLYIAQDYIYAG 370 P ++ GG T Y+ Sbjct: 226 LIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVSMVPYMM-- 283 Query: 371 DPNAKDKAATIIATMGVITLVVSLIAAVTAGPIT----DRLGRRKLPVALASCLFAVG-- 424 KD A +G + + ++ + G I DR G + + + +V Sbjct: 284 ----KDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYV-LNIGVTFLSVSFL 338 Query: 425 -AAMPLFFRSPMGMYLFAAVAGLGYGVYNAIDQALNVAVLP--NPKEAGKDLGILNLANT 481 A+ L S + V G + ++ V +EAG + +LN + Sbjct: 339 TASFLLETTSWFMTIIIVFV----LGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTSF 394 Query: 482 LSTVIGTAMTSLVVSI 497 LS G A+ ++SI Sbjct: 395 LSEGTGIAIVGGLLSI 410

EN10_RS08480

EN10_RS01210

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
EN10_RS08480	2	20	1.722124	MarR family transcriptional regulator
EN10_RS00835	2	23	1.857886	ABC transporter ATP-binding protein/permease
EN10_RS00840	1	24	2.114786	ABC transporter ATP-binding protein/permease
EN10_RS00845	1	19	1.630486	polysaccharide deacetylase family protein
EN10_RS00855	1	18	1.125664	phosphatase PAP2 family protein
EN10_RS00860	2	20	1.393059	sugar O-acetyltransferase
EN10_RS00865	2	18	1.395990	hypothetical protein
EN10_RS00870	0	17	1.328786	ABC transporter substrate-binding protein
EN10_RS00875	-1	17	2.353078	TetR/AcrR family transcriptional regulator
EN10_RS00880	-1	17	2.238003	YhgE/Pip domain-containing protein
EN10_RS00885	-2	15	2.560808	YhgE/Pip domain-containing protein
EN10_RS00890	0	13	2.958541	MptD family putative ECF transporter S
EN10_RS00895	0	14	3.355012	energy-coupling factor transporter transmembrane
EN10_RS00900	1	16	2.983596	ATP-binding cassette domain-containing protein
EN10_RS00905	1	18	2.348873	ZinT/AdcA family metal-binding protein
EN10_RS00910	1	21	3.248438	patatin family protein
EN10_RS00915	-1	17	3.132599	hypothetical protein
EN10_RS00920	-1	17	3.249912	hypothetical protein
EN10_RS00930	0	15	3.467924	*C69 family dipeptidase
EN10_RS00935	1	13	3.700480	TetR/AcrR family transcriptional regulator
EN10_RS00940	1	16	3.769139	signal recognition particle-docking protein
EN10_RS00945	-2	16	3.506825	ammonium transporter
EN10_RS00950	-1	18	3.726568	P-II family nitrogen regulator
EN10_RS00955	0	18	3.482428	nucleotidyltransferase domain-containing
EN10_RS00960	1	20	2.356268	helix-turn-helix domain-containing protein
EN10_RS00965	1	20	2.681156	replicative DNA helicase
EN10_RS00970	1	18	2.756820	Mur ligase family protein
EN10_RS00975	2	19	2.585243	glutamine amidotransferase
EN10_RS00980	3	20	2.658751	phasin family protein
EN10_RS00985	4	20	2.624798	AarF/UbiB family protein
EN10_RS00990	0	17	3.511791	glycerophosphodiester phosphodiesterase
EN10_RS00995	-1	16	3.798330	uracil phosphoribosyltransferase
EN10_RS01000	1	16	4.298140	hypothetical protein
EN10_RS01005	2	16	4.044983	NUDIX hydrolase
EN10_RS01010	3	17	4.197335	RNA degradosome polyphosphate kinase
EN10_RS01015	10	26	5.623270	septum formation initiator
EN10_RS01020	10	24	5.826380	Flp pilus assembly complex ATPase component
EN10_RS01025	6	16	5.131972	hypothetical protein
EN10_RS01030	3	14	4.444458	type II secretion system F family protein
EN10_RS01035	1	13	4.536371	DUF4244 domain-containing protein
EN10_RS01040	0	14	4.576725	pilus assembly protein
EN10_RS01045	-2	15	4.273130	hypothetical protein
EN10_RS01050	-2	15	3.851252	DNA polymerase III subunit gamma/tau
EN10_RS01055	0	19	2.984841	recombination mediator RecR
EN10_RS01060	0	21	2.649421	aspartate kinase
EN10_RS01065	0	22	2.389758	ACT domain-containing protein
EN10_RS01070	0	20	2.769553	aspartate-semialdehyde dehydrogenase
EN10_RS01075	1	20	3.278578	DUF5701 family protein
EN10_RS01080	2	21	3.481329	2-isopropylmalate synthase
EN10_RS01085	2	20	4.205551	penicillin-binding protein
EN10_RS01090	2	17	4.580783	inositol-3-phosphate synthase
EN10_RS01095	2	18	4.500699	phosphatase PAP2 family protein
EN10_RS01100	3	20	3.889910	type I DNA topoisomerase
EN10_RS01105	2	16	1.972518	dTMP kinase
EN10_RS01110	2	16	1.980170	DNA polymerase III subunit delta'
EN10_RS01115	2	16	1.219269	YbhB/YbcL family Raf kinase inhibitor-like
EN10_RS01120	2	13	0.066937	C69 family dipeptidase
EN10_RS01125	1	13	-0.989885	C69 family dipeptidase
EN10_RS01130	2	12	-1.181383	ATP-binding protein
EN10_RS01135	1	12	0.106859	formate--tetrahydrofolate ligase
EN10_RS01140	0	13	-0.157922	phenylpyruvate tautomerase MIF-related protein
EN10_RS01145	0	13	1.138066	DUF559 domain-containing protein
EN10_RS01150	0	17	2.590366	GtrA family protein
EN10_RS01155	1	18	2.956832	hypothetical protein
EN10_RS01160	1	19	3.269822	histidine phosphatase family protein
EN10_RS01165	2	20	3.435287	MFS transporter
EN10_RS01170	2	19	3.073252	glutamate--tRNA ligase
EN10_RS01175	5	21	2.046250	**metallophosphoesterase
EN10_RS01190	4	19	2.466656	IclR family transcriptional regulator
EN10_RS01195	4	21	3.071389	3-isopropylmalate dehydratase large subunit
EN10_RS01200	4	21	2.520228	3-isopropylmalate dehydratase small subunit
EN10_RS01210	2	19	3.053100	Lrp/AsnC ligand binding domain-containing

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
EN10_RS00875	HTHTETR	57	2e-12	TetR bacterial regulatory protein HTH signature.
>HTHTETR#TetR bacterial regulatory protein HTH signature. Length = 215 Score = 57.3 bits (138), Expect = 2e-12 Identities = 25/180 (13%), Positives = 57/180 (31%), Gaps = 18/180 (10%) Query: 38 RRERTDGKITRAVLELILAKGIGAVSIEAVAKRSGVAKTTIYRRYANTDDLL-------- 89 + T I L L +G+ + S+ +AK +GV + IY + + DL Sbjct: 8 EAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSEIWELSE 67 Query: 90 RHLSVAVGEPLDFSGFDTTFDGLRGVLKCIVDCFDEELGLKAIGVVLSSSNDY------L 143 ++ E LR +L +++ E + + ++ ++ + Sbjct: 68 SNIGELELEYQA-KFPGDPLSVLREILIHVLESTVTEERRRLLMEIIFHKCEFVGEMAVV 126 Query: 144 ANLAERVVTPAQQRFADFIGRGVSSGAFRGDVSVPFLFQTV---IGSMMAAKALSDSSHD 200 + + R + + + D+ + I +M + S D Sbjct: 127 QQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMTRRAAIIMRGYISGLMENWLFAPQSFD 186
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
EN10_RS00880	FLAGELLIN	33	0.006	Flagellin signature.
>FLAGELLIN#Flagellin signature. Length = 507 Score = 32.7 bits (74), Expect = 0.006 Identities = 32/300 (10%), Positives = 67/300 (22%), Gaps = 30/300 (10%) Query: 92 TFTKSSAEAIDGTKSGKYYAAVVIPKNFSKNMMTFFTDDAYHATLDYYNNEKLNALAPKV 151 + + + + + + T D NN ++ Sbjct: 199 AVGANKYRVDVNSGAVVTDTTA----PTVPDKVYVNAANGQLTTDDAENNTAVDLFKTTK 254 Query: 152 TGQGADTIAATINQTFSKTLTDTALNLASSMLDMLDKPEYQQRLKSFNGNIADLAGMLND 211 + G A DT + G Sbjct: 255 STAGTAEAKAIAGAIKGGKEGDTFDYKGVTFT---------------IDTKTGNDGNGKV 299 Query: 212 SAAVLRTYSSLTDSAKTLLASSNKLIADARASAKTGVDDLSAAKTGVSDLSGALGTSVNA 271 S + +LT + T A++ + + + S Sbjct: 300 STTINGEKVTLTVADITAGAANVDAATLQSSKNVYTSVVNGQFTFDDKTKNESAKLSDLE 359 Query: 272 LGTAIDTSKTSLGSLNDRVDELFSAVDKQAGDTSAAINNLAGNVRTEATAYATTRGRLQE 331 A+ + + AG T +G A + Sbjct: 360 ANNAVKGESKITVNGAEYTANAAGDKVTLAGKTMFIDKTASGVSTLINEDAAAAKKSTAN 419 Query: 332 LLDKSDNLGPVAKSAVQAFINRIGAVADDLNQTADRLTTAANDVDSKVGSSKETRQEIKD 391 L D+ ++++ AV L +R +A ++ + V + R I+D Sbjct: 420 PLASIDSA-----------LSKVDAVRSSLGAIQNRFDSAITNLGNTVTNLNSARSRIED 468
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
EN10_RS00885	ABC2TRNSPORT	34	0.003	ABC-2 type transport system membrane protein signat...
>ABC2TRNSPORT#ABC-2 type transport system membrane protein signature. Length = 262 Score = 33.8 bits (77), Expect = 0.003 Identities = 23/98 (23%), Positives = 45/98 (45%), Gaps = 3/98 (3%) Query: 604 LTSLAYLAIAYALSATFMHVGKALVVALVMLQIPGASGLYPIEMMPDFYRALYPFFPFTY 663 SL + A A S + + LV+ +L + GA ++P++ +P ++ F P ++ Sbjct: 161 FASLGMVVTALAPSYDYFIFYQTLVIT-PILFLSGA--VFPVDQLPIVFQTAARFLPLSH 217 Query: 664 SINAFRETIAGFYDGDWLTKMLMLALFTAIAFFVGLVV 701 SI+ R + G D + L ++ I FF+ + Sbjct: 218 SIDLIRPIMLGHPVVDVCQHVGALCIYIVIPFFLSTAL 255
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
EN10_RS00905	adhesinb	102	3e-26	Adhesin B signature.
>adhesinb#Adhesin B signature. Length = 310 Score = 102 bits (255), Expect = 3e-26 Identities = 51/307 (16%), Positives = 111/307 (36%), Gaps = 49/307 (15%) Query: 29 LAGLAVCGLAASTLSGCGNNAGENRTSAAAARQTTDSCDTTINVVASVNQWGSLAQQLGG 88 L + LA L+ C + +T++ + +NVVA+ + + + + G Sbjct: 4 CRFLVLLLLAFVGLAACSSQKS-----------STETGSSKLNVVATNSIIADITKNIAG 52 Query: 89 SCVNVTSIINSTAADPHDHEATPADLTKLARADVVVLNG----AGYDGWAQKAQLDEGRQ 144 +N+ SI+ DPH++E P D+ K ++AD++ NG G + W K Sbjct: 53 DKINLHSIVPVGQ-DPHEYEPLPEDVKKTSQADLIFYNGINLETGGNAWFTKL---VENA 108 Query: 145 RIVKASSLMGIADSQDDHDHEEGEGHHHHHGTVNPHLWFSPAAVLKMSEAITSAYVTKSG 204 + + +++ D E G +PH W + + ++ I K Sbjct: 109 KKKENKDYYAVSEGVDVIYLEGQS----EKGKEDPHAWLNLENGIIYAQNIAKRLSEKDP 164 Query: 205 EASETAATARRHSNTWNAEYAEYTALVN------RARAKNL---QRRYVATESIIGHLLD 255 + T+ Y ++ + + N+ ++ V +E + Sbjct: 165 A----------NKETYEKNLKAYVEKLSALDKEAKEKFNNIPGEKKMIVTSEGCFKYFSK 214 Query: 256 YIGATDKTPDSYTNAMNNDAEPSASDLKNALDTVGSSNVDMLIVNPQEMGGFAKKLDAAA 315 P +Y +N + E + +K ++ + + V L V E + + + Sbjct: 215 AYN----VPSAYIWEINTEEEGTPDQIKTLVEKLRKTKVPSLFV---ESSVDDRPMKTVS 267 Query: 316 RESGKTI 322 +++ I Sbjct: 268 KDTNIPI 274
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
EN10_RS00915	HTHTETR	27	0.046	TetR bacterial regulatory protein HTH signature.
>HTHTETR#TetR bacterial regulatory protein HTH signature. Length = 215 Score = 27.3 bits (60), Expect = 0.046 Identities = 30/227 (13%), Positives = 63/227 (27%), Gaps = 39/227 (17%) Query: 19 TPTRQSIERALVEWMTQTGRPASDLSVSALCKKAFVARSTFYANYRHMGQVYESVENRLL 78 TRQ I + +Q G S S+ + K A V R Y +++ ++ + Sbjct: 10 QETRQHILDVALRLFSQQG--VSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSEIWELSE 67 Query: 79 RQLFECAVPMRVRADGTDETMRSFNAFVKVLHAHENEFRLVLVRQPSSRFVALWKQSLEY 138 + E + + + G + ++L + + + + E+ Sbjct: 68 SNIGELELEYQAKFPGDPLS-----VLREILIHV---LESTVTEERRRLLMEIIFHKCEF 119 Query: 139 H-VWHRLFETERGGAAAVDAVAEWGDGAAPGTSNGGSATSQEGLPAGTDADATNRNELPV 197 + + +R E + LPA Sbjct: 120 VGEMAVVQQAQRNLCLESYDRIEQTLKH---------CIEAKMLPADLMTRRA------- 163 Query: 198 RQGPATVNRALVFDMAATAFIAGVTFWLRTPQAVSLPEIRASVVKLV 244 A + R + + WL PQ+ L + V ++ Sbjct: 164 ----AIIMRGYISGLMEN--------WLFAPQSFDLKKEARDYVAIL 198
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
EN10_RS00935	HTHTETR	71	4e-17	TetR bacterial regulatory protein HTH signature.
>HTHTETR#TetR bacterial regulatory protein HTH signature. Length = 215 Score = 71.2 bits (174), Expect = 4e-17 Identities = 20/105 (19%), Positives = 46/105 (43%) Query: 1 MANASRMSSYQRREQLLRIGRSLFAAKGFEAVSVEEIAATAKVSKPIVYEHFGGKEGLYA 60 MA ++ + + R+ +L + LF+ +G + S+ EIA A V++ +Y HF K L++ Sbjct: 1 MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFS 60 Query: 61 VVVDREMRALNDTLVAALSAQDVHPRQIVERTALALLTYIEENAE 105 + + + + + + P ++ + +L Sbjct: 61 EIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVTEER 105
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
EN10_RS00940	IGASERPTASE	52	4e-09	IgA-specific serine endopeptidase (S6) signature.
>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature. Length = 1541 Score = 52.0 bits (124), Expect = 4e-09 Identities = 34/215 (15%), Positives = 68/215 (31%), Gaps = 20/215 (9%) Query: 29 SRRNAINKSTEHAKAAADARLAAERAQAEQEEREAHEQAKEPAESGARQSSGGKPAEPTN 88 S++ + A E A+ + +A+ Q E A+SG+ ++ + E Sbjct: 1044 SKQESKTVEKNEQDATETTAQNREVAKEAKSNVKANTQTNEVAQSGS-ETKETQTTETKE 1102 Query: 89 EEKIEAEEPAISRAELEAQERERLSQTPPKLAAAEEVPGEESAQAESVVEDLAVVDEVPT 148 +E EE A E + + SQ PK +E V + E PT Sbjct: 1103 TATVEKEEKAKVETEKTQEVPKVTSQVSPKQEQSETVQPQAEPARE----------NDPT 1152 Query: 149 PPAEQIEKPAGKKSRLQRLRERLSKSSNPFGKALFNILAKDQLSEA--DWEDVEDTLLLA 206 ++ + + ++ + S + + + + + A Sbjct: 1153 VNIKEPQSQTNTTADTEQPAKETSSNVEQ-------PVTESTTVNTGNSVVENPENTTPA 1205 Query: 207 DVGAEASEELVDKLRTDARVTGESDPRQLRDALRS 241 + E +K + R + S P + A S Sbjct: 1206 TTQPTVNSESSNKPKNRHRRSVRSVPHNVEPATTS 1240 Score = 30.4 bits (68), Expect = 0.021 Identities = 35/238 (14%), Positives = 65/238 (27%), Gaps = 13/238 (5%) Query: 35 NKSTEHAKAAADARLAAERAQAEQEEREAHEQAKEPAESGARQSSGGKPAEPTNEEKIEA 94 N E D + + +E A PA P+ + A Sbjct: 982 NPEVEKRNQTVDTTNITTPNNIQADVPSVPSNNEEIARVDEAPVPPPAPATPSETTETVA 1041 Query: 95 EEPAISRAELEAQERERLSQTPPKLAAAEEVPGEESA--QAESVVEDLAVVDEVPTPPAE 152 E +E E++ T A+E A Q V + + E T + Sbjct: 1042 ENSKQESKTVEKNEQDATETTAQNREVAKEAKSNVKANTQTNEVAQSGSETKETQTTETK 1101 Query: 153 Q---IEKPAGKKSRLQRLRERLSKSSNPFGKALFNILAKDQLSEADWEDVEDTLLLADVG 209 + +EK K ++ +E +S K ++ +A+ D + Sbjct: 1102 ETATVEKEEKAKVETEKTQEVPKVTSQVSPK---QEQSETVQPQAEPARENDPTVNIK-- 1156 Query: 210 AEASEELVDKLRTDARVTGESDPRQLRDALRSELLALVGPDMDRTLEAEKPDAKKPSV 267 E + D + + + G + E P +P+V Sbjct: 1157 -EPQSQ--TNTTADTEQPAKETSSNVEQPVTESTTVNTGNSVVENPENTTPATTQPTV 1211
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
EN10_RS00985	IGASERPTASE	35	0.002	IgA-specific serine endopeptidase (S6) signature.
>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature. Length = 1541 Score = 34.7 bits (79), Expect = 0.002 Identities = 29/149 (19%), Positives = 52/149 (34%), Gaps = 6/149 (4%) Query: 2 SDKNSDKSKRETEAHELRRDVRR-EEAGQRRQQRKDACEARHLEHEEKRETSELID--LS 58 +D S S E A V A A ++ ++ + + Sbjct: 1005 ADVPSVPSNNEEIARVDEAPVPPPAPATPSETTETVAENSKQESKTVEKNEQDATETTAQ 1064 Query: 59 NRLENQEAHSDGPAFDNPTDDALRATVETAES-EKILHLGDEVDEQGATIEHNLQREEAE 117 NR +EA S+ A + T++ ++ ET E+ V+++ + +E Sbjct: 1065 NREVAKEAKSNVKA-NTQTNEVAQSGSETKETQTTETKETATVEKEEKAKVETEKTQEVP 1123 Query: 118 QSGGEASETAAQPETPAPQSAQSERPADA 146 + + S Q ET PQ A+ R D Sbjct: 1124 KVTSQVSPKQEQSETVQPQ-AEPARENDP 1151
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
EN10_RS01040	PilS_PF08805	28	0.013	PilS N terminal
>PilS_PF08805#PilS N terminal Length = 185 Score = 27.6 bits (61), Expect = 0.013 Identities = 18/65 (27%), Positives = 26/65 (40%), Gaps = 2/65 (3%) Query: 10 LRRLQCDDEGAATAEFAVVLPVIVML--AALMLYLGRASVVSVGCQDAAANAARALMVQG 67 RR + D+GA E +V+ VIV+L +A LY S + + A M Sbjct: 18 ARRKKEQDKGATLMEVLLVVGVIVVLAASAYKLYSMVQSNIQSSNEQNNVLTVIANMKSL 77 Query: 68 SGSGS 72 G Sbjct: 78 KFQGR 82
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
EN10_RS01050	IGASERPTASE	32	0.010	IgA-specific serine endopeptidase (S6) signature.
>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature. Length = 1541 Score = 32.3 bits (73), Expect = 0.010 Identities = 38/221 (17%), Positives = 68/221 (30%), Gaps = 27/221 (12%) Query: 567 APPLSKLPPEEAQRIKAKLLQQSLQGIVAHPRESSDSAQQATTSDSSNDAEAHQAAGTTP 626 PP P E + + Q+S + + Q AT + + N A +A Sbjct: 1026 PPPAPATPSETTETVAENSKQES--------KTVEKNEQDATETTAQNREVAKEAKSNVK 1077 Query: 627 TPTESFQQANGNGPQTLPDDDPWAQPVKPVGLRPQALDPEEQAHEEPNRQQPAPEHQHKH 686 T Q N + K ++ EE+A E + Q P+ + Sbjct: 1078 ANT----QTNEVAQSGSETKETQTTETK----ETATVEKEEKAKVETEKTQEVPKVTSQV 1129 Query: 687 VAVPDVSDGIDPWSMPVDEHPHIDFGKVQSIEQQHERLTPAPVHGDDPWSAMPNGPQGFG 746 + S+ + P + P E+ K + T P N Q Sbjct: 1130 SPKQEQSETVQPQAEPARENDPTVNIKEPQSQTNTTADTEQPAK-----ETSSNVEQ--- 1181 Query: 747 TASPVTGGQAMSVEDAYGMSPQGMHAADAGSTGVAGGESQP 787 PVT ++ ++ +P+ A T + ++P Sbjct: 1182 ---PVTESTTVNTGNSVVENPENTTPATTQPTVNSESSNKP 1219
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
EN10_RS01060	CARBMTKINASE	28	0.027	Bacterial carbamate kinase signature.
>CARBMTKINASE#Bacterial carbamate kinase signature. Length = 314 Score = 28.3 bits (63), Expect = 0.027 Identities = 22/124 (17%), Positives = 47/124 (37%), Gaps = 22/124 (17%) Query: 113 IRAVNPDRVRRALNKGSVAIVAG-------FQGQSERGDATTLGRGGSDTSAVALAVALG 165 V + +++ + +G + I +G + +G + + D + LA + Sbjct: 171 KGHVEAETIKKLVERGVIVIASGGGGVPVILEDGEIKGVEAVIDK---DLAGEKLAEEVN 227 Query: 166 ADVCEIYTDVDGVFTADPRIVPTARRIPAIGYDEILEM--------SSCGSKVLALRCVE 217 AD+ I TDV+G + + + +E+ + S G KVLA + Sbjct: 228 ADIFMILTDVNGAALYYGT--EKEQWLREVKVEELRKYYEEGHFKAGSMGPKVLA--AIR 283 Query: 218 YAQR 221 + + Sbjct: 284 FIEW 287
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
EN10_RS01065	OMPADOMAIN	28	0.030	OMPA domain signature.
>OMPADOMAIN#OMPA domain signature. Length = 346 Score = 27.6 bits (61), Expect = 0.030 Identities = 36/171 (21%), Positives = 62/171 (36%), Gaps = 9/171 (5%) Query: 23 ISGVAHDNSESLVTVRGVTDE--PGMAGRVFTR-LAELGVNVDMIVQAGASTGTADISFT 79 + G HD S V GV P +A R+ + +G + + + +S+ Sbjct: 131 VYGKNHDTGVSPVFAGGVEYAITPEIATRLEYQWTNNIGDAHTIGTRPDNGMLSLGVSYR 190 Query: 80 IPGSDAARVETALTAAQDTLGYKSFDVNPNV----GKVAVVGVGMKTHSGLAARFFQALS 135 +AA V A + K F + +V K + G L ++ Sbjct: 191 FGQGEAAPVVAPAPAPAPEVQTKHFTLKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDP 250 Query: 136 EEGINVLMISTSEIRIAAL-VPLSQLN-DAVRALHSAYGLDADKVEAVVYG 184 ++G V++ T I A LS+ +V + G+ ADK+ A G Sbjct: 251 KDGSVVVLGYTDRIGSDAYNQGLSERRAQSVVDYLISKGIPADKISARGMG 301
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
EN10_RS01085	cloacin	48	9e-08	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 48.2 bits (114), Expect = 9e-08 Identities = 28/76 (36%), Positives = 35/76 (46%), Gaps = 5/76 (6%) Query: 730 GSNSGDSGNSGNQNGNTSG---NNGGNNGSNNGSNNGSNNSGSNSG--DSGNSGNQNGNT 784 G N+G SGN NG +G G ++GS S N GS SG G SG+ NG Sbjct: 8 GHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGG 67 Query: 785 GGNNGSQNNTGNGEQT 800 GN+G + TG Sbjct: 68 NGNSGGGSGTGGNLSA 83 Score = 44.3 bits (104), Expect = 2e-06 Identities = 24/84 (28%), Positives = 35/84 (41%), Gaps = 3/84 (3%) Query: 708 GNDNTNGNGGG-ETGGNPSDNNGGSNSGDSGNSGNQNGNTSGNNGGNNGSNNGSNNGSNN 766 G D N G T GN + G G + G+ + + GG GS +G + G + Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGG--GSGSGIHWGGGS 60 Query: 767 SGSNSGDSGNSGNQNGNTGGNNGS 790 N G +GNSG +G G + Sbjct: 61 GHGNGGGNGNSGGGSGTGGNLSAV 84
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
EN10_RS01165	TCRTETA	30	0.020	Tetracycline resistance protein signature.
>TCRTETA#Tetracycline resistance protein signature. Length = 399 Score = 30.2 bits (68), Expect = 0.020 Identities = 17/59 (28%), Positives = 28/59 (47%), Gaps = 4/59 (6%) Query: 63 GALTAVQAIAGAVVGPLLGTLVDRFGQRQVSIPTIVIWMLAAVSFVTCVRAQVTPWILF 121 G L A+ A+ P+LG L DRFG+R P +++ + A + W+L+ Sbjct: 46 GILLALYALMQFACAPVLGALSDRFGRR----PVLLVSLAGAAVDYAIMATAPFLWVLY 100

EN10_RS02815

EN10_RS02865

Genomic Island

LocusTag	DNBias	CDNBias	%GCBias	Product
EN10_RS02815	-1	21	-4.173251	DUF559 domain-containing protein
EN10_RS02820	-1	18	-3.475648	GNAT family N-acetyltransferase
EN10_RS02825	1	18	-3.827399	hypothetical protein
EN10_RS08225	-1	23	-6.821633	hypothetical protein
EN10_RS08505	-1	29	-7.629470	hypothetical protein
EN10_RS02840	0	30	-8.242733	hypothetical protein
EN10_RS02845	1	30	-7.725040	(deoxy)nucleoside triphosphate
EN10_RS02850	1	29	-7.175003	DEAD/DEAH box helicase
EN10_RS02855	0	28	-7.110559	Fic family protein
EN10_RS08230	0	23	-5.329280	DUF262 domain-containing HNH endonuclease family
EN10_RS02865	0	15	-3.359690	DUF4065 domain-containing protein

EN10_RS02970

EN10_RS02995

Genomic Island

LocusTag	DNBias	CDNBias	%GCBias	Product
EN10_RS02970	-1	23	-3.683645	DNA methylase
EN10_RS02975	0	23	-5.439685	hypothetical protein
EN10_RS02980	-1	25	-5.038343	GTP-binding DUF697 domain-containing protein
EN10_RS02985	0	27	-6.529103	hypothetical protein
EN10_RS02990	-1	27	-5.698511	pyridoxamine 5'-phosphate oxidase family
EN10_RS02995	-1	21	-5.060204	XRE family transcriptional regulator

EN10_RS03160

EN10_RS03295

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
EN10_RS03160	2	13	-0.375376	proteasome accessory factor PafA2
EN10_RS03165	2	12	-0.697020	inositol monophosphatase family protein
EN10_RS03170	3	15	-0.827524	ubiquitin-like protein Pup
EN10_RS03175	3	15	-0.816359	proteasome accessory factor PafA2 family
EN10_RS03180	2	15	-0.985312	HU family DNA-binding protein
EN10_RS03185	2	15	-0.927791	flippase-like domain-containing protein
EN10_RS03190	2	16	-1.238733	adenylosuccinate lyase
EN10_RS03195	3	20	-1.313697	alpha/beta hydrolase
EN10_RS03200	2	21	-1.144949	hypothetical protein
EN10_RS03205	1	18	-0.906107	DUF6466 family protein
EN10_RS03210	2	18	-0.804529	VWA domain-containing protein
EN10_RS03215	1	18	-0.734854	VWA domain-containing protein
EN10_RS03220	1	18	-0.446649	hypothetical protein
EN10_RS03225	1	20	-0.542150	DUF58 domain-containing protein
EN10_RS03230	2	22	-0.587437	MoxR family ATPase
EN10_RS03235	2	22	-0.903795	uracil-DNA glycosylase
EN10_RS03240	0	21	-1.384748	LytR C-terminal domain-containing protein
EN10_RS03245	0	18	-1.960079	cold shock domain-containing protein
EN10_RS03250	0	18	-1.756231	chaperonin GroEL
EN10_RS03255	1	19	-2.490202	WXG100 family type VII secretion target
EN10_RS03260	1	19	-2.414080	hypothetical protein
EN10_RS03265	2	20	-2.152778	response regulator transcription factor
EN10_RS03270	1	15	-1.861508	HAMP domain-containing histidine kinase
EN10_RS03275	1	14	-1.073659	cold shock domain-containing protein
EN10_RS03280	1	13	-0.970542	DUF3027 domain-containing protein
EN10_RS03285	2	14	-0.447694	hypothetical protein
EN10_RS03290	2	14	-0.563838	universal stress protein
EN10_RS03295	2	13	-0.749039	ATP-dependent Clp protease ATP-binding subunit

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
EN10_RS03175	PHPHTRNFRASE	30	0.033	Phosphoenolpyruvate-protein phosphotransferase sign...
>PHPHTRNFRASE#Phosphoenolpyruvate-protein phosphotransferase signature. Length = 572 Score = 29.8 bits (67), Expect = 0.033 Identities = 26/185 (14%), Positives = 58/185 (31%), Gaps = 19/185 (10%) Query: 266 EAFALADP-IAANHAINTDGAHARIALAAGRSTTALELQQMMLEQVESFAAHHGDALEAS 324 +AF +P + TD + L A + EL+ + + S A + A Sbjct: 18 KAFIHLEPNVDIEKTSITDVSTEIEKLTAALEKSKEELRAIKDQTEASMGADKAEIFAA- 76 Query: 325 LRYDALCNVEWIVGQWRWVLDRLAANDIETLSHVVDWASKQVFFNRLQSRGTVTPARLRQ 384 + + + +V + IE ++A K+V + ++ +++ Sbjct: 77 --HLLVLDDPELVDGIK--------GKIENEQMNAEYALKEVSDMFVSMFESMDNEYMKE 126 Query: 385 LDLDYHDIANGRLYPSLCAHGLMRTLVDADQIHDAVSTPPPHTRAVLRGRFVA------- 437 D D++ L + + + + A P T + + Sbjct: 127 RAADIRDVSKRVLGHLIGVETGSLATIAEETVIIAEDLTPSDTAQLNKQFVKGFATDIGG 186 Query: 438 AASHT 442 SH+ Sbjct: 187 RTSHS 191
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
EN10_RS03180	DNABINDINGHU	91	7e-28	Prokaryotic integration host factor signature.
>DNABINDINGHU#Prokaryotic integration host factor signature. Length = 91 Score = 90.9 bits (226), Expect = 7e-28 Identities = 38/88 (43%), Positives = 53/88 (60%) Query: 4 NKSDLVAKISQKSGLTKAQAEAAVNAFRDVFIEAMKNGEGLKLTGLLSAERVKRAARTGR 63 NK DL+AK+++ + LTK + AAV+A + GE ++L G + E +RAAR GR Sbjct: 3 NKQDLIAKVAEATELTKKDSAAAVDAVFSAVSSYLAKGEKVQLIGFGNFEVRERAARKGR 62 Query: 64 NPRTGETIEIPATYGVRISAGSLLKKAV 91 NP+TGE I+I A+ AG LK AV Sbjct: 63 NPQTGEEIKIKASKVPAFKAGKALKDAV 90
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
EN10_RS03205	TYPE4SSCAGX	27	0.048	Type IV secretion system CagX conjugation protein si...
>TYPE4SSCAGX#Type IV secretion system CagX conjugation protein signature. Length = 522 Score = 27.4 bits (60), Expect = 0.048 Identities = 16/58 (27%), Positives = 30/58 (51%) Query: 106 STTYNAQVSKQLTDLVQQQVDADAAKNDSQQSGQHGEQPKSLDSLTEEQRKQVEELLR 163 S N +K L++L++QQ + + + + + Q Q +L + E +KQ EE +R Sbjct: 188 SNPQNLSNNKNLSELIKQQRENELDQMERLEDMQEQAQANALKQIEELNKKQAEEAVR 245
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
EN10_RS03230	HTHFIS	29	0.036	FIS bacterial regulatory protein HTH signature.
>HTHFIS#FIS bacterial regulatory protein HTH signature. Length = 484 Score = 28.6 bits (64), Expect = 0.036 Identities = 30/133 (22%), Positives = 52/133 (39%), Gaps = 16/133 (12%) Query: 68 HILIESVPGLAKTTAAQTL---ATSVSGSFKRVQC---TPDLMPSDLVGTQVFDFSTQRF 121 ++I G K A+ L +G F + DL+ S+L G + F T Sbjct: 162 TLMITGESGTGKELVARALHDYGKRRNGPFVAINMAAIPRDLIESELFGHEKGAF-TGAQ 220 Query: 122 TTQIGPI---HANFVLLDEINRANAKTQSAMLEAMAEGA-TTIGGQRIELPKPFMVIAT- 176 T G + LDEI Q+ +L + +G TT+GG+ + ++A Sbjct: 221 TRSTGRFEQAEGGTLFLDEIGDMPMDAQTRLLRVLQQGEYTTVGGRT-PIRSDVRIVAAT 279 Query: 177 -QNPIEE--EGTF 186 ++ + +G F Sbjct: 280 NKDLKQSINQGLF 292
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
EN10_RS03260	MECHCHANNEL	28	0.023	Bacterial mechano-sensitive ion channel signature.
>MECHCHANNEL#Bacterial mechano-sensitive ion channel signature. Length = 136 Score = 28.3 bits (63), Expect = 0.023 Identities = 17/78 (21%), Positives = 34/78 (43%), Gaps = 2/78 (2%) Query: 188 PLLDQQAGQGPNWSQSAIDMMDAIASAKSTATTHAVSSVSII-VMIIVFVVLIVVVLVMI 246 P L G G ++ Q A+ + DA + + V ++ +I+ F + + + L+ Sbjct: 43 PPLGLLIG-GIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINK 101 Query: 247 FMRRKREVSEGSKQTEEV 264 R+K E + T+E Sbjct: 102 LNRKKEEPAAAPAPTKEE 119
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
EN10_RS03265	HTHFIS	107	7e-29	FIS bacterial regulatory protein HTH signature.
>HTHFIS#FIS bacterial regulatory protein HTH signature. Length = 484 Score = 107 bits (268), Expect = 7e-29 Identities = 38/126 (30%), Positives = 67/126 (53%) Query: 5 TEATIVVVDDEPSIRELLVASLHFAGYEVETAASGSEAIEVISRTKPDMIILDVMLPDID 64 T ATI+V DD+ +IR +L +L AGY+V ++ + I+ D+++ DV++PD + Sbjct: 2 TGATILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDEN 61 Query: 65 GFTVTRRIRQEGITAPVLFLTARDDTQDKVMGLTVGGDDYVTKPFSLEEVVARIRAILRR 124 F + RI++ PVL ++A++ + G DY+ KPF L E++ I L Sbjct: 62 AFDLLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAE 121 Query: 125 TREQVE 130 + + Sbjct: 122 PKRRPS 127
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
EN10_RS03295	HTHFIS	43	6e-06	FIS bacterial regulatory protein HTH signature.
>HTHFIS#FIS bacterial regulatory protein HTH signature. Length = 484 Score = 42.5 bits (100), Expect = 6e-06 Identities = 35/160 (21%), Positives = 57/160 (35%), Gaps = 28/160 (17%) Query: 521 IIGQDEAVSALSRSIRRTRVGLKDPKRPAGSFIFAGPTGVGKTELAKALAQFLFDDEDAL 580 ++G+ A+ + R + R + + + G +G GK +A+AL + Sbjct: 139 LVGRSAAMQEIYRVLARL-------MQTDLTLMITGESGTGKELVARALHDYGKRRNGPF 191 Query: 581 IRVDMSEFSEKYAASRLFGAPPGYVGYEEGGELTEKVRRKP--FS-----VVLFDEIEKA 633 + ++M+ S LFG E G T R F + DEI Sbjct: 192 VAINMAAIPRDLIESELFGH--------EKGAFTGAQTRSTGRFEQAEGGTLFLDEIGDM 243 Query: 634 HPDIFNTLLQVLDDGHLT---DGQGRKVDFKNTIIILTTN 670 D LL+VL G T + D + I+ TN Sbjct: 244 PMDAQTRLLRVLQQGEYTTVGGRTPIRSDVR---IVAATN 280 Score = 31.3 bits (71), Expect = 0.019 Identities = 42/179 (23%), Positives = 69/179 (38%), Gaps = 32/179 (17%) Query: 163 QDKRSQSGSAILDQFGRNLTAEAKDGKLDPVIGRSTEIERVMVVLSRRTKNN-PVLI-GE 220 + G A+ + R E P++GRS ++ + VL+R + + ++I GE Sbjct: 109 TELIGIIGRALAEPKRRPSKLEDDSQDGMPLVGRSAAMQEIYRVLARLMQTDLTLMITGE 168 Query: 221 PGVGKTAVVEGLAQ----------KIVAGDVPETLKDKQVYSLDLGSMV-AGSRYRGDFE 269 G GK V L I +P L + +++ + G+ A +R G FE Sbjct: 169 SGTGKELVARALHDYGKRRNGPFVAINMAAIPRDLIESELFGHEKGAFTGAQTRSTGRFE 228 Query: 270 ERLKKVLKEIKTRGDIVLFIDEIHTIVGAGSADGALGASDMLKPMLARGELQTIGATTT 328 + LF+DEI D + A L +L +GE T+G T Sbjct: 229 Q-----------AEGGTLFLDEI--------GDMPMDAQTRLLRVLQQGEYTTVGGRTP 268

EN10_RS03895

EN10_RS03925

Genomic Island

LocusTag	DNBias	CDNBias	%GCBias	Product
EN10_RS03895	3	15	-2.076325	tryptophan synthase subunit alpha
EN10_RS03900	1	13	-3.054239	prolipoprotein diacylglyceryl transferase
EN10_RS03905	-1	15	-3.485587	ribulose-phosphate 3-epimerase
EN10_RS03910	-1	17	-3.968576	phosphoribosyl-ATP diphosphatase
EN10_RS03915	-1	18	-3.758317	ATP phosphoribosyltransferase
EN10_RS03920	-2	22	-3.693115	CDP-alcohol phosphatidyltransferase family
EN10_RS03925	-2	24	-3.102220	DUF881 domain-containing protein

EN10_RS04080

EN10_RS04190

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
EN10_RS04080	-1	13	-3.446594	threonine--tRNA ligase
EN10_RS04085	2	22	-4.078015	ATP-binding cassette domain-containing protein
EN10_RS04095	4	21	-3.359125	*GNAT family N-acetyltransferase
EN10_RS04100	3	17	-2.517797	hypothetical protein
EN10_RS04105	3	18	-2.000328	hypothetical protein
EN10_RS04135	2	17	-1.212012	****PAC2 family protein
EN10_RS04140	2	18	-0.639719	undecaprenyl-diphosphate phosphatase
EN10_RS04145	2	17	-0.829546	fructosamine kinase family protein
EN10_RS04150	1	15	-1.461843	molecular chaperone DnaJ
EN10_RS04155	2	17	-1.647648	heat-inducible transcriptional repressor HrcA
EN10_RS04160	1	16	-2.898558	transketolase
EN10_RS04165	-2	14	-4.938431	transaldolase
EN10_RS04170	-2	15	-5.883333	hypothetical protein
EN10_RS04175	-2	16	-5.762396	aminotransferase class I/II-fold pyridoxal
EN10_RS04180	-1	16	-4.957146	Lrp/AsnC family transcriptional regulator
EN10_RS04185	-1	19	-4.462154	aminotransferase class I/II-fold pyridoxal
EN10_RS04190	0	22	-3.936080	HAD hydrolase family protein

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
EN10_RS04085	PF03627	27	0.038	PapG
>PF03627#PapG Length = 336 Score = 27.2 bits (60), Expect = 0.038 Identities = 15/49 (30%), Positives = 27/49 (55%), Gaps = 5/49 (10%) Query: 17 SSEPLFDH---FNASLSHGWTALLADNGL--GKTTLAQLICGRRTPTRG 60 ++ P + H F+ L HGW ++++ NG+ G+TT+ G + T G Sbjct: 262 NTTPTYSHGKKFSVGLGHGWDSIVSVNGVDTGETTMRWYKAGTQNLTIG 310
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
EN10_RS04095	SACTRNSFRASE	48	1e-09	Streptothricin acetyltransferase signature.
>SACTRNSFRASE#Streptothricin acetyltransferase signature. Length = 173 Score = 48.0 bits (114), Expect = 1e-09 Identities = 19/95 (20%), Positives = 41/95 (43%), Gaps = 7/95 (7%) Query: 61 FVATVDGVPAGYMKVNLGTAQTERMPDDHAEVQRLYVLADFKRHGLGTRFMRTAEQFASA 120 F+ ++ G +K+ + +A ++ + V D+++ G+GT + A ++A Sbjct: 68 FLYYLENNCIGRIKIRSNW-------NGYALIEDIAVAKDYRKKGVGTALLHKAIEWAKE 120 Query: 121 HGKQTLWLGVWEHNEPAKAFYRRMGFVETGAHTFV 155 + L L + N A FY + F+ T + Sbjct: 121 NHFCGLMLETQDINISACHFYAKHHFIIGAVDTML 155

EN10_RS04245

EN10_RS04300

Genomic Island

LocusTag	DNBias	CDNBias	%GCBias	Product
EN10_RS04245	-1	15	-3.267411	DNA-deoxyinosine glycosylase
EN10_RS04250	0	17	-2.321251	alpha/beta hydrolase-fold protein
EN10_RS04255	-1	16	-2.380535	ATP-binding cassette domain-containing protein
EN10_RS04260	-1	16	-2.480149	cation transporter
EN10_RS04265	-2	13	-2.762190	carboxymuconolactone decarboxylase family
EN10_RS04270	-2	11	-2.369513	choloylglycine hydrolase
EN10_RS04275	-1	16	-2.384730	bifunctional ADP-dependent NAD(P)H-hydrate
EN10_RS04280	-2	15	-3.425512	alkyl hydroperoxide reductase subunit C
EN10_RS04285	-3	17	-3.479996	FAD-dependent oxidoreductase
EN10_RS04290	-1	20	-4.866811	LysR family transcriptional regulator
EN10_RS04295	-1	22	-3.693279	HAD family phosphatase
EN10_RS04300	0	21	-3.002908	hypothetical protein

EN10_RS04565

EN10_RS04590

Genomic Island

LocusTag	DNBias	CDNBias	%GCBias	Product
EN10_RS04565	0	22	-3.531902	pullulanase
EN10_RS04575	-1	33	-4.257225	pullulanase
EN10_RS04580	-2	34	-4.454856	alpha-amylase family glycosyl hydrolase
EN10_RS08395	-2	37	-5.134747	hypothetical protein
EN10_RS04585	-3	34	-5.162171	hypothetical protein
EN10_RS04590	-2	29	-4.506313	hypothetical protein

EN10_RS04690

EN10_RS04855

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
EN10_RS04690	1	21	-3.209484	DUF3737 family protein
EN10_RS04700	3	23	-3.174484	aminotransferase class I/II-fold pyridoxal
EN10_RS04705	3	23	-3.571187	hypothetical protein
EN10_RS04710	2	23	-3.585729	alpha-amylase
EN10_RS04715	3	21	-3.702152	gamma-glutamyl-gamma-aminobutyrate hydrolase
EN10_RS04720	1	19	-3.205228	prolyl-tRNA synthetase associated
EN10_RS04725	2	19	-4.035607	ABC-2 transporter permease
EN10_RS04730	2	19	-3.997491	ABC transporter ATP-binding protein
EN10_RS04735	2	24	-4.035317	GntR family transcriptional regulator
EN10_RS04740	3	24	-4.037049	helix-turn-helix domain-containing protein
EN10_RS04745	3	30	-3.929653	adenosine deaminase
EN10_RS04750	2	33	-4.876027	hypothetical protein
EN10_RS04755	3	36	-5.594386	chorismate mutase
EN10_RS04760	5	38	-6.273178	hypothetical protein
EN10_RS04765	8	38	-7.057406	GNAT family N-acetyltransferase
EN10_RS04770	5	29	-5.288437	adenylate kinase
EN10_RS04775	5	18	-3.634804	DUF4143 domain-containing protein
EN10_RS04780	4	16	-3.160071	tetracycline resistance ribosomal protection
EN10_RS04785	3	17	-1.561705	IS5-like element ISBian1 family transposase
EN10_RS04790	1	16	-1.029776	hypothetical protein
EN10_RS04795	0	15	-1.132304	glutamine-hydrolyzing GMP synthase
EN10_RS04800	-1	14	-2.312056	phosphoketolase
EN10_RS04805	-1	14	-3.304150	phosphate acetyltransferase
EN10_RS04810	1	14	-3.358059	acetate kinase
EN10_RS04815	2	18	-3.429608	3-phosphoshikimate 1-carboxyvinyltransferase
EN10_RS04825	1	16	-3.374889	*hypothetical protein
EN10_RS04830	1	15	-3.110329	glycogen debranching protein GlgX
EN10_RS04835	0	14	-1.681011	NUDIX hydrolase
EN10_RS04840	-1	13	-1.876251	Nif3-like dinuclear metal center hexameric
EN10_RS04845	-1	12	-1.717030	DNA polymerase I
EN10_RS04850	-1	13	-1.408689	response regulator
EN10_RS04855	2	18	-1.213283	NUDIX hydrolase

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
EN10_RS04735	UREASE	28	0.015	Urea amidohydrolase (urease) protein signature.
>UREASE#Urea amidohydrolase (urease) protein signature. Length = 570 Score = 27.8 bits (62), Expect = 0.015 Identities = 20/70 (28%), Positives = 29/70 (41%), Gaps = 16/70 (22%) Query: 25 IKEGRL----KAGEP--LDSVRSLARTCRISALTVKKAYDVLEREGLVVTVQGKGSFVAQ 78 +K+GR+ KAG P V + V +V+ EG +VT G S + Sbjct: 90 LKDGRIAAIGKAGNPDMQPGV----------TIIVGPGTEVIAGEGKIVTAGGMDSHIHF 139 Query: 79 ISPNIVAEEL 88 I P + E L Sbjct: 140 ICPQQIEEAL 149
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
EN10_RS04765	SACTRNSFRASE	37	6e-06	Streptothricin acetyltransferase signature.
>SACTRNSFRASE#Streptothricin acetyltransferase signature. Length = 173 Score = 36.8 bits (85), Expect = 6e-06 Identities = 16/58 (27%), Positives = 25/58 (43%) Query: 59 YAFVENVVTRADARGHGYATACLNHAKALAQQAGCYKMMLLTGSHDPKTLDFYRHAGY 116 YA +E++ D R G TA L+ A A++ +ML T + FY + Sbjct: 89 YALIEDIAVAKDYRKKGVGTALLHKAIEWAKENHFCGLMLETQDINISACHFYAKHHF 146
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
EN10_RS04780	TCRTETOQM	1016	0.0	Tetracycline resistance protein TetO/TetQ/TetM family ...
>TCRTETOQM#Tetracycline resistance protein TetO/TetQ/TetM family signature. Length = 639 Score = 1016 bits (2628), Expect = 0.0 Identities = 437/639 (68%), Positives = 522/639 (81%) Query: 1 MKIINIGILAHVDAGKTTLTESLLYASGAISEPGSVEKGTTRTDTMLLERQRGITIQAAV 60 MKIINIG+LAHVDAGKTTLTESLLY SGAI+E GSV+KGTTRTD LLERQRGITIQ + Sbjct: 1 MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTDNTLLERQRGITIQTGI 60 Query: 61 TSFQWHRCKVNIVDTPGHMDFLAEVYRSLAVLDGAILVISAKDGVQAQTRILFHALRKMN 120 TSFQW KVNI+DTPGHMDFLAEVYRSL+VLDGAIL+ISAKDGVQAQTRILFHALRKM Sbjct: 61 TSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAKDGVQAQTRILFHALRKMG 120 Query: 121 IPTVIFINKIDQAGVDLQSVVQSVRDKLSADIIIKQTVSLSPEIVLEENTDIEAWDAVIE 180 IPT+ FINKIDQ G+DL +V Q +++KLSA+I+IKQ V L P + + T+ E WD VIE Sbjct: 121 IPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVELYPNMCVTNFTESEQWDTVIE 180 Query: 181 NNDKLLEKYIAGEPISREKLVREEQRRVQDASLFPVYYGSAKKGLGIQPLMDAVTGLFQP 240 ND LLEKY++G+ + +L +EE R + SLFPVY+GSAK +GI L++ +T F Sbjct: 181 GNDDLLEKYMSGKSLEALELEQEESIRFHNCSLFPVYHGSAKNNIGIDNLIEVITNKFYS 240 Query: 241 IGEQGSAALCGSVFKVEYTDCGQRRVYLRLYSGTLRLRDTVALAGREKLKITEMRIPSKG 300 +G + LCG VFK+EY++ QR Y+RLYSG L LRD+V ++ +EK+KITEM G Sbjct: 241 STHRGQSELCGKVFKIEYSEKRQRLAYIRLYSGVLHLRDSVRISEKEKIKITEMYTSING 300 Query: 301 EIVRTDTAYPGEIVILPSDSVRLNDVLGDPTRLPRKRWREDPLPMLRTSIAPKTAAQRER 360 E+ + D AY GEIVIL ++ ++LN VLGD LP++ E+PLP+L+T++ P QRE Sbjct: 301 ELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLPQRERIENPLPLLQTTVEPSKPQQREM 360 Query: 361 LLDALTQLADTDPLLRCEVDSITHEIILSFLGRVQLEVVSALLSEKYKLETVVKEPTVIY 420 LLDAL +++D+DPLLR VDS THEIILSFLG+VQ+EV ALL EKY +E +KEPTVIY Sbjct: 361 LLDALLEISDSDPLLRYYVDSATHEIILSFLGKVQMEVTCALLQEKYHVEIEIKEPTVIY 420 Query: 421 MERPLKAASHTIHIEVPPNPFWASIGLSVTPLPLGSGVQYKSRVSLGYLNQSFQNAVRDG 480 MERPLK A +TIHIEVPPNPFWASIGLSV+PLPLGSG+QY+S VSLGYLNQSFQNAV +G Sbjct: 421 MERPLKKAEYTIHIEVPPNPFWASIGLSVSPLPLGSGMQYESSVSLGYLNQSFQNAVMEG 480 Query: 481 IRYGLEQGLFGWNVTDCKICFEYGLYYSPVSTPADFRSLAPIVLEQALKESGTQLLEPYL 540 IRYG EQGL+GWNVTDCKICF+YGLYYSPVSTPADFR LAPIVLEQ LK++GT+LLEPYL Sbjct: 481 IRYGCEQGLYGWNVTDCKICFKYGLYYSPVSTPADFRMLAPIVLEQVLKKAGTELLEPYL 540 Query: 541 SFTLYAPREYLSRAYHDAPKYCATIETVQVKKDEVVFTGEIPARCIQAYRTDLAFYTNGQ 600 SF +YAP+EYLSRAY DAPKYCA I Q+K +EV+ +GEIPARCIQ YR+DL F+TNG+ Sbjct: 541 SFKIYAPQEYLSRAYTDAPKYCANIVDTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGR 600 Query: 601 SVCLTELKGYQAAVGKPVIQPRRPNSRLDKVRHMFSKIT 639 SVCLTELKGY G+PV QPRRPNSR+DKVR+MF+KIT Sbjct: 601 SVCLTELKGYHVTTGEPVCQPRRPNSRIDKVRYMFNKIT 639
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
EN10_RS04805	PilS_PF08805	29	0.027	PilS N terminal
>PilS_PF08805#PilS N terminal Length = 185 Score = 29.1 bits (65), Expect = 0.027 Identities = 9/53 (16%), Positives = 19/53 (35%) Query: 314 MVPKLCELRAKKGMTPDVALKTLRDTNYFGTMLIVLGMADGLVSGAISSTANT 366 M L A++ D + G ++++ A L S S+ ++ Sbjct: 9 MRSVFSSLSARRKKEQDKGATLMEVLLVVGVIVVLAASAYKLYSMVQSNIQSS 61
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
EN10_RS04810	ACETATEKNASE	422	e-149	Acetate kinase family signature.
>ACETATEKNASE#Acetate kinase family signature. Length = 400 Score = 422 bits (1086), Expect = e-149 Identities = 167/400 (41%), Positives = 250/400 (62%), Gaps = 9/400 (2%) Query: 5 VLVINSGSSSIKYQLVDLESGEGIASGIVEKIGEPIDGHYKYEYNGEKHELDEKVENHEQ 64 +LVIN GSSS+KYQL++ + G +A G+ E+IG D + NGEK ++ + +++H+ Sbjct: 3 ILVINCGSSSLKYQLIESKDGNVLAKGLAERIGIN-DSLLTHNANGEKIKIKKDMKDHKD 61 Query: 65 GLKRVLGFF--EEYGPSLEETGIIAVGHRVVQGGDTFPNPALVTPDTTAKVEELAVLAPL 122 +K VL +YG + + I AVGHRVV GG+ F + L+T D + + LAPL Sbjct: 62 AIKLVLDALVNSDYGVIKDMSEIDAVGHRVVHGGEYFTSSVLITDDVLKAITDCIELAPL 121 Query: 123 HNGPEAKGARVMRELLPEIPQVYVFDSSFFQQLPKASSTYALNKEVADKYKIKRYGAHGT 182 HN +G + +++P++P V VFD++F Q +P + Y + E KYKI++YG HGT Sbjct: 122 HNPANIEGIKACTQIMPDVPMVAVFDTAFHQTMPDYAYLYPIPYEYYTKYKIRKYGFHGT 181 Query: 183 SHWFVSSQIPEFLGIDAEAAKGLKQIVLHIGNGASASAELGGRPIETSMGLTPLEGLVMG 242 SH +VS + E L E LK I H+GNG+S +A G+ I+TSMG TPLEGL MG Sbjct: 182 SHKYVSQRAAEILNKPIE---SLKIITCHLGNGSSIAAVKNGKSIDTSMGFTPLEGLAMG 238 Query: 243 SRTGDIDPAAVFHLMRNADMNVDELDDLFNKKSGMMGMTGF-GDMREV-HRLVAEGDENA 300 +R+G IDP+ + +LM +++ +E+ ++ NKKSG+ G++G D R++ GD+ A Sbjct: 239 TRSGSIDPSIISYLMEKENISAEEVVNILNKKSGVYGISGISSDFRDLEDAAFKNGDKRA 298 Query: 301 KLALDIYVHRIVSYIGAYTAQLGGLDVLTFTAGVGENDEIVRRMVCEKLAPFGVKLDNDK 360 +LAL+++ +R+ IG+Y A +GG+DV+ FTAG+GEN +R + + L G KLD +K Sbjct: 299 QLALNVFAYRVKKTIGSYAAAMGGVDVIVFTAGIGENGPEIREFILDGLEFLGFKLDKEK 358 Query: 361 NDTRSKEPRIISTPDSTVTVVVYPTNEELAIAQRANEIVT 400 N R +E IIST DS V V+V PTNEE IA+ +IV Sbjct: 359 NKVRGEE-AIISTADSKVNVMVVPTNEEYMIAKDTEKIVE 397
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
EN10_RS04850	HTHFIS	76	6e-18	FIS bacterial regulatory protein HTH signature.
>HTHFIS#FIS bacterial regulatory protein HTH signature. Length = 484 Score = 76.0 bits (187), Expect = 6e-18 Identities = 34/171 (19%), Positives = 66/171 (38%), Gaps = 23/171 (13%) Query: 38 PTVVVAEDESVNRMDLVAMLEDNGYEVVGEAANGEEAIELTREKRPDVVCMDVKMPHMDG 97 T++VA+D++ R L L GY+V +N D+V DV MP + Sbjct: 4 ATILVADDDAAIRTVLNQALSRAGYDVRI-TSNAATLWRWIAAGDGDLVVTDVVMPDENA 62 Query: 98 ITAAGVICDENL-APVVMLTAFSQADLVKEAIGAGAMAYVTKPYEESKLLPALAVAMGRF 156 I PV++++A + +A GA Y+ KP+ Sbjct: 63 FDLLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPF---------------- 106 Query: 157 AEINDLLDSVERNESELRTTQEQLRDAEEELR----KTKETLEERKLIDRA 203 ++ +L+ + R +E + +L D ++ ++ E +++ R Sbjct: 107 -DLTELIGIIGRALAEPKRRPSKLEDDSQDGMPLVGRSAAMQEIYRVLARL 156

EN10_RS04930

EN10_RS04995

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
EN10_RS04930	2	26	-2.668274	HAMP domain-containing histidine kinase
EN10_RS04935	2	25	-2.762256	ComF family protein
EN10_RS04940	2	23	-2.716747	HAD-IIB family hydrolase
EN10_RS04945	2	25	-2.648439	metallopeptidase family protein
EN10_RS04950	2	24	-2.686217	DUF5719 family protein
EN10_RS04955	2	22	-2.827204	glycosyltransferase family 2 protein
EN10_RS04960	2	18	-2.550944	LCP family protein
EN10_RS04965	3	20	-2.519786	LCP family protein
EN10_RS04970	3	19	-2.308098	hypothetical protein
EN10_RS04975	2	16	-1.943776	WhiB family transcriptional regulator
EN10_RS04980	3	15	-1.968378	PAS domain-containing sensor histidine kinase
EN10_RS04985	2	15	-1.316018	hemolysin III family protein
EN10_RS04990	2	17	-1.428124	transcription elongation factor GreA
EN10_RS04995	2	17	-1.194216	FKBP-type peptidyl-prolyl cis-trans isomerase

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
EN10_RS04980	PF06580	55	2e-10	Sensor histidine kinase
>PF06580#Sensor histidine kinase Length = 349 Score = 55.3 bits (133), Expect = 2e-10 Identities = 34/223 (15%), Positives = 81/223 (36%), Gaps = 37/223 (16%) Query: 275 ELRRREVELQTKDATISEIHHRVK-----NNLQAVSALLRLQARKTKSQEVKKELQEAMR 329 E+ + ++ ++A + + ++ N L + AL+ TK++E+ L E MR Sbjct: 148 EIDQWKMASMAQEAQLMALKAQINPHFMFNALNNIRALILEDP--TKAREMLTSLSELMR 205 Query: 330 RVQTIAMVHEGLSQTADENVDFDKVISNLLRMSVDLASMR-----DQHITIDFVGKFGKM 384 L + V + ++ + LAS++ I+ Sbjct: 206 YS---------LRYSNARQVSLADEL-TVVDSYLQLASIQFEDRLQFENQINP------- 248 Query: 385 PAQDATPLSLVLTELITNAVEHGYDGRKE-GHITVSVGREGKCLNVVVEDDGNGMDQEES 443 D +++ L+ N ++HG + G I + ++ + + VE+ G+ + Sbjct: 249 AIMDVQVPPMLVQTLVENGIKHGIAQLPQGGKILLKGTKDNGTVTLEVENTGSLALKNTK 308 Query: 444 NGMAKASGSGLGTQIINTFVTNDFGGNVRWEHG-RDGGTRVIL 485 +G G Q + + +G + + + G ++ Sbjct: 309 E------STGTGLQNVRERLQMLYGTEAQIKLSEKQGKVNAMV 345
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
EN10_RS04995	INFPOTNTIATR	60	5e-14	Macrophage infectivity potentiator signature.
>INFPOTNTIATR#Macrophage infectivity potentiator signature. Length = 233 Score = 60.0 bits (145), Expect = 5e-14 Identities = 38/112 (33%), Positives = 57/112 (50%), Gaps = 8/112 (7%) Query: 26 PQGLKVVELEEGNGPIVRRGDTVTVNYHGQVWGNDKAFDSSFERHHPASFGIGVGQVIKG 85 P GL+ ++ G G + DTVTV Y G + + FDS+ + PA+F V QVI G Sbjct: 125 PSGLQYKIIDAGTGAKPGKSDTVTVEYTGTLI-DGTVFDSTEKAGKPATF--QVSQVIPG 181 Query: 86 WDQTVPGHNVGSRLLISIPPQYGYGAN--GAPQAGIGGDDTLVFVVDIISTR 135 W + + GS + +P YG G P IG ++TL+F + +IS + Sbjct: 182 WTEALQLMPAGSTWEVFVPADLAYGPRSVGGP---IGPNETLIFKIHLISVK 230

EN10_RS05225

EN10_RS05265

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
EN10_RS05225	2	12	-0.360982	histidine kinase
EN10_RS05230	1	11	-0.480989	response regulator transcription factor
EN10_RS05235	1	10	-0.437609	FtsX-like permease family protein
EN10_RS05240	2	14	-1.309149	ABC transporter ATP-binding protein
EN10_RS05245	3	16	-1.222362	energy-coupling factor transporter transmembrane
EN10_RS05250	2	15	-0.536602	ABC transporter ATP-binding protein
EN10_RS05255	1	17	-0.081288	O-acetylhomoserine
EN10_RS05260	2	18	0.187641	pyridoxamine kinase
EN10_RS05265	2	19	0.051158	YraN family protein

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
EN10_RS05230	HTHFIS	61	7e-13	FIS bacterial regulatory protein HTH signature.
>HTHFIS#FIS bacterial regulatory protein HTH signature. Length = 484 Score = 60.6 bits (147), Expect = 7e-13 Identities = 21/121 (17%), Positives = 44/121 (36%), Gaps = 5/121 (4%) Query: 5 SRIRMVIADDQELVRAGFTMVINSQPDMQVVGQASDGTQAVQLVERLHPDIVLMDVRMPS 64 + +++ADD +R ++ V S+ + + D+V+ DV MP Sbjct: 2 TGATILVADDDAAIRTVLNQALSRA-GYDVRI-TSNAATLWRWIAAGDGDLVVTDVVMPD 59 Query: 65 MDGLAATAQITSHADGEQHTRVIILTTFDLDEYVMSAINAGASGFLLKDTEPETLLNSIR 124 + +I V++++ + + A GA +L K + L+ I Sbjct: 60 ENAFDLLPRIKKA---RPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIG 116 Query: 125 T 125 Sbjct: 117 R 117
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
EN10_RS05245	BCTERIALGSPF	28	0.041	Bacterial general secretion pathway protein F signa...
>BCTERIALGSPF#Bacterial general secretion pathway protein F signature. Length = 408 Score = 27.9 bits (62), Expect = 0.041 Identities = 7/39 (17%), Positives = 15/39 (38%) Query: 35 WGNWVFLTAMLLAEHLFLLSTRIPARKTLKVWSFFLPVI 73 +G W+ L + ++ + R + LP+I Sbjct: 226 FGPWMLLALLAGFMAFRVMLRQEKRRVSFHRRLLHLPLI 264

EN10_RS05480

EN10_RS05540

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
EN10_RS05480	2	15	0.426848	1-acyl-sn-glycerol-3-phosphate acyltransferase
EN10_RS05485	3	16	0.509187	TrbC/VirB2 family protein
EN10_RS05490	2	16	0.598697	anthranilate phosphoribosyltransferase
EN10_RS05495	3	18	0.272318	preprotein translocase subunit SecA
EN10_RS05500	2	19	-0.347176	ribosome-associated translation inhibitor RaiA
EN10_RS05505	0	17	-0.996463	recombination regulator RecX
EN10_RS05510	-1	13	-0.110427	recombinase RecA
EN10_RS05515	-1	13	0.325761	DUF3046 domain-containing protein
EN10_RS05520	-1	11	0.474167	helix-turn-helix domain-containing protein
EN10_RS05525	-1	12	0.056101	nicotinamide-nucleotide amidohydrolase family
EN10_RS05530	1	11	0.141784	CDP-diacylglycerol--glycerol-3-phosphate
EN10_RS05535	2	11	0.285437	DNA translocase FtsK
EN10_RS05540	2	11	-0.344848	tRNA (adenosine(37)-N6)-dimethylallyltransferase

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
EN10_RS05495	SECA	1119	0.0	SecA protein signature.
>SECA#SecA protein signature. Length = 901 Score = 1119 bits (2897), Expect = 0.0 Identities = 432/992 (43%), Positives = 591/992 (59%), Gaps = 111/992 (11%) Query: 1 MVDIVDKALRMGEGRQIKKLEHVAEAVNKLEDQMVVMSDDELKGQTAKFKERLANGETLD 60 ++ ++ K R ++++ V +N +E +M +SD+ELKG+TA+F+ RL GE L+ Sbjct: 2 LIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELKGKTAEFRARLEKGEVLE 61 Query: 61 DLMPEAFATVREVSKRTLGQRHFDVQLMGGAALHWGNIAEMKTGEGKTLVATLPSYLNAL 120 +L+PEAFA VRE SKR G RHFDVQL+GG L+ IAEM+TGEGKTL ATLP+YLNAL Sbjct: 62 NLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEMRTGEGKTLTATLPAYLNAL 121 Query: 121 EGKGVHVITVNDYLASYQSELMGRIYRFLGMSVGCIVTGQKPAERRKQYNADITYGTNNE 180 GKGVHV+TVNDYLA +E ++ FLG++VG + G +R+ Y ADITYGTNNE Sbjct: 122 TGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPGMPAPAKREAYAADITYGTNNE 181 Query: 181 FGFDYLRDNMAWEKNELVQRGHHYAIVDEVDSILIDEARTPLIISGPAEGDVTRWYRQFA 240 +GFDYLRDNMA+ E VQR HYA+VDEVDSILIDEARTPLIISGPAE + Y++ Sbjct: 182 YGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDEARTPLIISGPAEDS-SEMYKRVN 240 Query: 241 KLVLKLNR-----------DEDYEVDEKKKTVGILDPGITKIEDYLGI-------DNLYE 282 K++ L R + + VDEK + V + + G+ IE+ L ++LY Sbjct: 241 KIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTERGLVLIEELLVKEGIMDEGESLYS 300 Query: 283 PSNTALIGYLNNAIKAKELFLRDRDYVVTGGEVLIVDEHTGRILPGRRYNEGLHQAIEAK 342 P+N L+ ++ A++A LF RD DY+V GEV+IVDEHTGR + GRR+++GLHQA+EAK Sbjct: 301 PANIMLMHHVTAALRAHALFTRDVDYIVKDGEVIIVDEHTGRTMQGRRWSDGLHQAVEAK 360 Query: 343 ENVEVKAENQTFATITLQNYFRMYDKLAGMTGTAETEAAEFMGTYKLGVLPIPPNKPMIR 402 E V+++ ENQT A+IT QNYFR+Y+KLAGMTGTA+TEA EF YKL + +P N+PMIR Sbjct: 361 EGVQIQNENQTLASITFQNYFRLYEKLAGMTGTADTEAFEFSSIYKLDTVVVPTNRPMIR 420 Query: 403 IDQDDLIFRTKKEKLAAIVKDVAARHRKGQPVLLGTASVESSEVVSSLLDVVEIPHKVLN 462 D DL++ T+ EK+ AI++D+ R KGQPVL+GT S+E SE+VS+ L I H VLN Sbjct: 421 KDLPDLVYMTEAEKIQAIIEDIKERTAKGQPVLVGTISIEKSELVSNELTKAGIKHNVLN 480 Query: 463 AKQHEKEAAVVAVAGRKGAVTVATNMAGRGTDIMLGGNVEFLADAELKAKGYSPDDTPEE 522 AK H EAA+VA AG AVT+ATNMAGRGTDI+LGG+ + A Sbjct: 481 AKFHANEAAIVAQAGYPAAVTIATNMAGRGTDIVLGGSWQAEVAAL-------------- 526 Query: 523 YEKLWPETLKKIKEQVKDEHEEVKKLGGLYVLGTERHESRRIDNQLRGRSGRQGDPGESR 582 E E ++KIK + H+ V + GGL+++GTERHESRRIDNQLRGRSGRQGD G SR Sbjct: 527 -ENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRRIDNQLRGRSGRQGDAGSSR 585 Query: 583 FYLSLEDDLMRLFNTQLVARVMAK-GMPEGEPIESKSVSKGVRNAQKAVESRNFEIRKNV 641 FYLS+ED LMR+F + V+ +M K GM GE IE V+K + NAQ+ VESRNF+IRK + Sbjct: 586 FYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVTKAIANAQRKVESRNFDIRKQL 645 Query: 642 LKYDDVMNKQRTVIYSERQAVLKGEDIHEDIEAFISDTLTSYVRGAKNGSDKPADWDWNG 701 L+YDDV N QR IYS+R +L D+ E I + D + + WD G Sbjct: 646 LEYDDVANDQRRAIYSQRNELLDVSDVSETINSIREDVFKATIDAYIPPQSLEEMWDIPG 705 Query: 702 LFKAVNDLYPTKVTIDEAKEAAEGLKGDKAVDAVVKLFVDDAEAQYEAFETKLGADGLRT 761 L + + + + + I E + L + + + + + Y+ E +GA+ +R Sbjct: 706 LQERLKNDFDLDLPIAEWLDKEPELHEETLRERI----LAQSIEVYQRKEEVVGAEMMRH 761 Query: 762 LERRVVLAVLDRKWREHLYEMDYLKDGIGLRGMGQRDPLVEYQREGYQMYNQMIEAIKEE 821 E+ V+L LD W+EHL MDYL+ GI LRG Q+DP EY+RE + M+ M+E++K E Sbjct: 762 FEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKDPKQEYKRESFSMFAAMLESLKYE 821 Query: 822 TVQLLFHIDLDSIAQTNDDGTDSIDDAAVDSAEIKMGDDVSEDDELNKGNLSEHEPEEAA 881 + L + +++M ++V E ++ + Sbjct: 822 VISTLSKV------------------------QVRMPEEVEELEQQRR------------ 845 Query: 882 RIDNHADELETAENIAAVKEAAEEGERIPESGLLGPEPMSHAEGKVPARKRPKSEELKTP 941 EA + + +SH + A + +T Sbjct: 846 ------------------MEAERLAQM---------QQLSHQDDDSAA---AAALAAQTG 875 Query: 942 WSDGRTFPGTPKNAPCPCGSGRKYKMCHGQNE 973 +N PCPCGSG+KYK CHG+ + Sbjct: 876 E------RKVGRNDPCPCGSGKKYKQCHGRLQ 901
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
EN10_RS05535	PF05616	31	0.025	Neisseria meningitidis TspB protein
>PF05616#Neisseria meningitidis TspB protein Length = 501 Score = 30.9 bits (69), Expect = 0.025 Identities = 32/108 (29%), Positives = 47/108 (43%), Gaps = 19/108 (17%) Query: 390 ETFRQFKVDAKVVGFLRGPSVTQY--EVEVAPGVKVE--KVTNLDKNIAYAVA------- 438 E KVDA +++ Y +VEVAPG KV VT+ + N VA Sbjct: 241 EEILSLKVDANPDKYIKATGYPGYSEKVEVAPGTKVNMGPVTDRNGNPVQVVATFGRDSQ 300 Query: 439 ---SSDVRILSPIPGKSAIGIEIPNADREIVHLGDVLRSDKAMNDPNP 483 + DV+++ P P + E PNA L +V ++ N+P P Sbjct: 301 GNTTVDVQVI-PRPDLTPGSAEAPNAQP----LPEVSPAENPANNPAP 343

EN10_RS05835

EN10_RS05880

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
EN10_RS05835	4	27	-1.738960	D-aminoacyl-tRNA deacylase
EN10_RS05840	6	31	-1.439567	site-specific integrase
EN10_RS05845	7	41	-2.441829	hypothetical protein
EN10_RS05850	8	48	0.549422	helix-turn-helix domain-containing protein
EN10_RS05855	9	51	-0.138126	helix-turn-helix domain-containing protein
EN10_RS05860	9	51	-0.019959	hypothetical protein
EN10_RS05865	9	52	-0.457404	hypothetical protein
EN10_RS05870	11	54	-0.531182	hypothetical protein
EN10_RS05875	7	32	-1.503979	AAA family ATPase
EN10_RS05880	9	30	-2.552378	hypothetical protein

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
EN10_RS05850	HTHTETR	24	0.045	TetR bacterial regulatory protein HTH signature.
>HTHTETR#TetR bacterial regulatory protein HTH signature. Length = 215 Score = 24.2 bits (52), Expect = 0.045 Identities = 6/46 (13%), Positives = 19/46 (41%), Gaps = 1/46 (2%) Query: 16 IKQVAERIGMSATTIYHKRRTHKDEVPPAIRIGRKLFWRPKDVDQW 61 + ++A+ G++ IY + K ++ I + +++ Sbjct: 34 LGEIAKAAGVTRGAIYWHFKD-KSDLFSEIWELSESNIGELELEYQ 78

EN10_RS06395

EN10_RS06460

Genomic Island

LocusTag	DNBias	CDNBias	%GCBias	Product
EN10_RS06395	2	20	0.918243	mupirocin-resistant isoleucine--tRNA ligase
EN10_RS08465	3	27	0.387436	hypothetical protein
EN10_RS06400	2	25	0.331670	tyrosine-protein phosphatase
EN10_RS06405	3	26	0.044721	hypothetical protein
EN10_RS06410	3	26	0.323156	hypothetical protein
EN10_RS06415	2	25	0.431024	ABC transporter ATP-binding protein/permease
EN10_RS06420	1	19	-0.698884	ABC transporter ATP-binding protein/permease
EN10_RS06425	-2	29	-3.865082	MarR family winged helix-turn-helix
EN10_RS06430	1	52	-8.114551	NADPH-dependent FMN reductase
EN10_RS06435	4	62	-9.762525	very short patch repair endonuclease
EN10_RS06440	5	66	-10.702169	antitoxin VbhA family protein
EN10_RS08290	5	67	-11.134470	helix-turn-helix transcriptional regulator
EN10_RS08295	4	68	-11.574820	hypothetical protein
EN10_RS06450	3	57	-9.741786	CRISPR-associated protein
EN10_RS06455	0	23	-4.297274	type I-U CRISPR-associated protein Csb2
EN10_RS06460	-1	16	-3.024039	type I-U CRISPR-associated RAMP protein

EN10_RS06630

EN10_RS06670

Genomic Island

LocusTag	DNBias	CDNBias	%GCBias	Product
EN10_RS06630	2	22	2.924774	hypothetical protein
EN10_RS06635	2	22	2.844748	FHA domain-containing protein
EN10_RS06640	2	24	2.908624	DNA-directed RNA polymerase subunit beta'
EN10_RS06645	1	22	2.921595	DNA-directed RNA polymerase subunit beta
EN10_RS06650	1	19	2.760149	hypothetical protein
EN10_RS06655	2	18	3.143500	A/G-specific adenine glycosylase
EN10_RS06660	2	22	2.900963	tRNA (cytidine(34)-2'-O)-methyltransferase
EN10_RS06665	4	23	2.748658	PFL family protein
EN10_RS06670	2	19	2.595534	ACT domain-containing protein

EN10_RS06715

EN10_RS06990

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
EN10_RS06715	1	20	3.171770	Crp/Fnr family transcriptional regulator
EN10_RS06720	1	20	3.169222	lipoate--protein ligase family protein
EN10_RS06725	1	20	3.138412	NINE protein
EN10_RS06730	-1	18	3.312345	3-isopropylmalate dehydrogenase
EN10_RS06735	-1	20	3.252493	S9 family peptidase
EN10_RS06740	-2	17	2.522126	DUF2183 domain-containing protein
EN10_RS06745	-2	17	2.291022	glycine cleavage system protein GcvH
EN10_RS06750	-1	13	-0.017598	NAD(+) diphosphatase
EN10_RS06755	-1	13	-1.259335	alpha/beta hydrolase
EN10_RS06760	1	12	-3.920419	NUDIX hydrolase family protein
EN10_RS06765	2	14	-4.485422	thioredoxin
EN10_RS06770	3	15	-5.346339	G5 domain-containing protein
EN10_RS06775	2	17	-5.947721	sugar transferase
EN10_RS06780	2	17	-5.580888	hypothetical protein
EN10_RS06785	2	16	-5.296926	DUF2142 domain-containing protein
EN10_RS06790	1	17	-5.768796	glycosyltransferase family 2 protein
EN10_RS06795	2	22	-7.332789	DUF4012 domain-containing protein
EN10_RS06800	1	25	-7.727320	glucose-1-phosphate thymidylyltransferase RfbA
EN10_RS06805	2	29	-8.345834	sugar nucleotide-binding protein
EN10_RS06810	4	33	-8.679315	dTDP-glucose 4,6-dehydratase
EN10_RS06815	5	37	-9.191129	glycosyltransferase
EN10_RS06820	4	32	-8.300103	flippase
EN10_RS06825	3	28	-7.294040	glycosyltransferase
EN10_RS06830	4	30	-7.368769	glycosyltransferase
EN10_RS06835	1	27	-6.995389	hypothetical protein
EN10_RS06840	2	37	-10.595231	glycosyltransferase
EN10_RS06845	3	47	-13.300350	DUF4422 domain-containing protein
EN10_RS06850	4	55	-14.742520	glycosyltransferase
EN10_RS06855	4	49	-13.644638	polysaccharide biosynthesis protein
EN10_RS06860	4	48	-13.517568	nucleotide sugar dehydrogenase
EN10_RS06865	4	42	-11.617299	flippase
EN10_RS06870	2	27	-7.400253	hypothetical protein
EN10_RS06875	0	18	-4.215810	glycosyltransferase
EN10_RS06880	-1	12	-1.411810	sugar transferase
EN10_RS06885	-1	12	-0.077099	hypothetical protein
EN10_RS06890	0	14	-2.463845	DHA2 family efflux MFS transporter permease
EN10_RS06895	2	20	-4.429531	Nramp family divalent metal transporter
EN10_RS06900	4	29	-7.467820	HNH endonuclease family protein
EN10_RS06905	8	41	-11.002825	pyridoxal 5'-phosphate synthase lyase subunit
EN10_RS06910	10	58	-15.154798	pyridoxal 5'-phosphate synthase glutaminase
EN10_RS06915	11	67	-18.635056	NAD-dependent epimerase/dehydratase family
EN10_RS06920	4	42	-10.624100	beta-1,4-galactosyltransferase enhancer
EN10_RS06925	2	34	-8.375075	glycosyl transferase
EN10_RS06930	1	32	-7.055778	glycosyltransferase
EN10_RS06935	0	26	-5.583090	glycosyltransferase family 2 protein
EN10_RS06940	-1	26	-5.419612	EpsG family protein
EN10_RS06945	-2	24	-4.324685	ATP-binding cassette domain-containing protein
EN10_RS06950	0	27	-5.307649	AMP-dependent synthetase/ligase
EN10_RS06955	1	36	-4.223048	Maf family nucleotide pyrophosphatase
EN10_RS06960	1	31	-3.322412	low molecular weight phosphatase family protein
EN10_RS06965	0	23	-2.104636	DUF4012 domain-containing protein
EN10_RS06970	1	15	1.258861	polysaccharide biosynthesis tyrosine autokinase
EN10_RS06975	0	14	3.724553	hypothetical protein
EN10_RS06985	0	14	3.579883	homoserine kinase
EN10_RS06990	0	17	3.043830	homoserine dehydrogenase

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
EN10_RS06755	BACYPHPHTASE	32	0.004	Salmonella/Yersinia modular tyrosine phosphatase si...
>BACYPHPHTASE#Salmonella/Yersinia modular tyrosine phosphatase signature. Length = 468 Score = 31.7 bits (71), Expect = 0.004 Identities = 13/51 (25%), Positives = 19/51 (37%) Query: 210 RMAAARPDLNDEFAKITAPAAVICGEEDPSSAPAVMRPLAERMVNTTVVMT 260 R A R DLN + ++ + C S + R LAE V+ Sbjct: 236 RQTAVRADLNANYIQVGNTRTIACQYPLQSQLESHFRMLAENRTPVLAVLA 286
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
EN10_RS06760	FLGMOTORFLIG	28	0.030	Flagellar motor switch protein FliG signature.
>FLGMOTORFLIG#Flagellar motor switch protein FliG signature. Length = 344 Score = 27.8 bits (62), Expect = 0.030 Identities = 17/80 (21%), Positives = 28/80 (35%), Gaps = 17/80 (21%) Query: 47 IADLRSRMPIAYVEIVP--------VRVDDFGRVTQIGSLLRVANDQSIVERTLIAGRVL 98 I L P EI V +DD +LR + Q + A + + Sbjct: 226 IESLEEEDPELAEEIKKKMFVFEDIVLLDDRSIQR----VLREIDGQEL----AKALKSV 277 Query: 99 YHESLREAIARNISKDMGEL 118 ++E I +N+SK + Sbjct: 278 -DIPVQEKIFKNMSKRAASM 296
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
EN10_RS06810	NUCEPIMERASE	143	2e-42	Nucleotide sugar epimerase signature.
>NUCEPIMERASE#Nucleotide sugar epimerase signature. Length = 334 Score = 143 bits (363), Expect = 2e-42 Identities = 73/339 (21%), Positives = 132/339 (38%), Gaps = 37/339 (10%) Query: 9 NIIVTGGCGFIGSNFVHYVYNNHPDVHITVLDALT--YAGNLE--NIKPILGDRVEFVHG 64 +VTG GFIG + + V +D L Y +L+ ++ + +F Sbjct: 2 KYLVTGAAGFIGFHVSKRLLEAGHQV--VGIDNLNDYYDVSLKQARLELLAQPGFQFHKI 59 Query: 65 NICDAQLLDELVPGH--DAIVHYAAESHNDNSIANPEPFVKTNVEGTFRLLEAARKYDVR 122 ++ D + + +L + + S+ NP + +N+ G +LE R ++ Sbjct: 60 DLADREGMTDLFASGHFERVFISPHRLAVRYSLENPHAYADSNLTGFLNILEGCRHNKIQ 119 Query: 123 FHHI---STDEVYGDLALDDPAKFTEETPY-HPSSPYSSTKASSDMLVRAWHRTYGLRTT 178 H+ S+ VYG L+ F+ + HP S Y++TK +++++ + YGL T Sbjct: 120 --HLLYASSSSVYG---LNRKMPFSTDDSVDHPVSLYAATKKANELMAHTYSHLYGLPAT 174 Query: 179 ISNCSNNYGPYQHVEKFIPRQITNIIEGIRPKLYGDGLNVRDWIHTED------------ 226 YGP+ + + + ++EG +Y G RD+ + +D Sbjct: 175 GLRFFTVYGPWGRPDMALFKFTKAMLEGKSIDVYNYGKMKRDFTYIDDIAEAIIRLQDVI 234 Query: 227 -HSSAVWTILTTGRL-----GETYLIGANGERNNLTVLHDILRVMGQPEDAFDWVKDRPG 280 H+ WT+ T Y IG N L L E + + +PG Sbjct: 235 PHADTQWTVETGTPAASIAPYRVYNIG-NSSPVELMDYIQALEDALGIEAKKNMLPLQPG 293 Query: 281 HDRRYAIDSTKLQTELGWRPTHTDFESGLRQTVQWYIDN 319 + D+ L +G+ P T + G++ V WY D Sbjct: 294 DVLETSADTKALYEVIGFTPE-TTVKDGVKNFVNWYRDF 331
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
EN10_RS06855	PF05704	143	7e-43	Capsular polysaccharide synthesis protein
>PF05704#Capsular polysaccharide synthesis protein Length = 307 Score = 143 bits (362), Expect = 7e-43 Identities = 57/244 (23%), Positives = 109/244 (44%), Gaps = 14/244 (5%) Query: 71 VDEQQCANAPIWVMWWQGVDNQAPDIIQACVRSIRKHANGHPVHVISKQNLHEYASIDES 130 + ++ I++ W QG++ +AP I+Q CV S++K++ V +I N E+ I + Sbjct: 61 IIQEPMRQKYIFICWLQGIE-KAPYIVQQCVASVKKNSGDFKVIIIDGNNYKEWVDIPDF 119 Query: 131 VVQAAECGEIPMAVLSDIIRCTLLYQHGGAWIDATVYLTDDINPEVFTHPIYSIPV--HQ 188 +++ + G++ A SDI+R LL ++GG WIDATVY+ D + + + + Sbjct: 120 LIKRWQEGKMLDAWFSDILRLFLLCKYGGLWIDATVYMFDKVPNYIVESNRFMFQSSFLE 179 Query: 189 KNPTR--NWTSYFIASTQGNPLFDYAGRSLTHLVTRGKGIPEYFMLDVMLSVAYTRHAEF 246 T NW + + +P S+ + + + +Y++ +SV Sbjct: 180 SETTHISNWLIFVKSK--NDPFLVGLKNSMVTYLKKKEKPADYYIFHDFVSVMAVSKEYS 237 Query: 247 TAMIDAIPANNEGRFAL----SEQMDSTEAQPRIPSSTYINKLTYQIDYPTTVNGKETIY 302 + NN L + D++ I S++ + KLTY++DY + T Y Sbjct: 238 KYWKEIPYVNNVNPHMLQYLGNLPYDNSMFN-YIKSTSPVQKLTYKLDYNNL--KRNTYY 294 Query: 303 QRLL 306 + Sbjct: 295 DHIF 298
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
EN10_RS06875	FRAGILYSIN	31	0.006	Fragilysin metallopeptidase (M10C) enterotoxin signat...
>FRAGILYSIN#Fragilysin metallopeptidase (M10C) enterotoxin signature. Length = 405 Score = 30.8 bits (69), Expect = 0.006 Identities = 16/67 (23%), Positives = 29/67 (43%), Gaps = 18/67 (26%) Query: 146 LDAWKAIGGFDEKLFIDLVDTEYDIRAL-MKGYCSYRINTV-----------------VL 187 L + G+D++++ + +D + L M + SY +NT V+ Sbjct: 295 LKSNPKAEGYDDQIYFLIRWGTWDNKILGMSWFNSYNVNTASDFEASGMSTTQLMYPGVM 354 Query: 188 THEIGHI 194 HE+GHI Sbjct: 355 AHELGHI 361
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
EN10_RS06890	TCRTETB	129	1e-34	Tetracycline resistance protein TetB signature.
>TCRTETB#Tetracycline resistance protein TetB signature. Length = 458 Score = 129 bits (326), Expect = 1e-34 Identities = 88/415 (21%), Positives = 174/415 (41%), Gaps = 29/415 (6%) Query: 73 LTFITFVGNFTQLQLSAALPTLVSDFHISVTLGQWMTSIFQLTMGVMVPLTAYLTRRFST 132 L ++F ++ L+ +LP + +DF+ W+ + F LT + + L+ + Sbjct: 19 LCILSFFSVLNEMVLNVSLPDIANDFNKPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGI 78 Query: 133 RQIVITSMVVFTLGSVLAWLSPS-FPLALFGRFLEAVGTGVMWPVLQITVFSIFPLARRG 191 +++++ +++ GSV+ ++ S F L + RF++ G ++ + V P RG Sbjct: 79 KRLLLFGIIINCFGSVIGFVGHSFFSLLIMARFIQGAGAAAFPALVMVVVARYIPKENRG 138 Query: 192 MAMGIVGMAMAVAPAIGPTLGGVQTDLNGWRSIFLTLTIIGCLAIVLTTFGLHNF--GQA 249 A G++G +A+ +GP +GG+ W + L I ++T L + Sbjct: 139 KAFGLIGSIVAMGEGVGPAIGGMIAHYIHWSYLLLIPMI-----TIITVPFLMKLLKKEV 193 Query: 250 DTSAHADFFSVGLSIIGFGGLMFGFTNIQAYPFSAPVVWLPMAIGLAGIIWFVLRQFHTE 309 H D + L +G M T+ Y S +V ++ F++ H Sbjct: 194 RIKGHFDIKGIILMSVGIVFFMLFTTS---YSISFLIVS---------VLSFLIFVKHIR 241 Query: 310 KMFKAGKSSRPALLDLSVLRNRSFSIGTIIASLSFFAFSSLIVLIPLFIQDCRGYS-ATI 368 K + P +D + +N F IG + + F + + ++P ++D S A I Sbjct: 242 ------KVTDP-FVDPGLGKNIPFMIGVLCGGIIFGTVAGFVSMVPYMMKDVHQLSTAEI 294 Query: 369 SGLVMLPGAIAQAISQFFGGRALDRFGARPVALIGTILLTAGTACMSTIGLHTWIWSISF 428 +++ PG ++ I + GG +DR G V IG L+ S + L T W ++ Sbjct: 295 GSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSVSFLTASFL-LETTSWFMTI 353 Query: 429 YQFIRQIGMGFVMMPVTTWSLNCLKPSEVSAGSAVTNTVRQIAGAVGSPVMILIM 483 G+ F ++T + LK E AG ++ N ++ G ++ ++ Sbjct: 354 IIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTSFLSEGTGIAIVGGLL 408
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
EN10_RS06905	PHPHTRNFRASE	29	0.037	Phosphoenolpyruvate-protein phosphotransferase sign...
>PHPHTRNFRASE#Phosphoenolpyruvate-protein phosphotransferase signature. Length = 572 Score = 28.6 bits (64), Expect = 0.037 Identities = 19/114 (16%), Positives = 42/114 (36%), Gaps = 16/114 (14%) Query: 118 REFDVPFVCGAKNLGEALRRIEEGASMIRTKGEPGTGDVIQAVRHMRTMTKQIRELASLR 177 R ++P V G K + E ++ G+ D I+ + + ++++ R Sbjct: 196 RSLEIPAVVGTKEVTEKIQ-----------HGDMVIVDGIEGIVIVNPTEEEVKAYEEKR 244 Query: 178 DDEVYEAAKQLAVPYELAKYVHDNGRLPVVNFAAGGVATPADAALMMELGAEGV 231 + + + E + D + + A + TP D ++ G EG+ Sbjct: 245 AAFEKQKQEWAKLVGEPST-TKDGAHVEL----AANIGTPKDVDGVLANGGEGI 293
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
EN10_RS06915	NUCEPIMERASE	477	e-173	Nucleotide sugar epimerase signature.
>NUCEPIMERASE#Nucleotide sugar epimerase signature. Length = 334 Score = 477 bits (1230), Expect = e-173 Identities = 179/344 (52%), Positives = 234/344 (68%), Gaps = 19/344 (5%) Query: 5 TILITGAAGFIGWNLAERLLHSYPDITIVGLDNLNDYYDVKLKEARLERLTKYPNLIFEK 64 L+TGAAGFIG+++++RLL + + VG+DNLNDYYDV LK+ARLE L P F K Sbjct: 2 KYLVTGAAGFIGFHVSKRLLEAGHQV--VGIDNLNDYYDVSLKQARLELL-AQPGFQFHK 58 Query: 65 GDLSDKKLIGRLFEKHHFDVVVNLGAQAGVRYSITNPDAYVSSNLIGFYNILEACRHNPV 124 DL+D++ + LF HF+ V + VRYS+ NP AY SNL GF NILE CRHN + Sbjct: 59 IDLADREGMTDLFASGHFERVFISPHRLAVRYSLENPHAYADSNLTGFLNILEGCRHNKI 118 Query: 125 EHLVYASSSSVYGGNKKVPFSTEDKVDNPVSLYAATKKSNELMAHAYSKLYDIPSTGLRF 184 +HL+YASSSSVYG N+K+PFST+D VD+PVSLYAATKK+NELMAH YS LY +P+TGLRF Sbjct: 119 QHLLYASSSSVYGLNRKMPFSTDDSVDHPVSLYAATKKANELMAHTYSHLYGLPATGLRF 178 Query: 185 FTVYGPAGRPDMAYFGFTNKLLAGETIKIFNYGNCQRDFTYIDDIVEGIVRVIQGAPTR- 243 FTVYGP GRPDMA F FT +L G++I ++NYG +RDFTYIDDI E I+R+ P Sbjct: 179 FTVYGPWGRPDMALFKFTKAMLEGKSIDVYNYGKMKRDFTYIDDIAEAIIRLQDVIPHAD 238 Query: 244 -----QTGEDGLPVPPYALYNIGCGHPENLLDFVTTLQEELIRAEVLPDDYDFEAHKELV 298 +TG + PY +YNIG P L+D++ L++ L EA K ++ Sbjct: 239 TQWTVETGTPAASIAPYRVYNIGNSSPVELMDYIQALEDAL----------GIEAKKNML 288 Query: 299 PMQPGDVPVTYADTEALTRDYGYRPTTSLRDGLRRFAEWYKQYY 342 P+QPGDV T ADT+AL G+ P T+++DG++ F WY+ +Y Sbjct: 289 PLQPGDVLETSADTKALYEVIGFTPETTVKDGVKNFVNWYRDFY 332
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
EN10_RS06975	IGASERPTASE	52	6e-10	IgA-specific serine endopeptidase (S6) signature.
>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature. Length = 1541 Score = 52.0 bits (124), Expect = 6e-10 Identities = 36/159 (22%), Positives = 66/159 (41%), Gaps = 7/159 (4%) Query: 61 PSAAVAPTEQIDAKENDAELKRREEALNKQ-AEELDKLKRELDEQQKSQSERQTQLDERE 119 P A P+E + +++ + + N+Q A E RE+ ++ KS + TQ +E Sbjct: 1027 PPAPATPSETTETVAENSKQESKTVEKNEQDATETTAQNREVAKEAKSNVKANTQTNEVA 1086 Query: 120 KSIASKEEA-----KKQAEVEAAQRKKAEQEQAAAAEKARAEAEAAERERAQAQ-AAQAA 173 +S + +E K+ A VE ++ K E E+ K ++ + + Q A+ A Sbjct: 1087 QSGSETKETQTTETKETATVEKEEKAKVETEKTQEVPKVTSQVSPKQEQSETVQPQAEPA 1146 Query: 174 AEEAARQQEQAPQVVEQQTSRIVHPGAFCSTAGETGVSQ 212 E + PQ T+ P S+ E V++ Sbjct: 1147 RENDPTVNIKEPQSQTNTTADTEQPAKETSSNVEQPVTE 1185 Score = 48.1 bits (114), Expect = 1e-08 Identities = 26/149 (17%), Positives = 54/149 (36%), Gaps = 7/149 (4%) Query: 52 NASKPAGTVPSAAVAPTEQIDAKENDAELKRREEALNKQAEELDKLKRELDEQQKSQSER 111 N+ + + TV TE A+ + N Q E+ + E E Q ++++ Sbjct: 1043 NSKQESKTVEKNEQDATETTAQNREVAKEAKSNVKANTQTNEVAQSGSETKETQTTETKE 1102 Query: 112 QTQLDEREKSIA----SKEEAKKQAEVEAAQRKKAEQEQAAAAEKARAEAEAAERERAQA 167 +++ EK+ ++E K ++V Q + + A + + ++Q Sbjct: 1103 TATVEKEEKAKVETEKTQEVPKVTSQVSPKQEQSETVQPQAEPARENDPTVNIKEPQSQ- 1161 Query: 168 QAAQAAAEEAARQQEQA--PQVVEQQTSR 194 A E+ A++ V E T Sbjct: 1162 TNTTADTEQPAKETSSNVEQPVTESTTVN 1190

EN10_RS07090

EN10_RS07155

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
EN10_RS07090	0	14	-3.352207	ATP synthase F0 subunit C
EN10_RS07095	0	14	-3.603682	F0F1 ATP synthase subunit A
EN10_RS07100	1	19	-4.152698	homoserine O-succinyltransferase
EN10_RS07105	2	28	-5.334811	MFS transporter
EN10_RS07110	4	37	-7.983904	nucleoside hydrolase
EN10_RS07120	5	45	-10.695756	*hypothetical protein
EN10_RS07125	4	40	-10.587828	hypothetical protein
EN10_RS07130	5	42	-10.417519	hypothetical protein
EN10_RS07135	5	30	-8.741122	hypothetical protein
EN10_RS07140	4	24	-7.566930	Rep family protein
EN10_RS07150	2	18	-6.304315	DUF262 domain-containing protein
EN10_RS07155	1	16	-3.827275	adenine-specific methyltransferase EcoRI family

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
EN10_RS07105	TCRTETB	78	2e-17	Tetracycline resistance protein TetB signature.
>TCRTETB#Tetracycline resistance protein TetB signature. Length = 458 Score = 77.6 bits (191), Expect = 2e-17 Identities = 77/446 (17%), Positives = 170/446 (38%), Gaps = 27/446 (6%) Query: 34 LNASMLSPVLTTMRVELNTTDAEIGLTQTVFFTACAVFSLFLPRLGDLKGRKKVLIGMLA 93 LN +L+ L + + N A T F ++ + +L D G K++L+ + Sbjct: 28 LNEMVLNVSLPDIANDFNKPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGII 87 Query: 94 CTAIGCVISACA-TNVTMLMIGRVIQGVAGPVVPMTLLMLHAEVTDNARYARLMAILTSV 152 G VI + ++L++ R IQG P ++++ A + ++ S+ Sbjct: 88 INCFGSVIGFVGHSFFSLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSI 147 Query: 153 NGGIAGVDALLGGWLAGNFGFRSVFWVMCGVAVLAVVLVWSFARESTADETPKMDWLGAI 212 GV +GG +A + + + + L+ +E D G I Sbjct: 148 VAMGEGVGPAIGGMIAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGH--FDIKGII 205 Query: 213 LLSAAFLAAYLAINEIEKLAGANWWLIAIEIVVAAVLFVIFWHVENAQKNPMVTTTYLKE 272 L+S + L + ++V+ + F+IF +P V K Sbjct: 206 LMSVGIVFFMLFTT-------SYSISF---LIVSVLSFLIFVKHIRKVTDPFVDPGLGKN 255 Query: 273 RRTWGLLLTTLLTMTGVFAVMNGIVPALAQDTSVGASMSADTVSLATLTPYALVGLVFGP 332 ++ L +F + G V + +S + + P + ++FG Sbjct: 256 I---PFMIGVLCGGI-IFGTVAGFVSMVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGY 311 Query: 333 VAGVIASKMGYRSTLRGGLIVSAIAMVFGVFVCQSPHIWGLVVLSVVLGLSYAGTANIML 392 + G++ + G L G+ +++ + F+ ++ + +++ VLG + T ++ Sbjct: 312 IGGILVDRRGPLYVLNIGVTFLSVSFLTASFLLETTSWFMTIIIVFVLG-GLSFTKTVIS 370 Query: 393 NGLGIVLSPEDNPGYLPGLNAGAFNLGAGLSYAILYGV--------QQGFSDAHGATMGY 444 + L ++ + LN +F L G AI+ G+ + + +T Y Sbjct: 371 TIVSSSLKQQEAGAGMSLLNFTSF-LSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLY 429 Query: 445 TGAMIAGIILLVLAFLSSLLIPKPAQ 470 + ++ ++V+++L +L + K +Q Sbjct: 430 SNLLLLFSGIIVISWLVTLNVYKHSQ 455

EN10_RS07875

EN10_RS07900

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
EN10_RS07875	-1	13	3.781469	ROK family protein
EN10_RS07880	-1	13	3.586810	alpha-galactosidase
EN10_RS07885	-1	16	3.320501	nucleoside deaminase
EN10_RS07890	-1	17	3.172703	Na+/H+ antiporter
EN10_RS07895	-1	15	3.441499	alpha/beta hydrolase-fold protein
EN10_RS07900	-1	17	3.103708	hypothetical protein

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
EN10_RS07885	PF00577	31	0.002	Outer membrane usher protein FimD
>PF00577#Outer membrane usher protein FimD Length = 878 Score = 31.0 bits (70), Expect = 0.002 Identities = 9/45 (20%), Positives = 17/45 (37%), Gaps = 1/45 (2%) Query: 12 GRGGIAMTQYAEAMGEALAQARMARSAGDVPVGAVVLDAHGEVIG 56 RG I ++ +G + + +P GA+V + G Sbjct: 781 TRGAIVRAEFKARVGIK-LLMTLTHNNKPLPFGAMVTSESSQSSG 824
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
EN10_RS07900	CHANLCOLICIN	35	6e-04	Channel forming colicin signature.
>CHANLCOLICIN#Channel forming colicin signature. Length = 522 Score = 35.4 bits (81), Expect = 6e-04 Identities = 35/198 (17%), Positives = 67/198 (33%), Gaps = 11/198 (5%) Query: 291 AKTLDTLKSAVADAEKLDQQSVAECAVDANTAAIEKTTDSLRNTANEITKAKSALDAATK 350 A+T LK A A+ ++L S AV+ + + EI S L ++ Sbjct: 165 AETERQLKLAEAEEKRLAALSEEAKAVEIAQKKLSAAQSEVVKMDGEIKTLNSRLSSSIH 224 Query: 351 AVNDSKNMQSQSAARDELTKAIDDAQKILDASANAVTDEQVRTDLENRIADARKLADGQN 410 A M++ + R+EL +A +++ + R+ Sbjct: 225 A--RDAEMKTLAGKRNELAQASAKYKELDELVKKLSPRANDPLQNRPFFEATRRRVGAGK 282 Query: 411 LNAEDVTAMVKTLTDAGTAVQQSQTEYANQQAATAQQAAEEAQRQAEAQAQAAQQAQQNA 470 + E K +T + T + + + Q+A + A +A++N Sbjct: 283 IREE----KQKQVTASETRINRINADITQ-----IQKAISQVSNNRNAGIARVHEAEENL 333 Query: 471 AQAQQQNAQTTTPDTSDA 488 +AQ + D DA Sbjct: 334 KKAQNNLLNSQIKDAVDA 351

EN10_RS00395

EN10_RS00440

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
EN10_RS00395	-2	14	1.690428	cell division protein CrgA
EN10_RS00400	-2	14	1.675510	DUF881 domain-containing protein
EN10_RS00405	-3	13	1.675479	class E sortase
EN10_RS00410	-3	11	1.498001	gamma-glutamyl-gamma-aminobutyrate hydrolase
EN10_RS00415	-4	10	1.523425	Stk1 family PASTA domain-containing Ser/Thr
EN10_RS00420	-3	14	1.286229	serine/threonine protein kinase
EN10_RS00425	-2	14	2.396287	penicillin-binding protein 2
EN10_RS00430	-2	14	2.622467	FtsW/RodA/SpoVE family cell cycle protein
EN10_RS00435	0	14	2.535083	protein phosphatase 2C domain-containing
EN10_RS00440	0	16	2.211739	FHA domain-containing protein

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
EN10_RS00395	IGASERPTASE	29	0.012	IgA-specific serine endopeptidase (S6) signature.
>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature. Length = 1541 Score = 29.3 bits (65), Expect = 0.012 Identities = 24/137 (17%), Positives = 49/137 (35%), Gaps = 9/137 (6%) Query: 3 EEELHKTDADVSANDAADSNATVTDDVVVDA-ATDSEGDAVAVESETVTE-----EPQTD 56 +L+ + + +N T +++ D + S + +A E P Sbjct: 977 RYDLYNPEVEKRNQTVDTTNITTPNNIQADVPSVPSNNEEIARVDEAPVPPPAPATPSET 1036 Query: 57 SEKKAE---REEKRAEDNEKRAEEAAVVSEEEADDLSLDKIQAVLNSTIDEKDMSPQMRR 113 +E AE +E K E NE+ A E + E A + + + + + + + Sbjct: 1037 TETVAENSKQESKTVEKNEQDATETTAQNREVAKEAKSNVKANTQTNEVAQSGSETKETQ 1096 Query: 114 MVKRQQENTKRVEESIK 130 + ++ T EE K Sbjct: 1097 TTETKETATVEKEEKAK 1113 Score = 28.5 bits (63), Expect = 0.018 Identities = 23/126 (18%), Positives = 41/126 (32%), Gaps = 21/126 (16%) Query: 30 VVDAATDSEGDAVAVESETVTEEPQTDSEKKAEREEKRAEDNEKRAEEAAVVSE------ 83 V +E E++ T+ +T E+EEK + EK E V S+ Sbjct: 1076 VKANTQTNEVAQSGSETKE-TQTTETKETATVEKEEKAKVETEKTQEVPKVTSQVSPKQE 1134 Query: 84 -------------EEADDLSLDKIQAVLNSTIDEKDMSPQMRRMVKRQQENTKRVEESIK 130 E +++ + Q+ N+T D + + + V Q + Sbjct: 1135 QSETVQPQAEPARENDPTVNIKEPQSQTNTTADTEQPAKETSSNV-EQPVTESTTVNTGN 1193 Query: 131 GTKANP 136 NP Sbjct: 1194 SVVENP 1199
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
EN10_RS00415	YERSSTKINASE	32	0.011	Yersinia serine/threonine protein kinase signature.
>YERSSTKINASE#Yersinia serine/threonine protein kinase signature. Length = 732 Score = 32.0 bits (72), Expect = 0.011 Identities = 42/170 (24%), Positives = 77/170 (45%), Gaps = 22/170 (12%) Query: 25 AEVHIAL-DTRLGRTVAVKIMRADLAHDEIF--LTRFRREAHSVAQMNNPNIVNIYDSGE 81 E HI++ +T+ + + KI R+ +A +F L ++ + + +PN+ N++ G Sbjct: 145 GESHISIIETKDKQRLVAKIERS-IAEGHLFAELEAYKHIYKTAGK--HPNLANVH--GM 199 Query: 82 EVVTLDNGQTEHLPYLIMEYVQG----QTLRDII------KANGALSVSDTEHVMIGVLN 131 VV N + E L +M+ V G TLR + K N + + +L+ Sbjct: 200 AVVPYGNRKEEAL---LMDEVDGWRCSDTLRTLADSWKQGKINSEAYWGTIKFIAHRLLD 256 Query: 132 ALEYSHRMGIIHRDIKPGNIMISE-QGIVKVMDFGIARALDDSATTMTKS 180 + + G++H DIKPGN++ G V+D G+ + T+S Sbjct: 257 VTNHLAKAGVVHNDIKPGNVVFDRASGEPVVIDLGLHSRSGEQPKGFTES 306
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
EN10_RS00420	YERSSTKINASE	29	0.035	Yersinia serine/threonine protein kinase signature.
>YERSSTKINASE#Yersinia serine/threonine protein kinase signature. Length = 732 Score = 28.9 bits (64), Expect = 0.035 Identities = 43/169 (25%), Positives = 74/169 (43%), Gaps = 24/169 (14%) Query: 132 GVIHRDVKPANIMVSD-NGEVKITDFGVSYSTNQEQITQDGMVVGTAQYISPEQAQGQ-Q 189 GV+H D+KP N++ +GE + D G+ +S + EQ G T + +PE G Sbjct: 265 GVVHNDIKPGNVVFDRASGEPVVIDLGL-HSRSGEQ--PKGF---TESFKAPELGVGNLG 318 Query: 190 ATPQSDIYSL------GVVAYEGLCGHRPFTGATPVDIAAAHV--NNPVP---PLPNTVD 238 A+ +SD++ + + +E +P G + AHV N P P V+ Sbjct: 319 ASEKSDVFLVVSTLLHCIEGFEKNPEIKPNQGLRFITSEPAHVMDENGYPIHRPGIAGVE 378 Query: 239 FQLSQFVMSMLAKDPADRPQDALTVARVLSRIERRLLDQQTA---LSDT 284 ++F+ +L RP AR+ + +D+++A L DT Sbjct: 379 TAYTRFITDILGVSADSRPDS--NEARLHEFLSDGTIDEESAKQILKDT 425
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
EN10_RS00425	BLACTAMASEA	29	0.030	Beta-lactamase class A signature.
>BLACTAMASEA#Beta-lactamase class A signature. Length = 286 Score = 29.4 bits (66), Expect = 0.030 Identities = 24/108 (22%), Positives = 44/108 (40%), Gaps = 27/108 (25%) Query: 213 ELYPPGSTFKTVVAAAAL---DSGKYQLDTQIPAGASYTL---PGTQTQLTNAEEPGNGT 266 E +P STFK V+ A L D+G QL+ +I + P ++ L Sbjct: 60 ERFPMMSTFKVVLCGAVLARVDAGDEQLERKIHYRQQDLVDYSPVSEKHL---------- 109 Query: 267 DGKISLKD----AMAWSSNTA----FAQLGVALGDESVSNMAKKFGFD 306 +++ + A+ S N+A A +G G ++ ++ G + Sbjct: 110 ADGMTVGELCAAAITMSDNSAANLLLATVG---GPAGLTAFLRQIGDN 154
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
EN10_RS00440	SSBTLNINHBTR	27	0.031	Streptomyces subtilisin inhibitor signature.
>SSBTLNINHBTR#Streptomyces subtilisin inhibitor signature. Length = 144 Score = 27.1 bits (59), Expect = 0.031 Identities = 20/62 (32%), Positives = 29/62 (46%), Gaps = 1/62 (1%) Query: 57 ALAVSASAQPLP-VSAPTPSAAAPSDTAPTPTLLVIIDGPLAGTSVPLNSSVITLGRAAS 115 L +A PL S +P+ A S AP+ +L + G A T+ PL + +T AS Sbjct: 12 GLTATAVCGPLAGASLASPATAPASLYAPSALVLTVGHGESAATAAPLRAVTLTCAPTAS 71 Query: 116 NT 117 T Sbjct: 72 GT 73

EN10_RS00875

EN10_RS00940

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
EN10_RS00875	-1	17	2.353078	TetR/AcrR family transcriptional regulator
EN10_RS00880	-1	17	2.238003	YhgE/Pip domain-containing protein
EN10_RS00885	-2	15	2.560808	YhgE/Pip domain-containing protein
EN10_RS00890	0	13	2.958541	MptD family putative ECF transporter S
EN10_RS00895	0	14	3.355012	energy-coupling factor transporter transmembrane
EN10_RS00900	1	16	2.983596	ATP-binding cassette domain-containing protein
EN10_RS00905	1	18	2.348873	ZinT/AdcA family metal-binding protein
EN10_RS00910	1	21	3.248438	patatin family protein
EN10_RS00915	-1	17	3.132599	hypothetical protein
EN10_RS00920	-1	17	3.249912	hypothetical protein
EN10_RS00930	0	15	3.467924	*C69 family dipeptidase
EN10_RS00935	1	13	3.700480	TetR/AcrR family transcriptional regulator
EN10_RS00940	1	16	3.769139	signal recognition particle-docking protein

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
EN10_RS00875	HTHTETR	57	2e-12	TetR bacterial regulatory protein HTH signature.
>HTHTETR#TetR bacterial regulatory protein HTH signature. Length = 215 Score = 57.3 bits (138), Expect = 2e-12 Identities = 25/180 (13%), Positives = 57/180 (31%), Gaps = 18/180 (10%) Query: 38 RRERTDGKITRAVLELILAKGIGAVSIEAVAKRSGVAKTTIYRRYANTDDLL-------- 89 + T I L L +G+ + S+ +AK +GV + IY + + DL Sbjct: 8 EAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSEIWELSE 67 Query: 90 RHLSVAVGEPLDFSGFDTTFDGLRGVLKCIVDCFDEELGLKAIGVVLSSSNDY------L 143 ++ E LR +L +++ E + + ++ ++ + Sbjct: 68 SNIGELELEYQA-KFPGDPLSVLREILIHVLESTVTEERRRLLMEIIFHKCEFVGEMAVV 126 Query: 144 ANLAERVVTPAQQRFADFIGRGVSSGAFRGDVSVPFLFQTV---IGSMMAAKALSDSSHD 200 + + R + + + D+ + I +M + S D Sbjct: 127 QQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMTRRAAIIMRGYISGLMENWLFAPQSFD 186
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
EN10_RS00880	FLAGELLIN	33	0.006	Flagellin signature.
>FLAGELLIN#Flagellin signature. Length = 507 Score = 32.7 bits (74), Expect = 0.006 Identities = 32/300 (10%), Positives = 67/300 (22%), Gaps = 30/300 (10%) Query: 92 TFTKSSAEAIDGTKSGKYYAAVVIPKNFSKNMMTFFTDDAYHATLDYYNNEKLNALAPKV 151 + + + + + + T D NN ++ Sbjct: 199 AVGANKYRVDVNSGAVVTDTTA----PTVPDKVYVNAANGQLTTDDAENNTAVDLFKTTK 254 Query: 152 TGQGADTIAATINQTFSKTLTDTALNLASSMLDMLDKPEYQQRLKSFNGNIADLAGMLND 211 + G A DT + G Sbjct: 255 STAGTAEAKAIAGAIKGGKEGDTFDYKGVTFT---------------IDTKTGNDGNGKV 299 Query: 212 SAAVLRTYSSLTDSAKTLLASSNKLIADARASAKTGVDDLSAAKTGVSDLSGALGTSVNA 271 S + +LT + T A++ + + + S Sbjct: 300 STTINGEKVTLTVADITAGAANVDAATLQSSKNVYTSVVNGQFTFDDKTKNESAKLSDLE 359 Query: 272 LGTAIDTSKTSLGSLNDRVDELFSAVDKQAGDTSAAINNLAGNVRTEATAYATTRGRLQE 331 A+ + + AG T +G A + Sbjct: 360 ANNAVKGESKITVNGAEYTANAAGDKVTLAGKTMFIDKTASGVSTLINEDAAAAKKSTAN 419 Query: 332 LLDKSDNLGPVAKSAVQAFINRIGAVADDLNQTADRLTTAANDVDSKVGSSKETRQEIKD 391 L D+ ++++ AV L +R +A ++ + V + R I+D Sbjct: 420 PLASIDSA-----------LSKVDAVRSSLGAIQNRFDSAITNLGNTVTNLNSARSRIED 468
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
EN10_RS00885	ABC2TRNSPORT	34	0.003	ABC-2 type transport system membrane protein signat...
>ABC2TRNSPORT#ABC-2 type transport system membrane protein signature. Length = 262 Score = 33.8 bits (77), Expect = 0.003 Identities = 23/98 (23%), Positives = 45/98 (45%), Gaps = 3/98 (3%) Query: 604 LTSLAYLAIAYALSATFMHVGKALVVALVMLQIPGASGLYPIEMMPDFYRALYPFFPFTY 663 SL + A A S + + LV+ +L + GA ++P++ +P ++ F P ++ Sbjct: 161 FASLGMVVTALAPSYDYFIFYQTLVIT-PILFLSGA--VFPVDQLPIVFQTAARFLPLSH 217 Query: 664 SINAFRETIAGFYDGDWLTKMLMLALFTAIAFFVGLVV 701 SI+ R + G D + L ++ I FF+ + Sbjct: 218 SIDLIRPIMLGHPVVDVCQHVGALCIYIVIPFFLSTAL 255
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
EN10_RS00905	adhesinb	102	3e-26	Adhesin B signature.
>adhesinb#Adhesin B signature. Length = 310 Score = 102 bits (255), Expect = 3e-26 Identities = 51/307 (16%), Positives = 111/307 (36%), Gaps = 49/307 (15%) Query: 29 LAGLAVCGLAASTLSGCGNNAGENRTSAAAARQTTDSCDTTINVVASVNQWGSLAQQLGG 88 L + LA L+ C + +T++ + +NVVA+ + + + + G Sbjct: 4 CRFLVLLLLAFVGLAACSSQKS-----------STETGSSKLNVVATNSIIADITKNIAG 52 Query: 89 SCVNVTSIINSTAADPHDHEATPADLTKLARADVVVLNG----AGYDGWAQKAQLDEGRQ 144 +N+ SI+ DPH++E P D+ K ++AD++ NG G + W K Sbjct: 53 DKINLHSIVPVGQ-DPHEYEPLPEDVKKTSQADLIFYNGINLETGGNAWFTKL---VENA 108 Query: 145 RIVKASSLMGIADSQDDHDHEEGEGHHHHHGTVNPHLWFSPAAVLKMSEAITSAYVTKSG 204 + + +++ D E G +PH W + + ++ I K Sbjct: 109 KKKENKDYYAVSEGVDVIYLEGQS----EKGKEDPHAWLNLENGIIYAQNIAKRLSEKDP 164 Query: 205 EASETAATARRHSNTWNAEYAEYTALVN------RARAKNL---QRRYVATESIIGHLLD 255 + T+ Y ++ + + N+ ++ V +E + Sbjct: 165 A----------NKETYEKNLKAYVEKLSALDKEAKEKFNNIPGEKKMIVTSEGCFKYFSK 214 Query: 256 YIGATDKTPDSYTNAMNNDAEPSASDLKNALDTVGSSNVDMLIVNPQEMGGFAKKLDAAA 315 P +Y +N + E + +K ++ + + V L V E + + + Sbjct: 215 AYN----VPSAYIWEINTEEEGTPDQIKTLVEKLRKTKVPSLFV---ESSVDDRPMKTVS 267 Query: 316 RESGKTI 322 +++ I Sbjct: 268 KDTNIPI 274
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
EN10_RS00915	HTHTETR	27	0.046	TetR bacterial regulatory protein HTH signature.
>HTHTETR#TetR bacterial regulatory protein HTH signature. Length = 215 Score = 27.3 bits (60), Expect = 0.046 Identities = 30/227 (13%), Positives = 63/227 (27%), Gaps = 39/227 (17%) Query: 19 TPTRQSIERALVEWMTQTGRPASDLSVSALCKKAFVARSTFYANYRHMGQVYESVENRLL 78 TRQ I + +Q G S S+ + K A V R Y +++ ++ + Sbjct: 10 QETRQHILDVALRLFSQQG--VSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSEIWELSE 67 Query: 79 RQLFECAVPMRVRADGTDETMRSFNAFVKVLHAHENEFRLVLVRQPSSRFVALWKQSLEY 138 + E + + + G + ++L + + + + E+ Sbjct: 68 SNIGELELEYQAKFPGDPLS-----VLREILIHV---LESTVTEERRRLLMEIIFHKCEF 119 Query: 139 H-VWHRLFETERGGAAAVDAVAEWGDGAAPGTSNGGSATSQEGLPAGTDADATNRNELPV 197 + + +R E + LPA Sbjct: 120 VGEMAVVQQAQRNLCLESYDRIEQTLKH---------CIEAKMLPADLMTRRA------- 163 Query: 198 RQGPATVNRALVFDMAATAFIAGVTFWLRTPQAVSLPEIRASVVKLV 244 A + R + + WL PQ+ L + V ++ Sbjct: 164 ----AIIMRGYISGLMEN--------WLFAPQSFDLKKEARDYVAIL 198
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
EN10_RS00935	HTHTETR	71	4e-17	TetR bacterial regulatory protein HTH signature.
>HTHTETR#TetR bacterial regulatory protein HTH signature. Length = 215 Score = 71.2 bits (174), Expect = 4e-17 Identities = 20/105 (19%), Positives = 46/105 (43%) Query: 1 MANASRMSSYQRREQLLRIGRSLFAAKGFEAVSVEEIAATAKVSKPIVYEHFGGKEGLYA 60 MA ++ + + R+ +L + LF+ +G + S+ EIA A V++ +Y HF K L++ Sbjct: 1 MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFS 60 Query: 61 VVVDREMRALNDTLVAALSAQDVHPRQIVERTALALLTYIEENAE 105 + + + + + + P ++ + +L Sbjct: 61 EIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVTEER 105
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
EN10_RS00940	IGASERPTASE	52	4e-09	IgA-specific serine endopeptidase (S6) signature.
>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature. Length = 1541 Score = 52.0 bits (124), Expect = 4e-09 Identities = 34/215 (15%), Positives = 68/215 (31%), Gaps = 20/215 (9%) Query: 29 SRRNAINKSTEHAKAAADARLAAERAQAEQEEREAHEQAKEPAESGARQSSGGKPAEPTN 88 S++ + A E A+ + +A+ Q E A+SG+ ++ + E Sbjct: 1044 SKQESKTVEKNEQDATETTAQNREVAKEAKSNVKANTQTNEVAQSGS-ETKETQTTETKE 1102 Query: 89 EEKIEAEEPAISRAELEAQERERLSQTPPKLAAAEEVPGEESAQAESVVEDLAVVDEVPT 148 +E EE A E + + SQ PK +E V + E PT Sbjct: 1103 TATVEKEEKAKVETEKTQEVPKVTSQVSPKQEQSETVQPQAEPARE----------NDPT 1152 Query: 149 PPAEQIEKPAGKKSRLQRLRERLSKSSNPFGKALFNILAKDQLSEA--DWEDVEDTLLLA 206 ++ + + ++ + S + + + + + A Sbjct: 1153 VNIKEPQSQTNTTADTEQPAKETSSNVEQ-------PVTESTTVNTGNSVVENPENTTPA 1205 Query: 207 DVGAEASEELVDKLRTDARVTGESDPRQLRDALRS 241 + E +K + R + S P + A S Sbjct: 1206 TTQPTVNSESSNKPKNRHRRSVRSVPHNVEPATTS 1240 Score = 30.4 bits (68), Expect = 0.021 Identities = 35/238 (14%), Positives = 65/238 (27%), Gaps = 13/238 (5%) Query: 35 NKSTEHAKAAADARLAAERAQAEQEEREAHEQAKEPAESGARQSSGGKPAEPTNEEKIEA 94 N E D + + +E A PA P+ + A Sbjct: 982 NPEVEKRNQTVDTTNITTPNNIQADVPSVPSNNEEIARVDEAPVPPPAPATPSETTETVA 1041 Query: 95 EEPAISRAELEAQERERLSQTPPKLAAAEEVPGEESA--QAESVVEDLAVVDEVPTPPAE 152 E +E E++ T A+E A Q V + + E T + Sbjct: 1042 ENSKQESKTVEKNEQDATETTAQNREVAKEAKSNVKANTQTNEVAQSGSETKETQTTETK 1101 Query: 153 Q---IEKPAGKKSRLQRLRERLSKSSNPFGKALFNILAKDQLSEADWEDVEDTLLLADVG 209 + +EK K ++ +E +S K ++ +A+ D + Sbjct: 1102 ETATVEKEEKAKVETEKTQEVPKVTSQVSPK---QEQSETVQPQAEPARENDPTVNIK-- 1156 Query: 210 AEASEELVDKLRTDARVTGESDPRQLRDALRSELLALVGPDMDRTLEAEKPDAKKPSV 267 E + D + + + G + E P +P+V Sbjct: 1157 -EPQSQ--TNTTADTEQPAKETSSNVEQPVTESTTVNTGNSVVENPENTTPATTQPTV 1211

EN10_RS01040

EN10_RS01085

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
EN10_RS01040	0	14	4.576725	pilus assembly protein
EN10_RS01045	-2	15	4.273130	hypothetical protein
EN10_RS01050	-2	15	3.851252	DNA polymerase III subunit gamma/tau
EN10_RS01055	0	19	2.984841	recombination mediator RecR
EN10_RS01060	0	21	2.649421	aspartate kinase
EN10_RS01065	0	22	2.389758	ACT domain-containing protein
EN10_RS01070	0	20	2.769553	aspartate-semialdehyde dehydrogenase
EN10_RS01075	1	20	3.278578	DUF5701 family protein
EN10_RS01080	2	21	3.481329	2-isopropylmalate synthase
EN10_RS01085	2	20	4.205551	penicillin-binding protein

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
EN10_RS01040	PilS_PF08805	28	0.013	PilS N terminal
>PilS_PF08805#PilS N terminal Length = 185 Score = 27.6 bits (61), Expect = 0.013 Identities = 18/65 (27%), Positives = 26/65 (40%), Gaps = 2/65 (3%) Query: 10 LRRLQCDDEGAATAEFAVVLPVIVML--AALMLYLGRASVVSVGCQDAAANAARALMVQG 67 RR + D+GA E +V+ VIV+L +A LY S + + A M Sbjct: 18 ARRKKEQDKGATLMEVLLVVGVIVVLAASAYKLYSMVQSNIQSSNEQNNVLTVIANMKSL 77 Query: 68 SGSGS 72 G Sbjct: 78 KFQGR 82
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
EN10_RS01050	IGASERPTASE	32	0.010	IgA-specific serine endopeptidase (S6) signature.
>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature. Length = 1541 Score = 32.3 bits (73), Expect = 0.010 Identities = 38/221 (17%), Positives = 68/221 (30%), Gaps = 27/221 (12%) Query: 567 APPLSKLPPEEAQRIKAKLLQQSLQGIVAHPRESSDSAQQATTSDSSNDAEAHQAAGTTP 626 PP P E + + Q+S + + Q AT + + N A +A Sbjct: 1026 PPPAPATPSETTETVAENSKQES--------KTVEKNEQDATETTAQNREVAKEAKSNVK 1077 Query: 627 TPTESFQQANGNGPQTLPDDDPWAQPVKPVGLRPQALDPEEQAHEEPNRQQPAPEHQHKH 686 T Q N + K ++ EE+A E + Q P+ + Sbjct: 1078 ANT----QTNEVAQSGSETKETQTTETK----ETATVEKEEKAKVETEKTQEVPKVTSQV 1129 Query: 687 VAVPDVSDGIDPWSMPVDEHPHIDFGKVQSIEQQHERLTPAPVHGDDPWSAMPNGPQGFG 746 + S+ + P + P E+ K + T P N Q Sbjct: 1130 SPKQEQSETVQPQAEPARENDPTVNIKEPQSQTNTTADTEQPAK-----ETSSNVEQ--- 1181 Query: 747 TASPVTGGQAMSVEDAYGMSPQGMHAADAGSTGVAGGESQP 787 PVT ++ ++ +P+ A T + ++P Sbjct: 1182 ---PVTESTTVNTGNSVVENPENTTPATTQPTVNSESSNKP 1219
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
EN10_RS01060	CARBMTKINASE	28	0.027	Bacterial carbamate kinase signature.
>CARBMTKINASE#Bacterial carbamate kinase signature. Length = 314 Score = 28.3 bits (63), Expect = 0.027 Identities = 22/124 (17%), Positives = 47/124 (37%), Gaps = 22/124 (17%) Query: 113 IRAVNPDRVRRALNKGSVAIVAG-------FQGQSERGDATTLGRGGSDTSAVALAVALG 165 V + +++ + +G + I +G + +G + + D + LA + Sbjct: 171 KGHVEAETIKKLVERGVIVIASGGGGVPVILEDGEIKGVEAVIDK---DLAGEKLAEEVN 227 Query: 166 ADVCEIYTDVDGVFTADPRIVPTARRIPAIGYDEILEM--------SSCGSKVLALRCVE 217 AD+ I TDV+G + + + +E+ + S G KVLA + Sbjct: 228 ADIFMILTDVNGAALYYGT--EKEQWLREVKVEELRKYYEEGHFKAGSMGPKVLA--AIR 283 Query: 218 YAQR 221 + + Sbjct: 284 FIEW 287
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
EN10_RS01065	OMPADOMAIN	28	0.030	OMPA domain signature.
>OMPADOMAIN#OMPA domain signature. Length = 346 Score = 27.6 bits (61), Expect = 0.030 Identities = 36/171 (21%), Positives = 62/171 (36%), Gaps = 9/171 (5%) Query: 23 ISGVAHDNSESLVTVRGVTDE--PGMAGRVFTR-LAELGVNVDMIVQAGASTGTADISFT 79 + G HD S V GV P +A R+ + +G + + + +S+ Sbjct: 131 VYGKNHDTGVSPVFAGGVEYAITPEIATRLEYQWTNNIGDAHTIGTRPDNGMLSLGVSYR 190 Query: 80 IPGSDAARVETALTAAQDTLGYKSFDVNPNV----GKVAVVGVGMKTHSGLAARFFQALS 135 +AA V A + K F + +V K + G L ++ Sbjct: 191 FGQGEAAPVVAPAPAPAPEVQTKHFTLKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDP 250 Query: 136 EEGINVLMISTSEIRIAAL-VPLSQLN-DAVRALHSAYGLDADKVEAVVYG 184 ++G V++ T I A LS+ +V + G+ ADK+ A G Sbjct: 251 KDGSVVVLGYTDRIGSDAYNQGLSERRAQSVVDYLISKGIPADKISARGMG 301
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
EN10_RS01085	cloacin	48	9e-08	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 48.2 bits (114), Expect = 9e-08 Identities = 28/76 (36%), Positives = 35/76 (46%), Gaps = 5/76 (6%) Query: 730 GSNSGDSGNSGNQNGNTSG---NNGGNNGSNNGSNNGSNNSGSNSG--DSGNSGNQNGNT 784 G N+G SGN NG +G G ++GS S N GS SG G SG+ NG Sbjct: 8 GHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGG 67 Query: 785 GGNNGSQNNTGNGEQT 800 GN+G + TG Sbjct: 68 NGNSGGGSGTGGNLSA 83 Score = 44.3 bits (104), Expect = 2e-06 Identities = 24/84 (28%), Positives = 35/84 (41%), Gaps = 3/84 (3%) Query: 708 GNDNTNGNGGG-ETGGNPSDNNGGSNSGDSGNSGNQNGNTSGNNGGNNGSNNGSNNGSNN 766 G D N G T GN + G G + G+ + + GG GS +G + G + Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGG--GSGSGIHWGGGS 60 Query: 767 SGSNSGDSGNSGNQNGNTGGNNGS 790 N G +GNSG +G G + Sbjct: 61 GHGNGGGNGNSGGGSGTGGNLSAV 84

EN10_RS01670

EN10_RS01705

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
EN10_RS01670	-2	17	2.591416	hypothetical protein
EN10_RS01675	-2	13	2.355495	C1 family peptidase
EN10_RS01680	-2	11	2.306730	3-deoxy-7-phosphoheptulonate synthase
EN10_RS01685	-2	10	2.483611	acetyltransferase
EN10_RS01690	-1	15	2.599063	3-deoxy-7-phosphoheptulonate synthase
EN10_RS01695	1	16	1.878554	5'-methylthioadenosine/S-adenosylhomocysteine
EN10_RS01700	1	17	1.670924	ATP-binding protein
EN10_RS01705	0	18	1.121112	response regulator transcription factor

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
EN10_RS01670	CHANLCOLICIN	47	1e-07	Channel forming colicin signature.
>CHANLCOLICIN#Channel forming colicin signature. Length = 522 Score = 47.4 bits (112), Expect = 1e-07 Identities = 59/320 (18%), Positives = 117/320 (36%), Gaps = 19/320 (5%) Query: 62 SFASLGANAQQMLASAEQTSTELLERAKKDAASARTEAQSQSQTLVNNAKLDAQHLVDDA 121 S A++ A A+ A ++T E RAK AA A+ +A++ L K +V++A Sbjct: 47 SSAAIHATAKWSTAQLKKTQAEQAARAKA-AAEAQAKAKANRDALTQRLK----DIVNEA 101 Query: 122 QAKADTILKNAQSEANTIVTNARQEAEQLRASTAKTVSDQRQALDLELTNSREEHKKRLA 181 + +A A+ + E E+LR + A+ + + + E+ +K + Sbjct: 102 LRHNASRTPSATELAHANNAAMQAEDERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIE 161 Query: 182 SERAQQERELADLSAQTAKTISEQRKSANEEMARLKSETNDQIETALADANKKLADVREQ 241 E+A+ ER+L E+R +A E A+ ++ A ++ K +++ Sbjct: 162 REKAETERQL------KLAEAEEKRLAALSEEAKAVEIAQKKLSAAQSEVVKMDGEIKTL 215 Query: 242 VSKLMTEAQRKAGEITDAAKAHAQEITDAAEVNRTQTMSQVNAEVEQIRADIAAQQD-EA 300 S+L + + E+ A + A + V + + + EA Sbjct: 216 NSRLSSSIHARDAEMKTLAGKRNEL--AQASAKYKELDELVKKLSPRANDPLQNRPFFEA 273 Query: 301 TKKVQELLQSLEESRKS--AKEEAEKEIGQAKQAREEADAYAAEKRESADTEAREIVRKA 358 T++ + EE +K A E I ++A + + R + E Sbjct: 274 TRRRVGAGKIREEKQKQVTASETRINRINADITQIQKAISQVSNNRNAGIARVHE---AE 330 Query: 359 GEEASKQIEDRRKAAQSELD 378 Q + +D Sbjct: 331 ENLKKAQNNLLNSQIKDAVD 350 Score = 34.7 bits (79), Expect = 0.001 Identities = 45/250 (18%), Positives = 90/250 (36%), Gaps = 25/250 (10%) Query: 15 ERVDEAMLAAQNSIDRLREQVKAGDDTVLQLQAQLQEEKNRKANTDNSFASLGANAQQML 74 E + + AAQ+ + ++ ++K + + E A N L Sbjct: 192 EIAQKKLSAAQSEVVKMDGEIKTLNSRLSSSIHARDAEMKTLAGKRNE-----------L 240 Query: 75 ASAEQTSTELLERAKKDAASARTEAQSQSQTLVNNAKLDAQHLVDDAQAKADTILKNAQS 134 A A EL E KK + A Q++ ++ A + ++ Q + + +++ Sbjct: 241 AQASAKYKELDELVKKLSPRANDPLQNRPFFEATRRRVGAGKIREEKQKQ----VTASET 296 Query: 135 EANTIVTNARQEAEQLRASTAKTVSDQRQALDLELTNSREEHKKRLASERAQQERELADL 194 N I + Q + + VS+ R A + + E KK + Q ++ D Sbjct: 297 RINRINADITQIQKAI-----SQVSNNRNAGIARVHEAEENLKKAQNNLLNSQIKDAVDA 351 Query: 195 SAQTAKTISEQRKSANEEMAR-----LKSETNDQIETALADANKKLADVREQVSKLMTEA 249 + +T++E+ +MA+ K + + ALA K + ++ SK +A Sbjct: 352 TVSFYQTLTEKYGEKYSKMAQELADKSKGKKIGNVNEALAAFEKYKDVLNKKFSKADRDA 411 Query: 250 QRKAGEITDA 259 A Sbjct: 412 IFNALASVKY 421
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
EN10_RS01685	IGASERPTASE	32	0.012	IgA-specific serine endopeptidase (S6) signature.
>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature. Length = 1541 Score = 32.0 bits (72), Expect = 0.012 Identities = 15/63 (23%), Positives = 31/63 (49%), Gaps = 2/63 (3%) Query: 465 ALPLDWRAIAHARS-VKARPELALTAEQARERLAQQQKRESETSKENGKEHKDSSGKPNA 523 ++P + IA P A +E E +A+ K+ES+T ++N ++ +++ + Sbjct: 1009 SVPSNNEEIARVDEAPVPPPAPATPSETT-ETVAENSKQESKTVEKNEQDATETTAQNRE 1067 Query: 524 QAK 526 AK Sbjct: 1068 VAK 1070
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
EN10_RS01700	PF06580	34	8e-04	Sensor histidine kinase
>PF06580#Sensor histidine kinase Length = 349 Score = 34.1 bits (78), Expect = 8e-04 Identities = 16/105 (15%), Positives = 37/105 (35%), Gaps = 26/105 (24%) Query: 281 LVENAINY----SPEHAEVTVEVSRGEDQARIRVVDHGIGIPRQLQGRIFERFYRVDPAR 336 LVEN I + P+ ++ ++ ++ + V + G + + Sbjct: 263 LVENGIKHGIAQLPQGGKILLKGTKDNGTVTLEVENTGSLALKNTK-------------- 308 Query: 337 SRLTGGTGLGLSIASHHILA---NQGKLEVWSKPGEGSTFTVMLP 378 TG GL + + ++++ K G+ V++P Sbjct: 309 ----ESTGTGLQNVRERLQMLYGTEAQIKLSEKQGKV-NAMVLIP 348
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
EN10_RS01705	HTHFIS	91	2e-23	FIS bacterial regulatory protein HTH signature.
>HTHFIS#FIS bacterial regulatory protein HTH signature. Length = 484 Score = 91.4 bits (227), Expect = 2e-23 Identities = 33/137 (24%), Positives = 61/137 (44%), Gaps = 3/137 (2%) Query: 2 TRILVVEDEPSYLDPLLYQLTREGYEAHGAATGEEAWELYEEMEFDLVLLDLMLPGISGT 61 ILV +D+ + L L+R GY+ + W + DLV+ D+++P + Sbjct: 4 ATILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAF 63 Query: 62 TLCRRMHELR-RVPIIMVTAKSTENDVIVGLEIGADDYVTKPYSFRELLARIHAVL--RR 118 L R+ + R +P+++++A++T I E GA DY+ KP+ EL+ I L + Sbjct: 64 DLLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAEPK 123 Query: 119 SLEPRASGTPGGNDETL 135 + + Sbjct: 124 RRPSKLEDDSQDGMPLV 140

EN10_RS02880

EN10_RS02905

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
EN10_RS02880	-1	13	0.215511	GNAT family N-acetyltransferase
EN10_RS02885	-2	11	1.201883	DUF1295 domain-containing protein
EN10_RS02890	-2	12	1.024177	iron-containing alcohol dehydrogenase family
EN10_RS02895	-3	13	1.189597	S-ribosylhomocysteine lyase
EN10_RS02900	-2	12	1.360954	N-acetyltransferase
EN10_RS02905	-2	11	1.502557	alanine racemase

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
EN10_RS02880	SACTRNSFRASE	42	1e-07	Streptothricin acetyltransferase signature.
>SACTRNSFRASE#Streptothricin acetyltransferase signature. Length = 173 Score = 42.2 bits (99), Expect = 1e-07 Identities = 16/61 (26%), Positives = 27/61 (44%) Query: 90 RTLYIDDICVDERARGKHVGTELYDHVLDYARAHGYHNVTLNAWAANPAAVKFYESLGMS 149 I+DI V + R K VGT L +++A+ + + + L N +A FY Sbjct: 88 GYALIEDIAVAKDYRKKGVGTALLHKAIEWAKENHFCGLMLETQDINISACHFYAKHHFI 147 Query: 150 V 150 + Sbjct: 148 I 148
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
EN10_RS02890	BACTRLTOXIN	33	0.002	Bacterial toxin signature.
>BACTRLTOXIN#Bacterial toxin signature. Length = 266 Score = 32.6 bits (74), Expect = 0.002 Identities = 18/55 (32%), Positives = 24/55 (43%), Gaps = 1/55 (1%) Query: 12 YDHPVIITGVKSADAFLDYTGEDEFFAPVLR-YDGSSSERNARELAEQAKALDAD 65 DH V T VKS D FL + L+ YD +E +LA++ K D Sbjct: 56 DDHYVSATKVKSVDKFLAHDLIYNISDKKLKNYDKVKTELLNEDLAKKYKDEVVD 110
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
EN10_RS02895	LUXSPROTEIN	156	2e-51	Bacterial autoinducer-2 (AI-2) production protein Lu...
>LUXSPROTEIN#Bacterial autoinducer-2 (AI-2) production protein LuxS signature. Length = 171 Score = 156 bits (395), Expect = 2e-51 Identities = 57/151 (37%), Positives = 84/151 (55%), Gaps = 4/151 (2%) Query: 11 PVVESFQLDHTKVKAPYVRYIDTETGPNGDVISNYDLRLVQPNENAIPTGGLHTIEHTIA 70 P+++SF +DHT++ AP VR T P GD I+ +DLR PN++ + G+HT+EH A Sbjct: 1 PLLDSFTVDHTRMNAPAVRVAKTMQTPKGDTITVFDLRFTAPNKDILSEKGIHTLEHLYA 60 Query: 71 VLLRERIPGY----IDCSPFGCRTGFHLLTWGEHSTEDVAKALKESLEFIANEATWDDVP 126 +R + G ID SP GCRTGF++ G S + VA A ++E + + +P Sbjct: 61 GFMRNHLNGDSVEIIDISPMGCRTGFYMSLIGTPSEQQVADAWIAAMEDVLKVENQNKIP 120 Query: 127 ATTIESCGNYRDHSLFTAKEWAKDILAQGIS 157 CG HSL AK+ AK+IL G++ Sbjct: 121 ELNEYQCGTAAMHSLDEAKQIAKNILEVGVA 151
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
EN10_RS02900	SACTRNSFRASE	30	0.003	Streptothricin acetyltransferase signature.
>SACTRNSFRASE#Streptothricin acetyltransferase signature. Length = 173 Score = 29.9 bits (67), Expect = 0.003 Identities = 16/76 (21%), Positives = 24/76 (31%), Gaps = 10/76 (13%) Query: 86 YAKIEGAWLDNDSYVTIHRIA------SSGLVRHAARDCIEWCL-KHYGNVRVDTHPNNK 138 KI W + Y I IA G+ IEW H+ + ++T N Sbjct: 79 RIKIRSNW---NGYALIEDIAVAKDYRKKGVGTALLHKAIEWAKENHFCGLMLETQDINI 135 Query: 139 AMQHVLESCGFARCGL 154 + H F + Sbjct: 136 SACHFYAKHHFIIGAV 151
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
EN10_RS02905	ALARACEMASE	285	2e-95	Alanine racemase signature.
>ALARACEMASE#Alanine racemase signature. Length = 356 Score = 285 bits (732), Expect = 2e-95 Identities = 117/405 (28%), Positives = 163/405 (40%), Gaps = 54/405 (13%) Query: 31 PAQVIVDLAALRDNMRHLVDITNREGGP-TEVMGVVKADAYGHGLVPSALAALAGGATWL 89 P Q +DL AL+ N+ + R+ V VVKA+AYGHG+ A A Sbjct: 4 PIQASLDLQALKQNLSIV-----RQAATHARVWSVVKANAYGHGIERIWSAIGA--TDGF 56 Query: 90 GVAQAREALALRNAGIGVDRARVLTWVNNPLHAPYDDLIAADVDLSVATLTAIQKIAEAA 149 + EA+ LR G + + + D I L+ + Q A Sbjct: 57 ALLNLEEAITLRERGWKGPILMLEGFFHAQ-----DLEIYDQHRLTTCVHSNWQLKALQN 111 Query: 150 RAVGKPARVHIKVDTGFGRNGFTLAQIDSAIDLLLPLVDEGVFEIVGQWSHLAVADAPAV 209 + P +++KV++G R GF ++ + L + SH A A+ P Sbjct: 112 ARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQL---RAMANVGEMTLMSHFAEAEHP-- 166 Query: 210 GEFVEKTDEQIRVFEEFTSRLHARGIEPRIRHLANTAATLSRPEIHYDLVRPGVALYGYE 269 + + E+ L R L+N+AATL PE H+D VRPG+ LYG Sbjct: 167 ----DGISGAMARIEQAAEGL------ECRRSLSNSAATLWHPEAHFDWVRPGIILYGAS 216 Query: 270 ADPAMGTPSKYELRPAMTLQAQLSSVKNVGAGEGLSYGRVYVTDEPTSAAIVPVGYADGI 329 + LRP MTL +++ V+ + AGE + YG Y + IV GYADG Sbjct: 217 PSGQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY 276 Query: 330 HRSASGFNEAGSKHVNKPGAPVRVMTRDGARIFHVSGRVCMDQFILDLHGDAENMGVHIG 389 R A G PV V DG R G V MD +DL + IG Sbjct: 277 PRHAPT------------GTPVLV---DGVRT-MTVGTVSMDMLAVDLTPCPQ---AGIG 317 Query: 390 DTVELFGPGRGEDYLEPTADDWARAADTISYEIFTCLRNRVPRLY 434 VEL+G E DD A AA T+ YE+ L RVP + Sbjct: 318 TPVELWGK-------EIKIDDVAAAAGTVGYELMCALALRVPVVT 355

EN10_RS07900

EN10_RS07950

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
EN10_RS07900	-1	17	3.103708	hypothetical protein
EN10_RS07905	0	20	2.581998	dCTP deaminase
EN10_RS07910	-4	14	2.719325	cation-translocating P-type ATPase
EN10_RS07915	-3	12	1.818631	23S rRNA
EN10_RS07920	-3	11	1.314044	DUF4032 domain-containing protein
EN10_RS07925	-2	11	1.262814	sn-glycerol-3-phosphate ABC transporter
EN10_RS07930	-1	12	1.749773	potassium channel family protein
EN10_RS07935	0	13	1.541579	protein kinase
EN10_RS07940	-1	11	1.370615	thioredoxin domain-containing protein
EN10_RS07950	-1	13	1.707238	*G5 domain-containing protein

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
EN10_RS07900	CHANLCOLICIN	35	6e-04	Channel forming colicin signature.
>CHANLCOLICIN#Channel forming colicin signature. Length = 522 Score = 35.4 bits (81), Expect = 6e-04 Identities = 35/198 (17%), Positives = 67/198 (33%), Gaps = 11/198 (5%) Query: 291 AKTLDTLKSAVADAEKLDQQSVAECAVDANTAAIEKTTDSLRNTANEITKAKSALDAATK 350 A+T LK A A+ ++L S AV+ + + EI S L ++ Sbjct: 165 AETERQLKLAEAEEKRLAALSEEAKAVEIAQKKLSAAQSEVVKMDGEIKTLNSRLSSSIH 224 Query: 351 AVNDSKNMQSQSAARDELTKAIDDAQKILDASANAVTDEQVRTDLENRIADARKLADGQN 410 A M++ + R+EL +A +++ + R+ Sbjct: 225 A--RDAEMKTLAGKRNELAQASAKYKELDELVKKLSPRANDPLQNRPFFEATRRRVGAGK 282 Query: 411 LNAEDVTAMVKTLTDAGTAVQQSQTEYANQQAATAQQAAEEAQRQAEAQAQAAQQAQQNA 470 + E K +T + T + + + Q+A + A +A++N Sbjct: 283 IREE----KQKQVTASETRINRINADITQ-----IQKAISQVSNNRNAGIARVHEAEENL 333 Query: 471 AQAQQQNAQTTTPDTSDA 488 +AQ + D DA Sbjct: 334 KKAQNNLLNSQIKDAVDA 351
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
EN10_RS07925	PF05272	34	0.001	Virulence-associated E family protein
>PF05272#Virulence-associated E family protein Length = 892 Score = 34.3 bits (78), Expect = 0.001 Identities = 16/36 (44%), Positives = 20/36 (55%), Gaps = 1/36 (2%) Query: 34 LVLVGPSGCGKSTTLRMLAGLEEVNKGRILIG-GKD 68 +VL G G GKST + L GL+ + IG GKD Sbjct: 599 VVLEGTGGIGKSTLINTLVGLDFFSDTHFDIGTGKD 634
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
EN10_RS07930	PREPILNPTASE	29	0.016	Type IV prepilin cysteine protease (C20) family sig...
>PREPILNPTASE#Type IV prepilin cysteine protease (C20) family signature. Length = 290 Score = 29.4 bits (66), Expect = 0.016 Identities = 18/76 (23%), Positives = 37/76 (48%), Gaps = 10/76 (13%) Query: 157 SIWWAFVTVTT---VGYGDVYPVTTLG-----RFIAIILMITGIAVIGIVSAMISSWVID 208 S++WAF +T +GYGD + LG + + I+L+++ +++G + + + Sbjct: 197 SLYWAFKLLTGKEGMGYGDFKLLAALGAWLGWQALPIVLLLS--SLVGAFMGIGLILLRN 254 Query: 209 QVNVRPHPVIPAPAVG 224 +P P P A+ Sbjct: 255 HHQSKPIPFGPYLAIA 270
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
EN10_RS07935	YERSSTKINASE	40	4e-05	Yersinia serine/threonine protein kinase signature.
>YERSSTKINASE#Yersinia serine/threonine protein kinase signature. Length = 732 Score = 39.7 bits (92), Expect = 4e-05 Identities = 24/69 (34%), Positives = 37/69 (53%), Gaps = 8/69 (11%) Query: 133 LSSKLIDAVHAVHEAGIVHRDIKPTNVMISVTG--PVLVDFGIAMTPGESHVTSTGLVMG 190 ++ +L+D + + +AG+VH DIKP NV+ PV++D G+ GE G Sbjct: 250 IAHRLLDVTNHLAKAGVVHNDIKPGNVVFDRASGEPVVIDLGLHSRSGEQ---PKGF--- 303 Query: 191 TPGFIAPEV 199 T F APE+ Sbjct: 304 TESFKAPEL 312
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
EN10_RS07950	TONBPROTEIN	39	2e-05	Gram-negative bacterial tonB protein signature.
>TONBPROTEIN#Gram-negative bacterial tonB protein signature. Length = 239 Score = 39.2 bits (91), Expect = 2e-05 Identities = 17/89 (19%), Positives = 27/89 (30%), Gaps = 1/89 (1%) Query: 340 SAQPSQQPGAKPSEQPTSKPTQSAQPTQKPSEQPTQQPSAQPTQQPKPTPTPTQKPTQQP 399 + + P A + + +P +PKP P P +K +QP Sbjct: 51 TPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKPVKKVQEQP 110 Query: 400 -EQPTPSNCRLCKPSPSAAQAYAAGAAAQ 427 P R P + A A + A Sbjct: 111 KRDVKPVESRPASPFENTAPARLTSSTAT 139 Score = 31.1 bits (70), Expect = 0.008 Identities = 26/115 (22%), Positives = 34/115 (29%), Gaps = 6/115 (5%) Query: 319 SATENNTGDAAEPSAESSPTPSAQPSQQPGAKPSEQPTSKPTQSAQPTQKPSEQPTQQPS 378 S T D P A P + E P P +P KP +P Sbjct: 46 SVTMVTPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKPVKK 105 Query: 379 A--QPTQQPKPTPTPTQKP--TQQPEQPT--PSNCRLCKPSPSAAQAYAAGAAAQ 427 QP + KP + P P + T + KP S A A + Q Sbjct: 106 VQEQPKRDVKPVESRPASPFENTAPARLTSSTATAATSKPVTSVASGPRALSRNQ 160