PredictBias

identification of genomic and pathogenicity islands in prokaryotic genome

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A) Input parameters
Genome	sequence.gb	Threshold dinucleotide bias	2
Threshold codon bias	4	Threshold %GC bias	3
E-value (RPSBlast)	0.05	Genome (non-pathogenic)

B) Compare a potential GI or PAI in related non-pathogenic sp. (phylogenetic tree)
Potential GI or PAI start end
Select Organism

C) Potential GIs and PAIs in AL123456 (download)

S.No

Start

End

Bias

Virulence

Insertion elements

Prediction

Rv0018c

Rv0026

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
Rv0018c	2	12	0.734782	Phosphoserine/threonine phosphatase PstP
Rv0019c	1	11	0.848605	Conserved protein with FHA domain, FhaB
Rv0020c	2	12	1.092559	Conserved protein with FHA domain, FhaA
Rv0021c	0	13	0.769523	*Conserved hypothetical protein
Rv0022c	2	14	0.621921	Probable transcriptional regulatory protein
Rv0023	2	14	0.212702	Possible transcriptional regulatory protein
Rv0024	1	18	-0.001866	Putative secreted protein P60-related protein
Rv0025	3	19	-0.745629	Conserved hypothetical protein
Rv0026	2	18	-0.670123	Conserved hypothetical protein

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv0018c	IGASERPTASE	36	5e-04	IgA-specific serine endopeptidase (S6) signature.
>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature. Length = 1541 Score = 35.8 bits (82), Expect = 5e-04 Identities = 20/83 (24%), Positives = 25/83 (30%), Gaps = 7/83 (8%) Query: 426 APRATS--PPGRPAPPTTSETTEPNVTSSPASPSPTTSAPAPTGTTPAIPTSASPAAPAS 483 P+ TS P + T EP PT + P T + PA S Sbjct: 1122 VPKVTSQVSPKQEQSETVQPQAEPA-----RENDPTVNIKEPQSQTNTTADTEQPAKETS 1176 Query: 484 PPTPWPVTSSPTMAALPPPPPQP 506 PVT S T+ P Sbjct: 1177 SNVEQPVTESTTVNTGNSVVENP 1199
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv0019c	IGASERPTASE	28	0.016	IgA-specific serine endopeptidase (S6) signature.
>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature. Length = 1541 Score = 28.1 bits (62), Expect = 0.016 Identities = 14/55 (25%), Positives = 21/55 (38%) Query: 88 ADDSTLVLTDDYASTRHARLSMRGSEWYVEDLGSTNGTYLDRAKVTTAVRVPIGT 142 +D T +DY R + + S GTY D+ K VR+ G+ Sbjct: 154 TEDQTQKRREDYYMPRLDKFVTEVAPIEASTASSDAGTYNDQNKYPAFVRLGSGS 208

Rv0274

Rv0305c

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
Rv0274	5	17	5.346379	Conserved protein
Rv0275c	10	22	8.112180	Possible transcriptional regulatory protein
Rv0276	11	19	7.740593	Conserved hypothetical protein
Rv0277c	11	21	7.750325	Possible toxin VapC25. Contains PIN domain.
Rv0278c	6	15	6.101805	PE-PGRS family protein PE_PGRS3
Rv0279c	0	10	2.922633	PE-PGRS family protein PE_PGRS4
Rv0280	-4	9	0.904985	PPE family protein PPE3
Rv0281	-3	10	0.662373	Possible S-adenosylmethionine-dependent
Rv0282	-3	11	0.603737	ESX conserved component EccA3. ESX-3 type VII
Rv0283	-1	13	0.856522	ESX conserved component EccB3. ESX-3 type VII
Rv0284	-1	13	0.509853	ESX conserved component EccC3. ESX-3 type VII
Rv0285	4	20	1.591787	PE family protein PE5
Rv0286	3	16	1.920725	PPE family protein PPE4
Rv0287	2	15	2.077828	ESAT-6 like protein EsxG (conserved protein
Rv0288	1	13	1.400284	Low molecular weight protein antigen 7 EsxH (10
Rv0289	-1	13	1.443377	ESX-3 secretion-associated protein EspG3
Rv0290	1	13	1.421631	ESX conserved component EccD3. ESX-3 type VII
Rv0291	0	11	0.693454	Probable membrane-anchored mycosin MycP3 (serine
Rv0292	2	14	2.650896	ESX conserved component EccE3. ESX-3 type VII
Rv0293c	2	13	2.432154	Conserved protein
Rv0294	3	14	3.413526	Probable trans-aconitate methyltransferase Tam
Rv0295c	4	16	3.686454	Conserved protein
Rv0296c	5	15	3.664078	Probable sulfatase
Rv0297	4	18	4.894383	PE-PGRS family protein PE_PGRS5
Rv0298	1	17	-0.333013	Hypothetical protein
Rv0299	3	20	-3.589283	Hypothetical protein
Rv0300	4	18	-3.735906	Possible antitoxin VapB2
Rv0301	4	17	-3.536067	Possible toxin VapC2
Rv0302	4	18	-3.442211	Probable transcriptional regulatory protein
Rv0303	4	17	-3.665699	Probable dehydrogenase/reductase
Rv0304c	4	18	-4.016120	PPE family protein PPE5
Rv0305c	2	15	-2.984917	PPE family protein PPE6

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv0275c	HTHTETR	44	1e-07	TetR bacterial regulatory protein HTH signature.
>HTHTETR#TetR bacterial regulatory protein HTH signature. Length = 215 Score = 43.8 bits (103), Expect = 1e-07 Identities = 29/195 (14%), Positives = 60/195 (30%), Gaps = 13/195 (6%) Query: 14 AERLATRRRQSLSAGLDLLGSDQHDIAELTIRTICRRAGLSVRYFYESFTDKDEFVGRVF 73 + R+ L L L Q ++ ++ I + AG++ Y F DK + ++ Sbjct: 6 KQEAQETRQHILDVALRLF--SQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSEIW 63 Query: 74 DWVVAELVATTQAAVTAVPA--REQTRAGMANIVRTITADARVGRLL-FSTQLANAVITR 130 + + + P R + +++ + + R L+ V Sbjct: 64 ELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVTEERRRLLMEIIFHKCEFVGEM 123 Query: 131 -KRAESSALFAMLSGQHAVDTLHA-------PANDHVKAVAHFAVGGVGQTISAWLAGDV 182 ++ + S TL PA+ + A G + + WL Sbjct: 124 AVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMTRRAAIIMRGYISGLMENWLFAPQ 183 Query: 183 RLDPDQLVDQLAALL 197 D + A+L Sbjct: 184 SFDLKKEARDYVAIL 198
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv0278c	cloacin	44	2e-06	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 44.3 bits (104), Expect = 2e-06 Identities = 42/115 (36%), Positives = 53/115 (46%), Gaps = 3/115 (2%) Query: 117 NGANGAPGTGANGGDGGWLIGNGGAGGSGAAGVNGGAGGNGGAGGNGGAGGLIGNGGAGG 176 N + NGG G +G G + GSG + N GG G+G + G G GNGG G Sbjct: 10 NTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNG 69 Query: 177 AGGVASSGIGGSGGAGGNAMLFG--AGGAGGAGGGVVALTGGAGGAGGAGGNAGL 229 G SG GG+ A + FG A GAGG V+++ GA A A A L Sbjct: 70 NSG-GGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAAIADIMAAL 123 Score = 38.2 bits (88), Expect = 2e-04 Identities = 35/114 (30%), Positives = 41/114 (35%), Gaps = 6/114 (5%) Query: 143 GSGAAGVNGGAGGNGGAGGNGGAGGLIGNGGAGGAGGVASSGIGGSGGAGGNAMLFGAGG 202 G G N GA G NGG GL GGA G +S GG+G G G Sbjct: 3 GGDGRGHNTGAHSTSG-NINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 203 AGGAGGGVVALTGGAGGAGGAGGNAGLLFG-----AAGVGGAGGFTNGSALGGA 251 G GG + G G + A + FG G GG + AL A Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAA 115 Score = 36.2 bits (83), Expect = 7e-04 Identities = 38/104 (36%), Positives = 43/104 (41%), Gaps = 9/104 (8%) Query: 722 GTGGAGTNFGAGGNGGN--GGLFGAGGTGGAAGSGGSGITTGGGGHGGNAGLLSLGASGG 779 G G G N GA GN GG G G GGA+ G G G +G+ G SG Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 780 AGGSGGASSLAGGAGGTGGNG-----ALLFGFRGAGGAGGHGGA 818 G G +S GG GTGGN + FGF G G A Sbjct: 63 GNGGGNGNS--GGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 35.8 bits (82), Expect = 7e-04 Identities = 31/110 (28%), Positives = 41/110 (37%), Gaps = 3/110 (2%) Query: 687 AGGNGGLFANGGAGGAGGFNAAGGNGGNGGLFGTGGTGGAGTNFGAGGNGGNGGLFGAGG 746 +GG+G G +G N G GG G + N GG+G G G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 747 TGGAAGSGGSGITTGGGGHGGNAGL---LSLGASGGAGGSGGASSLAGGA 793 G G+G SG +G GG+ A G G A S++ GA Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGA 111 Score = 34.7 bits (79), Expect = 0.002 Identities = 34/111 (30%), Positives = 43/111 (38%), Gaps = 5/111 (4%) Query: 248 LGGAGGAGGAGGLFATGGV--GGSGGAGSSGGAGGAGGAGGL---FGAGGTGGHGGFADS 302 + G G G G +T G GG G G GGA G +G G G S Sbjct: 1 MSGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGS 60 Query: 303 SFGGVGGAGGAGGLFGAGGEGGSGGHSLVAGGDGGAGGNAGMLALGAAGGA 353 G GG G +GG G GG + + G + AG LA+ + GA Sbjct: 61 GHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGA 111 Score = 34.3 bits (78), Expect = 0.002 Identities = 21/71 (29%), Positives = 25/71 (35%) Query: 671 TGGHGAAGGVPAGVGGAGGNGGLFANGGAGGAGGFNAAGGNGGNGGLFGTGGTGGAGTNF 730 TG H +G + G G G GG G G G G+G G G Sbjct: 11 TGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNGN 70 Query: 731 GAGGNGGNGGL 741 GG+G G L Sbjct: 71 SGGGSGTGGNL 81 Score = 34.3 bits (78), Expect = 0.002 Identities = 31/87 (35%), Positives = 36/87 (41%), Gaps = 3/87 (3%) Query: 368 IGGAGGAGGNAGLLFGSGG-SGGAGGFGFADGGQGGPGGNAGTVFGSGGAGGNGGVGQGF 426 + G G G N G SG +GG G G G G G ++ GG+G G G Sbjct: 1 MSGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGS 60 Query: 427 AGGIGGAGGTPGLIGNGGNGGNGGASA 453 G GG G G G G GGN A A Sbjct: 61 GHGNGGGNGNSG--GGSGTGGNLSAVA 85 Score = 33.9 bits (77), Expect = 0.004 Identities = 34/110 (30%), Positives = 42/110 (38%), Gaps = 7/110 (6%) Query: 215 GGAGGAGGAGGNAGLLFGAAGVGGAGGFTNGSALGG-----AGGAGGAGGLFATGGVGGS 269 G GA GN G G+G GG ++GS GG+G G G Sbjct: 8 GHNTGAHSTSGNIN--GGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNG 65 Query: 270 GGAGSSGGAGGAGGAGGLFGAGGTGGHGGFADSSFGGVGGAGGAGGLFGA 319 GG G+SGG G GG A G + GG+ + AG L A Sbjct: 66 GGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAA 115 Score = 33.9 bits (77), Expect = 0.004 Identities = 37/134 (27%), Positives = 51/134 (38%) Query: 263 TGGVGGSGGAGSSGGAGGAGGAGGLFGAGGTGGHGGFADSSFGGVGGAGGAGGLFGAGGE 322 +GG G G+ +G G G GG G S GG G+G +G G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 323 GGSGGHSLVAGGDGGAGGNAGMLALGAAGGAGGIGGDGGTLTAGGIGGAGGAGGNAGLLF 382 G+GG + +GG G GGN +A A G + G A I + A ++ Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAAIADIMA 121 Query: 383 GSGGSGGAGGFGFA 396 G G +G A Sbjct: 122 ALKGPFKFGLWGVA 135 Score = 32.0 bits (72), Expect = 0.012 Identities = 37/113 (32%), Positives = 41/113 (36%), Gaps = 24/113 (21%) Query: 357 GGDGGTLTAGGIGGAGGAGGNAGLLFGSGGSGGAGGFGFADGGQGGPGGNAGTVFGSGGA 416 GGDG G GA GN +GG G G G GSG + Sbjct: 3 GGDG----RGHNTGAHSTSGN------------------INGGPTGLGVGGGASDGSGWS 40 Query: 417 GGNGGVGQGFAGGIGGAGGTPGLIGNGGNGGNGGASAVTGGNGGIGGTGVLIG 469 N G G GI GG+ GNGG GN G + TGGN V G Sbjct: 41 SENNPWGGGSGSGIHWGGGSGH--GNGGGNGNSGGGSGTGGNLSAVAAPVAFG 91 Score = 31.6 bits (71), Expect = 0.017 Identities = 33/103 (32%), Positives = 38/103 (36%), Gaps = 2/103 (1%) Query: 751 AGSGGSGITTGGGGHGGN--AGLLSLGASGGAGGSGGASSLAGGAGGTGGNGALLFGFRG 808 +G G G TG GN G LG GGA G SS GG G+G G G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 809 AGGAGGHGGAALTSIQQGGAGGAGGNGGLLFGSAGAGGAGGSG 851 G GG+G + S G F + GAGG Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 31.6 bits (71), Expect = 0.019 Identities = 36/116 (31%), Positives = 43/116 (37%), Gaps = 8/116 (6%) Query: 235 GVGGAGGFTNGSALGGAGGAGGAGGLFATGGVGGSGGAGSSGGAGGAGGAGGLFGAGGTG 294 G T+G+ GG G G GG A+ G G S GG G+G G G G Sbjct: 8 GHNTGAHSTSGNINGGPTGLGVGGG--ASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNG 65 Query: 295 GHGGFADSSFGGVGGAGGAGGLFGAGGEGGSGGHSLVAGGDGGAGGNAGMLALGAA 350 G G GG+G G L G +L G GG + AL AA Sbjct: 66 GGNG------NSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAA 115 Score = 30.8 bits (69), Expect = 0.027 Identities = 35/111 (31%), Positives = 43/111 (38%), Gaps = 4/111 (3%) Query: 290 AGGTG-GHGGFADSSFGGVGGAGGAGGLFGAGGEGGSGGHSLVAGGDGGAGGNAGMLALG 348 +GG G GH A S+ G + G G+ G G GSG S GG+G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGV-GGGASDGSGWSSENNPWGGGSGSGIHWGGGS 60 Query: 349 AAGGAGGIGGDGGTLTAGGIGGAGGAGGNAGLLFGSGGSGGAGGFGFADGG 399 G GG G GG GG A A G F + + GAGG + Sbjct: 61 GHGNGGGNGNSGGGSGTGGNLSAVAAPVAFG--FPALSTPGAGGLAVSISA 109 Score = 30.8 bits (69), Expect = 0.030 Identities = 33/104 (31%), Positives = 42/104 (40%), Gaps = 5/104 (4%) Query: 575 GNGGNGGHGATNTAATATGGAGGAGGILFGTGGNGGTGGIATGAGGIGGAGGAGGVSLLI 634 G+G GA +T+ GG G G G G + G+G + GG G+G Sbjct: 4 GDGRGHNTGAHSTSGNINGGPTGLGV---GGGASDGSGWSSENNPW-GGGSGSGIHWGGG 59 Query: 635 GSGGTGGNGGNSIGVAGIGGAGGRGGDAGLLFGAAGTGGHGAAG 678 G GG GNS G +G GG A + FG GA G Sbjct: 60 SGHGNGGGNGNSGGGSGTGG-NLSAVAAPVAFGFPALSTPGAGG 102 Score = 30.8 bits (69), Expect = 0.030 Identities = 28/93 (30%), Positives = 35/93 (37%), Gaps = 2/93 (2%) Query: 338 AGGNAGMLALGAAGGAGGIGGDGGTLTAGGIGGAGGAGGNAGLLFGSGGSGGAGGFGFAD 397 +GG+ GA +G I G L GG G + +G G G G + Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 398 GGQGGPGGNAGTVFGSGGAGGNGGVGQGFAGGI 430 G GG GN+G GSG G V A G Sbjct: 62 HGNGGGNGNSGG--GSGTGGNLSAVAAPVAFGF 92 Score = 30.5 bits (68), Expect = 0.039 Identities = 29/95 (30%), Positives = 38/95 (40%), Gaps = 9/95 (9%) Query: 779 GAGGSGGASSLAGGAGGTGGNGALLFGFRGAGGAGGHGGAALTSIQQGGAGGAGGNGGLL 838 G G + GA S +G G G G GG + S + GG G+G Sbjct: 6 GRGHNTGAHSTSGNINGGPT---------GLGVGGGASDGSGWSSENNPWGGGSGSGIHW 56 Query: 839 FGSAGAGGAGGSGANALGAGTGGTGGDGGHAGVFG 873 G +G G GG+G + G+GTGG FG Sbjct: 57 GGGSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFG 91
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv0279c	cloacin	38	1e-04	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 38.2 bits (88), Expect = 1e-04 Identities = 31/102 (30%), Positives = 41/102 (40%), Gaps = 4/102 (3%) Query: 117 NGTNGAPGTGANGGDGGWLIGNGGAGGSGAAGVN----GGAGGNGGAGGLIGNGGAGGAG 172 N + NGG G +G G + GSG + N GG+G GG G+G GG G Sbjct: 10 NTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNG 69 Query: 173 GRASTGTGGAGGAGGAAGMLFGAAGVGGPGGFAAAFGATGGA 214 G + AA + FG + PG A + GA Sbjct: 70 NSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGA 111 Score = 36.6 bits (84), Expect = 4e-04 Identities = 32/107 (29%), Positives = 45/107 (42%), Gaps = 4/107 (3%) Query: 628 GTGGLFANGGAGGAGGFGANGGTGGNGLLFGAGGTGGAGTLGADGGAGGHGGLFGAGGTG 687 G G N GA G G TG + G+G + GG G G +G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 688 GAGGSSGGTFGGNGGSGGNAGLLALGASG----GAGGSGGSALNVGG 730 G GG +G + GG+G G + + A A G G+GG A+++ Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISA 109 Score = 35.8 bits (82), Expect = 6e-04 Identities = 30/82 (36%), Positives = 38/82 (46%) Query: 283 AGGTGGSGGTSIINVGGNGGAGGDAGMLSLGAAGGAGGSGGSNPDGGGGAGGIGGDGGTL 342 +GG G T + GN G + GA+ G+G S +NP GGG GI GG+ Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 343 FGSGGAGGVCGLGFDAGGAGGA 364 G+GG G G G GG A Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSA 83 Score = 34.7 bits (79), Expect = 0.002 Identities = 26/78 (33%), Positives = 33/78 (42%) Query: 693 SGGTFGGNGGSGGNAGLLALGASGGAGGSGGSALNVGGTGGVGGNGGSGGSLFGFGGAGG 752 SGG G+ + G G G GG++ G + GG GS +GG G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 753 TGGSSGIGSSGGTGGDGG 770 G G G+SGG G GG Sbjct: 62 HGNGGGNGNSGGGSGTGG 79 Score = 33.9 bits (77), Expect = 0.003 Identities = 29/110 (26%), Positives = 40/110 (36%), Gaps = 2/110 (1%) Query: 211 TGGAGGAGGNGGLFADGGVGGAGGATDAGTGGAGGSG--GNGGLFGAGGTGGPGGFGIFG 268 +GG G G G + G G G + GSG +G G G G G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 269 GGAGGDGGSGGLFGAGGTGGSGGTSIINVGGNGGAGGDAGMLSLGAAGGA 318 G GG G+ G G S + + G + AG L++ + GA Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGA 111 Score = 33.5 bits (76), Expect = 0.003 Identities = 32/95 (33%), Positives = 37/95 (38%), Gaps = 9/95 (9%) Query: 682 GAGGTGGAGGSSGGTFGGNGGSGGNAGLLALGASGGAGGSGGSALNVGGTGGVGGNGGSG 741 G G GA +SG GG G G G AS G+G S + GG+G GG Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGG-----ASDGSGWSSENNPWGGGSGSGIHWGGGS 60 Query: 742 GSLFGFGGAGGTGGSSGIGSSGGTGGDGGTAGVFG 776 G G GG G S G +GG FG Sbjct: 61 GH----GNGGGNGNSGGGSGTGGNLSAVAAPVAFG 91 Score = 32.8 bits (74), Expect = 0.006 Identities = 33/105 (31%), Positives = 41/105 (39%), Gaps = 1/105 (0%) Query: 659 AGGTGGAGTLGADGGAGGHGGLFGAGGTGGAGGSSGGTFGGNGGSGGNAGLLALGASGGA 718 +GG G GA +G G G GG G N GG +G G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 719 GGSGGSALNVGGTGGVGGNGGSGGSLFGFG-GAGGTGGSSGIGSS 762 G+GG N GG G GGN + + FG A T G+ G+ S Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVS 106 Score = 32.8 bits (74), Expect = 0.007 Identities = 31/93 (33%), Positives = 37/93 (39%), Gaps = 2/93 (2%) Query: 377 AGGAGGGSFAGAGGTGGA--GGAPGLVGNAGNGGNGGASANGAGAAGGAGGSGVLIGNGG 434 +GG G G GA T G GG GL G G S+ GG+G G G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 435 NGGSGGTGAPAGTAGAGGLGGQLLGRDGFNAPA 467 +G GG G G +G GG + F PA Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPA 94 Score = 32.4 bits (73), Expect = 0.008 Identities = 32/120 (26%), Positives = 38/120 (31%), Gaps = 12/120 (10%) Query: 219 GNGGLFADGGVGGAGGATDAGTGGAGGSGGNGGLFGAGGTGGPGGFGIFGGGAGGDGGSG 278 G G + G G + G G G GG G P G GG G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWG--------GGSGSGI 54 Query: 279 GLFGAGGTGGSGGTSIINVGGNGGAGGDAGMLSLGAAGGAGGSGGSNPDGGGGAGGIGGD 338 G G G GG N GG+G + ++ A G S P GG A I Sbjct: 55 HWGGGSGHGNGGG----NGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAG 110 Score = 32.4 bits (73), Expect = 0.009 Identities = 32/101 (31%), Positives = 38/101 (37%) Query: 575 SGGTGGSGGFGLDTGGAGGRGGDAGLFLGAAGTGGQAALSQNFIGAGGTAGAGGTGGLFA 634 SGG G G + GG GL +G + G S+N GG+ GG Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 635 NGGAGGAGGFGANGGTGGNGLLFGAGGTGGAGTLGADGGAG 675 +G GG G G GTGGN A G L G G Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG 102 Score = 32.0 bits (72), Expect = 0.010 Identities = 37/132 (28%), Positives = 46/132 (34%), Gaps = 16/132 (12%) Query: 242 GAGGSGGNGGLFGAGGT--GGPGGFGIFGGGAGGDGGSGGLFGAGGTGGSGGTSIINVGG 299 G G G N G G GGP G G+ GG + G G S GG GSG I+ GG Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSG----IHWGG 58 Query: 300 NGGAGGDAGMLSLGAAGGAGGSGGSNPDGGGGAGGIGGDGGTLFGSGGAGGVCGLGFDAG 359 G G GG G+ G GG + F + G GL Sbjct: 59 GSGHGN----------GGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSIS 108 Query: 360 GAGGAGGKAGLL 371 + A ++ Sbjct: 109 AGALSAAIADIM 120 Score = 32.0 bits (72), Expect = 0.011 Identities = 39/135 (28%), Positives = 47/135 (34%), Gaps = 10/135 (7%) Query: 139 GGAGGSGAAGVNGGAGGNGGAGGLIGNGGAGGAGGRASTGTGGAGGAGGAAGMLFGAAGV 198 G G + NGG GL GGA G +S GG+G G+ Sbjct: 4 GDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHG 63 Query: 199 GGPGGFAAAFGATGGAGGAGGNGGLFADGGVGGAGGATDAGTGGAGGSGGNGGLFGA--- 255 G G G +GG G GGN A G + G GG S G L A Sbjct: 64 NGGGN-----GNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAAIAD 118 Query: 256 --GGTGGPGGFGIFG 268 GP FG++G Sbjct: 119 IMAALKGPFKFGLWG 133 Score = 32.0 bits (72), Expect = 0.013 Identities = 31/114 (27%), Positives = 39/114 (34%), Gaps = 5/114 (4%) Query: 171 AGGRASTGTGGAGGAGGAAGMLFGAAGVGGPGGFAAAFGATGGAGGAGGNGGLFADGGVG 230 +GG GA G GVGG + + + G G G+ GG G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 231 GAGGATDAGTGGAGGSGGNGGLFGAGGTGGPGGFGIFGGGAGGDGGSGGLFGAG 284 G + +GG G+GGN A P FG G GG AG Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAA-----PVAFGFPALSTPGAGGLAVSISAG 110 Score = 31.2 bits (70), Expect = 0.017 Identities = 35/110 (31%), Positives = 44/110 (40%), Gaps = 14/110 (12%) Query: 619 GAGGTAGAGGTGGLFANGGAGGAGGFGANGGTG----------GNGLLFGAGGTGGAGTL 668 G G GA T G G G G GA+ G+G G+G GG G G Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNG 65 Query: 669 GADGGAGGHGGLFGAGGTGGAGGSSGGTFGGNGGSGGNAGLLALGASGGA 718 G +G +GG G G + ++ FG S AG LA+ S GA Sbjct: 66 GGNGNSGGGSG----TGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGA 111 Score = 31.2 bits (70), Expect = 0.018 Identities = 25/79 (31%), Positives = 33/79 (41%), Gaps = 3/79 (3%) Query: 353 GLGFDAGGAGGAGGKAGLLIGAGGAGGAGGGSFAGAGGT---GGAGGAPGLVGNAGNGGN 409 G G + G +G G G G GGA GS + GG+G G +G+G Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNG 65 Query: 410 GGASANGAGAAGGAGGSGV 428 GG +G G+ G S V Sbjct: 66 GGNGNSGGGSGTGGNLSAV 84 Score = 30.5 bits (68), Expect = 0.036 Identities = 34/121 (28%), Positives = 41/121 (33%), Gaps = 5/121 (4%) Query: 640 GAGGFGANGGTGGNGLLFGAGGTGGAGTLGADGGAG-GHGGLFGAGGTGGAGGSSGGTFG 698 G G G N G G TG GA G+G GG+G GG+ Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 699 GNGGSGGNAGLLALGASGGAGGSGGSALNVGGTGGVGGNGGSGGSLFGFGGAGGTGGSSG 758 GNGG GN+G G SG G A V G+GG + + Sbjct: 63 GNGGGNGNSG----GGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAAIAD 118 Query: 759 I 759 I Sbjct: 119 I 119 Score = 30.1 bits (67), Expect = 0.041 Identities = 33/113 (29%), Positives = 40/113 (35%), Gaps = 2/113 (1%) Query: 531 GGDGGSGGAGGILSGIGGTGGSGGIGTTGQGGTGGTGGAALLIGSGGTG-GSGGFGLDTG 589 GGDG G + GG G+G G G + GG+G G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 590 GAGGRGGDAGLFLGAAGTGGQAALSQNF-IGAGGTAGAGGTGGLFANGGAGGA 641 G GG G++G G G A F A T GAGG + G A Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAA 115
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv0284	TYPE3IMPPROT	34	0.003	Type III secretion system inner membrane P protein ...
>TYPE3IMPPROT#Type III secretion system inner membrane P protein family signature. Length = 224 Score = 33.6 bits (77), Expect = 0.003 Identities = 18/64 (28%), Positives = 33/64 (51%), Gaps = 13/64 (20%) Query: 27 PPELPRVIPP---SLLRRALPYLIGILI------VGMIVA--LVATGMRVISPQTLFFPF 75 P + ++P S ++ A + IG + V ++V+ L+A GM ++SP T+ P Sbjct: 140 KPSIFALLPAYALSEIKSA--FKIGFYLYLPFVVVDLVVSSVLLALGMMMMSPVTISTPI 197 Query: 76 VLLL 79 L+L Sbjct: 198 KLVL 201
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv0286	PF03544	29	0.043	Gram-negative bacterial tonB protein
>PF03544#Gram-negative bacterial tonB protein Length = 243 Score = 28.8 bits (64), Expect = 0.043 Identities = 19/126 (15%), Positives = 27/126 (21%), Gaps = 13/126 (10%) Query: 295 IVLAPAVIPPASTPLAAAAVAAGSVWPAVSMAVTGAGTAGAATPAAGAAPSAGAAPAPAA 354 ++APA + P P A V P A P P P Sbjct: 53 TMVAPADLEP---PQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKPVK 109 Query: 355 PATASFAYAVGGSGDWGPSLGPTVGGRGGIKAPAATVPAAAAAAATRGQ----------S 404 T R A + A+ + + Sbjct: 110 KVEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQPQYPA 169 Query: 405 RARRRR 410 RA+ R Sbjct: 170 RAQALR 175
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv0291	SUBTILISIN	156	4e-46	Subtilisin serine protease family (S8) signature.
>SUBTILISIN#Subtilisin serine protease family (S8) signature. Length = 326 Score = 156 bits (397), Expect = 4e-46 Identities = 83/334 (24%), Positives = 123/334 (36%), Gaps = 70/334 (20%) Query: 74 MLNLPAAWQFSRGEGQLVAIIDTGVQPG-PRLP-NVDAGGDFVESTDG----LTDCDGHG 127 M+ PA W +RG G VA++DTG P L + G +F + +G D +GHG Sbjct: 28 MIQAPAVWNQTRGRGVKVAVLDTGCDADHPDLKARIIGGRNFTDDDEGDPEIFKDYNGHG 87 Query: 128 TLVAGIVAGQPGNDGFSGVAPAARLLSIRAMSTKFSPRTSGGDPQLAQATLDVAVLAGAI 187 T VAG +A +G GVAP A LL I+ ++ + S + + I Sbjct: 88 THVAGTIAATENENGVVGVAPEADLLIIKVLNKQGSGQYDW--------------IIQGI 133 Query: 188 VHAADLGAKVINVSTITCLPADRMVDQAALGAAIRYAAVDKDAVIVAAAGNTGASGSVSA 247 +A + +I++S D L A++ AV +++ AAGN G Sbjct: 134 YYAIEQKVDIISMS------LGGPEDVPELHEAVKK-AVASQILVMCAAGNEGDGDD-RT 185 Query: 248 SCDSNPLTDLSRPDDPRNWAGVTSVSIPSWWQPYVLSVASLTSAGQPSKFSMPGPWVGIA 307 P V+SV ++ S+FS V + Sbjct: 186 DELGYP-----------------------GCYNEVISVGAINFDRHASEFSNSNNEVDLV 222 Query: 308 APGENIASVSNSGDGALANGLPDAHQKLVALSGTSYAAGYVSGVAALVRSRYPG-----L 362 APGE+I S G K SGTS A +V+G AL++ L Sbjct: 223 APGEDILSTVPGG-------------KYATFSGTSMATPHVAGALALIKQLANASFERDL 269 Query: 363 NATEVVRRLTATAHRGARESSNIVGAGNLDAVAA 396 E+ +L S + G G L A Sbjct: 270 TEPELYAQLIKRTIPLGN-SPKMEGNGLLYLTAV 302
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv0294	BCTLIPOCALIN	27	0.044	Bacterial lipocalin signature.
>BCTLIPOCALIN#Bacterial lipocalin signature. Length = 171 Score = 27.3 bits (60), Expect = 0.044 Identities = 16/59 (27%), Positives = 21/59 (35%), Gaps = 3/59 (5%) Query: 17 PFYELVSRVGLERARRVVDLGCGPGHLTRYLARRWPGAVIEALDSSPEMVAAAAERGID 75 PFY L+R GP +L R P LD + + + ERG D Sbjct: 106 PFYGSYVVFELDRENYSYAFVSGPNTEYLWLLSRTPTVERGILD---KFIEMSKERGFD 161
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv0297	cloacin	37	1e-04	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 37.4 bits (86), Expect = 1e-04 Identities = 36/97 (37%), Positives = 39/97 (40%), Gaps = 2/97 (2%) Query: 149 GQAGGAGGAAGFFGNGGNGGDGGAGANGGAGGTAGWFFGFGGNGGAGGIGVAGINGGLGG 208 G G A NGG G G GGA +GW GG G G+ GG G Sbjct: 4 GDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIH--WGGGSG 61 Query: 209 AGGDGGNAGFFGNGGNGGMGGAGAAGVNAVNPGLATP 245 G GGN G G GG A AA V P L+TP Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTP 98 Score = 35.5 bits (81), Expect = 6e-04 Identities = 26/77 (33%), Positives = 31/77 (40%) Query: 282 GAGGDGGNASTSGGIGIAQTGGAGGAGGAGGDGAPGGNGGNGGSVEHTGATGSSASGGNG 341 G G + G STSG I TG G G + G G N GG G + GNG Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNG 65 Query: 342 ATGGNGGVGAPGGAGGN 358 GN G G+ G + Sbjct: 66 GGNGNSGGGSGTGGNLS 82 Score = 35.1 bits (80), Expect = 8e-04 Identities = 28/90 (31%), Positives = 33/90 (36%) Query: 488 GGDGGGRGIFGQFGAGGAGGAGGVGGAGGAGGTGGGGGNGGAIFNAGTPGAAGTGGDGGV 547 GGDG G +G G G GG G G + + G+ GG G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 548 GGTGAAGGKGGAGGSGGVNGATGADGAKGL 577 G G G GG G+GG A A A G Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFGF 92 Score = 33.1 bits (75), Expect = 0.003 Identities = 32/115 (27%), Positives = 41/115 (35%), Gaps = 4/115 (3%) Query: 263 GTAGGGADGANGSAIGQAGGAGGDGGNASTSGGIGIAQTGGAGGAGGAGGDGAPGGNGGN 322 G G GA+ ++ GG G G S G G + G G G GG+G Sbjct: 4 GDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHG 63 Query: 323 GGSVEHTGATGSSASGGNGATGGNGGVGAPG----GAGGNGGHVSGGSVNTAGAG 373 G GS G A G P GAGG +S G+++ A A Sbjct: 64 NGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAAIAD 118 Score = 33.1 bits (75), Expect = 0.003 Identities = 32/101 (31%), Positives = 36/101 (35%), Gaps = 9/101 (8%) Query: 453 GAGAAGAIGGHGGDGGSVNTPIGGSEAGDGGKGGLG--------GDGGGRGIFGQFGAGG 504 G G G G++N G G G G G G G G GI G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGI-HWGGGSG 61 Query: 505 AGGAGGVGGAGGAGGTGGGGGNGGAIFNAGTPGAAGTGGDG 545 G GG G +GG GTGG A G P + G G Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG 102 Score = 32.4 bits (73), Expect = 0.005 Identities = 27/83 (32%), Positives = 33/83 (39%) Query: 377 GNGGTGGAGGPGGHGGSVLSGPVGDSGNGGAGGDGGAGVSATDIAGTGGRGGNGGHGGLW 436 G G G G G++ GP G GGA G G G G + G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 437 IGNGGDGGAGGVGGVGGAGAAGA 459 GG+G +GG G GG +A A Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVA 85 Score = 32.4 bits (73), Expect = 0.006 Identities = 33/107 (30%), Positives = 41/107 (38%), Gaps = 6/107 (5%) Query: 355 AGGNGGHVSGGSVNTAGAGGKGGNGGTGGAGGPGGHGGSVLSGPVGDSGNGGAGGDGGAG 414 +GG+G + G+ +T+G G G G G G G S + P G G GG+G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 415 VSATDIAGTGGRGGNGGHGGLWIGNGGDGGAGGVGGVGGAGAAGAIG 461 G GG GN G G GN A G GA G Sbjct: 62 ------HGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG 102 Score = 31.2 bits (70), Expect = 0.012 Identities = 25/77 (32%), Positives = 34/77 (44%) Query: 321 GNGGSVEHTGATGSSASGGNGATGGNGGVGAPGGAGGNGGHVSGGSVNTAGAGGKGGNGG 380 G G +TGA +S + G TG G GA G+G + + G + +G GG+G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 381 TGGAGGPGGHGGSVLSG 397 G G GGS G Sbjct: 63 GNGGGNGNSGGGSGTGG 79 Score = 30.1 bits (67), Expect = 0.029 Identities = 21/78 (26%), Positives = 29/78 (37%) Query: 309 GAGGDGAPGGNGGNGGSVEHTGATGSSASGGNGATGGNGGVGAPGGAGGNGGHVSGGSVN 368 G G G G G++ G + +G + GG G+G H GGS + Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 369 TAGAGGKGGNGGTGGAGG 386 G G GG+G G Sbjct: 63 GNGGGNGNSGGGSGTGGN 80 Score = 30.1 bits (67), Expect = 0.031 Identities = 30/105 (28%), Positives = 38/105 (36%), Gaps = 3/105 (2%) Query: 206 LGGAGGDGGNAGFFGNGGN--GGMGGAGAAGVNAVNPGLATPVTPAANGGNGLNLVGVPG 263 + G G G N G GN GG G G G + G ++ P GG+G + G Sbjct: 1 MSGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWG-GGSGSGIHWGGG 59 Query: 264 TAGGGADGANGSAIGQAGGAGGDGGNASTSGGIGIAQTGGAGGAG 308 + G G S G G A + G T GAGG Sbjct: 60 SGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 30.1 bits (67), Expect = 0.033 Identities = 32/98 (32%), Positives = 35/98 (35%), Gaps = 3/98 (3%) Query: 118 NGANGAPGTGQAGGDG-GLLFGNGGNGGSGAPGQAGGAGGAAGFFGNGGNGGDGGAGANG 176 NG G G DG G N GG G G G G G GN G G Sbjct: 21 NGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNGNSGGGSGTGGN 80 Query: 177 GAGGTAGWFFGFGG--NGGAGGIGVAGINGGLGGAGGD 212 + A FGF GAGG+ V+ G L A D Sbjct: 81 LSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAAIAD 118
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv0302	HTHTETR	84	5e-22	TetR bacterial regulatory protein HTH signature.
>HTHTETR#TetR bacterial regulatory protein HTH signature. Length = 215 Score = 83.5 bits (206), Expect = 5e-22 Identities = 43/207 (20%), Positives = 78/207 (37%), Gaps = 11/207 (5%) Query: 5 AKKKQQQGERSRESILDATERLMATKGYAATSISDIRDACGLAPSSIYWHFGSKEGVLAA 64 A+K +Q+ + +R+ ILD RL + +G ++TS+ +I A G+ +IYWHF K + + Sbjct: 2 ARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSE 61 Query: 65 MMERGAQRFFAAIP-TWDEAHGPVEQRSERQLTELVSLQSQHPDFLRLFYLLSMERSQDP 123 + E + G L ++ + + RL + + + Sbjct: 62 IWELSESNIGELELEYQAKFPGDPLSVLREILIHVLES-TVTEERRRLLMEIIFHKCEFV 120 Query: 124 AVAAVVRRVRNTAIARFRDSITHLLPSDIPPG--KADLVVAELTAFAVALSDGVYFAGHL 181 AVV++ + D I L I ADL+ G+ Sbjct: 121 GEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMTRRAAIIMRGYISGLMENWLF 180 Query: 182 EPDTTDVERMYRRLRQALEALIPVLLE 208 P + D L++ + +LLE Sbjct: 181 APQSFD-------LKKEARDYVAILLE 200
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv0303	DHBDHDRGNASE	40	4e-06	2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase signature. Length = 261 Score = 40.4 bits (94), Expect = 4e-06 Identities = 50/209 (23%), Positives = 76/209 (36%), Gaps = 35/209 (16%) Query: 6 AVITGASSGLGLQCARALLRRDASWHVVLAVRDPARGRAAMEELGEPNRCS-VLEVDLAS 64 A ITGA+ G+G AR L + A H+ +P + + L R + D+ Sbjct: 11 AFITGAAQGIGEAVARTLASQGA--HIAAVDYNPEKLEKVVSSLKAEARHAEAFPADVRD 68 Query: 65 VRSVRSFVETVRTTPLPPIRALVCNAGLQVVSGI-AFTDDGVEMTFGVNHLGHFALVTGI 123 ++ + + PI LV AG+ I + +D+ E TF VN G F + Sbjct: 69 SAAIDEITARI-EREMGPIDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGVFNASRSV 127 Query: 124 LDWLA--RPARIVVVSSGTHDPSKHTGMPDPRYTCAADLAHPPTDQNTPAEGRRRYTTSK 181 ++ R IV V S +P+ P Y +SK Sbjct: 128 SKYMMDRRSGSIVTVGS---NPA-----------------------GVPRTSMAAYASSK 161 Query: 182 LCNVLFTYELDRRLDHGEQGVMVNAFDPG 210 V+FT L L+ E + N PG Sbjct: 162 AAAVMFTKCLG--LELAEYNIRCNIVSPG 188
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv0305c	cloacin	32	0.009	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 32.4 bits (73), Expect = 0.009 Identities = 25/84 (29%), Positives = 31/84 (36%), Gaps = 8/84 (9%) Query: 247 GHNIGFANTGSNNVGFGNTGSNNVGIGLTGNGQIGFGSFNSGSHNIGLFNSGSGNVGLFN 306 GHN G +T GN G+G+ G G G + + G SG G Sbjct: 8 GHNTGAHSTS------GNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGG--G 59 Query: 307 SGTGNFGIGNSGTGNFGLGNTGST 330 SG GN G + G G G S Sbjct: 60 SGHGNGGGNGNSGGGSGTGGNLSA 83

Rv0345

Rv0355c

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
Rv0345	2	10	-2.913992	Conserved hypothetical protein
Rv0346c	3	11	-2.930682	Possible L-asparagine permease AnsP2
Rv0347	3	12	-1.946527	Probable conserved membrane protein
Rv0348	3	12	-1.607512	Possible transcriptional regulatory protein
Rv0349	4	13	-1.386554	Hypothetical protein
Rv0350	6	21	-3.248704	Probable chaperone protein DnaK (heat shock
Rv0351	7	22	-3.147163	Probable GrpE protein (HSP-70 cofactor)
Rv0352	6	22	-3.066628	Probable chaperone protein DnaJ1
Rv0353	6	22	-3.064487	Probable heat shock protein transcriptional
Rv0354c	6	21	-2.828755	PPE family protein PPE7
Rv0355c	6	20	-2.628713	PPE family protein PPE8

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv0350	SHAPEPROTEIN	130	2e-35	Bacterial cell shape determinant MreB/Mbl protein s...
>SHAPEPROTEIN#Bacterial cell shape determinant MreB/Mbl protein signature. Length = 347 Score = 130 bits (329), Expect = 2e-35 Identities = 71/368 (19%), Positives = 141/368 (38%), Gaps = 66/368 (17%) Query: 2 ARAVGIDLGTTNSVVSVLEGGDP-----VVVANSEGSRTTPSIVAFARNGEVLVGQPAKN 56 + + IDLGT N+++ V G VV + + + S+ A VG AK Sbjct: 10 SNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQDRAGSPKSVAA--------VGHDAKQ 61 Query: 57 QAVTNVD--RTVRSVKRHMGSDWSIEIDGKKYTAPEISARILMKLKRDAEAYLGEDITDA 114 +R +K + +D+ + ++ + ++ ++ Sbjct: 62 MLGRTPGNIAAIRPMKDGVIADF--------FVTEKMLQHFIKQVHSNS---FMRPSPRV 110 Query: 115 VITTPAYFNDAQRQATKDAGQIAGLNVLRIVNEPTAAALAYGLDKGEKEQRILVFDLGGG 174 ++ P +R+A +++ Q AG + ++ EP AAA+ GL E +V D+GGG Sbjct: 111 LVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG-SMVVDIGGG 169 Query: 175 TFDVSLLEIGEGVVEVRATSGDNHLGGDDWDQRVVDWLVDKFKGTSGIDLTKDKMAMQRL 234 T +V+++ + V S +GGD +D+ +++++ + G Sbjct: 170 TTEVAVISLNGVV-----YSSSVRIGGDRFDEAIINYVRRNYGSLIG------------- 211 Query: 235 REAAEKAKIELSSS----QSTSINLPYITVDAD--KNPLFLDEQLTRAEFQRITQDL--- 285 AE+ K E+ S+ + I + + + ++ A + +T + Sbjct: 212 EATAERIKHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAV 271 Query: 286 ---LDRTRKPFQSVIADTGISVSEIDHVVLVGGSTRMPAVTDLVKELTGGKEPNKGVNPD 342 L++ S I++ G+ VL GG + + L+ E T G +P Sbjct: 272 MVALEQCPPELASDISERGM--------VLTGGGALLRNLDRLLMEET-GIPVVVAEDPL 322 Query: 343 EVVAVGAA 350 VA G Sbjct: 323 TCVARGGG 330
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv0355c	cloacin	35	0.004	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 35.5 bits (81), Expect = 0.004 Identities = 25/78 (32%), Positives = 31/78 (39%), Gaps = 2/78 (2%) Query: 267 NTGSNNIGFGNTGDGNRGIGLTGSGLLGFGGLNSGTGNIGLFNSGTGNVGIGNSGTGNWG 326 NTG+++ GN G G+G+ G G G + G SG G G G Sbjct: 10 NTGAHSTS-GNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGN 68 Query: 327 IGNSGNSYNTGFGNSGDA 344 GNSG TG S A Sbjct: 69 -GNSGGGSGTGGNLSAVA 85 Score = 32.4 bits (73), Expect = 0.035 Identities = 23/78 (29%), Positives = 33/78 (42%), Gaps = 4/78 (5%) Query: 301 GTGNIGLFNSGTGNVGIGNSGTGNWGIGNSGNSYNTGFGNSGDANTGFFNSGIANTGVGN 360 G G+ +S +GN+ G +G G G S +G+ + + G SGI G Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLG----VGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 361 AGNYNTGSYNPGNSNTGG 378 GN + G S TGG Sbjct: 62 HGNGGGNGNSGGGSGTGG 79 Score = 32.0 bits (72), Expect = 0.050 Identities = 24/81 (29%), Positives = 31/81 (38%), Gaps = 2/81 (2%) Query: 3094 GNIGFGNTGNGNIGIGNTGTGNIGFGNTGNGNIGIGLTGDTMTGFGGWNSGTGNIGLFNS 3153 G G G+ + GN G G G G + G G + + GG SG G S Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGG--GS 60 Query: 3154 GTGNIGFGNSGTGNWGIGNSG 3174 G GN G + G G G + Sbjct: 61 GHGNGGGNGNSGGGSGTGGNL 81

Rv0371c

Rv0378

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
Rv0371c	2	14	1.956561	Conserved hypothetical protein
Rv0372c	2	13	1.768614	Conserved hypothetical protein
Rv0373c	2	12	1.774134	Probable carbon monoxyde dehydrogenase (large
Rv0374c	3	15	2.647095	Probable carbon monoxyde dehydrogenase (small
Rv0375c	3	14	3.042250	Probable carbon monoxyde dehydrogenase (medium
Rv0376c	5	13	2.740581	Conserved hypothetical protein
Rv0377	2	12	2.355426	Probable transcriptional regulatory protein
Rv0378	3	13	2.560658	Conserved hypothetical glycine rich protein

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv0371c	ACRIFLAVINRP	29	0.016	Acriflavin resistance protein family signature.
>ACRIFLAVINRP#Acriflavin resistance protein family signature. Length = 1034 Score = 28.7 bits (64), Expect = 0.016 Identities = 16/48 (33%), Positives = 24/48 (50%), Gaps = 3/48 (6%) Query: 33 VLGATLDVARQAGFDQLILTLGGAASAVRAAMALDGTDVVVVEDVERG 80 VL T + G+ LT+ G A+ + +D +VVVE+VER Sbjct: 375 VLLGTFAILAAFGYSINTLTMFGMVLAI--GLLVDDA-IVVVENVERV 419

Rv0399c

Rv0405

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
Rv0399c	-2	15	-4.539874	Possible conserved lipoprotein LpqK
Rv0400c	-1	22	-5.858374	Acyl-CoA dehydrogenase FadE7
Rv0401	-1	25	-6.331013	Probable conserved transmembrane protein
Rv0402c	-1	23	-6.318652	Probable conserved transmembrane transport
Rv0403c	0	24	-5.658286	Probable conserved membrane protein MmpS1
Rv0404	0	23	-5.206176	Fatty-acid-AMP ligase FadD30 (fatty-acid-AMP
Rv0405	-2	14	-3.118354	Probable membrane bound polyketide synthase

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv0402c	ACRIFLAVINRP	51	2e-08	Acriflavin resistance protein family signature.
>ACRIFLAVINRP#Acriflavin resistance protein family signature. Length = 1034 Score = 51.0 bits (122), Expect = 2e-08 Identities = 48/281 (17%), Positives = 102/281 (36%), Gaps = 44/281 (15%) Query: 146 QGGSQANESVAAVQRIVDSVPP--PPGIKAYVTGPGPLGADRVVYGDRSLHTIT---GIS 200 G+ A ++ A++ + + P P G+K D + S+H + + Sbjct: 294 ATGANALDTAKAIKAKLAELQPFFPQGMKVLYP------YDTTPFVQLSIHEVVKTLFEA 347 Query: 201 IAVIAIMLFIAYRSLSAALIMLLTVGLELLAVRGIISTFAVNDLMGLSTFTVNVL----V 256 I ++ +++++ +++ A LI + V + LL TFA+ G +++N L + Sbjct: 348 IMLVFLVMYLFLQNMRATLIPTIAVPVVLLG------TFAILAAFG---YSINTLTMFGM 398 Query: 257 ALTIAASTDYIIFLVGRYQEARATG--QNREAAYYTMFGGTAHVVLASGLTVAGA---MY 311 L I D I +V + +EA +M ++ + ++ M Sbjct: 399 VLAIGLLVDDAIVVVENVERVMMEDKLPPKEATEKSM-SQIQGALVGIAMVLSAVFIPMA 457 Query: 312 CLGFTRLPYFNTLASPCAIGLVTVMLASLTLAPAIIAVASR-------------FGLFDP 358 G + + + + +L +L L PA+ A + FG F+ Sbjct: 458 FFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATLLKPVSAEHHENKGGFFGWFNT 517 Query: 359 KRATTKRRWRRIGTVVVRWPGPVLAATLLIALIGLLALPKY 399 + + ++ G L LI + G++ L Sbjct: 518 TFDHSVNHYTNSVGKILGSTGRYLLIYALI-VAGMVVLFLR 557 Score = 37.9 bits (88), Expect = 2e-04 Identities = 31/167 (18%), Positives = 63/167 (37%), Gaps = 11/167 (6%) Query: 758 EGTLYDVMIAVVASLCLIFIIMLGITRSVVASAVIVGTVALSLGSAFGLSVLIWQHILHM 817 + + A++ L+F++M +++ A+ + V + L F + I + Sbjct: 338 HEVVKTLFEAIM----LVFLVMYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINTL 393 Query: 818 PLHWLVLPMAIIVMLAVGSDYNLLLIARFQEEIGAGLKTGMIRAMAGTGRVVTIAGLVFA 877 + +VL + ++V A+ N + R E K ++M+ + +V + Sbjct: 394 TMFGMVLAIGLLVDDAIVVVEN---VERVMMEDKLPPKEATEKSMSQIQGALVGIAMVLS 450 Query: 878 FTMGSMVA---SDLRVVGQIGTTIMIGLLFDTLVVRSYMTPALATLL 921 M S + Q TI+ + LV +TPAL L Sbjct: 451 AVFIPMAFFGGSTGAIYRQFSITIVSAMALSVLVALI-LTPALCATL 496 Score = 35.2 bits (81), Expect = 0.002 Identities = 36/215 (16%), Positives = 78/215 (36%), Gaps = 19/215 (8%) Query: 145 DQGGSQANESVAAVQRIVDSVPPPPGIKAYVTGPGPLGADRVVYGDRSLHTITGISIAVI 204 G+ + +++A ++ + +P G TG + + + IS V+ Sbjct: 830 AAPGTSSGDAMALMENLASKLPAGIGYD--WTG----MSYQERLSGNQAPALVAISFVVV 883 Query: 205 AIMLFIAYRSLSAALIMLLTVGLELLAVRGIISTFAVNDLMGLSTFTVNVLVALTIAAST 264 + L Y S S + ++L V L ++ +++ N + F V +L + ++A Sbjct: 884 FLCLAALYESWSIPVSVMLVVPLGIVG--VLLAATLFNQKNDV-YFMVGLLTTIGLSAKN 940 Query: 265 DYIIFLVGRYQEA-RATGQNREAAYYTMFGGTAHV--VLASGLTVAGAMYCLGFTRLP-- 319 I +V ++ G+ A T+ + +L + L + L + Sbjct: 941 A--ILIVEFAKDLMEKEGKGVVEA--TLMAVRMRLRPILMTSLAFILGVLPLAISNGAGS 996 Query: 320 -YFNTLASPCAIGLVTVMLASLTLAPAIIAVASRF 353 N + G+V+ L ++ P V R Sbjct: 997 GAQNAVGIGVMGGMVSATLLAIFFVPVFFVVIRRC 1031 Score = 31.7 bits (72), Expect = 0.019 Identities = 55/319 (17%), Positives = 105/319 (32%), Gaps = 55/319 (17%) Query: 635 TVKDLAQTLTSAFSGL-VTQMEDMTRNATVMGRTFDAANNDDSFYLPPEAFQN-PDFQRG 692 ++ D+ QT+++A G V D R + + D F + PE Sbjct: 741 SLSDINQTISTALGGTYVNDFIDRGRVKKLYVQA------DAKFRMLPEDVDKLYVRSAN 794 Query: 693 -----LKLFLSPDGTCARFVITHR-GDPAS------AEGISHIDPIMQAADEAVKGTPLQ 740 F + + G P+ A G S M + P Sbjct: 795 GEMVPFSAFTTSHWVYGSPRLERYNGLPSMEIQGEAAPGTS-SGDAMALMENLASKLP-- 851 Query: 741 AASIYLAGTSSTYKDIHEGTLYDVMIAVVASLCLIFIIMLGITRSVVASAVIVGTVALSL 800 A I T +Y++ G V S ++F+ + + S ++ V L + Sbjct: 852 -AGIGYDWTGMSYQERLSGN--QAPALVAISFVVVFLCLAALYESWSIPVSVMLVVPLGI 908 Query: 801 GSAFGLSVLIWQHILHMPLHWLVLPMAIIVMLAVG-SDYNLLLIARFQEEIGAGLKTGMI 859 + VL+ + + + + ++ +G S N +LI F +++ G++ Sbjct: 909 -----VGVLLAATLFNQKND---VYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGVV 960 Query: 860 RAMAGTGRV----------VTIAG---LVFAFTMGSMVASDLRVVGQIGTTIMIGLLFDT 906 A R+ I G L + GS + +G +M G++ T Sbjct: 961 EATLMAVRMRLRPILMTSLAFILGVLPLAISNGAGSGAQN------AVGIGVMGGMVSAT 1014 Query: 907 LVVRSYMTPALATLLGRWF 925 L+ + P ++ R F Sbjct: 1015 LLAI-FFVPVFFVVIRRCF 1032

Rv0459

Rv0474

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
Rv0459	2	10	-2.043777	Conserved hypothetical protein
Rv0460	1	9	-2.105590	Conserved hydrophobic protein
Rv0461	1	10	-2.106451	Probable transmembrane protein
Rv0462	0	14	-1.761245	Dihydrolipoamide dehydrogenase LpdC (lipoamide
Rv0463	1	15	-1.159799	Probable conserved membrane protein
Rv0464c	0	11	-2.936096	Conserved protein
Rv0465c	1	13	-3.300722	Probable transcriptional regulatory protein
Rv0466	1	13	-3.634470	Conserved protein
Rv0467	0	12	-3.165518	Isocitrate lyase Icl (isocitrase)
Rv0468	-1	11	-4.205602	3-hydroxybutyryl-CoA dehydrogenase FadB2
Rv0469	-1	11	-3.669285	Possible mycolic acid synthase UmaA
Rv0470c	0	11	-1.769494	Mycolic acid synthase PcaA (cyclopropane
Rv0470A	2	15	-0.670041	Hypothetical protein
Rv0471c	2	16	-1.073676	Hypothetical protein
Rv0472c	3	15	-1.408955	Probable transcriptional regulatory protein
Rv0473	2	17	-0.129948	Possible conserved transmembrane protein
Rv0474	3	13	-0.735107	Probable transcriptional regulatory protein

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv0461	OMPADOMAIN	27	0.034	OMPA domain signature.
>OMPADOMAIN#OMPA domain signature. Length = 346 Score = 27.2 bits (60), Expect = 0.034 Identities = 11/26 (42%), Positives = 16/26 (61%) Query: 135 TALSLGVMSGPFASVAAAAPLYGYYY 160 TA+++ V FA+VA AAP +Y Sbjct: 4 TAIAIAVALAGFATVAQAAPKDNTWY 29
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv0472c	HTHTETR	52	3e-10	TetR bacterial regulatory protein HTH signature.
>HTHTETR#TetR bacterial regulatory protein HTH signature. Length = 215 Score = 51.9 bits (124), Expect = 3e-10 Identities = 34/182 (18%), Positives = 66/182 (36%), Gaps = 14/182 (7%) Query: 17 RRWHQHKVERRNELVDGTIEAIRRHGRF-LSMDEIAAEIGVSKTVLYRYFVDKNDLTTAV 75 R+ Q E R ++D + + G S+ EIA GV++ +Y +F DK+DL + + Sbjct: 3 RKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSEI 62 Query: 76 MMRFTQTTLIPNMIAALSADMDG--FELTREIIRVYVETVAAQPEPYRFVMANSSASKS- 132 I + A G + REI+ +E+ + + + Sbjct: 63 WELSESN--IGELELEYQAKFPGDPLSVLREILIHVLESTVTEERRRLLMEIIFHKCEFV 120 Query: 133 ---KVIADSERIIA---RMLAVMLRRRMQEAGMDTGGVEP--WAYLIVGGVQLATHSWMS 184 V+ ++R + + EA M + A ++ G + +W+ Sbjct: 121 GEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMTRRAAIIMRGYISGLMENWLF 180 Query: 185 DP 186 P Sbjct: 181 AP 182

Rv0514

Rv0538

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
Rv0514	2	13	0.481006	Possible transmembrane protein
Rv0515	2	12	-0.049525	Conserved 13E12 repeat family protein
Rv0516c	2	9	-0.887253	Possible anti-anti-sigma factor
Rv0517	1	9	-1.385650	Possible membrane acyltransferase
Rv0518	0	11	-1.252211	Possible exported protein
Rv0519c	-2	10	0.124361	Possible conserved membrane protein
Rv0520	-2	11	-0.011010	Possible methyltransferase/methylase (fragment)
Rv0521	-3	9	0.554746	Possible methyltransferase/methylase (fragment)
Rv0522	-2	11	1.237359	Probable GABA permease GabP (4-amino butyrate
Rv0523c	0	17	3.262597	Conserved protein
Rv0524	1	18	3.305392	Probable glutamate-1-semialdehyde
Rv0525	2	18	3.242475	Conserved protein
Rv0526	3	18	3.057469	Possible thioredoxin protein (thiol-disulfide
Rv0527	2	20	3.149059	Possible cytochrome C-type biogenesis protein
Rv0528	4	16	5.299946	Probable conserved transmembrane protein
Rv0529	3	13	4.684634	Possible cytochrome C-type biogenesis protein
Rv0530	4	14	4.996711	Conserved protein
Rv0530A	6	15	4.650663	Conserved protein
Rv0531	6	15	4.820329	Possible conserved membrane protein
Rv0532	4	14	4.140309	PE-PGRS family protein PE_PGRS6
Rv0533c	0	13	2.709558	3-oxoacyl-[acyl-carrier-protein] synthase III
Rv0534c	1	14	3.336018	1,4-dihydroxy-2-naphthoate octaprenyltransferase
Rv0535	0	13	3.280387	Probable 5'-methylthioadenosine phosphorylase
Rv0536	-1	15	3.458648	Probable UDP-glucose 4-epimerase GalE3
Rv0537c	-1	14	3.263654	Probable integral membrane protein
Rv0538	-1	14	3.619127	Possible conserved membrane protein

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv0532	cloacin	42	7e-06	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 41.6 bits (97), Expect = 7e-06 Identities = 29/85 (34%), Positives = 35/85 (41%) Query: 375 LSSGDGGAGGAGGGGGWLFGNGGDGGAGGGGGGRFGSGSGAGGDGAVGGAGGAGAWFGNG 434 +S GDG G NGG G G GGG GSG + + GG+G W G Sbjct: 1 MSGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGS 60 Query: 435 GAGGVGGGGGRGTTAIGGDGGAGGA 459 G G GG G G + G + A Sbjct: 61 GHGNGGGNGNSGGGSGTGGNLSAVA 85 Score = 37.0 bits (85), Expect = 2e-04 Identities = 29/97 (29%), Positives = 33/97 (34%) Query: 344 GGAAGAGGAGGWLYGDGGAGGVGGVGGAVFSLSSGDGGAGGAGGGGGWLFGNGGDGGAGG 403 G GA G + G GVGG SS + GG G G G G G GG Sbjct: 8 GHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGG 67 Query: 404 GGGGRFGSGSGAGGDGAVGGAGGAGAWFGNGGAGGVG 440 G GSG+G GAGG+ Sbjct: 68 NGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 37.0 bits (85), Expect = 2e-04 Identities = 24/77 (31%), Positives = 36/77 (46%) Query: 138 GNGGNGGSGAAGQAGGAGGAAGLIGHGGTGGAVTGVSTTGGPGGHGGDAGLYGFGGAGGA 197 G+G +GA +G G +G GG +G S+ P G G +G++ GG+G Sbjct: 4 GDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHG 63 Query: 198 GGFGQSGAAGGAGGAGG 214 G G + GG+G G Sbjct: 64 NGGGNGNSGGGSGTGGN 80 Score = 36.2 bits (83), Expect = 4e-04 Identities = 33/100 (33%), Positives = 37/100 (37%), Gaps = 1/100 (1%) Query: 358 GDGGAGGVGGVGGAVFSLSSGDGGAGGAGGGGGWLFGNGGDGGAGGGGGGRFGSGSGAGG 417 G G G G + +G G GGA G GW N GG G G G GSG G Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGG-GSGHGN 64 Query: 418 DGAVGGAGGAGAWFGNGGAGGVGGGGGRGTTAIGGDGGAG 457 G G +GG GN A G + G GG Sbjct: 65 GGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 35.8 bits (82), Expect = 5e-04 Identities = 24/80 (30%), Positives = 28/80 (35%) Query: 413 SGAGGDGAVGGAGGAGAWFGNGGAGGVGGGGGRGTTAIGGDGGAGGAGGAGGWLYGDGGA 472 SG G G GA G G GGG + + G G G +G G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 473 GGAGGGGGRGGTGNDGGDGG 492 G GGG G G G+ G Sbjct: 62 HGNGGGNGNSGGGSGTGGNL 81 Score = 35.8 bits (82), Expect = 5e-04 Identities = 34/103 (33%), Positives = 45/103 (43%), Gaps = 3/103 (2%) Query: 204 GAAGGAGGAGGWLYGDGGDGGAGDNGGNESGTGVSAVGGVGGAGGAGGLLFGNGGDGGVG 263 G GA G + +GG G G GG G+G S+ G G G+ +G G G G Sbjct: 8 GHNTGAHSTSGNI--NGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNG 65 Query: 264 GDGGDGSSTQDSGGDGGAGGAGGAGGW-LLGNGGAGGAGGAAS 305 G G+ +GG+ A A A G+ L GAGG + S Sbjct: 66 GGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSIS 108 Score = 34.7 bits (79), Expect = 0.001 Identities = 25/77 (32%), Positives = 28/77 (36%) Query: 410 GSGSGAGGDGAVGGAGGAGAWFGNGGAGGVGGGGGRGTTAIGGDGGAGGAGGAGGWLYGD 469 G G G G G G GG G G GG G+G G G Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNG 65 Query: 470 GGAGGAGGGGGRGGTGN 486 GG G +GGG G GG + Sbjct: 66 GGNGNSGGGSGTGGNLS 82 Score = 34.7 bits (79), Expect = 0.001 Identities = 33/107 (30%), Positives = 40/107 (37%) Query: 435 GAGGVGGGGGRGTTAIGGDGGAGGAGGAGGWLYGDGGAGGAGGGGGRGGTGNDGGDGGDG 494 G G G G +T+ +GG G G GG G G + GG G+G G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 495 GRGGDAQLLGNGGDGGAGGAGGPAGLALPPGPARPAGAAVPAVRCSA 541 G GG G G G + A +A GA AV SA Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISA 109 Score = 32.4 bits (73), Expect = 0.005 Identities = 29/108 (26%), Positives = 33/108 (30%), Gaps = 1/108 (0%) Query: 153 GAGGAAGLIGHGGTGGAVTGVSTTGGPGGHGGDAGLYGFGGAGGAGGFGQSGAAGGAGGA 212 G G G T G + G T G GG D + GG G GG G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 213 GGWLYGDGGDGGAGDNGGNESGTGVSAVGGVG-GAGGAGGLLFGNGGD 259 G GG+G G + A G GAGGL Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAG 110 Score = 32.0 bits (72), Expect = 0.007 Identities = 32/94 (34%), Positives = 35/94 (37%), Gaps = 6/94 (6%) Query: 235 TGVSAVGGVGGAGGAGGLLFGNGGDGGVGGDGGDG---SSTQDSGGDGGAGGAGGAGGWL 291 +G G GA G + G GVGG DG SS + G G G GG Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 292 LGNGGAGGAGGAASIKVATGGLGGDGGDAGLFGF 325 GNGG G G S TGG FGF Sbjct: 62 HGNGGGNGNSGGGS---GTGGNLSAVAAPVAFGF 92 Score = 31.2 bits (70), Expect = 0.014 Identities = 29/103 (28%), Positives = 33/103 (32%), Gaps = 3/103 (2%) Query: 326 GGDGGWGGRGVDARFGAAGGAAGAGGAGGWLYGDGGAGGVGGVGGAVFSLSSGDGGAGGA 385 GGDG G + G G G GG G G GG G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 386 GGGGGWLFGNGGDGGAGGGGGGRFGSGSGAGGDGAVGGAGGAG 428 G GGG GG+G GG + A G A+ G G Sbjct: 63 GNGGG---NGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG 102 Score = 30.1 bits (67), Expect = 0.027 Identities = 35/102 (34%), Positives = 40/102 (39%), Gaps = 4/102 (3%) Query: 256 NGGDGGVGGDGGDGSSTQDSGGDGGAGGAGGAG---GWLLGNGGAGGAGGAASIKVATGG 312 +GGDG G +S +GG G G GGA GW N GG G+ I G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGS-GIHWGGGS 60 Query: 313 LGGDGGDAGLFGFGGDGGWGGRGVDARFGAAGGAAGAGGAGG 354 G+GG G G G G V A A GAGG Sbjct: 61 GHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG 102 Score = 29.7 bits (66), Expect = 0.039 Identities = 40/124 (32%), Positives = 47/124 (37%), Gaps = 5/124 (4%) Query: 194 AGGAGGFGQSGAAGGAGGAGGWLYGDGGDGGAGDNGG----NESGTGVSAVGGVGGAGGA 249 +GG G +GA +G G G G GGA D G N G S G G G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 250 GGLLFGNGGDGGVGGDGGDGSSTQDSGGDG-GAGGAGGAGGWLLGNGGAGGAGGAASIKV 308 G GNG GG G GG+ S+ G A GAGG + + A I Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAAIADIMA 121 Query: 309 ATGG 312 A G Sbjct: 122 ALKG 125
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv0536	NUCEPIMERASE	171	9e-53	Nucleotide sugar epimerase signature.
>NUCEPIMERASE#Nucleotide sugar epimerase signature. Length = 334 Score = 171 bits (434), Expect = 9e-53 Identities = 91/369 (24%), Positives = 132/369 (35%), Gaps = 74/369 (20%) Query: 1 MRVLLTGAAGFIGSRVDAALRAAGHDVVGVDALLPAAHGPNPVLPPGCQ----------- 49 M+ L+TGAAGFIG V L AGH VVG+D L + L Sbjct: 1 MKYLVTGAAGFIGFHVSKRLLEAGHQVVGIDNLND---YYDVSLKQARLELLAQPGFQFH 57 Query: 50 RVDVRDASALAPLLA--GVDLVCHQAAMVGAGVNAADAPAYGGHNDFATTVLLAQMFAAG 107 ++D+ D + L A + V + + + AY N +L Sbjct: 58 KIDLADREGMTDLFASGHFERVFISPHRLAVRYSLENPHAYADSNLTGFLNILEGCRHNK 117 Query: 108 VRRLVLASSMVVYGQGRYDCPQHGPVDPLPRRRADLDNGVFEHRCPGCGEPVIWQLVDED 167 ++ L+ ASS VYG R +P F +D Sbjct: 118 IQHLLYASSSSVYGLNR----------KMP----------FST---------------DD 142 Query: 168 APLRPRSLYAASKTAQEHYALAWSEASGGSVVALRYHNVYGPGMPRDTPYSGVAAIFRSA 227 + P SLYAA+K A E A +S G LR+ VYGP D F A Sbjct: 143 SVDHPVSLYAATKKANELMAHTYSHLYGLPATGLRFFTVYGPWGRPDMALF----KFTKA 198 Query: 228 VEKGKPPKVFEDGGQMRDFVHVDDVAAANLAAVH---LGEADRDGFTA-----------V 273 + +GK V+ G RDF ++DD+A A + + T Sbjct: 199 MLEGKSIDVYNYGKMKRDFTYIDDIAEAIIRLQDVIPHADTQWTVETGTPAASIAPYRVY 258 Query: 274 NVCSGRPISILQVATAICDARGGSMSPAITGH--YRSGDVRHIVADPARAARVLGFRAAV 331 N+ + P+ ++ A+ DA G A + GDV AD V+GF Sbjct: 259 NIGNSSPVELMDYIQALEDALG---IEAKKNMLPLQPGDVLETSADTKALYEVIGFTPET 315 Query: 332 DPGEGLREF 340 +G++ F Sbjct: 316 TVKDGVKNF 324
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv0538	IGASERPTASE	39	8e-05	IgA-specific serine endopeptidase (S6) signature.
>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature. Length = 1541 Score = 38.5 bits (89), Expect = 8e-05 Identities = 25/113 (22%), Positives = 34/113 (30%), Gaps = 6/113 (5%) Query: 402 PGWQPGMPTIPTAPPTTPVTTSATTPPTTPPTTPVTTPPTTPPTTPVTTPPTTPPTTPVT 461 P +P PT P + + TT T P T PVT T V Sbjct: 1141 PQAEPARENDPTVNIKEPQSQTNTTADTEQPAK--ETSSNVEQ--PVTESTTVNTGNSVV 1196 Query: 462 TPPTTVAPTTVAPTTVAPTTVAPTTVAPAT--ATPTTVAPQPTQQPTQQPTQQ 512 P P T PT + ++ P + + P V P T + Sbjct: 1197 ENPENTTPATTQPTVNSESSNKPKNRHRRSVRSVPHNVEPATTSSNDRSTVAL 1249 Score = 33.1 bits (75), Expect = 0.003 Identities = 19/89 (21%), Positives = 25/89 (28%), Gaps = 2/89 (2%) Query: 439 PPTTPPTTPVTTPPTTPPTTPVTTPPTTVAPTTVAPTTVAPTTVAPTTVAPATATPTTVA 498 P T +P T P P PT + T T PA T + V Sbjct: 1123 PKVTSQVSPKQEQSET--VQPQAEPARENDPTVNIKEPQSQTNTTADTEQPAKETSSNVE 1180 Query: 499 PQPTQQPTQQPTQQMPTQQQTVAPQTVAP 527 T+ T + + P T P Sbjct: 1181 QPVTESTTVNTGNSVVENPENTTPATTQP 1209

Rv0573c

Rv0586

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
Rv0573c	3	17	5.124063	Nicotinic acid phosphoribosyltransferase PncB2
Rv0574c	3	16	5.383552	Conserved hypothetical protein
Rv0575c	3	18	5.846628	Possible oxidoreductase
Rv0576	5	17	6.559067	Probable transcriptional regulatory protein
Rv0577	6	20	6.983439	Conserved protein TB27.3
Rv0578c	5	20	7.016630	PE-PGRS family protein PE_PGRS7
Rv0579	1	11	-0.924088	Conserved hypothetical protein
Rv0580c	-1	12	-1.047327	Conserved protein
Rv0581	-2	11	-1.228777	Possible antitoxin VapB26
Rv0582	-2	11	-1.416914	Possible toxin VapC26. Contains PIN domain.
Rv0583c	-2	10	-1.509364	Probable conserved lipoprotein LpqN
Rv0584	-2	10	-2.006327	Possible conserved exported protein
Rv0585c	-2	12	-2.417612	Probable conserved integral membrane protein
Rv0586	0	10	-3.245774	Probable transcriptional regulatory protein

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv0578c	FLAGELLIN	42	1e-05	Flagellin signature.
>FLAGELLIN#Flagellin signature. Length = 507 Score = 42.3 bits (99), Expect = 1e-05 Identities = 43/296 (14%), Positives = 62/296 (20%), Gaps = 14/296 (4%) Query: 904 NGDHALSGNGAAGGNGGNGGNGSLRGSGGAGGHGGNGGNASRGMGGDGGTGGAGGNAGQI 963 NG LS + G ++ S G+ G G G + Sbjct: 132 NGVKVLSQDNQMKIQVGANDGETITID------LQKIDVKSLGLDGFNVNGPKEATVGDL 185 Query: 964 GNGGAGGNGGDGGTGSDGNPGAITGSGGRGGDGGVGGQGGSVAGDGADGGRGGAGGTGGT 1023 + G D SG D V + A+G T Sbjct: 186 KSSFKNVTGYDTYAVGANKYRVDVNSGAVVTDTTAPTVPDKVYVNAANGQLTTDDAENNT 245 Query: 1024 GLRGTTGATGATGTFDAGADGHGGNGGTGGVGGT---GGAGGGGGNGGAGGKALSPTGNN 1080 + GT +A A GG G G G +S T N Sbjct: 246 AVDLFKTTKSTAGTAEAKAIAGAIKGGKEGDTFDYKGVTFTIDTKTGNDGNGKVSTTING 305 Query: 1081 GSQGAGGDGGAGGAGGTGGTGGDGGRGAHGTLFSSLAGTGGTGGNGGTGGTGGTGGAGGA 1140 GA + + + G + T A Sbjct: 306 EKVTLTVADITAGAANVDAATLQSSKNVYTS-----VVNGQFTFDDKTKNESAKLSDLEA 360 Query: 1141 GGTGSTLGATGATGAAGRAGNGGVGGSGGLGSAFGPGGTGGMGGAGGTSTVSAGGD 1196 GA A G + + F G+ +A Sbjct: 361 NNAVKGESKITVNGAEYTANAAGDKVTLAGKTMFIDKTASGVSTLINEDAAAAKKS 416
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv0581	SHAPEPROTEIN	24	0.040	Bacterial cell shape determinant MreB/Mbl protein s...
>SHAPEPROTEIN#Bacterial cell shape determinant MreB/Mbl protein signature. Length = 347 Score = 24.3 bits (53), Expect = 0.040 Identities = 10/32 (31%), Positives = 18/32 (56%) Query: 13 KAAVKRAARQRGVSEAQVIRESIRAAVGGAKP 44 + A++ +A+ G E +I E + AA+G P Sbjct: 123 RRAIRESAQGAGAREVFLIEEPMAAAIGAGLP 154

Rv0737

Rv0755c

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
Rv0737	2	16	1.637740	Possible transcriptional regulatory protein
Rv0738	3	15	0.982612	Conserved protein
Rv0739	1	14	-0.093875	Conserved hypothetical protein
Rv0740	0	18	0.870737	Conserved hypothetical protein
Rv0741	7	15	7.202271	Probable transposase (fragment)
Rv0742	12	18	9.564406	PE-PGRS family protein PE_PGRS8
Rv0743c	10	18	9.257948	Hypothetical protein
Rv0744c	9	18	9.596683	Possible transcriptional regulatory protein
Rv0745	11	19	10.129693	Conserved hypothetical protein
Rv0746	12	23	10.359504	PE-PGRS family protein PE_PGRS9
Rv0747	9	22	7.417849	PE-PGRS family protein PE_PGRS10
Rv0748	1	18	0.769523	Possible antitoxin VapB31
Rv0749	0	16	0.860349	Possible toxin VapC31. Contains PIN domain.
Rv0749A	1	13	1.589767	Hypothetical protein (fragment)
Rv0750	4	11	-0.558499	Conserved hypothetical protein
Rv0751c	4	11	-0.743195	Probable 3-hydroxyisobutyrate dehydrogenase MmsB
Rv0752c	4	9	-0.789772	Probable acyl-CoA dehydrogenase FadE9
Rv0753c	3	9	-1.351954	Probable methylmalonate-semialdehyde
Rv0754	3	12	-1.345947	PE-PGRS family protein PE_PGRS11
Rv0755c	3	14	-2.758797	PPE family protein PPE12

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv0746	cloacin	36	6e-04	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 35.8 bits (82), Expect = 6e-04 Identities = 26/79 (32%), Positives = 30/79 (37%) Query: 514 GGAGGAAGLWGTGGAGGAGGSSAGGGGAGGAGGAGGWLLGDGGAGGIGGASTVLGGTGGG 573 GG G +G G G G GGA GW + GG G+ GG G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 574 GGVGGLWGAGGAGGAGGTG 592 G GG +GG G GG Sbjct: 63 GNGGGNGNSGGGSGTGGNL 81 Score = 35.1 bits (80), Expect = 0.001 Identities = 29/103 (28%), Positives = 40/103 (38%), Gaps = 2/103 (1%) Query: 272 TGGRGFLNNGGVGGAGGN--AGLLFGAGGTGGSGGAGLGGDGGAGGAGGNTGVLFGNAGS 329 +GG G +N G GN G G G S G+G + G G +G+ +G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 330 GGTGGFGDTDGGAGGAGGDAGWLGSGGVGGAGGFGETGDGGVG 372 G GG GG G GG+ + + G G GG+ Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 34.3 bits (78), Expect = 0.002 Identities = 24/91 (26%), Positives = 36/91 (39%), Gaps = 9/91 (9%) Query: 232 GAGGHGGAGGLGAVTGGVGGTGGAGGLLAGLLAGPGGAGGTGGRGFLNNGGVGGAGGNAG 291 G G G G + +G + G G G G + G G+ + G G +G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLG---------VGGGASDGSGWSSENNPWGGGSGSG 53 Query: 292 LLFGAGGTGGSGGAGLGGDGGAGGAGGNTGV 322 + +G G G+GG GG+G G + V Sbjct: 54 IHWGGGSGHGNGGGNGNSGGGSGTGGNLSAV 84 Score = 33.9 bits (77), Expect = 0.003 Identities = 35/134 (26%), Positives = 49/134 (36%), Gaps = 3/134 (2%) Query: 588 AGGTGLVGGDGGAGGAGGTGGLLAGLIGAGGGHGGTGGLSTNGDGGVGGAGGNAGMLAGP 647 +GG G G +G G GL GG G+G S N G G G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 648 GGAGGAGGDGENLDTGGDGGAGGSAGLLFG--SGGAGGAGGFGF-LGGDGGAGGNAGLLL 704 G GG G+ G + +A + FG + GAGG + + A ++ Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAAIADIMA 121 Query: 705 SSGGAGGFGGFGTA 718 + G FG +G A Sbjct: 122 ALKGPFKFGLWGVA 135 Score = 33.5 bits (76), Expect = 0.004 Identities = 29/95 (30%), Positives = 38/95 (40%), Gaps = 2/95 (2%) Query: 478 NGTPGAVGSGATGAPGGWLLGDGGAGGSGAAGSGAPGGAGGAAGLWGTGGAGGAGGSSAG 537 N + G P G +G G + GSG + P G G +G+ GG+G G G Sbjct: 10 NTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNG--GG 67 Query: 538 GGGAGGAGGAGGWLLGDGGAGGIGGASTVLGGTGG 572 G +GG G GG L G + G GG Sbjct: 68 NGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG 102 Score = 32.8 bits (74), Expect = 0.005 Identities = 38/122 (31%), Positives = 42/122 (34%), Gaps = 20/122 (16%) Query: 302 SGGAGLGGDGGAGGAGGNTGVLFGNAGSGGTGGFGDTDGGAGGAGGDAGWLGSGGVGGAG 361 SGG G G + GA GN N G G G GGA +GW G G Sbjct: 2 SGGDGRGHNTGAHSTSGNI-----NGGPTGLGV-------GGGASDGSGWSSENNPWGGG 49 Query: 362 GFGETGDGGVGGAGGKAGLLIGNGGAGGAGGQGAVTGGTGGAGGDGVLIGNGGNAGIGGT 421 GG G G NGG G G G+ TGG A V G + G Sbjct: 50 SGSGIHWGGGSGHG--------NGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAG 101 Query: 422 GP 423 G Sbjct: 102 GL 103 Score = 32.4 bits (73), Expect = 0.009 Identities = 30/106 (28%), Positives = 39/106 (36%) Query: 128 NGANGAPGTGANGAPGGWLLGNGGAGGSAAAGSGLPGGAGGAAGLFGTGGAGGAGGSSTV 187 N + NG P G +G G + GS + P G G +G+ GG+G G Sbjct: 10 NTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNG 69 Query: 188 GDGEAGGAGGSGGWLLGTGGVGGVGGLGAGAGGAGGVGGAGGLLGA 233 G G GG+ + G GAGG AG L A Sbjct: 70 NSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAA 115 Score = 32.4 bits (73), Expect = 0.009 Identities = 24/68 (35%), Positives = 25/68 (36%) Query: 174 GTGGAGGAGGSSTVGDGEAGGAGGSGGWLLGTGGVGGVGGLGAGAGGAGGVGGAGGLLGA 233 G G G G GGA GW GG G G GG G G GG + Sbjct: 12 GAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNGNS 71 Query: 234 GGHGGAGG 241 GG G GG Sbjct: 72 GGGSGTGG 79 Score = 31.6 bits (71), Expect = 0.015 Identities = 36/120 (30%), Positives = 45/120 (37%), Gaps = 7/120 (5%) Query: 148 GNGGAGGSAAAGSGLPGGAGGAAGLFGTGGAGGAGGSST---VGDGEAGGAGGSGGWLLG 204 G G G+ + + GG G G G + G+G SS G G G GG G Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGV--GGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHG 63 Query: 205 TGGVGGVGGLGAGAGGAGGVGGAGGLLG--AGGHGGAGGLGAVTGGVGGTGGAGGLLAGL 262 GG G G G+G GG A G A GAGGL + ++A L Sbjct: 64 NGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAAIADIMAAL 123 Score = 31.2 bits (70), Expect = 0.017 Identities = 30/91 (32%), Positives = 35/91 (38%), Gaps = 6/91 (6%) Query: 567 LGGTGGGGGVGGLWGAGGAGGAGGTGLVGGDGGAGGAGGT------GGLLAGLIGAGGGH 620 + G G G G G G TGL G G + G+G + GG I GGG Sbjct: 1 MSGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGS 60 Query: 621 GGTGGLSTNGDGGVGGAGGNAGMLAGPGGAG 651 G G GG G GGN +A P G Sbjct: 61 GHGNGGGNGNSGGGSGTGGNLSAVAAPVAFG 91 Score = 31.2 bits (70), Expect = 0.018 Identities = 31/108 (28%), Positives = 38/108 (35%), Gaps = 7/108 (6%) Query: 678 SGGAGGAGGFGFLGGDGGAGGNAGLLLSSGGAGGFGGFGTAGGVGGAGGNAGWLGFGGAG 737 SGG G G G G L GGA G+ + G G +G GG+G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 738 GVGGSAGLIGTGGNGGNGGTGANAGSPGTG-------GAGGLLLGQNG 778 G GG+G G A A G GAGGL + + Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISA 109 Score = 30.8 bits (69), Expect = 0.025 Identities = 34/106 (32%), Positives = 41/106 (38%), Gaps = 5/106 (4%) Query: 498 GDGGAGGSGAAGSGAPGGAGGAAGLWGTGGAGGAGGSSAGGGGAGGAGGAGGWLLGDGGA 557 GDG +GA + G G G + G+G SS GG+G W G G Sbjct: 4 GDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHG 63 Query: 558 GGIGGASTVLGGTGGGGGVGGLWGAGGAGGAGGTGLVGGDGGAGGA 603 G G G +GGG G GG A A A G + G G A Sbjct: 64 NGGGN-----GNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 30.5 bits (68), Expect = 0.032 Identities = 32/110 (29%), Positives = 39/110 (35%), Gaps = 1/110 (0%) Query: 205 TGGVGGVGGLGAGAGGAGGVGGAGGLLGAGGHGGAGGLGAVTGGVGGTGGAG-GLLAGLL 263 +GG G GA + GG GL GG G + GG G+G G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 264 AGPGGAGGTGGRGFLNNGGVGGAGGNAGLLFGAGGTGGSGGAGLGGDGGA 313 G GG G G G G + F A T G+GG + GA Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGA 111 Score = 30.1 bits (67), Expect = 0.035 Identities = 30/96 (31%), Positives = 40/96 (41%), Gaps = 2/96 (2%) Query: 358 GGAGGFGETGDGGVGGA--GGKAGLLIGNGGAGGAGGQGAVTGGTGGAGGDGVLIGNGGN 415 GG G TG G GG GL +G G + G+G GG+G G G+ Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 416 AGIGGTGPTAGDTGAGGISGLLLGADGFNTPASASP 451 GG G + G +G GG + F PA ++P Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTP 98 Score = 30.1 bits (67), Expect = 0.040 Identities = 25/81 (30%), Positives = 29/81 (35%) Query: 690 LGGDGGAGGNAGLLLSSGGAGGFGGFGTAGGVGGAGGNAGWLGFGGAGGVGGSAGLIGTG 749 + G G G N G +SG G GG G GG G G Sbjct: 1 MSGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGS 60 Query: 750 GNGGNGGTGANAGSPGTGGAG 770 G+G GG G + G GTGG Sbjct: 61 GHGNGGGNGNSGGGSGTGGNL 81 Score = 30.1 bits (67), Expect = 0.042 Identities = 32/110 (29%), Positives = 42/110 (38%), Gaps = 7/110 (6%) Query: 353 GSGGVGGAGGFGETGDGGVGGAGGKAGLLIGNGGAGGAGGQGAVTGGTGGAGGDGVLIGN 412 G+ G G TG G GGA +G N GG G G GG G G N Sbjct: 12 GAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHG-------N 64 Query: 413 GGNAGIGGTGPTAGDTGAGGISGLLLGADGFNTPASASPLHTLKQQALAA 462 GG G G G G + + + G +TP + ++ AL+A Sbjct: 65 GGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSA 114
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv0747	cloacin	38	1e-04	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 38.2 bits (88), Expect = 1e-04 Identities = 38/120 (31%), Positives = 48/120 (40%) Query: 531 GNGGAGGNGGLFANGGAGGPGGFGSPAGAGGIGGAGGNGGLFGAGGTGGAGGGSTLAGGA 590 G G G N G + G G G G G G+G + GG G+G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 591 GGAGGNGGLFGAGGTGGAGSHSTAAGVSGGAGGAGGDAGLLSLGASGGAGGSGGSSLTAA 650 G GGNG G GTGG S A G + AG L++ S GA + + + AA Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAAIADIMAA 122 Score = 34.7 bits (79), Expect = 0.002 Identities = 33/91 (36%), Positives = 42/91 (46%), Gaps = 6/91 (6%) Query: 638 GAGGSGGSSLTAAGVVGGIGGAGGLLFGSGGAGGSGGFS--NSGNGGAGGAGGDAGLLVG 695 G G + G+ T+ + GG G G GGA G+S N+ GG G+G G G Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGV----GGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 696 SGGAGGAGASATGAATGGDGGAGGKSGAFGL 726 G GG G S G+ TGG+ A AFG Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAAPVAFGF 92 Score = 34.3 bits (78), Expect = 0.002 Identities = 31/106 (29%), Positives = 45/106 (42%), Gaps = 5/106 (4%) Query: 544 NGGAGGPGGFGSPAGAGGIGGAGGNGGLFGAGGTGGAGGGSTLAGGAGGAGGNGGLFGAG 603 +GG G G+ + +G I G G+ G G + G+G S GG+G G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGV-GGGASDGSGWSSENNPWGGGSGSGIHWGGGS 60 Query: 604 GTGGAGSHSTAAGVSGGAGGAGGDAGLLSLG----ASGGAGGSGGS 645 G G G + + G SG G A ++ G ++ GAGG S Sbjct: 61 GHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVS 106 Score = 33.9 bits (77), Expect = 0.003 Identities = 36/113 (31%), Positives = 44/113 (38%), Gaps = 4/113 (3%) Query: 197 GGVGGFSNGGA--TGGAGGAGGAGGLFGAGRERGSGGSGNLTGGAGGAGGNAGTLATGDG 254 GG G N GA T G G G G G GSG S GG+G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 255 GAGGTGGASRSGGFGGAGGAGGDAGMFFG--SGGSGGAGGISKSVGDSAAGGA 305 G GG G S G G + A + FG + + GAGG++ S+ A A Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAA 115 Score = 33.5 bits (76), Expect = 0.003 Identities = 33/93 (35%), Positives = 39/93 (41%), Gaps = 8/93 (8%) Query: 430 GNGGNGG-QGTIGGVNGGAGGAGGAGGILFGTGGTGGSGGPGATGLGGIGGAGGAALLFG 488 G G N G T G +NGG G G GG G+G + + G GG+G G Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWG-------GGSGSGIHWGG 58 Query: 489 SGGAGGSGGAGAVGGNGGAGGNAGALLGAAGAG 521 G G GG G GG G GGN A+ G Sbjct: 59 GSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFG 91 Score = 33.5 bits (76), Expect = 0.003 Identities = 39/120 (32%), Positives = 48/120 (40%), Gaps = 11/120 (9%) Query: 459 GTGGTGGSGGPGATGLGGIGGAGGAALLFGSGGAGGSGGAGAVGGNGGAGGNAGALLGAA 518 G G G + G +T GG G G G S G+G N GG +G+ + Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTG-----LGVGGGASDGSGWSSENNPWGGGSGSGIHWG 57 Query: 519 GAGGAGGAGAVGGNGGAGGNGGLFANGGAGGPGGFGSPA----GAGGIGGAGGNGGLFGA 574 G G G G G +GG G GG P FG PA GAGG+ + G L A Sbjct: 58 GGSGHGNGGGNGNSGGGSGTGG--NLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAA 115 Score = 33.1 bits (75), Expect = 0.004 Identities = 27/101 (26%), Positives = 37/101 (36%) Query: 283 GSGGSGGAGGISKSVGDSAAGGAGGAPGLIGNGGNGGNGGASTGGGDGGPGGAGGTGVLI 342 G G G G + G+ G G G + G+G + + GG G G G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 343 GNGGNGGSGGTGATLGKAGIGGTGGVLLGLDGFTAPASTSP 383 GNGG G+ G G+ G V G + P + Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGL 103 Score = 32.4 bits (73), Expect = 0.009 Identities = 28/95 (29%), Positives = 32/95 (33%) Query: 117 NGANGAPGTGANGGPGGWLIGNGGAGGSGAPGAGAGGNGGAGGLFGSGGAGGASTDVAGG 176 N + NGGP G +G G + GSG GG+G GG G G Sbjct: 10 NTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNG 69 Query: 177 AGGAGGAGGNAGMLFGAAGVGGVGGFSNGGATGGA 211 G G G A G S GA G A Sbjct: 70 NSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 32.0 bits (72), Expect = 0.009 Identities = 28/85 (32%), Positives = 31/85 (36%), Gaps = 2/85 (2%) Query: 503 GNGGAGGNAGALLGAAGAGGAGGAGAVGGNGGAGGNGGLFANGGAGGPGGFGSPAGAGGI 562 G G G N GA + G VGG G N GG G G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 563 GGAGGNGGLFGAGGTGGAGGGSTLA 587 G GGNG GG+G G S +A Sbjct: 63 GNGGGNGN--SGGGSGTGGNLSAVA 85 Score = 31.6 bits (71), Expect = 0.013 Identities = 30/84 (35%), Positives = 34/84 (40%), Gaps = 6/84 (7%) Query: 229 SGGSGNLTGGAGGAGGNAGTLATGDGGAGGTGGASRSGGFGGA----GGAGGDAGMFFGS 284 SGG G G GA +G + G G G GGAS G+ GG G + G Sbjct: 2 SGGDGR--GHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGG 59 Query: 285 GGSGGAGGISKSVGDSAAGGAGGA 308 G G GG S G S GG A Sbjct: 60 SGHGNGGGNGNSGGGSGTGGNLSA 83 Score = 31.2 bits (70), Expect = 0.018 Identities = 33/100 (33%), Positives = 38/100 (38%), Gaps = 5/100 (5%) Query: 488 GSGGAGGSGGAGAVGGN-----GGAGGNAGALLGAAGAGGAGGAGAVGGNGGAGGNGGLF 542 G G G + GA + GN G G GA G+ + G G+G G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 543 ANGGAGGPGGFGSPAGAGGIGGAGGNGGLFGAGGTGGAGG 582 NGG G G GS G A F A T GAGG Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG 102 Score = 31.2 bits (70), Expect = 0.018 Identities = 26/71 (36%), Positives = 34/71 (47%), Gaps = 2/71 (2%) Query: 669 AGGSGGFSNSGNGGAGGA--GGDAGLLVGSGGAGGAGASATGAATGGDGGAGGKSGAFGL 726 +GG G N+G G GG GL VG G + G+G S+ GG G+G G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 727 GGDGGAGGATG 737 G+GG G +G Sbjct: 62 HGNGGGNGNSG 72 Score = 31.2 bits (70), Expect = 0.021 Identities = 35/112 (31%), Positives = 47/112 (41%), Gaps = 13/112 (11%) Query: 601 GAGGTGGAGSHSTAAGVSGGAGGAGGDAGLLSLGASGGAGGSGGSSLTAAGVVGGIGGAG 660 G G G+HST+ ++GG G G G G S GS ++ G G Sbjct: 4 GDGRGHNTGAHSTSGNINGGPTGLGV-----------GGGASDGSGWSSENNPWGGGSGS 52 Query: 661 GLLFGSGGAGGSGGFSNSGNGGAGGAGGDAGLLVGSGGAGGAGASATGAATG 712 G+ +G G G+GG +GN G G G V + A G A +T A G Sbjct: 53 GIHWGGGSGHGNGG--GNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG 102 Score = 30.8 bits (69), Expect = 0.024 Identities = 25/86 (29%), Positives = 33/86 (38%) Query: 228 GSGGSGNLTGGAGGAGGNAGTLATGDGGAGGTGGASRSGGFGGAGGAGGDAGMFFGSGGS 287 G G + +G G L G G + G+G +S + +GG G+G G G G Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNG 65 Query: 288 GGAGGISKSVGDSAAGGAGGAPGLIG 313 GG G G A AP G Sbjct: 66 GGNGNSGGGSGTGGNLSAVAAPVAFG 91 Score = 30.5 bits (68), Expect = 0.030 Identities = 25/80 (31%), Positives = 36/80 (45%), Gaps = 1/80 (1%) Query: 156 GAGGLFGSGGAGGASTDVAGGAGGAGGAGG-NAGMLFGAAGVGGVGGFSNGGATGGAGGA 214 G G + GA S ++ GG G G GG + G + + GG +G GG G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 215 GGAGGLFGAGRERGSGGSGN 234 G GG +G G+GG+ + Sbjct: 63 GNGGGNGNSGGGSGTGGNLS 82 Score = 30.5 bits (68), Expect = 0.035 Identities = 26/95 (27%), Positives = 34/95 (35%), Gaps = 3/95 (3%) Query: 562 IGGAGGNGGLFGAGGTGGAGGGSTLAGGAGGAGGNGGLFGAGGTGGAGSHSTAAGVSGGA 621 + G G G GA T G G G GG +G + + G + GG+ Sbjct: 1 MSGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGS 60 Query: 622 GGAGGDAGLLSLGASGGAGGSGGSSLTAAGVVGGI 656 G G + GG+G G S AA V G Sbjct: 61 GHGNGGGN---GNSGGGSGTGGNLSAVAAPVAFGF 92 Score = 30.1 bits (67), Expect = 0.036 Identities = 32/107 (29%), Positives = 38/107 (35%), Gaps = 6/107 (5%) Query: 137 GNGGAGGSGAPGAGAGGNGGAGGLFGSGGAGGASTDVAGGAGGAGGAGGNAGMLFGAAGV 196 G+G +GA NGG GL GGA S + GG+G Sbjct: 4 GDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWG------ 57 Query: 197 GGVGGFSNGGATGGAGGAGGAGGLFGAGRERGSGGSGNLTGGAGGAG 243 GG G + GG GG+G G L G T GAGG Sbjct: 58 GGSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 30.1 bits (67), Expect = 0.042 Identities = 28/107 (26%), Positives = 43/107 (40%), Gaps = 6/107 (5%) Query: 577 TGGAGGGSTLAGGAGGAGGNGGLFGAGGTGGAGSHSTAAGVSGGAGGAGGDAGLLSLGAS 636 TG + GG G G GG + G+G + ++ G SG GG +G + Sbjct: 11 TGAHSTSGNINGGPTGLGVGGG--ASDGSGWSSENNPWGGGSGSGIHWGGGSG----HGN 64 Query: 637 GGAGGSGGSSLTAAGVVGGIGGAGGLLFGSGGAGGSGGFSNSGNGGA 683 GG G+ G G + + F + G+GG + S + GA Sbjct: 65 GGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGA 111 Score = 29.7 bits (66), Expect = 0.048 Identities = 20/56 (35%), Positives = 27/56 (48%) Query: 115 IGNGANGAPGTGANGGPGGWLIGNGGAGGSGAPGAGAGGNGGAGGLFGSGGAGGAS 170 +G GA+ G + P G G+G G G+ GGNG +GG G+GG A Sbjct: 29 VGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNGNSGGGSGTGGNLSAV 84
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv0748	PF06057	26	0.028	Type IV secretory pathway VirJ component
>PF06057#Type IV secretory pathway VirJ component Length = 243 Score = 25.6 bits (56), Expect = 0.028 Identities = 12/61 (19%), Positives = 20/61 (32%) Query: 11 ILAAAKRRARERGQSLGAVIEDALRREFAAAHVGGARPTVPVFDGGTGPRRGIDLTSNRA 70 + + A A E +LG + A +P + +F G G +D Sbjct: 14 LCSTANAFADEFADNLGLTLLPVEPSTQVNAASSHTKPPLVIFLSGDGGWATLDKAVGGI 73 Query: 71 L 71 L Sbjct: 74 L 74
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv0754	cloacin	42	6e-06	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 42.0 bits (98), Expect = 6e-06 Identities = 34/110 (30%), Positives = 42/110 (38%), Gaps = 6/110 (5%) Query: 175 GDGGAGYTGGNGGSAGLIGNGGTGGAGFAGGVGGMGGTGGWLMGNGGMGGAG-GVGGNGG 233 G G G+ G ++G I NGG G G GG G GG G+G GG G Sbjct: 3 GGDGRGHNTGAHSTSGNI-NGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 234 AGGQALLFGNGGLGGAGGAGGVDGAIGRGGWFIGTGGMATIGGGGNGQSI 283 G G G G G G + + G ++T G GG SI Sbjct: 62 HGNG----GGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSI 107 Score = 41.6 bits (97), Expect = 8e-06 Identities = 36/98 (36%), Positives = 40/98 (40%), Gaps = 8/98 (8%) Query: 157 GVTGTAAAPNGGPGGLLFGDGGAGYTGGNGGSAGLIGNGGTG-GAGFAGGVGGMGGTGGW 215 G T+ NGGP GL G GG G S GG+G G + GG G G Sbjct: 12 GAHSTSGNINGGPTGL--GVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGG--- 66 Query: 216 LMGNGGMGGAGGVGGNGGAGGQALLFGNGGLGGAGGAG 253 GNG GG G GGN A + FG L G G Sbjct: 67 --GNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG 102 Score = 37.4 bits (86), Expect = 1e-04 Identities = 39/137 (28%), Positives = 56/137 (40%), Gaps = 13/137 (9%) Query: 197 TGGAGFAGGVGGMGGTGGWLMGNGGMGGAGGVGGNGGAGGQALLFGNGGLGGAGGAGGVD 256 +GG G G G T G + G G GG G + G+G + GG G+G G Sbjct: 2 SGGDG-RGHNTGAHSTSGNINGGPTGLGVGG-GASDGSGWSSENNPWGGGSGSGIHWGGG 59 Query: 257 GAIGRGGWFIGTGGMATIGGGGNGQSIVIDF-VRHGQTPGNAAMLIDTAVPGPGLTALGQ 315 G GG +GG + GG + + + F TPG G ++ Sbjct: 60 SGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAG---------GLAVSISAG 110 Query: 316 QQAQAIANALAA-KGPY 331 + AIA+ +AA KGP+ Sbjct: 111 ALSAAIADIMAALKGPF 127
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv0755c	cloacin	35	0.001	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 35.1 bits (80), Expect = 0.001 Identities = 28/93 (30%), Positives = 32/93 (34%), Gaps = 2/93 (2%) Query: 253 SGNIGNANVGGGNSGDNNFGFGNFGNANIGIGNAGPNMSSPAVPTPGNGNVGIGNGGNGN 312 SG G + G +S N G G G + G SS P G GI GG Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGS- 60 Query: 313 FGGGNTGNANIGLGNVGDGNVGFGNSGSYNFGF 345 G GN G G G G + FGF Sbjct: 61 -GHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGF 92 Score = 32.8 bits (74), Expect = 0.004 Identities = 27/75 (36%), Positives = 36/75 (48%), Gaps = 1/75 (1%) Query: 310 NGNFGGGNTGNANIGLGNVGDGNVGFGNSGSYNFGFGNTGNNNIGIGLTGSNQIGFGGLN 369 +G G G+ A+ GN+ G G G G + G G + NN G +GS GG Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 370 SGSGNIGFGNSGTGN 384 G+G G GNSG G+ Sbjct: 62 HGNGG-GNGNSGGGS 75 Score = 30.1 bits (67), Expect = 0.033 Identities = 27/92 (29%), Positives = 37/92 (40%), Gaps = 15/92 (16%) Query: 226 GSGNIGNFNLGSGNGNVGIGPSSFNVGSGNIGNANVGGGNSGDNNFGFGNFGNANIGIGN 285 G G N S +GN+ GP+ VG GG + G G+ + N G Sbjct: 4 GDGRGHNTGAHSTSGNINGGPTGLGVG---------GGASDGS---GWSSENNPWGGGSG 51 Query: 286 AGPNMSSPAVPTPGNGNVGIGNGGNGNFGGGN 317 +G + + G GN GN G G+ GGN Sbjct: 52 SGIHWGGGSGHGNGGGN---GNSGGGSGTGGN 80

Rv0828c

Rv0834c

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
Rv0828c	7	20	5.769523	Possible deaminase
Rv0829	9	20	8.167830	Possible transposase (fragment)
Rv0830	8	20	7.449210	Possible S-adenosylmethionine-dependent
Rv0831c	8	20	8.241251	Conserved protein
Rv0832	6	19	8.504112	****PE-PGRS family protein PE_PGRS12
Rv0833	5	18	8.008832	PE-PGRS family protein PE_PGRS13
Rv0834c	0	15	4.488468	PE-PGRS family protein PE_PGRS14

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv0832	RTXTOXINA	27	0.027	Gram-negative bacterial RTX toxin determinant A family...
>RTXTOXINA#Gram-negative bacterial RTX toxin determinant A family signature. Length = 1024 Score = 27.2 bits (60), Expect = 0.027 Identities = 26/107 (24%), Positives = 39/107 (36%), Gaps = 13/107 (12%) Query: 20 RIGSALSLASAVAAAQTSAVQAAAADEVSAAIAALFSAHGRDFQALSARA---------- 69 R LS ++A A SAV A + +IA F + S R Sbjct: 295 RAAQGLSTSAAAAGLIASAVTLAISPLSFLSIADKFK-RANKIEEYSQRFKKLGYDGDSL 353 Query: 70 -AAFHHEFVQALAAGAG-SYAVAEIAAASPLQSLIDVFNAPIQAATG 114 AAFH E A+ S +A +++ + + AP+ A G Sbjct: 354 LAAFHKETGAIDASLTTISTVLASVSSGISAAATTSLVGAPVSALVG 400
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv0833	cloacin	39	5e-05	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 39.3 bits (91), Expect = 5e-05 Identities = 36/126 (28%), Positives = 47/126 (37%) Query: 273 AGGSGGAGGFANGSTGGAGGAGGGAGLIGNGGNGGSGGTSVATGGAGNGGAGGAGGGAGL 332 +GG G ST G G +G G + GSG +S G G+G GG Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 333 IGNGGNGGSGGMGDAPGGTGVGGIGGLLLGLDGANAPASTNPLHTAQQQALAAVNAPIQA 392 GNGG G+ G G GG + G + P + + AL+A A I A Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAAIADIMA 121 Query: 393 VTGRPL 398 P Sbjct: 122 ALKGPF 127 Score = 37.4 bits (86), Expect = 2e-04 Identities = 35/107 (32%), Positives = 43/107 (40%), Gaps = 5/107 (4%) Query: 512 GAGGAGGSGVSGGAGGEGGAGGAGGLFAGGGAGGAGGSGNNVGGAGGAGGVGGLFGAGGA 571 G G G + + G G G GG + G+G S N GG+G G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 572 GGSGGGGSVAGDSGAGGNAGLLAPGLAGGAGGGGGQGFDTGGAGGPG 618 G GG G+ G SG GGN +A +A G T GAGG Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFGF-----PALSTPGAGGLA 104 Score = 35.8 bits (82), Expect = 6e-04 Identities = 34/119 (28%), Positives = 45/119 (37%), Gaps = 1/119 (0%) Query: 477 GAGGAGGAGGSSGIGGFAAGGAGGPGGAGGLFNGGGAGGAGGSGVSGGAGGEGGAGGAGG 536 G G G G+ G GG G G GG +G G G G GG+G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 537 LFAGGGAGGAGGSGNNVGGAGGAGGVGGLFGAGGAGGSGGGG-SVAGDSGAGGNAGLLA 594 GG GGSG + A V F A G+GG S++ + + A ++A Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAAIADIMA 121 Score = 35.5 bits (81), Expect = 9e-04 Identities = 41/120 (34%), Positives = 53/120 (44%), Gaps = 7/120 (5%) Query: 1 MIGNGGAGGSGAPGAIGGA--GGPAGLIGVGGAGGAGGDSAVAGVIGGAGGAGGAALLFG 58 M G G G + + G GGP GL GGA G S+ GG G+G Sbjct: 1 MSGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSG-----IH 55 Query: 59 AGGAGGAGGSGGSGAAGGAGGAGGAGGLFASGGSGGFGGFASTGTGGAGGTGGAGGLFAS 118 GG G G GG+G +GG G GG A+ + GF ++ G GG + AG L A+ Sbjct: 56 WGGGSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAA 115 Score = 34.3 bits (78), Expect = 0.002 Identities = 27/82 (32%), Positives = 34/82 (41%), Gaps = 1/82 (1%) Query: 61 GAGGAGGSGGSGAAGGAGGAGGAGGLFASGGSGGFGGFASTGTGGAGGTGGAGGLFASGG 120 G G G + G+ + G GG GL GG+ G++S GG+G G Sbjct: 3 GGDGRGHNTGAHSTSGNIN-GGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 121 VGGTGGGAGSGGTGGVGGTGGA 142 G GG SGG G GG A Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSA 83 Score = 34.3 bits (78), Expect = 0.002 Identities = 39/107 (36%), Positives = 45/107 (42%), Gaps = 7/107 (6%) Query: 497 GAGGPGGAGGLFNGGGA--GGAGGSGVSGGAGGEGGAGGAGGLFAGGGAGGAGGSGNNVG 554 G G G G + G GG G GV GGA + G+G GG GSG + G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGA-----SDGSGWSSENNPWGGGSGSGIHWG 57 Query: 555 GAGGAGGVGGLFGAGGAGGSGGGGSVAGDSGAGGNAGLLAPGLAGGA 601 G G G GG +GG G+GG S A G L PG G A Sbjct: 58 GGSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 33.9 bits (77), Expect = 0.002 Identities = 36/114 (31%), Positives = 46/114 (40%), Gaps = 4/114 (3%) Query: 109 TGGAGGLFASGGVGGTGGGAGSGGTGGVGGTGGAGGLFASGGAGGAGGSGGTGGAGGTGG 168 +GG G +G +G G GVGG G ++S GGSG GG G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 169 AGGLFGAGGAGGLGGQGNHTGGHGGAGGSAGLLALGDGGAGGAGGAATTGTGGA 222 G GG G G G+ TGG+ A + GAGG A + + GA Sbjct: 62 HGN----GGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGA 111 Score = 33.5 bits (76), Expect = 0.003 Identities = 38/121 (31%), Positives = 48/121 (39%), Gaps = 9/121 (7%) Query: 120 GVGGTGGGAGSGGTGGVGGTGGAGGLFASGGAGGAGGSGGTGGAGGTGGAGGLFGAGGAG 179 G G G G+ T G G G G + G+G S GG G+G +G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 180 GLGGQGNHTGGHGGAGGSA---------GLLALGDGGAGGAGGAATTGTGGAGGAGGKAG 230 G GG ++GG G GG+ G AL GAGG + + G A A A Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAAIADIMAA 122 Query: 231 L 231 L Sbjct: 123 L 123 Score = 33.1 bits (75), Expect = 0.004 Identities = 26/82 (31%), Positives = 33/82 (40%) Query: 656 SGGAGGAGGSGRTDLGGAGGAGGKAGLIGNGGNGGAGGAGGNGGGDGGPGGAAFGLGNGG 715 SGG G +G G G +G G + G+G + N GG G G G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 716 NGGNGGTGTSAGSPGAGGAGGS 737 +G GG G S G G GG + Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSA 83 Score = 32.8 bits (74), Expect = 0.006 Identities = 35/115 (30%), Positives = 46/115 (40%), Gaps = 1/115 (0%) Query: 627 SGGVGGAGGFGLTTGGPGAAGGDAGLLFGSGGAGGAGGSGRTDLGGAGGAGGKAGLIGNG 686 SGG G G + GG GL G G + G+G S + G GG+G G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWG-GGSGSGIHWGGGS 60 Query: 687 GNGGAGGAGGNGGGDGGPGGAAFGLGNGGNGGNGGTGTSAGSPGAGGAGGSLIGA 741 G+G GG G +GGG G G + G + AG + G+L A Sbjct: 61 GHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAA 115 Score = 32.4 bits (73), Expect = 0.007 Identities = 37/114 (32%), Positives = 44/114 (38%), Gaps = 7/114 (6%) Query: 425 TGGSGVSGGAGGDGGAGGILFGAGGAGGAGGAVTGTG----ATGGSGGAGGGALLFGAGG 480 +GG G G +G I G G G GGA G+G GG+G G G G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 481 AGGAGGSSGIGGFAAGGAGGPGGAGGL---FNGGGAGGAGGSGVSGGAGGEGGA 531 G GG+ GG + G A + F GAGG VS AG A Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAA 115 Score = 32.4 bits (73), Expect = 0.008 Identities = 26/82 (31%), Positives = 38/82 (46%) Query: 245 AGTFGDTGNSGGAGGAGGKAGLLFGSGGAGGSGGAGGFANGSTGGAGGAGGGAGLIGNGG 304 +G G N+G +G G G G GG+ G+++ + GG+G G G G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 305 NGGSGGTSVATGGAGNGGAGGA 326 +G GG + GG+G GG A Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSA 83 Score = 32.4 bits (73), Expect = 0.008 Identities = 31/116 (26%), Positives = 44/116 (37%), Gaps = 4/116 (3%) Query: 60 GGAGGAGGSGGSGAAGGAGGAGGAGGLFASGGSGGFGGFASTGTGGAGGTGGAGGLFASG 119 GA G+ G G G G + G S + +GG + +G GG+G G G Sbjct: 11 TGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNG----G 66 Query: 120 GVGGTGGGAGSGGTGGVGGTGGAGGLFASGGAGGAGGSGGTGGAGGTGGAGGLFGA 175 G G +GGG+G+GG A G A G G + + + A Sbjct: 67 GNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAAIADIMAA 122 Score = 31.6 bits (71), Expect = 0.014 Identities = 34/111 (30%), Positives = 50/111 (45%), Gaps = 2/111 (1%) Query: 180 GLGGQGNHTGGHGGAGGSAGLLALGDGGAGGAGGAATTGTGGAGGAGGKAGLLFGSGGAG 239 G G+G++TG H +G G G G + G+ + G G +G+ +G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 240 GSGGAAGTFGDTGNSGGAGGAGGKAGLLFGSGGAGGSGGAGGFANGSTGGA 290 G+GG G G G+ G + A + FG A + GAGG A + GA Sbjct: 63 GNGGGNGNSGG-GSGTGGNLSAVAAPVAFGF-PALSTPGAGGLAVSISAGA 111 Score = 31.2 bits (70), Expect = 0.018 Identities = 36/120 (30%), Positives = 47/120 (39%), Gaps = 3/120 (2%) Query: 451 GGAGGAVTGTGATGGSGGAGGGALLFGAGGAGGAGGSSGIGGFAAGGAGGPGGAGGLFNG 510 G G TG +T G+ G L G G + G+G SS + G G GG +G Sbjct: 4 GDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHG 63 Query: 511 GGAGGAGGSGVSGGAGGEGGAGGAGGLFA--GGGAGGAGGSGNNVGGAGGAGGVGGLFGA 568 G GG G SG G GG A A F GAGG ++ + + + A Sbjct: 64 NG-GGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAAIADIMAA 122 Score = 30.5 bits (68), Expect = 0.029 Identities = 38/119 (31%), Positives = 44/119 (36%), Gaps = 5/119 (4%) Query: 80 AGGAGGLFASGGSGGFGGFASTGTGGAGGTGGAGGLFASGGVGGTGGGAGSGGTGGVGGT 139 +GG G +G G TG G G + G S GGG+GSG G G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 140 GGAGGLFASGGAGGAGGSGGTGGAGGTGGAGGLFGAGGAGGLGGQGNHTGGHGGAGGSA 198 G GG G G +GG GTGG A FG G G GA +A Sbjct: 62 HGNGG-----GNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAA 115 Score = 30.5 bits (68), Expect = 0.030 Identities = 30/102 (29%), Positives = 36/102 (35%), Gaps = 7/102 (6%) Query: 401 NGANGAPGSGAPGGHGGWLFGGGGTGGSGVSGGAGGDGGAGGILFGAGGAGGAGGAVTGT 460 N + GG G GGG + GSG S GG G GG G G Sbjct: 10 NTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNG 69 Query: 461 GATGGSGGAGGGALLFGAGGAGGAGGSSGIGGFAAGGAGGPG 502 + GGSG G + A A + G + GAGG Sbjct: 70 NSGGGSGTGGNLS-------AVAAPVAFGFPALSTPGAGGLA 104 Score = 30.1 bits (67), Expect = 0.036 Identities = 30/108 (27%), Positives = 39/108 (36%), Gaps = 5/108 (4%) Query: 414 GHGGWLFGGGGTGGSGVSGGAGGDGGAGGILFGAGGAGGAGGAVTGTGATGGSGGAGGGA 473 GH G G +G G G + G + + GG+ +G GGSG GG Sbjct: 8 GHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGG 67 Query: 474 LLFGAGGAGGAGGSSGIG-----GFAAGGAGGPGGAGGLFNGGGAGGA 516 GG+G G S + GF A G GG + G A Sbjct: 68 NGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAA 115
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv0834c	cloacin	39	1e-04	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 38.5 bits (89), Expect = 1e-04 Identities = 37/98 (37%), Positives = 42/98 (42%), Gaps = 1/98 (1%) Query: 498 GGNGGAGGSGTGLLGGVGGAGGHGGGASVGTGGSGGAGGDGFGFVGAGGNGGNAGTGVGV 557 G N GA + + GG G G GGGAS G+G S G G GG +G G G Sbjct: 8 GHNTGAHSTSGNINGGPTGLGV-GGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGG 66 Query: 558 NGANGGNGGSATGALAAVGGAGAAGGDATSGTGGFGGA 595 N G G G L+AV A G A S G G A Sbjct: 67 GNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 38.2 bits (88), Expect = 1e-04 Identities = 35/114 (30%), Positives = 40/114 (35%), Gaps = 4/114 (3%) Query: 256 AGGAATTGTGGAGGAGSNALGLFLGLGGSGGQGGDSAMGSGGAGGAGGSGGAASPFGIDI 315 +GG GA N G GLG GG S S GGSG G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGG-- 59 Query: 316 GIGGAGGHGGAGTNGGAGGAGGAGGSSGTVFALDLSWGGAGGNGGAATTGTGGA 369 G G GG G +GG G GG + A G GG A + + GA Sbjct: 60 --SGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGA 111 Score = 36.6 bits (84), Expect = 4e-04 Identities = 44/122 (36%), Positives = 50/122 (40%), Gaps = 17/122 (13%) Query: 150 GSGAPGQTGGAGGAAGLLGHGGTGGAGGTGASGGKGGTGGWLWGSGGAGGAGGSGGGSGG 209 G G GA +G + G TG G GAS G G W S GGSG G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSG------WSSENNPWGGGSGSGIHW 56 Query: 210 AGGNALMFGIGGNGGAGGAASGVGNGGVGGAGGAGGALVAIG----GAGGAGGAATTGTG 265 GG+ G G G SG G+G GG A A VA G GAGG A + + Sbjct: 57 GGGS------GHGNGGGNGNSGGGSGT-GGNLSAVAAPVAFGFPALSTPGAGGLAVSISA 109 Query: 266 GA 267 GA Sbjct: 110 GA 111 Score = 36.2 bits (83), Expect = 7e-04 Identities = 37/125 (29%), Positives = 49/125 (39%), Gaps = 16/125 (12%) Query: 174 GAGGTGASGGKGGTGGWLWGSGGAGGAGGSGGGSGGAGGNALMFGIGGNGGAGGAASGVG 233 G G G + G T G + +GG G G GG S G+G + N GG+ SG+ Sbjct: 3 GGDGRGHNTGAHSTSGNI--NGGPTGLGVGGGASDGSG-----WSSENNPWGGGSGSGIH 55 Query: 234 NGGVGGAGGAGGALVAIGGAGGAGGAATTGTGGAGGAGSNALGLFLGLGGSGGQGGDSAM 293 GG G G GG +GTGG A + + + G GG + Sbjct: 56 WGGGSGHGNGGG---------NGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVS 106 Query: 294 GSGGA 298 S GA Sbjct: 107 ISAGA 111 Score = 34.7 bits (79), Expect = 0.002 Identities = 35/104 (33%), Positives = 40/104 (38%), Gaps = 7/104 (6%) Query: 353 GGAGGNGGAATTGTGGAGGTGGFAVAPDFIGFGAAYGGAGGLGGAATGAGGTGGTGGVGA 412 GA G G G G GG A D G+ + GG G+ GG G G G Sbjct: 11 TGAHSTSGNINGGPTGLGVGGG---ASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGG 67 Query: 413 GGFAALGVGVGGAGGAGGAATETG----GIGGAGGLGVGLLGGA 452 G + G G GG A A G GAGGL V + GA Sbjct: 68 NGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGA 111 Score = 34.7 bits (79), Expect = 0.002 Identities = 32/120 (26%), Positives = 44/120 (36%), Gaps = 2/120 (1%) Query: 295 SGGAGGAGGSGGAASPFGIDIGIGGAGGHGGAGTNGGAGGAGG--AGGSSGTVFALDLSW 352 SGG G +G ++ I+ G G G GGA G GGS + S Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 353 GGAGGNGGAATTGTGGAGGTGGFAVAPDFIGFGAAYGGAGGLGGAATGAGGTGGTGGVGA 412 G GG G + G+G G A F + GAGGL + + + + A Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAAIADIMA 121 Score = 33.1 bits (75), Expect = 0.005 Identities = 32/112 (28%), Positives = 38/112 (33%), Gaps = 2/112 (1%) Query: 131 GTGEAGGPGGWLLGNGGNGGSGAPGQTGGAGGAAGLLGHGGTGGAGGTGASGGKGGTGGW 190 G G G NGG G GGA +G G G GG+G Sbjct: 4 GDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHG 63 Query: 191 LWGSGGAGGAGGSGGGSGGAGGNALMFGIGG--NGGAGGAASGVGNGGVGGA 240 G G G G GG+ A + FG GAGG A + G + A Sbjct: 64 NGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAA 115 Score = 32.8 bits (74), Expect = 0.007 Identities = 38/123 (30%), Positives = 46/123 (37%), Gaps = 8/123 (6%) Query: 216 MFGIGGNGGAGGAASGVGNGGVGGAGGAGGALVAIGGAGGAGGAATTGTGGAGGAGSNAL 275 M G G G GA S GN G G G GGA G ++ GG+GS Sbjct: 1 MSGGDGRGHNTGAHSTSGNINGGPTGLGVG-----GGASDGSGWSSENNPWGGGSGS--- 52 Query: 276 GLFLGLGGSGGQGGDSAMGSGGAGGAGGSGGAASPFGIDIGIGGAGGHGGAGTNGGAGGA 335 G+ G G G GG + GG+G G A+P G GG + AG Sbjct: 53 GIHWGGGSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGAL 112 Query: 336 GGA 338 A Sbjct: 113 SAA 115 Score = 32.8 bits (74), Expect = 0.007 Identities = 25/85 (29%), Positives = 34/85 (40%), Gaps = 5/85 (5%) Query: 449 LGGAGGAGGPGGAASAGSGGHGGTGGDALGLIGAGIGGVGGVGGAATDTGGNGGAGGSGT 508 + G G G GA S +GG G +G G G G G ++ + GG+G Sbjct: 1 MSGGDGRGHNTGAHSTSGNINGGPTG-----LGVGGGASDGSGWSSENNPWGGGSGSGIH 55 Query: 509 GLLGGVGGAGGHGGGASVGTGGSGG 533 G G GG G + G+G G Sbjct: 56 WGGGSGHGNGGGNGNSGGGSGTGGN 80 Score = 32.4 bits (73), Expect = 0.009 Identities = 30/95 (31%), Positives = 35/95 (36%), Gaps = 1/95 (1%) Query: 489 GVGGAATDTGGNGGAGGSGTGLLGGVGGAGGHGGGASVGTGGSG-GAGGDGFGFVGAGGN 547 G A T GN G +G G+ GG G + GGSG G G G GG Sbjct: 8 GHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGG 67 Query: 548 GGNAGTGVGVNGANGGNGGSATGALAAVGGAGAAG 582 GN+G G G G A+ GA G Sbjct: 68 NGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG 102 Score = 31.6 bits (71), Expect = 0.015 Identities = 33/114 (28%), Positives = 45/114 (39%), Gaps = 6/114 (5%) Query: 654 SGATGGAGGDGVFEGIAVLGLGFGGAAGAGGAATGDG-ATGGAGGFGGAGAGIANFLGFS 712 SG G G + G G GGA+ G G ++ GG+G+GI G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGG-- 59 Query: 713 VLHGGAGGAGGTATGTGGNGGAGGGGGLSSPVILGIGIGGAGGDGGGALGVLGG 766 G G GG GG+G G +++PV G G GG A+ + G Sbjct: 60 ---SGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAG 110 Score = 30.5 bits (68), Expect = 0.035 Identities = 36/120 (30%), Positives = 45/120 (37%), Gaps = 11/120 (9%) Query: 503 AGGSGTGLLGGVGGAGGHGGGASVGTGGSGGAGGDGFGFVGAGGNGGNAGTGVGVNGANG 562 +GG G G G G+ G G G GGA GG +G+G+ G +G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 563 GNGGSATGALAAVGGAGAAGGDATSGTGGFGGAGGSARGLIFALGGAGAAGGDASTGVGG 622 G GG G +GG SGTGG A + F AGG A + G Sbjct: 62 HGNG---------GGNGNSGG--GSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAG 110 Score = 30.5 bits (68), Expect = 0.037 Identities = 36/122 (29%), Positives = 45/122 (36%), Gaps = 10/122 (8%) Query: 543 GAGGNGGNAGTGVGVNGANGGNGGSATGALAAVGGAGAAGGDATSGTGGFGGAGGSARGL 602 G G N G T +NG G G VGG + G +S +GG GS Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLG---------VGGGASDGSGWSSENNPWGGGSGSGIHW 56 Query: 603 IFALGGAGAAGGDASTGVGGPGGPGGTGTASSPFGI-AIAIGGAGAQGGAGTSGATGGAG 661 G G S G G GG A FG A++ GAG + ++GA A Sbjct: 57 GGGSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAAI 116 Query: 662 GD 663 D Sbjct: 117 AD 118 Score = 30.1 bits (67), Expect = 0.040 Identities = 24/70 (34%), Positives = 30/70 (42%) Query: 402 GGTGGTGGVGAGGFAALGVGVGGAGGAGGAATETGGIGGAGGLGVGLLGGAGGAGGPGGA 461 G T G GG LGVG G + G+G ++ GG+G G G GG G Sbjct: 11 TGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNGN 70 Query: 462 ASAGSGGHGG 471 + GSG G Sbjct: 71 SGGGSGTGGN 80 Score = 30.1 bits (67), Expect = 0.043 Identities = 27/90 (30%), Positives = 35/90 (38%), Gaps = 1/90 (1%) Query: 194 SGGAGGAGGSGGGSGGAGGNALMFGIGGNGGAG-GAASGVGNGGVGGAGGAGGALVAIGG 252 SGG G +G S N G+G GGA G+ N GG G+G G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 253 AGGAGGAATTGTGGAGGAGSNALGLFLGLG 282 G GG +G G G +A+ + G Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAAPVAFG 91 Score = 30.1 bits (67), Expect = 0.049 Identities = 31/103 (30%), Positives = 39/103 (37%), Gaps = 4/103 (3%) Query: 753 AGGDGGGALGVLGGMGGDGGDGGEAVAVGIAVGGAGGAGGAAPTGNGGAGGNGGDALGLV 812 +GGDG G G G G +G+ G + G+G ++ G G G G Sbjct: 2 SGGDGRGHNT--GAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGG 59 Query: 813 GVGGNGGNAGTGFGANTGGNGGDT--TIVVNGMLAPSTLGYGG 853 GNGG G G + G V G A ST G GG Sbjct: 60 SGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG 102

Rv0864

Rv0878c

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
Rv0864	-2	16	3.245142	Probable molybdenum cofactor biosynthesis
Rv0865	-2	16	3.034645	Probable molybdopterin biosynthesis Mog protein
Rv0866	-1	15	3.210468	Probable molybdenum cofactor biosynthesis
Rv0867c	2	16	5.457323	Possible resuscitation-promoting factor RpfA
Rv0868c	2	11	2.387318	Probable molybdenum cofactor biosynthesis
Rv0869c	0	8	2.867527	Probable molybdenum cofactor biosynthesis
Rv0870c	1	8	2.392795	Possible conserved integral membrane protein
Rv0871	0	8	2.200195	Probable cold shock-like protein B CspB
Rv0872c	0	7	2.181736	PE-PGRS family protein PE_PGRS15
Rv0873	-1	9	-0.207278	Probable acyl-CoA dehydrogenase FadE10
Rv0874c	-1	11	0.804647	Conserved hypothetical protein
Rv0875c	2	12	-0.056258	Possible conserved exported protein
Rv0876c	0	14	0.114460	Possible conserved transmembrane protein
Rv0877	1	16	0.468091	Conserved hypothetical protein
Rv0878c	2	15	0.668359	PPE family protein PPE13

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv0865	ARGDEIMINASE	28	0.019	Bacterial arginine deiminase signature.
>ARGDEIMINASE#Bacterial arginine deiminase signature. Length = 409 Score = 27.9 bits (62), Expect = 0.019 Identities = 8/44 (18%), Positives = 20/44 (45%) Query: 31 WLEQHGFSSVQPQVVADGNPVGEALHDAVNAGVDVIITSGGTGI 74 ++ + SS + + + + + L + +D+I +GG I Sbjct: 298 YVLTYNPSSSKIHIKKEKARIKDVLSFYLGRKIDIIKCAGGDLI 341
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv0867c	PF03544	33	0.001	Gram-negative bacterial tonB protein
>PF03544#Gram-negative bacterial tonB protein Length = 243 Score = 33.0 bits (75), Expect = 0.001 Identities = 22/100 (22%), Positives = 28/100 (28%) Query: 221 DPAPPADLAPPAPADLAPPAPADLAPPAPADLAPPVELAVNDLPAPLGEPLPAAPAELAP 280 PA P + APADL PP P + P E P Sbjct: 45 APAQPISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPK 104 Query: 281 PADLAPASADLAPPAPADLAPPAPAELAPPAPADLAPPAA 320 P + P + P +P E PA + A Sbjct: 105 PKPVKKVEQPKRDVKPVESRPASPFENTAPARPTSSTATA 144 Score = 31.9 bits (72), Expect = 0.003 Identities = 24/129 (18%), Positives = 31/129 (24%), Gaps = 15/129 (11%) Query: 232 APADLAPPAPADLAPPAPADLAPPVELAVNDLPAPLGEPLPAAPAELAPPADLAPASADL 291 AP P + APADL PP +P P E P + P Sbjct: 40 VIELPAPAQPISVTMVAPADLEPP----------QAVQPPPEPVVEPEPEPEPIPE---- 85 Query: 292 APPAPADLAPPAPAELAPPAPADLAPPAAVNEQTAPGDQPATAPGGPVGLATDLELPEPD 351 PP A + P P P + P + +P A Sbjct: 86 -PPKEAPVVIEKPKPKPKPKPKPVKKVEQPKRDVKPVESRPASPFENTAPARPTSSTATA 144 Query: 352 PQPADAPPP 360 Sbjct: 145 ATSKPVTSV 153 Score = 31.9 bits (72), Expect = 0.004 Identities = 31/133 (23%), Positives = 44/133 (33%), Gaps = 6/133 (4%) Query: 181 DPAPPADLAPPAPADVAPPVELAVNDLPAPLGEPLPAAPADPAPPADLAPPAPADLAPPA 240 PA P + APAD+ PP AV P P+ EP P P PP AP + P Sbjct: 45 APAQPISVTMVAPADLEPP--QAVQPPPEPVVEPEPEPEPIPEPP----KEAPVVIEKPK 98 Query: 241 PADLAPPAPADLAPPVELAVNDLPAPLGEPLPAAPAELAPPADLAPASADLAPPAPADLA 300 P P P + V + + P + A++ + Sbjct: 99 PKPKPKPKPVKKVEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPR 158 Query: 301 PPAPAELAPPAPA 313 + + PA A Sbjct: 159 ALSRNQPQYPARA 171 Score = 31.9 bits (72), Expect = 0.004 Identities = 29/132 (21%), Positives = 39/132 (29%), Gaps = 2/132 (1%) Query: 147 APLAPPPADPAPPVELAANDLPAPL-GEPLPAAPADPAPPADLAPPAPADVAPPVELAVN 205 P PA P +A DL P +P P +P P + P P + AP V Sbjct: 40 VIELPAPAQPISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKE-APVVIEKPK 98 Query: 206 DLPAPLGEPLPAAPADPAPPADLAPPAPADLAPPAPADLAPPAPADLAPPVELAVNDLPA 265 P P +P+ + + APA +V P Sbjct: 99 PKPKPKPKPVKKVEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPR 158 Query: 266 PLGEPLPAAPAE 277 L P PA Sbjct: 159 ALSRNQPQYPAR 170 Score = 28.8 bits (64), Expect = 0.037 Identities = 22/130 (16%), Positives = 34/130 (26%), Gaps = 2/130 (1%) Query: 200 VELAVNDLPAPLGEP--LPAAPADPAPPADLAPPAPADLAPPAPADLAPPAPADLAPPVE 257 V +LPAP APAD PP + PP + P + P P + +E Sbjct: 36 SVHQVIELPAPAQPISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIE 95 Query: 258 LAVNDLPAPLGEPLPAAPAELAPPADLAPASADLAPPAPADLAPPAPAELAPPAPADLAP 317 + + ++ APA +A Sbjct: 96 KPKPKPKPKPKPVKKVEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVAS 155 Query: 318 PAAVNEQTAP 327 + P Sbjct: 156 GPRALSRNQP 165 Score = 28.4 bits (63), Expect = 0.042 Identities = 26/121 (21%), Positives = 32/121 (26%), Gaps = 7/121 (5%) Query: 145 EPAPLAPPPADPAPPVELAANDLPAPLGEPLPAAPADPAPPADLAPPAPADVAPPVELAV 204 PA L PP A PP E P P P P A P P Sbjct: 56 APADLEPPQAVQPPP-EPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKPVK----- 109 Query: 205 NDLPAPLGEPLPAAPADPAPPADLAPPAPADLAPPAPADLAPPAPADLAPPVELAVNDLP 264 + P + P +P + AP P A + A + P Sbjct: 110 -KVEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQPQYP 168 Query: 265 A 265 A Sbjct: 169 A 169
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv0872c	cloacin	37	1e-04	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 37.4 bits (86), Expect = 1e-04 Identities = 30/85 (35%), Positives = 40/85 (47%) Query: 427 AGGDGGAANTDSAGSSRKAFGGDGGVGGDGASALGTGGEGGIGGQGGNGGAGGLLIGNGG 486 +GGDG NT + +S GG G+G G ++ G+G GG G+G G G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 487 AGGVGGTAGAGGTGGSGGAGGAGGA 511 G GG +GG G+GG A A Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAA 86 Score = 36.6 bits (84), Expect = 3e-04 Identities = 30/88 (34%), Positives = 34/88 (38%), Gaps = 4/88 (4%) Query: 486 GAGGVGGTAGAGGTGGSGGAGGAGGAGGGGTNSGPGAAFGGNGNTGGNGGNGGAPGALGG 545 G G G GA T G+ G G GGG + G G + N GG+G G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 546 KGGSGGLIGRAGSDGGVGAGGAGGAGGA 573 G G S GG G GG A A Sbjct: 63 GNGGGN----GNSGGGSGTGGNLSAVAA 86 Score = 36.2 bits (83), Expect = 4e-04 Identities = 32/86 (37%), Positives = 37/86 (43%), Gaps = 2/86 (2%) Query: 461 GTGGEGGIGGQGGN--GGAGGLLIGNGGAGGVGGTAGAGGTGGSGGAGGAGGAGGGGTNS 518 G G G GN GG GL +G G + G G ++ GG G+G G G G N Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNG 65 Query: 519 GPGAAFGGNGNTGGNGGNGGAPGALG 544 G GG TGGN AP A G Sbjct: 66 GGNGNSGGGSGTGGNLSAVAAPVAFG 91 Score = 33.5 bits (76), Expect = 0.003 Identities = 26/67 (38%), Positives = 28/67 (41%) Query: 368 NGGSGGNGFDSFASGGTGGAGGTGGAGGRGGLLIGDGGAGGAGGVGGTGGSGAPGGGGGA 427 NGG G G AS G+G + GG G I GG G G GG G SG G GG Sbjct: 21 NGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNGNSGGGSGTGGN 80 Query: 428 GGDGGAA 434 A Sbjct: 81 LSAVAAP 87 Score = 33.1 bits (75), Expect = 0.003 Identities = 29/96 (30%), Positives = 36/96 (37%), Gaps = 9/96 (9%) Query: 484 NGGAGGVGGTAGAGGTGGSGGAGGAGGAG--------GGGTNSGPGAAFGGNGNTGGNGG 535 N GA G G TG G G + G+G GGG+ SG GG+G+ G G Sbjct: 10 NTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHW-GGGSGHGNGGGN 68 Query: 536 NGGAPGALGGKGGSGGLIGRAGSDGGVGAGGAGGAG 571 G+ G S A + GAGG Sbjct: 69 GNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 32.4 bits (73), Expect = 0.006 Identities = 29/94 (30%), Positives = 35/94 (37%), Gaps = 1/94 (1%) Query: 510 GAGGGGTNSGPGAAFGG-NGNTGGNGGNGGAPGALGGKGGSGGLIGRAGSDGGVGAGGAG 568 G G G N+G + G NG G G GGA G + G +GS G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 569 GAGGAGGTGGEGGTGGDGKTTDGNPGMGGSPGSA 602 G GG G G G G + P G P + Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALS 96 Score = 31.6 bits (71), Expect = 0.009 Identities = 25/76 (32%), Positives = 26/76 (34%) Query: 404 GGAGGAGGVGGTGGSGAPGGGGGAGGDGGAANTDSAGSSRKAFGGDGGVGGDGASALGTG 463 GG G G SG GG G GG A+ S SS G G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 464 GEGGIGGQGGNGGAGG 479 G GG G G G G Sbjct: 63 GNGGGNGNSGGGSGTG 78 Score = 31.6 bits (71), Expect = 0.011 Identities = 37/119 (31%), Positives = 47/119 (39%), Gaps = 9/119 (7%) Query: 146 GSGGVGQAGGAGGSAGLIGIGGTGGAGGAGAVGGVGGNGGWLYGNGGAGGLGGTGVAGVN 205 G G G GA ++G I GG G G GG GW N GG G+G+ Sbjct: 3 GGDGRGHNTGAHSTSGNI----NGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGG 58 Query: 206 GGMGAAGGAGGNAYLFGSGGAGGQGGMGAAGADGVNPTPTGTADAGSTGTDQTLGGNAI 264 G GG GN SGG G GG +A A V + G+ G ++ A+ Sbjct: 59 GSGHGNGGGNGN-----SGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGAL 112 Score = 30.8 bits (69), Expect = 0.019 Identities = 27/91 (29%), Positives = 36/91 (39%), Gaps = 9/91 (9%) Query: 250 AGSTGTDQTLGGNAIGGNGGPGDAGDAMTSGGAGGSGGNAVSTVNGDAVGGEGGKGGEGA 309 +G G G ++ GN G G + G + GSG ++ + G G GG Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 310 YGGAGGAGGSAASIGNAAIGGNGGAGGNAQA 340 +G GG G S GG G GGN A Sbjct: 62 HGNGGGNGNS---------GGGSGTGGNLSA 83
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv0876c	RTXTOXINA	30	0.034	Gram-negative bacterial RTX toxin determinant A family...
>RTXTOXINA#Gram-negative bacterial RTX toxin determinant A family signature. Length = 1024 Score = 29.9 bits (67), Expect = 0.034 Identities = 17/48 (35%), Positives = 25/48 (52%), Gaps = 4/48 (8%) Query: 416 TVLVTVLA-IAAAVAGSLAATAIATL---ITAGSSAIAKASLDASLQH 459 TVL +V + I+AA SL ++ L +T S I +AS A +H Sbjct: 373 TVLASVSSGISAAATTSLVGAPVSALVGAVTGIISGILEASKQAMFEH 420
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv0878c	cloacin	35	4e-04	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 35.5 bits (81), Expect = 4e-04 Identities = 29/103 (28%), Positives = 39/103 (37%), Gaps = 25/103 (24%) Query: 263 GNGNDGNTNFGSGNAGFLNIGSGNEGSGNLGFGNAGDDNTGWGNSGDTNTGGFNSGDLNT 322 G G++ + SGN N G LG G D +GW + + GG SG Sbjct: 6 GRGHNTGAHSTSGNI--------NGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGI--- 54 Query: 323 GIGSPVTQGVANSGFGNTGTGHSGFFNSGNSGSGFQNLGNGSS 365 G +G G+ G +GNSG G GN S+ Sbjct: 55 ------------HWGGGSGHGNGG--GNGNSGGGSGTGGNLSA 83 Score = 30.1 bits (67), Expect = 0.022 Identities = 27/75 (36%), Positives = 33/75 (44%), Gaps = 1/75 (1%) Query: 243 GNGNIGNANLGSGNAGFFNFGNGNDGNTNFGSGNAGFLNIGSGNEGSGNLGFGNAGDDNT 302 G G+ A+ SGN G G G + GSG + N G GSG G +G N Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNG 65 Query: 303 GW-GNSGDTNTGGFN 316 G GNSG + G N Sbjct: 66 GGNGNSGGGSGTGGN 80

Rv0973c

Rv0991c

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
Rv0973c	0	19	3.614333	Probable acetyl-/propionyl-coenzyme A
Rv0974c	2	20	3.965706	Probable acetyl-/propionyl-CoA carboxylase (beta
Rv0975c	4	19	4.729919	Probable acyl-CoA dehydrogenase FadE13
Rv0976c	5	19	5.301077	Conserved hypothetical protein
Rv0977	6	16	4.980558	PE-PGRS family protein PE_PGRS16
Rv0978c	2	13	2.246956	PE-PGRS family protein PE_PGRS17
Rv0979c	0	9	1.350047	Hypothetical protein
Rv0979A	0	9	1.261528	50S ribosomal protein L32 RpmF
Rv0980c	0	10	0.752781	PE-PGRS family protein PE_PGRS18
Rv0981	-2	12	-2.279486	Mycobacterial persistence regulator MRPA (two
Rv0982	1	24	-5.844563	Two component sensor kinase MprB
Rv0983	3	33	-8.076687	Probable serine protease PepD (serine
Rv0984	1	35	-8.957863	Possible pterin-4-alpha-carbinolamine
Rv0985c	2	35	-8.565029	Possible large-conductance ion mechanosensitive
Rv0986	3	35	-8.479391	Probable adhesion component transport
Rv0987	2	30	-6.685572	Probable adhesion component transport
Rv0988	-1	17	-2.481985	Possible conserved exported protein
Rv0989c	2	10	1.231319	Probable polyprenyl-diphosphate synthase GrcC2
Rv0990c	2	13	1.906403	Hypothetical protein
Rv0991c	2	13	1.682765	Conserved serine rich protein

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv0973c	RTXTOXIND	32	0.009	Gram-negative bacterial RTX secretion protein D signat...
>RTXTOXIND#Gram-negative bacterial RTX secretion protein D signature. Length = 478 Score = 31.7 bits (72), Expect = 0.009 Identities = 7/34 (20%), Positives = 15/34 (44%) Query: 625 TIAAPADGVLTHVSVNTGQQVEVGAILARVEAPQ 658 I + ++ + V G+ V G +L ++ A Sbjct: 98 EIKPIENSIVKEIIVKEGESVRKGDVLLKLTALG 131
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv0977	cloacin	44	2e-06	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 44.3 bits (104), Expect = 2e-06 Identities = 35/101 (34%), Positives = 43/101 (42%), Gaps = 1/101 (0%) Query: 468 AGGDGGQGDIGFDGGRGG-DGGPGGGGGAGGDGSGTFNAQANNGGDGGAGGVGGAGGTGG 526 +GGDG + G G +GGP G G GG G+ + NN GG+G GG G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 527 TGGVGADGGRGGDSGRGGDGGNAGHGGAAQFSGRGAYGGEG 567 G G +G GG SG GG+ A F G G Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG 102 Score = 43.9 bits (103), Expect = 3e-06 Identities = 37/108 (34%), Positives = 46/108 (42%), Gaps = 7/108 (6%) Query: 357 GGAGAGGDGGAGGIGGTGGNGSIGGAAGNGGNGGRGGA------GGMATAGSDGGNGGGG 410 GG G G + GA G G G G G + G G + GG + +G G G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 411 GNGGVGVGSAGGAGGTGGDGGAAGAGGAPGHGYFQQPAPQGLPIGTGG 458 GNGG G++GG GTGG+ A A A G P GL + Sbjct: 63 GNGGGN-GNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISA 109 Score = 42.0 bits (98), Expect = 9e-06 Identities = 30/82 (36%), Positives = 36/82 (43%), Gaps = 2/82 (2%) Query: 304 GIGEQGGQGGDGGA--GGAGGIGGSAGGIGGSQGAGGHGGDGGQGGAGGSGGVGGGGAGA 361 G G G G GG G+G G GS + + GG G+G G G G Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNG 65 Query: 362 GGDGGAGGIGGTGGNGSIGGAA 383 GG+G +GG GTGGN S A Sbjct: 66 GGNGNSGGGSGTGGNLSAVAAP 87 Score = 41.2 bits (96), Expect = 2e-05 Identities = 33/99 (33%), Positives = 42/99 (42%), Gaps = 1/99 (1%) Query: 158 GRGGDAGLFGHGGHGGVGGPGIAGAAGTAGLPGGNGANGGSGGIGGAGGAGGNGGLLFGN 217 GRG + G G+ G G+ G + G + N GG G+G G G Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNG 65 Query: 218 GGAGGQGGSGGLGGSGGTGGAGMAAG-PAGGTGGIGGIG 255 GG G GG G GG+ A +A G PA T G GG+ Sbjct: 66 GGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 41.2 bits (96), Expect = 2e-05 Identities = 32/79 (40%), Positives = 36/79 (45%), Gaps = 1/79 (1%) Query: 312 GGDGGAGGAGGIGGSAGGIGGSQGAGGHGGDGGQGGAGGSGGVGGGGAGAG-GDGGAGGI 370 GGDG G S GG G G GG G GGG+G+G GG G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 371 GGTGGNGSIGGAAGNGGNG 389 G GGNG+ GG +G GGN Sbjct: 63 GNGGGNGNSGGGSGTGGNL 81 Score = 40.5 bits (94), Expect = 3e-05 Identities = 30/104 (28%), Positives = 33/104 (31%) Query: 330 IGGSQGAGGHGGDGGQGGAGGSGGVGGGGAGAGGDGGAGGIGGTGGNGSIGGAAGNGGNG 389 + G G G + G G G G G G DG G G GG Sbjct: 1 MSGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGS 60 Query: 390 GRGGAGGMATAGSDGGNGGGGGNGGVGVGSAGGAGGTGGDGGAA 433 G G GG +G G GG V A T G GG A Sbjct: 61 GHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 40.1 bits (93), Expect = 4e-05 Identities = 27/80 (33%), Positives = 34/80 (42%) Query: 538 GDSGRGGDGGNAGHGGAAQFSGRGAYGGEGGSGGAGGNAGGAGTGGTAGSGGAGGFGGNG 597 G GRG + G G G G G S G+G ++ GG +GSG G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 598 ADGGNGGNGGNGGFGGINGT 617 +GG GN G G G N + Sbjct: 63 GNGGGNGNSGGGSGTGGNLS 82 Score = 39.3 bits (91), Expect = 7e-05 Identities = 36/104 (34%), Positives = 45/104 (43%), Gaps = 3/104 (2%) Query: 257 IGGAGGVGGHGSALFGHGGINGDGGTGGMGGQGGAGGNGWAAEGITVGIGEQGGQGGDGG 316 + G G G + A G ING G TG G G + G+GW++E G G G GG Sbjct: 1 MSGGDGRGHNTGAHSTSGNING-GPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGG 59 Query: 317 AGGAGGIGGSAGGIGGSQGAGGHGGDGGQGGAGGSGGVGGGGAG 360 +G GG G GG G GG+ A G + GAG Sbjct: 60 SGHGN--GGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAG 101 Score = 37.4 bits (86), Expect = 3e-04 Identities = 27/90 (30%), Positives = 35/90 (38%) Query: 558 SGRGAYGGEGGSGGAGGNAGGAGTGGTAGSGGAGGFGGNGADGGNGGNGGNGGFGGINGT 617 SG G G+ GN G TG G G + G G + + GG G+G G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 618 FGTNGAGGTGGLGTLLGGHNGNIGLNGATG 647 G G G G G+ GG+ + A G Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAAPVAFG 91 Score = 36.6 bits (84), Expect = 5e-04 Identities = 35/113 (30%), Positives = 45/113 (39%), Gaps = 14/113 (12%) Query: 492 GGGAGGDGSGTFNAQANNGGDGGAGGVGGAGGTGGTGGVGADGGRGGDSGRG-GDGGNAG 550 GG G +G + N G GVGG G + G+G + GG SG G GG +G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGG-GASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 551 HGGAAQFSGRGAYGGEGGSGGAGGNAGGAGTGGTAGSGGAGGFGGNGADGGNG 603 HG GG+G +GG +G G + A GF G G Sbjct: 62 HGNG------------GGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG 102 Score = 35.8 bits (82), Expect = 9e-04 Identities = 28/83 (33%), Positives = 34/83 (40%), Gaps = 1/83 (1%) Query: 195 NGGSGGIGGAGGAGGNGGLLFGNGGAGGQGGSG-GLGGSGGTGGAGMAAGPAGGTGGIGG 253 NGG G+G GGA G N GG GSG GG G G G GG+G G Sbjct: 21 NGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNGNSGGGSGTGGN 80 Query: 254 IGGIGGAGGVGGHGSALFGHGGI 276 + + G + G GG+ Sbjct: 81 LSAVAAPVAFGFPALSTPGAGGL 103 Score = 35.5 bits (81), Expect = 0.001 Identities = 31/80 (38%), Positives = 35/80 (43%), Gaps = 1/80 (1%) Query: 183 AGTAGLPGGNGANGGSGGI-GGAGGAGGNGGLLFGNGGAGGQGGSGGLGGSGGTGGAGMA 241 +G G GA+ SG I GG G G GG G+G + GG GSG G G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 242 AGPAGGTGGIGGIGGIGGAG 261 G GG G GG G GG Sbjct: 62 HGNGGGNGNSGGGSGTGGNL 81 Score = 35.5 bits (81), Expect = 0.001 Identities = 29/81 (35%), Positives = 33/81 (40%), Gaps = 6/81 (7%) Query: 422 GAGGTGGDGGAAGAGGAPGHGYFQQPAPQGLPIGTGGTGGEGGAGGAGGDGGQGDIGFDG 481 G G G + GA G G P GL +G G + G G + GG G Sbjct: 3 GGDGRGHNTGAHSTSGNINGG------PTGLGVGGGASDGSGWSSENNPWGGGSGSGIHW 56 Query: 482 GRGGDGGPGGGGGAGGDGSGT 502 G G G GGG G G GSGT Sbjct: 57 GGGSGHGNGGGNGNSGGGSGT 77 Score = 33.5 bits (76), Expect = 0.004 Identities = 23/77 (29%), Positives = 27/77 (35%) Query: 455 GTGGTGGEGGAGGAGGDGGQGDIGFDGGRGGDGGPGGGGGAGGDGSGTFNAQANNGGDGG 514 G G G GA G+ G G G G G G G G+ + GG G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 515 AGGVGGAGGTGGTGGVG 531 G G GG+G G Sbjct: 63 GNGGGNGNSGGGSGTGG 79 Score = 32.4 bits (73), Expect = 0.009 Identities = 26/81 (32%), Positives = 30/81 (37%), Gaps = 1/81 (1%) Query: 201 IGGAGGAGGNGGLLFGNGGA-GGQGGSGGLGGSGGTGGAGMAAGPAGGTGGIGGIGGIGG 259 + G G G N G +G GG G G GG+ G P GG G G G G Sbjct: 1 MSGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGS 60 Query: 260 AGGVGGHGSALFGHGGINGDG 280 G GG G G G+ Sbjct: 61 GHGNGGGNGNSGGGSGTGGNL 81 Score = 30.8 bits (69), Expect = 0.025 Identities = 27/74 (36%), Positives = 32/74 (43%), Gaps = 11/74 (14%) Query: 134 NGGAGGILWGNGGNGGSGAPGQPGGRGGDAGLFGHGGHGGVGGPGIAGAAGTAGLPGGNG 193 NGG G+ G G + GSG + GG +G H G G G G GGNG Sbjct: 21 NGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNG-----------GGNG 69 Query: 194 ANGGSGGIGGAGGA 207 +GG G GG A Sbjct: 70 NSGGGSGTGGNLSA 83 Score = 30.5 bits (68), Expect = 0.037 Identities = 24/71 (33%), Positives = 28/71 (39%), Gaps = 7/71 (9%) Query: 379 IGGAAGNGGNGGRGGAGGMATAGSDGGNGGGGGNGGVGVGSA----GGAGGTG---GDGG 431 + G G G N G G G G GGG + G G S GG G+G G G Sbjct: 1 MSGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGS 60 Query: 432 AAGAGGAPGHG 442 G GG G+ Sbjct: 61 GHGNGGGNGNS 71
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv0978c	cloacin	37	8e-05	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 37.0 bits (85), Expect = 8e-05 Identities = 28/90 (31%), Positives = 35/90 (38%), Gaps = 5/90 (5%) Query: 122 NGIDGTGQAGGNGGWLWGNGGNGGSGAPGQAGGAGGAAGLIGNGGAGGTGGAVSLARAGT 181 +G DG G G + +G P G GGA+ G G S + Sbjct: 2 SGGDGRGHNTGAH-----STSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHW 56 Query: 182 AGGAGRGPVGGIGGAGGVGGAGGAAGAVTT 211 GG+G G GG G +GG G GG AV Sbjct: 57 GGGSGHGNGGGNGNSGGGSGTGGNLSAVAA 86 Score = 32.0 bits (72), Expect = 0.003 Identities = 24/78 (30%), Positives = 29/78 (37%), Gaps = 6/78 (7%) Query: 117 IGDGANGIDGTGQA------GGNGGWLWGNGGNGGSGAPGQAGGAGGAAGLIGNGGAGGT 170 +G G DG+G + GG G GG G G G G +GG +G GN A Sbjct: 27 LGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNGNSGGGSGTGGNLSAVAA 86 Query: 171 GGAVSLARAGTAGGAGRG 188 A T G G Sbjct: 87 PVAFGFPALSTPGAGGLA 104 Score = 31.6 bits (71), Expect = 0.005 Identities = 32/103 (31%), Positives = 36/103 (34%), Gaps = 4/103 (3%) Query: 117 IGDGANGIDGTGQAGGNGGWLWGNGGNGGSGAPGQAGGAGGAAGLIGNGGAGG----TGG 172 I G G+ G A GW N GG G G G G G G G TGG Sbjct: 20 INGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNGNSGGGSGTGG 79 Query: 173 AVSLARAGTAGGAGRGPVGGIGGAGGVGGAGGAAGAVTTITHA 215 +S A A G G GG AG + A+ I A Sbjct: 80 NLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAAIADIMAA 122
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv0980c	cloacin	42	4e-06	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 42.0 bits (98), Expect = 4e-06 Identities = 40/121 (33%), Positives = 49/121 (40%), Gaps = 1/121 (0%) Query: 170 AGGQGLPFEAGANGGAGGAGGWLFGNGGAGGVGGAGGAGTTFGVAGGDGGTGGVGGHGGL 229 +GG G GA+ +G G G G GG G + GG G+G G G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 230 IGVGGHGGDGGTG-GTGGAVSLARAGTAGGAGGGPAGGIGGAGGVGGAGGAAGAVTTITH 288 G GG G+ G G GTGG +S A A G G GG AG + A+ I Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAAIADIMA 121 Query: 289 A 289 A Sbjct: 122 A 122 Score = 39.3 bits (91), Expect = 3e-05 Identities = 26/78 (33%), Positives = 33/78 (42%), Gaps = 1/78 (1%) Query: 139 GNGGNGGSGAPGQAGGAGGAAGLIGNGGAGGAGGQGLPFEAGANGGAGGAGGWLFGNGGA 198 G+G +GA +G G +G GG G + G G E GG G+G G G Sbjct: 4 GDGRGHNTGAHSTSGNINGGPTGLGVGG-GASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 199 GGVGGAGGAGTTFGVAGG 216 G GG G +G G G Sbjct: 63 GNGGGNGNSGGGSGTGGN 80 Score = 33.9 bits (77), Expect = 0.001 Identities = 30/117 (25%), Positives = 35/117 (29%), Gaps = 11/117 (9%) Query: 118 GDGANGIDGTGQAGGNGGWLWGNGGNGGSGAPGQAGGAGGAAGLIGNGGAGGAGGQGLPF 177 GDG G GN NGG G GGA +G G G Sbjct: 4 GDGRGHNTGAHSTSGNI--------NGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIH 55 Query: 178 EAGANGGAGGAGGWLFGNGGAGGVGGAGGAGTTFGVAGGDGGTGGVGGHGGLIGVGG 234 G +G G G GN G G G + VA G G G + + Sbjct: 56 WGGGSGHGNGGGN---GNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISA 109 Score = 33.1 bits (75), Expect = 0.003 Identities = 29/86 (33%), Positives = 35/86 (40%), Gaps = 13/86 (15%) Query: 213 VAGGDGGTGGVGGH-------GGLIGVGGHGGDGGTGGTG------GAVSLARAGTAGGA 259 ++GGDG G H GG G+G GG G G S + GG+ Sbjct: 1 MSGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGS 60 Query: 260 GGGPAGGIGGAGGVGGAGGAAGAVTT 285 G G GG G +GG G GG AV Sbjct: 61 GHGNGGGNGNSGGGSGTGGNLSAVAA 86 Score = 29.3 bits (65), Expect = 0.033 Identities = 26/78 (33%), Positives = 33/78 (42%), Gaps = 5/78 (6%) Query: 117 IGDGANGIDGTGQAGGNGGWLWGNGGN---GGSGAPGQAGGAGGAAGLIGNGGAGGAGGQ 173 +G G DG+G + N W G+G GG G GG G + G G GG A Sbjct: 27 LGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNGNSGGGSGTGGNLSAVAA 86 Query: 174 GLPF--EAGANGGAGGAG 189 + F A + GAGG Sbjct: 87 PVAFGFPALSTPGAGGLA 104
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv0981	HTHFIS	103	9e-28	FIS bacterial regulatory protein HTH signature.
>HTHFIS#FIS bacterial regulatory protein HTH signature. Length = 484 Score = 103 bits (258), Expect = 9e-28 Identities = 45/135 (33%), Positives = 71/135 (52%), Gaps = 1/135 (0%) Query: 2 RILVVDDDRAVRESLRRSLSFNGYSVELAHDGVEALDMIASDRPDALVLDVMMPRLDGLE 61 ILV DDD A+R L ++LS GY V + + IA+ D +V DV+MP + + Sbjct: 5 TILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAFD 64 Query: 62 VCRQLRGTGDDLPILVLTARDSVSERVAGLDAGADDYLPKPFALEELLARM-RALLRRTK 120 + +++ DLP+LV++A+++ + + GA DYLPKPF L EL+ + RAL + Sbjct: 65 LLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAEPKR 124 Query: 121 PEDAAESMAMRFSDL 135 E + L Sbjct: 125 RPSKLEDDSQDGMPL 139
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv0983	V8PROTEASE	57	4e-11	V8 serine protease family signature.
>V8PROTEASE#V8 serine protease family signature. Length = 336 Score = 57.3 bits (138), Expect = 4e-11 Identities = 36/182 (19%), Positives = 72/182 (39%), Gaps = 27/182 (14%) Query: 165 PSVVMLETDLGRQSEEGSGIILSAEGLILTNNHVIAAAAKPPLG---SPPPKTTVTFSDG 221 V ++ + + SG+++ + +LTN HV+ A P P + +G Sbjct: 88 APVTYIQVEAPTGTFIASGVVV-GKDTLLTNKHVVDATHGDPHALKAFPSAINQDNYPNG 146 Query: 222 RTAPFTVVGADPTSDIAVVRV----QGVS---GLTPISLGSSSDLRVGQPVLAIGSPLGL 274 + D+A+V+ Q + P ++ ++++ +V Q + G P Sbjct: 147 GFTAEQITKYSGEGDLAIVKFSPNEQNKHIGEVVKPATMSNNAETQVNQNITVTGYPGDK 206 Query: 275 EGTVTTGIVSALNRPVSTTGEAGNQNTVLD--AIQTDAAINPGNSGGALVNMNAQLVGVN 332 PV+T E+ + T L A+Q D + GNSG + N +++G++ Sbjct: 207 --------------PVATMWESKGKITYLKGEAMQYDLSTTGGNSGSPVFNEKNEVIGIH 252 Query: 333 SA 334 Sbjct: 253 WG 254
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv0985c	MECHCHANNEL	162	1e-54	Bacterial mechano-sensitive ion channel signature.
>MECHCHANNEL#Bacterial mechano-sensitive ion channel signature. Length = 136 Score = 162 bits (412), Expect = 1e-54 Identities = 47/134 (35%), Positives = 66/134 (49%), Gaps = 6/134 (4%) Query: 1 MLKGFKEFLARGNIVDLAVAVVIGTAFTALVTKFTDSIITPLINR----IGVNAQSDVGI 56 ++K F+EF RGN+VDLAV V+IG AF +V+ II P + I + Sbjct: 3 IIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPPLGLLIGGIDFKQFAVTLR 62 Query: 57 LRIGIGGGQTIDLNVLLSAAINFFLIAFAVYFLVVLPYNTLRKKGE-VEQPGDT-QVVLL 114 G + V + +F ++AFA++ + L RKK E P T + VLL Sbjct: 63 DAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKLNRKKEEPAAAPAPTKEEVLL 122 Query: 115 TEIRDLLAQTNGDS 128 TEIRDLL + N S Sbjct: 123 TEIRDLLKEQNNRS 136

Rv1034c

Rv1067c

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
Rv1034c	2	14	1.557172	Probable transposase (fragment)
Rv1035c	3	14	1.821571	Probable transposase (fragment)
Rv1036c	3	16	0.637562	Probable IS1560 transposase (fragment)
Rv1037c	2	16	0.847283	Putative ESAT-6 like protein EsxI (ESAT-6 like
Rv1038c	0	14	0.530340	ESAT-6 like protein EsxJ (ESAT-6 like protein
Rv1039c	0	17	0.505617	PPE family protein PPE15
Rv1040c	0	21	-0.914415	PE family protein PE8
Rv1041c	1	22	-1.817680	Probable is like-2 transposase
Rv1042c	2	18	-1.331050	Probable is like-2 transposase
Rv1043c	2	21	-1.868367	Conserved hypothetical protein
Rv1044	2	20	-1.440308	Conserved hypothetical protein
Rv1045	1	17	-0.947886	Hypothetical protein
Rv1046c	1	16	-0.350926	Hypothetical protein
Rv1047	1	16	-0.567101	Probable transposase
Rv1048c	0	19	-1.466406	Hypothetical protein
Rv1049	1	19	-0.492613	Probable transcriptional repressor protein
Rv1050	2	20	-1.354307	Probable oxidoreductase
Rv1051c	3	18	-2.377993	Conserved hypothetical protein
Rv1052	5	16	-1.646409	Hypothetical protein
Rv1053c	3	12	-1.692731	Hypothetical protein
Rv1054	1	11	-1.232833	Probable integrase (fragment)
Rv1055	0	11	-1.191262	Possible integrase (fragment)
Rv1056	0	10	-1.269929	*Conserved protein
Rv1057	0	10	-0.744881	Conserved hypothetical protein
Rv1058	0	10	-0.435297	Probable medium chain fatty-acid-CoA ligase
Rv1059	2	11	0.706550	Conserved protein
Rv1060	0	11	1.238346	Unknown protein
Rv1061	0	11	2.011165	Conserved protein
Rv1062	4	18	6.681685	Conserved hypothetical protein
Rv1063c	8	23	7.968431	Conserved hypothetical protein
Rv1064c	6	20	6.378571	Possible lipoprotein LpqV
Rv1065	5	18	5.670204	Conserved hypothetical protein
Rv1066	5	19	5.139772	Conserved hypothetical protein
Rv1067c	5	17	4.150237	PE-PGRS family protein PE_PGRS19

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv1043c	V8PROTEASE	47	3e-08	V8 serine protease family signature.
>V8PROTEASE#V8 serine protease family signature. Length = 336 Score = 47.3 bits (112), Expect = 3e-08 Identities = 39/164 (23%), Positives = 65/164 (39%), Gaps = 29/164 (17%) Query: 145 GTGLVVDHNHVITNKHVVTGLAGTSAGLSVYPSSNHAEA---------ELVNFSGTAHPH 195 +G+VV + ++TNKHVV G L +PS+ + + ++ +SG Sbjct: 104 ASGVVVGKDTLLTNKHVVDATHGDPHALKAFPSAINQDNYPNGGFTAEQITKYSGEG--- 160 Query: 196 PTLDVAVIKFEMPE-----GKYIPRLGGMAFRDPDWADEVYVFGYPRVPMTAEMAITVQR 250 D+A++KF E G+ + + + V GYP A M + Sbjct: 161 ---DLAIVKFSPNEQNKHIGEVVKPATMSNNAETQVNQNITVTGYPGDKPVATMW--ESK 215 Query: 251 GEVVNPAATTIPGRQKIFLYSAIARPGNSGGPIVAQDGRVIGLV 294 G++ T + G Y GNSG P+ + VIG+ Sbjct: 216 GKI-----TYLKGE--AMQYDLSTTGGNSGSPVFNEKNEVIGIH 252
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv1050	DHBDHDRGNASE	84	4e-21	2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase signature. Length = 261 Score = 83.9 bits (207), Expect = 4e-21 Identities = 53/187 (28%), Positives = 84/187 (44%), Gaps = 1/187 (0%) Query: 9 QVVLITGASSGIGEATAKAFAREGAVVALAARREGALRRVAREIEAAGGRAMVAPLDVSS 68 ++ ITGA+ GIGEA A+ A +GA +A L +V ++A A P DV Sbjct: 9 KIAFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHAEAFPADVRD 68 Query: 69 SESVRAMVADVVGEFGRIDVVFNNAGVSLVGPVDAETFLDDTREMLEIDYLGTVRVVREV 128 S ++ + A + E G ID++ N AGV G + ++ ++ G R V Sbjct: 69 SAAIDEITARIEREMGPIDILVNVAGVLRPGLIH-SLSDEEWEATFSVNSTGVFNASRSV 127 Query: 129 LPIMKQQRSGRIMNMSSVVGRKAFARFAGYSSAMHAIAGFSDALRQELRGSGIAVSVIHP 188 M +RSG I+ + S A Y+S+ A F+ L EL I +++ P Sbjct: 128 SKYMMDRRSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLELAEYNIRCNIVSP 187 Query: 189 ALTQTPL 195 T+T + Sbjct: 188 GSTETDM 194
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv1067c	cloacin	37	2e-04	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 37.4 bits (86), Expect = 2e-04 Identities = 36/102 (35%), Positives = 45/102 (44%), Gaps = 1/102 (0%) Query: 303 GAGGRGGDGGSAGWLSG-NGGDAGNGGGGGTAGGAGNGGQFGGDGGTGGTGGTAGAGGNG 361 G GRG + G+ NGG G G GGG + G+G + GG G+G G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 362 GRGAVLFGHGGNAGHGGAGGNGAAAGAGGEHVVATAGKGGTG 403 G G GG +G GG AA A G ++T G GG Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 37.4 bits (86), Expect = 2e-04 Identities = 33/82 (40%), Positives = 41/82 (50%), Gaps = 4/82 (4%) Query: 512 TGNAGNGGNGG--SAARLFGGGGAGGAGGTGSTAGSGGSGGTNPPTGLQAAGGNGGSGHA 569 +G G G N G S + GG G G G++ GSG S NP G +G + G G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 570 GGHGGNGGGAGLLGGGGTGGNG 591 G+GG G +G GG GTGGN Sbjct: 62 HGNGGGNGNSG--GGSGTGGNL 81 Score = 37.0 bits (85), Expect = 3e-04 Identities = 30/89 (33%), Positives = 37/89 (41%), Gaps = 1/89 (1%) Query: 475 GAGGSGGSAGAGGAGGKGGDTPNGLAINPGIG-GNGGDTGNAGNGGNGGSAARLFGGGGA 533 G G G + GA G P GL + G G+G + N GG GS GG G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 534 GGAGGTGSTAGSGGSGGTNPPTGLQAAGG 562 G GG G++ G G+GG A G Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFG 91 Score = 36.6 bits (84), Expect = 3e-04 Identities = 37/101 (36%), Positives = 44/101 (43%), Gaps = 8/101 (7%) Query: 119 GADGTAANPNGGAGGLLYGNGGNGFSQTTAGLTGGTGGSAGLIGNGGNGGAGGAGANGGA 178 GA T+ N NGG GL G G + S ++ GGS I GG G G G NG + Sbjct: 12 GAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNGNS 71 Query: 179 GGNGGWLYGSGGNGGAGGAGPAGAIGAPGVAGGAGGAGGTA 219 GG GSG G A G P ++ GAGG A Sbjct: 72 GG------GSGTGGNLSAVAAPVAFGFPALS--TPGAGGLA 104 Score = 35.1 bits (80), Expect = 0.001 Identities = 34/100 (34%), Positives = 40/100 (40%), Gaps = 3/100 (3%) Query: 299 GGAGGAGGRGGDGGSAGWLSGNGGDAGNGGGGGTAGGAGNGGQFGGDGGTGGTGGTAGAG 358 GG G G GG +GW S N GGG G+ G G G GG G +GG +G G Sbjct: 22 GGPTGLGVGGGASDGSGWSSENNP---WGGGSGSGIHWGGGSGHGNGGGNGNSGGGSGTG 78 Query: 359 GNGGRGAVLFGHGGNAGHGGAGGNGAAAGAGGEHVVATAG 398 GN A G A G A + + G A A Sbjct: 79 GNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAAIAD 118 Score = 34.3 bits (78), Expect = 0.002 Identities = 34/117 (29%), Positives = 39/117 (33%), Gaps = 8/117 (6%) Query: 344 GDGGTGGTGGTAGAGGNGGRGAVLFGHGGNAGHGGAGGNGAAAGAGGEHVVATAGKGGTG 403 G G G G GN +GG G G GG +G E+ G G Sbjct: 3 GGDGRGHNTGAHSTSGNI--------NGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGI 54 Query: 404 GVGGDGGGGGAGGGGGLLYGNGGAGGAGNSGGDGGTGLNAALGGNGGGGGVGGNAGA 460 GG G G GG G G+G G G A GG V +AGA Sbjct: 55 HWGGGSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGA 111 Score = 34.3 bits (78), Expect = 0.002 Identities = 31/77 (40%), Positives = 36/77 (46%) Query: 529 GGGGAGGAGGTGSTAGSGGSGGTNPPTGLQAAGGNGGSGHAGGHGGNGGGAGLLGGGGTG 588 GG G G G ST+G+ G T G A+ G+G S GG G GGG Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 589 GNGGGGGQGGLGAAAGG 605 GNGGG G G G+ GG Sbjct: 63 GNGGGNGNSGGGSGTGG 79 Score = 33.9 bits (77), Expect = 0.002 Identities = 37/111 (33%), Positives = 45/111 (40%), Gaps = 11/111 (9%) Query: 374 AGHGGAGGNGAAAGAGGEHVVATAGKGGTGGVGGDGGGGGA-----GGGGGLLYGNGGAG 428 +G G G N A G ++ G GG DG G + GGG G GG Sbjct: 2 SGGDGRGHNTGAHSTSG-NINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGS 60 Query: 429 GAGNSGGDGGTGLNAALGGNGGGGGVGGNAGAGGTGGSAGWLSGNGGAGGS 479 G GN GG+G +G GG+G GG + A G A G GG S Sbjct: 61 GHGNGGGNGNSG-----GGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVS 106 Score = 33.1 bits (75), Expect = 0.003 Identities = 26/91 (28%), Positives = 36/91 (39%) Query: 555 TGLQAAGGNGGSGHAGGHGGNGGGAGLLGGGGTGGNGGGGGQGGLGAAAGGVDGNGGNGG 614 +G G N G+ G+ G +GGG + G+G G +G GG G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 615 NGGKGGDAQLVGDGGNGGNGGKGGAGLIAGL 645 +G GG+ G G GGN A + G Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAAPVAFGF 92 Score = 31.6 bits (71), Expect = 0.010 Identities = 26/74 (35%), Positives = 30/74 (40%), Gaps = 1/74 (1%) Query: 153 GTGGSAGLIGNGGNGGAGGAGANGGAGGNGGWLYGSGGNGGAGGAGPA-GAIGAPGVAGG 211 G A NGG G G GGA GW + GG G+G G G GG Sbjct: 8 GHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGG 67 Query: 212 AGGAGGTAGLFGNG 225 G +GG +G GN Sbjct: 68 NGNSGGGSGTGGNL 81 Score = 31.6 bits (71), Expect = 0.012 Identities = 35/120 (29%), Positives = 42/120 (35%), Gaps = 16/120 (13%) Query: 426 GAGGAGNSGGDGGTGLNAALGGNGGGGGVGGNAGAGGTGGSAGWLSGNGGAGGSGGSAGA 485 G G G++ G T N NGG G+G GA G + + GG GSG G Sbjct: 3 GGDGRGHNTGAHSTSGNI----NGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGG 58 Query: 486 GGAGGKGGDTPNGLAINPGIGGNGGDTGNAGNGGNGGSAARLFGGGGAGGAGGTGSTAGS 545 G G GG G G+ G AA + G A G G A S Sbjct: 59 GSGHGNGG------------GNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVS 106 Score = 31.2 bits (70), Expect = 0.014 Identities = 27/78 (34%), Positives = 32/78 (41%), Gaps = 1/78 (1%) Query: 576 GGGAGLLGGGGTGGNGGGGGQGGLGAAAGGVDGNG-GNGGNGGKGGDAQLVGDGGNGGNG 634 G G G G + GG GLG G DG+G + N GG + GG G+G Sbjct: 4 GDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHG 63 Query: 635 GKGGAGLIAGLDGAGGAG 652 GG G G G GG Sbjct: 64 NGGGNGNSGGGSGTGGNL 81 Score = 30.8 bits (69), Expect = 0.020 Identities = 32/96 (33%), Positives = 39/96 (40%), Gaps = 10/96 (10%) Query: 406 GGDGGGGGAGGGGGLLYGNGGAGGAG-NSGGDGGTGLNAALGGNGGGGGVGGNAGAGGTG 464 GGDG G G NGG G G G G+G ++ GGG G G + G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGS-- 60 Query: 465 GSAGWLSGNGGAGGSGGSAGAGGAGGKGGDTPNGLA 500 G+G GG+G S G G GG +A Sbjct: 61 -------GHGNGGGNGNSGGGSGTGGNLSAVAAPVA 89 Score = 30.8 bits (69), Expect = 0.022 Identities = 29/91 (31%), Positives = 35/91 (38%), Gaps = 3/91 (3%) Query: 168 GAGGAGANGGAGGNGGWLYG-SGGNGGAGGAGPAGAIGAPGVAGGAGGAGGTAGLFGNGG 226 G G G N GA G + G G G GGA + G GG+G G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWG-GGSGSGIHWGGGSG 61 Query: 227 VGGVGGDGGQGGNGAGAGASGTKGGDAGAGG 257 G GG G G G+G G + + A G Sbjct: 62 HGN-GGGNGNSGGGSGTGGNLSAVAAPVAFG 91 Score = 30.8 bits (69), Expect = 0.022 Identities = 31/97 (31%), Positives = 36/97 (37%), Gaps = 5/97 (5%) Query: 210 GGAGGAGGTAGLFGNGGVGGVGGDGGQGGNGAGAGAS---GTKGGDAGAGGAGGAGGWIH 266 G GA T+G G G G G G+G + + G G GG G G Sbjct: 8 GHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGG 67 Query: 267 GHGGAGGDGGAGGAGGQASPGAPGPP--SQPGGAGGA 301 GG G G A+P A G P S PG G A Sbjct: 68 NGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 30.5 bits (68), Expect = 0.024 Identities = 31/113 (27%), Positives = 41/113 (36%), Gaps = 5/113 (4%) Query: 228 GGVGGDGGQGGNGAGAGASGTKGGDAGAGGAGGAGGWIHGHGGAGGDGGAGGAGGQASPG 287 GG G G + +G G GGA GW + GG G+G G S Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 288 APGPPSQPGGAGGAGGAGGRGGDGGSAGWLSGNGGDAGNGGGGGTAGGAGNGG 340 G GG G +GG G GG+ + G A + G G + + G Sbjct: 63 GNG-----GGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAG 110 Score = 30.1 bits (67), Expect = 0.029 Identities = 23/84 (27%), Positives = 28/84 (33%) Query: 253 AGAGGAGGAGGWIHGHGGAGGDGGAGGAGGQASPGAPGPPSQPGGAGGAGGAGGRGGDGG 312 +G G G G G G G GG AS G+ GG+G GG G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 313 SAGWLSGNGGDAGNGGGGGTAGGA 336 G+G GG + A Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVA 85

Rv1082

Rv1091

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
Rv1082	3	15	5.513002	Mycothiol conjugate amidase Mca (mycothiol
Rv1083	4	13	5.884572	Conserved hypothetical protein
Rv1084	4	13	5.905886	Conserved protein
Rv1085c	7	17	5.383973	Possible hemolysin-like protein
Rv1086	8	19	6.050968	Short (C15) chain Z-isoprenyl diphosphate
Rv1087	8	19	6.216310	PE-PGRS family protein PE_PGRS21
Rv1087A	13	21	7.110871	Conserved hypothetical protein
Rv1088	11	18	5.728370	PE family protein PE9
Rv1089	7	18	5.098299	PE family protein PE10
Rv1089A	3	17	4.448019	Probable cellulase CelA2a
Rv1090	1	15	3.877557	Probable cellulase CelA2b
Rv1091	1	14	3.697104	PE-PGRS family protein PE_PGRS22

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv1087	cloacin	37	3e-04	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 36.6 bits (84), Expect = 3e-04 Identities = 31/102 (30%), Positives = 39/102 (38%), Gaps = 1/102 (0%) Query: 446 GDSGEGGFGGPGLAGGLFGNPGNGGVGGIGGDAAAGGAGGAGGNGGAGGNGGWLFGNGGA 505 GD G +G + G P GVGG D + + GG+G W G G Sbjct: 4 GDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHW-GGGSGH 62 Query: 506 GGSGGDGGAAGRGGAGNLGSAGGINAPAGNPGSGSVGIGGAG 547 G GG+G + G G G SA G P + G GG Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 36.6 bits (84), Expect = 4e-04 Identities = 30/80 (37%), Positives = 34/80 (42%), Gaps = 4/80 (5%) Query: 587 GAMGGAGGVGGNARLLGTGGAGGVGGGGGAGGDGGRGGVATPGGQGGDAGDGGAGGAGGN 646 G GA GN GG G+G GGGA G P G G +G GG+G Sbjct: 8 GHNTGAHSTSGNI----NGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHG 63 Query: 647 GGGASGAGGWLLGTGGAGGA 666 GG +G G GTGG A Sbjct: 64 NGGGNGNSGGGSGTGGNLSA 83 Score = 36.2 bits (83), Expect = 5e-04 Identities = 32/83 (38%), Positives = 36/83 (43%), Gaps = 4/83 (4%) Query: 352 LSGGDGGA--GGAGGAGGA--GGTGGWLYGGGGAAGSGGDGGTGGQGGAGGAGVFSLFGS 407 +SGGDG GA G GG G GGG + GSG GG G+G+ GS Sbjct: 1 MSGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGS 60 Query: 408 GGGPGGNGGVGGVGGVGGAGGRA 430 G G GG G G G G A Sbjct: 61 GHGNGGGNGNSGGGSGTGGNLSA 83 Score = 35.8 bits (82), Expect = 6e-04 Identities = 38/116 (32%), Positives = 44/116 (37%), Gaps = 3/116 (2%) Query: 538 SGSVGIGGAGGAGGTAGLFGDGGAGGAGGAGAAGGFGGISAATPSAGSEGAMGGAGGVGG 597 SG G G GA T+G G G G GA+ G G S P G G+ GG G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 598 NARLLGTGGAGGVGGGGGAGGDGGRGGVATPGGQGGDAGDGGAGGAGGNGGGASGA 653 + GG G GGG G GG+ G + G G A GA A Sbjct: 62 HG---NGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSA 114 Score = 35.8 bits (82), Expect = 6e-04 Identities = 35/103 (33%), Positives = 44/103 (42%), Gaps = 4/103 (3%) Query: 482 GAGGAGGNGGAGGNGGWLFGNGGAGGSGGDGGAAGRGGAGNLGSAGGINAPAGNPGSGSV 541 G G G N GA G + NGG G G GGA+ G + + G + +G G Sbjct: 3 GGDGRGHNTGAHSTSGNI--NGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGS 60 Query: 542 GIGGAGGAGGTAGLFGDGGAGGAGGAGAAGGFGGISAATPSAG 584 G G GG G + G G G G A FG + +TP AG Sbjct: 61 GHGNGGGNGNSGG--GSGTGGNLSAVAAPVAFGFPALSTPGAG 101 Score = 35.1 bits (80), Expect = 0.001 Identities = 29/88 (32%), Positives = 32/88 (36%) Query: 258 GVGGAGGVGGAGGAGGWLYGDAGAGGDGGVGGAGGTGGLGNRGGAGGAGGAGGVGGAGGA 317 G G G GA G + G G GG G N GG+G GG G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 318 AGLWGGGGAGGVGGTGGGAGLGAQSVTF 345 G G +GG GTGG A V F Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAF 90 Score = 34.7 bits (79), Expect = 0.001 Identities = 34/108 (31%), Positives = 43/108 (39%), Gaps = 5/108 (4%) Query: 616 AGGDGGRGGVATPGGQGGDAGDGGAGGAGGNGGGASGAGGWLLGTGGAGGAGGNGGNGGK 675 +GGDG G T +GG G G GG + G+G GG+G GG Sbjct: 2 SGGDGR--GHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGG 59 Query: 676 AGFSPGPTNFGLNGAGGGGGVGGNGATGPWLFGDGGPTPGSTGAGAAG 723 +G G N +GGG G GGN + G P + GAG Sbjct: 60 SGHGNGGGN---GNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 34.3 bits (78), Expect = 0.002 Identities = 38/112 (33%), Positives = 42/112 (37%), Gaps = 12/112 (10%) Query: 304 GAGGAGGVGGAGGAAGLWGGGGAGGVGGTGGGAGLGAQSVTFSSSLSGLSGGDGGAGGAG 363 G G G GA +G GG G G G G SG S + GG Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDG------------SGWSSENNPWGGGS 50 Query: 364 GAGGAGGTGGWLYGGGGAAGSGGDGGTGGQGGAGGAGVFSLFGSGGGPGGNG 415 G+G G G GGG SGG GTGG A A V F + PG G Sbjct: 51 GSGIHWGGGSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG 102 Score = 34.3 bits (78), Expect = 0.002 Identities = 29/95 (30%), Positives = 40/95 (42%), Gaps = 3/95 (3%) Query: 669 NGGNGGKAGFSPGPTNFGLNGAGGGGGVGGNGATGPWLFGDGGPTPGSTGAGAAGGHGGD 728 +GG+G T+ +NG G GVGG + G + P G +G+G G G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 729 AQLIGNGGHGGAGGTGVPNGSGGAGGLSGLLFGEP 763 GNGG G G G G + + + FG P Sbjct: 62 H---GNGGGNGNSGGGSGTGGNLSAVAAPVAFGFP 93 Score = 33.9 bits (77), Expect = 0.002 Identities = 29/83 (34%), Positives = 34/83 (40%) Query: 236 AGGAGVAGAGGFEGTIGAGGAGGVGGAGGVGGAGGAGGWLYGDAGAGGDGGVGGAGGTGG 295 +GG G G T G G G G G + G+G + GG G GG G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 296 LGNRGGAGGAGGAGGVGGAGGAA 318 GN GG G +GG G GG A Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAV 84 Score = 33.9 bits (77), Expect = 0.003 Identities = 40/112 (35%), Positives = 45/112 (40%), Gaps = 4/112 (3%) Query: 157 GGNGGAAGLIGNGGFGGGGGPGAAGGNGGAGGWLFGNGGAGGAGGLGVAPGVPGGAGGAG 216 G N GA GN G G G + G+G W N GG G G+ G G G G Sbjct: 8 GHNTGAHSTSGNINGGPTGLGVGGGASDGSG-WSSENNPWGGGSGSGIHWGGGSGHGNGG 66 Query: 217 GAGGVGGPAGLWGHGGAGGAGGAGVAGAGGFEGTIGAGGAGGVGGAGGVGGA 268 G G GG + G GG A A VA T GAGG AG + A Sbjct: 67 GNGNSGGGS---GTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAA 115 Score = 33.1 bits (75), Expect = 0.005 Identities = 38/105 (36%), Positives = 42/105 (40%), Gaps = 11/105 (10%) Query: 123 GDGANGGPGQ-----DGGPGGLLYGNGGNGGTSTTVGMAGGNGGAAGLIGNGGFGGGGGP 177 G G N G +GGP GL G GG S G + N G G+G GGG Sbjct: 6 GRGHNTGAHSTSGNINGGPTGL----GVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 178 GAAGGNGGAGGWLFGNGGAGGAGGLGVAPGVPGGAGGAGGAGGVG 222 GG G G G GG A VA G P A GAGG+ Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFP--ALSTPGAGGLA 104
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv1091	cloacin	39	8e-05	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 38.9 bits (90), Expect = 8e-05 Identities = 35/99 (35%), Positives = 43/99 (43%), Gaps = 5/99 (5%) Query: 665 GAGGNGGPGGSGGAADIGGNGGAGNGGGTDGNG---GNGGSGGGAGSGGDGGGAGGNGAW 721 G G N G + G + G G GG +DG+G N GGG+GSG GG G+G Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNG 65 Query: 722 LFGNGGAGGGGGKGGNGAGGGLGGGSFGLPGLNGSGGDG 760 G G GGG G +FG P L+ G G Sbjct: 66 --GGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG 102 Score = 37.4 bits (86), Expect = 2e-04 Identities = 29/82 (35%), Positives = 36/82 (43%), Gaps = 3/82 (3%) Query: 309 NGGSGGTGGAGGSTAGAGGNGGAGGGGGTGGLLFGNGGAGGHGAAAGNGLAAGNGVSSSG 368 +GG G G + NGG G G GG + G+G G +G+G+ G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGG---ASDGSGWSSENNPWGGGSGSGIHWGG 58 Query: 369 GGGAGGTGGAGGDGGAGGAGGN 390 G G G GG G GG G GGN Sbjct: 59 GSGHGNGGGNGNSGGGSGTGGN 80 Score = 36.6 bits (84), Expect = 4e-04 Identities = 29/76 (38%), Positives = 35/76 (46%) Query: 480 AGAGGRGGAGGSGGSGGDGGGGAAGPAGWLFGDGGAGGNGGAAAAGGAGGQAGGGGGNGG 539 GA G G +G GGGA+ +GW + GG G+ G G G GGGNG Sbjct: 11 TGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNGN 70 Query: 540 NGGNGGNGGNGGNGAT 555 +GG G GGN A Sbjct: 71 SGGGSGTGGNLSAVAA 86 Score = 36.2 bits (83), Expect = 6e-04 Identities = 38/113 (33%), Positives = 47/113 (41%), Gaps = 6/113 (5%) Query: 631 GAGGTGFISSDGGAGGDGGDGGNGGAGGTGGLLFGAGGNGGPGGSGGAADIGGNGGAGNG 690 G G S G G G G G + G + + N GGSG GG G GNG Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNG 65 Query: 691 GGTDGNGGNGGSGGGAGSGGDGGGAGGNGAWLFGNGGAGGGGGKGGNGAGGGL 743 GGNG SGGG+G+GG+ A+ F G GG + + G L Sbjct: 66 ------GGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGAL 112 Score = 36.2 bits (83), Expect = 6e-04 Identities = 28/85 (32%), Positives = 30/85 (35%) Query: 708 SGGDGGGAGGNGAWLFGNGGAGGGGGKGGNGAGGGLGGGSFGLPGLNGSGGDGGDGGNGA 767 SGGDG G GN G G G GA G G S P GSG GG Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 768 PGGVLYGNGGAGGQGSSGGIGGPGA 792 G GG G+ G + A Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAA 86 Score = 35.8 bits (82), Expect = 8e-04 Identities = 37/102 (36%), Positives = 39/102 (38%), Gaps = 1/102 (0%) Query: 302 GAGGAGGNGGSGGTGG-AGGSTAGAGGNGGAGGGGGTGGLLFGNGGAGGHGAAAGNGLAA 360 G G G N G+ T G G G G GGA G G GG G G G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 361 GNGVSSSGGGGAGGTGGAGGDGGAGGAGGNARLWGVGGAGGA 402 GNG + GG GTGG A A G L G G A Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 35.5 bits (81), Expect = 0.001 Identities = 39/130 (30%), Positives = 45/130 (34%), Gaps = 9/130 (6%) Query: 353 AAGNGLAAGNGVSSSGGGGAGGTGGAGGDGGAGGAGGNARLWGVGGAGGAGGDGGAGGAG 412 + G+G G S+ G GG G G GGA G W GG G G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSG----WSSENNPWGGGSGSGIHWG 57 Query: 413 GKGGSGLSGNANGGAGGDSGRGGTGGAGGEGGAAGLLVGTGGHGGDGGAGGAAVKGGDGG 472 G G G NGG G+SG G G AA + G G G A Sbjct: 58 GGSGHG-----NGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGAL 112 Query: 473 AAAGTGIAGA 482 +AA I A Sbjct: 113 SAAIADIMAA 122 Score = 34.7 bits (79), Expect = 0.002 Identities = 32/94 (34%), Positives = 41/94 (43%), Gaps = 10/94 (10%) Query: 533 GGGGNGGNGGNGGNGGNGGNGATGGWLYGNGGAGGQGATAGAGGAGANGVSSTNGGGTGG 592 GG G G N G GN G TG G GG + G+G + ++ GGG+G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGL-----GVGGG-----ASDGSGWSSENNPWGGGSGS 52 Query: 593 NGGIGGTGGSGGAGGNAGLLGVGGAGGHGASGGA 626 GG G G GGN G G GG+ ++ A Sbjct: 53 GIHWGGGSGHGNGGGNGNSGGGSGTGGNLSAVAA 86 Score = 34.3 bits (78), Expect = 0.002 Identities = 33/114 (28%), Positives = 45/114 (39%), Gaps = 11/114 (9%) Query: 479 IAGAGGRGGAGGSGGSGGDGGGGAAGPAGWLFGDGGAGGNGGAAAAGGAGGQAG-GGGGN 537 ++G GRG G+ + G+ GG G G GGA+ G + GGG+ Sbjct: 1 MSGGDGRGHNTGAHSTSGNINGGP----------TGLGVGGGASDGSGWSSENNPWGGGS 50 Query: 538 GGNGGNGGNGGNGGNGATGGWLYGNGGAGGQGATAGAGGAGANGVSSTNGGGTG 591 G GG G+G G G G+G G A A G +S+ GG Sbjct: 51 GSGIHWGGGSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 33.9 bits (77), Expect = 0.003 Identities = 39/118 (33%), Positives = 45/118 (38%), Gaps = 18/118 (15%) Query: 511 GDGGAGGNGGAAAAGG--AGGQAGGGGGNGGNGGNGGNGGNGGNGATGGWLYGNGGAGGQ 568 G G G N GA + G GG G G G G + G+G + N G G GG G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 569 GATAGAGGAGANGVSSTNGGGTGGNGGIGGTGGSGGAGGNAGLLGVGGAGGHGASGGA 626 G NGGG G +GG GTGG+ A G GA G A Sbjct: 63 G----------------NGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 33.9 bits (77), Expect = 0.003 Identities = 23/78 (29%), Positives = 33/78 (42%) Query: 343 GNGGAGGHGAAAGNGLAAGNGVSSSGGGGAGGTGGAGGDGGAGGAGGNARLWGVGGAGGA 402 G G G + +GN G+ GG G + + GG+G G G G Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNG 65 Query: 403 GGDGGAGGAGGKGGSGLS 420 GG+G +GG G GG+ + Sbjct: 66 GGNGNSGGGSGTGGNLSA 83 Score = 33.5 bits (76), Expect = 0.003 Identities = 25/80 (31%), Positives = 33/80 (41%) Query: 246 GGNGGAGGAAGLFGDAGAGGNGGKGGAGGAAFSINFTAGDGGAGGAGGSGGHALLWGAGG 305 G N GA +G G G G + G+ +S GG+G GG + GG Sbjct: 8 GHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGG 67 Query: 306 AGGNGGSGGTGGAGGSTAGA 325 G +GG GTGG + A Sbjct: 68 NGNSGGGSGTGGNLSAVAAP 87 Score = 33.1 bits (75), Expect = 0.005 Identities = 38/135 (28%), Positives = 49/135 (36%), Gaps = 11/135 (8%) Query: 261 AGAGGNGGKGGAGGAAFSINFTAGDGGAGGAGGSGGHALLWGAGGAGGNGGSGGTGGAGG 320 +G G G GA + +IN G GG G W + GGSG GG Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSG---WSSENNPWGGGSGSGIHWGG 58 Query: 321 STAGAGGNGGAGGGGGTGGLLFGNGGAGGHGAAAGNGLAAGNGVSSSGGGGAGGTGGAGG 380 + G G GGG+G GG+ +A +A G S+ G G + G Sbjct: 59 GSGHGNGGGNGNSGGGSGT--------GGNLSAVAAPVAFGFPALSTPGAGGLAVSISAG 110 Query: 381 DGGAGGAGGNARLWG 395 A A A L G Sbjct: 111 ALSAAIADIMAALKG 125 Score = 33.1 bits (75), Expect = 0.005 Identities = 35/112 (31%), Positives = 41/112 (36%), Gaps = 11/112 (9%) Query: 725 NGGAGGGGGKGGNGAGGGLGGGSFGLPGLNGSGGDGGDGGNGAPGGVLYGNGGAGGQGSS 784 +GG G G G + G + GG +G G G + G N GG GS Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGP--------TGLGVGGGASDGSGWSSENNPWGGGSGSG 53 Query: 785 GGIGGPGATGGAGGKGGDGGDAQLIGDGGNGGNGGAGGTGGTPGPGGPGGSG 836 GG G GG G GG + G GGN A G P PG G Sbjct: 54 IHWGGGSGHGNGGGNGNSGGGS---GTGGNLSAVAAPVAFGFPALSTPGAGG 102 Score = 33.1 bits (75), Expect = 0.005 Identities = 25/62 (40%), Positives = 30/62 (48%), Gaps = 3/62 (4%) Query: 127 NGGPGQDGGPGGLLYGNGGNGGTSTTAGVAGGNGGAAGLIGNGGAGGGGGAGAAGGNGGA 186 NGGP G GG + G+G +S GG+G G G G GGG G +GG G Sbjct: 21 NGGPTGLGVGGG---ASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNGNSGGGSGT 77 Query: 187 GG 188 GG Sbjct: 78 GG 79 Score = 31.2 bits (70), Expect = 0.020 Identities = 30/87 (34%), Positives = 37/87 (42%), Gaps = 6/87 (6%) Query: 601 GSGGAGGNAGLLGVGGAGGHGASGGAGDRGGAGGTGFISSDGGAGGDGGDGGNGGAGGTG 660 G G G N G G G +G G + G+G+ S + GG G G + G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGS-- 60 Query: 661 GLLFGAGGNGGPGGSGGAADIGGNGGA 687 G G GG G SGG + GGN A Sbjct: 61 ----GHGNGGGNGNSGGGSGTGGNLSA 83 Score = 30.8 bits (69), Expect = 0.024 Identities = 37/121 (30%), Positives = 43/121 (35%), Gaps = 13/121 (10%) Query: 564 GAGGQGATAGAGGAGANGVSSTNGGGTGGNGGIGGTGGSGGAGGNAGLLGVGGAGGHGAS 623 G G+G GA N NGG TG G G + GSG + N G G+G H Sbjct: 3 GGDGRGHNTGAHSTSGN----INGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIH--W 56 Query: 624 GGAGDRGGAGGTGFISSDGGAGGDGGDGGNGGAGGTGGLLFGAGGNGGPGGSGGAADIGG 683 GG G GG G G G G + + FG PG G A I Sbjct: 57 GGGSGHGNGGG-------NGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISA 109 Query: 684 N 684 Sbjct: 110 G 110 Score = 30.5 bits (68), Expect = 0.031 Identities = 34/89 (38%), Positives = 37/89 (41%), Gaps = 6/89 (6%) Query: 429 GDSGRGGTGGAGG-----EGGAAGLLVGTGGHGGDG-GAGGAAVKGGDGGAAAGTGIAGA 482 G GRG GA GG GL VG G G G + GG G G +G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 483 GGRGGAGGSGGSGGDGGGGAAGPAGWLFG 511 G GG G SGG G GG +A A FG Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFG 91

Rv1234

Rv1242

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
Rv1234	-3	13	-3.219081	Probable transmembrane protein
Rv1235	-1	12	-2.528035	Probable sugar-binding lipoprotein LpqY
Rv1236	0	12	-3.167634	Probable sugar-transport integral membrane
Rv1237	1	11	-3.050139	Probable sugar-transport integral membrane
Rv1238	0	12	0.804890	Probable sugar-transport ATP-binding protein ABC
Rv1239c	0	13	2.794270	Possible magnesium and cobalt transport
Rv1240	2	14	3.613125	Probable malate dehydrogenase Mdh
Rv1241	3	14	3.608351	Possible antitoxin VapB33
Rv1242	3	14	3.488280	Possible toxin VapC33. Contains PIN domain.

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv1235	MALTOSEBP	49	2e-08	Maltose binding protein signature.
>MALTOSEBP#Maltose binding protein signature. Length = 396 Score = 49.3 bits (117), Expect = 2e-08 Identities = 43/162 (26%), Positives = 70/162 (43%), Gaps = 16/162 (9%) Query: 139 WNHKLYAAPVTTNTQLLWYRPDLVNSPPTDWNAMIAEAARLHAAGEPSWIAVQANQGEGL 198 +N KL A P+ L Y DL+ +PP W + A L A G+ A+ N E Sbjct: 125 YNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKS---ALMFNLQEPY 181 Query: 199 VVWFNTLLVSAGGSVLS-EDGRHVTL---TDTPAHRAATVSALQILKSVATTPGADPSIT 254 W L+ + GG E+G++ D +A + ++K+ D SI Sbjct: 182 FTW--PLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSI- 238 Query: 255 RTEEGSARLAFEQGKAALEVNWPFVFASMLENAVKGGVPFLP 296 A AF +G+ A+ +N P+ ++++ + V GV LP Sbjct: 239 ------AEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLP 274
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv1238	PF05272	35	4e-04	Virulence-associated E family protein
>PF05272#Virulence-associated E family protein Length = 892 Score = 35.4 bits (81), Expect = 4e-04 Identities = 24/122 (19%), Positives = 39/122 (31%), Gaps = 35/122 (28%) Query: 33 LILVGPSGCGKTTTLNMIAGLEDISSGELRIAGERVNEKAPKDRDIAMVFQSYALYPHMT 92 ++L G G GK+T +N + GL+ S I +D Y Sbjct: 599 VVLEGTGGIGKSTLINTLVGLDFFSDTHFDI---------GTGKDSYEQIAGIVAY---- 645 Query: 93 VRQNIAFPLTLAKMRKADIAQKVSETAKILDLTNLLDRKPSQLSGGQRQRVAMGRAIVRH 152 ++ + R+AD + + D R R A GR + H Sbjct: 646 ---ELS---EMTAFRRADAEAVKAFFSSRKD----------------RYRGAYGRYVQDH 683 Query: 153 PK 154 P+ Sbjct: 684 PR 685

Rv1301

Rv1308

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
Rv1301	-1	14	-3.003087	Conserved protein
Rv1302	0	14	-3.158500	Probable undecapaprenyl-phosphate
Rv1303	3	13	-3.697625	Conserved hypothetical transmembrane protein
Rv1304	3	12	-3.507763	Probable ATP synthase a chain AtpB (protein 6)
Rv1305	3	12	-2.704059	Probable ATP synthase C chain AtpE
Rv1306	4	11	-2.621392	Probable ATP synthase B chain AtpF
Rv1307	4	12	-2.401998	Probable ATP synthase delta chain AtpH
Rv1308	2	12	-1.805793	Probable ATP synthase alpha chain AtpA

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv1307	IGASERPTASE	32	0.006	IgA-specific serine endopeptidase (S6) signature.
>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature. Length = 1541 Score = 32.0 bits (72), Expect = 0.006 Identities = 23/117 (19%), Positives = 46/117 (39%), Gaps = 10/117 (8%) Query: 31 SARQDTVRQQLADAAAAADRLAEASQ----AHTKALEDAKSEAHRVVEEARTDAERIAEQ 86 S + Q + A +A+ ++ A+T+ E A+S + +E +T + Sbjct: 1048 SKTVEKNEQDATETTAQNREVAKEAKSNVKANTQTNEVAQSGSE--TKETQTTETKETAT 1105 Query: 87 LEAQADVEAERIKMQGARQVDLIRAQLT-RQLRLELGHESVRQARELVRNHVADQAQ 142 +E + + E K Q +V +Q++ +Q + E ARE + Q Sbjct: 1106 VEKEEKAKVETEKTQEVPKV---TSQVSPKQEQSETVQPQAEPARENDPTVNIKEPQ 1159

Rv1350

Rv1355c

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
Rv1350	-1	23	-4.364834	Probable 3-oxoacyl-[acyl-carrier protein]
Rv1351	0	25	-4.722541	Hypothetical protein
Rv1352	-1	23	-4.466915	Conserved protein
Rv1353c	0	21	-4.129498	Probable transcriptional regulatory protein
Rv1354c	0	22	-4.165777	Conserved hypothetical protein
Rv1355c	0	21	-3.923460	Possible molybdopterin biosynthesis protein

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv1350	DHBDHDRGNASE	114	5e-33	2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase signature. Length = 261 Score = 114 bits (287), Expect = 5e-33 Identities = 69/258 (26%), Positives = 120/258 (46%), Gaps = 19/258 (7%) Query: 2 ASLLNARTAVITGGAQGLGLAIGQRFVAEGARVVLGDVNLEATEVAAKRLGGDD-VALAV 60 A + + A ITG AQG+G A+ + ++GA + D N E E L + A A Sbjct: 3 AKGIEGKIAFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHAEAF 62 Query: 61 RCDVTQADDVDILIRTAVERFGGLDVMVNNAGITRDATMRTMTEEQFDQVIAVHLKGTWN 120 DV + +D + G +D++VN AG+ R + ++++E+++ +V+ G +N Sbjct: 63 PADVRDSAAIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGVFN 122 Query: 121 GTRLAAAIMRERKRGAIVNMSSVSGKVGMVGQTNYSAAKAGIVGMTKAAAKELAHLGIRV 180 +R + M +R+ G+IV + S V Y+++KA V TK ELA IR Sbjct: 123 ASRSVSKYMMDRRSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLELAEYNIRC 182 Query: 181 NAIAPGLIRSAMTEAMPQRIWDQKLAE--------------VPMGRAGEPSEVASVAVFL 226 N ++PG + M +W + +P+ + +PS++A +FL Sbjct: 183 NIVSPGSTETDMQ----WSLWADENGAEQVIKGSLETFKTGIPLKKLAKPSDIADAVLFL 238 Query: 227 ASDLSSYMTGTVLDVTGG 244 S + ++T L V GG Sbjct: 239 VSGQAGHITMHNLCVDGG 256
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv1353c	TETREPRESSOR	85	3e-22	Tetracycline repressor protein signature.
>TETREPRESSOR#Tetracycline repressor protein signature. Length = 218 Score = 85.4 bits (211), Expect = 3e-22 Identities = 51/207 (24%), Positives = 84/207 (40%), Gaps = 15/207 (7%) Query: 16 INPEDIISGAFELAQQVSIDNLSMPLLGKHLGVGVTSIYWYFRKKDDLLNAMTDRALSKY 75 +N E +I A EL + ID L+ L + LG+ ++YW+ + K LL+A+ L+++ Sbjct: 4 LNRESVIDAALELLNETGIDGLTTRKLAQKLGIEQPTLYWHVKNKRALLDALAVEILARH 63 Query: 76 VFATPYIEAGDWRETLRNHARSMRKTFADNPVLCDLILIRAALSPKTARLGAQEMEKAIA 135 + W+ LRN+A S R+ D + P + +E + Sbjct: 64 HDYSLPAAGESWQSFLRNNAMSFRRALL---RYRDGAKVHLGTRPDEKQYDT--VETQLR 118 Query: 136 NLVTAGLSLEDAFDIYSAVSVHVRGSVVLDRLSRKSQSAGSGPSAIEHPVAIDPATTPLL 195 + G SL D SAVS G+V+ + + + + P A D PLL Sbjct: 119 FMTENGFSLRDGLYAISAVSHFTLGAVLEQQEHTAALT--------DRPAAPDENLPPLL 170 Query: 196 AHATGRGHRIGAPDETNFEYGLECILD 222 A E F +GLE ++ Sbjct: 171 REALQIMDSDDG--EQAFLHGLESLIR 195

Rv1441c

Rv1455

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
Rv1441c	0	15	3.028606	PE-PGRS family protein PE_PGRS26
Rv1442	-2	12	0.660131	Probable biotin sulfoxide reductase BisC (BDS
Rv1443c	1	13	-0.150018	Unknown protein
Rv1444c	0	14	0.476997	Unknown protein
Rv1445c	2	18	5.099400	Probable 6-phosphogluconolactonase DevB (6PGL)
Rv1446c	2	17	4.892553	Putative OXPP cycle protein OpcA
Rv1447c	4	17	6.402025	Probable glucose-6-phosphate 1-dehydrogenase
Rv1448c	5	17	6.356595	Probable transaldolase Tal
Rv1449c	5	18	6.709170	Transketolase Tkt (TK)
Rv1450c	7	18	7.212018	PE-PGRS family protein PE_PGRS27
Rv1451	2	11	3.865455	Probable cytochrome C oxidase assembly factor
Rv1452c	2	13	3.941429	PE-PGRS family protein PE_PGRS28
Rv1453	-1	11	0.734909	Possible transcriptional activator protein
Rv1454c	1	11	1.342042	Probable quinone reductase Qor (NADPH:quinone
Rv1455	2	11	1.686353	Conserved protein

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv1441c	cloacin	37	2e-04	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 36.6 bits (84), Expect = 2e-04 Identities = 36/103 (34%), Positives = 41/103 (39%), Gaps = 1/103 (0%) Query: 300 GTAGQGGDGGTALGAGGIGGDGGTGGAGGTGGTAGIGGSSAGAGGAGGDGGAGGTGGGSS 359 G G+G + G +G I G G TG G G + G G SS GG G GGGS Sbjct: 3 GGDGRGHNTGAHSTSGNING-GPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 360 MIGGKGGTGGNGGVGGTGGASALTIGNGSSAGAGGAGGAGGTG 402 G G GG G G SA+ A GAGG Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 36.2 bits (83), Expect = 3e-04 Identities = 34/116 (29%), Positives = 38/116 (32%), Gaps = 2/116 (1%) Query: 233 GGGDAGGAAGMFGTGGA--GGTGGDGGAGGAGDSPNSGANGARGGDGGNGAAGGAGGRLF 290 GG G G T G GG G G GGA D + G G GG Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 291 GNGGAGGNGGTAGQGGDGGTALGAGGIGGDGGTGGAGGTGGTAGIGGSSAGAGGAG 346 GNGG GN G G +A+ A G G G I + A A Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAAIAD 118 Score = 35.1 bits (80), Expect = 6e-04 Identities = 28/81 (34%), Positives = 33/81 (40%), Gaps = 2/81 (2%) Query: 291 GNGGAGGNGGTAGQGGDGGTALGAGGIGGDGGTGGAGGTGGTAGIGGSSAGAGGAGGDGG 350 G G G N G G+ G+GG G + GGS +G GG G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 351 AGGTGGGSSMIGGKGGTGGNG 371 G G G+S GG GTGGN Sbjct: 63 GNGGGNGNS--GGGSGTGGNL 81 Score = 35.1 bits (80), Expect = 7e-04 Identities = 33/101 (32%), Positives = 43/101 (42%), Gaps = 1/101 (0%) Query: 369 GNGGVGGTGGASALTIGNGSSAGAGGAGGAGGTGGTGGYIESLDGKGQAGNGGNGGNGAA 428 G G G GA + T GN + G G G + G+G E+ G +G+G + G G+ Sbjct: 3 GGDGRGHNTGAHS-TSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 429 GGAGGGGTGAGGNGGAGGNGGDGGPSQGGGNPGFGGDGGTG 469 G GGG +GG G GGN G P G G Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG 102 Score = 34.7 bits (79), Expect = 7e-04 Identities = 29/116 (25%), Positives = 38/116 (32%) Query: 248 GAGGTGGDGGAGGAGDSPNSGANGARGGDGGNGAAGGAGGRLFGNGGAGGNGGTAGQGGD 307 G G G + GA + N G G G G + +G + GG+G G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 308 GGTALGAGGIGGDGGTGGAGGTGGTAGIGGSSAGAGGAGGDGGAGGTGGGSSMIGG 363 G GG G G G + GAGG + G S+ I Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAAIAD 118 Score = 33.9 bits (77), Expect = 0.002 Identities = 27/92 (29%), Positives = 35/92 (38%), Gaps = 4/92 (4%) Query: 390 AGAGGAGGAGGTGGTGGYIESLDGKGQAGNGGNGGNGAAG----GAGGGGTGAGGNGGAG 445 +G G G G T G I G G + G+G + GG G+G GG+G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 446 GNGGDGGPSQGGGNPGFGGDGGTGGPGGVGVP 477 G G + GGG+ G P G P Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFP 93 Score = 33.5 bits (76), Expect = 0.002 Identities = 28/85 (32%), Positives = 34/85 (40%), Gaps = 3/85 (3%) Query: 326 AGGTGGTAGIGGSSAGAGGAGGDGGAGGTGGGSSMIGGKGGTGGNGGVGGTGGASALTIG 385 +GG G G S GG G G GG S G G + N GG G+ G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASD---GSGWSSENNPWGGGSGSGIHWGG 58 Query: 386 NGSSAGAGGAGGAGGTGGTGGYIES 410 GG G +GG GTGG + + Sbjct: 59 GSGHGNGGGNGNSGGGSGTGGNLSA 83 Score = 33.5 bits (76), Expect = 0.002 Identities = 31/109 (28%), Positives = 37/109 (33%) Query: 258 AGGAGDSPNSGANGARGGDGGNGAAGGAGGRLFGNGGAGGNGGTAGQGGDGGTALGAGGI 317 +GG G N+GA+ G G G GG G G G G G G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 318 GGDGGTGGAGGTGGTAGIGGSSAGAGGAGGDGGAGGTGGGSSMIGGKGG 366 G+GG G G G G S+ A A G G G + G Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAG 110 Score = 33.1 bits (75), Expect = 0.002 Identities = 31/105 (29%), Positives = 37/105 (35%), Gaps = 2/105 (1%) Query: 363 GKGGTGGNGGVGGT--GGASALTIGNGSSAGAGGAGGAGGTGGTGGYIESLDGKGQAGNG 420 G+G G G GG + L +G G+S G+G + GG G G GNG Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNG 65 Query: 421 GNGGNGAAGGAGGGGTGAGGNGGAGGNGGDGGPSQGGGNPGFGGD 465 G GN G GG A A G P GG Sbjct: 66 GGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAG 110 Score = 33.1 bits (75), Expect = 0.003 Identities = 26/88 (29%), Positives = 34/88 (38%), Gaps = 5/88 (5%) Query: 229 GGNGGGGDAGGAAGMFGTGGAGGTGGDGGAG-GAGDSPNSGANGARGGDGGNGAAGGAGG 287 G N G G TG G G G+G + ++P G +G+ GG G GG Sbjct: 8 GHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGG 67 Query: 288 RLFGNGGAGGNGGTAGQGGDGGTALGAG 315 NG +GG GT G + G Sbjct: 68 ----NGNSGGGSGTGGNLSAVAAPVAFG 91 Score = 32.4 bits (73), Expect = 0.005 Identities = 26/79 (32%), Positives = 30/79 (37%), Gaps = 4/79 (5%) Query: 412 DGKGQAGNGGNGGNGAAGGAGGGGTGAGGNGGAG----GNGGDGGPSQGGGNPGFGGDGG 467 DG+G + GG G G G G + G+G N GG G G G G Sbjct: 5 DGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGN 64 Query: 468 TGGPGGVGVPDGIGGANGA 486 GG G G G GG A Sbjct: 65 GGGNGNSGGGSGTGGNLSA 83 Score = 31.6 bits (71), Expect = 0.007 Identities = 27/88 (30%), Positives = 37/88 (42%), Gaps = 2/88 (2%) Query: 198 LSGNGGAGGNGGTGASGADGGGGLPPVPASPGGNGGGGDAGGAAGMFGTGGAGGTGGDGG 257 +SG G G N G ++ + GG P GG G + GG+G GG Sbjct: 1 MSGGDGRGHNTGAHSTSGNINGG--PTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGG 58 Query: 258 AGGAGDSPNSGANGARGGDGGNGAAGGA 285 G G+ +G +G G GGN +A A Sbjct: 59 GSGHGNGGGNGNSGGGSGTGGNLSAVAA 86 Score = 30.5 bits (68), Expect = 0.016 Identities = 27/78 (34%), Positives = 32/78 (41%), Gaps = 3/78 (3%) Query: 142 GNGGNGGAGDAAHPNGGNGGDAGMFGNGGAG-GAGYSPAAGTGAAGGAGGAGGAGGWLSG 200 G G N GA + NGG G+ GGA G+G+S G G GG G Sbjct: 6 GRGHNTGAHSTS--GNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHG 63 Query: 201 NGGAGGNGGTGASGADGG 218 NGG GN G G+ Sbjct: 64 NGGGNGNSGGGSGTGGNL 81 Score = 30.1 bits (67), Expect = 0.021 Identities = 34/111 (30%), Positives = 37/111 (33%), Gaps = 11/111 (9%) Query: 161 GDAGMFGNGGAGGAGYSPAAGTGAAGGAGGAGGAGGWLSGNGGAGGNGGTGASGADGGGG 220 G G N GA + G G GGA GW S N GG G+G G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 221 LPPVPASPGGNGGGGDAGGAAGMFGTGGAGGTGGDGGAGGAGDSPNSGANG 271 G GG G + G GTGG A G GA G Sbjct: 63 -----------GNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG 102 Score = 28.9 bits (64), Expect = 0.047 Identities = 28/73 (38%), Positives = 32/73 (43%), Gaps = 4/73 (5%) Query: 422 NGGNGAAGGAGGGGTGAGGNGGAGGNGGDGGPSQGGG----NPGFGGDGGTGGPGGVGVP 477 +GG+G G T NGG G G GG S G G N +GG G+G G G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 478 DGIGGANGAQGKH 490 G GG NG G Sbjct: 62 HGNGGGNGNSGGG 74
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv1450c	cloacin	41	2e-05	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 41.2 bits (96), Expect = 2e-05 Identities = 35/100 (35%), Positives = 39/100 (39%), Gaps = 2/100 (2%) Query: 839 GDGGNGGNGGSAGTGGN--GGRGGDGAFGGMSANATNPGENGPNGNPGGNGGAGGAGGAG 896 G G G N G+ T GN GG G G GG S + EN P G G+G G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 897 LNGGNGGAGGNGGLGGFGGNGAAGANGVAVGAPGQPGGAG 936 NGG G G G G + A A PG G Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG 102 Score = 40.1 bits (93), Expect = 6e-05 Identities = 32/103 (31%), Positives = 36/103 (34%) Query: 408 SSGGKAGGNGGAGGAGGLVGNGGAGGAGGNGAPGAPPSGGDPNGGGGGAGGAGGKGGDGG 467 S G G N GA G + G G G GA + N GGG+G GG G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 468 AQAGDGGAGGAGGKGGNGGNGATGATGLNGLGAGADGTDGGKG 510 G G GG G G A A G A + GG Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 38.5 bits (89), Expect = 2e-04 Identities = 38/111 (34%), Positives = 46/111 (41%), Gaps = 3/111 (2%) Query: 394 SGGNGGAGGNGAHFSSGGKAGGNGGAGGAGGLVGNGGAGGAGGNGAPGAPPSGGDPNGGG 453 SGG+G GAH +SG GG G G GG + G+G + N P SG + GG Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGG--ASDGSGWSSENN-PWGGGSGSGIHWGG 58 Query: 454 GGAGGAGGKGGDGGAQAGDGGAGGAGGKGGNGGNGATGATGLNGLGAGADG 504 G G GG G+ G +G GG A G A G GL Sbjct: 59 GSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISA 109 Score = 38.5 bits (89), Expect = 2e-04 Identities = 36/102 (35%), Positives = 45/102 (44%), Gaps = 3/102 (2%) Query: 780 GNGGHGGNGGNPGAGGQ--GGSGGAGSTPGAKGAHGFTPTSGGDGGDGGNGGNSQVVGGN 837 G G G N G G GG G G GA G++ + GG G+G + G+ Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 838 GGDGGNGGNGGSAGTGGNGGRGGDGAFGGMSANATNPGENGP 879 G GGNG +GG +GTGGN G A +T PG G Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALST-PGAGGL 103 Score = 37.4 bits (86), Expect = 4e-04 Identities = 31/79 (39%), Positives = 37/79 (46%), Gaps = 2/79 (2%) Query: 991 GGGGRGGDGGNAGNAGA--GGPGGTGSTAGKAGPAGSILHDGGNGGHGGHGAASGGNGGP 1048 GG GRG + G +G GGP G G G + +G + GG G G GG G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 1049 GGHGGNGGNGGTGANGGNG 1067 G GGNG +GG GGN Sbjct: 63 GNGGGNGNSGGGSGTGGNL 81 Score = 37.4 bits (86), Expect = 4e-04 Identities = 26/87 (29%), Positives = 31/87 (35%) Query: 934 GAGGHGGAGGNGGAGGNGGQGVVSDGAGGAGGAGGDGGAPGDGANGGNGQGAGAFAGGGG 993 G G G G GN G G GG G + + GG+G G G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 994 GRGGDGGNAGNAGAGGPGGTGSTAGKA 1020 G GG GN+G G + A A Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVA 89 Score = 37.4 bits (86), Expect = 4e-04 Identities = 34/92 (36%), Positives = 39/92 (42%), Gaps = 9/92 (9%) Query: 971 GAPGDGANGGNGQGAGAFAGGGGGRGGDGGNAGNAGAGGPGGTGSTAGKAGPAGSILHDG 1030 G G G N G +G GG G G GG + G G + G +GS +H G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGG-----ASDGSGWSSENNPWGGGSGSGIHWG 57 Query: 1031 GNGGHGGHGAASGGNGGPGGHGGNGGNGGTGA 1062 G GHG GGNG GG G GGN A Sbjct: 58 GGSGHGN----GGGNGNSGGGSGTGGNLSAVA 85 Score = 37.4 bits (86), Expect = 4e-04 Identities = 36/108 (33%), Positives = 41/108 (37%), Gaps = 5/108 (4%) Query: 1176 TGGTGGTGGTGGQGANGGLTGGRGGTGGNGGNGNTGGTGGAGGTGGTGHNGSQPGMGGNG 1235 +GG G TG +G + GG G G GG + G G G GS GG Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGS-GSGIHWGGGS 60 Query: 1236 GAGGFGGNGFAGVGGRGGMGGSGGTGGTGDAGPFGTGTGGTGGHGGQG 1283 G G GGNG +G GG G G FG T G GG Sbjct: 61 GHGNGGGNGNSG----GGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 36.6 bits (84), Expect = 8e-04 Identities = 30/87 (34%), Positives = 37/87 (42%) Query: 728 GNGGQGGPGGLAGNLFGQNGIQGVGGSGGKGGAGGLAGDGGNGANGNFAFGDGNGGHGGN 787 G G G +GN+ G GVGG G + G +G+ G GHG Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNG 65 Query: 788 GGNPGAGGQGGSGGAGSTPGAKGAHGF 814 GGN +GG G+GG S A A GF Sbjct: 66 GGNGNSGGGSGTGGNLSAVAAPVAFGF 92 Score = 36.2 bits (83), Expect = 8e-04 Identities = 36/102 (35%), Positives = 43/102 (42%), Gaps = 7/102 (6%) Query: 361 GDNGGDPGAGGA--GGTGGAGSTIGAHGAAGASPTSGGNGGAGGNGAHFSSGGKAGGNGG 418 G N G G GG G G GA +G S + GG G+G H+ G G GG Sbjct: 8 GHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGG 67 Query: 419 AGGAGGLVGNGGAGGAGGNGAPGAPPSGGDPNGGGGGAGGAG 460 G +G GG+G G A AP + G P GAGG Sbjct: 68 NGNSG-----GGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 35.5 bits (81), Expect = 0.001 Identities = 31/83 (37%), Positives = 39/83 (46%), Gaps = 3/83 (3%) Query: 1145 GGGGRGGDAGRGGDAGLGGSSGPGGTPGDWGTGGTGGTGGTGGQGANGGLTGGRGGTGGN 1204 GG GRG + G G+ G T G G + G+G + GG +G GG Sbjct: 3 GGDGRGHN---TGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGG 59 Query: 1205 GGNGNTGGTGGAGGTGGTGHNGS 1227 G+GN GG G +GG GTG N S Sbjct: 60 SGHGNGGGNGNSGGGSGTGGNLS 82 Score = 35.5 bits (81), Expect = 0.002 Identities = 30/87 (34%), Positives = 36/87 (41%), Gaps = 1/87 (1%) Query: 445 SGGDPNGGGGGAGGAGGKGGDGGAQAGDGGAGGAGGKGGNGGNGATGATGLNGLGAGADG 504 SGGD G GA G +GG G G + G G + N G +G+ G Sbjct: 2 SGGDGRGHNTGAHSTSGN-INGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGS 60 Query: 505 TDGGKGGNGGAGGGGGAGGQGGKALAA 531 G GGNG +GGG G GG A Sbjct: 61 GHGNGGGNGNSGGGSGTGGNLSAVAAP 87 Score = 35.1 bits (80), Expect = 0.002 Identities = 32/110 (29%), Positives = 40/110 (36%) Query: 521 AGGQGGKALAATHQDGSMGAGGAGGNGGAGGMGGDGGNGAKGTFDNGGDGVGGNGGNGGS 580 +GG G H GG G G GG G ++ GG G G + G G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 581 RGIGGAGGIGGAGSTAGADGARGATPTSGGNGGTGGNGANATVAGGAGGA 630 G GG G G GS G + + A P + G GA + GA Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGA 111 Score = 35.1 bits (80), Expect = 0.002 Identities = 32/93 (34%), Positives = 37/93 (39%), Gaps = 8/93 (8%) Query: 1042 SGGNGGPGGHGGNGGNGGTGANGGNGGIGGTGGAGSTGAKGVLGTNEGDGGDGGRGGNGG 1101 SGG+G GH + NGG G+G GGA G G G GG Sbjct: 2 SGGDGR--GHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGG 59 Query: 1102 RGGNGGQGLTGAGGNGGTGGTPGNGGNGGNGAS 1134 G G GGNG +GG G GGN A+ Sbjct: 60 SGHGNG------GGNGNSGGGSGTGGNLSAVAA 86 Score = 34.7 bits (79), Expect = 0.003 Identities = 29/81 (35%), Positives = 38/81 (46%), Gaps = 4/81 (4%) Query: 484 NGGNGATGATGLNGLGAGADGTDGGKGGNGGAGGGGGAGGQ----GGKALAATHQDGSMG 539 +GG+G TG + +G G G GGA G G + GG + + H G G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 540 AGGAGGNGGAGGMGGDGGNGA 560 G GGNG +GG G GGN + Sbjct: 62 HGNGGGNGNSGGGSGTGGNLS 82 Score = 33.9 bits (77), Expect = 0.005 Identities = 30/89 (33%), Positives = 38/89 (42%), Gaps = 1/89 (1%) Query: 566 NGGDGVGGNGGNGGSRGIGGAGGIGGAGSTAGADGARGATPTSGGNGGTGGNGANATVAG 625 +GGDG G N G + G GG G G GA G + + GG G+G + Sbjct: 2 SGGDGRGHNTGAHSTSG-NINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGS 60 Query: 626 GAGGAGGKGGNGGLVGNGGAGGKGGDGMA 654 G G GG G +GG G GG +A Sbjct: 61 GHGNGGGNGNSGGGSGTGGNLSAVAAPVA 89 Score = 33.9 bits (77), Expect = 0.005 Identities = 30/89 (33%), Positives = 39/89 (43%), Gaps = 2/89 (2%) Query: 629 GAGGKGGNGGLVGNGG--AGGKGGDGMAGVAGSSPTTAGESGTSGQNGGAGGAGGAGGRG 686 G G+G N G G GG G G+ G A + E+ G G+G G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 687 GDFGGDGGTGGAGGNGANGANATTPGAKG 715 G+ GG+G +GG G G N + P A G Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFG 91 Score = 32.8 bits (74), Expect = 0.010 Identities = 26/71 (36%), Positives = 29/71 (40%) Query: 884 GGNGGAGGAGGAGLNGGNGGAGGNGGLGGFGGNGAAGANGVAVGAPGQPGGAGGHGGAGG 943 G N GA G G G G G G G + G G+ GG GHG GG Sbjct: 8 GHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGG 67 Query: 944 NGGAGGNGGQG 954 NG +GG G G Sbjct: 68 NGNSGGGSGTG 78 Score = 32.4 bits (73), Expect = 0.012 Identities = 35/115 (30%), Positives = 45/115 (39%), Gaps = 1/115 (0%) Query: 1029 DGGNGGHGGHGAASGGNGGPGGHGGNGGNGGTGANGGNGGIGGTGGAGSTGAKGVLGTNE 1088 DG G H + NGGP G G GG G+ + GG+GS G + Sbjct: 5 DGRGHNTGAHSTSGNINGGPTGLGVGGG-ASDGSGWSSENNPWGGGSGSGIHWGGGSGHG 63 Query: 1089 GDGGDGGRGGNGGRGGNGGQGLTGAGGNGGTGGTPGNGGNGGNGASGDLVTSPGD 1143 GG+G GG G GGN TPG GG + ++G L + D Sbjct: 64 NGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAAIAD 118 Score = 32.4 bits (73), Expect = 0.014 Identities = 29/79 (36%), Positives = 33/79 (41%), Gaps = 2/79 (2%) Query: 168 GTGGAGGAGGAGAAGGA--GGSGGWLLGNGGVGGAGGQSLLGGATGGAGGNAGLFGVGGT 225 G G G GA + G GG G +G G G+G S GG+G G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 226 GGPGGPGGPGGVGGTGGAG 244 G GG G GG GTGG Sbjct: 63 GNGGGNGNSGGGSGTGGNL 81 Score = 32.0 bits (72), Expect = 0.021 Identities = 29/97 (29%), Positives = 34/97 (35%), Gaps = 2/97 (2%) Query: 1142 GDGGGGGRGGDAGRGGDAGLGGSSGPGGTPGDWGTGGTGGTGGTGGQGANGGLTGGRGGT 1201 G G GG GLG G G W + GG+G G + G G G Sbjct: 8 GHNTGAHSTSGNINGGPTGLGVGGGASDGSG-WSSENNPWGGGSG-SGIHWGGGSGHGNG 65 Query: 1202 GGNGGNGNTGGTGGAGGTGGTGHNGSQPGMGGNGGAG 1238 GGNG +G GTGG P + G G Sbjct: 66 GGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG 102 Score = 31.2 bits (70), Expect = 0.029 Identities = 29/104 (27%), Positives = 35/104 (33%), Gaps = 15/104 (14%) Query: 859 GGDGAFGGMSANATNPGENGPNGNPGGNGGAGGAGGAGLNGGNGGAGGNGGLGGFGGNGA 918 GGDG A++T+ NG G GGA G G G G+ GG+G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG- 61 Query: 919 AGANGVAVGAPGQPGGAGGHGGAGGNGGAGGNGGQGVVSDGAGG 962 G GG G G G G V + A G Sbjct: 62 --------------HGNGGGNGNSGGGSGTGGNLSAVAAPVAFG 91 Score = 31.2 bits (70), Expect = 0.032 Identities = 31/83 (37%), Positives = 36/83 (43%), Gaps = 4/83 (4%) Query: 1098 GNGGRGGNGGQGLTGAGGNGGTGGTPGNGGNGGNGASGDLVTSPGDGGGGGRGGDAGRGG 1157 G GRG N G T NGG G G G + SG +S + GGG G GG Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTG--LGVGGGASDGSG--WSSENNPWGGGSGSGIHWGG 58 Query: 1158 DAGLGGSSGPGGTPGDWGTGGTG 1180 +G G G G + G GTGG Sbjct: 59 GSGHGNGGGNGNSGGGSGTGGNL 81 Score = 30.8 bits (69), Expect = 0.040 Identities = 31/99 (31%), Positives = 35/99 (35%), Gaps = 2/99 (2%) Query: 1070 GGTGGAGSTGAKGVLGTNEGDGGDGGRGGNGGRGGNGGQGLTGAGGNGGTGGTPGNGGNG 1129 GG G +TGA G +GG G G GG G GG+G GG Sbjct: 3 GGDGRGHNTGAHSTSGNI--NGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGS 60 Query: 1130 GNGASGDLVTSPGDGGGGGRGGDAGRGGDAGLGGSSGPG 1168 G+G G S G G GG G S PG Sbjct: 61 GHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPG 99
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv1452c	cloacin	38	2e-04	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 37.8 bits (87), Expect = 2e-04 Identities = 31/81 (38%), Positives = 39/81 (48%), Gaps = 1/81 (1%) Query: 463 SGGNGGAGGNGANATTAGTNGANGGPGGHGGLV-GNGGAGGNGANGAAGTNASDSGAVGG 521 SGG+G GA++T+ NG G G GG G+G + N G + G G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 522 KGNSGGNGGQGGAGGDGGTLA 542 GN GGNG GG G GG L+ Sbjct: 62 HGNGGGNGNSGGGSGTGGNLS 82 Score = 33.9 bits (77), Expect = 0.002 Identities = 29/79 (36%), Positives = 33/79 (41%), Gaps = 2/79 (2%) Query: 168 GTGGAGGAGGAGAAGGA--GGSGGWLLGNGGVGGAGGQSLLGGATGGAGGNAGLFGVGGT 225 G G G GA + G GG G +G G G+G S GG+G G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 226 GGPGGPGGPGGVGGTGGAG 244 G GG G GG GTGG Sbjct: 63 GNGGGNGNSGGGSGTGGNL 81 Score = 32.4 bits (73), Expect = 0.008 Identities = 25/86 (29%), Positives = 34/86 (39%), Gaps = 2/86 (2%) Query: 433 GGDPGAGGAGGTGGAGSITGAQGAIGATPTSGGNGGAGGNGANATTAGTNGANGGPGGHG 492 GGD G +G+I G +G G + G+G + N G +G+ GG Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLG--VGGGASDGSGWSSENNPWGGGSGSGIHWGGGS 60 Query: 493 GLVGNGGAGGNGANGAAGTNASDSGA 518 G GG G +G G N S A Sbjct: 61 GHGNGGGNGNSGGGSGTGGNLSAVAA 86 Score = 32.0 bits (72), Expect = 0.010 Identities = 26/80 (32%), Positives = 30/80 (37%), Gaps = 4/80 (5%) Query: 527 GNGGQGGAGGDGGTLAGNGGAGGTGGRGADGGLGGSGAEGANATTAGERGQDGGKGGNGG 586 G G GA G + G G GG +DG SG N G G GG G Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGGASDG----SGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 587 VGGTGGNAVAPGANGGHGGN 606 G GGN + G +G G Sbjct: 62 HGNGGGNGNSGGGSGTGGNL 81 Score = 30.8 bits (69), Expect = 0.022 Identities = 30/106 (28%), Positives = 36/106 (33%), Gaps = 5/106 (4%) Query: 127 AGAPGTGQAGGAGGILWGNGGAGGSGAPGQVGGAGGAAGLFGTGGAGGAGGAGAAGGAGG 186 +G G G GA + +G P +G GGA+ G G G+ G Sbjct: 2 SGGDGRGHNTGAH-----STSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHW 56 Query: 187 SGGWLLGNGGVGGAGGQSLLGGATGGAGGNAGLFGVGGTGGPGGPG 232 GG GNGG G G G A FG PG G Sbjct: 57 GGGSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG 102 Score = 30.5 bits (68), Expect = 0.029 Identities = 30/94 (31%), Positives = 34/94 (36%), Gaps = 3/94 (3%) Query: 372 MGGAGGAGGPGGAGGLISLLGGQGAGGAGGTGGAGGVGGDRGAGGPGNQAFNAGAGGAGG 431 M G G G GA + G G G G GGA G P +G GG Sbjct: 1 MSGGDGRGHNTGAHSTSGNING-GPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGG 59 Query: 432 HGGDPGAGGAGGTGGAGSITGAQGAIGATPTSGG 465 G GG G G GS TG + A P + G Sbjct: 60 SGHGN--GGGNGNSGGGSGTGGNLSAVAAPVAFG 91 Score = 30.1 bits (67), Expect = 0.039 Identities = 28/102 (27%), Positives = 35/102 (34%), Gaps = 5/102 (4%) Query: 302 GEDLSPHGTSGGVGGDAGDGGTGGRGGWLAGAGGAGGAGGVGGTGGAGGAGFSRALIVAG 361 G + H TSG + G G GG + G+G + GG G+G Sbjct: 8 GHNTGAHSTSGNINGGPTGLGVGG-----GASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 362 DNGGDGGNGGMGGAGGAGGPGGAGGLISLLGGQGAGGAGGTG 403 NGG GN G G G A + GAGG Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104

Rv1497

Rv1521

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
Rv1497	2	21	-5.453310	Probable esterase LipL
Rv1498c	4	29	-8.700460	Probable methyltransferase
Rv1498A	4	32	-8.637430	Conserved protein
Rv1499	3	30	-8.418661	Hypothetical protein
Rv1500	3	31	-9.589654	Probable glycosyltransferase
Rv1501	3	32	-9.483078	Conserved hypothetical protein
Rv1502	4	32	-9.864424	Hypothetical protein
Rv1503c	4	33	-9.981331	Conserved hypothetical protein
Rv1504c	1	28	-7.726387	Conserved hypothetical protein
Rv1505c	2	31	-8.016349	Conserved hypothetical protein
Rv1506c	1	30	-7.721838	Hypothetical protein
Rv1507c	1	25	-4.930333	Conserved protein
Rv1507A	0	18	-3.403531	Hypothetical protein
Rv1508c	-1	13	-2.071386	Probable membrane protein
Rv1508A	0	14	-1.944980	Conserved hypothetical protein
Rv1509	0	13	-1.864829	Hypothetical protein
Rv1510	1	13	-1.525100	Conserved probable membrane protein
Rv1511	2	11	-2.163516	GDP-D-mannose dehydratase GmdA (GDP-mannose 4,6
Rv1512	1	13	-2.000185	Probable nucleotide-sugar epimerase EpiA
Rv1513	0	13	-2.819922	Conserved protein
Rv1514c	-1	13	-2.747101	Conserved hypothetical protein
Rv1515c	-1	13	-2.588083	Conserved hypothetical protein
Rv1516c	-1	13	-2.972502	Probable sugar transferase
Rv1517	-1	11	-3.896901	Conserved hypothetical transmembrane protein
Rv1518	0	11	-3.873167	Conserved hypothetical protein
Rv1519	0	12	-3.517979	Conserved hypothetical protein
Rv1520	0	12	-3.538137	Probable sugar transferase
Rv1521	0	13	-3.251841	Probable fatty-acid-AMP ligase FadD25

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv1511	NUCEPIMERASE	94	4e-24	Nucleotide sugar epimerase signature.
>NUCEPIMERASE#Nucleotide sugar epimerase signature. Length = 334 Score = 94.5 bits (235), Expect = 4e-24 Identities = 70/337 (20%), Positives = 122/337 (36%), Gaps = 48/337 (14%) Query: 3 RALITGITGQDGSYLAELLLAKGYEVHGLIRRASTFNTSRIDHLYVDPHQPGARL----- 57 + L+TG G G ++++ LL G++V G+ N Y D ARL Sbjct: 2 KYLVTGAAGFIGFHVSKRLLEAGHQVVGI----DNLND------YYDVSLKQARLELLAQ 51 Query: 58 ---FLHYGDLIDGTRLVTLLSTIEPDEVYNLAAQSHVRVSFDEPVHTGDTTGMGSMRLLE 114 H DL D + L ++ + V+ + VR S + P D+ G + +LE Sbjct: 52 PGFQFHKIDLADREGMTDLFASGHFERVFISPHRLAVRYSLENPHAYADSNLTGFLNILE 111 Query: 115 AVRLSRVHCRFYQASSSEMFGASP--PPQNELTPFYPRSPYGAAKVYSYWATRNYREAYG 172 R +++ ASSS ++G + P + + +P S Y A K + Y YG Sbjct: 112 GCRHNKIQ-HLLYASSSSVYGLNRKMPFSTDDSVDHPVSLYAATKKANELMAHTYSHLYG 170 Query: 173 LFAVNGILFNHESPRRGETFVTRKITRAVARIKAGIQSEVYMGNLDAVRDWGYAPEYVEG 232 L A F P K T+A + G +VY RD+ Y + E Sbjct: 171 LPATGLRFFTVYGPWGRPDMALFKFTKA---MLEGKSIDVY-NYGKMKRDFTYIDDIAEA 226 Query: 233 MWRMLQTDEPDD-------------------FVLATGRGFTVREFARAAFEHAGLDWQQY 273 + R+ D + + + ++ +A + G++ Sbjct: 227 IIRLQDVIPHADTQWTVETGTPAASIAPYRVYNIGNSSPVELMDYIQALEDALGIE---- 282 Query: 274 VKFDQRYLRPTEVDSLIGDATKAAELLGWRASVHTDE 310 K + L+P +V D E++G+ + Sbjct: 283 AKKNMLPLQPGDVLETSADTKALYEVIGFTPETTVKD 319
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv1512	NUCEPIMERASE	76	1e-17	Nucleotide sugar epimerase signature.
>NUCEPIMERASE#Nucleotide sugar epimerase signature. Length = 334 Score = 75.6 bits (186), Expect = 1e-17 Identities = 60/288 (20%), Positives = 104/288 (36%), Gaps = 35/288 (12%) Query: 48 ELDLTDRAATFDFVLESRPQVVIDAAARVGGILANDTYPADFLSENLQIQVNLLDAAVAA 107 ++DL DR D + V + R + + P + NL +N+L+ Sbjct: 58 KIDLADREGMTDLFASGHFERVFISPHR-LAVRYSLENPHAYADSNLTGFLNILEGCRHN 116 Query: 108 RVPRLLFLGSSCIYPKLAPQPIPESALLTGPLEPTNDAYAIAKIAGILAVQAVRRQHGLP 167 ++ LL+ SS +Y P + P+ YA K A L +GLP Sbjct: 117 KIQHLLYASSSSVYGLNRKMPFSTDDSVDHPVSL----YAATKKANELMAHTYSHLYGLP 172 Query: 168 WISAMPTNLYGP-GDNFSPSGSHLLPALIRRYDEAKASGAPNVTNWGTGTPRRELLHVDD 226 +YGP G P + L + E K+ +V + G +R+ ++DD Sbjct: 173 ATGLRFFTVYGPWGR---PDMA--LFKFTKAMLEGKS---IDV--YNYGKMKRDFTYIDD 222 Query: 227 LASACLYLLEHFDGPTH------------------VNVGTGIDHTIGEIAEMVASAVGYS 268 +A A + L + N+G + + + + A+G Sbjct: 223 IAEAIIRLQDVIPHADTQWTVETGTPAASIAPYRVYNIGNSSPVELMDYIQALEDALGIE 282 Query: 269 GETRWDPSKPDGTPRKLLDVSVLREA-GWRPSIALRDGIEATVAWYRE 315 + P +P D L E G+ P ++DG++ V WYR+ Sbjct: 283 AKKNMLPLQPGDVLETSADTKALYEVIGFTPETTVKDGVKNFVNWYRD 330

Rv1566c

Rv1587c

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
Rv1566c	2	14	4.344027	Possible Inv protein
Rv1567c	4	15	4.319819	Probable hypothetical membrane protein
Rv1568	3	16	5.097595	Adenosylmethionine-8-amino-7-oxononanoate
Rv1569	1	14	4.668894	Probable 8-amino-7-oxononanoate synthase BioF1
Rv1570	1	16	2.515554	Dethiobiotin synthetase BioD
Rv1571	5	17	0.922850	Conserved protein
Rv1572c	8	21	-0.272144	Conserved hypothetical protein
Rv1573	9	21	0.411396	Probable PhiRv1 phage protein
Rv1574	8	22	-0.362339	Probable PhiRv1 phage related protein
Rv1575	7	20	-0.174683	Probable PhiRv1 phage protein
Rv1576c	5	18	0.120555	Probable PhiRv1 phage protein
Rv1577c	2	19	-1.653645	Probable PhiRv1 phage protein
Rv1578c	0	18	-0.545462	Probable PhiRv1 phage protein
Rv1579c	1	20	-0.918660	Probable PhiRv1 phage protein
Rv1580c	0	19	-0.596089	Probable PhiRv1 phage protein
Rv1581c	-1	22	0.127174	Probable PhiRv1 phage protein
Rv1582c	-1	23	0.386844	Probable PhiRv1 phage protein
Rv1583c	0	24	1.646716	Probable PhiRv1 phage protein
Rv1584c	1	23	1.139208	Possible PhiRv1 phage protein
Rv1585c	2	22	1.137847	Possible phage PhiRv1 protein
Rv1586c	2	19	1.245997	Probable PhiRv1 integrase
Rv1587c	2	14	0.668879	Partial REP13E12 repeat protein

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv1573	PF07201	27	0.024	Hypersensitivity response secretion protein HrpJ
>PF07201#Hypersensitivity response secretion protein HrpJ Length = 293 Score = 27.1 bits (60), Expect = 0.024 Identities = 16/72 (22%), Positives = 22/72 (30%), Gaps = 12/72 (16%) Query: 44 EVREALVRLQAALNRHEHTGELEAFLRISVEHADAAGGDECGPAILAG-----------R 92 E E L + + EL L VE A + +E G I+ G + Sbjct: 131 EPSEQFKMLCGLRDALKGRPELAH-LSHLVEQALVSMAEEQGETIVLGARITPEAYRESQ 189 Query: 93 SGPEQAAINRQL 104 SG R Sbjct: 190 SGVNPLQPLRDT 201

Rv1646

Rv1667c

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
Rv1646	2	11	2.683243	PE family protein PE17
Rv1647	1	10	3.148189	Adenylate cyclase (ATP pyrophosphate-lyase)
Rv1648	0	10	3.632890	Probable transmembrane protein
Rv1649	0	10	3.753983	Probable phenylalanyl-tRNA synthetase, alpha
Rv1650	0	12	4.367663	Probable phenylalanyl-tRNA synthetase, beta
Rv1651c	1	15	4.602736	PE-PGRS family protein PE_PGRS30
Rv1652	1	22	4.477058	Probable N-acetyl-gamma-glutamyl-phoshate
Rv1653	2	25	4.087697	Probable glutamate N-acetyltransferase ArgJ
Rv1654	1	26	4.094706	Probable acetylglutamate kinase ArgB
Rv1655	1	23	3.747976	Probable acetylornithine aminotransferase ArgD
Rv1656	0	19	2.257773	Probable ornithine carbamoyltransferase,
Rv1657	3	25	1.179279	Probable arginine repressor ArgR (AHRC)
Rv1658	3	26	0.616814	Probable argininosuccinate synthase ArgG
Rv1659	3	28	0.072792	Probable argininosuccinate lyase ArgH
Rv1660	3	29	-0.366015	Chalcone synthase Pks10
Rv1661	3	28	-0.406753	Probable polyketide synthase Pks7
Rv1662	1	25	-1.338546	Probable polyketide synthase Pks8
Rv1663	-2	15	-1.002425	Probable polyketide synthase Pks17
Rv1664	-2	14	-0.820935	Probable polyketide synthase Pks9
Rv1665	0	13	-0.284414	Chalcone synthase Pks11
Rv1666c	1	14	-0.549422	Probable cytochrome P450 139 Cyp139
Rv1667c	2	14	0.056764	Probable second part of macrolide-transport

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv1649	PF07201	33	0.001	Hypersensitivity response secretion protein HrpJ
>PF07201#Hypersensitivity response secretion protein HrpJ Length = 293 Score = 32.9 bits (75), Expect = 0.001 Identities = 22/82 (26%), Positives = 31/82 (37%), Gaps = 9/82 (10%) Query: 12 DAAQQAIALADTLDVLA-RVKTEHLGDRSPLALARQALAVLPKEQ--RAEAGKRVNAARN 68 + ++Q L D L R + HL L QAL + +EQ G R+ Sbjct: 131 EPSEQFKMLCGLRDALKGRPELAHL-----SHLVEQALVSMAEEQGETIVLGARITPEAY 185 Query: 69 AAQRSYDERLATLR-AERDAAV 89 +S L LR RDA + Sbjct: 186 RESQSGVNPLQPLRDTYRDAVM 207
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv1651c	cloacin	45	1e-06	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 45.1 bits (106), Expect = 1e-06 Identities = 38/108 (35%), Positives = 43/108 (39%), Gaps = 5/108 (4%) Query: 605 SGGNGIGGKGGASGNGGNAGQVFGDGGTGGTGGAGGAGSGTKAGGTGSDGGHGGNATLIG 664 SGG+G G GA GN + G G GG GSG + GG G G Sbjct: 2 SGGDGRGHNTGAHSTSGN---INGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGG 58 Query: 665 NGGDGGAGGAGGAGSPAGAPGNGGTGGTGGVLFGQSGSSGPPGAAALA 712 G G GG G +G +G GN V FG S PGA LA Sbjct: 59 GSGHGNGGGNGNSGGGSGTGGNLSAVAA-PVAFGFPALS-TPGAGGLA 104 Score = 42.4 bits (99), Expect = 8e-06 Identities = 35/99 (35%), Positives = 40/99 (40%), Gaps = 6/99 (6%) Query: 119 GNGADGVAGTGSNAGGNGGPGGILYGNGGNGGAGGNGGAAGLIGNGGAGGAGGAGGAGGA 178 GN G G G G + G G N GG+G I GG G G GG G + Sbjct: 18 GNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSG------IHWGGGSGHGNGGGNGNS 71 Query: 179 GGAGGTGGLLYGNGGAGGNGGSAAAAGGAGGNALLFGNG 217 GG GTGG L G A + GAGG A+ G Sbjct: 72 GGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAG 110 Score = 42.4 bits (99), Expect = 8e-06 Identities = 41/116 (35%), Positives = 45/116 (38%), Gaps = 10/116 (8%) Query: 124 GVAGTGSNAGGNGGPGGILYGNGGNGGAGGNGGAAGLIG-------NGGAGGAGGAGGAG 176 G G G N G + G I NGG G G GGA+ G GG G+G G G Sbjct: 3 GGDGRGHNTGAHSTSGNI---NGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGG 59 Query: 177 GAGGAGGTGGLLYGNGGAGGNGGSAAAAGGAGGNALLFGNGGNGGSGASGGAAGHA 232 G GG G G G GGN + AA G AL G S GA A Sbjct: 60 SGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAA 115 Score = 40.9 bits (95), Expect = 3e-05 Identities = 35/99 (35%), Positives = 43/99 (43%), Gaps = 4/99 (4%) Query: 215 GNGGNGGSGASGGAAGHAGTIFGNGGNAGAGSGLAGAD----GGLFGNGGDGGSSTSKAG 270 G G N G+ ++ G T G GG A GSG + + GG GG S G Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNG 65 Query: 271 GAGGNALFGNGGDGGSSTVAAGGAGGNTLVGNGGAGGAG 309 G GN+ G+G G S VAA A G + GAGG Sbjct: 66 GGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 39.7 bits (92), Expect = 5e-05 Identities = 34/103 (33%), Positives = 40/103 (38%), Gaps = 6/103 (5%) Query: 147 GNGGAGGNGGAAGLIGN--GGAGGAGGAGGAGGAGGAGGTGGLLYGNGGAGGNGGSAAAA 204 G G G N GA GN GG G G GGA G G G+G + G + Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 205 GGAGGNALLFGNGGNGGSGASGGAAGHAGTIFGNGGNAGAGSG 247 G GGN GN G G +A A FG + G+G Sbjct: 63 GNGGGN----GNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAG 101 Score = 39.7 bits (92), Expect = 6e-05 Identities = 38/115 (33%), Positives = 46/115 (40%), Gaps = 10/115 (8%) Query: 497 SGVGGAGGSGGTALLLGS--GGAGGNGGTGGANSGSLFASPGGTGGAGGHGGAGGLIWGN 554 SG G G + G G+ GG G G GGA+ GS ++S G G G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 555 GGAGGNGGNGGTTADGALEGGTGGIGGTGGSAIAFGNGGQG--GAGGTGGDHSGG 607 G GG GN G + GTGG + +AFG GAGG S G Sbjct: 62 HGNGGGNGNSGGGS------GTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAG 110 Score = 39.3 bits (91), Expect = 7e-05 Identities = 25/85 (29%), Positives = 33/85 (38%) Query: 554 NGGAGGNGGNGGTTADGALEGGTGGIGGTGGSAIAFGNGGQGGAGGTGGDHSGGNGIGGK 613 +GG G G + G + GG G+G GG++ G + G G G G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 614 GGASGNGGNAGQVFGDGGTGGTGGA 638 G G GN+G G GG A Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAA 86 Score = 37.8 bits (87), Expect = 2e-04 Identities = 34/95 (35%), Positives = 39/95 (41%), Gaps = 10/95 (10%) Query: 591 NGGQGGAGGTGGDHSGGNGIGGKGGASGNGGNAGQVFGDG-GTGGTGGAGGAGSGTKAGG 649 +GG G TG + GN GG G GG + G G + GG+GSG GG Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASD---GSGWSSENNPWGGGSGSGIHWGG 58 Query: 650 TGSDGGHGGNATLIGNGGDGGAGGAGGAGSPAGAP 684 G G GNG GG G GG S AP Sbjct: 59 GSGHGNGG------GNGNSGGGSGTGGNLSAVAAP 87 Score = 37.4 bits (86), Expect = 3e-04 Identities = 32/105 (30%), Positives = 41/105 (39%), Gaps = 3/105 (2%) Query: 258 NGGDGGSSTSKAGGAGGNALFGNGGDGGSSTVAAGGAGGNTLVGNGGAGGAGGTSGLTGS 317 +GGDG + A GN G G G + G + GG G+G G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 318 GVAGGAGGSVGLWGSGGAGGDGGAATSLLGVGMNA-GAGGAGGNA 361 GG G+ G G G GG+ A + + G A GAGG A Sbjct: 62 HGNGGGNGNSG--GGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 36.2 bits (83), Expect = 7e-04 Identities = 35/122 (28%), Positives = 49/122 (40%), Gaps = 14/122 (11%) Query: 171 GAGGAGGAGGAGGTGGLLYGNGGAGGNGGSAAAAGGAGGNALLFGNGGNGGSGASGGAAG 230 G G G GA T G + NGG G G A+ G+G ++ GG GSG Sbjct: 3 GGDGRGHNTGAHSTSGNI--NGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGI------ 54 Query: 231 HAGTIFGNGGNAGAGSGLAGADGGLFGNGGDGGSSTSKAGGAGGNALFGNGGDGGSSTVA 290 GG +G G+G + G G S+ + G AL G G + +++ Sbjct: 55 ------HWGGGSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSIS 108 Query: 291 AG 292 AG Sbjct: 109 AG 110 Score = 35.8 bits (82), Expect = 8e-04 Identities = 34/108 (31%), Positives = 38/108 (35%), Gaps = 4/108 (3%) Query: 537 GTGGAGGHGGAGGLIWGNGGAGGNGGNGGTTADGALEGGTGGIGGTGGSAIAFGNGGQGG 596 G G G G I NGG G G GG + GG GS I GG G Sbjct: 6 GRGHNTGAHSTSGNI--NGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGI--HWGGGSG 61 Query: 597 AGGTGGDHSGGNGIGGKGGASGNGGNAGQVFGDGGTGGTGGAGGAGSG 644 G GG+ + G G G G S F T G GG + S Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISA 109 Score = 35.5 bits (81), Expect = 0.001 Identities = 25/112 (22%), Positives = 33/112 (29%), Gaps = 2/112 (1%) Query: 224 ASGGAAGHAGTIFGNGGNAGAGSGLAGADGGLFGNGGDGGSSTSKAGGAGGNALFGNGGD 283 + G GH GN G G GG + G G SS + G G + GG Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGG--ASDGSGWSSENNPWGGGSGSGIHWGGG 59 Query: 284 GGSSTVAAGGAGGNTLVGNGGAGGAGGTSGLTGSGVAGGAGGSVGLWGSGGA 335 G G G G ++ G + + S GA Sbjct: 60 SGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGA 111 Score = 34.7 bits (79), Expect = 0.002 Identities = 34/115 (29%), Positives = 44/115 (38%), Gaps = 3/115 (2%) Query: 165 GAGGAGGAGGAGGAGGA--GGTGGLLYGNGGAGGNGGSAAAAGGAGGNALLFGNGGNGGS 222 G G G GA G GG GL G G + G+G S+ GG+ GG G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 223 GASGGAAGHAGTIFGNGGNAGAGSGLAGADGGLFGNGGDGGSSTSKAGGAGGNAL 277 G GG G G + + +A L G GG + S + GA A+ Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPAL-STPGAGGLAVSISAGALSAAI 116 Score = 34.3 bits (78), Expect = 0.002 Identities = 33/109 (30%), Positives = 45/109 (41%), Gaps = 8/109 (7%) Query: 331 GSGGAGGDGGAATSLLGVGMNAGAGGAGGNAGLLYGNGGAGGAGGNGGDTTVPLFDSGVG 390 G G G + GA ++ +N G G G G G+G + GG + + G Sbjct: 3 GGDGRGHNTGAHST--SGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGS 60 Query: 391 GAGGAGGNASLFGNGGTGGVGGKGGTSSDLASATSGAGGAGGAGGVGGL 439 G G G GNG +GG G GG S +A+ + A G GGL Sbjct: 61 GHGNGG------GNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGL 103 Score = 34.3 bits (78), Expect = 0.003 Identities = 31/106 (29%), Positives = 39/106 (36%), Gaps = 3/106 (2%) Query: 294 AGGNTLVGNGGAGGAGGTSGLTGSGVAGGAGGSVGLWGSGGAGGDGGAATSLLGVGMNAG 353 +GG+ N GA G +G+ G G S G S GG + S + G +G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 354 AGGAGGNAGLLYGNGGAGGAGGNGGDTTVPLFDSGVGGAGGAGGNA 399 G GGN GG+G G F GAGG A Sbjct: 62 HGNGGGNGN---SGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 33.1 bits (75), Expect = 0.007 Identities = 29/81 (35%), Positives = 36/81 (44%), Gaps = 4/81 (4%) Query: 575 GTGGIGGTGGSAIAFGN--GGQGGAGGTGGDHSGGNGIGGKGGASGNGGNAGQVFGDGGT 632 G G G G+ GN GG G G GG S G+G + G G +G +G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGA-SDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 633 GGTGGAGG-AGSGTKAGGTGS 652 G GG G +G G+ GG S Sbjct: 62 HGNGGGNGNSGGGSGTGGNLS 82 Score = 32.8 bits (74), Expect = 0.007 Identities = 37/125 (29%), Positives = 45/125 (36%), Gaps = 11/125 (8%) Query: 390 GGAGGAGGNASLFGNGGTGGVGGKGGTSSDLA-SATSGAGGAGGAGGVGGLLYGNGGNGG 448 GA GN NGG G+G GG S S+ + G G G+ GNGG Sbjct: 11 TGAHSTSGNI----NGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGG 66 Query: 449 AGGIGGAAINILANAGAGGAGGAAGSSFIGNGGNGGAGGAGGAAALFSSGVGGAGGSGGT 508 G G +G GG A + GAGG A S+G A + Sbjct: 67 GNGNSG------GGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAAIADIM 120 Query: 509 ALLLG 513 A L G Sbjct: 121 AALKG 125 Score = 32.4 bits (73), Expect = 0.010 Identities = 28/103 (27%), Positives = 33/103 (32%) Query: 367 NGGAGGAGGNGGDTTVPLFDSGVGGAGGAGGNASLFGNGGTGGVGGKGGTSSDLASATSG 426 +GG G G +T + G G G GG + G G G S SG Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 427 AGGAGGAGGVGGLLYGNGGNGGAGGIGGAAINILANAGAGGAG 469 G GG G GG G L+ GAGG Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 30.8 bits (69), Expect = 0.034 Identities = 31/86 (36%), Positives = 39/86 (45%), Gaps = 10/86 (11%) Query: 464 GAGGAGGAAGSSFIGNGGNGGAGGAGGAA--ALFSSGVGGAGGSGGTALLLGSGGAGGNG 521 G G GA +S NGG G G GGA+ + +SS GG G+ + G G GNG Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNG 65 Query: 522 GTGGANSGSLFASPGGTGGAGGHGGA 547 G G + GG G GG+ A Sbjct: 66 GGNG--------NSGGGSGTGGNLSA 83 Score = 30.5 bits (68), Expect = 0.037 Identities = 34/104 (32%), Positives = 39/104 (37%), Gaps = 9/104 (8%) Query: 442 GNGGNGGAGGIGGAAINILANAGAGGAGGAAGSSFIGNGGNGGAGGAGGAAALFSSGVGG 501 G G N GA G G G GGA+ S + N GG+G G Sbjct: 6 GRGHNTGAHSTSGNING--GPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHG 63 Query: 502 AGGSGGTALLLGSGGAGGNGGTGGANSGSL-FASPG-GTGGAGG 543 GG G SGG G GG A + + F P T GAGG Sbjct: 64 NGGGNG-----NSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG 102
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv1654	CARBMTKINASE	50	4e-09	Bacterial carbamate kinase signature.
>CARBMTKINASE#Bacterial carbamate kinase signature. Length = 314 Score = 49.8 bits (119), Expect = 4e-09 Identities = 32/126 (25%), Positives = 51/126 (40%), Gaps = 12/126 (9%) Query: 172 GRIPVVSTLAPDADGVVHNINADTAAAAVAEALGAEKLLMLTDIDGLYTRW--PDRDSLV 229 G +PV+ + GV I+ D A +AE + A+ ++LTD++G + L Sbjct: 195 GGVPVILE-DGEIKGVEAVIDKDLAGEKLAEEVNADIFMILTDVNGAALYYGTEKEQWL- 252 Query: 230 SEIDTGTLAQLLPTL---ESGMVPKVEACLRAVIGGVPSAHIIDGRVTHCVLVELFTDAG 286 E+ L + M PKV A +R + G A I H + Sbjct: 253 REVKVEELRKYYEEGHFKAGSMGPKVLAAIRFIEWGGERAII-----AHLEKAVEALEGK 307 Query: 287 TGTKVV 292 TGT+V+ Sbjct: 308 TGTQVL 313 Score = 36.0 bits (83), Expect = 1e-04 Identities = 18/62 (29%), Positives = 28/62 (45%), Gaps = 10/62 (16%) Query: 28 GKVVVVKYGGNAMTDDTLRRAFAADMAFLRNC----------GIHPVVVHGGGPQITAML 77 GK VV+ GGNA+ + ++ M +R G V+ HG GPQ+ ++L Sbjct: 2 GKRVVIALGGNALQQRGQKGSYEEMMDNVRKTARQIAEIIARGYEVVITHGNGPQVGSLL 61 Query: 78 RR 79 Sbjct: 62 LH 63
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv1657	ARGREPRESSOR	168	2e-56	Bacterial arginine repressor signature.
>ARGREPRESSOR#Bacterial arginine repressor signature. Length = 149 Score = 168 bits (427), Expect = 2e-56 Identities = 47/148 (31%), Positives = 80/148 (54%), Gaps = 6/148 (4%) Query: 17 NRAGRQARIVAILSSAQVRSQNELAALLAAEGIEVTQATLSRDLEELGAVKLRGADGGTG 76 N+ R +I I+++ ++ +Q+EL +L +G VTQAT+SRD++EL VK+ + G+ Sbjct: 2 NKGQRHIKIREIITANEIETQDELVDILKKDGYNVTQATVSRDIKELHLVKVPT-NNGSY 60 Query: 77 IYVVPEDGSPVRGVSGGTDRMARLLGELLVSTDDSGNLAVLRTPPGAAHYLASAIDRAAL 136 Y +P D ++ R L + V D + +L VL+T PG A + + +D Sbjct: 61 KYSLPADQR-----FNPLSKLKRSLMDAFVKIDSASHLIVLKTMPGNAQAIGALMDNLDW 115 Query: 137 PQVVGTIAGDDTILVVAREPTTGAQLAG 164 +++GTI GDDTIL++ R + Sbjct: 116 EEIMGTICGDDTILIICRTHDDTKVVQK 143
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv1663	NUCEPIMERASE	45	3e-07	Nucleotide sugar epimerase signature.
>NUCEPIMERASE#Nucleotide sugar epimerase signature. Length = 334 Score = 45.2 bits (107), Expect = 3e-07 Identities = 32/136 (23%), Positives = 52/136 (38%), Gaps = 15/136 (11%) Query: 65 TVLITGGTGMAGSAVARHLVSRYGVRQVVLASRAGEHTESVA-ALVDELGSAGARVQVVS 123 L+TG G G V++ L+ G + V + + + S+ A ++ L G Q Sbjct: 2 KYLVTGAAGFIGFHVSKRLLEA-GHQVVGIDNLNDYYDVSLKQARLELLAQPG--FQFHK 58 Query: 124 CDVADRDAVAGLVASQPDLTAVFH---AAGVLDDAVITGLTPERVDKVLRAKVDGAWNLH 180 D+ADR+ + L AS VF V + E + + G N+ Sbjct: 59 IDLADREGMTDLFASGH-FERVFISPHRLAVRY-------SLENPHAYADSNLTGFLNIL 110 Query: 181 ELTRHLDVSAFVLFSS 196 E RH + + SS Sbjct: 111 EGCRHNKIQHLLYASS 126
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv1667c	YERSINIAYOPE	30	0.005	Yersinia virulence determinant YopE protein signature.
>YERSINIAYOPE#Yersinia virulence determinant YopE protein signature. Length = 219 Score = 30.1 bits (67), Expect = 0.005 Identities = 18/83 (21%), Positives = 34/83 (40%), Gaps = 1/83 (1%) Query: 132 APAPAERPAPPAMSGAQRRATEKELAAVDRQLARL-ADRVAAKHTELAEHDQSDHVGITR 190 AP PA+ P+P + S + ++ + L +QL L A+ + H + A IT+ Sbjct: 90 APTPAQMPSPTSFSDSIKQLAAETLPKYMQQLNSLDAEMLQKNHDQFATGSGPLRGSITQ 149 Query: 191 LTQQLRVLQDHVAAMENRWLELS 213 ++ + A + L Sbjct: 150 CQGLMQFCGGELQAEASAILNTP 172

Rv1749c

Rv1773c

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
Rv1749c	2	13	-3.176656	Possible integral membrane protein
Rv1750c	2	15	-3.403990	Possible fatty-acid-CoA ligase FadD1
Rv1751	2	15	-3.304682	Probable oxidoreductase
Rv1752	2	15	-3.653477	Conserved hypothetical protein
Rv1753c	3	14	-3.675806	PPE family protein PPE24
Rv1754c	5	15	2.413012	Conserved protein
Rv1755c	5	14	3.162833	Probable phospholipase C 4 (fragment) PlcD
Rv1756c	4	15	4.073521	Putative transposase
Rv1757c	5	13	4.050300	Putative transposase for insertion sequence
Rv1758	5	13	4.050300	Probable cutinase Cut1
Rv1759c	2	15	3.617765	PE-PGRS family protein Wag22
Rv1760	-1	14	-1.527297	Possible triacylglycerol synthase
Rv1761c	0	17	-2.229688	Possible exported protein
Rv1762c	0	17	-2.241055	Unknown protein
Rv1763	0	18	-1.778053	Putative transposase for insertion sequence
Rv1764	1	14	1.918228	Putative transposase
Rv1765c	3	16	1.958583	Conserved hypothetical protein
Rv1765A	1	12	2.603334	Putative transposase (fragment)
Rv1766	0	12	1.958866	Conserved protein
Rv1767	0	13	1.903738	Conserved protein
Rv1768	1	11	1.912804	PE-PGRS family protein PE_PGRS31
Rv1769	2	13	-0.436459	Conserved protein
Rv1770	3	13	-0.197161	Conserved protein
Rv1771	3	13	-0.907999	L-gulono-1,4-lactone dehydrogenase
Rv1772	2	11	-0.803732	Hypothetical protein
Rv1773c	2	12	-0.704726	Probable transcriptional regulatory protein

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv1753c	CHANLCOLICIN	35	0.002	Channel forming colicin signature.
>CHANLCOLICIN#Channel forming colicin signature. Length = 522 Score = 34.7 bits (79), Expect = 0.002 Identities = 30/102 (29%), Positives = 42/102 (41%), Gaps = 10/102 (9%) Query: 50 SGLVGGAWQGASSSAMAAAAAPYAAWLAA--AAVQAEQT--AAQAAAMIAEFEAVKTAVV 105 SG GG +G S S +AA A W A QAEQ A AA A+ +A + A+ Sbjct: 32 SGSGGGGGKGGSKSESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALT 91 Query: 106 QPMLVAANRADLVSLVMSNLFGQNAPAIAAIEATYEQMWAAD 147 Q + D+V+ + + + A A M A D Sbjct: 92 QRL------KDIVNEALRHNASRTPSATELAHANNAAMQAED 127
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv1754c	PF05616	42	6e-06	Neisseria meningitidis TspB protein
>PF05616#Neisseria meningitidis TspB protein Length = 501 Score = 41.7 bits (97), Expect = 6e-06 Identities = 37/102 (36%), Positives = 41/102 (40%), Gaps = 20/102 (19%) Query: 154 LNPG---ARNAAPLQQQALVPRANPGPNPAPNP-PATGPQPPNATQLTPNPAPAPDPAPA 209 L PG A NA PL + + P NP NPAPN P T PNP P PD P Sbjct: 315 LTPGSAEAPNAQPLPE--VSPAENPANNPAPNENPGT----------RPNPEPDPDLNPD 362 Query: 210 AAPDPGATLAGATTSLAEWVTGPDSPNKTLERFGISGTDLGI 251 A PD S A PD PN + G D G+ Sbjct: 363 ANPDTDGQPGTRPDSPAV----PDRPNGRHRKERKEGEDGGL 400
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv1759c	cloacin	37	2e-04	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 37.4 bits (86), Expect = 2e-04 Identities = 36/110 (32%), Positives = 47/110 (42%), Gaps = 1/110 (0%) Query: 243 AGGAGGAGGAGGLFTTGGV-GGAGGQGHTGGAGGAGGAGGLFGAGGMGGAGGFGDHGTLG 301 +GG G G T+G + GG G G GGA G G G G G G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 302 TGGAGGDGGGGGLFGAGGDGGAGGSGLTTGGAAGNGGNAGTLSLGAAGGA 351 G GG+G GG G GG+ A + + G A + AG L++ + GA Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGA 111 Score = 37.0 bits (85), Expect = 3e-04 Identities = 39/112 (34%), Positives = 52/112 (46%), Gaps = 7/112 (6%) Query: 673 NGGAGGAGGTGSTAGGAGGAGGAGGLYAHGGTGGPGGNGGSTGAGGTGGAGGPGGLYGAG 732 +GG G TG+ + GG GL G G G+G S+ GG G G +G G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGL--GVGGGASDGSGWSSENNPWGGGSGSGIHWGGG 59 Query: 733 GSGGAGGHGGMAGGGGGVGGNAGSLTLNASGG-----AGGSGGSSLSGKAGA 779 G GG G +GGG G GGN ++ + G G+GG ++S AGA Sbjct: 60 SGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGA 111 Score = 37.0 bits (85), Expect = 4e-04 Identities = 30/101 (29%), Positives = 43/101 (42%) Query: 331 GGAAGNGGNAGTLSLGAAGGAGGTGGAGGTVFGGGKGGAGGAGGNAGMLFGSGGGGGTGG 390 GA GN G G G + G+G + GG G+G + G G G GGG G Sbjct: 11 TGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNGN 70 Query: 391 FGFAAGGQGGVGGSAGMLSGSGGSGGAGGSGGPAGTAAGGA 431 G +G G + A ++ + G+GG A + + GA Sbjct: 71 SGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGA 111 Score = 36.2 bits (83), Expect = 7e-04 Identities = 34/113 (30%), Positives = 42/113 (37%), Gaps = 6/113 (5%) Query: 263 GAGGQGHTGGAGGA-----GGAGGLFGAGGMGGAGGFGDHGTLGTGGAGGDGGGGGLFGA 317 G G+GH GA GG GL GG G+ GG+G GG G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 318 GGDGGAGGSGLTTGGAAGNGGNAGTLSLG-AAGGAGGTGGAGGTVFGGGKGGA 369 G GG G SG +G A ++ G A G GG ++ G A Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAA 115 Score = 35.8 bits (82), Expect = 7e-04 Identities = 33/103 (32%), Positives = 41/103 (39%) Query: 683 GSTAGGAGGAGGAGGLYAHGGTGGPGGNGGSTGAGGTGGAGGPGGLYGAGGSGGAGGHGG 742 G G GA G G TG G G S G+G + GG G+G G G G Sbjct: 4 GDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHG 63 Query: 743 MAGGGGGVGGNAGSLTLNASGGAGGSGGSSLSGKAGAGGAGGS 785 GG G GG +G+ ++ A + G GAGG S Sbjct: 64 NGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVS 106 Score = 35.5 bits (81), Expect = 0.001 Identities = 24/74 (32%), Positives = 32/74 (43%) Query: 534 NGDSGTPGTGDDGGAGGWLFGNGGNGGAGAAGTNGSAGGAGGAGGILFGTGGAGGAGGVG 593 N + + +GG G G G + G+G + N GG G+G G G G GG G Sbjct: 10 NTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNG 69 Query: 594 TAGAGGAGGAGGSA 607 +G G G SA Sbjct: 70 NSGGGSGTGGNLSA 83 Score = 35.1 bits (80), Expect = 0.001 Identities = 24/81 (29%), Positives = 30/81 (37%) Query: 644 LGGCGGGAFTAGVTTGGAGGTGGAAGLFANGGAGGAGGTGSTAGGAGGAGGAGGLYAHGG 703 + G G G + GG GL GGA G S GG G+G + G Sbjct: 1 MSGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGS 60 Query: 704 TGGPGGNGGSTGAGGTGGAGG 724 G GG G++G G G Sbjct: 61 GHGNGGGNGNSGGGSGTGGNL 81 Score = 35.1 bits (80), Expect = 0.001 Identities = 34/101 (33%), Positives = 42/101 (41%), Gaps = 5/101 (4%) Query: 117 NGANGAPGTGANGGDAGWLIGNGGAGGSGAKGAN---GGAGGPGGAAGLFGNGGAGGAGG 173 N + NGG G +G G + GSG N GG G G G G GG G Sbjct: 10 NTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNG 69 Query: 174 TATANNGIGGAGGAGGSAMLFG--AGGAGGAGGAATSLVGG 212 + +G GG A + + FG A GAGG A S+ G Sbjct: 70 NSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAG 110 Score = 35.1 bits (80), Expect = 0.001 Identities = 36/103 (34%), Positives = 40/103 (38%), Gaps = 14/103 (13%) Query: 368 GAGGAGGNAGMLFGSGGGGGTGGFGFAAGGQGGVGGSAGMLSGSGGSGGAGGSGGPAGTA 427 G G G N G SG GG G+G G GSG S GG +G+ Sbjct: 3 GGDGRGHNTGAHSTSGN---------INGGPTGLGVGGGASDGSGWSSENNPWGGGSGSG 53 Query: 428 AGGAGGAGGAPGLIGNGGNGGNGGESGGTGGVGGAGGNAVLIG 470 GG+G GNGG GN G GTGG A V G Sbjct: 54 IHWGGGSGH-----GNGGGNGNSGGGSGTGGNLSAVAAPVAFG 91 Score = 34.7 bits (79), Expect = 0.002 Identities = 35/117 (29%), Positives = 44/117 (37%), Gaps = 2/117 (1%) Query: 137 GNGGAGGSGAKGANGGAGGPGGAAGLFGNGGAGGAGGTATANNGIGGAGGAGGSAMLFGA 196 G+G +GA +G G G GL GGA G ++ NN GG G+G Sbjct: 4 GDGRGHNTGAHSTSGNING--GPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 197 GGAGGAGGAATSLVGGIGGTGGTGGNAGMLAGAAGAGGAGGFSFSTAGGAGGAGGAG 253 G GG G + G G A GAGG + S + GA A A Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAAIAD 118 Score = 33.5 bits (76), Expect = 0.004 Identities = 40/129 (31%), Positives = 49/129 (37%), Gaps = 12/129 (9%) Query: 733 GSGGAGGHGGMAGGGGGVGGNAGSLTLNASGGAGGSGGSSLSGKAGAGGAGGSAGLFYGS 792 G G G + G G + G L G G S GS S + G G +G+ +G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGL----GVGGGASDGSGWSSENNPWGGGSGSGIHWGG 58 Query: 793 GGAGGNGGYSLNGTGGDGGTGGAGQITGLRSGFG-------GAGGAGGASDTGAGGNGGA 845 G GNGG + +GG GTGG FG GAGG + GA A Sbjct: 59 GSGHGNGGGN-GNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAAIA 117 Query: 846 GGKAGLYGN 854 A L G Sbjct: 118 DIMAALKGP 126 Score = 33.1 bits (75), Expect = 0.005 Identities = 32/125 (25%), Positives = 47/125 (37%), Gaps = 2/125 (1%) Query: 703 GTGGPGGNGGSTGAGGTGGAGGPGGLYGAGGSGGAGGHGGMAGGGGGVGGNAGSLTLNAS 762 G G G N G+ G G G G G S G+G GGG G + Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 763 GGAGGSGGSSLSGKAGAGGAGGSAGLFYG--SGGAGGNGGYSLNGTGGDGGTGGAGQITG 820 G GG+G S G + +A + +G + G GG +++ + G A + Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAAIADIMAA 122 Query: 821 LRSGF 825 L+ F Sbjct: 123 LKGPF 127 Score = 32.8 bits (74), Expect = 0.007 Identities = 31/108 (28%), Positives = 36/108 (33%) Query: 289 GGAGGFGDHGTLGTGGAGGDGGGGGLFGAGGDGGAGGSGLTTGGAAGNGGNAGTLSLGAA 348 GG G + G T G G G G G G+G S G+G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 349 GGAGGTGGAGGTVFGGGKGGAGGAGGNAGMLFGSGGGGGTGGFGFAAG 396 G GG G +GG GG A A G S G G +AG Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAG 110 Score = 31.6 bits (71), Expect = 0.017 Identities = 33/123 (26%), Positives = 43/123 (34%), Gaps = 8/123 (6%) Query: 226 LAGAAGAGGAGGFSFSTAGGAGGAGGAGGLFTTGGVGGAGGQGHTGGAGGAGGAGGLFGA 285 ++G G G G + ST+G G G+ G + GG+G G Sbjct: 1 MSGGDGRGHNTG-AHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGG 59 Query: 286 GGMGGAGGFGDHGTLGTGGAGGDGGGGGLFGAGGDGGAGGSGLTTGGAAGNGGNAGTLSL 345 G G GG GG G GG L G L+T GA G + +L Sbjct: 60 SGHGNGGG-------NGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGAL 112 Query: 346 GAA 348 AA Sbjct: 113 SAA 115 Score = 30.8 bits (69), Expect = 0.026 Identities = 29/109 (26%), Positives = 39/109 (35%), Gaps = 2/109 (1%) Query: 803 LNGTGGDGGTGGAGQITGLRSGFGGAGGAGGASDTGAGGNGGAGGKAGLYGNGGDGGAGG 862 ++G G G GA +G +G G GG + G+G + G G+G G G Sbjct: 1 MSGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGS 60 Query: 863 DGATSGKGGAGGNAVVIGNGGNGGNAGKAGG--TAGAGGAGGLVLGRDG 909 G G G G + A A G GAGGL + Sbjct: 61 GHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISA 109 Score = 30.5 bits (68), Expect = 0.036 Identities = 23/81 (28%), Positives = 32/81 (39%) Query: 555 NGGNGGAGAAGTNGSAGGAGGAGGILFGTGGAGGAGGVGTAGAGGAGGAGGSAFLIGSGG 614 +GG+G G + ++G G L GGA G + GG+G G G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 615 TGGVGGAATTTGGVGGAGGNA 635 G GG + GG G G + Sbjct: 62 HGNGGGNGNSGGGSGTGGNLS 82 Score = 30.5 bits (68), Expect = 0.037 Identities = 26/86 (30%), Positives = 33/86 (38%), Gaps = 8/86 (9%) Query: 410 GSGGSGGAGGSGGPAGTAAGGAGGAGGAPGLIG--------NGGNGGNGGESGGTGGVGG 461 G G + GA + G G G GGA G GG+G GG+G G Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNG 65 Query: 462 AGGNAVLIGNGGEGGIGALAGKSGFG 487 G G+G G + A+A FG Sbjct: 66 GGNGNSGGGSGTGGNLSAVAAPVAFG 91
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv1768	cloacin	36	3e-04	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 36.2 bits (83), Expect = 3e-04 Identities = 48/164 (29%), Positives = 59/164 (35%), Gaps = 28/164 (17%) Query: 141 GNGGNGGSGGVNQAGGNGGNAGLWGNGGSGGAGGNATTAGRNGFNGGAGGSGGLLWGNGG 200 G G G + G + GN N G G G GGA + + N GG GSG G G Sbjct: 3 GGDGRGHNTGAHSTSGNI-NGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 201 AGGAGGNGGPAPLVGGVGTTGGAGGNGGGAGLFYGFGGAGGNGGMGGVAPSTGPSMGILP 260 G GGNG +G GG+G G + VA L Sbjct: 62 HGNGGGNGN----------------SG---------GGSGTGGNLSAVAAPVAFGFPALS 96 Query: 261 AGGVGGPGGSGGASALAFGSGGVGGAGGLGGPTDGTVQGVGGFG 304 G GG S A AL+ + A L GP + GV +G Sbjct: 97 TPGAGGLAVSISAGALSAAIADIMAA--LKGPFKFGLWGVALYG 138 Score = 35.5 bits (81), Expect = 7e-04 Identities = 32/106 (30%), Positives = 39/106 (36%), Gaps = 2/106 (1%) Query: 297 VQGVGGFGGQGGNGGQSGLLFGNAGAGGAGAAGGAGTGDTESFGGHGGAGGDGGAVGLIG 356 + G G G G SG + N G G G GGA G S + GG G + G Sbjct: 1 MSGGDGRGHNTGAHSTSGNI--NGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGG 58 Query: 357 NGGAGGTGSPGAVVGGNGGVGGLGGAGSPGGLLYGTGGAGGNGGPG 402 G G G G GG+G G L +P + G GG Sbjct: 59 GSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 35.5 bits (81), Expect = 7e-04 Identities = 31/87 (35%), Positives = 35/87 (40%), Gaps = 5/87 (5%) Query: 471 GVGGMGGNGGATSVGGTLYAAGGNGGDGGLVWGNGGTGGSGGAGGAGSVGNGGAGGNAAL 530 G G G N GA S G + NGG GL G G + GSG + G G G Sbjct: 3 GGDGRGHNTGAHSTSGNI-----NGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWG 57 Query: 531 LFGNGGAGGAGGAGGIGAGGAGGFGAV 557 G GG G G G+G G AV Sbjct: 58 GGSGHGNGGGNGNSGGGSGTGGNLSAV 84 Score = 33.1 bits (75), Expect = 0.003 Identities = 28/87 (32%), Positives = 37/87 (42%), Gaps = 7/87 (8%) Query: 471 GVGGMGGNGGATSVGG--TLYAAGGNGGDGGLVWGNGGTGGSGGAGGAGSVGNGGAGGNA 528 G G+G GGA+ G + G G G+ WG G G+GG G G+G G + Sbjct: 23 GPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNGNSGGGSGTGGNLS 82 Query: 529 ALLFGNGGAGGAGGAGGIGAGGAGGFG 555 A+ A A G + GAGG Sbjct: 83 AV-----AAPVAFGFPALSTPGAGGLA 104 Score = 32.8 bits (74), Expect = 0.005 Identities = 31/103 (30%), Positives = 35/103 (33%), Gaps = 5/103 (4%) Query: 266 GPGGSGGASALAFGSGGVGGAGGLGGPTDGTVQGVGGFGGQGGNGGQSGLLFGNAGAGGA 325 G G G + SG + G G G G G GG SG G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 326 GAAGGAGTGDTESFGGHGGAGGDGGAVGLIGNGGAGGTGSPGA 368 G GG G GG G GG+ AV G +PGA Sbjct: 63 GNGGGNGNS-----GGGSGTGGNLSAVAAPVAFGFPALSTPGA 100 Score = 32.4 bits (73), Expect = 0.006 Identities = 34/108 (31%), Positives = 37/108 (34%), Gaps = 10/108 (9%) Query: 510 SGGAGGAGSVGNGGAGGNAALLFGNGGAGGAGGAGGIGAGGAGGFGAVLFGNGGAGGSGA 569 SGG G + G GN NGG G G GG G +G G G Sbjct: 2 SGGDGRGHNTGAHSTSGNI-----NGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHW 56 Query: 570 PGGIGAGGNGGNALLVGNGGNGGAGTGGAAGGAGGSGGLLFGQNGMPG 617 GG G G GGN GG+GTGG F PG Sbjct: 57 GGGSGHGNGGGN-----GNSGGGSGTGGNLSAVAAPVAFGFPALSTPG 99 Score = 32.4 bits (73), Expect = 0.007 Identities = 28/99 (28%), Positives = 33/99 (33%) Query: 279 GSGGVGGAGGLGGPTDGTVQGVGGFGGQGGNGGQSGLLFGNAGAGGAGAAGGAGTGDTES 338 G G GA G +G G+G GG G S G G+G G G+G Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNG 65 Query: 339 FGGHGGAGGDGGAVGLIGNGGAGGTGSPGAVVGGNGGVG 377 G GG G L G P G GG+ Sbjct: 66 GGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 31.6 bits (71), Expect = 0.010 Identities = 25/99 (25%), Positives = 30/99 (30%), Gaps = 7/99 (7%) Query: 129 TGQNGGDGGILYGNGGNGGSGGVNQAGGNGGNAGLWGNGGSGGAGGNATTAGRNGFNGGA 188 TG + G I G G G GG + G WG G G + NG G Sbjct: 11 TGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNGN 70 Query: 189 GGSGGLLWGNGGAGGAGGNGGPAPLVGGVGTTGGAGGNG 227 G G G + AP+ G G G Sbjct: 71 SG-------GGSGTGGNLSAVAAPVAFGFPALSTPGAGG 102 Score = 30.8 bits (69), Expect = 0.017 Identities = 31/93 (33%), Positives = 41/93 (44%), Gaps = 3/93 (3%) Query: 132 NGGDGGILYGNGGNGGSGGVNQAGGNGGNAGLWGNGGSGGAGGNATTAGRNGFNGGAGGS 191 NGG G+ G G + GSG ++ GG +G + G G GN G NG +GG G+ Sbjct: 21 NGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNG---GGNGNSGGGSGT 77 Query: 192 GGLLWGNGGAGGAGGNGGPAPLVGGVGTTGGAG 224 GG L G P GG+ + AG Sbjct: 78 GGNLSAVAAPVAFGFPALSTPGAGGLAVSISAG 110 Score = 30.8 bits (69), Expect = 0.019 Identities = 36/118 (30%), Positives = 46/118 (38%), Gaps = 6/118 (5%) Query: 332 GTGDTESFGGHGGAGG-DGGAVGLIGNGGAGGTGSPGAVVGGNGGVGGLGGAGSPGGLLY 390 G G + G H +G +GG GL GGA + GG G G G Sbjct: 4 GDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHG 63 Query: 391 GTGGAGGNGGPGGDGGTGATVGFAGSGGF-----GGAGGIAQLFGTGGMGGSGGGIGA 443 GG G +GG G GG + V + GF GAGG+A G + + I A Sbjct: 64 NGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAAIADIMA 121

Rv1798

Rv1814

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
Rv1798	2	12	0.151591	ESX conserved component EccA5. ESX-5 type VII
Rv1799	4	14	0.514962	Probable lipoprotein LppT
Rv1800	5	16	0.229880	PPE family protein PPE28
Rv1801	7	17	0.859467	PPE family protein PPE29
Rv1802	5	15	1.316270	PPE family protein PPE30
Rv1803c	6	17	1.633372	PE-PGRS family protein PE_PGRS32
Rv1804c	4	17	-0.040951	Conserved protein
Rv1805c	4	16	0.129291	Hypothetical protein
Rv1806	4	14	0.384833	PE family protein PE20
Rv1807	3	12	0.736747	PPE family protein PPE31
Rv1808	3	13	-0.036627	PPE family protein PPE32
Rv1809	2	12	-1.327541	PPE family protein PPE33
Rv1810	1	13	-1.480829	Conserved protein
Rv1811	-1	11	-1.397794	Possible Mg2+ transport P-type ATPase C MgtC
Rv1812c	-1	8	-1.501090	Probable dehydrogenase
Rv1813c	4	10	1.634178	Conserved hypothetical protein
Rv1814	2	9	0.489998	Membrane-bound C-5 sterol desaturase Erg3

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv1803c	cloacin	42	8e-06	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 41.6 bits (97), Expect = 8e-06 Identities = 31/76 (40%), Positives = 36/76 (47%), Gaps = 2/76 (2%) Query: 523 GAGGSGGLLFGSGGAGGIGGAGGVGGSGNDGGNGG--DGGQGGASGLGIGNGGPGGSGGT 580 G G + G SG G GVGG +DG + GG SG GI GG G G Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNG 65 Query: 581 GGAGGTGGSAGTGGAG 596 GG G +GG +GTGG Sbjct: 66 GGNGNSGGGSGTGGNL 81 Score = 36.6 bits (84), Expect = 3e-04 Identities = 26/85 (30%), Positives = 36/85 (42%), Gaps = 2/85 (2%) Query: 273 AGSGGLGGNGGNGAQGGWLIGNGGQGGDSGAGGGTDSTQTGVMNGASGGSAGIAGNGGDA 332 +G G G N G + G + NGG G GG +D + N GG +G + G Sbjct: 2 SGGDGRGHNTGAHSTSGNI--NGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGG 59 Query: 333 GLVGNGGAGGNGGNGAAGSALGTTI 357 GNGG GN G G+ + + Sbjct: 60 SGHGNGGGNGNSGGGSGTGGNLSAV 84 Score = 35.8 bits (82), Expect = 5e-04 Identities = 31/92 (33%), Positives = 36/92 (39%), Gaps = 8/92 (8%) Query: 133 GTGQNGGDGGWLYGNGGNGGSGGTGQNGGNGGSAGLWGSGGNGGQGGAGANGAAGQPGKA 192 G G+ G NGG G G GG +G W S N GG+G+ G Sbjct: 4 GDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSG-WSSENNPWGGGSGSGIHWGGGSGH 62 Query: 193 GGSGGNGGAGGWIYGHGGHGGAGGNGGNATAP 224 G GGNG + GG G GGN AP Sbjct: 63 GNGGGNGNS-------GGGSGTGGNLSAVAAP 87 Score = 35.8 bits (82), Expect = 6e-04 Identities = 25/79 (31%), Positives = 32/79 (40%) Query: 152 GSGGTGQNGGNGGSAGLWGSGGNGGQGGAGANGAAGQPGKAGGSGGNGGAGGWIYGHGGH 211 G G G N G ++G G G G GA+ +G + GG G+G G GH Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 212 GGAGGNGGNATAPGGASAG 230 G GGNG + G Sbjct: 63 GNGGGNGNSGGGSGTGGNL 81 Score = 33.9 bits (77), Expect = 0.002 Identities = 26/71 (36%), Positives = 28/71 (39%), Gaps = 1/71 (1%) Query: 339 GAGGNGGNGAAGSALGTTIFGGSGGVGGSGGDGGNGGWLFGSGASGGNGGQGGDAGTNGF 398 G G G N A S G I GG G+G GG GW + GG G G G Sbjct: 3 GGDGRGHNTGAHSTSGN-INGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 399 AGFGGSAGGGG 409 G GG G G Sbjct: 62 HGNGGGNGNSG 72 Score = 33.9 bits (77), Expect = 0.002 Identities = 23/85 (27%), Positives = 36/85 (42%) Query: 268 TAGDSAGSGGLGGNGGNGAQGGWLIGNGGQGGDSGAGGGTDSTQTGVMNGASGGSAGIAG 327 + GD G + GG G G G+G +++ G +G+ G +G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 328 NGGDAGLVGNGGAGGNGGNGAAGSA 352 +G G +GG G GGN +A +A Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAA 86 Score = 33.1 bits (75), Expect = 0.004 Identities = 27/80 (33%), Positives = 33/80 (41%), Gaps = 4/80 (5%) Query: 210 GHGGAGGNGGNATAPGGASAGFDGGAGGNGGSGGRGGLLFGNGGNGSVGGMGGQGTNDTA 269 G G G N G + G + G G G G S G G + N GG G G + Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSG----WSSENNPWGGGSGSGIHWGG 58 Query: 270 GDSAGSGGLGGNGGNGAQGG 289 G G+GG GN G G+ G Sbjct: 59 GSGHGNGGGNGNSGGGSGTG 78 Score = 31.6 bits (71), Expect = 0.011 Identities = 27/94 (28%), Positives = 29/94 (30%), Gaps = 17/94 (18%) Query: 170 GSGGNGGQGGAGANGAAGQPGKAGGSGGNGGAGGWIYGHGGHGGAGGNGGNATAPGGASA 229 G G N G N G P G GG GW + P G + Sbjct: 6 GRGHNTGAHSTSGN-INGGPTGLGVGGGASDGSGW--------------SSENNPWGGGS 50 Query: 230 GFDGGAGGNGGSGGRGGLLFGNGGNGSVGGMGGQ 263 G GG G G GG GN G GS G Sbjct: 51 GSGIHWGGGSGHGNGGG--NGNSGGGSGTGGNLS 82 Score = 31.6 bits (71), Expect = 0.011 Identities = 29/84 (34%), Positives = 34/84 (40%), Gaps = 5/84 (5%) Query: 553 GGNGGDGGQGGASGLGIGNGGPGGSGGTGGAGGTGGSAGTGGAGGDGGNAALLIGTGGDG 612 GG+G G S G NGGP G G GGA G + G G + + G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 613 GDGVPPAPGGQGGKGGLIGLPGQN 636 G+G GG G GG G G Sbjct: 63 GNG-----GGNGNSGGGSGTGGNL 81 Score = 31.2 bits (70), Expect = 0.015 Identities = 36/91 (39%), Positives = 40/91 (43%), Gaps = 6/91 (6%) Query: 500 NGATGGTGVGNIIQEAGGDGSDGGAGGSGGLLFGSG-GAGGIGGAGGVGGSGNDGGNGGD 558 NG G GVG + G S+ G G GSG GG G G GG+GN GG G Sbjct: 21 NGGPTGLGVGGGASDGSGWSSENNPWGGGS---GSGIHWGGGSGHGNGGGNGNSGGGSGT 77 Query: 559 GGQGGASGLGIGNGGPGGSGGTGGAGGTGGS 589 GG A + G P S T GAGG S Sbjct: 78 GGNLSAVAAPVAFGFPALS--TPGAGGLAVS 106 Score = 30.8 bits (69), Expect = 0.016 Identities = 28/80 (35%), Positives = 31/80 (38%), Gaps = 1/80 (1%) Query: 493 GNGGAGGNGATGGTGVGNIIQEAGGDGSDGGAGGSGGLLFGSGGAGGIGGAGGVGGSGND 552 G G G N T GNI G G GGA G + GG G+G G G+ Sbjct: 3 GGDGRGHNTGAHSTS-GNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 553 GGNGGDGGQGGASGLGIGNG 572 GNGG G G GN Sbjct: 62 HGNGGGNGNSGGGSGTGGNL 81 Score = 30.1 bits (67), Expect = 0.031 Identities = 28/70 (40%), Positives = 34/70 (48%), Gaps = 4/70 (5%) Query: 264 GTNDTAGD-SAGSGGLGGNGGNGAQGGWLIGNGGQGGDSGAG---GGTDSTQTGVMNGAS 319 G + T+G+ + G GLG GG GW N GG SG+G GG G NG S Sbjct: 12 GAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNGNS 71 Query: 320 GGSAGIAGNG 329 GG +G GN Sbjct: 72 GGGSGTGGNL 81

Rv1912c

Rv1918c

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
Rv1912c	2	17	-3.683964	Possible oxidoreductase FadB5
Rv1913	3	18	-4.440546	Conserved hypothetical protein
Rv1914c	4	18	-4.535668	Unknown protein
Rv1915	4	16	-4.388123	Probable isocitrate lyase AceAa [first part]
Rv1916	3	16	-4.615507	Probable isocitrate lyase AceAb [second part]
Rv1917c	4	16	-4.663982	PPE family protein PPE34
Rv1918c	1	13	-3.543616	PPE family protein PPE35

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv1917c	cloacin	36	0.001	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 35.8 bits (82), Expect = 0.001 Identities = 24/77 (31%), Positives = 33/77 (42%), Gaps = 3/77 (3%) Query: 240 NVGSNNVGLGNTGNGNIGFGNTGNGNIGFGLTGDNQQGFGGWNSGTGNIGLFNSGTGNIG 299 N G+++ GN G G G G + G G + +N GG SG G SG GN G Sbjct: 10 NTGAHSTS-GNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGG--GSGHGNGG 66 Query: 300 IGNTGTGNFGIGNSGTS 316 G G G + ++ Sbjct: 67 GNGNSGGGSGTGGNLSA 83 Score = 33.1 bits (75), Expect = 0.008 Identities = 26/76 (34%), Positives = 32/76 (42%), Gaps = 1/76 (1%) Query: 882 GFGNIGGNNYGFANIGNGNIGFGNTGTGNIGIGLTGDNQVGFGALNSGSGNIGFFNSGNG 941 G G+ G + NI G G G G + G G + +N G SG G GNG Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNG 65 Query: 942 NIGFFNSGNGNVGIGN 957 G NSG G+ GN Sbjct: 66 G-GNGNSGGGSGTGGN 80 Score = 31.2 bits (70), Expect = 0.038 Identities = 23/81 (28%), Positives = 28/81 (34%), Gaps = 1/81 (1%) Query: 247 GLGNTGNGNIGFGNTGNGNIGFGLTGDNQQGFGGWNSGTGNIGLFNSGTGNIGIGNTGTG 306 G G+ + GN G G G+ G G G + G SG G G G Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNG 65 Query: 307 NFGIGNSGTSYNTGIGNTGQA 327 GNSG TG + A Sbjct: 66 GGN-GNSGGGSGTGGNLSAVA 85 Score = 30.8 bits (69), Expect = 0.045 Identities = 36/116 (31%), Positives = 47/116 (40%), Gaps = 12/116 (10%) Query: 826 GSGNVGSYNFGSGNIGNGSFGFGNIGSNNFGFGNVGSNNLGFANTGPGLTEALHNIGFGN 885 G G+ + SGNI G G G G + G G NN +G G+ G Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHW------GGG 59 Query: 886 IGGNNYGFANIGNGNIGFGNTGTGNIGIGLTGDNQVGFGALNS-GSGNIGFFNSGN 940 G N G GNGN G G +GTG + GF AL++ G+G + S Sbjct: 60 SGHGNGG----GNGNSG-GGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAG 110
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv1918c	cloacin	36	0.001	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 35.8 bits (82), Expect = 0.001 Identities = 61/264 (23%), Positives = 89/264 (33%), Gaps = 41/264 (15%) Query: 378 NTGSFNVGHYNFGAFNPGPSNTGTFNTGGANTGWFNTGSINTGAFNIGDM---NNGLFNT 434 NTG+ + G N GP+ G +GW + + G G +G N Sbjct: 10 NTGAHSTS----GNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNG 65 Query: 435 GDMNNGVFYRGVGQGSLQFAITSPDLTLPSLEIPGISVPAFSLPAITLPSL---TIPAVT 491 G N G G G+L P+L PG A S+ A L + + A+ Sbjct: 66 GGNGNSGGGSGTG-GNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAAIADIMAALK 124 Query: 492 TPANVTVGAFDLPGLTVPSLTI---PAAMTPANITVGAFDLPGLTVPSLTIPATTTPANI 548 P + L G+ +PS P M+ ++ A D+ V SL + T N+ Sbjct: 125 GPFKFGLWGVALYGV-LPSQIAKDDPNMMSKIVTSLPADDITESPVSSLPLDKATVNVNV 183 Query: 549 TV-----------GAFNLPQLSIPSVTVPPITIPAGTALGAFNLPTLSI------PSVTV 591 V + +S+P V P P P L+I P+V Sbjct: 184 RVVDDVKDERQNISVVSGVPMSVPVVDAKPTERPGVFTASIPGAPVLNISVNNSTPAVQT 243 Query: 592 PPITI---------PAGTTVGGFT 606 + PAG T GG T Sbjct: 244 LSPGVTNNTDKDVRPAGFTQGGNT 267

Rv1929c

Rv1959c

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
Rv1929c	2	13	2.090277	Conserved hypothetical protein
Rv1930c	1	14	2.619188	Conserved hypothetical protein
Rv1931c	1	15	1.898868	Probable transcriptional regulatory protein
Rv1932	0	15	1.188046	Probable thiol peroxidase Tpx
Rv1933c	0	16	1.208119	Probable acyl-CoA dehydrogenase FadE18
Rv1934c	1	17	0.958610	Probable acyl-CoA dehydrogenase FadE17
Rv1935c	0	14	0.769523	Possible enoyl-CoA hydratase EchA13 (enoyl
Rv1936	1	14	0.797891	Possible monooxygenase
Rv1937	2	13	0.913063	Possible oxygenase
Rv1938	4	11	1.084445	Probable epoxide hydrolase EphB (epoxide
Rv1939	6	14	1.043645	Probable oxidoreductase
Rv1940	2	13	0.369256	Probable riboflavin biosynthesis protein RibA1
Rv1941	1	15	-0.799407	Probable short-chain type
Rv1942c	0	16	-1.077593	Possible toxin MazF5
Rv1943c	-1	18	-2.479065	Possible antitoxin MazE5
Rv1944c	-1	20	-2.554107	Conserved protein
Rv1945	-1	20	-3.098326	Conserved hypothetical protein
Rv1946c	-2	24	-3.561927	Possible lipoprotein
Rv1947	0	23	-3.324770	Hypothetical protein
Rv1948c	1	27	-4.213295	Hypothetical protein
Rv1949c	3	28	-2.311710	Conserved hypothetical protein
Rv1950c	5	26	-1.673474	Conserved hypothetical protein
Rv1951c	3	23	-2.090438	Conserved hypothetical protein
Rv1952	3	27	-3.127772	Possible antitoxin VapB14
Rv1953	2	27	-3.611202	Possible toxin VapC14
Rv1954c	4	29	-3.352723	Hypothetical protein
Rv1954A	3	26	-3.672347	Hypothetical protein
Rv1955	4	25	-3.564599	Possible toxin HigB
Rv1956	1	25	-2.243660	Possible antitoxin HigA
Rv1957	3	25	-2.253822	Hypothetical protein
Rv1958c	4	25	-1.794580	Hypothetical protein
Rv1959c	5	16	-0.656139	Possible toxin ParE1

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv1941	DHBDHDRGNASE	120	4e-35	2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase signature. Length = 261 Score = 120 bits (302), Expect = 4e-35 Identities = 73/257 (28%), Positives = 114/257 (44%), Gaps = 6/257 (2%) Query: 1 MNHPDLAGKVAIVTGAGAGIGLAVARRLADEGCHVLCADIDGD---AADAAATKIGCGAA 57 MN + GK+A +TGA GIG AVAR LA +G H+ D + + ++ A Sbjct: 1 MNAKGIEGKIAFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHAE 60 Query: 58 ACRVDVSDEQQIIAMVDACVAAFGGVDKLVANAGVVHLASLIDTTVEDFDRVIAINLRGA 117 A DV D I + G +D LV AGV+ + + E+++ ++N G Sbjct: 61 AFPADVRDSAAIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGV 120 Query: 118 WLCTKHAAPRMIERGGGAIVNLSSLAGQVAVGGTGAYGMSKAGIIQLSRITAAELRSSGI 177 + ++ + M++R G+IV + S V AY SKA + ++ EL I Sbjct: 121 FNASRSVSKYMMDRRSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLELAEYNI 180 Query: 178 RSNTLLPAFVDTPMQQTAMAMFDGA---LGAGGARSMIARLQGRMAAPEEMAGIVVFLLS 234 R N + P +T MQ + A +GA + ++A P ++A V+FL+S Sbjct: 181 RCNIVSPGSTETDMQWSLWADENGAEQVIKGSLETFKTGIPLKKLAKPSDIADAVLFLVS 240 Query: 235 DDASMITGTTQIADGGT 251 A IT DGG Sbjct: 241 GQAGHITMHNLCVDGGA 257
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv1944c	SECA	43	5e-07	SecA protein signature.
>SECA#SecA protein signature. Length = 901 Score = 42.6 bits (100), Expect = 5e-07 Identities = 17/55 (30%), Positives = 19/55 (34%), Gaps = 8/55 (14%) Query: 138 QEPDSPEARAEYAAYLTAHGDHDVMAWP--------PGRNQQCWCGSGHKYKKCC 184 Q E A+ D A GRN C CGSG KYK+C Sbjct: 843 QRRMEAERLAQMQQLSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCH 897
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv1946c	BLACTAMASEA	27	0.032	Beta-lactamase class A signature.
>BLACTAMASEA#Beta-lactamase class A signature. Length = 286 Score = 27.1 bits (60), Expect = 0.032 Identities = 11/57 (19%), Positives = 21/57 (36%) Query: 38 LINLSGIQCFARIEHVAHAQAHPFVVLVGKPAQHGARIGAVAGAILTGDVIVSHDGE 94 I L I A + HA P + +Q R+G + + +G + + + Sbjct: 3 YIRLCIISLLATLPLAVHASPQPLEQIKLSESQLSGRVGMIEMDLASGRTLTAWRAD 59
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv1950c	CHLAMIDIAOMP	27	0.005	Chlamydia major outer membrane protein signature.
>CHLAMIDIAOMP#Chlamydia major outer membrane protein signature. Length = 393 Score = 26.9 bits (59), Expect = 0.005 Identities = 9/12 (75%), Positives = 9/12 (75%) Query: 44 IAWEGAGGDGCD 55 I WEG GGD CD Sbjct: 38 ILWEGFGGDPCD 49
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv1954A	HTHFIS	26	0.019	FIS bacterial regulatory protein HTH signature.
>HTHFIS#FIS bacterial regulatory protein HTH signature. Length = 484 Score = 26.3 bits (58), Expect = 0.019 Identities = 8/24 (33%), Positives = 14/24 (58%) Query: 22 RATAGGMPVLVVIESGTGGDQMAR 45 R + +++ ESGTG + +AR Sbjct: 155 RLMQTDLTLMITGESGTGKELVAR 178
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv1957	SECBCHAPRONE	27	0.030	Bacterial protein-transport SecB chaperone protein ...
>SECBCHAPRONE#Bacterial protein-transport SecB chaperone protein signature. Length = 170 Score = 27.2 bits (60), Expect = 0.030 Identities = 11/24 (45%), Positives = 12/24 (50%) Query: 137 LYPYIREYVYDLTGRLALPPLTLE 160 L+PY RE V L R P L L Sbjct: 117 LFPYARELVSSLVNRGTFPALNLS 140

Rv1975

Rv1980c

Genomic Island

LocusTag	DNBias	CDNBias	%GCBias	Product
Rv1975	-2	13	-3.384896	Conserved hypothetical protein
Rv1976c	-1	14	-4.224928	Conserved hypothetical protein
Rv1977	0	13	-4.250780	Conserved protein
Rv1978	0	14	-5.016315	Conserved protein
Rv1979c	-1	15	-4.047327	Possible conserved permease
Rv1980c	-2	17	-3.365010	Immunogenic protein Mpt64 (antigen Mpt64/MPB64)

Rv2006

Rv2024c

Genomic Island

LocusTag	DNBias	CDNBias	%GCBias	Product
Rv2006	2	15	-2.276992	Probable trehalose-6-phosphate phosphatase OtsB1
Rv2007c	5	22	-2.718132	Ferredoxin FdxA
Rv2008c	3	19	-1.719366	Conserved hypothetical protein
Rv2009	3	21	0.277098	Antitoxin VapB15
Rv2010	1	16	0.468483	Toxin VapC15
Rv2011c	1	16	-0.117974	Conserved hypothetical protein, probable
Rv2012	0	15	-0.469216	Conserved hypothetical protein
Rv2013	0	14	-0.195108	Transposase
Rv2014	0	16	-1.034041	Transposase
Rv2015c	0	15	-1.828482	Conserved hypothetical protein
Rv2016	2	19	-2.534049	Hypothetical protein
Rv2017	1	18	-2.475315	Transcriptional regulatory protein
Rv2018	2	24	-1.777543	Conserved protein
Rv2019	1	24	-5.317079	Conserved protein
Rv2020c	-1	14	-3.170125	Conserved hypothetical protein
Rv2021c	-2	10	-2.479659	Transcriptional regulatory protein
Rv2022c	-2	12	-2.545281	Conserved protein
Rv2023c	0	10	-1.808746	Hypothetical protein
Rv2023A	1	9	-1.647958	Conserved hypothetical protein
Rv2024c	2	8	-0.399568	Conserved membrane protein

Rv2060

Rv2082

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
Rv2060	-1	17	3.526906	Possible conserved integral membrane protein
Rv2061c	-1	17	3.932884	Conserved protein
Rv2062c	0	18	4.394456	Cobalamin biosynthesis protein CobN
Rv2063	1	13	2.587704	Antitoxin MazE7
Rv2063A	1	13	2.251004	Possible toxin MazF7
Rv2064	1	14	2.063205	Precorrin-3B synthase CobG
Rv2065	1	13	0.930381	Precorrin-8X methylmutase CobH (aka precorrin
Rv2066	1	12	0.402646	Probable bifunctional protein, CobI-COBJ fusion
Rv2067c	0	15	-0.440906	Conserved protein
Rv2068c	2	12	1.892889	Class a beta-lactamase BlaC
Rv2069	2	13	2.183111	RNA polymerase sigma factor, ECF subfamily,
Rv2070c	1	9	2.281238	Precorrin-6X reductase CobK
Rv2071c	1	10	2.204332	Precorrin-3 methylase CobM (precorrin-4
Rv2072c	1	9	1.684946	Precorrin-6Y C(5,15)-methyltransferase
Rv2073c	1	11	1.299388	Probable shortchain dehydrogenase
Rv2074	2	14	0.096937	Possible pyridoxamine 5'-phosphate oxidase
Rv2075c	-1	13	-0.595665	Possible hypothetical exported or envelope
Rv2076c	-2	17	-2.111136	Conserved hypothetical protein
Rv2077c	-1	18	-1.860326	Possible conserved transmembrane protein
Rv2077A	2	18	-0.811162	Conserved hypothetical protein
Rv2078	2	16	0.050997	Conserved hypothetical protein
Rv2079	2	17	0.188127	Conserved hypothetical protein
Rv2080	2	21	0.733545	Lipoprotein LppJ
Rv2081c	2	22	1.198018	Conserved transmembrane protein
Rv2082	2	22	1.159061	Conserved hypothetical protein

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv2060	SURFACELAYER	30	0.003	Lactobacillus surface layer protein signature.
>SURFACELAYER#Lactobacillus surface layer protein signature. Length = 439 Score = 30.0 bits (67), Expect = 0.003 Identities = 14/25 (56%), Positives = 17/25 (68%) Query: 66 SLLITPAAAAARVVVAPVAAIATSV 90 +L I AAAAA + VAP+AA A V Sbjct: 4 NLRIVSAAAAALLAVAPIAATAMPV 28
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv2068c	BLACTAMASEA	320	e-112	Beta-lactamase class A signature.
>BLACTAMASEA#Beta-lactamase class A signature. Length = 286 Score = 320 bits (821), Expect = e-112 Identities = 94/273 (34%), Positives = 136/273 (49%), Gaps = 8/273 (2%) Query: 33 PASTTLPAGADLADRFAELERRYDARLGVYVPATGTTAAIE-YRADERFAFCSTFKAPLV 91 + A ++ E + R+G+ + + +RADERF STFK L Sbjct: 14 TLPLAVHASPQPLEQIKLSESQLSGRVGMIEMDLASGRTLTAWRADERFPMMSTFKVVLC 73 Query: 92 AAVLHQNPLT--HLDKLITYTSDDIRSISPVAQQHVQTGMTIGQLCDAAIRYSDGTAANL 149 AVL + L++ I Y D+ SPV+++H+ GMT+G+LC AAI SD +AANL Sbjct: 74 GAVLARVDAGDEQLERKIHYRQQDLVDYSPVSEKHLADGMTVGELCAAAITMSDNSAANL 133 Query: 150 LLADLGGPGGGTAAFTGYLRSLGDTVSRLDAEEPELNRDPPGDERDTTTPHAIALVLQQL 209 LLA +GGP A T +LR +GD V+RLD E ELN PGD RDTTTP ++A L++L Sbjct: 134 LLATVGGP----AGLTAFLRQIGDNVTRLDRWETELNEALPGDARDTTTPASMAATLRKL 189 Query: 210 VLGNALPPDKRALLTDWMARNTTGAKRIRAGFPADWKVIDKTGTGDYGRANDIAVVWSPT 269 + L + L WM + IR+ PA W + DKTG G+ G +A++ Sbjct: 190 LTSQRLSARSQRQLLQWMVDDRVAGPLIRSVLPAGWFIADKTGAGERGARGIVALLGPNN 249 Query: 270 GVPYVVAVMSDRAGGGYDAEPREALLAEAATCV 302 +V + R AE + + A + Sbjct: 250 KAERIVVIYL-RDTPASMAERNQQIAGIGAALI 281
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv2073c	DHBDHDRGNASE	42	1e-06	2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase signature. Length = 261 Score = 41.6 bits (97), Expect = 1e-06 Identities = 41/178 (23%), Positives = 59/178 (33%) Query: 9 VVIFGGRSQIGGELARRLAAGATMVLAARNADQLADQAAALRAAGAIAVHTREFDADDLA 68 I G IG +AR LA+ + A + ++ + A A D D A Sbjct: 11 AFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHAEAFPADVRDSA 70 Query: 69 AHGPLVASLVAEHGPIGTAVLAFGILGDQARAETDAAHAVAIVHTDYVAQVSLLTHLAAA 128 A + A + E GPI V G+L A + + ++ Sbjct: 71 AIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGVFNASRSVSKY 130 Query: 129 MRTAGRGSLVVFSSVAGIRVRRANYVYGSAKAGLDGFASGLADALHGTGVRLLIARPG 186 M GS+V S R + Y S+KA F L L +R I PG Sbjct: 131 MMDRRSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLELAEYNIRCNIVSPG 188
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv2082	PF03544	31	0.018	Gram-negative bacterial tonB protein
>PF03544#Gram-negative bacterial tonB protein Length = 243 Score = 30.7 bits (69), Expect = 0.018 Identities = 16/90 (17%), Positives = 24/90 (26%) Query: 379 APTPSPAPIAPPTTDNASAMTPIAPMVANGPPASPAPPAAAPAGPLPAYGADLRPPVTTP 438 A P P P+ P + P P P PV + Sbjct: 65 AVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKPVKKVEQPKRDVKPVESR 124 Query: 439 PATPPTPTGPISGAAVTPSSPAAGGSLMSP 468 PA+P T P + T ++ + Sbjct: 125 PASPFENTAPARPTSSTATAATSKPVTSVA 154

Rv2095c

Rv2106

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
Rv2095c	-2	11	3.020726	Proteasome accessory factor C PafC
Rv2096c	-2	13	3.207404	Proteasome accessory factor B PafB
Rv2097c	-1	15	3.399826	Proteasome accessory factor a PafA
Rv2098c	-1	13	4.236626	Conserved hypothetical protein
Rv2099c	-1	12	2.824972	Probable helicase HelZ
Rv2100	0	12	2.651619	Conserved hypothetical protein
Rv2101	1	12	1.827999	Possible toxin VapC37. Contains PIN domain.
Rv2102	1	14	-0.854069	Possible antitoxin VapB37
Rv2104c	1	16	-3.505515	Putative transposase for insertion sequence
Rv2106	1	14	-3.612324	Probable transposase

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv2100	PF05616	30	0.018	Neisseria meningitidis TspB protein
>PF05616#Neisseria meningitidis TspB protein Length = 501 Score = 30.5 bits (68), Expect = 0.018 Identities = 16/40 (40%), Positives = 20/40 (50%), Gaps = 2/40 (5%) Query: 308 ASALDAQPDPHLSGDEPPSRPLTPETTLFEALTPDPEPDP 347 A A +AQP P +S E P+ P P+PEPDP Sbjct: 320 AEAPNAQPLPEVSPAENPANNPAPNEN--PGTRPNPEPDP 357

Rv2142c

Rv2167c

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
Rv2142c	3	17	-1.677567	*Possible toxin ParE2
Rv2142A	4	15	-0.949592	Possible antitoxin ParD2
Rv2143	1	15	-0.983545	Conserved hypothetical protein
Rv2144c	-1	13	-0.966306	Probable transmembrane protein
Rv2145c	0	12	-0.662245	Diviva family protein Wag31
Rv2146c	2	11	0.013910	Possible conserved transmembrane protein
Rv2147c	1	9	0.786687	Conserved hypothetical protein
Rv2148c	1	10	2.427505	Conserved protein
Rv2149c	-1	11	2.760828	Conserved protein YfiH
Rv2150c	-2	11	3.116761	Cell division protein FtsZ
Rv2151c	-2	12	3.019169	Possible cell division protein FtsQ
Rv2152c	1	13	3.590127	Probable UDP-N-acetylmuramate-alanine ligase
Rv2153c	1	13	4.597556	Probable
Rv2154c	1	13	4.387468	FtsW-like protein FtsW
Rv2155c	2	13	4.985489	Probable UDP-N-acetylmuramoylalanine-D-glutamate
Rv2156c	1	14	5.116651	Probable
Rv2157c	2	16	6.383208	Probable
Rv2158c	1	17	7.751616	Probable
Rv2159c	0	13	5.463494	Conserved protein
Rv2160A	0	13	5.303338	Conserved hypothetical protein
Rv2160c	0	10	4.383646	Conserved hypothetical protein
Rv2161c	0	9	3.844756	Conserved protein
Rv2162c	0	9	2.714417	PE-PGRS family protein PE_PGRS38
Rv2163c	0	12	0.427057	Probable penicillin-binding membrane protein
Rv2164c	1	17	0.258071	Probable conserved proline rich membrane
Rv2165c	1	17	-1.274383	Conserved protein
Rv2166c	1	16	-1.400616	Conserved protein
Rv2167c	2	15	-1.418772	Probable transposase

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv2145c	RTXTOXIND	29	0.020	Gram-negative bacterial RTX secretion protein D signat...
>RTXTOXIND#Gram-negative bacterial RTX secretion protein D signature. Length = 478 Score = 29.0 bits (65), Expect = 0.020 Identities = 12/93 (12%), Positives = 28/93 (30%), Gaps = 10/93 (10%) Query: 124 ESDKMLADARANAEQILGEARHTADATVAEARQRADAMLADAQSRSEAQLRQAQEKADAL 183 E + +A + + A++ + ++ +LRQ + L Sbjct: 256 EQENKYVEAVNELRVYKSQLEQIESE-ILSAKEEYQLVTQLFKNEILDKLRQTTDNIGLL 314 Query: 184 Q-----ADAERKHSEIM----GTINQQRAVLEG 207 + ++ S I + Q + EG Sbjct: 315 TLELAKNEERQQASVIRAPVSVKVQQLKVHTEG 347
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv2151c	TYPE3IMSPROT	29	0.029	Type III secretion system inner membrane S protein ...
>TYPE3IMSPROT#Type III secretion system inner membrane S protein family signature. Length = 354 Score = 28.6 bits (64), Expect = 0.029 Identities = 11/41 (26%), Positives = 18/41 (43%), Gaps = 5/41 (12%) Query: 30 ESKDEPAE---HPEFEGPRRRARRERAERRAAQA--RATAI 65 E K E E PE + RR+ +E R + R++ + Sbjct: 220 EIKREYKEMEGSPEIKSKRRQFHQEIQSRNMRENVKRSSVV 260
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv2154c	PF03544	29	0.038	Gram-negative bacterial tonB protein
>PF03544#Gram-negative bacterial tonB protein Length = 243 Score = 29.2 bits (65), Expect = 0.038 Identities = 25/149 (16%), Positives = 45/149 (30%), Gaps = 7/149 (4%) Query: 368 FINIGYVIGLLPVTGLQLPLISAGGTSTAATLSLIGIIANAARHEPEAVAALRAGRDDKV 427 I+ V GLL + Q+ + A + T+ +A A P+AV Sbjct: 23 CIHGAVVAGLLYTSVHQVIELPAPAQPISVTM-----VAPADLEPPQAVQPPPE--PVVE 75 Query: 428 NRLLRLPLPEPYLPPRLEAFRDRKRANPQPAQTQPARKTPRTAPGQPARQMGLPPRPGSP 487 P+PEP + + + + P+P + + R +R Sbjct: 76 PEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKPVKKVEQPKRDVKPVESRPASPFENTAPA 135 Query: 488 RTADPPVRRSVHHGAGQRYAGQRRTRRVR 516 R + +G R R + Sbjct: 136 RPTSSTATAATSKPVTSVASGPRALSRNQ 164
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv2160A	HTHTETR	60	2e-13	TetR bacterial regulatory protein HTH signature.
>HTHTETR#TetR bacterial regulatory protein HTH signature. Length = 215 Score = 60.4 bits (146), Expect = 2e-13 Identities = 21/100 (21%), Positives = 43/100 (43%), Gaps = 1/100 (1%) Query: 2 PSADVGRQTRAQILRAAMDIASVKGLSGLSIGELAGRLGMSKSGLFRHFGAKEQLQLATV 61 + ++TR IL A+ + S +G+S S+GE+A G+++ ++ HF K L Sbjct: 4 KTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSEIW 63 Query: 62 EAAVSVFEAEVVAPAMAAPPG-VDRVRALMHAWVGYLERD 100 E + S + P + +R ++ + + Sbjct: 64 ELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVTE 103
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv2162c	cloacin	43	2e-06	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 43.2 bits (101), Expect = 2e-06 Identities = 38/119 (31%), Positives = 47/119 (39%), Gaps = 2/119 (1%) Query: 255 AGGNGSNGVTGVHGGNGGAGGAAGLIGNGGAGGDGGNGGLSNTGASGGAGGAGGAALIGN 314 +GG+G TG H +G G +G GG DG N GG+G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 315 GGDGGHGGNGGHGNSGGAGGAGGAGGAGGAGGHVGLIGNGGNGGAGGNGGNDNSSTLAD 373 G+GG GN G G+ G GG A A A G L G G A S+ +AD Sbjct: 62 HGNGGGNGNSGGGS--GTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAAIAD 118 Score = 35.5 bits (81), Expect = 5e-04 Identities = 36/88 (40%), Positives = 40/88 (45%), Gaps = 5/88 (5%) Query: 127 NGGPGGLLYGNGGNGGAGDTANPNGGNGGSAGLIGNGGAGGAGAATGAGGAGGNGGWLYG 186 NGGP GL G G + G+G ++ N GGS I GG G G GG GN G G Sbjct: 21 NGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH----GNGGGNGNSGGGSG 76 Query: 187 NGGPGGAAGLGTAGGVSPAGGAGGAAGL 214 GG A A G PA GA GL Sbjct: 77 TGGNLSAVAAPVAFGF-PALSTPGAGGL 103 Score = 35.1 bits (80), Expect = 7e-04 Identities = 34/93 (36%), Positives = 36/93 (38%), Gaps = 11/93 (11%) Query: 352 GNGGNGGAGGNGGNDNSSTLADAGSGGAGAAGGNGGLFYGNGGVGGRGGNGGFSSAGTSG 411 G G N GA GN N GG G GG G+G GG S +G Sbjct: 6 GRGHNTGAHSTSGNIN---------GGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHW 56 Query: 412 GDGGIGGAGGIGGLIGSGGGGGDGGNGGQAPTP 444 G G G GG G SGGG G GGN P Sbjct: 57 GGGSGHGNGGGNG--NSGGGSGTGGNLSAVAAP 87 Score = 35.1 bits (80), Expect = 8e-04 Identities = 37/120 (30%), Positives = 46/120 (38%), Gaps = 8/120 (6%) Query: 289 GGNGGLSNTGASGGAGGAGGAALIGNGGDGGHGGNGGHGNSGGAGGAGGA-GGAGGAGGH 347 GG+G NTGA +G NGG G G GG + G GG G+G H Sbjct: 3 GGDGRGHNTGAHSTSGNI-------NGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIH 55 Query: 348 VGLIGNGGNGGAGGNGGNDNSSTLADAGSGGAGAAGGNGGLFYGNGGVGGRGGNGGFSSA 407 G GNGG GN G + + + A G G GG+ G S+A Sbjct: 56 WGGGSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAA 115 Score = 34.3 bits (78), Expect = 0.001 Identities = 35/92 (38%), Positives = 38/92 (41%), Gaps = 4/92 (4%) Query: 144 GDTANPNGGNGGSAGLIGNGGAGGAGAATGAGGAGGNGGWLYGNGGPGGAAGLGTAGGVS 203 GD N G ++G I NGG G G GGA GW N GG +G G G Sbjct: 4 GDGRGHNTGAHSTSGNI-NGGPTGLG---VGGGASDGSGWSSENNPWGGGSGSGIHWGGG 59 Query: 204 PAGGAGGAAGLWGHGGAGGAGGSASGAPGAGG 235 G GG G G G G SA AP A G Sbjct: 60 SGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFG 91 Score = 34.3 bits (78), Expect = 0.001 Identities = 27/90 (30%), Positives = 36/90 (40%), Gaps = 2/90 (2%) Query: 228 SGAPGAGGAGGDGGRGGLLYGDGGAGGAGGNGSNGV--TGVHGGNGGAGGAAGLIGNGGA 285 SG G G G G + G G GG S+G + + GG G+ G G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 286 GGDGGNGGLSNTGASGGAGGAGGAALIGNG 315 G+GG G S G+ G + AA + G Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAAPVAFG 91 Score = 33.5 bits (76), Expect = 0.002 Identities = 24/80 (30%), Positives = 30/80 (37%) Query: 331 GAGGAGGAGGAGGAGGHVGLIGNGGNGGAGGNGGNDNSSTLADAGSGGAGAAGGNGGLFY 390 G G G GA G++ G G G + G+ SS G G GG + Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 391 GNGGVGGRGGNGGFSSAGTS 410 GNGG G G G + S Sbjct: 63 GNGGGNGNSGGGSGTGGNLS 82 Score = 32.0 bits (72), Expect = 0.006 Identities = 37/121 (30%), Positives = 43/121 (35%), Gaps = 15/121 (12%) Query: 394 GVGGRGGNGGFSSAGTSGGDGGIGGAGGIGGLIGSGGGGGDGGNGGQAPTPGNAGDGGAG 453 G GRG N G S G I G G GG +G + N GG+G Sbjct: 3 GGDGRGHNTGAHSTS-----------GNINGGPTGLGVGGGASDGSGWSSENNPWGGGSG 51 Query: 454 GNARLIGDGGRGGNGGEGGDGPPGVKGDGGNGGNGGNAVVIGNGG--NGGAGGFGIPVGS 511 G G G GG G G G G GGN V G GAGG + + + Sbjct: 52 SGIHWGGGSGHGNGGGNGNSG--GGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISA 109 Query: 512 G 512 G Sbjct: 110 G 110 Score = 32.0 bits (72), Expect = 0.006 Identities = 31/114 (27%), Positives = 37/114 (32%), Gaps = 13/114 (11%) Query: 210 GAAGLWGHGGAGGAGGSASGAPGAGGAGGDGGRGGLLYGDGGAGGAGGNGSNGVTGVHGG 269 G G + GA G+ +G P G GG G + G G G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 270 NGGAGGAAGLIGNGGAGGDGGNGGLSNTGASGGAG-------GAGGAALIGNGG 316 G G GG G G LS A G GAGG A+ + G Sbjct: 63 GNGGG------NGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAG 110 Score = 32.0 bits (72), Expect = 0.006 Identities = 35/100 (35%), Positives = 45/100 (45%), Gaps = 9/100 (9%) Query: 249 DGGAGGAGGNGSNGVTGVHGGNGGAGGAAGLIGNGGAGGDGGNGGLSNTGASGGAGGAGG 308 + GA GN + G TG+ G G + G+ N GG G+G G+ G GG Sbjct: 10 NTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGG--- 66 Query: 309 AALIGNGGDGGHGGNGGHGNSGGAGGAGG--AGGAGGAGG 346 GNG GG G GG+ ++ A A G A GAGG Sbjct: 67 ----GNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG 102 Score = 31.6 bits (71), Expect = 0.008 Identities = 36/104 (34%), Positives = 42/104 (40%), Gaps = 7/104 (6%) Query: 136 GNGGNGGAGDTA-NPNGGNGGSAGLIG-NGGAGGAGAATGAGGAGGNGGWLYGNGGPGGA 193 G G N GA T+ N NGG G G + G+G + GG G+G G G G Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNG 65 Query: 194 AGLGTAGGVSPAGGAGGAAGLWGHGGAGGAGGSASGAPGAGGAG 237 G G +GG S GG A G A PGAGG Sbjct: 66 GGNGNSGGGSGTGGNLSAV-----AAPVAFGFPALSTPGAGGLA 104 Score = 30.5 bits (68), Expect = 0.018 Identities = 36/106 (33%), Positives = 37/106 (34%), Gaps = 17/106 (16%) Query: 164 GAGGAGAATGAGGAGGNGGWLYGNGGPGGAAGLGTAGGVSPAGGAGGAAGLWGHGGAGGA 223 G G G TGA GN NGGP G LG GG S G WG G G Sbjct: 3 GGDGRGHNTGAHSTSGNI-----NGGPTG---LGVGGGASDGSGWSSENNPWGGGSGSGI 54 Query: 224 GGSASGAPGAGGAGGDGGRGGLLYGDGGAGGAGGNGSNGVTGVHGG 269 G GG G+ G G G GGN S V G Sbjct: 55 HWGGGSGHGNGGGNGNSG---------GGSGTGGNLSAVAAPVAFG 91 Score = 30.5 bits (68), Expect = 0.021 Identities = 30/98 (30%), Positives = 33/98 (33%), Gaps = 9/98 (9%) Query: 430 GGGGDGGNGGQAPTPGN--AGDGGAGGNARLIGDGGRGGNGGEGGDGPPGVKGDGGNGGN 487 GG G G N G T GN G G G G G G GG G+ Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 488 GGNAVVIGNGGNGGAGGFGIPVGSGGAGGSRGVLFGTP 525 GNGG G G G G + + V FG P Sbjct: 63 -------GNGGGNGNSGGGSGTGGNLSAVAAPVAFGFP 93
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv2164c	PERTACTIN	34	0.001	Pertactin signature.
>PERTACTIN#Pertactin signature. Length = 922 Score = 33.5 bits (76), Expect = 0.001 Identities = 20/68 (29%), Positives = 28/68 (41%), Gaps = 1/68 (1%) Query: 201 LVQDPDGNWVVVGTPKPADGVPPPPLNTKLPEDPPPPPKPAAVPLEVPVRVTPGPDDPAP 260 L + +G W +VG P P P + P PP P P + P P+ PAP Sbjct: 552 LAANGNGQWSLVGAKAPPAPKPAPQPGPQ-PGPQPPQPPQPPQPPQPPQPPQRQPEAPAP 610 Query: 261 PARSGPEV 268 +G E+ Sbjct: 611 QPPAGREL 618

Rv2266

Rv2274A

Genomic Island

LocusTag	DNBias	CDNBias	%GCBias	Product
Rv2266	-2	17	-4.107567	Probable cytochrome P450 124 Cyp124
Rv2267c	-2	20	-3.910103	Conserved hypothetical protein
Rv2268c	-1	20	-1.662910	Probable cytochrome P450 128 Cyp128
Rv2269c	1	25	-2.214867	Hypothetical protein
Rv2270	1	26	-2.048051	Probable lipoprotein LppN
Rv2271	0	27	-2.842957	Conserved hypothetical protein
Rv2272	2	21	-2.867387	Probable conserved transmembrane protein
Rv2273	1	21	-3.630063	Probable conserved transmembrane protein
Rv2274c	2	21	-3.711182	Possible toxin MazF8
Rv2274A	1	18	-3.248137	Possible antitoxin MazE8

Rv2294

Rv2308

Genomic Island

LocusTag	DNBias	CDNBias	%GCBias	Product
Rv2294	2	10	-1.610265	Probable aminotransferase
Rv2295	2	11	-1.626097	Conserved hypothetical protein
Rv2296	1	13	-1.468524	Probable haloalkane dehalogenase
Rv2297	0	13	-1.712341	Unknown protein
Rv2298	0	14	-1.998037	Conserved protein
Rv2299c	1	15	-2.343475	Probable chaperone protein HtpG (heat shock
Rv2300c	0	16	-0.488339	Conserved protein
Rv2301	0	15	0.010797	Probable cutinase Cut2
Rv2302	3	14	0.309566	Conserved protein
Rv2303c	2	12	0.583433	Probable antibiotic-resistance protein
Rv2304c	1	14	1.133975	Hypothetical protein
Rv2305	-1	16	-0.233380	Unknown protein
Rv2306A	1	25	-1.132043	Possible conserved membrane protein
Rv2306B	1	22	-2.296431	Possible conserved membrane protein
Rv2307c	1	23	-2.981362	Conserved hypothetical protein
Rv2307A	1	33	-5.403317	Hypothetical glycine rich protein
Rv2307B	1	27	-3.732749	Hypothetical glycine rich protein
Rv2307D	2	20	-1.497780	Hypothetical protein
Rv2308	3	21	-1.921750	Conserved hypothetical protein

Rv2324

Rv2356c

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
Rv2324	2	13	-0.066471	Probable transcriptional regulatory protein
Rv2325c	2	14	-0.326573	Conserved hypothetical protein
Rv2326c	2	13	-0.601887	Possible transmembrane ATP-binding protein ABC
Rv2327	2	20	-2.088906	Conserved protein
Rv2328	0	16	-2.856772	PE family protein PE23
Rv2329c	0	12	-2.545668	Probable nitrite extrusion protein 1 NarK1
Rv2330c	1	12	-1.745162	Probable lipoprotein LppP
Rv2331	2	13	-0.941707	Hypothetical protein
Rv2331A	1	13	-2.202054	Hypothetical protein
Rv2332	0	13	-2.099095	Probable [NAD] dependent malate oxidoreductase
Rv2333c	1	18	-3.305613	Integral membrane drug efflux protein Stp
Rv2334	1	23	-5.489575	Cysteine synthase a CysK1 (O-acetylserine
Rv2335	1	24	-4.430071	Probable serine acetyltransferase CysE (sat)
Rv2336	1	26	-4.970340	Hypothetical protein
Rv2337c	1	24	-4.125176	Hypothetical protein
Rv2338c	0	16	-3.172710	Possible molybdopterin biosynthesis protein
Rv2339	-1	11	-1.598079	Probable conserved transmembrane transport
Rv2340c	-1	9	1.794948	PE-PGRS family protein PE_PGRS39
Rv2341	0	10	0.948036	*Probable conserved lipoprotein LppQ
Rv2342	0	10	0.918554	Conserved hypothetical protein
Rv2343c	-1	9	0.998730	Probable DNA primase DnaG
Rv2344c	-3	9	-0.350337	Probable deoxyguanosine triphosphate
Rv2345	-1	8	-1.395633	Possible conserved transmembrane protein
Rv2346c	2	14	-3.038965	Putative ESAT-6 like protein EsxO (ESAT-6 like
Rv2347c	3	19	-1.853428	Putative ESAT-6 like protein EsxP (ESAT-6 like
Rv2348c	5	17	-2.813358	Hypothetical protein
Rv2349c	5	18	-2.897005	Probable phospholipase C 3 PlcC
Rv2350c	3	14	-2.486922	Membrane-associated phospholipase C 2 PlcB
Rv2351c	7	18	-2.802935	Membrane-associated phospholipase C 1 PlcA
Rv2352c	5	22	-3.145120	PPE family protein PPE38
Rv2353c	4	12	-4.208078	PPE family protein PPE39
Rv2354	0	14	-3.264091	Probable transposase for insertion sequence
Rv2355	0	13	-3.316972	Probable transposase
Rv2356c	1	14	-3.278230	PPE family protein PPE40

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv2333c	TCRTETB	150	3e-42	Tetracycline resistance protein TetB signature.
>TCRTETB#Tetracycline resistance protein TetB signature. Length = 458 Score = 150 bits (381), Expect = 3e-42 Identities = 84/398 (21%), Positives = 176/398 (44%), Gaps = 14/398 (3%) Query: 17 FMIFLDALIVNVALPDIQRSFAVGEDGLQWVVASYSLGMAVFIMSAATLADLDGRRRWYL 76 F L+ +++NV+LPDI F WV ++ L ++ L+D G +R L Sbjct: 24 FFSVLNEMVLNVSLPDIANDFNKPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLL 83 Query: 77 IGVSLFTLGSIACGLAPSIA-VLTTARGAQGLGAAAVSVTSLALVSAAFPEAKEKARAIG 135 G+ + GS+ + S +L AR QG GAAA + +V A + + + +A G Sbjct: 84 FGIIINCFGSVIGFVGHSFFSLLIMARFIQGAGAAAFPALVMVVV-ARYIPKENRGKAFG 142 Query: 136 IWTAIASIGTTTGPTLGGLLVDQWGWRSIFYVNLPMGALVLFLTLCYVEESCNERARRFD 195 + +I ++G GP +GG++ W + + +PM ++ L + + FD Sbjct: 143 LIGSIVAMGEGVGPAIGGMIAHYIHWS--YLLLIPMITIITVPFLMKLLKKEVRIKGHFD 200 Query: 196 LSGQLLFIVAVGALVYAVIEGPQIGWTSVQTIVMLWTAAVGCALFVWLERRSSNPMMDLT 255 + G +L V + + + + +++ + +FV R+ ++P +D Sbjct: 201 IKGIILMSVGIVFFMLFTT-------SYSISFLIVSVLSFL--IFVKHIRKVTDPFVDPG 251 Query: 256 LFRDTSYALAIATICTVFFAVYGMLLLTTQFLQNVRGYTPSVTG-LMILPFSAAVAIVSP 314 L ++ + + + +F V G + + +++V + + G ++I P + +V I Sbjct: 252 LGKNIPFMIGVLCGGIIFGTVAGFVSMVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGY 311 Query: 315 LVGHLVGRIGARVPILAGLCMLMLGLLMLIFSEHRSSALVLVGLGLCGSGVALCLTPITT 374 + G LV R G + G+ L + L F +S + + + G++ T I+T Sbjct: 312 IGGILVDRRGPLYVLNIGVTFLSVSFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVIST 371 Query: 375 VAMTAVPAERAGMASGIMSAQRAIGSTIGFAVLGSVLA 412 + +++ + AG +++ + G A++G +L+ Sbjct: 372 IVSSSLKQQEAGAGMSLLNFTSFLSEGTGIAIVGGLLS 409
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv2339	ACRIFLAVINRP	44	2e-06	Acriflavin resistance protein family signature.
>ACRIFLAVINRP#Acriflavin resistance protein family signature. Length = 1034 Score = 44.4 bits (105), Expect = 2e-06 Identities = 39/211 (18%), Positives = 82/211 (38%), Gaps = 30/211 (14%) Query: 159 ESVEAVRKIVANSTP--PEGIRTYVTGPAALFADQIAAGDRSMKLITGLTFAVITVLLLL 216 ++ +A++ +A P P+G++ F Q++ + L + + + L L Sbjct: 301 DTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFV-QLSIHEVVKTLFEAIMLVFLVMYLFL 359 Query: 217 VYRSIATTLLILPMVFIGLGATRGTIAFLGYHGMVGLSTFVVNILT----ALAIAAGTDY 272 +++ TL+ V + L T +A G + +N LT LAI D Sbjct: 360 --QNMRATLIPTIAVPVVLLGTFAILAAFG---------YSINTLTMFGMVLAIGLLVDD 408 Query: 273 AIFLVGRYQEARHIGQNREASFYTMYRGTANV---ILGSGLTIAGATYCLSFARLT---- 325 AI +V + R + +++ + + + ++G + ++ + A Sbjct: 409 AIVVVENVE--RVMMEDKLPPKEATEKSMSQIQGALVGIAMVLSAVF--IPMAFFGGSTG 464 Query: 326 -LFHTMGPPLAIGMLVSVAAALTLAPAIIAI 355 ++ + M +SV AL L PA+ A Sbjct: 465 AIYRQFSITIVSAMALSVLVALILTPALCAT 495 Score = 43.7 bits (103), Expect = 4e-06 Identities = 36/161 (22%), Positives = 63/161 (39%), Gaps = 15/161 (9%) Query: 774 GIAAVCLVFIVMLMITQSLIASLVIVGTVLLSLGTAFGLSVLIWQHFVGLQVH-WTIVAM 832 A+ LVF+VM + Q++ A+L+ V + L F + G ++ T+ M Sbjct: 344 LFEAIMLVFLVMYLFLQNMRATLIPTIAVPVVLLGTFAI-----LAAFGYSINTLTMFGM 398 Query: 833 SVIVLLAVGSDYNLLLV---SRFKEEVGAGLKTGIIRAMAGTGAVVTSAGLVFAFT---M 886 + + L V D +++V R E K ++M+ + +V + M Sbjct: 399 VLAIGLLV--DDAIVVVENVERVMMEDKLPPKEATEKSMSQIQGALVGIAMVLSAVFIPM 456 Query: 887 ASMAVSELRVIGQVGTTIGLGLLFDTLVVRSFMTPSIAALL 927 A S + Q TI + LV TP++ A L Sbjct: 457 AFFGGSTGAIYRQFSITIVSAMALSVLVALIL-TPALCATL 496
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv2340c	cloacin	34	0.001	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 33.9 bits (77), Expect = 0.001 Identities = 25/83 (30%), Positives = 37/83 (44%) Query: 179 HGSGATGAAGVADPGGSGAGVGSAAGNGTGAGSADAVGGAGTGRDIVGSVRGDGGVGMAS 238 H +GA +G + G +G GVG A +G+G S + G G+G I G G + Sbjct: 9 HNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGN 68 Query: 239 GDGGLSTGAAGASAEGGLMPGFG 261 G+ G +G G + FG Sbjct: 69 GNSGGGSGTGGNLSAVAAPVAFG 91 Score = 30.8 bits (69), Expect = 0.011 Identities = 26/100 (26%), Positives = 35/100 (35%), Gaps = 3/100 (3%) Query: 113 TGGTGTGQSRGAGGFGGVGQAGGKGWDGGPIGNGQ---VGEQHGAGQLGSTDGNPGVAGA 169 T G G G G GG G + P G G + G+G G+ Sbjct: 16 TSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNGNSGGGS 75 Query: 170 AHGSGVSASHGSGATGAAGVADPGGSGAGVGSAAGNGTGA 209 G +SA A G ++ PG G V +AG + A Sbjct: 76 GTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAA 115 Score = 29.7 bits (66), Expect = 0.024 Identities = 37/123 (30%), Positives = 45/123 (36%), Gaps = 3/123 (2%) Query: 208 GAGSADAVGGAGTGRDIVGSVRGDGGVGMASGDGGLSTGAAGASAEGGLMPGFGGAPWVG 267 G G G T +I G G G G AS G S+ G +GG G Sbjct: 4 GDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHG 63 Query: 268 GHWGLGGEG-HSGAIGGVGEQVAPAVATAPAVSP--ATTSAVAAESGSTPATKAQAMHAT 324 G G G SG G + AP PA+S A AV+ +G+ A A M A Sbjct: 64 NGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAAIADIMAAL 123 Query: 325 TNP 327 P Sbjct: 124 KGP 126
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv2345	GPOSANCHOR	41	1e-05	Gram-positive coccus surface protein anchor signature.
>GPOSANCHOR#Gram-positive coccus surface protein anchor signature. Length = 539 Score = 40.8 bits (95), Expect = 1e-05 Identities = 52/344 (15%), Positives = 109/344 (31%), Gaps = 39/344 (11%) Query: 224 VVDVDNAVRTSTNELALAIEEFGERRTAPFTQAVNNAKAALSQAFTVRQQLDDNTPETPA 283 +N ++ ++L+ + + + ++NAK L + + E A Sbjct: 62 FEIENNTLKLKNSDLSFNNKALKDHNDELTEE-LSNAKEKLRKNDKSLSEKASKIQELEA 120 Query: 284 QRRELLTRVIVSAAHADRELASQTEAFEKLRDLVINAPARLDLLTQQYVELTTRIGPTQQ 343 ++ +L + + A + +++ + E + + A L+ + + +T + Sbjct: 121 RKADL-EKALEGAMNFSTADSAKIKTLEAEKAALAARKADLEKALEGAMNFSTADSAKIK 179 Query: 344 RLAELHTEFDAAAMTSIAGNVTTATERLAFADRNISAARDLADQAVSGRQAGLVDAVRAA 403 L +A + L A +A + + A L Sbjct: 180 TLEAEKAALEARQ--------AELEKALEGAMNFSTADSAKIKTLEAEKAA-LAARKADL 230 Query: 404 ESALGQARALLDAVDSAATDIRHAVASLPAVVADIQTGIKRANQHLQQAQQPQTGRTGDL 463 E AL A A + + A+L A A+++ ++ A + Sbjct: 231 EKALEGAMNFSTADSAKIKTLEAEKAALEARQAELEKALEGAMNFSTADSAKIKTLEAE- 289 Query: 464 IAARDAAARALDRARGAADPLTAFDQLTKVDADLD---RLLATLAEEQATADRLNRSLEQ 520 AA +A L+ + + DLD L E + N+ E Sbjct: 290 KAALEAEKADLEHQSQVLNA-----NRQSLRRDLDASREAKKQLEAEHQKLEEQNKISEA 344 Query: 521 ALFTAESRVRAVSEYIDTRRGSIGPEARTRLAEAKRQLEAAHDR 564 + +++ +D R EAK+QLEA H + Sbjct: 345 SR-------QSLRRDLDASR------------EAKKQLEAEHQK 369
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv2353c	cloacin	36	2e-04	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 35.8 bits (82), Expect = 2e-04 Identities = 27/79 (34%), Positives = 34/79 (43%) Query: 24 GSGNIGSTNVGSGNIGDTNFGNGNNGNFNFGSGNTGSNNIGFGNTGSGNFGFGNTGNNNI 83 G G+ + SGNI G G G + GSG + NN G +GSG G +G+ N Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNG 65 Query: 84 GIGLTGDGQIGIGGLNSGS 102 G G G GG S Sbjct: 66 GGNGNSGGGSGTGGNLSAV 84 Score = 31.2 bits (70), Expect = 0.007 Identities = 27/88 (30%), Positives = 35/88 (39%) Query: 89 GDGQIGIGGLNSGSGNIGFGNSGTGNVGLFNSGTGNVGFGNSGTANTGFGNAGNVNTGFW 148 GDG+ G +S SGNI G +G G G + G+G N +G G +G Sbjct: 4 GDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHG 63 Query: 149 NGGSTNTGLANAGAGNTGFFDAGNYNFG 176 NGG +G G A FG Sbjct: 64 NGGGNGNSGGGSGTGGNLSAVAAPVAFG 91
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv2356c	cloacin	36	5e-04	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 35.8 bits (82), Expect = 5e-04 Identities = 29/91 (31%), Positives = 37/91 (40%) Query: 209 GVGNIGSLNLGSGNIGGTNVGSGNVGGTNLGSGNYGSLNWGSGNTGTGNAGSGNTGDYNP 268 G G+ + SGNI G G G GG + GSG N G +G+G G +G N Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNG 65 Query: 269 GSGNFGSGNFGSGNIGSLNVGSGNFGTLNLA 299 G G G+G S FG L+ Sbjct: 66 GGNGNSGGGSGTGGNLSAVAAPVAFGFPALS 96 Score = 33.9 bits (77), Expect = 0.002 Identities = 29/84 (34%), Positives = 36/84 (42%), Gaps = 9/84 (10%) Query: 229 GSGNVGGTNLGSGNYGSLNWGSGNTGTGNAGSGNTGDYNPGSGNFGSGNFGSGNIGSLNV 288 G G+ G + SGN G G G + GSG + + NP G GSG G G N Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNG 65 Query: 289 GSGNFGTLNLANGNNGDVNFGGGN 312 G NGN+G + GGN Sbjct: 66 GG---------NGNSGGGSGTGGN 80 Score = 33.1 bits (75), Expect = 0.004 Identities = 23/82 (28%), Positives = 32/82 (39%) Query: 465 AGDSNTGFANAGNVNTGFFNGGDINTGGFNGGNVNTGFGSALTQAGANSGFGNLGTGNSG 524 +G G + +G NGG G G + +G+ S G SG G G SG Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 525 WGNSDPSGTGNSGFFNTGNGNS 546 GN +G G GN ++ Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSA 83 Score = 30.8 bits (69), Expect = 0.016 Identities = 27/83 (32%), Positives = 34/83 (40%), Gaps = 4/83 (4%) Query: 255 TGNAGSGNTGDYNPGSGNFGSGNFGSGNIGSLNVGSGNFGTLNLANGNNGDVNFGGGNTG 314 +G G G+ + SGN G G G G + GSG N G +G GG +G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 315 DFNFGGGNNGTLNFGFGNTGSGN 337 N GG N G G+ GN Sbjct: 62 HGNGGGNGNS----GGGSGTGGN 80

Rv2421c

Rv2441c

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
Rv2421c	2	12	-0.196936	Probable nicotinate-nucleotide
Rv2422	1	13	0.345002	Hypothetical protein
Rv2423	1	12	0.334716	Hypothetical protein
Rv2424c	2	14	0.317167	Probable transposase
Rv2425c	2	13	-0.203713	Conserved hypothetical protein
Rv2426c	2	13	-1.269729	Conserved hypothetical protein
Rv2427c	0	12	-2.190887	Probable gamma-glutamyl phosphate reductase
Rv2427A	1	22	-3.852851	Alkyl hydroperoxide reductase C protein AhpC
Rv2428	1	23	-4.361522	Alkyl hydroperoxide reductase D protein AhpD
Rv2429	2	21	-3.491761	PPE family protein PPE41
Rv2430c	0	19	-4.626574	PE family protein PE25
Rv2431c	-1	18	-3.244156	Hypothetical protein
Rv2432c	-1	17	-3.018356	Hypothetical protein
Rv2433c	-2	13	-2.858130	Probable conserved transmembrane protein
Rv2435c	-1	12	-1.681036	Probable cyclase (adenylyl-or
Rv2436	0	10	0.865908	Ribokinase RbsK
Rv2437	1	10	0.518221	Conserved transmembrane protein
Rv2438c	1	12	0.348740	Glutamine-dependent NAD(+) synthetase NadE
Rv2438A	0	12	0.504325	Conserved hypothetical protein
Rv2439c	2	9	0.382081	Probable glutamate 5-kinase protein ProB
Rv2440c	3	9	-0.457381	Probable GTP1/Obg-family GTP-binding protein
Rv2441c	2	9	-0.968234	50S ribosomal protein L27 RpmA

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv2421c	LPSBIOSNTHSS	34	3e-04	Lipopolysaccharide core biosynthesis protein signat...
>LPSBIOSNTHSS#Lipopolysaccharide core biosynthesis protein signature. Length = 166 Score = 33.6 bits (77), Expect = 3e-04 Identities = 18/63 (28%), Positives = 26/63 (41%), Gaps = 4/63 (6%) Query: 3 GTFDPIHYGHLVAASEVADLFDLDEVVFVPSGQPWQKGRQVSAAEHRYLMTVIATASNPR 62 G+FDPI +GHL LF D+V P ++ + + + R A A P Sbjct: 7 GSFDPITFGHLDIIERGCRLF--DQVYVAVLRNPNKQP--MFSVQERLEQIAKAIAHLPN 62 Query: 63 FSV 65 V Sbjct: 63 AQV 65
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv2439c	CARBMTKINASE	40	8e-06	Bacterial carbamate kinase signature.
>CARBMTKINASE#Bacterial carbamate kinase signature. Length = 314 Score = 40.2 bits (94), Expect = 8e-06 Identities = 25/97 (25%), Positives = 38/97 (39%), Gaps = 6/97 (6%) Query: 156 DNDRLSALVAHLVGADALVLLSDIDGLYDCDPRKTADATFIPEVSGPADLDGVVAGRSSH 215 D D +A V AD ++L+D++G T ++ EV +L H Sbjct: 214 DKDLAGEKLAEEVNADIFMILTDVNGA--ALYYGTEKEQWLREVK-VEELRKYYE--EGH 268 Query: 216 LGTGGMASKVAAALLAADA-GVPVLLAPAADAATALA 251 G M KV AA+ + G ++A A AL Sbjct: 269 FKAGSMGPKVLAAIRFIEWGGERAIIAHLEKAVEALE 305

Rv2484c

Rv2492

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
Rv2484c	-1	9	3.175021	Possible triacylglycerol synthase
Rv2485c	4	12	6.628014	Probable carboxylesterase LipQ
Rv2486	5	14	6.468792	*Probable enoyl-CoA hydratase EchA14 (enoyl
Rv2487c	6	14	5.440959	PE-PGRS family protein PE_PGRS42
Rv2488c	4	14	4.771077	Probable transcriptional regulatory protein
Rv2489c	8	20	5.572478	Hypothetical alanine rich protein
Rv2490c	7	18	4.920927	PE-PGRS family protein PE_PGRS43
Rv2491	-1	17	-4.143500	Conserved hypothetical protein
Rv2492	-1	16	-3.222873	Hypothetical protein

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv2487c	cloacin	39	9e-05	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 38.5 bits (89), Expect = 9e-05 Identities = 30/86 (34%), Positives = 36/86 (41%), Gaps = 4/86 (4%) Query: 145 GFGQTGGSGGAAGLIGNGGNGGAGGTGAAGGAGGNGGWLWGNGGNGGVGGTSVAAGIGGA 204 G G G+ +G I NGG G G GGA GW N GG G+ + G G Sbjct: 6 GRGHNTGAHSTSGNI----NGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 205 GGNGGNAGLFGHGGAGGTGGAGLAGA 230 GNGG G G G G + +A Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAAP 87 Score = 38.2 bits (88), Expect = 1e-04 Identities = 28/98 (28%), Positives = 35/98 (35%) Query: 566 NGGSGGVGGAGGVGGAGGDGGNGGSGGNASTFGDENSIGGAGGTGGNGGNGANGGNGGAG 625 NGG G+G GG G G S G G G GG GN G G Sbjct: 21 NGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNGNSGGGSGTGGN 80 Query: 626 GIAGGAGGSGGFLSGAAGVSGADGIGGAGGAGGAGGAG 663 A A + GF + + +G + + GA A A Sbjct: 81 LSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAAIAD 118 Score = 36.6 bits (84), Expect = 3e-04 Identities = 26/70 (37%), Positives = 28/70 (40%) Query: 529 GGDGGSGGSSLGVGGVGGAGGVGGKGGASGMLIGNGGNGGSGGVGGAGGVGGAGGDGGNG 588 GG G G G G + GG SG I GG G G GG G GG G GGN Sbjct: 22 GGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNGNSGGGSGTGGNL 81 Query: 589 GSGGNASTFG 598 + FG Sbjct: 82 SAVAAPVAFG 91 Score = 35.5 bits (81), Expect = 8e-04 Identities = 35/106 (33%), Positives = 42/106 (39%), Gaps = 1/106 (0%) Query: 527 GVGGDGGSGGSSLGV-GGVGGAGGVGGKGGASGMLIGNGGNGGSGGVGGAGGVGGAGGDG 585 G G + G+ +S + GG G G GG SG N GG G G G G G+G Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNG 65 Query: 586 GNGGSGGNASTFGDENSIGGAGGTGGNGGNGANGGNGGAGGIAGGA 631 G G+ G S G S A G G G A I+ GA Sbjct: 66 GGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGA 111 Score = 35.1 bits (80), Expect = 0.001 Identities = 30/103 (29%), Positives = 45/103 (43%), Gaps = 1/103 (0%) Query: 227 LAGANGVNPTPGPAASTGDSPADVSGIGDQTGGDGGTGGHGTAGTPTGGTGGDGATATAG 286 ++G +G G +++G+ +G+G G G+G + P GG G G G Sbjct: 1 MSGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSG-WSSENNPWGGGSGSGIHWGGG 59 Query: 287 SGKATGGAGGDGGTAAAGGGGGNGGDGGVAQGDIASAFGGDGG 329 SG GG G+ G + GG + VA G A + G GG Sbjct: 60 SGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG 102 Score = 34.7 bits (79), Expect = 0.001 Identities = 39/122 (31%), Positives = 48/122 (39%), Gaps = 8/122 (6%) Query: 257 TGGDGGTGGHGTAGTPTGGTGGDGATATAGSGKATGGAGGDGGTAAAGGGGGNGGDGGVA 316 +GGDG GH T T G G T G A+ G+G GGG G+G G Sbjct: 2 SGGDGR--GHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGG 59 Query: 317 QGDIASAFGGDGGNGSDGVAAGSGGGSGGAGGGAFVHIATATSTGGSGGFGGNGAASAAS 376 G G+ G GS GG+ A A ST G+GG + +A A S Sbjct: 60 SGHGNGGGNGNSGGGS------GTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALS 113 Query: 377 GA 378 A Sbjct: 114 AA 115 Score = 34.3 bits (78), Expect = 0.002 Identities = 30/87 (34%), Positives = 37/87 (42%), Gaps = 1/87 (1%) Query: 604 GGAGGTGGNGGNGANGGNGGAGGIAGGAGGSGGFLSGAAGVSGADGIGGAGGAGGAGGAG 663 GA T GN NG G G GG + G+G S G GG G G GG G Sbjct: 11 TGAHSTSGNI-NGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNG 69 Query: 664 GSGGEAGAGGLTNGPGSPGVSGTEGMA 690 SGG +G GG + +P G ++ Sbjct: 70 NSGGGSGTGGNLSAVAAPVAFGFPALS 96 Score = 33.5 bits (76), Expect = 0.003 Identities = 30/91 (32%), Positives = 36/91 (39%), Gaps = 7/91 (7%) Query: 339 SGGGSGGAGGGAFVHIATATSTGGSGGFGGNGAASAASGADGGAGGAGGNGGAGGLLFGD 398 SGG G GA H + GG G G G AS DG + N GG G Sbjct: 2 SGGDGRGHNTGA--HSTSGNINGGPTGLGVGGGAS-----DGSGWSSENNPWGGGSGSGI 54 Query: 399 GGNGGAGGAGGIGGDGATGGPGGSGGNAGIA 429 GG+G G G + GG G G + +A Sbjct: 55 HWGGGSGHGNGGGNGNSGGGSGTGGNLSAVA 85 Score = 33.5 bits (76), Expect = 0.003 Identities = 27/101 (26%), Positives = 36/101 (35%) Query: 447 GGDGGKGGSGLGVGGAGGTGGAGGNGGAGGLLFGNGGNGGNAGAGGDGGAGVAGGVGGNG 506 GG G G G G+G + GG G GG G+ GG+G +G G GGN Sbjct: 22 GGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNGNSGGGSGTGGNL 81 Query: 507 GGGGTATFHEDPVAGVWAVGGVGGDGGSGGSSLGVGGVGGA 547 P GG+ +G S + + A Sbjct: 82 SAVAAPVAFGFPALSTPGAGGLAVSISAGALSAAIADIMAA 122 Score = 33.1 bits (75), Expect = 0.004 Identities = 27/78 (34%), Positives = 31/78 (39%) Query: 397 GDGGNGGAGGAGGIGGDGATGGPGGSGGNAGIARFDSPDPEAEPDVVGGKGGDGGKGGSG 456 G G N GA G G TG G G + G +P G G G G+G Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNG 65 Query: 457 LGVGGAGGTGGAGGNGGA 474 G G +GG G GGN A Sbjct: 66 GGNGNSGGGSGTGGNLSA 83 Score = 32.4 bits (73), Expect = 0.007 Identities = 23/61 (37%), Positives = 30/61 (49%) Query: 449 DGGKGGSGLGVGGAGGTGGAGGNGGAGGLLFGNGGNGGNAGAGGDGGAGVAGGVGGNGGG 508 +GG G G+G G + G+G + N GG GG +G G GG G +GG G GG Sbjct: 21 NGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNGNSGGGSGTGGN 80 Query: 509 G 509 Sbjct: 81 L 81 Score = 30.1 bits (67), Expect = 0.035 Identities = 33/100 (33%), Positives = 38/100 (38%), Gaps = 1/100 (1%) Query: 325 GGDGGNGSDGVAAGSGGGSGGAGGGAFVHIATATSTGGS-GGFGGNGAASAASGADGGAG 383 GGDG + G + SG +GG G A+ S S G G+ S G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 384 GAGGNGGAGGLLFGDGGNGGAGGAGGIGGDGATGGPGGSG 423 G GG G G G GGN A A G A PG G Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG 102
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv2490c	cloacin	38	4e-04	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 37.8 bits (87), Expect = 4e-04 Identities = 37/105 (35%), Positives = 45/105 (42%), Gaps = 5/105 (4%) Query: 1320 GGGGVGGHGGAGGDAGMNGGGGTGGQGGNGAAGGAGWSPDSDLKGFDGFDGGSGGAGGDG 1379 GG G G + GA +G GG TG G GA+ G+GWS +++ G GGSG G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWG-----GGSGSGIHWG 57 Query: 1380 GAGGAGGTQTGDGGDGGAGGLGGAGGVGGNGVDGFDINETTGRDG 1424 G G G GG+G G V GF T G G Sbjct: 58 GGSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG 102 Score = 34.7 bits (79), Expect = 0.004 Identities = 29/99 (29%), Positives = 40/99 (40%), Gaps = 3/99 (3%) Query: 427 GGAGGASGGAGARAGANGLAAGNDGPVSGGNGGKGGNGAHAPVAGGHGGN---GGAGGNG 483 G G + GA + +G G G + G G + + P GG G GG G+G Sbjct: 4 GDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHG 63 Query: 484 GLVGDGGAGGHGGDGAAGAGYADMTAIFLGSSGTPGEDG 522 G+G +GG G G + A A + TPG G Sbjct: 64 NGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG 102 Score = 34.3 bits (78), Expect = 0.005 Identities = 31/97 (31%), Positives = 41/97 (42%), Gaps = 1/97 (1%) Query: 1024 GAGGAGGAGGAGGSVSG-DGGAGGNGGAGGNGGVGASGGAGARGANGIDSIGGTGGAGGG 1082 G G GA G+++G G G GGA G + G+ GG G G G Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNG 65 Query: 1083 GGDGGAGGVGGHGGDGGVGGAAPSGTVGSHGTGGVGG 1119 GG+G +GG G GG+ A + + T G GG Sbjct: 66 GGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG 102 Score = 34.3 bits (78), Expect = 0.005 Identities = 29/108 (26%), Positives = 34/108 (31%) Query: 749 GGVGGGGAGGAGGDGGAGSSALGSGGNGGRGDAGQAGGAGGAGGAGGAGGSVSGDGGPGG 808 GG G G GA G + G GG G + GG+G + GG G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 809 KGGAGGAGGAGASGGGGGKGASGADSAEAVGGAGGKGGDGGVGGVGGD 856 G G G SG GG A A A G G + Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAG 110 Score = 33.9 bits (77), Expect = 0.005 Identities = 36/113 (31%), Positives = 40/113 (35%) Query: 729 GADGTLSGQPGEGSEANGGQGGVGGGGAGGAGGDGGAGSSALGSGGNGGRGDAGQAGGAG 788 G DG + N G G G GGA G S GG G G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 789 GAGGAGGAGGSVSGDGGPGGKGGAGGAGGAGASGGGGGKGASGADSAEAVGGA 841 G GG G G SG GG A A G A G G + + SA A+ A Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAA 115 Score = 33.9 bits (77), Expect = 0.006 Identities = 29/85 (34%), Positives = 32/85 (37%) Query: 1508 GQGGFGSTSGAHGKAGFGAPGGDGGDGGNGGHGGDGNGSFADAGDGGPGGNGGNGGLGGA 1567 G G G +GAH +G G G G G G G S + GG G GG G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 1568 GRDGGAPGGDGGDGGTGGSGGFGAP 1592 G GG GG G G AP Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAP 87 Score = 33.9 bits (77), Expect = 0.006 Identities = 33/101 (32%), Positives = 39/101 (38%) Query: 784 AGGAGGAGGAGGAGGSVSGDGGPGGKGGAGGAGGAGASGGGGGKGASGADSAEAVGGAGG 843 +GG G G S + +GGP G G GGA G+ S GG G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 844 KGGDGGVGGVGGDGGPGGDGGAGGAAPAGQVGSHGVGGVGG 884 G GG G GG G GG+ A A A + G GG Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG 102 Score = 33.5 bits (76), Expect = 0.008 Identities = 33/105 (31%), Positives = 43/105 (40%), Gaps = 6/105 (5%) Query: 911 GDPGAGGLGGLGGDSGNGTRAASGVDASDHGPGSGGNGGNGGNGAQASVAGGAGGNGGDG 970 G G G G SGN +G+ G G G + G+G + GG+G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGL-----GVGGGASDGSGWSSENNPWGGGSGSGIHWG 57 Query: 971 GNAGRVGDGGAGGNGGDGAAGANGANSGAPGSDAL-ALGQPGGNG 1014 G +G GG G +GG G N + AP + AL PG G Sbjct: 58 GGSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG 102 Score = 33.1 bits (75), Expect = 0.010 Identities = 40/140 (28%), Positives = 52/140 (37%), Gaps = 7/140 (5%) Query: 1053 NGGVGASGGAGARGANGIDSIGGTGGAGGGGGDGGAGGVGGHGGDGGVGGAAPSGTVGSH 1112 +GG G GA +G + G TG GGG G+G + GG G+ Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGI------- 54 Query: 1113 GTGGVGGDGGLGGAGGVGGAGGNGGIGITVGGAGGAGGNGGDPGAGGRGGLGGDSGNGTS 1172 GG G G GG G GG G GG V G G + +G ++ Sbjct: 55 HWGGGSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSA 114 Query: 1173 AANGVDASKHGPLTGGDGGV 1192 A + A+ GP G GV Sbjct: 115 AIADIMAALKGPFKFGLWGV 134 Score = 32.4 bits (73), Expect = 0.017 Identities = 35/105 (33%), Positives = 40/105 (38%), Gaps = 5/105 (4%) Query: 164 IGGAGGAGGAGAPGGTGGTGGWLAGGGGVGGMGGAGGGAGGAGGNAGLFGNGGAGGAGGA 223 + G G G T G G GVGG G G G G GG+ Sbjct: 1 MSGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGS 60 Query: 224 GGGAGGAGGNAGWFGHGGAGGVGGVGAAGANG----ATPGQDGAA 264 G G GG GN+G G G G + V A A G +TPG G A Sbjct: 61 GHGNGGGNGNSGG-GSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 32.0 bits (72), Expect = 0.023 Identities = 25/77 (32%), Positives = 29/77 (37%) Query: 1399 GLGGAGGVGGNGVDGFDINETTGRDGGDGGDGGYGGWGGAGGNGGAGGSAPAGEVGNRGV 1458 G G G G +IN G GG GW G G + G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 1459 GGDGGDGGSGGDAGNGG 1475 G GG+G SGG +G GG Sbjct: 63 GNGGGNGNSGGGSGTGG 79 Score = 32.0 bits (72), Expect = 0.024 Identities = 28/78 (35%), Positives = 29/78 (37%), Gaps = 4/78 (5%) Query: 1280 GNGGAGGAGGAGGAGGAFLGDGGNGGAGGQGGAGRGGS----PGGGGGVGGHGGAGGDAG 1335 G G G GA G G G GG G G S P GGG G GG Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 1336 MNGGGGTGGQGGNGAAGG 1353 NGGG GG+G G Sbjct: 63 GNGGGNGNSGGGSGTGGN 80 Score = 32.0 bits (72), Expect = 0.026 Identities = 30/90 (33%), Positives = 36/90 (40%), Gaps = 1/90 (1%) Query: 578 GAGGRGGDGGAGGAGGDAPAGRAGSQGVGGDGGAGGAGGAPGNGGSGGRGDMAFKDGDGG 637 G GRG + GA G+ G G GVGG G + N GG G G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGL-GVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 638 AGGDGGDPGAGGKGGAGGAGATEGVTGATG 667 G GG+ +GG G GG + A G Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAAPVAFG 91 Score = 31.6 bits (71), Expect = 0.031 Identities = 26/84 (30%), Positives = 29/84 (34%), Gaps = 1/84 (1%) Query: 1375 AGGDGGAGGAGGTQTGDGGDGGAGGLGGAGGVGGNGVDGFDINETTGRDGGDGGDGGYGG 1434 +GGDG G T +GG GLG GG +G N G G G G G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGG-ASDGSGWSSENNPWGGGSGSGIHWGGGS 60 Query: 1435 WGGAGGNGGAGGSAPAGEVGNRGV 1458 G GG G G V Sbjct: 61 GHGNGGGNGNSGGGSGTGGNLSAV 84 Score = 31.6 bits (71), Expect = 0.031 Identities = 30/103 (29%), Positives = 36/103 (34%), Gaps = 3/103 (2%) Query: 283 GGDGGAGGVGGNGGRGGWLLGNGGAGGVGGVGGAGGAGAAGGAGGAGATGINGPAGISAA 342 GGDG G + G NGG G+G GGA G Sbjct: 3 GGDGRGHNTGAHSTSGNI---NGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGG 59 Query: 343 GGDGGAGGNGGAGGNGGVGGAGGAGGSAGLLGYVGRAGDGGAG 385 G G GGNG +GG G GG A + G+ + G G Sbjct: 60 SGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG 102 Score = 31.2 bits (70), Expect = 0.036 Identities = 24/76 (31%), Positives = 29/76 (38%) Query: 522 GGNGGAGGAGGAGGAHAGDGGAGGAGGNGGAGGAGGNGAHGFNAVLVSDGGNGGDGGAGG 581 G N GA G G GG +G + N G + + GG G G GG Sbjct: 8 GHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGG 67 Query: 582 RGGDGGAGGAGGDAPA 597 G GG G GG+ A Sbjct: 68 NGNSGGGSGTGGNLSA 83 Score = 31.2 bits (70), Expect = 0.037 Identities = 27/82 (32%), Positives = 33/82 (40%) Query: 1236 LGGDGGAGGAGGKGGDAGDIGDGGDGGKGGDGAHGALGGLTVAGGNGGAGGAGGAGGAGG 1295 + G G G G +G+I G G G GA G + GG G+G G G Sbjct: 1 MSGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGS 60 Query: 1296 AFLGDGGNGGAGGQGGAGRGGS 1317 GGNG +GG G G S Sbjct: 61 GHGNGGGNGNSGGGSGTGGNLS 82 Score = 31.2 bits (70), Expect = 0.039 Identities = 27/84 (32%), Positives = 37/84 (44%) Query: 981 AGGNGGDGAAGANGANSGAPGSDALALGQPGGNGGQGDAGQAGGAGGAGGAGGAGGSVSG 1040 +GG+G GA+ + G G + G G + + GG G+G G SG Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 1041 DGGAGGNGGAGGNGGVGASGGAGA 1064 G GGNG +GG G G + A A Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVA 85 Score = 31.2 bits (70), Expect = 0.040 Identities = 32/101 (31%), Positives = 35/101 (34%), Gaps = 2/101 (1%) Query: 830 SGADSAEAVGGAGGKGGD--GGVGGVGGDGGPGGDGGAGGAAPAGQVGSHGVGGVGGDGG 887 SG D GA G+ GG G+G GG G GS GG G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 888 LGGAGGNGGDGGHGSDGGDGGDGGDPGAGGLGGLGGDSGNG 928 G GGNG GG GG+ P A G L G Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG 102 Score = 31.2 bits (70), Expect = 0.044 Identities = 25/75 (33%), Positives = 33/75 (44%), Gaps = 1/75 (1%) Query: 472 GHGGNGGAGGNGGLVGDG-GAGGHGGDGAAGAGYADMTAIFLGSSGTPGEDGGNGGAGGA 530 G G N GA G + G G GG + G+G++ + G SG+ GG G G Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNG 65 Query: 531 GGAGGAHAGDGGAGG 545 GG G + G G G Sbjct: 66 GGNGNSGGGSGTGGN 80

Rv2535c

Rv2547

Genomic Island

LocusTag	DNBias	CDNBias	%GCBias	Product
Rv2535c	-1	21	3.484989	Probable cytoplasmic peptidase PepQ
Rv2536	0	21	4.910452	Probable conserved transmembrane protein
Rv2537c	2	18	4.655003	3-dehydroquinate dehydratase AroD (AROQ)
Rv2538c	1	16	3.805167	3-dehydroquinate synthase AroB
Rv2539c	0	16	2.585762	Shikimate kinase AroK (SK)
Rv2540c	1	14	1.627698	Probable chorismate synthase AroF
Rv2541	1	16	-0.633118	Hypothetical alanine rich protein
Rv2542	0	16	-2.156467	Conserved hypothetical protein
Rv2543	0	23	-3.717657	Probable conserved lipoprotein LppA
Rv2544	1	23	-3.243997	Probable conserved lipoprotein LppB
Rv2545	3	19	-2.278096	Possible antitoxin VapB18
Rv2546	5	19	-2.278373	Possible toxin VapC18
Rv2547	3	15	-0.436459	Possible antitoxin VapB19

Rv2629

Rv2677c

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
Rv2629	3	15	2.170083	Conserved protein
Rv2630	5	18	2.851651	Hypothetical protein
Rv2631	5	17	2.553560	Conserved hypothetical protein
Rv2632c	4	17	3.203860	Conserved protein
Rv2633c	4	17	3.616057	Hypothetical protein
Rv2634c	6	16	4.262600	PE-PGRS family protein PE_PGRS46
Rv2635	3	16	0.328022	Hypothetical protein
Rv2636	1	16	0.121943	Conserved hypothetical protein
Rv2637	0	15	1.034976	Possible transmembrane protein DedA
Rv2638	3	15	0.481918	Conserved hypothetical protein
Rv2639c	2	15	-0.066798	Probable conserved integral membrane protein
Rv2640c	0	17	0.682490	Possible transcriptional regulatory protein
Rv2641	-1	18	0.769523	Cadmium inducible protein CadI
Rv2642	-1	19	0.794548	Possible transcriptional regulatory protein
Rv2643	0	19	0.617316	Probable arsenic-transport integral membrane
Rv2644c	1	24	0.157635	Hypothetical protein
Rv2645	1	17	0.835399	****Hypothetical protein
Rv2646	2	15	0.254059	Probable integrase
Rv2647	3	15	-0.000785	Hypothetical protein
Rv2648	2	15	0.179761	Probable transposase for insertion sequence
Rv2649	3	16	0.889773	Probable transposase for insertion sequence
Rv2650c	4	15	1.465326	Possible PhiRv2 prophage protein
Rv2651c	2	16	1.867496	Possible PhiRv2 prophage protease
Rv2652c	1	16	2.202886	Probable PhiRv2 prophage protein
Rv2653c	3	18	1.499208	Possible PhiRv2 prophage protein
Rv2654c	0	19	0.926632	Possible PhiRv2 prophage protein
Rv2655c	-1	21	-0.203905	Possible PhiRv2 prophage protein
Rv2656c	-1	22	-0.461024	Possible PhiRv2 prophage protein
Rv2657c	-1	25	-1.538461	Probable PhiRv2 prophage protein
Rv2658c	-1	27	-2.250355	Possible prophage protein
Rv2659c	0	27	-1.263970	Probable PhiRv2 prophage integrase
Rv2660c	3	24	-0.433847	Hypothetical protein
Rv2661c	4	21	0.680157	Hypothetical protein
Rv2662	2	22	0.807881	Hypothetical protein
Rv2663	0	22	1.364761	Hypothetical protein
Rv2664	-1	20	1.480806	Hypothetical protein
Rv2665	0	14	0.794852	Hypothetical arginine rich protein
Rv2666	0	16	0.634479	Probable transposase for insertion sequence
Rv2667	0	14	0.999144	Possible ATP-dependent protease ATP-binding
Rv2668	1	12	0.093496	Possible exported alanine and valine rich
Rv2669	2	11	-0.326271	GCN5-related N-acetyltransferase
Rv2670c	1	10	-0.072228	Conserved hypothetical protein
Rv2671	1	11	0.154249	Possible bifunctional enzyme riboflavin
Rv2672	0	12	1.408030	Possible secreted protease
Rv2673	-1	15	1.645305	Possible arabinofuranosyltransferase AftC
Rv2674	-1	13	2.818703	Probable peptide methionine sulfoxide reductase
Rv2675c	0	12	3.276070	Conserved hypothetical protein
Rv2676c	0	12	3.426037	Conserved protein
Rv2677c	-1	11	3.167503	Probable protoporphyrinogen oxidase HemY

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv2634c	cloacin	42	6e-06	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 42.4 bits (99), Expect = 6e-06 Identities = 42/111 (37%), Positives = 47/111 (42%), Gaps = 2/111 (1%) Query: 570 AGGVGGAGGEGLTDGAGTAEGGTGGLGGLGGVGGTGGMGGSGGVGGNGGAAGSLIGLGGG 629 +GG G G +G GG GLG GG + G G S GG +GS I GGG Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGG--ASDGSGWSSENNPWGGGSGSGIHWGGG 59 Query: 630 GGAGGVGGTGGIGGIGGAGGNGGAGGAGTTTGGGATIGGGGGTGGVGGAGG 680 G G GG G GG G GGN A A G A G G V + G Sbjct: 60 SGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAG 110 Score = 39.3 bits (91), Expect = 5e-05 Identities = 39/116 (33%), Positives = 47/116 (40%), Gaps = 12/116 (10%) Query: 607 MGGSGGVGGNGGAAGSLIGLGGGGGAGGVGGTGGIGGIGGAGGNGGAGGAGTTTGGGATI 666 M G G G N GA + + GG GVGG G + G+G + G Sbjct: 1 MSGGDGRGHNTGAHSTSGNINGGPTGLGVGG----------GASDGSGWSSENNPWGGGS 50 Query: 667 GGGGGTGGVGGAGGTGGTGGAGGTTGGSGGAGGLIGWAGAAGGTGAGGTGGQGGLG 722 G G GG G G GG G +GG +G G + A A G A T G GGL Sbjct: 51 GSGIHWGGGSGHGNGGGNGNSGGGSGTGGNLSAVA--APVAFGFPALSTPGAGGLA 104 Score = 37.0 bits (85), Expect = 3e-04 Identities = 29/82 (35%), Positives = 37/82 (45%), Gaps = 2/82 (2%) Query: 647 AGGNGGAGGAGTTTGGGATIGGGGGTGGVGGAGGTGGTGGAGGTTGGSGGAGGLIGWAGA 706 +GG+G G + G GG G G G G + G+G + GG+G I W G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLG--VGGGASDGSGWSSENNPWGGGSGSGIHWGGG 59 Query: 707 AGGTGAGGTGGQGGLGGQGGNG 728 +G GG G GG G GGN Sbjct: 60 SGHGNGGGNGNSGGGSGTGGNL 81 Score = 36.6 bits (84), Expect = 4e-04 Identities = 28/81 (34%), Positives = 32/81 (39%) Query: 496 GKGGQGHNTGVGDAFGGDGGIGGDGNGALGAAGGNGGTGGAGGNGGRGGMLIGNGGAGGA 555 G G+GHNTG G G GA+ G+G + GG G I GG G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 556 GGTGGTGGGGAAGFAGGVGGA 576 G GG G G GG A Sbjct: 63 GNGGGNGNSGGGSGTGGNLSA 83 Score = 36.2 bits (83), Expect = 5e-04 Identities = 28/76 (36%), Positives = 33/76 (43%) Query: 548 GNGGAGGAGGTGGTGGGGAAGFAGGVGGAGGEGLTDGAGTAEGGTGGLGGLGGVGGTGGM 607 G G GA T G GG G G G + G G + GG+G GG G G Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNG 65 Query: 608 GGSGGVGGNGGAAGSL 623 GG+G GG G G+L Sbjct: 66 GGNGNSGGGSGTGGNL 81 Score = 33.9 bits (77), Expect = 0.002 Identities = 29/84 (34%), Positives = 35/84 (41%), Gaps = 1/84 (1%) Query: 348 MPGAGGNGGNANWFGSGGAGGQGGTGLAGTNGVNPGSIANPNTGANGTDNSGNGNQTGGN 407 M G G G N + G G TGL G + GS + G SG+G GG Sbjct: 1 MSGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWG-GGSGSGIHWGGG 59 Query: 408 GGPGPAGGVGEAGGVGGQGGLGES 431 G G GG G +GG G GG + Sbjct: 60 SGHGNGGGNGNSGGGSGTGGNLSA 83 Score = 33.9 bits (77), Expect = 0.003 Identities = 30/83 (36%), Positives = 38/83 (45%), Gaps = 2/83 (2%) Query: 432 LDGNDGTGGKGGAGGTAGTDGGAGGAGGAGGIGETDGSAGGVATGGEGGDGATGGVDGGV 491 + G DG G GA T+G G G GG G +DGS G + G G+ G+ G Sbjct: 1 MSGGDGRGHNTGAHSTSGNINGGPTGLGVGG-GASDGS-GWSSENNPWGGGSGSGIHWGG 58 Query: 492 GGAGGKGGQGHNTGVGDAFGGDG 514 G G GG N+G G GG+ Sbjct: 59 GSGHGNGGGNGNSGGGSGTGGNL 81 Score = 33.5 bits (76), Expect = 0.004 Identities = 34/108 (31%), Positives = 42/108 (38%), Gaps = 2/108 (1%) Query: 470 AGGVATGGEGGDGATGG-VDGGVGGAGGKGGQGHNTGVGDAFGGDGGIGGDGNGALGAAG 528 +GG G G +T G ++GG G G GG +G GG G G G +G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 529 -GNGGTGGAGGNGGRGGMLIGNGGAGGAGGTGGTGGGGAAGFAGGVGG 575 GNGG G G G G + A A G GA G A + Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISA 109 Score = 33.1 bits (75), Expect = 0.005 Identities = 26/90 (28%), Positives = 34/90 (37%), Gaps = 3/90 (3%) Query: 390 TGANGTDNSGNGNQTGGNGGPGPAGGVGEAGGVGGQGGLGESLDGNDGTG---GKGGAGG 446 +G +G ++ + T GN GP G G G G E+ G+G GG G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 447 TAGTDGGAGGAGGAGGIGETDGSAGGVATG 476 G GG+G G A VA G Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAAPVAFG 91 Score = 32.8 bits (74), Expect = 0.005 Identities = 30/82 (36%), Positives = 33/82 (40%), Gaps = 3/82 (3%) Query: 549 NGGAGGAGGT--GGTGGGGAAGFAGGVGGAGGEGLTDGAGTAEG-GTGGLGGLGGVGGTG 605 N GA G GG G G G A G E G G+ G GG G G GG G Sbjct: 10 NTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNG 69 Query: 606 GMGGSGGVGGNGGAAGSLIGLG 627 GG G GGN A + + G Sbjct: 70 NSGGGSGTGGNLSAVAAPVAFG 91 Score = 32.0 bits (72), Expect = 0.009 Identities = 35/104 (33%), Positives = 44/104 (42%), Gaps = 10/104 (9%) Query: 255 GNGGFGGAGGLGAAGGVGGAASYFGTGGGG----GVGGDGAPGGDG-GAGPLLIGNGGVG 309 G+G G +G + G + G GGG G + P G G G+G G G G Sbjct: 4 GDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHG 63 Query: 310 GLGGAGAAGGNGGAGGMLLGDGGAGGQGGPAVAGVLGGMPGAGG 353 GG G +GG G GG L G PA++ PGAGG Sbjct: 64 NGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALS-----TPGAGG 102 Score = 31.6 bits (71), Expect = 0.014 Identities = 25/71 (35%), Positives = 31/71 (43%) Query: 389 NTGANGTDNSGNGNQTGGNGGPGPAGGVGEAGGVGGQGGLGESLDGNDGTGGKGGAGGTA 448 NTGA+ T + NG TG G G + G G + GG S G G G GG Sbjct: 10 NTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNG 69 Query: 449 GTDGGAGGAGG 459 + GG+G G Sbjct: 70 NSGGGSGTGGN 80 Score = 30.8 bits (69), Expect = 0.024 Identities = 20/78 (25%), Positives = 25/78 (32%) Query: 464 GETDGSAGGVATGGEGGDGATGGVDGGVGGAGGKGGQGHNTGVGDAFGGDGGIGGDGNGA 523 G+ G G + +G G+ G G + G G N G G GG Sbjct: 4 GDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHG 63 Query: 524 LGAAGGNGGTGGAGGNGG 541 G GN G G G Sbjct: 64 NGGGNGNSGGGSGTGGNL 81 Score = 30.1 bits (67), Expect = 0.036 Identities = 31/102 (30%), Positives = 36/102 (35%) Query: 197 GTGGAGGAAGATLVGGTGGVGGATGLIGSGGFGGAGGAAAGVGTTGGVGGSGGVGGVFGN 256 G G G GA G G +G G G+G ++ GG G GG G+ Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 257 GGFGGAGGLGAAGGVGGAASYFGTGGGGGVGGDGAPGGDGGA 298 G GG G G G GG S G PG G A Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 30.1 bits (67), Expect = 0.036 Identities = 31/96 (32%), Positives = 36/96 (37%), Gaps = 6/96 (6%) Query: 677 GAGGTGGTGGAGGTTGG-SGGAGGLIGWAGAAGGTGAGGTGGQGGLGGQGGNGGNGGTGA 735 G G G GA T+G +GG GL GA+ G+G G GG G GG Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWG-GGSGSGIHWGGGSG 61 Query: 736 TGGQGGDFALGGNGGAGGAGGSPGGSSGIQGNMGPP 771 G GG GN G G G + G P Sbjct: 62 HGNGGG----NGNSGGGSGTGGNLSAVAAPVAFGFP 93 Score = 30.1 bits (67), Expect = 0.039 Identities = 25/72 (34%), Positives = 32/72 (44%), Gaps = 2/72 (2%) Query: 120 NGANGADGTGAPGGPGGLLLGNGGNGGSG--APGQPGGAGGDAGLIGNGGTGGKGGDGLV 177 N + GGP GL +G G + GSG + P G G +G+ GG+G G G Sbjct: 10 NTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNG 69 Query: 178 GSGAAGGVGGRG 189 SG G GG Sbjct: 70 NSGGGSGTGGNL 81 Score = 30.1 bits (67), Expect = 0.045 Identities = 30/109 (27%), Positives = 41/109 (37%) Query: 167 GTGGKGGDGLVGSGAAGGVGGRGGWLLGNGGTGGAGGAAGATLVGGTGGVGGATGLIGSG 226 G G+G + S + GG G +G G + G+G ++ GG G G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 227 GFGGAGGAAAGVGTTGGVGGSGGVGGVFGNGGFGGAGGLGAAGGVGGAA 275 G GG G + G TGG + FG G G A + A Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGA 111
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv2668	FIMBRILLIN	30	0.003	Porphyromonas gingivalis: fimbrillin protein signature.
>FIMBRILLIN#Porphyromonas gingivalis: fimbrillin protein signature. Length = 348 Score = 30.4 bits (68), Expect = 0.003 Identities = 18/55 (32%), Positives = 20/55 (36%), Gaps = 5/55 (9%) Query: 97 NSFILATNFSFTGV-TPFADAYKPRPCDASDWL----DAALGNAPQGSIVRGGVY 146 S + + TG T F AY P DWL NAPQG V Y Sbjct: 174 ASLANSDDAYLTGSLTNFNGAYSPANYTHVDWLGRDYTEPSNNAPQGFYVLESTY 228
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv2669	SACTRNSFRASE	51	5e-11	Streptothricin acetyltransferase signature.
>SACTRNSFRASE#Streptothricin acetyltransferase signature. Length = 173 Score = 51.5 bits (123), Expect = 5e-11 Identities = 20/97 (20%), Positives = 34/97 (35%) Query: 43 EYLTDPRRAILTARHDGRIVGYAMLIRGDDRDVELSKLYLLPGYHGTGAAAALMHKVLAT 102 Y+ + +A + +G + + + + + Y G AL+HK + Sbjct: 58 SYVEEEGKAAFLYYLENNCIGRIKIRSNWNGYALIEDIAVAKDYRKKGVGTALLHKAIEW 117 Query: 103 AADWGALRVWLGVNQKNQRAQRFYAKTGFKINGTRTF 139 A + + L N A FYAK F I T Sbjct: 118 AKENHFCGLMLETQDINISACHFYAKHHFIIGAVDTM 154

Rv2731

Rv2741

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
Rv2731	2	10	-0.267892	Conserved alanine and arginine rich protein
Rv2732c	0	12	-1.752216	Probable conserved transmembrane protein
Rv2733c	1	12	-2.048251	Conserved hypothetical alanine, arginine-rich
Rv2734	1	18	-3.716532	Conserved hypothetical protein
Rv2735c	1	15	-0.682950	Conserved hypothetical protein
Rv2736c	2	16	1.075895	Regulatory protein RecX
Rv2737c	0	12	2.870716	RecA protein (recombinase A) [contains:
Rv2737A	3	12	5.463400	Conserved hypothetical cysteine rich protein
Rv2738c	3	11	5.225851	Conserved hypothetical protein
Rv2739c	2	11	4.235482	Possible alanine rich transferase
Rv2740	4	12	3.255539	Epoxide hydrolase
Rv2741	4	13	2.706810	PE-PGRS family protein PE_PGRS47

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv2731	PF05616	35	7e-04	Neisseria meningitidis TspB protein
>PF05616#Neisseria meningitidis TspB protein Length = 501 Score = 34.7 bits (79), Expect = 7e-04 Identities = 18/46 (39%), Positives = 21/46 (45%), Gaps = 1/46 (2%) Query: 6 PRSDDSSGSAPQPAATPVPRPGPRPGPRPVPRPTSYPVGAHPPSDP 51 PR D + GSA P A P+P P P P P P G P +P Sbjct: 311 PRPDLTPGSAEAPNAQPLPEVSPAENPANNPAPNENP-GTRPNPEP 355
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv2737A	2FE2SRDCTASE	38	4e-07	Ferric iron reductase signature.
>2FE2SRDCTASE#Ferric iron reductase signature. Length = 262 Score = 37.7 bits (87), Expect = 4e-07 Identities = 13/26 (50%), Positives = 16/26 (61%) Query: 32 DLTFRRRSCCLFYRVPAGGKCGDCPL 57 D RR+CC YR+P +CGDC L Sbjct: 236 DGLLVRRTCCQRYRLPDVQQCGDCTL 261
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv2741	cloacin	39	3e-05	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 39.3 bits (91), Expect = 3e-05 Identities = 37/113 (32%), Positives = 48/113 (42%), Gaps = 5/113 (4%) Query: 233 GVGGAALGGGAQAAGGNGGAGGVGGLFGAGGAGGAGGFSDTGGTGGAGGAGGLFGPGGGS 292 G G GA + GN G G G G + G+G S+ GG G+G +G G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 293 GGVGGFGDTGGTGGDGGSGGLFGVGGAGGHGGFGSAAGGDGGAGGAGGTVFGS 345 G GG G++GG G GG+ A FG A GAGG ++ Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVA-----FGFPALSTPGAGGLAVSISAG 110 Score = 35.5 bits (81), Expect = 6e-04 Identities = 29/78 (37%), Positives = 32/78 (41%), Gaps = 1/78 (1%) Query: 289 GGGSGGVGGFGDTGGTGGDGGSGGLFGVGGAGGHGGFGSAAGGDGGAGGAGGTVFGSGGA 348 G G G G T G +GG GL GGA G+ S GG G+G G G Sbjct: 4 GDGRGHNTGAHSTSGNI-NGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 349 GGAGGVATVAGHGGHGGN 366 G GG G G GGN Sbjct: 63 GNGGGNGNSGGGSGTGGN 80 Score = 34.3 bits (78), Expect = 0.001 Identities = 30/89 (33%), Positives = 36/89 (40%), Gaps = 1/89 (1%) Query: 372 GTGGAGGAGGFGGFGGDGGDGGIGGLVGSGGAGGSGGTGTLSGGRGGAGGNAGTFYGSG- 430 G G G G G+ G G VG G + GSG + + GG+G GSG Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 431 GAGGAGGESDNGDGGNGGVGGKAGLVGEG 459 G GG G S G G G + A V G Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFG 91 Score = 33.9 bits (77), Expect = 0.002 Identities = 38/115 (33%), Positives = 43/115 (37%), Gaps = 5/115 (4%) Query: 400 SGGAGGSGGTGTLSGGRGGAGGNAGTFYGSGGAGGAG-GESDNGDGGNGGVGGKAGLVGE 458 SGG G TG S GG G G G + G+G +N GG G G G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 459 GGNGGDGGATIAGKGGSGGNGGNAWLTGQGGNGGNAAFGKAGTGSVGVGGAGGLL 513 GNGG G GG G GGN G A G G + V + G L Sbjct: 62 HGNGGGNG----NSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGAL 112 Score = 33.5 bits (76), Expect = 0.002 Identities = 28/102 (27%), Positives = 37/102 (36%), Gaps = 2/102 (1%) Query: 260 GAGGAGGAGGFSDTGGTGGAGGAGGLFGPGGGSGGVGGFGDTGGTGGDGGSGGLFGVGGA 319 G G G G T G G G G GGG+ G+ G G G+ GG+ Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGL--GVGGGASDGSGWSSENNPWGGGSGSGIHWGGGS 60 Query: 320 GGHGGFGSAAGGDGGAGGAGGTVFGSGGAGGAGGVATVAGHG 361 G G G+ G G G + + A G ++T G Sbjct: 61 GHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG 102 Score = 33.5 bits (76), Expect = 0.002 Identities = 33/113 (29%), Positives = 41/113 (36%), Gaps = 5/113 (4%) Query: 160 GAAGLFGNGGAGGAGASNQAGNGGAGGNGGAG-GLIWGTAGTGGNGGFTTFLDAAGGAGG 218 G G N GA + G G G GGA G W + GG + + GG+G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 219 AGGAGGLFGAGGAGGVGGAALGGGAQAAG----GNGGAGGVGGLFGAGGAGGA 267 G G GG+G G + A G GAGG+ AG A Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAA 115 Score = 33.5 bits (76), Expect = 0.002 Identities = 21/72 (29%), Positives = 28/72 (38%) Query: 120 NGANGKPGTGQDGGAGGLLYGSGGNGGSGLAGSGQKGGNGGAAGLFGNGGAGGAGASNQA 179 N +GG GL G G + GSG + G G +G+ GG+G Sbjct: 10 NTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNG 69 Query: 180 GNGGAGGNGGAG 191 +GG G GG Sbjct: 70 NSGGGSGTGGNL 81 Score = 32.8 bits (74), Expect = 0.004 Identities = 35/105 (33%), Positives = 42/105 (40%), Gaps = 5/105 (4%) Query: 169 GAGGAGASNQAGNGGAGGNGGAGGLIWGTAGTGGNGGFTTFLDAAGGAGGAGGAGGLFGA 228 G G G + A + NGG GL G + G+G + GG+G GG G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 229 GGAGGVGGAALGGGAQAAGGNGGAGGVGGLFG--AGGAGGAGGFS 271 G GG G GG GGN A FG A GAGG + Sbjct: 63 GNGGGNGN---SGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 32.0 bits (72), Expect = 0.006 Identities = 30/103 (29%), Positives = 37/103 (35%), Gaps = 1/103 (0%) Query: 198 AGTGGNGGFTTFLDAAGGAGGAGGAGGLFGAGGAGGVGGAALGGGAQAAGGNGGAGGVGG 257 +G G G T +G G G+ G G + +G GG G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 258 LFGAGGAGGAGGFSDTGGTGGAGGAGGLFG-PGGGSGGVGGFG 299 GG G +GG S TGG A A FG P + G GG Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 32.0 bits (72), Expect = 0.006 Identities = 33/108 (30%), Positives = 41/108 (37%), Gaps = 5/108 (4%) Query: 326 GSAAGGDGGAGGAGGTVFGSGGAGGAGGVATVAGHGGHGGNAGLLYGTGGAGGAGGFGGF 385 G G + GA G + G G GG A+ G G N G+G GG G Sbjct: 4 GDGRGHNTGAHSTSGNINGGPTGLGVGGGAS-DGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 386 GGDGGDGGIGGLVGSGGAGGSGGTGTLSG----GRGGAGGNAGTFYGS 429 G GG+G GG G+GG + G GAGG A + Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAG 110 Score = 32.0 bits (72), Expect = 0.007 Identities = 32/112 (28%), Positives = 38/112 (33%), Gaps = 7/112 (6%) Query: 140 GSGGNGGSGLAGSGQKGGNGGAAGLFGNGGAGGAGASNQAGNGGAGGNGGAGGLIWGTAG 199 G G N G+ GG G G G + N GG+G GG G Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH--G 63 Query: 200 TGGNGGFTTFLDAAGGAGGAGGAGGLFG-----AGGAGGVGGAALGGGAQAA 246 GG G + GG A A FG GAGG+ + G AA Sbjct: 64 NGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAA 115 Score = 31.6 bits (71), Expect = 0.009 Identities = 29/103 (28%), Positives = 36/103 (34%), Gaps = 1/103 (0%) Query: 318 GAGGHGGFGSAAGGDGGAGGAGGTVFGSGGAGGAGGVATVAGHGGHGGNAGLLYGTGGAG 377 G G G A G G + GGA G ++ G G +G+ +G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 378 GAGGFGGFGGDGGDGGIGGLVGSGGAGGSGGTGTLSGGRGGAG 420 G GG G G GG G G L G + G GG Sbjct: 63 GNGGGNGNSG-GGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104

Rv2755c

Rv2769c

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
Rv2755c	1	12	-3.047036	Possible type I restriction/modification system
Rv2756c	2	12	-2.549061	Possible type I restriction/modification system
Rv2757c	2	15	-0.661542	Possible toxin VapC21
Rv2758c	2	15	0.188922	Possible antitoxin VapB21
Rv2759c	2	13	-0.910176	Possible toxin VapC42. Contains PIN domain.
Rv2760c	0	12	-0.837855	Possible antitoxin VapB42
Rv2761c	0	12	-0.878574	Possible type I restriction/modification system
Rv2762c	-1	14	-0.856494	Conserved hypothetical protein
Rv2763c	-1	14	-1.031031	Dihydrofolate reductase DfrA (DHFR)
Rv2764c	0	14	-0.619366	Probable thymidylate synthase ThyA (ts) (TSASE)
Rv2765	2	14	0.491579	Probable alanine rich hydrolase
Rv2766c	2	16	0.159253	Probable short-chain type
Rv2767c	3	18	0.409406	Possible membrane protein
Rv2768c	2	14	0.541638	PPE family protein PPE43
Rv2769c	2	14	0.448297	PE family protein PE27

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv2760c	adhesinb	28	0.006	Adhesin B signature.
>adhesinb#Adhesin B signature. Length = 310 Score = 27.5 bits (61), Expect = 0.006 Identities = 12/36 (33%), Positives = 17/36 (47%) Query: 31 DAVARRLSELDREDRARAEARRAAAEQTLRDLDKLL 66 +A+RLSE D ++ E A + L LDK Sbjct: 153 QNIAKRLSEKDPANKETYEKNLKAYVEKLSALDKEA 188
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv2766c	DHBDHDRGNASE	113	2e-32	2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase signature. Length = 261 Score = 113 bits (284), Expect = 2e-32 Identities = 78/261 (29%), Positives = 111/261 (42%), Gaps = 15/261 (5%) Query: 1 MTSLDLTGRTAIITGASRGIGLAIAQQLAAAGAHVV---LTARRQEAADEAAAQVGDRAL 57 M + + G+ A ITGA++GIG A+A+ LA+ GAH+ + E + A Sbjct: 1 MNAKGIEGKIAFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHAE 60 Query: 58 GVGAHAVDEDAARRCVDLTLERFGSVDILINNAGTNPAYGPLLEQDHARFAKIFDVNLWA 117 A D A G +DIL+N AG G + + F VN Sbjct: 61 AFPADVRDSAAIDEITARIEREMGPIDILVNVAGV-LRPGLIHSLSDEEWEATFSVNSTG 119 Query: 118 PLMWTSLVVTAWMGEHGGAVVNTASIGGMHQSPAMGMYNATKAALIHVTKQLALELSPR- 176 + V M G++V S +M Y ++KAA + TK L LEL+ Sbjct: 120 VFNASRSVSKYMMDRRSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLELAEYN 179 Query: 177 IRVNAICPGVVRTRLAEALWKDHE----------DPLAATIALGRIGEPADIASAVAFLV 226 IR N + PG T + +LW D + I L ++ +P+DIA AV FLV Sbjct: 180 IRCNIVSPGSTETDMQWSLWADENGAEQVIKGSLETFKTGIPLKKLAKPSDIADAVLFLV 239 Query: 227 SDAASWITGETMIIDGGLLLG 247 S A IT + +DGG LG Sbjct: 240 SGQAGHITMHNLCVDGGATLG 260

Rv2795c

Rv2801A

Genomic Island

LocusTag	DNBias	CDNBias	%GCBias	Product
Rv2795c	3	19	2.603284	Conserved hypothetical protein
Rv2796c	2	18	2.667165	Probable conserved lipoprotein LppV
Rv2797c	1	17	2.694175	Conserved hypothetical protein
Rv2798c	1	17	3.270112	Conserved hypothetical protein
Rv2799	2	16	3.029797	Probable membrane protein
Rv2800	1	13	3.395093	Possible hydrolase
Rv2801c	3	18	2.428215	Toxin MazF9
Rv2801A	2	17	2.722385	Possible antitoxin MazE9

Rv2813

Rv2828c

Genomic Island

LocusTag	DNBias	CDNBias	%GCBias	Product
Rv2813	4	20	-3.567047	Conserved hypothetical protein
Rv2814c	5	21	-5.089063	Probable transposase
Rv2815c	6	22	-5.306011	Probable transposase
Rv2816c	7	22	-5.528042	Conserved hypothetical protein
Rv2817c	7	20	-5.318585	Conserved hypothetical protein
Rv2818c	7	21	-5.034715	Hypothetical protein
Rv2819c	7	20	-4.509025	Hypothetical protein
Rv2820c	7	18	-3.135429	Hypothetical protein
Rv2821c	7	14	-2.600514	Conserved hypothetical protein
Rv2822c	4	13	-1.580178	Hypothetical protein
Rv2823c	4	13	-0.554981	Conserved protein
Rv2824c	2	13	2.299891	Hypothetical protein
Rv2825c	3	16	3.634536	Conserved hypothetical protein
Rv2826c	3	13	2.989660	Hypothetical protein
Rv2827c	2	16	4.001648	Hypothetical protein
Rv2828c	2	21	3.685422	Conserved hypothetical protein

Rv2884

Rv2894c

Genomic Island

LocusTag	DNBias	CDNBias	%GCBias	Product
Rv2884	3	14	-0.232587	Probable transcriptional regulatory protein
Rv2885c	2	11	-0.179899	Probable transposase
Rv2886c	3	13	0.730763	Probable resolvase
Rv2887	4	10	1.369632	Probable transcriptional regulatory protein
Rv2888c	3	9	1.760112	Probable amidase AmiC (aminohydrolase)
Rv2889c	2	9	2.450651	Probable elongation factor Tsf (EF-ts)
Rv2890c	0	9	2.529580	30S ribosomal protein S2 RpsB
Rv2891	0	9	3.867922	Conserved hypothetical protein
Rv2892c	-1	11	3.497069	PPE family protein PPE45
Rv2893	-2	11	3.685327	Possible oxidoreductase
Rv2894c	-2	13	3.035410	Probable integrase/recombinase XerC

Rv2947c

Rv2959c

Genomic Island

LocusTag	DNBias	CDNBias	%GCBias	Product
Rv2947c	0	25	-4.611643	Probable polyketide synthase Pks15
Rv2948c	1	23	-4.901506	P-hydroxybenzoyl-AMP ligase FadD22
Rv2949c	0	20	-4.853720	Chorismate pyruvate lyase
Rv2950c	0	16	-3.145962	Fatty-acid-AMP ligase FadD29 (fatty-acid-AMP
Rv2951c	2	16	-3.414874	Possible oxidoreductase
Rv2952	2	13	-3.533371	Possible methyltransferase (methylase)
Rv2953	2	14	-2.939932	Enoyl reductase
Rv2954c	3	17	-4.688124	Hypothetical protein
Rv2955c	4	17	-4.751311	Conserved protein
Rv2956	3	20	-5.835591	Conserved protein
Rv2957	1	15	-3.299044	Possible glycosyl transferase
Rv2958c	0	13	-3.146428	Possible glycosyl transferase
Rv2959c	-2	14	-3.778726	Possible methyltransferase (methylase)

Rv3010c

Rv3020c

Genomic Island

LocusTag	DNBias	CDNBias	%GCBias	Product
Rv3010c	1	14	3.146122	Probable 6-phosphofructokinase PfkA
Rv3011c	1	13	3.171340	Probable glutamyl-tRNA(GLN) amidotransferase
Rv3012c	1	11	2.674285	Probable glutamyl-tRNA(GLN) amidotransferase
Rv3013	-2	14	1.679366	Conserved protein
Rv3014c	-1	16	1.335733	DNA ligase [NAD dependent] LigA
Rv3015c	3	32	-0.178987	Conserved hypothetical protein
Rv3016	7	39	-2.092430	Probable lipoprotein LpqA
Rv3017c	11	48	-2.332614	ESAT-6 like protein EsxQ (TB12.9) (ESAT-6 like
Rv3018c	12	52	-2.111885	PPE family protein PPE46
Rv3018A	10	52	-1.224409	PE family protein PE27A
Rv3019c	4	26	-0.672231	Secreted ESAT-6 like protein EsxR (TB10.3)
Rv3020c	2	22	-0.164838	ESAT-6 like protein EsxS

Rv3046c

Rv3051c

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
Rv3046c	2	16	-4.020797	Conserved protein
Rv3047c	2	15	-4.434208	Hypothetical protein
Rv3048c	2	16	-4.489297	Ribonucleoside-diphosphate reductase (beta
Rv3049c	0	15	-3.853219	Probable monooxygenase
Rv3050c	1	13	-3.839952	Probable transcriptional regulatory protein
Rv3051c	0	12	-4.104124	Ribonucleoside-diphosphate reductase (alpha

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv3046c	PF06057	26	0.048	Type IV secretory pathway VirJ component
>PF06057#Type IV secretory pathway VirJ component Length = 243 Score = 26.0 bits (57), Expect = 0.048 Identities = 12/40 (30%), Positives = 18/40 (45%), Gaps = 5/40 (12%) Query: 70 RDEIAEFISEVTHHDAGPENIQR-VAGILAAAGWPLAGVD 108 + + F+S D G + + V GIL GWP+ G Sbjct: 50 KPPLVIFLS----GDGGWATLDKAVGGILQQQGWPVVGWS 85
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv3050c	HTHTETR	53	1e-10	TetR bacterial regulatory protein HTH signature.
>HTHTETR#TetR bacterial regulatory protein HTH signature. Length = 215 Score = 52.7 bits (126), Expect = 1e-10 Identities = 27/145 (18%), Positives = 47/145 (32%), Gaps = 2/145 (1%) Query: 23 RWREHRKKVRNEIVDAAFRAIDRLGPE-LSVRQIAEEAGTAKPKIYRHFTDKSDLLEAIG 81 + ++ ++ R I+D A R + G S+ +IA+ AG + IY HF DKSDL I Sbjct: 4 KTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSEIW 63 Query: 82 MRLRDMLWAAIFPSLDLATDSAREVIRRSVEEYVNLVDQHPNVLRVF-IQGRSAKQSEAT 140 + V+R + + + I + Sbjct: 64 ELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVTEERRRLLMEIIFHKCEFVGEM 123 Query: 141 VRTLNEGREITLAMAEMFNNELREM 165 R + L + L+ Sbjct: 124 AVVQQAQRNLCLESYDRIEQTLKHC 148

Rv3106

Rv3124

Genomic Island

LocusTag	DNBias	CDNBias	%GCBias	Product
Rv3106	0	20	-3.445891	NADPH:adrenodoxin oxidoreductase FprA
Rv3107c	0	29	-5.137650	Possible alkyldihydroxyacetonephosphate synthase
Rv3108	1	39	-8.899233	Hypothetical protein
Rv3109	1	37	-8.936958	Probable molybdenum cofactor biosynthesis
Rv3110	2	24	-5.049264	Probable pterin-4-alpha-carbinolamine
Rv3111	1	22	-5.322195	Probable molybdenum cofactor biosynthesis
Rv3112	0	18	-4.863981	Probable molybdenum cofactor biosynthesis
Rv3113	0	16	-4.004400	Possible phosphatase
Rv3114	-1	15	-3.822089	Conserved hypothetical protein
Rv3115	-1	15	-3.076631	Probable transposase
Rv3116	-2	19	-4.935985	Probable molybdenum cofactor biosynthesis
Rv3117	-1	17	-3.507305	Probable thiosulfate sulfurtransferase CysA3
Rv3118	-3	23	-2.441617	Conserved hypothetical protein SseC1
Rv3119	-1	28	-4.345504	Probable molybdenum cofactor biosynthesis
Rv3120	-2	30	-2.862690	Conserved hypothetical protein
Rv3121	-2	32	-3.490963	Probable cytochrome P450 141 Cyp141
Rv3122	-2	29	-2.600491	Hypothetical protein
Rv3123	-2	24	-3.226400	Hypothetical protein
Rv3124	-2	23	-3.700222	Transcriptional regulatory protein MoaR1

Rv3171c

Rv3190c

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
Rv3171c	2	15	0.808441	Possible non-heme haloperoxidase Hpx
Rv3172c	2	16	-0.112830	Hypothetical protein
Rv3173c	2	14	0.247120	Probable transcriptional regulatory protein
Rv3174	3	13	-0.431042	Probable short-chain dehydrogenase/reductase
Rv3175	2	14	-0.046652	Possible amidase (aminohydrolase)
Rv3176c	3	14	-1.112938	Probable epoxide hydrolase MesT (epoxide
Rv3177	2	13	-1.847999	Possible peroxidase (non-haem peroxidase)
Rv3178	3	14	-0.880026	Conserved hypothetical protein
Rv3179	4	13	-0.519786	Conserved protein
Rv3180c	3	21	-0.134196	Hypothetical alanine rich protein
Rv3181c	2	19	-0.711156	Conserved protein
Rv3182	4	24	-0.986457	Conserved hypothetical protein
Rv3183	5	24	-0.591639	Possible transcriptional regulatory protein
Rv3184	4	26	-1.065340	Probable transposase for insertion sequence
Rv3185	3	23	-2.316897	Probable transposase
Rv3186	3	22	-2.768652	Probable transposase for insertion sequence
Rv3187	3	20	-2.834686	Probable transposase
Rv3188	2	18	-2.477560	Conserved hypothetical protein
Rv3189	0	12	-3.368618	Conserved hypothetical protein
Rv3190c	0	10	-3.316499	Hypothetical protein

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv3173c	HTHTETR	89	2e-24	TetR bacterial regulatory protein HTH signature.
>HTHTETR#TetR bacterial regulatory protein HTH signature. Length = 215 Score = 89.3 bits (221), Expect = 2e-24 Identities = 42/169 (24%), Positives = 67/169 (39%), Gaps = 12/169 (7%) Query: 10 PPRRGGRGARQRILKAAAELFYCEGINATGVELIANKASVSKRTLYQHFPSKSALVEEYL 69 ++ + RQ IL A LF +G+++T + IA A V++ +Y HF KS L E Sbjct: 4 KTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSEIW 63 Query: 70 RGLRQAAGEA-----DKMPKASNATPRERLLALFDRPNRGDGR--MRGCPFHNAAVEAAG 122 GE K P + RE L+ + + + R + FH E G Sbjct: 64 ELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVTEERRRLLMEIIFHKC--EFVG 121 Query: 123 EMPGV---ERIVHSHKRDYIKGLARLAREAGAAHPRSLGNQLAVLFEGA 168 EM V +R + D I+ + EA + + A++ G Sbjct: 122 EMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMTRRAAIIMRGY 170
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv3174	DHBDHDRGNASE	63	5e-14	2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase signature. Length = 261 Score = 63.1 bits (153), Expect = 5e-14 Identities = 51/189 (26%), Positives = 83/189 (43%), Gaps = 15/189 (7%) Query: 7 RTVLVTGANRGMGREYVAQLLGRKVAKVYAATRNPLAIDVSDPRVIP-------LQLDVT 59 + +TGA +G+G E VA+ L + A + A NP ++ + DV Sbjct: 9 KIAFITGAAQGIG-EAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHAEAFPADVR 67 Query: 60 DAVSVAE-AADLATDVG---ILINNAGISRASSVLDKDTSALRGELETNLFGPLALASAF 115 D+ ++ E A + ++G IL+N AG+ R + N G + + Sbjct: 68 DSAAIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGVFNASRSV 127 Query: 116 ADRI-AERSGAIVNVSSVLAWLP-LGMS-YGVSKAAMWSATESMRIELAPRGVQVVGVYV 172 + + RSG+IV V S A +P M+ Y SKAA T+ + +ELA ++ V Sbjct: 128 SKYMMDRRSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLELAEYNIRCNIVSP 187 Query: 173 GLVDTDMGR 181 G +TDM Sbjct: 188 GSTETDMQW 196

Rv3338

Rv3388

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
Rv3338	5	14	-2.545656	Conserved hypothetical protein
Rv3339c	6	14	-0.966511	Probable isocitrate dehydrogenase [NADP] Icd1
Rv3340	10	19	2.779484	Probable O-acetylhomoserine sulfhydrylase MetC
Rv3341	11	20	2.899610	Probable homoserine O-acetyltransferase MetA
Rv3342	10	17	0.893963	Possible methyltransferase (methylase)
Rv3343c	10	17	0.794661	PPE family protein PPE54
Rv3344c	10	19	3.199528	PE-PGRS family protein PE_PGRS49
Rv3345c	10	15	0.945335	PE-PGRS family protein PE_PGRS50
Rv3346c	8	14	-1.238247	Conserved transmembrane protein
Rv3347c	8	14	-1.180532	PPE family protein PPE55
Rv3348	8	14	-0.497492	Probable transposase
Rv3349c	8	14	-0.448566	Probable transposase
Rv3350c	8	15	-0.647955	PPE family protein PPE56
Rv3351c	0	19	1.039976	Conserved hypothetical protein
Rv3352c	0	20	1.507530	Possible oxidoreductase
Rv3353c	-1	15	0.382426	Conserved hypothetical protein
Rv3354	0	13	0.830967	Conserved hypothetical protein
Rv3355c	2	15	0.429387	Probable integral membrane protein
Rv3356c	1	14	0.196785	Probable bifunctional protein FolD:
Rv3357	1	14	-0.934319	Antitoxin RelJ
Rv3358	0	13	-0.766159	Toxin RelK
Rv3359	0	13	-0.127468	Possible oxidoreductase
Rv3360	0	10	0.871647	Conserved hypothetical protein
Rv3361c	-2	10	1.230260	Conserved protein
Rv3362c	1	11	3.492255	Probable ATP/GTP-binding protein
Rv3363c	1	12	3.815775	Conserved hypothetical protein
Rv3364c	0	14	3.910305	Conserved protein
Rv3365c	-1	15	4.341340	Conserved protein
Rv3366	0	16	4.228284	Probable tRNA/rRNA methylase SpoU (tRNA/rRNA
Rv3367	0	15	4.151671	PE-PGRS family protein PE_PGRS51
Rv3368c	-1	15	2.922217	Possible oxidoreductase
Rv3369	-2	14	2.929624	Conserved protein
Rv3370c	-1	13	2.577348	Probable DNA polymerase III (alpha chain) DnaE2
Rv3371	1	16	0.569158	Possible triacylglycerol synthase
Rv3372	0	21	-2.554969	Trehalose 6-phosphate phosphatase OtsB2
Rv3373	4	29	-6.195096	Probable enoyl-CoA hydratase EchA18 (enoyl
Rv3374	1	28	-5.840353	Probable enoyl-CoA hydratase (fragment) EchA18.1
Rv3375	1	25	-5.476062	Probable amidase AmiD (acylamidase) (acylase)
Rv3376	1	29	-6.609995	Conserved hypothetical protein
Rv3377c	0	28	-6.423126	Halimadienyl diphosphate synthase
Rv3378c	-2	26	-4.760431	Diterpene synthase
Rv3379c	-1	21	-2.121729	Probable 1-deoxy-D-xylulose 5-phosphate synthase
Rv3380c	1	22	-1.792685	Probable transposase
Rv3381c	2	28	-1.427989	Probable transposase for insertion sequence
Rv3382c	1	21	-1.241136	Probable LYTB-related protein LytB1
Rv3383c	4	17	4.819016	Possible polyprenyl synthetase IdsB (polyprenyl
Rv3384c	4	20	6.376818	Possible toxin VapC46. Contains PIN domain.
Rv3385c	5	18	5.411336	Possible antitoxin VapB46
Rv3386	4	15	4.690265	Possible transposase
Rv3387	3	17	3.903543	Possible transposase
Rv3388	3	16	3.919443	PE-PGRS family protein PE_PGRS52

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv3343c	cloacin	32	0.036	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 32.0 bits (72), Expect = 0.036 Identities = 28/79 (35%), Positives = 34/79 (43%), Gaps = 7/79 (8%) Query: 1971 GAGNVGGFNVGGGNIGGNNVGLGNVGWGNFGLG-NSGLTP--GLMGLGNIGFGNAGSYNF 2027 G G+ G + GNI G GLG G + G G +S P G G G G +G N Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNG 65 Query: 2028 GLANMGVGNIGFANTGSGN 2046 G G GN G + GN Sbjct: 66 G----GNGNSGGGSGTGGN 80 Score = 32.0 bits (72), Expect = 0.036 Identities = 29/106 (27%), Positives = 40/106 (37%), Gaps = 4/106 (3%) Query: 193 NVGLFNAGSGNVGSYNVGAGNVGSYNVGGGNIGGNNVGLGNVGWGNFGLGNSGLTPGLMG 252 N G + SGN+ G G G + G G NN G G G G SG G Sbjct: 10 NTGAHST-SGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGN 68 Query: 253 LGNIGFGNAGSYNFG--LANMGVGNIGFANTGSGNFGIGLTGDNLT 296 GN G G+ N A + G + G+G + ++ L+ Sbjct: 69 -GNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALS 113 Score = 31.6 bits (71), Expect = 0.043 Identities = 26/94 (27%), Positives = 37/94 (39%), Gaps = 3/94 (3%) Query: 1842 GNTTGAPSSGFFNSGAGGVSGFGNVGAMVSGGWNQAPSALLGGGSGVFNAGTLHSGVLNF 1901 GN G P+ GA SG+ + GG GGGSG N G + Sbjct: 18 GNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHW--GGGSGHGNGGGNGNSGGGS 75 Query: 1902 GSGMSGLFNTSVLGLGAPALVS-GLGSVGQQLSG 1934 G+G + + + G PAL + G G + +S Sbjct: 76 GTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISA 109
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv3344c	cloacin	34	0.001	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 34.3 bits (78), Expect = 0.001 Identities = 30/99 (30%), Positives = 37/99 (37%), Gaps = 1/99 (1%) Query: 174 GAGGSGGNGGAGGNATGSGGKGGAGGNGGDGS-FGATSGPASIGVTGAPGGNGGKGGAGG 232 G G + G GN G G GG DGS + + + P G GG G G Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNG 65 Query: 233 SNPNGSGGDGGKGGNGGAGGNGGSIGANSGIVGGSGGAG 271 SGG G GGN A + G + G+GG Sbjct: 66 GGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 33.9 bits (77), Expect = 0.001 Identities = 31/80 (38%), Positives = 36/80 (45%), Gaps = 2/80 (2%) Query: 121 TGGSGSGIGGGAGGNGGN--GGAGGTGVVLGGKGGDGGNGDHGGPATNPGSGSRGGAGGS 178 +GG G G GA GN GG G GV G G G + ++ GSG G G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 179 GGNGGAGGNATGSGGKGGAG 198 GNGG GN+ G G GG Sbjct: 62 HGNGGGNGNSGGGSGTGGNL 81 Score = 33.9 bits (77), Expect = 0.001 Identities = 36/121 (29%), Positives = 41/121 (33%), Gaps = 13/121 (10%) Query: 13 GAGGNGGAGSAGNGGAGGAGGNGGAGGNGGGGDAGNAGSGGNGGKGGDGVGPGSTGGAGG 72 G G G A + GG G G GG D S N GG G G GG+G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 73 KGGAGANGGSSNGNARGGNAGNGGHGGAGGSGDTGGAGGAGGQGGFGGTGGSGSGIGGGA 132 G GGN +GG G GG+ A A G G G + A Sbjct: 63 GNG-------------GGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISA 109 Query: 133 G 133 G Sbjct: 110 G 110 Score = 33.9 bits (77), Expect = 0.001 Identities = 27/79 (34%), Positives = 35/79 (44%), Gaps = 1/79 (1%) Query: 69 GAGGKGGAGANGGSSNGNARGGNAGNGGHGGAG-GSGDTGGAGGAGGQGGFGGTGGSGSG 127 G G GA + G+ NG G G G G+G S + GG+G +GG G G+G Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNG 65 Query: 128 IGGGAGGNGGNGGAGGTGV 146 G G G G G + V Sbjct: 66 GGNGNSGGGSGTGGNLSAV 84 Score = 33.1 bits (75), Expect = 0.003 Identities = 28/90 (31%), Positives = 31/90 (34%), Gaps = 4/90 (4%) Query: 240 GDGGKGGNGGAGGNGGSIGANSGIVGGSGGAGGAGGAGG----NGSLSSGEGGKGGDGGH 295 G G+G N GA G+I +G GGA G G S GG GH Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 296 GGDGVGGNSSVTQGGSGGGGGAGGAGGSGF 325 G G GNS G G GF Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFGF 92 Score = 32.8 bits (74), Expect = 0.003 Identities = 27/84 (32%), Positives = 33/84 (39%) Query: 193 GKGGAGGNGGDGSFGATSGPASIGVTGAPGGNGGKGGAGGSNPNGSGGDGGKGGNGGAGG 252 G G G N G S G+ G + G G + +NP G G G GG+G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 253 NGGSIGANSGIVGGSGGAGGAGGA 276 G NSG G+GG A A Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAA 86 Score = 31.2 bits (70), Expect = 0.009 Identities = 22/69 (31%), Positives = 27/69 (39%) Query: 4 SPAAHGGSGGAGGNGGAGSAGNGGAGGAGGNGGAGGNGGGGDAGNAGSGGNGGKGGDGVG 63 P G GGA G S N GG+G GG G G+ G G+ G G G + Sbjct: 23 GPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNGNSGGGSGTGGNLS 82 Query: 64 PGSTGGAGG 72 + A G Sbjct: 83 AVAAPVAFG 91 Score = 30.8 bits (69), Expect = 0.012 Identities = 30/84 (35%), Positives = 36/84 (42%), Gaps = 5/84 (5%) Query: 223 GNGGKGGAGGSNPNGSGGDGGKGGNGGAGGNGGSIGANSGIVGGSGGAGGAGGAGGNGSL 282 G G GA ++ N +GG G G GGA G N+ GGSG GG G Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG---- 61 Query: 283 SSGEGGKGGDGGHGGDGVGGNSSV 306 G GG G+ G G G S+V Sbjct: 62 -HGNGGGNGNSGGGSGTGGNLSAV 84 Score = 30.8 bits (69), Expect = 0.012 Identities = 31/80 (38%), Positives = 34/80 (42%), Gaps = 7/80 (8%) Query: 83 SNGNARGGNAGNGGHGGAGGSGDTGGAGGAGGQGGFGGT-------GGSGSGIGGGAGGN 135 S G+ RG N G G G TG G G G G + GGSGSGI G G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 136 GGNGGAGGTGVVLGGKGGDG 155 GNGG G G GG+ Sbjct: 62 HGNGGGNGNSGGGSGTGGNL 81 Score = 30.8 bits (69), Expect = 0.015 Identities = 30/83 (36%), Positives = 39/83 (46%), Gaps = 1/83 (1%) Query: 290 GGDG-GHGGDGVGGNSSVTQGGSGGGGGAGGAGGSGFFGGKGGFGGDGGQGGPNGGGTVG 348 GGDG GH + ++ G +G G G G + GSG+ +GG G G GGG+ Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 349 TVAGGGGNGGVGGRGGDGVFAGA 371 GG GN G G G + A A Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVA 85 Score = 30.5 bits (68), Expect = 0.019 Identities = 32/84 (38%), Positives = 33/84 (39%), Gaps = 5/84 (5%) Query: 333 GGDGGQGGPNGGGTVGTVAGGGGNGGVGGRGGDGVFAGAGGQGGLGGQGGNGGGSTGGNG 392 GGDG T G + GG GVGG DG G G G GS G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDG-----SGWSSENNPWGGGSGSGIHWG 57 Query: 393 GLGGAGGGGGNAPDGGFGGNGGKG 416 G G G GGGN GG G GG Sbjct: 58 GGSGHGNGGGNGNSGGGSGTGGNL 81 Score = 30.1 bits (67), Expect = 0.021 Identities = 25/86 (29%), Positives = 31/86 (36%) Query: 369 AGAGGQGGLGGQGGNGGGSTGGNGGLGGAGGGGGNAPDGGFGGNGGKGGQGGIGGGTQSA 428 +G G+G G G GG GLG GG + G G GI G S Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 429 TGLGGDGGDGGDGGNGGNSGAKAGGA 454 G GG G+ G G G + + Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAAP 87 Score = 30.1 bits (67), Expect = 0.023 Identities = 33/109 (30%), Positives = 40/109 (36%), Gaps = 9/109 (8%) Query: 108 GAGGAGGQGGFGGTGGSGSGIGGGAGGNGGNGGAGGTGVVLGGKGGDGGNGDHGGPATNP 167 G G G G T G+ +G G G GG G GG G+G H G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGG---- 58 Query: 168 GSGSRGGAGGSGGNGGAGGNATGSGGKGGAGGNGGDGSFGATSGPASIG 216 G G+GG G G +G+GG A F A S P + G Sbjct: 59 -----GSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG 102
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv3345c	cloacin	40	8e-05	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 40.1 bits (93), Expect = 8e-05 Identities = 31/104 (29%), Positives = 37/104 (35%), Gaps = 2/104 (1%) Query: 1174 LGGNGGAGGNGGVSTTGGD--GGAGGKGGNGGDGGNVGLGGDAGSGGAGGNGGIGTDAGG 1231 + G G G N G +T G+ GG G G GG G + G G GI G Sbjct: 1 MSGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGS 60 Query: 1232 AGGAGGAGGNGGSSKSTTTGNAGSGGAGGNGGTGLNGAGGAGGA 1275 G GG GN G T + G L+ G G A Sbjct: 61 GHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 39.7 bits (92), Expect = 8e-05 Identities = 41/121 (33%), Positives = 50/121 (41%), Gaps = 5/121 (4%) Query: 1311 AGGKGGNGSSGAASGSGVVNVTAGHGGNGGNGGNGGNGSAGAGGQGGAGGSAGNGGHGGG 1370 +GG G ++GA S SG +N G GG +G S+ GG GS + G G G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 1371 ATGGDGGNGGNGGNSGNSTGVAGLAGGAAGAGGNGGGTSSAAGHGGSGGSGGSGTTGGAG 1430 GNGG GNSG +G G A G S G GG S +G A Sbjct: 62 -----HGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAAI 116 Query: 1431 A 1431 A Sbjct: 117 A 117 Score = 38.5 bits (89), Expect = 2e-04 Identities = 36/100 (36%), Positives = 46/100 (46%), Gaps = 2/100 (2%) Query: 962 GNGGNGGNGGKGGTAGNGSGAAGGNGGNGG-SGLNGGDAGNGGNGGGALNQAGFFGTGGK 1020 G G G N G T+GN +G G G GG S +G + N GGG+ + + G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 1021 GGNGGNGGAGMINGGLGGFGGAGGGGAVDVAA-TTGGAGG 1059 G GGNG +G +G G A A +T GAGG Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG 102 Score = 37.8 bits (87), Expect = 4e-04 Identities = 38/104 (36%), Positives = 43/104 (41%), Gaps = 3/104 (2%) Query: 562 SGGNGGNGGAGATPTVAGENGGAGGNGGHGGSVGNGGAGGAGGNGVAGTGLALNGGNGGN 621 SGG+G GA T NGG G G GG + G+G + N G G GG Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGG--ASDGSGWSSENNPWGGGSGSGIHWGGG 59 Query: 622 GGIGGNGGSAAGTGGDGGKGGNGGAGANGQDFSASANGANGGQG 665 G GNGG +GG G GGN A A F A G G Sbjct: 60 SG-HGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG 102 Score = 37.0 bits (85), Expect = 6e-04 Identities = 26/85 (30%), Positives = 34/85 (40%) Query: 599 AGGAGGNGVAGTGLALNGGNGGNGGIGGNGGSAAGTGGDGGKGGNGGAGANGQDFSASAN 658 +GG G G NGG G+G GG++ G+G GG +G + + Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 659 GANGGQGGNGGNGGIGGKGGDAFAT 683 NGG GN G G G A A Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAA 86 Score = 36.6 bits (84), Expect = 9e-04 Identities = 34/109 (31%), Positives = 44/109 (40%), Gaps = 3/109 (2%) Query: 1167 GPEGNGGLGGNGGAGGNGGVSTTGGDGGAGGKGGNGGDGGNVGLGGDAGSG---GAGGNG 1223 G +G G G GN TG G G G+G N GG +GSG G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 1224 GIGTDAGGAGGAGGAGGNGGSSKSTTTGNAGSGGAGGNGGTGLNGAGGA 1272 G G G +GG G GGN + + + G GG ++ + GA Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGA 111 Score = 36.2 bits (83), Expect = 0.001 Identities = 35/116 (30%), Positives = 40/116 (34%), Gaps = 8/116 (6%) Query: 1337 GNGGNGGNGGNGSAGAGGQGGAGGSAGNGGHGGGATGGDGGNGGNGGNSGNSTGVAGLAG 1396 G G G N G S GG G GG G +G N GG SG+ G +G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDG-SGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 1397 GAAGAGGNGGGTSSAAGHGGSGGSGGSGTTGGAGAAGGNGGAGAGGGSLSTGQSGG 1452 G G G GGSG G A G + G G L+ S G Sbjct: 62 HGNGGGNGNSG-------GGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAG 110 Score = 35.8 bits (82), Expect = 0.001 Identities = 29/80 (36%), Positives = 34/80 (42%), Gaps = 3/80 (3%) Query: 1291 GGDGGNGGNGGHGGDGTTGGAGGKGGNGSSGAASGSG--VVNVTAGHGGNGGNGGNGGNG 1348 GGDG G H G G G GA+ GSG N G G G GG+G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLG-VGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 1349 SAGAGGQGGAGGSAGNGGHG 1368 GG G +GG +G GG+ Sbjct: 62 HGNGGGNGNSGGGSGTGGNL 81 Score = 35.8 bits (82), Expect = 0.002 Identities = 24/76 (31%), Positives = 32/76 (42%) Query: 747 GNGGDGGKGGSGGNVGNGGNGGAGGNGAAGQAGTPGPTSGDSGTSGTDGGAGGNGGAGGA 806 G G + G + GN+ G G G GA+ +G + G SG+ GG G G Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNG 65 Query: 807 GGTLAGHGGNGGKGGN 822 GG GG+G G Sbjct: 66 GGNGNSGGGSGTGGNL 81 Score = 35.1 bits (80), Expect = 0.003 Identities = 28/97 (28%), Positives = 33/97 (34%), Gaps = 1/97 (1%) Query: 796 GAGGNGGAGGAGGTLAGHGGNGGKGGNGGQG-GIGGAGERGADGAGPNANGANGENGGSG 854 G G N GA G + G G GG G G G+G + G G+G Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNG 65 Query: 855 GNGGDGGAGGNGGAGGKAQAAGYTDGATGTGGDGGNG 891 G G+ G G G A AA G G G Sbjct: 66 GGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG 102 Score = 35.1 bits (80), Expect = 0.003 Identities = 27/83 (32%), Positives = 34/83 (40%) Query: 1350 AGAGGQGGAGGSAGNGGHGGGATGGDGGNGGNGGNSGNSTGVAGLAGGAAGAGGNGGGTS 1409 +G G+G G+ G+ G G G GG SG S+ GG+ GGG+ Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 1410 SAAGHGGSGGSGGSGTTGGAGAA 1432 G G GGSGT G A Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAV 84 Score = 34.7 bits (79), Expect = 0.003 Identities = 29/104 (27%), Positives = 31/104 (29%) Query: 1136 LGGNGGLGGNGGVSETGFGGAGGNGGYGGPGGPEGNGGLGGNGGAGGNGGVSTTGGDGGA 1195 + G G G N G T GG G G GG G G G S GG+ Sbjct: 1 MSGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGS 60 Query: 1196 GGKGGNGGDGGNVGLGGDAGSGGAGGNGGIGTDAGGAGGAGGAG 1239 G G G G G G A GAGG Sbjct: 61 GHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 34.7 bits (79), Expect = 0.003 Identities = 36/102 (35%), Positives = 40/102 (39%), Gaps = 3/102 (2%) Query: 1056 GAGGNGGAGGFASTGLGGPGGAGGPGGAGDFASGVGGVGGAGGDGGAGGVGGFGGQGGIG 1115 G G N GA + GGP G G GGA D SG G G G+ GG G Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGGASD-GSGWSSENNPWGGGSGSGIHWGGGSGHGN 64 Query: 1116 GEGRTGGNGGSGGDGGGGISLGGNGGLGGNGGVSETGFGGAG 1157 G G GN G G GG +S G +S G GG Sbjct: 65 GGG--NGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 34.3 bits (78), Expect = 0.004 Identities = 31/110 (28%), Positives = 37/110 (33%), Gaps = 10/110 (9%) Query: 379 GGIGATANSPLQAGGAGGNGGHGGLVGNGGTGGAGGAGHAGSTGATGTALQPTGGNGTNG 438 GG G N+ + NGG GL GG G + G+ G G+ Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 439 GAGGHGGNGGNGGAQHGDGGVGGKGGAGGSGGAGGNGFDAATLGSPGADG 488 G GG GN G G G S A F L +PGA G Sbjct: 63 GNGGGNGNSGGGSG----------TGGNLSAVAAPVAFGFPALSTPGAGG 102 Score = 34.3 bits (78), Expect = 0.004 Identities = 28/101 (27%), Positives = 34/101 (33%), Gaps = 1/101 (0%) Query: 826 GGIGGAGERGADGAGPNANGANGENGGSGGNGGDGGAGGNGGAGGKAQAAGYTDGATGTG 885 GG G GA N NG G GG G G +G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 886 GDGGNGGDGGKAGDGGAGENGLNSGAMLPGGGTVGNPGTGG 926 G+GG G+ G G G G + + G + PG GG Sbjct: 63 GNGGGNGNSG-GGSGTGGNLSAVAAPVAFGFPALSTPGAGG 102 Score = 34.3 bits (78), Expect = 0.004 Identities = 37/126 (29%), Positives = 48/126 (38%), Gaps = 10/126 (7%) Query: 1284 VSFGNAVGGDGGNGGNGGHGGDGTTGGAGGKGGNGSS---------GAASGSGVVNVTAG 1334 +S G+ G + G G+ G TG G G + S G SGSG+ Sbjct: 1 MSGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGS 60 Query: 1335 HGGNGGNGGNGGNGSAGAGGQGGAGGSAGNGGHGGGATGGDGGNGGNGGNSGNSTGVAGL 1394 GNGG GN G GS G GG A + G +T G GG + S +A + Sbjct: 61 GHGNGGGNGNSGGGS-GTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAAIADI 119 Query: 1395 AGGAAG 1400 G Sbjct: 120 MAALKG 125 Score = 33.9 bits (77), Expect = 0.005 Identities = 25/78 (32%), Positives = 31/78 (39%) Query: 1211 GGDAGSGGAGGNGGIGTDAGGAGGAGGAGGNGGSSKSTTTGNAGSGGAGGNGGTGLNGAG 1270 GGD G + G GG G G GG S ++ N GG+G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 1271 GAGGAGGNAGVAGVSFGN 1288 G GG GN+G + GN Sbjct: 63 GNGGGNGNSGGGSGTGGN 80 Score = 33.9 bits (77), Expect = 0.006 Identities = 24/86 (27%), Positives = 30/86 (34%) Query: 1347 NGSAGAGGQGGAGGSAGNGGHGGGATGGDGGNGGNGGNSGNSTGVAGLAGGAAGAGGNGG 1406 +G G G GA ++GN G G GG G S + G +G GG G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 1407 GTSSAAGHGGSGGSGGSGTTGGAGAA 1432 + GGSG G A Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAAP 87 Score = 33.5 bits (76), Expect = 0.008 Identities = 25/71 (35%), Positives = 34/71 (47%) Query: 876 GYTDGATGTGGDGGNGGDGGKAGDGGAGENGLNSGAMLPGGGTVGNPGTGGNGGNGGNAG 935 G+ GA T G+ G G G G + +G +S GGG+ GG G+G G Sbjct: 8 GHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGG 67 Query: 936 VGGTGGKAGTG 946 G +GG +GTG Sbjct: 68 NGNSGGGSGTG 78 Score = 33.1 bits (75), Expect = 0.008 Identities = 41/126 (32%), Positives = 44/126 (34%), Gaps = 9/126 (7%) Query: 1096 AGGDGGAGGVGGFGGQGGIGGEGRTGGNGGSGGDGGGGISLGGNGGLGGNGGVSETGFGG 1155 +GGDG G G I G G TG G G G G S N GG+G G G Sbjct: 2 SGGDGRGHNTGAHSTSGNING-GPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGS 60 Query: 1156 AGGNGGYGGPGGPEGNGGLGGNGGAGGNGGVSTTGGDGGAGGKGGNGGDGGNVGLGGDAG 1215 GNGG GNG GG G GGN G G G V + A Sbjct: 61 GHGNGG--------GNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGAL 112 Query: 1216 SGGAGG 1221 S Sbjct: 113 SAAIAD 118 Score = 33.1 bits (75), Expect = 0.010 Identities = 32/102 (31%), Positives = 38/102 (37%) Query: 917 GTVGNPGTGGNGGNGGNAGVGGTGGKAGTGSLTGLDGTDGITPNGGNGGNGGNGGKGGTA 976 G G G GN G TG G G+ G + P GG G+G + G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 977 GNGSGAAGGNGGNGGSGLNGGDAGNGGNGGGALNQAGFFGTG 1018 GNG G GG+G G A G AL+ G G Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 33.1 bits (75), Expect = 0.010 Identities = 35/102 (34%), Positives = 44/102 (43%), Gaps = 1/102 (0%) Query: 175 GSGGAGAAGGVGGSGGWLNGNGGAGGAGGTGANGGAGGNAWLFGAGGSGGAGTNGGVGGS 234 G G G G + G +NG G GG GA+ G+G ++ GG G+G + G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGG-GASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 235 GGFVYGNGGAGGIGGIGGIGGNGGDAGLFGNGGAGGAGAAGL 276 G GNG +GG G GG FG GA GL Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGL 103 Score = 32.0 bits (72), Expect = 0.020 Identities = 39/114 (34%), Positives = 47/114 (41%), Gaps = 4/114 (3%) Query: 285 GDGSDGGNGGTGGN--GGRGGLLVGNGGAGGAG-GVGGDGGKGGAGDPSFAVNNGAGGNG 341 G G + G T GN GG GL VG G + G+G + GG+G GNG Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNG 65 Query: 342 GHGGNPGVGGAGGAGGLLAGAHGAAGATPTSGGNGGDGGIGATANSPLQAGGAG 395 G GN G GG+G G L A A A P G G + + L A A Sbjct: 66 GGNGNSG-GGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAAIAD 118 Score = 32.0 bits (72), Expect = 0.021 Identities = 27/86 (31%), Positives = 32/86 (37%), Gaps = 7/86 (8%) Query: 901 GAGENGLNSGAMLPGGGTVGNPGTGGNGGNGGNAGVGGTGGKAGTGSLTGLDGTDGITPN 960 G G N+GA G G P G GG G + G + G G + Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGG-------GASDGSGWSSENNPWGGGSGSGIH 55 Query: 961 GGNGGNGGNGGKGGTAGNGSGAAGGN 986 G G GNGG G +G GSG G Sbjct: 56 WGGGSGHGNGGGNGNSGGGSGTGGNL 81 Score = 31.6 bits (71), Expect = 0.025 Identities = 39/121 (32%), Positives = 47/121 (38%), Gaps = 8/121 (6%) Query: 1115 GGEGRTGGNGGSGGDGGGGISLGGNGGLGGNGGVSETGFGGAGGNGGYGGPGGPEGNGGL 1174 GG+GR G+ G I+ GG GLG GG S+ + N GG G GG Sbjct: 3 GGDGR--GHNTGAHSTSGNIN-GGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGG 59 Query: 1175 GGNGGAGGNGGVSTTGGDGGAGGKGGNGGDGGNVGLGGDAGSGGAGGNGGIGTDAGGAGG 1234 G+G GGNG GG+G G V G A S G + AG Sbjct: 60 SGHGNGGGNGN-----SGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSA 114 Query: 1235 A 1235 A Sbjct: 115 A 115 Score = 31.6 bits (71), Expect = 0.028 Identities = 29/102 (28%), Positives = 35/102 (34%), Gaps = 2/102 (1%) Query: 1197 GKGGNGGDGGNVGLGGDAGSGGAGGNGGIGTDAGGAGGAGGAGGNGGSSKSTTTGNAGSG 1256 G G G + G G+ G G G G G + GGS G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 1257 GAGGNGGTGLNGAGGAGGAGGNAGVAGVSFGNAVGGDGGNGG 1298 G GG G G+G G +A A V+FG G GG Sbjct: 63 GNGGGNGNSGGGSGTGG--NLSAVAAPVAFGFPALSTPGAGG 102 Score = 31.6 bits (71), Expect = 0.032 Identities = 21/71 (29%), Positives = 27/71 (38%) Query: 442 GHGGNGGNGGAQHGDGGVGGKGGAGGSGGAGGNGFDAATLGSPGADGGMGGNGGKGGDGG 501 G G G N GA G + G G GG +G ++ +P G G GG G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 502 KAGDGGAGAAG 512 G G + G Sbjct: 63 GNGGGNGNSGG 73 Score = 31.2 bits (70), Expect = 0.033 Identities = 25/78 (32%), Positives = 32/78 (41%), Gaps = 1/78 (1%) Query: 1016 GTGGKGGNGG-NGGAGMINGGLGGFGGAGGGGAVDVAATTGGAGGNGGAGGFASTGLGGP 1074 G G+G N G + +G INGG G G GG ++ G G G G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 1075 GGAGGPGGAGDFASGVGG 1092 G GG G +G + G Sbjct: 63 GNGGGNGNSGGGSGTGGN 80 Score = 31.2 bits (70), Expect = 0.034 Identities = 26/79 (32%), Positives = 32/79 (40%) Query: 221 GSGGAGTNGGVGGSGGFVYGNGGAGGIGGIGGIGGNGGDAGLFGNGGAGGAGAAGLPGAA 280 G G G N G + G + G G+GG G GG+G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 281 GLNGGDGSDGGNGGTGGNG 299 G GG+G+ GG GTGGN Sbjct: 63 GNGGGNGNSGGGSGTGGNL 81 Score = 31.2 bits (70), Expect = 0.041 Identities = 32/104 (30%), Positives = 35/104 (33%) Query: 250 IGGIGGNGGDAGLFGNGGAGGAGAAGLPGAAGLNGGDGSDGGNGGTGGNGGRGGLLVGNG 309 + G G G + G G G GL G + G G N GG G G G Sbjct: 1 MSGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGS 60 Query: 310 GAGGAGGVGGDGGKGGAGDPSFAVNNGAGGNGGHGGNPGVGGAG 353 G G GG G GG G G AV PG GG Sbjct: 61 GHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 30.8 bits (69), Expect = 0.043 Identities = 35/111 (31%), Positives = 40/111 (36%), Gaps = 2/111 (1%) Query: 538 GGAGGVSANPALNGSAGANGTAPTSGGNGGNGGAGATPTVAGENGGAGGNGGHGGSVGNG 597 GG G A + S NG G GG + GG G+G H G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWG--GGS 60 Query: 598 GAGGAGGNGVAGTGLALNGGNGGNGGIGGNGGSAAGTGGDGGKGGNGGAGA 648 G G GGNG +G G G G A T G GG + AGA Sbjct: 61 GHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGA 111
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv3347c	cloacin	32	0.034	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 32.4 bits (73), Expect = 0.034 Identities = 24/91 (26%), Positives = 33/91 (36%), Gaps = 8/91 (8%) Query: 363 GFNTGVANVGSYNTGSFNAGNTNTGGFNPGNVNTGWLNTGNTNTGIANSGNVNTGAFISG 422 G NTG + G+ N G T G + +GW + N G + SG G G Sbjct: 8 GHNTGAHSTS----GNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHG 63 Query: 423 NFSNGVLWRGDYEGLWGLSGGSTIPAIPIGL 453 N G+ G G G + A P+ Sbjct: 64 NGGGN----GNSGGGSGTGGNLSAVAAPVAF 90
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv3349c	INTIMIN	31	0.005	Intimin signature.
>INTIMIN#Intimin signature. Length = 939 Score = 30.8 bits (69), Expect = 0.005 Identities = 19/67 (28%), Positives = 33/67 (49%), Gaps = 4/67 (5%) Query: 4 DPAAAYASAIRTPGLLPNAKLVVDHFHVTTLANDALTAVRRRVTWAFHDRRGRKIDPQWA 63 +P AA TP +P + +D+ H T ND L +++ + F ++I+PQ+ Sbjct: 369 NPGAATVGVNYTP--IPLVTMGIDYRHGTGNENDLLYSMQ--FRYQFDKPWSQQIEPQYV 424 Query: 64 NRRRLLT 70 N R L+ Sbjct: 425 NELRTLS 431
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv3350c	PYOCINKILLER	37	0.002	Pyocin S killer protein signature.
>PYOCINKILLER#Pyocin S killer protein signature. Length = 617 Score = 36.7 bits (84), Expect = 0.002 Identities = 46/199 (23%), Positives = 68/199 (34%), Gaps = 17/199 (8%) Query: 3442 ISVPSIHLGLDPAVHVGSITVNPITVRTPPVLVSYSQGAVTSTSGPTSEIWVKPSFFPGI 3501 + + + LGL P+V++ ++ TV P L + ++G T+ S +++ P P Sbjct: 328 LGMDAAKLGLPPSVNLNAVAKASGTVDLPMRLTNEARGNTTTLSVVSTDGVSVPKAVPVR 387 Query: 3502 RIAPSSGGGATSTQGAYFVGPISIPSGTVTFPGFTIPLDPIDIGLPVSLT--IP-GFTI- 3557 A +T G Y V S + P P P S T +P + Sbjct: 388 MAAY------NATTGLYEVTVPSTTAEAPPLILTWTPASPPGNQNPSSTTPVVPKPVPVY 441 Query: 3558 PGGTLIPTLPLGLALSNGIPPVDIPAIVLDRILLDLHADTTIGPINVPIAGFGGAPGFGN 3617 G TL P I + I AD+ I PI V PG Sbjct: 442 EGATLTPVKATPETYPGVITLPEDLIIGFP-------ADSGIKPIYVMFRDPRDVPGAAT 494 Query: 3618 STTLPSSGFFNTGAGGGSG 3636 P SG + A G G Sbjct: 495 GKGQPVSGNWLGAASQGEG 513
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv3357	PF04605	26	0.022	Virulence-associated protein D (VapD)
>PF04605#Virulence-associated protein D (VapD) Length = 125 Score = 25.6 bits (56), Expect = 0.022 Identities = 9/38 (23%), Positives = 16/38 (42%) Query: 47 QETVYLLRSPENARRLMEAVARDKAGHSAFTKSVDELR 84 Q + Y + P N RR++ V + + + V E Sbjct: 44 QYSGYTSKEPINERRVIRIVNKLTKKFTWLGECVKEFD 81
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv3367	cloacin	40	2e-05	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 40.5 bits (94), Expect = 2e-05 Identities = 31/84 (36%), Positives = 40/84 (47%), Gaps = 1/84 (1%) Query: 445 GNGGNGGNGGTGGSGGVGGNGGIGGDGAGGGNATSTSSIPF-DAHGGNGGAGGDAGHGGT 503 G G N G T G+ G G G GA G+ S+ + P+ G GG +GHG Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNG 65 Query: 504 GGDGGDGGHAGTGGRGGLLAGQHA 527 GG+G GG +GTGG +A A Sbjct: 66 GGNGNSGGGSGTGGNLSAVAAPVA 89 Score = 38.2 bits (88), Expect = 8e-05 Identities = 29/76 (38%), Positives = 32/76 (42%), Gaps = 2/76 (2%) Query: 154 GNGGNAGLIGNGGT--GGSGGAGAAGGAGGSGGWLYGNGGNGGIGGNAIVAGGAGGNGGA 211 G G N G G GG G G GGA GW N GG G+ I GG G+G Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNG 65 Query: 212 GGAAGLWGSGGSGGQG 227 GG G G+GG Sbjct: 66 GGNGNSGGGSGTGGNL 81 Score = 37.0 bits (85), Expect = 2e-04 Identities = 25/70 (35%), Positives = 33/70 (47%) Query: 413 GGDGGNGGTGGTGGRGGDGGSGGAGGASGWLMGNGGNGGNGGTGGSGGVGGNGGIGGDGA 472 G+ G TG G G GSG + + W G+G GG G G GGNG GG Sbjct: 17 SGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNGNSGGGSG 76 Query: 473 GGGNATSTSS 482 GGN ++ ++ Sbjct: 77 TGGNLSAVAA 86 Score = 36.6 bits (84), Expect = 2e-04 Identities = 32/91 (35%), Positives = 37/91 (40%), Gaps = 4/91 (4%) Query: 307 GAAGGDGGANGGAGGAGGQAASAGSSVGGDGGNGGAGGTGTNGHAGGAGGAGGAGGRGGW 366 GA G NGG G G ++ S N GG+G+ H GG G G GG G Sbjct: 12 GAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNGNS 71 Query: 367 LVGNGGNGGNGAAGGNGAIG----GTGGAGG 393 G+G G A A G T GAGG Sbjct: 72 GGGSGTGGNLSAVAAPVAFGFPALSTPGAGG 102 Score = 35.8 bits (82), Expect = 4e-04 Identities = 33/104 (31%), Positives = 40/104 (38%), Gaps = 4/104 (3%) Query: 309 AGGDG-GANGGAGGAGGQAASAGSSVGGDGGNGGAGGTGTNGHAGGAGGAGGAGGRGGWL 367 +GGDG G N GA G + +G GG G + + G G G GG Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 368 VGNGGNGGNGAAGGNGAIGGTGGAGGVPANQGGNSALGTQPVGG 411 GNGG GN G G+ G + G AL T GG Sbjct: 62 HGNGGGNGN---SGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG 102 Score = 35.1 bits (80), Expect = 7e-04 Identities = 36/106 (33%), Positives = 43/106 (40%), Gaps = 9/106 (8%) Query: 369 GNGGNGGNGAAGGNGAIGGTGGAGGVPANQGGNSALGTQPVGGDGGDGGNGGTGGTGGRG 428 G G N G + GN G TG G A+ G + P GG G G GG G G Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGG--GSGSGIHWGGGSGHG 63 Query: 429 GDGGSGGAGGASGWLMGNGGNGGNGGTGGSGGVGGNGGIGGDGAGG 474 GG+G +GG S G GGN + G + GAGG Sbjct: 64 NGGGNGNSGGGS-------GTGGNLSAVAAPVAFGFPALSTPGAGG 102 Score = 33.5 bits (76), Expect = 0.002 Identities = 27/77 (35%), Positives = 33/77 (42%) Query: 390 GAGGVPANQGGNSALGTQPVGGDGGDGGNGGTGGTGGRGGDGGSGGAGGASGWLMGNGGN 449 G G N G +S G G G G G + G+G + GG G+ G G+ Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 450 GGNGGTGGSGGVGGNGG 466 G GG G SGG G GG Sbjct: 63 GNGGGNGNSGGGSGTGG 79 Score = 33.1 bits (75), Expect = 0.003 Identities = 33/116 (28%), Positives = 41/116 (35%), Gaps = 12/116 (10%) Query: 443 LMGNGGNGGNGGTGGSGGVGGNGGIGGDGAGGGNATSTSSIPFDAHGGNGGAGGDAGHGG 502 + G G G N G + G G G GG + S S + GG G+G G G Sbjct: 1 MSGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGS 60 Query: 503 TGGDGGDGGHAGTGGRGGLLAGQHANSGNGGGGGTGGAGGTHGTPGSGNAGGTGTG 558 G+GG G++G G SG GG A G P G G Sbjct: 61 GHGNGGGNGNSGGG------------SGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 32.4 bits (73), Expect = 0.006 Identities = 18/64 (28%), Positives = 25/64 (39%) Query: 270 DGTPGGAGVNGGNGGAGGDANGNPANTSIANAGAGGNGAAGGDGGANGGAGGAGGQAASA 329 +G P G GV GG G ++ N + +G G +G G G G G Sbjct: 21 NGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNGNSGGGSGTGGN 80 Query: 330 GSSV 333 S+V Sbjct: 81 LSAV 84 Score = 31.6 bits (71), Expect = 0.010 Identities = 26/82 (31%), Positives = 34/82 (41%), Gaps = 7/82 (8%) Query: 285 AGGDANGNPANTSIANAGAGGNGAAGGDGGANGGAGGAGGQA-----ASAGSSVGGDGGN 339 +GGD G+ NT + NG G G G + G+G + S GG Sbjct: 2 SGGDGRGH--NTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGG 59 Query: 340 GGAGGTGTNGHAGGAGGAGGAG 361 G G G NG++GG G GG Sbjct: 60 SGHGNGGGNGNSGGGSGTGGNL 81 Score = 30.8 bits (69), Expect = 0.016 Identities = 25/91 (27%), Positives = 28/91 (30%) Query: 137 GNGGAGYNSAATPGMAGGNGGNAGLIGNGGTGGSGGAGAAGGAGGSGGWLYGNGGNGGIG 196 G N P G GG + G G G+ G GG +GNGG G Sbjct: 12 GAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNGNS 71 Query: 197 GNAIVAGGAGGNGGAGGAAGLWGSGGSGGQG 227 G GG A A G G G Sbjct: 72 GGGSGTGGNLSAVAAPVAFGFPALSTPGAGG 102 Score = 30.8 bits (69), Expect = 0.016 Identities = 26/92 (28%), Positives = 32/92 (34%), Gaps = 2/92 (2%) Query: 354 AGGAGGAGGRGGWLVGNGGNGGNGAAGGNGAIGGTGGAGGVPANQGGNSALGTQPVGGDG 413 +GG G G NGG G G G + G+G N G+ G G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGG--GASDGSGWSSENNPWGGGSGSGIHWGGG 59 Query: 414 GDGGNGGTGGTGGRGGDGGSGGAGGASGWLMG 445 GNGG G G G G + A+ G Sbjct: 60 SGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFG 91 Score = 30.5 bits (68), Expect = 0.025 Identities = 32/106 (30%), Positives = 43/106 (40%), Gaps = 16/106 (15%) Query: 337 GGNGGAGGTGTNGHAGGAGGAGGAGGRGGWLVGNGGNGGNGAAGGNGAIGGTGGAGGVPA 396 GG+G TG + +G G G GG G + G+G + N GG Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGG-----GASDGSGWSSENNPWGG--------- 48 Query: 397 NQGGNSALGTQPVGGDGGDGGNGGTGGTGGRGGDGGSGGAGGASGW 442 G S + G G GGNG +GG G GG+ + A A G+ Sbjct: 49 --GSGSGIHWGGGSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGF 92 Score = 29.7 bits (66), Expect = 0.037 Identities = 23/71 (32%), Positives = 30/71 (42%), Gaps = 7/71 (9%) Query: 523 AGQHANSGNGGGGGTGGAGGTHGTPGSG-------NAGGTGTGNADSTNGGPGSDGLGGD 575 G H+ SGN GG TG G + GSG GG+G+G G G+ G G+ Sbjct: 11 TGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNGN 70 Query: 576 AFNGSRGTDGN 586 + GS Sbjct: 71 SGGGSGTGGNL 81 Score = 29.7 bits (66), Expect = 0.041 Identities = 30/86 (34%), Positives = 31/86 (36%), Gaps = 12/86 (13%) Query: 335 GDGGNGGAGGTG--TNGHAGGAGGAGGAGGRGGWLVGNGGNGGNGAAGGNGAIGGTGGAG 392 G G N GA T NG G G GGA GW N GG G I GG+G Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGG----GSGSGIHWGGGSG 61 Query: 393 GVPANQGGNSALGTQPVGGDGGDGGN 418 GNS GG G G Sbjct: 62 HGNGGGNGNSG------GGSGTGGNL 81 Score = 29.3 bits (65), Expect = 0.044 Identities = 27/88 (30%), Positives = 32/88 (36%) Query: 490 GNGGAGGDAGHGGTGGDGGDGGHAGTGGRGGLLAGQHANSGNGGGGGTGGAGGTHGTPGS 549 G G G + G T G+ G G G ++ N GGG+G G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 550 GNAGGTGTGNADSTNGGPGSDGLGGDAF 577 GN GG G S GG S AF Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAF 90
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv3388	cloacin	39	7e-05	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 38.9 bits (90), Expect = 7e-05 Identities = 38/104 (36%), Positives = 47/104 (45%), Gaps = 14/104 (13%) Query: 583 TGSGGIGGNGGAGGTGGNAGVALSVGSTGGLGGNGGSGGLGGGGGSLFGNGGAGGVGATG 642 +G G G N GA T GN NGG GLG GGG+ G+G + G Sbjct: 2 SGGDGRGHNTGAHSTSGNI--------------NGGPTGLGVGGGASDGSGWSSENNPWG 47 Query: 643 GNGGSGIGPASVGGNGGKGGVGAAGGLAGQIGNGGSGGSGGAGG 686 G GSGI G+G GG G +GG +G GN + + A G Sbjct: 48 GGSGSGIHWGGGSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFG 91 Score = 37.0 bits (85), Expect = 3e-04 Identities = 25/75 (33%), Positives = 34/75 (45%) Query: 656 GNGGKGGVGAAGGLAGQIGNGGSGGSGGAGGNGGTGDTAGNGGNGGAGAVGGNAQLIGNG 715 G G+G A +G I G +G G G + G+G ++ N GG G + Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 716 GNGGGGGNGGTGADG 730 GNGGG GN G G+ Sbjct: 63 GNGGGNGNSGGGSGT 77 Score = 36.6 bits (84), Expect = 3e-04 Identities = 31/101 (30%), Positives = 41/101 (40%), Gaps = 2/101 (1%) Query: 332 GTGGTGGAGGAAGWLYGSGGAGGAGGAGGLNNAGGATGGTGGTGGAGGSGAWLYGNGGAA 391 G G GA +G + +GG G G GG ++ G + GG GSG G G Sbjct: 6 GRGHNTGAHSTSGNI--NGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHG 63 Query: 392 GAGGNGGNNTSAGTGGVGASGGTGGNAGLIGAGGHGGAGGA 432 GGNG + +GTGG ++ G G G A Sbjct: 64 NGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 36.6 bits (84), Expect = 4e-04 Identities = 33/87 (37%), Positives = 42/87 (48%), Gaps = 1/87 (1%) Query: 569 GNGGNGGAGGTAGFTGSGGIGGNGGAGGTGGNAGVALSVGSTGGLGGNGGSGGLGGGGGS 628 G G N GA T+G +GG G G GG +G + GG G+G G G G G+ Sbjct: 6 GRGHNTGAHSTSGNI-NGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGN 64 Query: 629 LFGNGGAGGVGATGGNGGSGIGPASVG 655 GNG +GG TGGN + P + G Sbjct: 65 GGGNGNSGGGSGTGGNLSAVAAPVAFG 91 Score = 35.1 bits (80), Expect = 0.001 Identities = 30/92 (32%), Positives = 36/92 (39%), Gaps = 10/92 (10%) Query: 423 AGGHGGAGGAGGNQTGGVGNGGAGGNGGAGGAGGQLYGNGGDGGNGGAGGANIAGGNGSD 482 +GG G G + T G NGG G G GGA + + GG G+ I G GS Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 483 GGAAGHGGAGGSARLIGAGGHGGDGGAGGNTA 514 G G G G GG G G +A Sbjct: 62 HGNGGGNGNSG----------GGSGTGGNLSA 83 Score = 35.1 bits (80), Expect = 0.001 Identities = 27/87 (31%), Positives = 32/87 (36%), Gaps = 1/87 (1%) Query: 368 TGGTGGTGGAGGSGAWLYGNGGAAGAGGNGGNNTSAGTGGVGASGGTGGNAGLIGAGGHG 427 +GG G G NGG G G GG + +G G G +G I GG Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSG-IHWGGGS 60 Query: 428 GAGGAGGNQTGGVGNGGAGGNGGAGGA 454 G G GGN G G+G G Sbjct: 61 GHGNGGGNGNSGGGSGTGGNLSAVAAP 87 Score = 33.1 bits (75), Expect = 0.004 Identities = 29/103 (28%), Positives = 39/103 (37%), Gaps = 2/103 (1%) Query: 293 NGGAGGAGGGGGYLVGDGGAGGTGGAGGKNSSGGATLTGGTGGTGGAGGAAGWLYGSGGA 352 +GG G G + GG G G G + +G + GG +G GG Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGV--GGGASDGSGWSSENNPWGGGSGSGIHWGGG 59 Query: 353 GGAGGAGGLNNAGGATGGTGGTGGAGGSGAWLYGNGGAAGAGG 395 G G GG N+GG +G G A+ + GAGG Sbjct: 60 SGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG 102 Score = 32.8 bits (74), Expect = 0.005 Identities = 25/92 (27%), Positives = 35/92 (38%), Gaps = 6/92 (6%) Query: 523 GTGGDGGNGGNGGLLSGNAGAGGHGGAGGSSTATTTTGTPPTGATGGNGGNGGAGGTAGF 582 G G + G G ++G G GG + ++ P G +G GG G Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG---- 61 Query: 583 TGSGGIGGNGGAGGTGGNAGVALSVGSTGGLG 614 G GGNG +GG G G +V + G Sbjct: 62 --HGNGGGNGNSGGGSGTGGNLSAVAAPVAFG 91 Score = 32.8 bits (74), Expect = 0.006 Identities = 31/93 (33%), Positives = 39/93 (41%), Gaps = 3/93 (3%) Query: 119 GANGTAENPDGQNGGLLFGNGGN---GFTQTTAGVAGGNGGSAGLIGNGGAGGGGGAGAA 175 GA+ T+ N +G GL G G + G++ GG+G G G G GGG G + Sbjct: 12 GAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNGNS 71 Query: 176 GGLGGNGGWLYGNGGAGGIGGAGTGTGGHGGAG 208 GG G GG L G T G GG Sbjct: 72 GGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 32.8 bits (74), Expect = 0.006 Identities = 27/95 (28%), Positives = 33/95 (34%), Gaps = 1/95 (1%) Query: 188 NGGAGGIGGAGTGTGGHGGAGGAGGRAWLWGTGGAGGAGGDG-GWLFGDGGAGGTGGNGG 246 N GA G G G GG W + GG G G +G G G GG G Sbjct: 10 NTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNG 69 Query: 247 SGFNSLTSSVGGAGGAGGHAGLFGAGGTGGTGGIG 281 + + + A A F A T G GG+ Sbjct: 70 NSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 32.8 bits (74), Expect = 0.006 Identities = 36/117 (30%), Positives = 45/117 (38%), Gaps = 1/117 (0%) Query: 243 GNGGSGFNSLTSSVGGAGGAGGHAGLFGAGGTGGTGGIGGQNTETGPAASNGGAGGAGGG 302 G G G N+ S G GG GL GG G +N G + +G G G G Sbjct: 3 GGDGRGHNTGAHSTSGNIN-GGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 303 GGYLVGDGGAGGTGGAGGKNSSGGATLTGGTGGTGGAGGAAGWLYGSGGAGGAGGAG 359 G G+G +GG G GG S+ A + G G + S GA A A Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAAIAD 118 Score = 32.4 bits (73), Expect = 0.006 Identities = 31/92 (33%), Positives = 37/92 (40%), Gaps = 5/92 (5%) Query: 389 GAAGAGGNGGNNTSAGT--GGVGASGGTGGNAGLIGAGGHGGAGGAGGNQTGGVGNGGAG 446 G G G N G ++++G GG G GG + G G G G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 447 GNGGAGGAGGQLYGNGGDGGNGGAGGANIAGG 478 GNGG G G G G GGN A A +A G Sbjct: 63 GNGGGNGNSG---GGSGTGGNLSAVAAPVAFG 91 Score = 32.4 bits (73), Expect = 0.008 Identities = 28/115 (24%), Positives = 42/115 (36%), Gaps = 5/115 (4%) Query: 315 TGGAGGKNSSGGATLTGGTGGTGGAGGAAGWLYGSGGAGGAGGAGGLNNAGGATGGTGGT 374 +GG G +++G + +G G G G G + G+G + N GG +G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGG-----GASDGSGWSSENNPWGGGSGSGIHW 56 Query: 375 GGAGGSGAWLYGNGGAAGAGGNGGNNTSAGTGGVGASGGTGGNAGLIGAGGHGGA 429 GG G G G+G G + A G + AG + GA Sbjct: 57 GGGSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGA 111 Score = 32.0 bits (72), Expect = 0.009 Identities = 28/75 (37%), Positives = 30/75 (40%), Gaps = 1/75 (1%) Query: 152 GGNGGSAGLIGNGGAGGGGGAGAAGGLGGNGGWLYGNGGAGGIGGAGTGTGGHGGAGGAG 211 G N G+ GN GG G G GG GW N GG G+G GG G G G Sbjct: 8 GHNTGAHSTSGNIN-GGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGG 66 Query: 212 GRAWLWGTGGAGGAG 226 G G G GG Sbjct: 67 GNGNSGGGSGTGGNL 81 Score = 32.0 bits (72), Expect = 0.009 Identities = 34/113 (30%), Positives = 42/113 (37%), Gaps = 2/113 (1%) Query: 413 GTGGNAGLIGAGGH--GGAGGAGGNQTGGVGNGGAGGNGGAGGAGGQLYGNGGDGGNGGA 470 G G N G G+ GG G G G+G + N GG G GG G+G Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNG 65 Query: 471 GGANIAGGNGSDGGAAGHGGAGGSARLIGAGGHGGDGGAGGNTAGRRADAIAG 523 GG +GG GG A + G G A +AG + AIA Sbjct: 66 GGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAAIAD 118 Score = 31.6 bits (71), Expect = 0.013 Identities = 30/86 (34%), Positives = 37/86 (43%), Gaps = 2/86 (2%) Query: 218 GTGGAGGAGGDGGWLFG--DGGAGGTGGNGGSGFNSLTSSVGGAGGAGGHAGLFGAGGTG 275 G G GA G + G G G G + GSG++S + GG G+G H G G G Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNG 65 Query: 276 GTGGIGGQNTETGPAASNGGAGGAGG 301 G G G + TG S A A G Sbjct: 66 GGNGNSGGGSGTGGNLSAVAAPVAFG 91 Score = 31.2 bits (70), Expect = 0.016 Identities = 29/88 (32%), Positives = 37/88 (42%), Gaps = 8/88 (9%) Query: 639 GATGGNGGSGIGPASVGGNGGKGGVGAAGGLAGQIGNGGSGGSGGAGGNGGTGDTAGNGG 698 G G +G S NGG G+G G G S GSG + N G +G+G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGG--------GASDGSGWSSENNPWGGGSGSGI 54 Query: 699 NGGAGAVGGNAQLIGNGGNGGGGGNGGT 726 + G G+ GN GN G G G G + Sbjct: 55 HWGGGSGHGNGGGNGNSGGGSGTGGNLS 82 Score = 30.8 bits (69), Expect = 0.020 Identities = 38/131 (29%), Positives = 43/131 (32%), Gaps = 31/131 (23%) Query: 441 GNGGAGGNGGAGGAGGQLYGNGGDGGNGGAGGANIAGGNGSDGGAAGHGGAGGSARLIGA 500 G G G N GA G + NGG G G GGA+ G S+ G Sbjct: 3 GGDGRGHNTGAHSTSGNI--NGGPTGLGVGGGASDGSGWSSENNPWG------------- 47 Query: 501 GGHGGDGGAGGNTAGRRADAIAGTGGDGGNGGNGGLLSGNAGAGGHGGAGGSSTATTTTG 560 GG G GG + G GG GN G G G GG + A G Sbjct: 48 GGSGSGIHWGGGSGH-------GNGGGNGNSGGG---------SGTGGNLSAVAAPVAFG 91 Query: 561 TPPTGATGGNG 571 P G G Sbjct: 92 FPALSTPGAGG 102 Score = 29.7 bits (66), Expect = 0.046 Identities = 23/78 (29%), Positives = 31/78 (39%) Query: 137 GNGGNGFTQTTAGVAGGNGGSAGLIGNGGAGGGGGAGAAGGLGGNGGWLYGNGGAGGIGG 196 G G N +T+G G G+ G G G + GG+G ++ GG+G G Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNG 65 Query: 197 AGTGTGGHGGAGGAGGRA 214 G G G G G A Sbjct: 66 GGNGNSGGGSGTGGNLSA 83

Rv3502c

Rv3514

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
Rv3502c	6	21	6.127840	Probable short-chain type
Rv3503c	12	28	9.942314	Probable ferredoxin FdxD
Rv3504	11	25	9.349016	Probable acyl-CoA dehydrogenase FadE26
Rv3505	11	26	9.486230	Probable acyl-CoA dehydrogenase FadE27
Rv3506	13	28	10.189949	Fatty-acid-CoA synthetase FadD17 (fatty-acid-CoA
Rv3507	17	32	11.772814	PE-PGRS family protein PE_PGRS53
Rv3508	14	29	10.986745	PE-PGRS family protein PE_PGRS54
Rv3509c	14	30	10.655543	Probable acetohydroxyacid synthase IlvX
Rv3510c	12	31	10.087774	Conserved protein
Rv3511	12	32	10.290023	PE-PGRS family protein PE_PGRS55
Rv3512	9	31	9.493548	PE-PGRS family protein PE_PGRS56
Rv3513c	4	25	6.507313	Probable fatty-acid-CoA ligase FadD18 (fragment)
Rv3514	4	25	6.485584	PE-PGRS family protein PE_PGRS57

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv3502c	DHBDHDRGNASE	81	8e-20	2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase signature. Length = 261 Score = 80.9 bits (199), Expect = 8e-20 Identities = 73/267 (27%), Positives = 114/267 (42%), Gaps = 28/267 (10%) Query: 14 NTTDLSGKVAVVTGAAAGLGRAEALGLARLGATVVVNDVASALDASDVVDEIGAAAADAG 73 N + GK+A +TGAA G+G A A LA GA + D + ++++ ++ Sbjct: 2 NAKGIEGKIAFITGAAQGIGEAVARTLASQGAHIAAVDYNP-----EKLEKVVSSLKAEA 56 Query: 74 AKAVAVAGDISQRATADELLAS-AVGLGGLDIVVNNAGITRDRMLFNMSDEEWDAVIAVH 132 A A D+ A DE+ A +G +DI+VN AG+ R ++ ++SDEEW+A +V+ Sbjct: 57 RHAEAFPADVRDSAAIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATFSVN 116 Query: 133 LRGHFLLTRNAAAYWRDKAKDAEGGSVFGRLVNTSSEAGLVGPVGQANYAAAKAGITALT 192 G F +R+ + Y D+ G +V S V A YA++KA T Sbjct: 117 STGVFNASRSVSKYMMDRRS--------GSIVTVGSNPAGVPRTSMAAYASSKAAAVMFT 168 Query: 193 LSAARALGRYGVCANVICP-RARTAMTADVFG---AAPDVEAGQID------PLS----P 238 L Y + N++ P T M ++ A V G ++ PL P Sbjct: 169 KCLGLELAEYNIRCNIVSPGSTETDMQWSLWADENGAEQVIKGSLETFKTGIPLKKLAKP 228 Query: 239 QHVVSLVQFLASPAAAEVNGQVFIVYG 265 + V FL S A + V G Sbjct: 229 SDIADAVLFLVSGQAGHITMHNLCVDG 255
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv3507	cloacin	39	1e-04	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 38.9 bits (90), Expect = 1e-04 Identities = 35/103 (33%), Positives = 43/103 (41%) Query: 1172 NGGNGGNGNDGAVNAGTGGSGGNGGNAGGGGANGGDGGAGGAGGAGGRGGKGIDGGFGGD 1231 +GG+G N GA + +GG G GGGA+ G G + GG G GI G G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 1232 GGNGGSNNGTGAGGNGGNGGTGGVGSVGAAGGDGGNGGTGGFA 1274 GNGG N +G G G + V G GG A Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 37.8 bits (87), Expect = 3e-04 Identities = 30/79 (37%), Positives = 35/79 (44%) Query: 1154 GKGGDGGNIAAGATGTAGNGGNGGNGNDGAVNAGTGGSGGNGGNAGGGGANGGDGGAGGA 1213 G G G N A +T NGG G G G + G+G S N GG G+ GG G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 1214 GGAGGRGGKGIDGGFGGDG 1232 G GG G G G GG+ Sbjct: 63 GNGGGNGNSGGGSGTGGNL 81 Score = 37.0 bits (85), Expect = 5e-04 Identities = 34/99 (34%), Positives = 37/99 (37%), Gaps = 1/99 (1%) Query: 837 GNAGDGGNGGNAGAGGNGGSGDFGGNTTSGAS-GSGGNGGNAGTAGSGGAGGTGGTGLSG 895 G G G N G GN G G GAS GSG + N G G+G G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 896 GNGGNGGNGGNGGDGGNGAHGTVGAQFVPATSLPTPNGG 934 GNGG GN G G G +L TP G Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAG 101 Score = 37.0 bits (85), Expect = 5e-04 Identities = 35/109 (32%), Positives = 47/109 (43%), Gaps = 1/109 (0%) Query: 518 TGGNGGNGTGGVNGADNTLNPDTPGGAGEPGGAGGAGGAGGAAGGPGGTGGTGGNGGNGG 577 +GG+G G + +N G G + G+G + GG+G GG G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 578 NGGNGGNGGNGGNGGNAGNNSTNA-PVGGEGGAGGDGGAGGAGGAANGG 625 +G GGNG +GG G GN S A PV A GAGG + + G Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAG 110 Score = 36.2 bits (83), Expect = 0.001 Identities = 28/76 (36%), Positives = 33/76 (43%) Query: 1148 GNGSAGGKGGDGGNIAAGATGTAGNGGNGGNGNDGAVNAGTGGSGGNGGNAGGGGANGGD 1207 G G G GNI G TG GG + N GG G+G + GGG +G Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNG 65 Query: 1208 GGAGGAGGAGGRGGKG 1223 GG G +GG G GG Sbjct: 66 GGNGNSGGGSGTGGNL 81 Score = 36.2 bits (83), Expect = 0.001 Identities = 32/81 (39%), Positives = 37/81 (45%), Gaps = 2/81 (2%) Query: 436 GKGGNGGIGGAAVTGGVAGDGGTGGKGGTGGAGGAGNDAGSTGNPGGKGGDGGIGGAGGA 495 G G G GA T G G TG G G + G+G S NP G G GI GG+ Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSG--WSSENNPWGGGSGSGIHWGGGS 60 Query: 496 GGAAGTGNGGHAGNTGDGGDG 516 G G GNG G +G GG+ Sbjct: 61 GHGNGGGNGNSGGGSGTGGNL 81 Score = 35.8 bits (82), Expect = 0.001 Identities = 29/81 (35%), Positives = 38/81 (46%), Gaps = 1/81 (1%) Query: 1167 TGTAGNGGNGGNGN-DGAVNAGTGGSGGNGGNAGGGGANGGDGGAGGAGGAGGRGGKGID 1225 +G G G N G + G +N G G G GG + G G + + GG G+G G G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 1226 GGFGGDGGNGGSNNGTGAGGN 1246 G GG GN G +GTG + Sbjct: 62 HGNGGGNGNSGGGSGTGGNLS 82 Score = 35.1 bits (80), Expect = 0.002 Identities = 32/96 (33%), Positives = 41/96 (42%), Gaps = 3/96 (3%) Query: 1061 GGEGGVGSILGGPGGNGGTGGNASATGTNGVANAGNGGKGGDGGQFGAGGNGGAGGSVTD 1120 G G+I GGP G G GG + +G + N GG G G G+G GG + Sbjct: 12 GAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGG---N 68 Query: 1121 GSAGSTAGNGGNGGNATNGTIAGQPAGGNGSAGGKG 1156 G++G +G GGN G PA AGG Sbjct: 69 GNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 35.1 bits (80), Expect = 0.002 Identities = 25/84 (29%), Positives = 33/84 (39%) Query: 1263 GDGGNGGTGGFAGFGGTAGNGGSGGTGGAGGDGGTGGDGGNGVIAGGGGTGGNGGASGAG 1322 G G G G G G +G G G G+G N GG G+G + G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 1323 GAGGTGGFAGNGNAGGNGGTGGAS 1346 G GG G +G G+ G + A+ Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAA 86 Score = 34.3 bits (78), Expect = 0.004 Identities = 35/105 (33%), Positives = 44/105 (41%), Gaps = 5/105 (4%) Query: 1233 GNGGSNNGTGAGGNGGNGGTGGVGSVGAAGGDGGNGGTGGFAGFGGTAGNGGSGGTGGAG 1292 G G + TGA GN G G G G+G + +GG +G+G G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 1293 GDGGTGGDGGNGVIAGGGGTGGNGGASGAGGAGGTGGFAGNGNAG 1337 G+GG G+ G G GTGGN A A A G + G G Sbjct: 63 GNGGGNGNSGG-----GSGTGGNLSAVAAPVAFGFPALSTPGAGG 102 Score = 34.3 bits (78), Expect = 0.004 Identities = 30/85 (35%), Positives = 38/85 (44%), Gaps = 4/85 (4%) Query: 1184 VNAGTGGSGGNGGNAGGGGANGGDGGAGGAGGAGGRGGKGIDGGFGGDGGNGGSNNGTGA 1243 ++ G G G ++ G NGG G G GGA G + G GGS +G Sbjct: 1 MSGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWG----GGSGSGIHW 56 Query: 1244 GGNGGNGGTGGVGSVGAAGGDGGNG 1268 GG G+G GG G+ G G GGN Sbjct: 57 GGGSGHGNGGGNGNSGGGSGTGGNL 81 Score = 33.9 bits (77), Expect = 0.004 Identities = 32/102 (31%), Positives = 36/102 (35%), Gaps = 1/102 (0%) Query: 580 GNGGNGGNGGNGGNAGNNSTNAPVGGEGGAGGDGGAGGAGGAANGGTAGSQ-GTGGVGGD 638 G G G N G +GN + G GG DG + GG +GS GG G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 639 GGAGGNGGGGKAGTGNSGNFGVDGEAGFSGGAGGNGGVGGAA 680 G GGNG G V F A G GG A Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 33.9 bits (77), Expect = 0.005 Identities = 32/103 (31%), Positives = 41/103 (39%) Query: 641 AGGNGGGGKAGTGNSGNFGVDGEAGFSGGAGGNGGVGGAAGANGGTGGSGGNGGDGGAGG 700 +GG+G G G ++ G G G G + G G ++ N GGSG GG G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 701 IGGAGGNGIPGTGTEPAGGTGAKGGDGGDGGAGGAGGNAGGAG 743 G GGNG G G+ G A G + AGG Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 33.5 bits (76), Expect = 0.006 Identities = 27/78 (34%), Positives = 33/78 (42%) Query: 206 GGTGGNGGNGALLIGGGGLGGAGGMGGTGGGTGGTGGNGGNGALLIGAGGVGGAGGIGGQ 265 GG G GA G GG G+G GG + G+G + N G+G GG G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 266 GTGAGGAAGAGGTGGNGG 283 G G G GG+G G Sbjct: 63 GNGGGNGNSGGGSGTGGN 80 Score = 33.5 bits (76), Expect = 0.006 Identities = 27/86 (31%), Positives = 36/86 (41%), Gaps = 5/86 (5%) Query: 542 GGAGEPGGAGGAGGAGGAAGGPGGTGGTGGNGGNGGNGGNGGNGGNGGNGGNAGNNSTNA 601 GG G G +G GGP G G GG + G+G + N GG +G+ Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGG-----ASDGSGWSSENNPWGGGSGSGIHWG 57 Query: 602 PVGGEGGAGGDGGAGGAGGAANGGTA 627 G G GG+G +GG G +A Sbjct: 58 GGSGHGNGGGNGNSGGGSGTGGNLSA 83 Score = 33.1 bits (75), Expect = 0.008 Identities = 32/110 (29%), Positives = 40/110 (36%) Query: 164 NGGAGGAGGSGGAAGGNGGNGGWLFGAGGTGGIGGTGAPGAMGGTGGNGGNGALLIGGGG 223 +GG G +G + NGG G G G+G GG G+G GG G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 224 LGGAGGMGGTGGGTGGTGGNGGNGALLIGAGGVGGAGGIGGQGTGAGGAA 273 G GG G +GGG+G G A + G GG A Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGA 111 Score = 32.8 bits (74), Expect = 0.011 Identities = 28/85 (32%), Positives = 35/85 (41%), Gaps = 2/85 (2%) Query: 1291 AGGDGGTGGDGGNGVIAGGGGTGGNGGASGAGGAGGTGGFAGNGNAGGNGGTGGASEDGD 1350 +GGDG G G GG G GGA G++ N G G G G Sbjct: 2 SGGDGR--GHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGG 59 Query: 1351 NGNAGSGATGGTGGNGGTGGDGGAA 1375 +G+ G G +GG GTGG+ A Sbjct: 60 SGHGNGGGNGNSGGGSGTGGNLSAV 84 Score = 32.8 bits (74), Expect = 0.012 Identities = 28/83 (33%), Positives = 31/83 (37%) Query: 1299 GDGGNGVIAGGGGTGGNGGASGAGGAGGTGGFAGNGNAGGNGGTGGASEDGDNGNAGSGA 1358 G G G G T GN G G G G+G + N GG S G + GSG Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 1359 TGGTGGNGGTGGDGGAAGLGGVA 1381 G G GG G L VA Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVA 85 Score = 32.4 bits (73), Expect = 0.013 Identities = 31/111 (27%), Positives = 46/111 (41%), Gaps = 7/111 (6%) Query: 784 GKGGQGGSGGTGGSGAPIGGGAGGTGGSGGHAGKGGAGGIGAQGTTITVPGNGGNAGDGG 843 G G+G + G + I GG G G GG G+ ++ P GG+ Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGG-------ASDGSGWSSENNPWGGGSGSGIH 55 Query: 844 NGGNAGAGGNGGSGDFGGNTTSGASGSGGNGGNAGTAGSGGAGGTGGTGLS 894 GG +G G GG+G+ GG + +G + S A + G GG +S Sbjct: 56 WGGGSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVS 106 Score = 32.4 bits (73), Expect = 0.015 Identities = 28/86 (32%), Positives = 35/86 (40%), Gaps = 6/86 (6%) Query: 1133 GGNATNGTIAGQPAGGNGSAGGKGGDGGNIAAGATGTAGNGGNGGNGNDGAVNAGTGGSG 1192 G ++T+G I G P G G G G + G G G G G+G Sbjct: 12 GAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG------HGNG 65 Query: 1193 GNGGNAGGGGANGGDGGAGGAGGAGG 1218 G GN+GGG GG+ A A A G Sbjct: 66 GGNGNSGGGSGTGGNLSAVAAPVAFG 91 Score = 32.4 bits (73), Expect = 0.016 Identities = 30/95 (31%), Positives = 36/95 (37%), Gaps = 1/95 (1%) Query: 1287 GTGGAGGDGGTGGDGGNGVIAGGGGTGGNGGASGAGGAGGTGGFAGNGNAGGNGGTGGAS 1346 G G G + G GN + G G G GGAS G G G+ G GG+ Sbjct: 3 GGDGRGHNTGAHSTSGN-INGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 1347 EDGDNGNAGSGATGGTGGNGGTGGDGGAAGLGGVA 1381 GN SG GTGGN A G ++ Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALS 96 Score = 32.0 bits (72), Expect = 0.019 Identities = 27/83 (32%), Positives = 33/83 (39%) Query: 551 GGAGGAGGAAGGPGGTGGTGGNGGNGGNGGNGGNGGNGGNGGNAGNNSTNAPVGGEGGAG 610 GG G G G G+G + N GG G+G + G G GN G N + G GG Sbjct: 22 GGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNGNSGGGSGTGGNL 81 Query: 611 GDGGAGGAGGAANGGTAGSQGTG 633 A A G T G+ G Sbjct: 82 SAVAAPVAFGFPALSTPGAGGLA 104 Score = 32.0 bits (72), Expect = 0.020 Identities = 32/96 (33%), Positives = 36/96 (37%), Gaps = 2/96 (2%) Query: 733 GGAGGNAGGAGGQGGNAGQGGAGGAGGNAVIPGDGVGKAPHGDAGGSGGDGGKGGQGGSG 792 G G+ GA GN G G G G G + GGSG GG G G Sbjct: 4 GDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHG 63 Query: 793 GTGGSGAPIGGGAGGTGGSGGHAGKGGAGGIGAQGT 828 GG+G GG GTGG+ A G A T Sbjct: 64 NGGGNGN--SGGGSGTGGNLSAVAAPVAFGFPALST 97 Score = 32.0 bits (72), Expect = 0.020 Identities = 28/79 (35%), Positives = 38/79 (48%) Query: 1223 GIDGGFGGDGGNGGSNNGTGAGGNGGNGGTGGVGSVGAAGGDGGNGGTGGFAGFGGTAGN 1282 G DG G + S N G G GG GS ++ + GG+G +GG +G+ Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 1283 GGSGGTGGAGGDGGTGGDG 1301 G GG G +GG GTGG+ Sbjct: 63 GNGGGNGNSGGGSGTGGNL 81 Score = 32.0 bits (72), Expect = 0.021 Identities = 31/106 (29%), Positives = 37/106 (34%), Gaps = 5/106 (4%) Query: 618 AGGAANGGTAGSQGTGGV--GGDGGAGGNGGGGKAGTGNSGNFGVDGEAGFSGGAGGNGG 675 +GG G G+ T G GG G G GG +S N + G SG GG Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSEN---NPWGGGSGSGIHWGG 58 Query: 676 VGGAAGANGGTGGSGGNGGDGGAGGIGGAGGNGIPGTGTEPAGGTG 721 G G GG+G G + G P T AGG Sbjct: 59 GSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 32.0 bits (72), Expect = 0.021 Identities = 30/86 (34%), Positives = 40/86 (46%), Gaps = 6/86 (6%) Query: 121 GANATTPGGNGGDGGWLFGSGGNGAPGAAGQSGGNGGSAGLWGNGGAGGAGGSGGAAGGN 180 GA++T+ NGG G G G + G + ++ GG +G G GGSG GG Sbjct: 12 GAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSG----SGIHWGGGSGHGNGGG 67 Query: 181 GGNGGWLFGAGGTGGIGGTGAPGAMG 206 GN G G+G G + AP A G Sbjct: 68 NGNSGG--GSGTGGNLSAVAAPVAFG 91 Score = 31.6 bits (71), Expect = 0.023 Identities = 29/110 (26%), Positives = 36/110 (32%) Query: 667 SGGAGGNGGVGGAAGANGGTGGSGGNGGDGGAGGIGGAGGNGIPGTGTEPAGGTGAKGGD 726 SGG G G + + GG G G GGA G P G +G G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 727 GGDGGAGGAGGNAGGAGGQGGNAGQGGAGGAGGNAVIPGDGVGKAPHGDA 776 G+GG G G G GG A G + G+ + A Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGA 111 Score = 31.2 bits (70), Expect = 0.036 Identities = 28/105 (26%), Positives = 36/105 (34%), Gaps = 6/105 (5%) Query: 677 GGAAGANGGTGGSGGNGGDGGAGGIGGAGGNGIPGTGTEPAGGTGAKGGDGGDGGAGGAG 736 G G N G + GN G G G G + G +E GG G G Sbjct: 4 GDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSE------NNPWGGGSGSGIHWG 57 Query: 737 GNAGGAGGQGGNAGQGGAGGAGGNAVIPGDGVGKAPHGDAGGSGG 781 G +G G G GG+G G + + P G+GG Sbjct: 58 GGSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG 102 Score = 31.2 bits (70), Expect = 0.037 Identities = 31/77 (40%), Positives = 36/77 (46%), Gaps = 7/77 (9%) Query: 565 GTGGTGGNGGNGG-----NGGNGGNGGNGGNGGNAGNNSTNAPVGGEGGAGGD--GGAGG 617 G G G N G NGG G G GG +G +S N P GG G+G GG+G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 618 AGGAANGGTAGSQGTGG 634 G NG + G GTGG Sbjct: 63 GNGGGNGNSGGGSGTGG 79 Score = 30.8 bits (69), Expect = 0.046 Identities = 32/102 (31%), Positives = 41/102 (40%), Gaps = 2/102 (1%) Query: 314 GTGGKGGQGGDGGTGGAGGAGPVLFGHGGAGGMGGQGGTGGMGGAGGDGTTVIAAGTGGE 373 G G+G G T G GP G GG G + GG G+ + G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 374 GGTGGAAGAGGAAGARGALTS--GGLAGGVGAGGTGGTGGTG 413 G GG +GG +G G L++ +A G A T G GG Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv3508	cloacin	38	3e-04	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 38.2 bits (88), Expect = 3e-04 Identities = 36/107 (33%), Positives = 41/107 (38%), Gaps = 4/107 (3%) Query: 1782 GGAGGLGGGGGTGGTNGNGGLGGGGGNGGAGGAGGTPTGSGTEGTGGDGGDAGAGGNGGS 1841 G G G + N NGG G G GGA G + + G G G GG+G Sbjct: 4 GDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHG 63 Query: 1842 ATGVGNGGNGGDGGNGGDGGNGAPGGFGGGA----GAGGLGGSGAGG 1884 G GG G G AP FG A GAGGL S + G Sbjct: 64 NGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAG 110 Score = 37.8 bits (87), Expect = 5e-04 Identities = 33/79 (41%), Positives = 39/79 (49%), Gaps = 3/79 (3%) Query: 923 GQGGQGGLGGASTTSINANGGAGGNGGTGGKGGAGGAGTLGV---GGSGGTGGDGGDAGS 979 G G+G GA +TS N NGG G G GG G + GGSG GG +G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 980 GGGGGFGGAAGKAGGGGNG 998 G GGG G + G +G GGN Sbjct: 63 GNGGGNGNSGGGSGTGGNL 81 Score = 37.8 bits (87), Expect = 5e-04 Identities = 33/79 (41%), Positives = 39/79 (49%), Gaps = 3/79 (3%) Query: 1124 GQGGQGGLGGASTTSINANGGAGGNGGTGGKGGAGGAGTLGV---GGSGGTGGDGGDAGS 1180 G G+G GA +TS N NGG G G GG G + GGSG GG +G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 1181 GGGGGFGGAAGKAGGGGNG 1199 G GGG G + G +G GGN Sbjct: 63 GNGGGNGNSGGGSGTGGNL 81 Score = 37.4 bits (86), Expect = 7e-04 Identities = 31/79 (39%), Positives = 40/79 (50%), Gaps = 2/79 (2%) Query: 538 GAGGAGGNTGVGGTNGS--GGQGGTGGAGGAGGAGGVGADNPTGIGGTGGTGGKGGAGGA 595 G G G NTG T+G+ GG G G GGA G ++N GG+G GG G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 596 GGQGGSSGAGGTNGSGGAG 614 G GG+ +GG +G+GG Sbjct: 63 GNGGGNGNSGGGSGTGGNL 81 Score = 36.6 bits (84), Expect = 9e-04 Identities = 38/119 (31%), Positives = 46/119 (38%), Gaps = 5/119 (4%) Query: 1094 GAAGNGGNGGAGGAGGAGDNNFNGGQGGAGGQGGQGGLGGASTTSINANGGAGGNGGTGG 1153 G G G N GA G N NGG G G GG G S+ + GG+G GG Sbjct: 3 GGDGRGHNTGAHSTSG----NINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGG 58 Query: 1154 KGGAGGAGTLGVGGSGGTGGDGGDAGSGGGGGFGGAAGKAGGGGNGGVGGDGGEGASGL 1212 G G G G G GG+G G + FG A G G V G ++ + Sbjct: 59 GSGHGNGGGNGNSG-GGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAAI 116 Score = 35.1 bits (80), Expect = 0.003 Identities = 36/104 (34%), Positives = 41/104 (39%), Gaps = 5/104 (4%) Query: 893 GAAGNGGNGGAGGAGGAGDNNFNGGQGGAGGQGGQGGLGGASTTSINANGGAGGNGGTGG 952 G G G N GA G N NGG G G GG G S+ + GG+G GG Sbjct: 3 GGDGRGHNTGAHSTSG----NINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGG 58 Query: 953 KGGAGGAGTLGVGGSGGTGGDGGDAGSGGGGGFGGAAGKAGGGG 996 G G G G G GG+G G + FG A G G Sbjct: 59 GSGHGNGGGNGNSG-GGSGTGGNLSAVAAPVAFGFPALSTPGAG 101 Score = 34.7 bits (79), Expect = 0.004 Identities = 32/108 (29%), Positives = 40/108 (37%), Gaps = 1/108 (0%) Query: 709 GAGGEGGAGGNSGVGGTNGSGGAGGAGGKGGTGGAGGSGADNPTGAGFAGGAGGTGGAAG 768 G G G G G G G G G + G+G S +NP G G G GG+ Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 769 AGGAGGATGTGGTGGVVGATGSAGIGGAGGRGGDGGDGASGLGLGLSG 816 G G GG+G G + A G GA GL + +S Sbjct: 63 GNGGGNGNSGGGSGT-GGNLSAVAAPVAFGFPALSTPGAGGLAVSISA 109 Score = 34.7 bits (79), Expect = 0.004 Identities = 30/83 (36%), Positives = 35/83 (42%) Query: 1410 GGAGGRGGDGGAGDSGALSQDGNGFAGGQGGQGGVGGNAGAGGINGAGGTGGTGGAGGDG 1469 GG G G GGA D S + N + GG G GG +G G G G +GG G GG+ Sbjct: 22 GGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNGNSGGGSGTGGNL 81 Query: 1470 QNGTTGVASEGGAGGQGGDGGQG 1492 VA A G GG Sbjct: 82 SAVAAPVAFGFPALSTPGAGGLA 104 Score = 34.7 bits (79), Expect = 0.004 Identities = 30/83 (36%), Positives = 35/83 (42%) Query: 1615 GGAGGRGGDGGAGDSGALSQDGNGFAGGQGGQGGVGGNAGAGGINGAGGTGGTGGAGGDG 1674 GG G G GGA D S + N + GG G GG +G G G G +GG G GG+ Sbjct: 22 GGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNGNSGGGSGTGGNL 81 Query: 1675 QNGTTGVASEGGAGGQGGDGGQG 1697 VA A G GG Sbjct: 82 SAVAAPVAFGFPALSTPGAGGLA 104 Score = 34.7 bits (79), Expect = 0.004 Identities = 31/97 (31%), Positives = 37/97 (38%) Query: 126 GGAGGAGGLLFGNGGAGAAGAPGQAGGPGGPAGLWGNGGPGGAGGSGGGTGGAGGAGGWL 185 GG G G + G+G + GG G WG G G GG G +GG G GG L Sbjct: 22 GGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNGNSGGGSGTGGNL 81 Query: 186 FGVGGAGGVGGAGGGTGGAGGPGGLIWGGGGAGGVGG 222 V G T GAGG I G + + Sbjct: 82 SAVAAPVAFGFPALSTPGAGGLAVSISAGALSAAIAD 118 Score = 33.9 bits (77), Expect = 0.006 Identities = 34/104 (32%), Positives = 38/104 (36%), Gaps = 1/104 (0%) Query: 1171 TGGDGGDAGSGGGGGFGGAAGKAGGGGNGGVGGDGGEGASGLGLGLSGFDGGQGGQGGAG 1230 +GGDG +G G G G G GG G G G S G G GG Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGG-GASDGSGWSSENNPWGGGSGSGIHWGGGS 60 Query: 1231 GSAGAGGINGAGGAGGTGGAGGDGAPATLIGGPDGGDGGQGGIG 1274 G GG +GG GTGG A G P G GG+ Sbjct: 61 GHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 33.9 bits (77), Expect = 0.006 Identities = 35/102 (34%), Positives = 39/102 (38%) Query: 759 GAGGTGGAAGAGGAGGATGTGGTGGVVGATGSAGIGGAGGRGGDGGDGASGLGLGLSGFD 818 G G G GA G G TG VG S G G + GG SG+ G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 819 GGQGGQGGAGGSAGAGGINGAGGAGGNGGDGGDGATGAAGLG 860 G GG G +GG +G GG A A G GA GL Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 33.5 bits (76), Expect = 0.008 Identities = 33/101 (32%), Positives = 39/101 (38%) Query: 1435 AGGQGGQGGVGGNAGAGGINGAGGTGGTGGAGGDGQNGTTGVASEGGAGGQGGDGGQGGI 1494 +GG G G ++ +G ING G GG DG ++ GG G G G G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 1495 GGAGGNAGFGAGVPGDGGIGGTGGAGGAGGAGADGDPSIDG 1535 G GG G G G GG A A G A P G Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG 102 Score = 33.5 bits (76), Expect = 0.008 Identities = 33/101 (32%), Positives = 39/101 (38%) Query: 1640 AGGQGGQGGVGGNAGAGGINGAGGTGGTGGAGGDGQNGTTGVASEGGAGGQGGDGGQGGI 1699 +GG G G ++ +G ING G GG DG ++ GG G G G G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 1700 GGAGGNAGFGAGVPGDGGIGGTGGAGGAGGAGADGDPSIDG 1740 G GG G G G GG A A G A P G Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG 102 Score = 33.5 bits (76), Expect = 0.009 Identities = 35/113 (30%), Positives = 50/113 (44%), Gaps = 2/113 (1%) Query: 594 GAGGQGGSSGAGGTNGS--GGAGGTGGQGGAGGAGGAGADNPTGIGGAGGTGGTGGAAGA 651 G G+G ++GA T+G+ GG G G GGA G ++N GG+G GG +G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 652 GGAGGAIGTGGTGGAVGSVGNAGIGGTGGTGGVGGAGGAGAAAAAGSSATGGA 704 G GG +GG G G++ G + G G A + + A A Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAA 115 Score = 33.1 bits (75), Expect = 0.012 Identities = 25/85 (29%), Positives = 31/85 (36%) Query: 1730 AGADGDPSIDGGQGGAGGHGGQGGKGGLNSTGLASAASGDGGNGGAGGAGGNGGAGGLGG 1789 +G DG G +G G G+ + N GG+G GG G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 1790 GGGTGGTNGNGGLGGGGGNGGAGGA 1814 G GG +GG G GGN A A Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAA 86 Score = 33.1 bits (75), Expect = 0.012 Identities = 29/88 (32%), Positives = 34/88 (38%), Gaps = 4/88 (4%) Query: 656 GAIGTGGTGGAVGSVGNAGIGGTGGTGGVGGAGGAGAAAAAGSSATGGAGFAGGAGGEGG 715 G G G GA + GN GG G+G GGA + S G +G GG Sbjct: 3 GGDGRGHNTGAHSTSGNI----NGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGG 58 Query: 716 AGGNSGVGGTNGSGGAGGAGGKGGTGGA 743 G+ GG SGG G GG A Sbjct: 59 GSGHGNGGGNGNSGGGSGTGGNLSAVAA 86 Score = 33.1 bits (75), Expect = 0.013 Identities = 32/82 (39%), Positives = 38/82 (46%), Gaps = 2/82 (2%) Query: 1821 SGTEGTGGDGGDAGAGGN-GGSATGVGNGGNGGDGGNGGDGGNGAPGGFGGGAGAGGLGG 1879 SG +G G + G GN G TG+G GG DG N GG G G GG G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 1880 SGAGGGTDGDDGNGGSPGTDGS 1901 G GGG +G+ G G G + S Sbjct: 62 HGNGGG-NGNSGGGSGTGGNLS 82 Score = 32.8 bits (74), Expect = 0.015 Identities = 27/80 (33%), Positives = 32/80 (40%) Query: 1807 GNGGAGGAGGTPTGSGTEGTGGDGGDAGAGGNGGSATGVGNGGNGGDGGNGGDGGNGAPG 1866 G G G G + SG G G G G + GS N GG G+G G G+ Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 1867 GFGGGAGAGGLGGSGAGGGT 1886 G GGG G G G G + Sbjct: 63 GNGGGNGNSGGGSGTGGNLS 82 Score = 32.8 bits (74), Expect = 0.016 Identities = 37/106 (34%), Positives = 43/106 (40%), Gaps = 4/106 (3%) Query: 569 AGGVGADNPTGIGGTGGTGGKGGAGGAGGQGGSSGAG--GTNGSGGAGGTGGQGGAGGAG 626 +GG G + TG T G G G G G S G+G N G G G GG+G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 627 GAGADNPTGIGGAGGTGGTGGAAGAGGAGG--AIGTGGTGGAVGSV 670 GG GTGG A A A G A+ T G GG S+ Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSI 107 Score = 32.4 bits (73), Expect = 0.018 Identities = 25/86 (29%), Positives = 33/86 (38%) Query: 1738 IDGGQGGAGGHGGQGGKGGLNSTGLASAASGDGGNGGAGGAGGNGGAGGLGGGGGTGGTN 1797 + GG G G G +N G +G + N GG G G GG + Sbjct: 1 MSGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGS 60 Query: 1798 GNGGLGGGGGNGGAGGAGGTPTGSGT 1823 G+G GG G +GG G GG + Sbjct: 61 GHGNGGGNGNSGGGSGTGGNLSAVAA 86 Score = 32.0 bits (72), Expect = 0.024 Identities = 36/117 (30%), Positives = 46/117 (39%), Gaps = 2/117 (1%) Query: 556 GQGGTGGAGGAGGAGGVGADNPTGIGGTGG-TGGKGGAGGAGGQGGSSGAGGTNGSGGAG 614 G G G GA G PTG+G GG + G G + GG SG+G G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 615 GTGGQGGAGGAGGAGADNPTGIGGAGGTGGTGGAA-GAGGAGGAIGTGGTGGAVGSV 670 G GG G G G N + + G + GAGG +I G A+ + Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAAIADI 119 Score = 32.0 bits (72), Expect = 0.025 Identities = 36/116 (31%), Positives = 46/116 (39%), Gaps = 3/116 (2%) Query: 1416 GGDGGAGDSGALSQDGNGFAGGQGGQGGVGGNAGAGGINGAGGTGGTGGAGGDGQNGTTG 1475 GGDG ++GA S GN G G G G + G+G + GG G+G G+ Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 1476 VASEGGAGGQGGDGGQGGIGGAGGNAGFG---AGVPGDGGIGGTGGAGGAGGAGAD 1528 G GG G G + FG PG GG+ + AG A AD Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAAIAD 118 Score = 32.0 bits (72), Expect = 0.025 Identities = 36/116 (31%), Positives = 46/116 (39%), Gaps = 3/116 (2%) Query: 1621 GGDGGAGDSGALSQDGNGFAGGQGGQGGVGGNAGAGGINGAGGTGGTGGAGGDGQNGTTG 1680 GGDG ++GA S GN G G G G + G+G + GG G+G G+ Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 1681 VASEGGAGGQGGDGGQGGIGGAGGNAGFG---AGVPGDGGIGGTGGAGGAGGAGAD 1733 G GG G G + FG PG GG+ + AG A AD Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAAIAD 118 Score = 32.0 bits (72), Expect = 0.029 Identities = 36/107 (33%), Positives = 45/107 (42%), Gaps = 4/107 (3%) Query: 970 TGGDGGDAGSGGGGGFGGAAGKAGGGGNGGRGGDGGDGASGLGLGLSGFDGGQGGQGGAG 1029 +GGDG +G G G G G GG DG SG + + GG G G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDG----SGWSSENNPWGGGSGSGIHWG 57 Query: 1030 GSAGAGGINGAGGAGGNGGDGGDGATGAAGLGDNGGVGGDGGAGGAA 1076 G +G G G G +GG G GG+ + AA + GAGG A Sbjct: 58 GGSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 32.0 bits (72), Expect = 0.030 Identities = 29/87 (33%), Positives = 34/87 (39%), Gaps = 3/87 (3%) Query: 483 GAAGTGGTGGVVGAAGKAGIGGTGGQGGAGGAGSAGTDATATGATGGTGFSGGAGGAGGA 542 G G G G +G G TG G G + +G + GG+G GG G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 543 GGNTGVGGTNGSGGQGGTGGAGGAGGA 569 G GG SGG GTGG A A Sbjct: 63 GNG---GGNGNSGGGSGTGGNLSAVAA 86 Score = 31.6 bits (71), Expect = 0.039 Identities = 42/117 (35%), Positives = 50/117 (42%), Gaps = 5/117 (4%) Query: 873 GAAGNGGNAGVGLTAKAGDGGAAGNGGNGGAG-GAGGAGDNNFNGGQGGAGGQGGQGGLG 931 G G G N G T+ +GG G G GGA G+G + +NN GG G+G G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 932 GASTTSINANGGAGGNGGTGGKGGAGGAGTLGVGGSGGTGGDGGDAGSGGGGGFGGA 988 G NG +GG GTGG A A + T G GG A S G A Sbjct: 63 GNG----GGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAA 115 Score = 31.6 bits (71), Expect = 0.039 Identities = 42/117 (35%), Positives = 50/117 (42%), Gaps = 5/117 (4%) Query: 1074 GAAGNGGNAGVGLTAKAGDGGAAGNGGNGGAG-GAGGAGDNNFNGGQGGAGGQGGQGGLG 1132 G G G N G T+ +GG G G GGA G+G + +NN GG G+G G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 1133 GASTTSINANGGAGGNGGTGGKGGAGGAGTLGVGGSGGTGGDGGDAGSGGGGGFGGA 1189 G NG +GG GTGG A A + T G GG A S G A Sbjct: 63 GNG----GGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAA 115
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv3511	cloacin	40	2e-05	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 40.1 bits (93), Expect = 2e-05 Identities = 31/89 (34%), Positives = 33/89 (37%) Query: 270 GAGGTGGNAAWLLGGGGTGGAGGIGGGNGGHGGNGGWLLGNGGNGGLGGDGDGGTGGGHG 329 G G G N G G G GG GW N GG G G GG Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 330 GNGGNPGWLLGTAGGGGNGGAGSTGTAGG 358 GNGG G G +G GGN A + A G Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFG 91 Score = 37.0 bits (85), Expect = 2e-04 Identities = 31/79 (39%), Positives = 36/79 (45%), Gaps = 1/79 (1%) Query: 237 GAGGIGGGSGGAGGNGGWLLGNGGIGGAGGTGGGAGGTGGNAAWLLGGG-GTGGAGGIGG 295 G G G +G +G G G+G GG G+G + N W G G G GG G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 296 GNGGHGGNGGWLLGNGGNG 314 GNGG GN G G GGN Sbjct: 63 GNGGGNGNSGGGSGTGGNL 81 Score = 37.0 bits (85), Expect = 3e-04 Identities = 35/115 (30%), Positives = 46/115 (40%), Gaps = 2/115 (1%) Query: 583 AGAGGTGFTQGADGNAGNGGDGGVGGNGGNGADNTTTAAAGTTGGAGGAGGAGGTGGAAG 642 +G G G GA +GN G G G GA + + ++ GG+G GG +G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 643 TGTGGQQGNGGNGGNGGTGGKGGTGGAGMNSLDPLLAAQDGGQGGTGGTGGNAGA 697 G GG GN G G GTGG A + P L+ G + G A Sbjct: 62 HGNGGGNGNSGGG--SGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSA 114 Score = 36.6 bits (84), Expect = 3e-04 Identities = 28/73 (38%), Positives = 33/73 (45%), Gaps = 5/73 (6%) Query: 233 GGHGGAGGIGGGSGGAGGNGGWLLGNGGIGGAGGTGGGAGGTGGNAAWLLGGGGTGGAGG 292 G H +G I GG G G GG + G G + GG+G W GGG G GG Sbjct: 12 GAHSTSGNINGGPTGLGVGGG---ASDGSGWSSENNPWGGGSGSGIHW--GGGSGHGNGG 66 Query: 293 IGGGNGGHGGNGG 305 G +GG G GG Sbjct: 67 GNGNSGGGSGTGG 79 Score = 36.2 bits (83), Expect = 4e-04 Identities = 28/84 (33%), Positives = 31/84 (36%), Gaps = 5/84 (5%) Query: 244 GSGGAGGNGGWLLGNGGIGGAGGTGGGAGGTGGNAAWLLGGGGTGGAGGIGGGNGGHGGN 303 G G G N G +G I G G GG + W GG G G GG G+ Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 304 GGWLLGNGGNGGLGGDGDGGTGGG 327 GNGG G G G G G Sbjct: 63 -----GNGGGNGNSGGGSGTGGNL 81 Score = 35.8 bits (82), Expect = 6e-04 Identities = 35/106 (33%), Positives = 43/106 (40%), Gaps = 6/106 (5%) Query: 378 GAGAGGHGGTGGAGGAGVNGGGAGGAGGAGGNGGAGGQAALLFGRGGTGGAGGYGGDGGG 437 GA + GG G GV GG + G+G + N GG G G GG G G G Sbjct: 12 GAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGG------GSGSGIHWGGGSGHGNG 65 Query: 438 GGDGFDGTMAGLGGTGGSGGTGGDGGAPGNGGAGGAGQLLSHSGVA 483 GG+G G +G GG + G P G G +S S A Sbjct: 66 GGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGA 111 Score = 35.5 bits (81), Expect = 7e-04 Identities = 29/100 (29%), Positives = 35/100 (35%) Query: 486 SGKGGAGGTGGNGGAGSAGADAPAGSGAMGSTGFAGGAGGDGGNGGGSGASQGNGGNGGN 545 S G G G G +D S G G+G G G G G GNG +GG Sbjct: 15 STSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNGNSGGG 74 Query: 546 GGTGGKGGTGGAGMNSLDPLLAAQDGGQGGTGGTGGNAGA 585 GTGG A + P L+ G + G A Sbjct: 75 SGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSA 114 Score = 35.5 bits (81), Expect = 8e-04 Identities = 36/104 (34%), Positives = 43/104 (41%), Gaps = 3/104 (2%) Query: 193 AGGIGGGTGGAGGHAWLFGHGGTGGIGGGPGGN--GGWLLGNGGHGGAGGIGGGSGGAGG 250 +GG G G +GG G+G G G + GW N GG G G GG G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 251 NGGWLLGNGGIGGAGGTGGGAGGTGGNAAWLLGGGGTGGAGGIG 294 +G GNG GG GTGG A+ T GAGG+ Sbjct: 62 HGNG-GGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 35.1 bits (80), Expect = 0.001 Identities = 30/81 (37%), Positives = 35/81 (43%), Gaps = 7/81 (8%) Query: 289 GAGGIGGGNGGHGGNGGWLLGNGGNGGLGGDGDGGTGGG----HGGNGGNPGWLLGTAGG 344 G G G G H +G NGG GLG G G G + GG G + GG Sbjct: 3 GGDGRGHNTGAHSTSGNI---NGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGG 59 Query: 345 GGNGGAGSTGTAGGGSGGTGG 365 G+G G G +GGGSG G Sbjct: 60 SGHGNGGGNGNSGGGSGTGGN 80 Score = 33.9 bits (77), Expect = 0.002 Identities = 35/104 (33%), Positives = 42/104 (40%), Gaps = 9/104 (8%) Query: 176 GNGGNAGWLYGRGGV-GGAGGIGGGTGGAGGHAWLFGH---GGTGGIGGGPGGNGGWLLG 231 G G N G G + GG G+G G G + G W + GG G G GG G Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG---- 61 Query: 232 NGGHGGAGGIGGGSGGAGGNGGWLLGNGGIGGAGGTGGGAGGTG 275 G+GG G GG G GGN + G + GAGG Sbjct: 62 -HGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 33.9 bits (77), Expect = 0.003 Identities = 28/95 (29%), Positives = 35/95 (36%) Query: 310 NGGNGGLGGDGDGGTGGGHGGNGGNPGWLLGTAGGGGNGGAGSTGTAGGGSGGTGGDGGT 369 N G G+ +GG G G G + G + GG+GS GGGSG G G Sbjct: 10 NTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNG 69 Query: 370 GGRGGLLMGAGAGGHGGTGGAGGAGVNGGGAGGAG 404 GG G G ++ GAGG Sbjct: 70 NSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 33.5 bits (76), Expect = 0.003 Identities = 28/85 (32%), Positives = 32/85 (37%), Gaps = 6/85 (7%) Query: 490 GAGGTGGNGGAGSAGADAPAGSGAMGSTGFAGGAGGDG------GNGGGSGASQGNGGNG 543 G G G N GA S + G +G G A G G G GSG G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 544 GNGGTGGKGGTGGAGMNSLDPLLAA 568 GNGG G G G +L + A Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAP 87 Score = 33.5 bits (76), Expect = 0.003 Identities = 33/134 (24%), Positives = 45/134 (33%), Gaps = 13/134 (9%) Query: 376 LMGAGAGGHGGTGGAGGAGVNGGGAGGAGGAGGNGGAGGQAALLFGRGGTGGAGGYGGDG 435 + G GH + +NGG G G G + G+G + GG+G +GG Sbjct: 1 MSGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGS 60 Query: 436 GGGGDGFDGTMAGLGGTGGSGGTGGDGGAPGNGGAGGAGQLLSHSGVAGASGKGGAGGTG 495 G G GG G G G G + A + G GG + Sbjct: 61 GHGN-------------GGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSI 107 Query: 496 GNGGAGSAGADAPA 509 G +A AD A Sbjct: 108 SAGALSAAIADIMA 121 Score = 32.8 bits (74), Expect = 0.005 Identities = 34/114 (29%), Positives = 39/114 (34%), Gaps = 14/114 (12%) Query: 527 GGNGGGSGASQGNGGNGGNGGTGGKGGTGGAGMNSLDPLLAAQDGGQGGTGGTGGNAGAG 586 GG+G G + NGG G G GGA G G + G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGA------------SDGSGWSSENNPWGGGS 50 Query: 587 GTGFTQGADGNAGNGGDGGVGGNGGNGADNTTTAAAGTTGG--AGGAGGAGGTG 638 G+G G GNGG G G G N + AA G A GAGG Sbjct: 51 GSGIHWGGGSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 32.0 bits (72), Expect = 0.009 Identities = 32/106 (30%), Positives = 36/106 (33%), Gaps = 10/106 (9%) Query: 421 GRGGTGGAGGYGGDGGGGGDGFDGTMAGLGGTGGSGGTGGDGGAPGNGGAGGAGQLLSHS 480 GRG GA G+ GG G G G + G G + N GG H Sbjct: 6 GRGHNTGAHSTSGNINGGPTG---------LGVGGGASDGSGWSSENNPWGGGSGSGIHW 56 Query: 481 GVAGASGKGGAGGTGGNGGAGSAGADAPAGSGAMGSTGFAG-GAGG 525 G G GG G G G A A A G + GAGG Sbjct: 57 GGGSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG 102 Score = 31.6 bits (71), Expect = 0.013 Identities = 33/101 (32%), Positives = 35/101 (34%), Gaps = 2/101 (1%) Query: 219 GGGPGGNGGWLLGNGGHGGAGGIGGGSGGAGGNGGWLLGNGGIGGAGGTGGGAGGTGG-- 276 G G G N G +G G G GGA GW N GG G+G GG G Sbjct: 4 GDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHG 63 Query: 277 NAAWLLGGGGTGGAGGIGGGNGGHGGNGGWLLGNGGNGGLG 317 N GG G GG G L G GGL Sbjct: 64 NGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 31.2 bits (70), Expect = 0.016 Identities = 27/96 (28%), Positives = 33/96 (34%), Gaps = 8/96 (8%) Query: 352 STGTAGGGSGGTGGDGGTGGRGGLLMGAGAGGHGGTGGAGGAGVNGGGAGGAGGAGGNGG 411 S G G + G G G +G G G G+G + GGG+G GG G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 412 AGGQAALLFGRGGTGGAGGYGGDGGGGGDGFDGTMA 447 G GG G G G GG +A Sbjct: 62 HGN--------GGGNGNSGGGSGTGGNLSAVAAPVA 89 Score = 30.8 bits (69), Expect = 0.021 Identities = 22/77 (28%), Positives = 29/77 (37%) Query: 572 GQGGTGGTGGNAGAGGTGFTQGADGNAGNGGDGGVGGNGGNGADNTTTAAAGTTGGAGGA 631 G+G G +G G T G + G G N G + + G G G Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNG 65 Query: 632 GGAGGTGGAAGTGTGGQ 648 GG G +GG +GTG Sbjct: 66 GGNGNSGGGSGTGGNLS 82 Score = 30.5 bits (68), Expect = 0.027 Identities = 27/101 (26%), Positives = 37/101 (36%), Gaps = 17/101 (16%) Query: 494 TGGNGGAGSAGADAPAGSGAMGSTGFAGGAGGDGGNGGGSGASQGNGGNGGNGGTGGKGG 553 +GG+G + GA + +G+ G TG G G G+G S + GG+G GG G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 554 TGGAGMNSLDPLLAAQDGGQGGTGGTGGNAGAGGTGFTQGA 594 G GG G G G + A Sbjct: 62 HGN-----------------GGGNGNSGGGSGTGGNLSAVA 85 Score = 30.5 bits (68), Expect = 0.028 Identities = 30/90 (33%), Positives = 39/90 (43%), Gaps = 13/90 (14%) Query: 345 GGNGGAGSTGTAGGGSGGTGGDGGTGGRGGLLMGAGAGG-HGGTGGAGGAGVNGGGAGGA 403 GG+G +TG GG G G GG G+G + GG G+G++ GG G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 404 GGAGGNGGAGGQAALLFGRGGTGGAGGYGG 433 G GGNG +GG G+G G Sbjct: 63 GNGGGNGNSGG------------GSGTGGN 80 Score = 30.5 bits (68), Expect = 0.029 Identities = 27/79 (34%), Positives = 29/79 (36%), Gaps = 1/79 (1%) Query: 148 GQAGGAGGSAGLLGNGGSGGAGGTGAPGGNGGNAGWLYGRGGVGGAGGIGGGTGGAGGHA 207 G G A +GG G G GG +GW GG G G GG GH Sbjct: 4 GDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHG 63 Query: 208 WLFGHGGTGGIGGGPGGNG 226 G G G G G GGN Sbjct: 64 -NGGGNGNSGGGSGTGGNL 81 Score = 29.7 bits (66), Expect = 0.048 Identities = 34/110 (30%), Positives = 38/110 (34%), Gaps = 6/110 (5%) Query: 402 GAGGAGGNGGAGGQAALLFGRGGTGGAGGYGGDGGGGGDGFDGTMAGLGGTGGSGGTGGD 461 G G G N GA + G G G GGG DG + GGSG Sbjct: 3 GGDGRGHNTGAHSTS------GNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHW 56 Query: 462 GGAPGNGGAGGAGQLLSHSGVAGASGKGGAGGTGGNGGAGSAGADAPAGS 511 GG G+G GG G SG G A G + GA A S Sbjct: 57 GGGSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVS 106 Score = 29.7 bits (66), Expect = 0.049 Identities = 27/83 (32%), Positives = 32/83 (38%), Gaps = 12/83 (14%) Query: 619 TAAAGTTGGAGGAGGAGGTGGAAGTGTGGQQGNGGNGGNGGTGGKGGTGGAGMNSLDPLL 678 T A T+G G G GG A G+G N GG G+G G G Sbjct: 11 TGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH-------- 62 Query: 679 AAQDGGQGGTGGTGGNAGAGGTG 701 G GG G +GG +G GG Sbjct: 63 ----GNGGGNGNSGGGSGTGGNL 81
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv3512	cloacin	37	5e-04	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 36.6 bits (84), Expect = 5e-04 Identities = 38/104 (36%), Positives = 44/104 (42%), Gaps = 2/104 (1%) Query: 444 GQGGAGGTGGAGAASSATNGGSGGAGGTGGDGGSGGAGGTGGAGGTGGAAGDGGQGGQGG 503 G G G GA + S NGG G G GG S G+G + GG +G G G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGG--ASDGSGWSSENNPWGGGSGSGIHWGGGS 60 Query: 504 AGGGAGGQGGAGGAGGTGGNGGNITGGTAGTAGAAGNGGAAGKG 547 G GG G +GG GTGGN + A A GA G Sbjct: 61 GHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 36.2 bits (83), Expect = 8e-04 Identities = 37/111 (33%), Positives = 47/111 (42%), Gaps = 5/111 (4%) Query: 633 GNGTGGAGGNGGGGANGGAGGAGGSGGGTGGNGGAGGDAGDAGNGGNGNGTGNGGNGGNG 692 G G + G NGG G G GG + G+G + + G G+G G G GNG Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNG 65 Query: 693 GIAGMGGNGGAGTGSGNGGNGGSGGNGGNAGMGGNSGTGSGDGGAGGNGGA 743 GGNG +G GSG GGN + G S G+G + GA Sbjct: 66 -----GGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGA 111 Score = 36.2 bits (83), Expect = 8e-04 Identities = 26/73 (35%), Positives = 31/73 (42%) Query: 588 GSGGQGGNAGGGGAGGQGGADGGSGGDGGDAGTGGNGGNGGNRNSGNGTGGAGGNGGGGA 647 G + G GG G G G G + N GG SG GG G+G GG Sbjct: 8 GHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGG 67 Query: 648 NGGAGGAGGSGGG 660 NG +GG G+GG Sbjct: 68 NGNSGGGSGTGGN 80 Score = 35.8 bits (82), Expect = 0.001 Identities = 29/78 (37%), Positives = 32/78 (41%) Query: 948 GAAGNGGNGGNAGAGGNGNGGTGGAGGIGGTGGNGGDAEPGVPPGAGGAGGAGTTGGKGG 1007 G G G N G GN NGG G G GG G + P G G G GG G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 1008 TGGNGSGTGSGGTGGDGG 1025 G G+G GG+G G Sbjct: 63 GNGGGNGNSGGGSGTGGN 80 Score = 34.7 bits (79), Expect = 0.002 Identities = 25/74 (33%), Positives = 32/74 (43%) Query: 678 GNGNGTGNGGNGGNGGIAGMGGNGGAGTGSGNGGNGGSGGNGGNAGMGGNSGTGSGDGGA 737 G+ G + NGG G+G GGA GSG G G +G+ G+G G+GG Sbjct: 8 GHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGG 67 Query: 738 GGNGGAAGTGGTGG 751 GN G G Sbjct: 68 NGNSGGGSGTGGNL 81 Score = 34.7 bits (79), Expect = 0.002 Identities = 30/78 (38%), Positives = 36/78 (46%) Query: 682 GTGNGGNGGNGGIAGMGGNGGAGTGSGNGGNGGSGGNGGNAGMGGNSGTGSGDGGAGGNG 741 G G G N G +G G G G G G + GSG + N GG SG+G GG G+G Sbjct: 4 GDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHG 63 Query: 742 GAAGTGGTGGDGGLTGTG 759 G G +GG G G Sbjct: 64 NGGGNGNSGGGSGTGGNL 81 Score = 34.7 bits (79), Expect = 0.002 Identities = 30/84 (35%), Positives = 35/84 (41%) Query: 732 SGDGGAGGNGGAAGTGGTGGDGGLTGTGGTGGSGGTGGDGGNGGNGADNTANMTAQAGGD 791 SG G G N GA T G G G G S G+G N G + + + G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 792 GGNGGDGGFGGGAGAGGGGLTAGA 815 GNGG G GG GG L+A A Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVA 85 Score = 34.3 bits (78), Expect = 0.003 Identities = 35/87 (40%), Positives = 42/87 (48%), Gaps = 3/87 (3%) Query: 658 GGGTGGNGGAGGDAGDAGNGGNGNGTGNGGNGGNGGIAGMGGNGGAGTGSGNGGNGGSGG 717 G G G N GA +G+ G G G G G + G+G + GG GSG+G + G G Sbjct: 4 GDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGG---GSGSGIHWGGGS 60 Query: 718 NGGNAGMGGNSGTGSGDGGAGGNGGAA 744 GN G GNSG GSG GG A Sbjct: 61 GHGNGGGNGNSGGGSGTGGNLSAVAAP 87 Score = 33.9 bits (77), Expect = 0.004 Identities = 31/97 (31%), Positives = 37/97 (38%) Query: 845 GNGGTGGNGGTGGTGGAGIGSLGGGTGGDGGNGGNGGTGGEGGEVGGAGGTGGAAGNGGD 904 G G G T G G LG G G G+G + GG G GG +G+G Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNG 65 Query: 905 GGTGGTGGGDGGAGGTGGTGGTGGLGDPRVGGSGGDG 941 GG G +GGG G G G P + G G Sbjct: 66 GGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG 102 Score = 33.5 bits (76), Expect = 0.005 Identities = 29/80 (36%), Positives = 32/80 (40%) Query: 420 SGGAGGAAGAGGAGGGANGTAGNGGQGGAGGTGGAGAASSATNGGSGGAGGTGGDGGSGG 479 SGG G G N G G G GG SS N GG+G GG G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 480 AGGTGGAGGTGGAAGDGGQG 499 G GG G +GG +G GG Sbjct: 62 HGNGGGNGNSGGGSGTGGNL 81 Score = 33.5 bits (76), Expect = 0.005 Identities = 24/75 (32%), Positives = 32/75 (42%) Query: 1004 GKGGTGGNGSGTGSGGTGGDGGTGGGGGNGGTGWNGGKGDTGSGGGAGDGGKAPAGGTGG 1063 G+G G S +G+ G G GGG + G+GW+ G G G+G +G G Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNG 65 Query: 1064 AGGDGGAGGKGGSGG 1078 G GG G G Sbjct: 66 GGNGNSGGGSGTGGN 80 Score = 33.1 bits (75), Expect = 0.006 Identities = 25/84 (29%), Positives = 30/84 (35%) Query: 600 GAGGQGGADGGSGGDGGDAGTGGNGGNGGNRNSGNGTGGAGGNGGGGANGGAGGAGGSGG 659 G G+G G G G G GG + G+G GGG+ G GGSG Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 660 GTGGNGGAGGDAGDAGNGGNGNGT 683 G GG G G G + Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAA 86 Score = 32.8 bits (74), Expect = 0.008 Identities = 35/109 (32%), Positives = 41/109 (37%), Gaps = 1/109 (0%) Query: 410 GNGGQGGQGGSGGAGGAAGAGGAGGGANGTAGNG-GQGGAGGTGGAGAASSATNGGSGGA 468 G G+G G+ G G G G G A +G G G G+ S GG G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 469 GGTGGDGGSGGAGGTGGAGGTGGAAGDGGQGGQGGAGGGAGGQGGAGGA 517 G GG+G SGG GTGG A G G G + GA Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGA 111 Score = 32.8 bits (74), Expect = 0.008 Identities = 35/101 (34%), Positives = 40/101 (39%), Gaps = 2/101 (1%) Query: 565 AGGDGGAGGTGGDRTVGG--GTVPAGSGGQGGNAGGGGAGGQGGADGGSGGDGGDAGTGG 622 +GGDG TG T G G G G + G G + GGSG G G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 623 NGGNGGNRNSGNGTGGAGGNGGGGANGGAGGAGGSGGGTGG 663 +G GGN NSG G+G G A G S G GG Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG 102 Score = 32.8 bits (74), Expect = 0.008 Identities = 28/86 (32%), Positives = 37/86 (43%), Gaps = 2/86 (2%) Query: 589 SGGQGGNAGGGGAGGQGGADGGSGGDGGDAGTGGNGGNGGNRNSGNGTGGAGGNGGGGAN 648 SGG G G G +GG G G G G + G+G + + GG+G G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGV--GGGASDGSGWSSENNPWGGGSGSGIHWGGG 59 Query: 649 GGAGGAGGSGGGTGGNGGAGGDAGDA 674 G G GG+G GG+G G + A Sbjct: 60 SGHGNGGGNGNSGGGSGTGGNLSAVA 85 Score = 32.4 bits (73), Expect = 0.010 Identities = 27/84 (32%), Positives = 30/84 (35%) Query: 878 GNGGTGGEGGEVGGAGGTGGAAGNGGDGGTGGTGGGDGGAGGTGGTGGTGGLGDPRVGGS 937 G G G G +G G G GG G G G G G+ G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 938 GGDGGTGGSGGAAGNGGNGGNAGA 961 G GG G SGG +G GGN A Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAA 86 Score = 32.4 bits (73), Expect = 0.012 Identities = 28/70 (40%), Positives = 29/70 (41%) Query: 844 GGNGGTGGNGGTGGTGGAGIGSLGGGTGGDGGNGGNGGTGGEGGEVGGAGGTGGAAGNGG 903 G T GN G TG G G+G N GG G G GG G G GNG Sbjct: 11 TGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNGN 70 Query: 904 DGGTGGTGGG 913 GG GTGG Sbjct: 71 SGGGSGTGGN 80 Score = 32.0 bits (72), Expect = 0.014 Identities = 32/102 (31%), Positives = 41/102 (40%) Query: 120 GGKGGNGGIGAAGTTGPVGTGASGGTGGSGGAGGTGGDGGAANGGTAGAGGAGGNGGKGG 179 GG G GA T+G + G +G G G + G+G G G GG G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 180 DGGAGVTSSTAGNSGGAGGSGGKGGDAGAGGAGATPGANGIA 221 G G +S G+ G S A A +TPGA G+A Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 32.0 bits (72), Expect = 0.015 Identities = 27/83 (32%), Positives = 32/83 (38%), Gaps = 1/83 (1%) Query: 663 GNGGAGGDAGDAGNGGNGNGTGNGGNGGNGGIAGMG-GNGGAGTGSGNGGNGGSGGNGGN 721 G G G + G GN NG G G G G G + G G+G GG G+ Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 722 AGMGGNSGTGSGDGGAGGNGGAA 744 GGN +G G G G A Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVA 85 Score = 31.6 bits (71), Expect = 0.017 Identities = 26/80 (32%), Positives = 33/80 (41%) Query: 242 GAGDGGHGGTGAAGGNGGTGGAGGSGIDGVGGGTGGTGGNGGNGAIGGAGGDAGGSGNSG 301 G G G + G + GN G G G G+G + N G G+G GG G Sbjct: 4 GDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHG 63 Query: 302 GNGGIGGKGGNAGAGGAAGS 321 GG G GG +G GG + Sbjct: 64 NGGGNGNSGGGSGTGGNLSA 83 Score = 31.6 bits (71), Expect = 0.021 Identities = 38/115 (33%), Positives = 46/115 (40%), Gaps = 3/115 (2%) Query: 700 NGGAGTGSGNGGNGGSGG-NGGNAGMGGNSGTGSGDGGAGGNGGAAGTGGTGGDGGLTGT 758 +GG G G G + SG NGG G+G G G G + N G G+G G G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWG--GG 59 Query: 759 GGTGGSGGTGGDGGNGGNGADNTANMTAQAGGDGGNGGDGGFGGGAGAGGGGLTA 813 G G GG G GG G G + +A A G G G G L+A Sbjct: 60 SGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSA 114 Score = 31.6 bits (71), Expect = 0.022 Identities = 25/84 (29%), Positives = 30/84 (35%) Query: 809 GGLTAGANGTGGQGGAGGDGGNGAIGGHGPLTDDPGGNGGTGGNGGTGGTGGAGIGSLGG 868 GG G N +GG +G G +D G + GG G+G G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 869 GTGGDGGNGGNGGTGGEGGEVGGA 892 G GG GN G G G A Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAA 86 Score = 30.8 bits (69), Expect = 0.031 Identities = 35/114 (30%), Positives = 45/114 (39%), Gaps = 1/114 (0%) Query: 19 NGGNGADNTTTAAAGTTGGAGGAGGAGGTGGTGGAAGTGTGGQQGNGGNGGNGGTGGKGG 78 +GG+G + T A + + GG G G GG +G + GG+G GG G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 79 TGGDGALAGSSGGAGGKGGNGGDAGKAGTG-SAPGTAGTGGDGGKGGNGGIGAA 131 G G S GG+G G A G A T G GG G + AA Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAA 115 Score = 30.8 bits (69), Expect = 0.034 Identities = 27/81 (33%), Positives = 32/81 (39%) Query: 898 AAGNGGDGGTGGTGGGDGGAGGTGGTGGTGGLGDPRVGGSGGDGGTGGSGGAAGNGGNGG 957 + G+G TG GG G G GG D S + GGSG GG G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 958 NAGAGGNGNGGTGGAGGIGGT 978 + GGNGN G G G + Sbjct: 62 HGNGGGNGNSGGGSGTGGNLS 82 Score = 30.8 bits (69), Expect = 0.034 Identities = 28/102 (27%), Positives = 35/102 (34%) Query: 567 GDGGAGGTGGDRTVGGGTVPAGSGGQGGNAGGGGAGGQGGADGGSGGDGGDAGTGGNGGN 626 G G G G + G +G G G+G + GG G GG G+ Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 627 GGNRNSGNGTGGAGGNGGGGANGGAGGAGGSGGGTGGNGGAG 668 G +GN GG+G G A G T G GG Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 30.8 bits (69), Expect = 0.034 Identities = 32/100 (32%), Positives = 35/100 (35%) Query: 485 GAGGTGGAAGDGGQGGQGGAGGGAGGQGGAGGAGGTGGNGGNITGGTAGTAGAAGNGGAA 544 G G G G G G G GG G + N GG +G+ G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 545 GKGGAGGQGGTGGGTGGQGGAGGDGGAGGTGGDRTVGGGT 584 G GG G G G GTGG A A G T G G Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG 102 Score = 30.5 bits (68), Expect = 0.040 Identities = 30/85 (35%), Positives = 38/85 (44%), Gaps = 2/85 (2%) Query: 305 GIGGKGGNAGAGGAAGSNGGTVGANGTGGDGGNGGAAGAATAGSNGGAGTGSAGGNGGTG 364 G G+G N GA +G+ G G G G GG +G ++ + G G+GS GG Sbjct: 3 GGDGRGHNTGAHSTSGNING--GPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGS 60 Query: 365 GRGGSGGAGGDGIGGVGGGKGGNGA 389 G G GG G G G GG A Sbjct: 61 GHGNGGGNGNSGGGSGTGGNLSAVA 85 Score = 30.5 bits (68), Expect = 0.043 Identities = 29/80 (36%), Positives = 35/80 (43%), Gaps = 2/80 (2%) Query: 801 GGGAGAGGGGLTAGANGTGGQGGAGGDGGNGAIGGHGPLTDDPGGNGGTGGNGGTGGTGG 860 G G G G + N GG G G GG A G G +++ GG+G GG G Sbjct: 4 GDGRGHNTGAHSTSGNINGGPTGLGVGGG--ASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 861 AGIGSLGGGTGGDGGNGGNG 880 G G G +GG G GGN Sbjct: 62 HGNGGGNGNSGGGSGTGGNL 81 Score = 30.5 bits (68), Expect = 0.044 Identities = 27/85 (31%), Positives = 35/85 (41%) Query: 148 SGGAGGTGGDGGAANGGTAGAGGAGGNGGKGGDGGAGVTSSTAGNSGGAGGSGGKGGDAG 207 SGG G G + G G G G G G+G +S GG+G GG +G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 208 AGGAGATPGANGIAGNGGDGGDGAA 232 G G + G +G GG+ AA Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAA 86 Score = 30.5 bits (68), Expect = 0.048 Identities = 30/102 (29%), Positives = 39/102 (38%) Query: 368 GSGGAGGDGIGGVGGGKGGNGADGEVGGAGGAGGSGPNTSPGGNGGQGGQGGSGGAGGAA 427 G G G + G G G G G + GSG ++ GG G G G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 428 GAGGAGGGANGTAGNGGQGGAGGTGGAGAASSATNGGSGGAG 469 G GG G + G +G GG A A + + G+GG Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 30.5 bits (68), Expect = 0.048 Identities = 38/109 (34%), Positives = 43/109 (39%), Gaps = 11/109 (10%) Query: 961 AGGNGNGGTGGAGGIGGTGGNGGDAEPGVPPGAGGAGGAGTTGGKGGTGGNGSGTGSGGT 1020 +GG+G G GA G G P G GG + G + N G GSG Sbjct: 2 SGGDGRGHNTGAHSTSGNINGG--------PTGLGVGGGASDGSGWSSENNPWGGGSGSG 53 Query: 1021 GGDGGTGGGGGNGGTGWNGGKGDTGSGGGAGDGGKA---PAGGTGGAGG 1066 GG G G GG G +GG TG A A PA T GAGG Sbjct: 54 IHWGGGSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG 102
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv3514	cloacin	37	7e-04	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 37.0 bits (85), Expect = 7e-04 Identities = 33/102 (32%), Positives = 41/102 (40%) Query: 479 GAAGTGGTGGMIGTTGNAGVGGAGGQGGDGGAGGAGADADQPGATGGTGFAGGAGGAGGA 538 G G G G T+GN G G G G + G+G ++ GG+G GG G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 539 GGSSGAGGTNGSGGAGGTGGQGGAGGAGGAGADNPTGIGGTG 580 G G G + G G GG A A G A + G GG Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 36.6 bits (84), Expect = 9e-04 Identities = 34/109 (31%), Positives = 40/109 (36%) Query: 505 GGDGGAGGAGADADQPGATGGTGFAGGAGGAGGAGGSSGAGGTNGSGGAGGTGGQGGAGG 564 GGDG GA + GG G GGA G S G G G GG+G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 565 AGGAGADNPTGIGGTGGDGGTGGAAGAGGAGGAAGTGGTGGMIGTTGNA 613 G G N G GTGG+ A A G + G G + + A Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGA 111 Score = 36.2 bits (83), Expect = 0.001 Identities = 29/89 (32%), Positives = 35/89 (39%) Query: 1045 GAGGKGGAGGSSGAGGTNGSGGAGGAGGQGGAGGAGISFSNGSNGGTGGTGGVGGTGGDG 1104 G G+G G+ G G G G G + G+G S N GG G+G G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 1105 GNAGTGAGDPGKGGTGGTGGTGGSGGAGG 1133 GN G G GTGG + A G Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFG 91 Score = 36.2 bits (83), Expect = 0.001 Identities = 30/90 (33%), Positives = 38/90 (42%) Query: 1244 AGGPGGKGGAGGNAGTGGTNGSGAGGAGGQGGAGGAGISFSNGSNGGTGGTGGVGGTGGD 1303 +GG G G ++ +G NG G G G + G+G S N GG G+G G G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 1304 GGNAGTGAGDPGKGGTGGTGGTGGSGGAGG 1333 GN G G GTGG + A G Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAAPVAFG 91 Score = 35.8 bits (82), Expect = 0.001 Identities = 34/109 (31%), Positives = 40/109 (36%) Query: 622 GGDGGAGGAGADADQPGATGGTGFAGGAGGAGKAGGSSSAGGTNSSGSAGGTGRQSGTGG 681 GGDG GA + GG G GGA G SS GS G G+G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 682 AGGAGADNPTGIGGTGGDGGTGGAAGAGGAGGAAGTGGTGGMIGTTGNA 730 G G N G GTGG+ A A G + G G + + A Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGA 111 Score = 35.8 bits (82), Expect = 0.001 Identities = 27/89 (30%), Positives = 34/89 (38%) Query: 1378 GKGGTNGNGGSGGTGGTGGPGGSGGAPTGSGTGGKGGAGGDGGDGADGGAATGVGDGGDG 1437 G G N G+ T G G +G G + G G + + G G+ G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 1438 GNGGNGGNGGTGVGSPGGLGGAGGTGGLG 1466 GNGG GN G G G+ G L G Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFG 91 Score = 34.7 bits (79), Expect = 0.003 Identities = 36/101 (35%), Positives = 39/101 (38%), Gaps = 3/101 (2%) Query: 1321 GTGGTGGSGGAGGSGGANFNGGTGGTGGTGGTGGKGGMGGIAG--DGGPGGDGGNAGVGG 1378 G G G + GA + G N NGG G G GG G GG G G G Sbjct: 3 GGDGRGHNTGAHSTSG-NINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 1379 KGGTNGNGGSGGTGGTGGPGGSGGAPTGSGTGGKGGAGGDG 1419 G GNG SGG GTGG + AP G G G Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG 102 Score = 34.3 bits (78), Expect = 0.004 Identities = 31/97 (31%), Positives = 37/97 (38%) Query: 126 GGAGGAGGLLFGNGGAGAAGAPGQAGGPGGPAGLWGNGGPGGAGGSGGGTGGAGGAGGWL 185 GG G G + G+G + GG G WG G G GG G +GG G GG L Sbjct: 22 GGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNGNSGGGSGTGGNL 81 Query: 186 FGVGGAGGVGGAGGGTGGAGGPGGLIWGGGGAGGVGG 222 V G T GAGG I G + + Sbjct: 82 SAVAAPVAFGFPALSTPGAGGLAVSISAGALSAAIAD 118 Score = 34.3 bits (78), Expect = 0.004 Identities = 26/79 (32%), Positives = 34/79 (43%) Query: 1218 IGGDGGQGGNGGQGDSGSGLGGQPGFAGGPGGKGGAGGNAGTGGTNGSGAGGAGGQGGAG 1277 + G G+G N G + + G P G GG G + G G+G GG Sbjct: 1 MSGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGS 60 Query: 1278 GAGISFSNGSNGGTGGTGG 1296 G G NG++GG GTGG Sbjct: 61 GHGNGGGNGNSGGGSGTGG 79 Score = 33.9 bits (77), Expect = 0.005 Identities = 32/102 (31%), Positives = 40/102 (39%), Gaps = 1/102 (0%) Query: 1070 AGGQGGAGGAGISFSNGSNGGTGGTGGVGGTGGDGGNAGTGAGDPGKGGTGGTGGTGGSG 1129 +GG G G ++G+ G GVGG DG + +P GG+G GG Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSS-ENNPWGGGSGSGIHWGGGS 60 Query: 1130 GAGGSGGANFNGGTGGTGGTGGTGGKGGMGGIAGDGGPGGDG 1171 G G GG +GG GTGG G PG G Sbjct: 61 GHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG 102 Score = 33.9 bits (77), Expect = 0.005 Identities = 32/102 (31%), Positives = 40/102 (39%), Gaps = 1/102 (0%) Query: 1270 AGGQGGAGGAGISFSNGSNGGTGGTGGVGGTGGDGGNAGTGAGDPGKGGTGGTGGTGGSG 1329 +GG G G ++G+ G GVGG DG + +P GG+G GG Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSS-ENNPWGGGSGSGIHWGGGS 60 Query: 1330 GAGGSGGANFNGGTGGTGGTGGTGGKGGMGGIAGDGGPGGDG 1371 G G GG +GG GTGG G PG G Sbjct: 61 GHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG 102 Score = 33.9 bits (77), Expect = 0.005 Identities = 32/103 (31%), Positives = 39/103 (37%), Gaps = 1/103 (0%) Query: 793 GAAGTGGTGGMIGTTGNAGVGGAGGQGGDGGAGGAGADADQPGATGGTGFAGGAGGAGGS 852 G G G G T+GN G G G G + G+G ++ GG+G GG G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 853 GGSSCAGGTNGSGGAGGTCGQVVAGGAGISFSNGSNGGTGGTG 895 G G + G G GG VA F S G GG Sbjct: 63 GNGGGNGNSGGGSGTGGN-LSAVAAPVAFGFPALSTPGAGGLA 104 Score = 33.9 bits (77), Expect = 0.005 Identities = 37/108 (34%), Positives = 42/108 (38%), Gaps = 5/108 (4%) Query: 1100 TGGDGGNAGTGAGDPGKGGTGGTGGTGGSGGAGGSGGANFNGGTGGTGGTGGTGGKGGMG 1159 +GGDG TGA GG G G GGA G + G G G GG G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 1160 GIAGDGGPGGDGGNAGVGGKGGTNGNGGSGGTGGTGGAGGNAGAGGLA 1207 G GG GN+G G G N + + A GAGGLA Sbjct: 62 -----HGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 33.5 bits (76), Expect = 0.007 Identities = 27/89 (30%), Positives = 32/89 (35%) Query: 1369 GDGGNAGVGGKGGTNGNGGSGGTGGTGGPGGSGGAPTGSGTGGKGGAGGDGGDGADGGAA 1428 G G G T+GN G TG G G S G+ S GG G G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 1429 TGVGDGGDGGNGGNGGNGGTGVGSPGGLG 1457 G G+ G G G + V +P G Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFG 91 Score = 32.8 bits (74), Expect = 0.011 Identities = 28/102 (27%), Positives = 36/102 (35%) Query: 596 GAAGTGGTGGMIGTTGNAGVGGAGGQGGDGGAGGAGADADQPGATGGTGFAGGAGGAGKA 655 G G G G T+GN G G G G + G+G ++ GG+G GG Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 656 GGSSSAGGTNSSGSAGGTGRQSGTGGAGGAGADNPTGIGGTG 697 G G + GG A G A + G GG Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 32.8 bits (74), Expect = 0.012 Identities = 36/119 (30%), Positives = 45/119 (37%), Gaps = 10/119 (8%) Query: 713 GAAGTGGTGGMIGTTGNAGVGGAGGSSGAGGTNGSGGAGGTDGQGGAGGAGGAGADNPTG 772 G G G G T+GN G G G G ++GSG + + GG G+G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSG--------- 53 Query: 773 IGGTGGDGGTGGAAGAGGAGGAAGTGGTGGMIGTTGNAGVGGAGGQGGDGGAGGAGADA 831 GG G G G G +GG +GTGG + G G G A A A Sbjct: 54 -IHWGGGSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGA 111 Score = 32.8 bits (74), Expect = 0.014 Identities = 34/101 (33%), Positives = 37/101 (36%), Gaps = 2/101 (1%) Query: 391 GGDGGAGGAGADADQPGATGGTGFAGGAGGAGGAGGSSGAGGTNGSGGAGGQGGAGGAGG 450 GGDG GA + GG G GGA G S G G G GG+G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 451 AGADNPTGIGGTGGDGGTGGAAGAGGAGG--AAGTGGTGGM 489 GG G GG A A A G A T G GG+ Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGL 103 Score = 32.4 bits (73), Expect = 0.014 Identities = 33/112 (29%), Positives = 37/112 (33%), Gaps = 9/112 (8%) Query: 1183 NGNGGSGGTGGTGGAGGNAGAGGLANTGGTAGNAGIGGDGGQGGNGGQGDSGSGLGGQPG 1242 +G G G G GN G G + G G GG SG GG G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 1243 FAGGPGGKGGAGGNAGTGGTNGSGAGGAGGQG-------GAGGAGISFSNGS 1287 G GG G G G N S G GAGG +S S G+ Sbjct: 62 --HGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGA 111 Score = 32.4 bits (73), Expect = 0.015 Identities = 40/128 (31%), Positives = 47/128 (36%), Gaps = 23/128 (17%) Query: 965 SGTGGTGGTGGKGGTGGAGDDSAGGTGGTGGAGGNAGAGGLANTGGTAGNAGIGGDGGQG 1024 SG G G G T G + G G GGA +G N G +GI GG G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 1025 GNGGQGDSGSGLGGQPGFAGGAGGKGGAGGSSGAGGTNGSGGAGGAGG-----QGGAGGA 1079 G GG G +GG SG GG + A A G GAGG Sbjct: 62 H------------------GNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGL 103 Query: 1080 GISFSNGS 1087 +S S G+ Sbjct: 104 AVSISAGA 111 Score = 32.0 bits (72), Expect = 0.023 Identities = 30/101 (29%), Positives = 39/101 (38%) Query: 363 GGAGGAAGQLFSASGAAGNAGVGGAGGQGGDGGAGGAGADADQPGATGGTGFAGGAGGAG 422 G G S SG G G G G+G + + G +G GG G G Sbjct: 4 GDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHG 63 Query: 423 GAGGSSGAGGTNGSGGAGGQGGAGGAGGAGADNPTGIGGTG 463 GG+ +GG +G+GG A A G A + G GG Sbjct: 64 NGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 31.2 bits (70), Expect = 0.040 Identities = 25/74 (33%), Positives = 31/74 (41%) Query: 877 GGAGISFSNGSNGGTGGTGGVGGTGGDGGNAGTGAGDPGKGGTGGTGGTGGSGGAGGSGG 936 G + S N G TG G G G+ + +P GG+G GG G G GG Sbjct: 8 GHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGG 67 Query: 937 ANFNGGTGGTGGTG 950 +GG GTGG Sbjct: 68 NGNSGGGSGTGGNL 81 Score = 30.8 bits (69), Expect = 0.045 Identities = 26/82 (31%), Positives = 36/82 (43%) Query: 856 SCAGGTNGSGGAGGTCGQVVAGGAGISFSNGSNGGTGGTGGVGGTGGDGGNAGTGAGDPG 915 S G + GA T G + G G+ G++ G+G + GG G+ G G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 916 KGGTGGTGGTGGSGGAGGSGGA 937 G GG G +GG G GG+ A Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSA 83

Rv3523

Rv3532

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
Rv3523	-1	14	-4.519603	Probable lipid carrier protein or keto acyl-CoA
Rv3524	-2	14	-5.226110	Probable conserved membrane protein
Rv3525c	-2	13	-5.203744	Possible siderophore-binding protein
Rv3526	-1	11	-5.055000	Oxygenase component of
Rv3527	0	13	-3.761756	Hypothetical protein
Rv3528c	3	11	-2.993440	Unknown protein
Rv3529c	2	12	-1.039153	Conserved hypothetical protein
Rv3530c	1	13	-0.386462	Possible oxidoreductase
Rv3531c	2	14	0.052834	Hypothetical protein
Rv3532	2	11	0.039892	PPE family protein PPE61

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv3530c	DHBDHDRGNASE	81	2e-20	2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase signature. Length = 261 Score = 81.3 bits (200), Expect = 2e-20 Identities = 60/254 (23%), Positives = 103/254 (40%), Gaps = 11/254 (4%) Query: 8 KVIVVSGVGPGLGTTLAHRCARDGADLVLAARSAERLDDVAKQIIDTGRRAVAVRTDITD 67 K+ ++G G+G +A A GA + + E+L+ V + R A A D+ D Sbjct: 9 KIAFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHAEAFPADVRD 68 Query: 68 DDDVSNLVQATLAAYGKADVLINNAFRVPSMKPLAGTTFEHIRDAIELSALGTLRLIQAF 127 + + G D+L+N A V + + E +++ G ++ Sbjct: 69 SAAIDEITARIEREMGPIDILVNVA-GVLRPGLIHSLSDEEWEATFSVNSTGVFNASRSV 127 Query: 128 TPAL-AQSHGAIVNVNSMVIRHSQPKYGTYKMAKSVLLAMSHSLATELGEQGIRVNSVAP 186 + + + G+IV V S + Y +K+ + + L EL E IR N V+P Sbjct: 128 SKYMMDRRSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLELAEYNIRCNIVSP 187 Query: 187 GYIWGDTLKSYFDHQAGKYGTTVDQIYQATAANSD----LKRLPTEDEVASAILFLASDL 242 G D S + + G +Q+ + + LK+L ++A A+LFL S Sbjct: 188 GSTETDMQWSLWADENGA-----EQVIKGSLETFKTGIPLKKLAKPSDIADAVLFLVSGQ 242 Query: 243 ASGITGQTLDVNCG 256 A IT L V+ G Sbjct: 243 AGHITMHNLCVDGG 256
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv3531c	PF03944	31	0.011	delta endotoxin
>PF03944#delta endotoxin Length = 633 Score = 30.8 bits (69), Expect = 0.011 Identities = 13/48 (27%), Positives = 22/48 (45%) Query: 59 SGTGPFTKMGLDNPDTLYFGTRLQANRDYVVSGRRGTTTDLSFQLLGG 106 SG+GP + D + + Q N +YV++G G +F + G Sbjct: 278 SGSGPQQTQSFTSQDWPFLYSLFQVNSNYVLNGFSGARLSNTFPNIVG 325

Rv3585

Rv3596c

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
Rv3585	0	10	4.113448	DNA repair protein RadA (DNA repair protein
Rv3586	2	11	4.304876	Conserved hypothetical protein
Rv3587c	4	10	4.938602	Probable conserved membrane protein
Rv3588c	2	10	3.850463	Beta-carbonic anhydrase CanB
Rv3589	3	9	4.047384	Probable adenine glycosylase MutY
Rv3590c	5	10	5.223410	PE-PGRS family protein PE_PGRS58
Rv3591c	0	10	1.525190	Possible hydrolase
Rv3592	0	12	1.410262	Possible heme degrading protein MhuD
Rv3593	1	10	0.238616	Probable conserved lipoprotein LpqF
Rv3594	2	11	0.468136	Conserved hypothetical protein
Rv3595c	2	10	0.015033	PE-PGRS family protein PE_PGRS59
Rv3596c	3	11	-2.460790	Probable ATP-dependent protease ATP-binding

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv3587c	PF03544	28	0.023	Gram-negative bacterial tonB protein
>PF03544#Gram-negative bacterial tonB protein Length = 243 Score = 28.4 bits (63), Expect = 0.023 Identities = 25/103 (24%), Positives = 35/103 (33%), Gaps = 4/103 (3%) Query: 27 VVVVGIAVAIVIAFVDSSAGAKPVSADKPASAQSHPGSPAPQAPQPAGQTEGNAAAAP-P 85 VV G+ V ++ A A+P+S A A P P+P + E P P Sbjct: 27 AVVAGLLYTSVHQVIELPAPAQPISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEP 86 Query: 86 QGQNP---ETPTPTAAVQPPPVLKEGDDCPDSTLAVKGLTNAP 125 + P E P P +P PV K D + Sbjct: 87 PKEAPVVIEKPKPKPKPKPKPVKKVEQPKRDVKPVESRPASPF 129
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv3590c	cloacin	39	3e-05	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 39.3 bits (91), Expect = 3e-05 Identities = 43/132 (32%), Positives = 50/132 (37%), Gaps = 2/132 (1%) Query: 449 AGTGGVGGVGGAGGQGGLLFGDGGNGGAGGAGGIGGTGASGGAGGKGGSGLVGGDGGNGG 508 +G G G GA G + G G GG G +S GGSG GG G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 509 AGGAGGNGGKGGAGGAGGGAGMFSQPGVHG--AGGTGGQGGAGGAGGAGGAAGAGTVVAG 566 G GGNG GG G GG + P G A T G GG + AG + A + Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAAIADIMA 121 Query: 567 NPGDPGGFGAAG 578 P FG G Sbjct: 122 ALKGPFKFGLWG 133 Score = 38.2 bits (88), Expect = 1e-04 Identities = 32/91 (35%), Positives = 41/91 (45%) Query: 136 GGPGGLLWGNGGNGGSGVAGVGGPGGSGGAAGLFGHGGNGGAGGSNAAGAGGVGGAGGAG 195 GGP GL G G + GSG + P G G +G+ GG+G G +GG G GG Sbjct: 22 GGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNGNSGGGSGTGGNL 81 Query: 196 WLVGNGGAGGFGGVGTTVSGNGGAGGAAGAF 226 V A GF + T +G +AGA Sbjct: 82 SAVAAPVAFGFPALSTPGAGGLAVSISAGAL 112 Score = 35.5 bits (81), Expect = 5e-04 Identities = 31/84 (36%), Positives = 40/84 (47%), Gaps = 5/84 (5%) Query: 314 AGGAGGLGGSGSDTSEGPVTGGNGGNGGDGGPGAPGGNGA---PGGIGVNTGTGWAYGGN 370 +GG G +G+ ++ G + GG G G GG G + P G G +G W GG Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHW--GGG 59 Query: 371 GGNGGDGGAGARGGDGGNGGNGLA 394 G+G GG G GG G GGN A Sbjct: 60 SGHGNGGGNGNSGGGSGTGGNLSA 83 Score = 33.5 bits (76), Expect = 0.003 Identities = 30/97 (30%), Positives = 36/97 (37%), Gaps = 10/97 (10%) Query: 395 LNGGNGIGGNGGAGGRGGTGAAGGNGGIGGGATGTLTFFGSGGDGGPGGAGANTAGTGGV 454 ++GG+G G N GA G GG G+G G G G G + N GG Sbjct: 1 MSGGDGRGHNTGAHSTSGN-INGGPTGLGV---------GGGASDGSGWSSENNPWGGGS 50 Query: 455 GGVGGAGGQGGLLFGDGGNGGAGGAGGIGGTGASGGA 491 G GG G G G GG+G G A Sbjct: 51 GSGIHWGGGSGHGNGGGNGNSGGGSGTGGNLSAVAAP 87 Score = 32.8 bits (74), Expect = 0.004 Identities = 33/97 (34%), Positives = 37/97 (38%), Gaps = 3/97 (3%) Query: 145 NGGNGGSGVAGVGGPGGSGGAAGLFGHGGNGGAGGSNAAGAGGVGGAGGAGWLVGNGGAG 204 N G + GGP G G G G G+G GG GNGG Sbjct: 10 NTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG-HGNGGGN 68 Query: 205 GFGGVGTTVSGNGGAGGAAGAFGNGGVG--GAGGAAV 239 G G G+ GN A A AFG + GAGG AV Sbjct: 69 GNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAV 105 Score = 32.8 bits (74), Expect = 0.004 Identities = 28/81 (34%), Positives = 32/81 (39%), Gaps = 3/81 (3%) Query: 383 GGDGGNGGNGLALNGGNGIGGNGGAGGRGGTGAAGGNGGIG---GGATGTLTFFGSGGDG 439 GGDG G GN GG G G GG G GG +G+ +G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 440 GPGGAGANTAGTGGVGGVGGA 460 G GG N+ G G GG A Sbjct: 63 GNGGGNGNSGGGSGTGGNLSA 83 Score = 32.8 bits (74), Expect = 0.004 Identities = 25/76 (32%), Positives = 30/76 (39%) Query: 291 GANNGAGSGGAGLPGNPGAVPGRAGGAGGLGGSGSDTSEGPVTGGNGGNGGDGGPGAPGG 350 G N GA S + G P + G + G G S + G +G GG G G GG Sbjct: 8 GHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGG 67 Query: 351 NGAPGGIGVNTGTGWA 366 NG GG G A Sbjct: 68 NGNSGGGSGTGGNLSA 83 Score = 32.4 bits (73), Expect = 0.005 Identities = 32/128 (25%), Positives = 44/128 (34%), Gaps = 10/128 (7%) Query: 335 GNGGNGGDGGPGAPGGN--GAPGGIGVNTGTGWAYGGNGGNGGDGGAGARGGDGGNGGNG 392 G G G + G + GN G P G+GV GG G G G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVG-------GGASDGSGWSSENNPWGGGSGSGIH 55 Query: 393 LALNGGNGIGGNGGAGGRGGTGAAGGNGGIGGGATGTLTFFGSGGDGGPGGAGANTAGTG 452 G+G GG G G GG+G G + + G GG + + A + Sbjct: 56 WGGGSGHGNGGGNGNSG-GGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSA 114 Query: 453 GVGGVGGA 460 + + A Sbjct: 115 AIADIMAA 122 Score = 31.6 bits (71), Expect = 0.009 Identities = 29/102 (28%), Positives = 36/102 (35%) Query: 174 NGGAGGSNAAGAGGVGGAGGAGWLVGNGGAGGFGGVGTTVSGNGGAGGAAGAFGNGGVGG 233 +GG G + GA G G G G G G + N GG+ GG G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 234 AGGAAVIGGLPGNGGAGGNAGLIGAGGDGGVGGVGAPGTNGM 275 G G G G GGN + A G + PG G+ Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGL 103 Score = 31.6 bits (71), Expect = 0.010 Identities = 31/102 (30%), Positives = 37/102 (36%), Gaps = 2/102 (1%) Query: 483 GGTGASGGAGGKGGSGLVGGDGGNGGAGGAGGNGGKGGAGGAGGGAGMFSQPGVHGAGGT 542 GG G G SG + +GG G G GG G G S G+H GG+ Sbjct: 3 GGDGRGHNTGAHSTSGNI--NGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGS 60 Query: 543 GGQGGAGGAGGAGGAAGAGTVVAGNPGDPGGFGAAGADGLPG 584 G G G GG+ G + A GF A G G Sbjct: 61 GHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG 102 Score = 30.8 bits (69), Expect = 0.016 Identities = 37/108 (34%), Positives = 41/108 (37%), Gaps = 8/108 (7%) Query: 160 GGSGGAAGLFGHGGNGGAGGSNAAGAGGVGGAGGAGWLVGNGGAGGFGGVGTTVSGN--- 216 GG G H +G G G G + G+GW N GG G G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 217 ---GGAGGAAGAFGNGGVGGAGGAAVIGGLPG--NGGAGGNAGLIGAG 259 GG G + G G GG A A V G P GAGG A I AG Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAG 110
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv3593	BLACTAMASEA	39	3e-05	Beta-lactamase class A signature.
>BLACTAMASEA#Beta-lactamase class A signature. Length = 286 Score = 39.0 bits (91), Expect = 3e-05 Identities = 24/137 (17%), Positives = 46/137 (33%), Gaps = 3/137 (2%) Query: 148 PSIASWRDVDAALSKTGARYSFQVAKVDNGRCDPVAGTNTGESLPLASIFKLYVLHALAG 207 S + + S+ R + ++D + E P+ S FK+ + A+ Sbjct: 21 ASPQPLEQIKLSESQLSGR--VGMIEMDLASGRTLTAWRADERFPMMSTFKVVLCGAVLA 78 Query: 208 AVQHNTVSWDDLLTVTAKSKAVGSSGLELPVGARVSVRTAAEKMIATSDNMATDLLIERL 267 V + + + S E + ++V I SDN A +LL+ + Sbjct: 79 RVDAGDEQLERKIHYRQQDLVDYSPVSEKHLADGMTVGELCAAAITMSDNSAANLLLATV 138 Query: 268 -GTRAIEEALASAGHHD 283 G + L G + Sbjct: 139 GGPAGLTAFLRQIGDNV 155
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv3595c	cloacin	39	4e-05	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 38.5 bits (89), Expect = 4e-05 Identities = 31/95 (32%), Positives = 36/95 (37%) Query: 152 GGSGGAAGLIGNGGSGGAGGAGAAGGSGGQGGLLYGNGGAGGNGGAATIPGGNGGAGGAG 211 G + GA GN G G G S G G N GG+G GG+G G G Sbjct: 8 GHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGG 67 Query: 212 GNAWLFGNGGAGGLGAAGAAGAAGVNPLTVPAGQG 246 G+G G L A A A G L+ P G Sbjct: 68 NGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG 102 Score = 37.4 bits (86), Expect = 1e-04 Identities = 34/108 (31%), Positives = 40/108 (37%), Gaps = 8/108 (7%) Query: 309 GGAGGIGGTGGEGGIGARGGTGGQGGMGGAGQPGVGGDAGDGGNGGIGGDGGAGGDGGAG 368 GG G TG G G G+GG G G + + GG G G G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 369 GAGGAGGLFGVSGSSGLGGAAGSGGNGGGGGEPGVAGSPGVGPAGRGG 416 G GG G GG +G+GGN P G P + G GG Sbjct: 63 GNGGGNG--------NSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG 102 Score = 35.1 bits (80), Expect = 6e-04 Identities = 33/93 (35%), Positives = 37/93 (39%), Gaps = 3/93 (3%) Query: 119 GADGTAPGQNGGAGGLLYGNGGNGAAG---VNAGIAGGSGGAAGLIGNGGSGGAGGAGAA 175 GA T+ NGG GL G G + +G N GGSG G G G GG G + Sbjct: 12 GAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNGNS 71 Query: 176 GGSGGQGGLLYGNGGAGGNGGAATIPGGNGGAG 208 GG G GG L G A G GG Sbjct: 72 GGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 34.7 bits (79), Expect = 7e-04 Identities = 35/106 (33%), Positives = 41/106 (38%), Gaps = 1/106 (0%) Query: 286 TGGTGGTGGAGGSGGRGGLLVGDGGAGGIGGTGGEGGIGARGGTGGQGGMGGAGQPGVGG 345 +GG G G G + G G G GG G + G G G G G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 346 DAGDGGNGGIGGDGGAGGDGGAGGAGGAGGLFGVSGSSGLGGAAGS 391 GGNG GG G GG+ A A A G +S + G GG A S Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALS-TPGAGGLAVS 106 Score = 31.2 bits (70), Expect = 0.010 Identities = 33/106 (31%), Positives = 39/106 (36%), Gaps = 6/106 (5%) Query: 130 GAGGLLYGNGGNGAAGVNAGIAGGSGGAAGLIGNGGSGGAGGAGAAGGSGGQGGLLYGNG 189 G G + G + +G G G G G + GSG + GG G G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGG--ASDGSGWSSENNPWGGGSGSGIHWGGGS 60 Query: 190 GAGGNGGAATIPGGNGGAGGAGGNAWLFGNGGAGGLGAAGAAGAAG 235 G G GG G +GG G GGN A G A GA G Sbjct: 61 GHGNGGGN----GNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG 102 Score = 30.5 bits (68), Expect = 0.013 Identities = 27/82 (32%), Positives = 29/82 (35%), Gaps = 1/82 (1%) Query: 267 TGGTG-GTGGTGLSVGGTGGTGGTGGTGGAGGSGGRGGLLVGDGGAGGIGGTGGEGGIGA 325 +GG G G S G G TG G G S G G + GG G GG Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 326 RGGTGGQGGMGGAGQPGVGGDA 347 G GG G GG G A Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSA 83 Score = 29.3 bits (65), Expect = 0.034 Identities = 29/91 (31%), Positives = 36/91 (39%) Query: 342 GVGGDAGDGGNGGIGGDGGAGGDGGAGGAGGAGGLFGVSGSSGLGGAAGSGGNGGGGGEP 401 G G + G G+ G G G G + G S ++ GG +GSG + GGG Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 402 GVAGSPGVGPAGRGGDGNLGQFGPEGAPGQP 432 G G G G G GNL A G P Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFGFP 93
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv3596c	HTHFIS	32	0.007	FIS bacterial regulatory protein HTH signature.
>HTHFIS#FIS bacterial regulatory protein HTH signature. Length = 484 Score = 32.5 bits (74), Expect = 0.007 Identities = 31/160 (19%), Positives = 59/160 (36%), Gaps = 28/160 (17%) Query: 518 IIGQEDAVKAVSKAIRRTRAGLKDPKRPSGSFIFAGPSGVGKTELSKALANFLFGDDDAL 577 ++G+ A++ + + + R + + + G SG GK +++AL ++ + Sbjct: 139 LVGRSAAMQEIYRVLARL-------MQTDLTLMITGESGTGKELVARALHDYGKRRNGPF 191 Query: 578 IQIDMGEFHDRFTASRLFGAPPGYVGYEEGGQLTEKVRRKP--FS-----VVLFDEIEKA 630 + I+M S LFG E G T R F + DEI Sbjct: 192 VAINMAAIPRDLIESELFGH--------EKGAFTGAQTRSTGRFEQAEGGTLFLDEIGDM 243 Query: 631 HQEIYNSLLQVLEDG---RLTDGQGRTVDFKNTVLIFTSN 667 + LL+VL+ G + D + ++ +N Sbjct: 244 PMDAQTRLLRVLQQGEYTTVGGRTPIRSDVR---IVAATN 280

Rv3609c

Rv3623

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
Rv3609c	2	13	-0.602130	GTP cyclohydrolase I FolE (GTP-ch-I)
Rv3610c	2	14	-0.627125	Membrane-bound protease FtsH (cell division
Rv3611	4	25	-1.705415	Hypothetical arginine and proline rich protein
Rv3612c	2	18	-2.395576	Conserved hypothetical protein
Rv3613c	1	17	-2.208713	Hypothetical protein
Rv3614c	0	15	-2.283062	ESX-1 secretion-associated protein EspD
Rv3615c	1	15	-2.185560	ESX-1 secretion-associated protein EspC
Rv3616c	2	13	-0.819625	ESX-1 secretion-associated protein A, EspA
Rv3617	1	14	-0.234916	Probable epoxide hydrolase EphA (epoxide
Rv3618	1	15	0.026028	Possible monooxygenase
Rv3619c	-1	15	0.426469	Putative ESAT-6 like protein EsxV (ESAT-6 like
Rv3620c	0	15	1.701422	Putative ESAT-6 like protein EsxW (ESAT-6 like
Rv3621c	-1	13	1.761225	PPE family protein PPE65
Rv3622c	2	13	1.890467	PE family protein PE32
Rv3623	2	13	1.584843	Probable conserved lipoprotein LpqG

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv3610c	HTHFIS	34	0.002	FIS bacterial regulatory protein HTH signature.
>HTHFIS#FIS bacterial regulatory protein HTH signature. Length = 484 Score = 34.4 bits (79), Expect = 0.002 Identities = 23/82 (28%), Positives = 32/82 (39%), Gaps = 18/82 (21%) Query: 199 VLLYGPPGTGKTLLARAV---AGEAGVPFFT-----ISGSDFVEMFVGV------GASRV 244 +++ G GTGK L+ARA+ PF I G GA Sbjct: 163 LMITGESGTGKELVARALHDYGKRRNGPFVAINMAAIPRDLIESELFGHEKGAFTGAQTR 222 Query: 245 RD-LFEQAKQNSPCIIFVDEID 265 FEQA+ + +F+DEI Sbjct: 223 STGRFEQAEGGT---LFLDEIG 241

Rv3649

Rv3657c

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
Rv3649	-1	12	3.271086	Probable helicase
Rv3650	0	15	3.812545	PE family protein PE33
Rv3651	-1	14	3.106811	Conserved hypothetical protein
Rv3652	6	22	6.418509	PE-PGRS family-related protein PE_PGRS60
Rv3653	5	16	6.152767	PE-PGRS family-related protein PE_PGRS61
Rv3654c	4	17	4.035701	Conserved hypothetical protein
Rv3655c	3	17	3.911398	Conserved hypothetical protein
Rv3656c	1	14	3.269523	Conserved hypothetical protein
Rv3657c	1	15	4.067727	Possible conserved alanine rich membrane

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv3652	RTXTOXINA	28	0.010	Gram-negative bacterial RTX toxin determinant A family...
>RTXTOXINA#Gram-negative bacterial RTX toxin determinant A family signature. Length = 1024 Score = 27.6 bits (61), Expect = 0.010 Identities = 10/49 (20%), Positives = 24/49 (48%), Gaps = 5/49 (10%) Query: 13 AAATDLATLGSTIGAANAAAA-----GSTTALLTAGADEVSAAIAAYSE 56 A+ T ++T+ +++ + +AAA G+ + L + + I S+ Sbjct: 366 ASLTTISTVLASVSSGISAAATTSLVGAPVSALVGAVTGIISGILEASK 414
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv3653	cloacin	32	0.001	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 32.0 bits (72), Expect = 0.001 Identities = 27/102 (26%), Positives = 38/102 (37%), Gaps = 7/102 (6%) Query: 17 NGANGAPGTGANGGDGGILFGSGGAGGSGAAGMAGGNGGAAGLFGNGGAGGAGGSATAGA 76 N + NGG G+ G G + GSG + GG +G + G G G+ Sbjct: 10 NTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNG 69 Query: 77 AGAGGNGGAGGL-------LFGTAGAGGNGGLSLGLGVAGGA 111 GG+G G L FG G L + ++ GA Sbjct: 70 NSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGA 111 Score = 31.6 bits (71), Expect = 0.002 Identities = 26/104 (25%), Positives = 36/104 (34%) Query: 49 MAGGNGGAAGLFGNGGAGGAGGSATAGAAGAGGNGGAGGLLFGTAGAGGNGGLSLGLGVA 108 M+GG+G + +G G T G G + G+G GG+G G + Sbjct: 1 MSGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGS 60 Query: 109 GGAGGAGGSGGSDTAGHGGTGGAGGLLFGAGEDGTTPGGNGGAG 152 G G G +G GG A G + G GG Sbjct: 61 GHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 30.8 bits (69), Expect = 0.003 Identities = 23/74 (31%), Positives = 28/74 (37%) Query: 109 GGAGGAGGSGGSDTAGHGGTGGAGGLLFGAGEDGTTPGGNGGAGGVAGLFGDGGNGGNAG 168 G GA + G+ G G G GG G+G GG+G G G+G G Sbjct: 8 GHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGG 67 Query: 169 VGTPAGNVGAGGTG 182 G G G GG Sbjct: 68 NGNSGGGSGTGGNL 81 Score = 29.3 bits (65), Expect = 0.010 Identities = 31/90 (34%), Positives = 36/90 (40%), Gaps = 4/90 (4%) Query: 74 AGAAGAGGNGGA----GGLLFGTAGAGGNGGLSLGLGVAGGAGGAGGSGGSDTAGHGGTG 129 +G G G N GA G + G G G GG S G G + GG GS GG+G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 130 GAGGLLFGAGEDGTTPGGNGGAGGVAGLFG 159 G G G+ GGN A FG Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAAPVAFG 91 Score = 28.5 bits (63), Expect = 0.017 Identities = 23/80 (28%), Positives = 27/80 (33%) Query: 20 NGAPGTGANGGDGGILFGSGGAGGSGAAGMAGGNGGAAGLFGNGGAGGAGGSATAGAAGA 79 +G G G N G G G +G N GG+G G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 80 GGNGGAGGLLFGTAGAGGNG 99 GNGG G G +G GGN Sbjct: 62 HGNGGGNGNSGGGSGTGGNL 81
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv3656c	MECHCHANNEL	24	0.030	Bacterial mechano-sensitive ion channel signature.
>MECHCHANNEL#Bacterial mechano-sensitive ion channel signature. Length = 136 Score = 24.4 bits (53), Expect = 0.030 Identities = 14/37 (37%), Positives = 21/37 (56%), Gaps = 1/37 (2%) Query: 27 VEYAIGTIAAAAFGAILYTVVTGDSIVSALNRIIGRA 63 V+ A+G I AAFG I+ + + D I+ L +IG Sbjct: 17 VDLAVGVIIGAAFGKIV-SSLVADIIMPPLGLLIGGI 52

Rv3816c

Rv3826

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
Rv3816c	-2	13	-3.018356	Possible acyltransferase
Rv3817	-1	17	-3.629206	Possible phosphotransferase
Rv3818	-1	19	-4.964611	Unknown protein
Rv3819	-1	24	-5.753242	Unknown protein
Rv3820c	-2	17	-3.416940	Possible conserved polyketide synthase
Rv3821	-2	18	-3.628299	Probable conserved integral membrane protein
Rv3822	-2	17	-3.384143	Conserved hypothetical protein
Rv3823c	-2	17	-3.303273	Conserved integral membrane transport protein
Rv3824c	-3	16	-2.606487	Conserved polyketide synthase associated protein
Rv3825c	-3	14	-1.809063	Polyketide synthase Pks2
Rv3826	-1	16	-3.377359	Probable fatty-acid-AMP ligase FadD23

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv3823c	ACRIFLAVINRP	53	9e-09	Acriflavin resistance protein family signature.
>ACRIFLAVINRP#Acriflavin resistance protein family signature. Length = 1034 Score = 52.5 bits (126), Expect = 9e-09 Identities = 42/240 (17%), Positives = 91/240 (37%), Gaps = 36/240 (15%) Query: 205 ADLNLTGQRDRSR-IEFAI----------TILLLVILLIIYGNPITMVLPLITIGMSVVV 253 + + D + ++ +I +L+ +++ + N ++P I + + + Sbjct: 319 QGMKVLYPYDTTPFVQLSIHEVVKTLFEAIMLVFLVMYLFLQNMRATLIPTIAVPVVL-- 376 Query: 254 AQRLVAIAGLAGLGIANQSIIFMSGMMVGAGT--DYAVFLISRYHDYLR-QGADSDQAVK 310 L A LA G + + + M GM++ G D A+ ++ + +A + Sbjct: 377 ---LGTFAILAAFGY-SINTLTMFGMVLAIGLLVDDAIVVVENVERVMMEDKLPPKEATE 432 Query: 311 KALTSIGKVIAASAATVAITFLGMVFT--QLG-ILKTVGPMLGISVAVVFFAAVTLLPAL 367 K+++ I + A ++ F+ M F G I + + ++A+ A+ L PAL Sbjct: 433 KSMSQIQGALVGIAMVLSAVFIPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPAL 492 Query: 368 MVL-------------TGRRGWIAPRRDLTRRFWRSSGVHIVRRPKTHLLASALVLVILA 414 G GW D + + +S I+ +LL AL++ + Sbjct: 493 CATLLKPVSAEHHENKGGFFGWFNTTFDHSVNHYTNSVGKILGSTGRYLLIYALIVAGMV 552

Rv3896c

Rv3901c

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
Rv3896c	2	19	1.837613	Conserved hypothetical protein
Rv3897c	2	24	-0.299476	Conserved hypothetical protein
Rv3898c	2	23	-0.525298	Conserved hypothetical protein
Rv3899c	2	23	-0.471255	Conserved hypothetical protein
Rv3900c	3	25	-1.669502	Conserved hypothetical alanine rich protein
Rv3901c	2	21	-1.201454	Possible membrane protein

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv3897c	60KDINNERMP	28	0.029	60kDa inner membrane protein signature.
>60KDINNERMP#60kDa inner membrane protein signature. Length = 548 Score = 28.0 bits (62), Expect = 0.029 Identities = 11/37 (29%), Positives = 21/37 (56%), Gaps = 1/37 (2%) Query: 17 VGGVMGPLTQLPQQAMQAGQGAMQPLMSALQQTYGAE 53 V G+M PLT+ +M + +QP + A+++ G + Sbjct: 365 VRGIMYPLTKAQYTSMAKMR-MLQPKIQAMRERLGDD 400
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv3899c	PRTACTNFAMLY	30	0.027	Pertactin virulence factor family signature.
>PRTACTNFAMLY#Pertactin virulence factor family signature. Length = 910 Score = 29.6 bits (66), Expect = 0.027 Identities = 43/188 (22%), Positives = 60/188 (31%), Gaps = 20/188 (10%) Query: 2 VTGQPAAAGAHSLSEGAMTAMQSGSVPPPQAT---PPITTPPVVSAPTMAAGIEATHGPV 58 VT Q +A L GA+ ++Q +PP + +T P AP + + A+ + Sbjct: 171 VTVQRSAIVDGGLHIGALQSLQPEDLPPSRVVLRDTNVTAVPASGAPAAVSVLGASELTL 230 Query: 59 DTPANTSG-APPASTGTTGPVAPTVVTAGPVAAPAAPVVGGSAVPAGPLPAYGSDLRPPV 117 D T G A + V T APA V G AVP G +P Sbjct: 231 DGGHITGGRAAGVAAMQGAVVHLQRATIRRGDAPAGGAVPGGAVPGGAVPGGFGP----- 285 Query: 118 VAAPAVPSVPTAPVSGAPVAPSASSAPSAGGALVSPVERAASKAVAGQAGASSSTMAGAS 177 V S SS A + A A + G + Sbjct: 286 ------GGFGPVLDGWYGVDVSGSSVELAQSIV-----EAPELGAAIRVGRGARVTVSGG 334 Query: 178 ALSATAGA 185 +LSA G Sbjct: 335 SLSAPHGN 342

Rv0273c

Rv0279c

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
Rv0273c	-2	11	-1.096465	Possible transcriptional regulatory protein
Rv0274	5	17	5.346379	Conserved protein
Rv0275c	10	22	8.112180	Possible transcriptional regulatory protein
Rv0276	11	19	7.740593	Conserved hypothetical protein
Rv0277c	11	21	7.750325	Possible toxin VapC25. Contains PIN domain.
Rv0278c	6	15	6.101805	PE-PGRS family protein PE_PGRS3
Rv0279c	0	10	2.922633	PE-PGRS family protein PE_PGRS4

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv0273c	HTHTETR	54	2e-11	TetR bacterial regulatory protein HTH signature.
>HTHTETR#TetR bacterial regulatory protein HTH signature. Length = 215 Score = 54.2 bits (130), Expect = 2e-11 Identities = 27/173 (15%), Positives = 52/173 (30%), Gaps = 8/173 (4%) Query: 1 MPDFPTQRGRRTQAAIDAAARTVVVRNGILATTVADITAEAGRSAASFYNYYDSKEAMVR 60 M Q + T+ I A + + G+ +T++ +I AG + + Y ++ K + Sbjct: 1 MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFS 60 Query: 61 QWALRFRDDANQRALSVIRHGLSDRERAYEAAAAHWYTY-----RNRLAEAISVSQLAMV 115 + + + L D H R RL I + V Sbjct: 61 EIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVTEERRRLLMEIIFHKCEFV 120 Query: 116 SDDF--AQYWSEICQIPISFITETVKRAQAHGYCVGD-DPQLMAEAIVAMFNQ 165 + Q +C I +T+K D + A + + Sbjct: 121 GEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMTRRAAIIMRGYISG 173
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv0275c	HTHTETR	44	1e-07	TetR bacterial regulatory protein HTH signature.
>HTHTETR#TetR bacterial regulatory protein HTH signature. Length = 215 Score = 43.8 bits (103), Expect = 1e-07 Identities = 29/195 (14%), Positives = 60/195 (30%), Gaps = 13/195 (6%) Query: 14 AERLATRRRQSLSAGLDLLGSDQHDIAELTIRTICRRAGLSVRYFYESFTDKDEFVGRVF 73 + R+ L L L Q ++ ++ I + AG++ Y F DK + ++ Sbjct: 6 KQEAQETRQHILDVALRLF--SQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSEIW 63 Query: 74 DWVVAELVATTQAAVTAVPA--REQTRAGMANIVRTITADARVGRLL-FSTQLANAVITR 130 + + + P R + +++ + + R L+ V Sbjct: 64 ELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVTEERRRLLMEIIFHKCEFVGEM 123 Query: 131 -KRAESSALFAMLSGQHAVDTLHA-------PANDHVKAVAHFAVGGVGQTISAWLAGDV 182 ++ + S TL PA+ + A G + + WL Sbjct: 124 AVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMTRRAAIIMRGYISGLMENWLFAPQ 183 Query: 183 RLDPDQLVDQLAALL 197 D + A+L Sbjct: 184 SFDLKKEARDYVAIL 198
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv0278c	cloacin	44	2e-06	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 44.3 bits (104), Expect = 2e-06 Identities = 42/115 (36%), Positives = 53/115 (46%), Gaps = 3/115 (2%) Query: 117 NGANGAPGTGANGGDGGWLIGNGGAGGSGAAGVNGGAGGNGGAGGNGGAGGLIGNGGAGG 176 N + NGG G +G G + GSG + N GG G+G + G G GNGG G Sbjct: 10 NTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNG 69 Query: 177 AGGVASSGIGGSGGAGGNAMLFG--AGGAGGAGGGVVALTGGAGGAGGAGGNAGL 229 G SG GG+ A + FG A GAGG V+++ GA A A A L Sbjct: 70 NSG-GGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAAIADIMAAL 123 Score = 38.2 bits (88), Expect = 2e-04 Identities = 35/114 (30%), Positives = 41/114 (35%), Gaps = 6/114 (5%) Query: 143 GSGAAGVNGGAGGNGGAGGNGGAGGLIGNGGAGGAGGVASSGIGGSGGAGGNAMLFGAGG 202 G G N GA G NGG GL GGA G +S GG+G G G Sbjct: 3 GGDGRGHNTGAHSTSG-NINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 203 AGGAGGGVVALTGGAGGAGGAGGNAGLLFG-----AAGVGGAGGFTNGSALGGA 251 G GG + G G + A + FG G GG + AL A Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAA 115 Score = 36.2 bits (83), Expect = 7e-04 Identities = 38/104 (36%), Positives = 43/104 (41%), Gaps = 9/104 (8%) Query: 722 GTGGAGTNFGAGGNGGN--GGLFGAGGTGGAAGSGGSGITTGGGGHGGNAGLLSLGASGG 779 G G G N GA GN GG G G GGA+ G G G +G+ G SG Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 780 AGGSGGASSLAGGAGGTGGNG-----ALLFGFRGAGGAGGHGGA 818 G G +S GG GTGGN + FGF G G A Sbjct: 63 GNGGGNGNS--GGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 35.8 bits (82), Expect = 7e-04 Identities = 31/110 (28%), Positives = 41/110 (37%), Gaps = 3/110 (2%) Query: 687 AGGNGGLFANGGAGGAGGFNAAGGNGGNGGLFGTGGTGGAGTNFGAGGNGGNGGLFGAGG 746 +GG+G G +G N G GG G + N GG+G G G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 747 TGGAAGSGGSGITTGGGGHGGNAGL---LSLGASGGAGGSGGASSLAGGA 793 G G+G SG +G GG+ A G G A S++ GA Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGA 111 Score = 34.7 bits (79), Expect = 0.002 Identities = 34/111 (30%), Positives = 43/111 (38%), Gaps = 5/111 (4%) Query: 248 LGGAGGAGGAGGLFATGGV--GGSGGAGSSGGAGGAGGAGGL---FGAGGTGGHGGFADS 302 + G G G G +T G GG G G GGA G +G G G S Sbjct: 1 MSGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGS 60 Query: 303 SFGGVGGAGGAGGLFGAGGEGGSGGHSLVAGGDGGAGGNAGMLALGAAGGA 353 G GG G +GG G GG + + G + AG LA+ + GA Sbjct: 61 GHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGA 111 Score = 34.3 bits (78), Expect = 0.002 Identities = 21/71 (29%), Positives = 25/71 (35%) Query: 671 TGGHGAAGGVPAGVGGAGGNGGLFANGGAGGAGGFNAAGGNGGNGGLFGTGGTGGAGTNF 730 TG H +G + G G G GG G G G G+G G G Sbjct: 11 TGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNGN 70 Query: 731 GAGGNGGNGGL 741 GG+G G L Sbjct: 71 SGGGSGTGGNL 81 Score = 34.3 bits (78), Expect = 0.002 Identities = 31/87 (35%), Positives = 36/87 (41%), Gaps = 3/87 (3%) Query: 368 IGGAGGAGGNAGLLFGSGG-SGGAGGFGFADGGQGGPGGNAGTVFGSGGAGGNGGVGQGF 426 + G G G N G SG +GG G G G G G ++ GG+G G G Sbjct: 1 MSGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGS 60 Query: 427 AGGIGGAGGTPGLIGNGGNGGNGGASA 453 G GG G G G G GGN A A Sbjct: 61 GHGNGGGNGNSG--GGSGTGGNLSAVA 85 Score = 33.9 bits (77), Expect = 0.004 Identities = 34/110 (30%), Positives = 42/110 (38%), Gaps = 7/110 (6%) Query: 215 GGAGGAGGAGGNAGLLFGAAGVGGAGGFTNGSALGG-----AGGAGGAGGLFATGGVGGS 269 G GA GN G G+G GG ++GS GG+G G G Sbjct: 8 GHNTGAHSTSGNIN--GGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNG 65 Query: 270 GGAGSSGGAGGAGGAGGLFGAGGTGGHGGFADSSFGGVGGAGGAGGLFGA 319 GG G+SGG G GG A G + GG+ + AG L A Sbjct: 66 GGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAA 115 Score = 33.9 bits (77), Expect = 0.004 Identities = 37/134 (27%), Positives = 51/134 (38%) Query: 263 TGGVGGSGGAGSSGGAGGAGGAGGLFGAGGTGGHGGFADSSFGGVGGAGGAGGLFGAGGE 322 +GG G G+ +G G G GG G S GG G+G +G G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 323 GGSGGHSLVAGGDGGAGGNAGMLALGAAGGAGGIGGDGGTLTAGGIGGAGGAGGNAGLLF 382 G+GG + +GG G GGN +A A G + G A I + A ++ Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAAIADIMA 121 Query: 383 GSGGSGGAGGFGFA 396 G G +G A Sbjct: 122 ALKGPFKFGLWGVA 135 Score = 32.0 bits (72), Expect = 0.012 Identities = 37/113 (32%), Positives = 41/113 (36%), Gaps = 24/113 (21%) Query: 357 GGDGGTLTAGGIGGAGGAGGNAGLLFGSGGSGGAGGFGFADGGQGGPGGNAGTVFGSGGA 416 GGDG G GA GN +GG G G G GSG + Sbjct: 3 GGDG----RGHNTGAHSTSGN------------------INGGPTGLGVGGGASDGSGWS 40 Query: 417 GGNGGVGQGFAGGIGGAGGTPGLIGNGGNGGNGGASAVTGGNGGIGGTGVLIG 469 N G G GI GG+ GNGG GN G + TGGN V G Sbjct: 41 SENNPWGGGSGSGIHWGGGSGH--GNGGGNGNSGGGSGTGGNLSAVAAPVAFG 91 Score = 31.6 bits (71), Expect = 0.017 Identities = 33/103 (32%), Positives = 38/103 (36%), Gaps = 2/103 (1%) Query: 751 AGSGGSGITTGGGGHGGN--AGLLSLGASGGAGGSGGASSLAGGAGGTGGNGALLFGFRG 808 +G G G TG GN G LG GGA G SS GG G+G G G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 809 AGGAGGHGGAALTSIQQGGAGGAGGNGGLLFGSAGAGGAGGSG 851 G GG+G + S G F + GAGG Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 31.6 bits (71), Expect = 0.019 Identities = 36/116 (31%), Positives = 43/116 (37%), Gaps = 8/116 (6%) Query: 235 GVGGAGGFTNGSALGGAGGAGGAGGLFATGGVGGSGGAGSSGGAGGAGGAGGLFGAGGTG 294 G T+G+ GG G G GG A+ G G S GG G+G G G G Sbjct: 8 GHNTGAHSTSGNINGGPTGLGVGGG--ASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNG 65 Query: 295 GHGGFADSSFGGVGGAGGAGGLFGAGGEGGSGGHSLVAGGDGGAGGNAGMLALGAA 350 G G GG+G G L G +L G GG + AL AA Sbjct: 66 GGNG------NSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAA 115 Score = 30.8 bits (69), Expect = 0.027 Identities = 35/111 (31%), Positives = 43/111 (38%), Gaps = 4/111 (3%) Query: 290 AGGTG-GHGGFADSSFGGVGGAGGAGGLFGAGGEGGSGGHSLVAGGDGGAGGNAGMLALG 348 +GG G GH A S+ G + G G+ G G GSG S GG+G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGV-GGGASDGSGWSSENNPWGGGSGSGIHWGGGS 60 Query: 349 AAGGAGGIGGDGGTLTAGGIGGAGGAGGNAGLLFGSGGSGGAGGFGFADGG 399 G GG G GG GG A A G F + + GAGG + Sbjct: 61 GHGNGGGNGNSGGGSGTGGNLSAVAAPVAFG--FPALSTPGAGGLAVSISA 109 Score = 30.8 bits (69), Expect = 0.030 Identities = 33/104 (31%), Positives = 42/104 (40%), Gaps = 5/104 (4%) Query: 575 GNGGNGGHGATNTAATATGGAGGAGGILFGTGGNGGTGGIATGAGGIGGAGGAGGVSLLI 634 G+G GA +T+ GG G G G G + G+G + GG G+G Sbjct: 4 GDGRGHNTGAHSTSGNINGGPTGLGV---GGGASDGSGWSSENNPW-GGGSGSGIHWGGG 59 Query: 635 GSGGTGGNGGNSIGVAGIGGAGGRGGDAGLLFGAAGTGGHGAAG 678 G GG GNS G +G GG A + FG GA G Sbjct: 60 SGHGNGGGNGNSGGGSGTGG-NLSAVAAPVAFGFPALSTPGAGG 102 Score = 30.8 bits (69), Expect = 0.030 Identities = 28/93 (30%), Positives = 35/93 (37%), Gaps = 2/93 (2%) Query: 338 AGGNAGMLALGAAGGAGGIGGDGGTLTAGGIGGAGGAGGNAGLLFGSGGSGGAGGFGFAD 397 +GG+ GA +G I G L GG G + +G G G G + Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 398 GGQGGPGGNAGTVFGSGGAGGNGGVGQGFAGGI 430 G GG GN+G GSG G V A G Sbjct: 62 HGNGGGNGNSGG--GSGTGGNLSAVAAPVAFGF 92 Score = 30.5 bits (68), Expect = 0.039 Identities = 29/95 (30%), Positives = 38/95 (40%), Gaps = 9/95 (9%) Query: 779 GAGGSGGASSLAGGAGGTGGNGALLFGFRGAGGAGGHGGAALTSIQQGGAGGAGGNGGLL 838 G G + GA S +G G G G GG + S + GG G+G Sbjct: 6 GRGHNTGAHSTSGNINGGPT---------GLGVGGGASDGSGWSSENNPWGGGSGSGIHW 56 Query: 839 FGSAGAGGAGGSGANALGAGTGGTGGDGGHAGVFG 873 G +G G GG+G + G+GTGG FG Sbjct: 57 GGGSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFG 91
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv0279c	cloacin	38	1e-04	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 38.2 bits (88), Expect = 1e-04 Identities = 31/102 (30%), Positives = 41/102 (40%), Gaps = 4/102 (3%) Query: 117 NGTNGAPGTGANGGDGGWLIGNGGAGGSGAAGVN----GGAGGNGGAGGLIGNGGAGGAG 172 N + NGG G +G G + GSG + N GG+G GG G+G GG G Sbjct: 10 NTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNG 69 Query: 173 GRASTGTGGAGGAGGAAGMLFGAAGVGGPGGFAAAFGATGGA 214 G + AA + FG + PG A + GA Sbjct: 70 NSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGA 111 Score = 36.6 bits (84), Expect = 4e-04 Identities = 32/107 (29%), Positives = 45/107 (42%), Gaps = 4/107 (3%) Query: 628 GTGGLFANGGAGGAGGFGANGGTGGNGLLFGAGGTGGAGTLGADGGAGGHGGLFGAGGTG 687 G G N GA G G TG + G+G + GG G G +G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 688 GAGGSSGGTFGGNGGSGGNAGLLALGASG----GAGGSGGSALNVGG 730 G GG +G + GG+G G + + A A G G+GG A+++ Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISA 109 Score = 35.8 bits (82), Expect = 6e-04 Identities = 30/82 (36%), Positives = 38/82 (46%) Query: 283 AGGTGGSGGTSIINVGGNGGAGGDAGMLSLGAAGGAGGSGGSNPDGGGGAGGIGGDGGTL 342 +GG G T + GN G + GA+ G+G S +NP GGG GI GG+ Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 343 FGSGGAGGVCGLGFDAGGAGGA 364 G+GG G G G GG A Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSA 83 Score = 34.7 bits (79), Expect = 0.002 Identities = 26/78 (33%), Positives = 33/78 (42%) Query: 693 SGGTFGGNGGSGGNAGLLALGASGGAGGSGGSALNVGGTGGVGGNGGSGGSLFGFGGAGG 752 SGG G+ + G G G GG++ G + GG GS +GG G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 753 TGGSSGIGSSGGTGGDGG 770 G G G+SGG G GG Sbjct: 62 HGNGGGNGNSGGGSGTGG 79 Score = 33.9 bits (77), Expect = 0.003 Identities = 29/110 (26%), Positives = 40/110 (36%), Gaps = 2/110 (1%) Query: 211 TGGAGGAGGNGGLFADGGVGGAGGATDAGTGGAGGSG--GNGGLFGAGGTGGPGGFGIFG 268 +GG G G G + G G G + GSG +G G G G G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 269 GGAGGDGGSGGLFGAGGTGGSGGTSIINVGGNGGAGGDAGMLSLGAAGGA 318 G GG G+ G G S + + G + AG L++ + GA Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGA 111 Score = 33.5 bits (76), Expect = 0.003 Identities = 32/95 (33%), Positives = 37/95 (38%), Gaps = 9/95 (9%) Query: 682 GAGGTGGAGGSSGGTFGGNGGSGGNAGLLALGASGGAGGSGGSALNVGGTGGVGGNGGSG 741 G G GA +SG GG G G G AS G+G S + GG+G GG Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGG-----ASDGSGWSSENNPWGGGSGSGIHWGGGS 60 Query: 742 GSLFGFGGAGGTGGSSGIGSSGGTGGDGGTAGVFG 776 G G GG G S G +GG FG Sbjct: 61 GH----GNGGGNGNSGGGSGTGGNLSAVAAPVAFG 91 Score = 32.8 bits (74), Expect = 0.006 Identities = 33/105 (31%), Positives = 41/105 (39%), Gaps = 1/105 (0%) Query: 659 AGGTGGAGTLGADGGAGGHGGLFGAGGTGGAGGSSGGTFGGNGGSGGNAGLLALGASGGA 718 +GG G GA +G G G GG G N GG +G G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 719 GGSGGSALNVGGTGGVGGNGGSGGSLFGFG-GAGGTGGSSGIGSS 762 G+GG N GG G GGN + + FG A T G+ G+ S Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVS 106 Score = 32.8 bits (74), Expect = 0.007 Identities = 31/93 (33%), Positives = 37/93 (39%), Gaps = 2/93 (2%) Query: 377 AGGAGGGSFAGAGGTGGA--GGAPGLVGNAGNGGNGGASANGAGAAGGAGGSGVLIGNGG 434 +GG G G GA T G GG GL G G S+ GG+G G G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 435 NGGSGGTGAPAGTAGAGGLGGQLLGRDGFNAPA 467 +G GG G G +G GG + F PA Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPA 94 Score = 32.4 bits (73), Expect = 0.008 Identities = 32/120 (26%), Positives = 38/120 (31%), Gaps = 12/120 (10%) Query: 219 GNGGLFADGGVGGAGGATDAGTGGAGGSGGNGGLFGAGGTGGPGGFGIFGGGAGGDGGSG 278 G G + G G + G G G GG G P G GG G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWG--------GGSGSGI 54 Query: 279 GLFGAGGTGGSGGTSIINVGGNGGAGGDAGMLSLGAAGGAGGSGGSNPDGGGGAGGIGGD 338 G G G GG N GG+G + ++ A G S P GG A I Sbjct: 55 HWGGGSGHGNGGG----NGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAG 110 Score = 32.4 bits (73), Expect = 0.009 Identities = 32/101 (31%), Positives = 38/101 (37%) Query: 575 SGGTGGSGGFGLDTGGAGGRGGDAGLFLGAAGTGGQAALSQNFIGAGGTAGAGGTGGLFA 634 SGG G G + GG GL +G + G S+N GG+ GG Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 635 NGGAGGAGGFGANGGTGGNGLLFGAGGTGGAGTLGADGGAG 675 +G GG G G GTGGN A G L G G Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG 102 Score = 32.0 bits (72), Expect = 0.010 Identities = 37/132 (28%), Positives = 46/132 (34%), Gaps = 16/132 (12%) Query: 242 GAGGSGGNGGLFGAGGT--GGPGGFGIFGGGAGGDGGSGGLFGAGGTGGSGGTSIINVGG 299 G G G N G G GGP G G+ GG + G G S GG GSG I+ GG Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSG----IHWGG 58 Query: 300 NGGAGGDAGMLSLGAAGGAGGSGGSNPDGGGGAGGIGGDGGTLFGSGGAGGVCGLGFDAG 359 G G GG G+ G GG + F + G GL Sbjct: 59 GSGHGN----------GGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSIS 108 Query: 360 GAGGAGGKAGLL 371 + A ++ Sbjct: 109 AGALSAAIADIM 120 Score = 32.0 bits (72), Expect = 0.011 Identities = 39/135 (28%), Positives = 47/135 (34%), Gaps = 10/135 (7%) Query: 139 GGAGGSGAAGVNGGAGGNGGAGGLIGNGGAGGAGGRASTGTGGAGGAGGAAGMLFGAAGV 198 G G + NGG GL GGA G +S GG+G G+ Sbjct: 4 GDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHG 63 Query: 199 GGPGGFAAAFGATGGAGGAGGNGGLFADGGVGGAGGATDAGTGGAGGSGGNGGLFGA--- 255 G G G +GG G GGN A G + G GG S G L A Sbjct: 64 NGGGN-----GNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAAIAD 118 Query: 256 --GGTGGPGGFGIFG 268 GP FG++G Sbjct: 119 IMAALKGPFKFGLWG 133 Score = 32.0 bits (72), Expect = 0.013 Identities = 31/114 (27%), Positives = 39/114 (34%), Gaps = 5/114 (4%) Query: 171 AGGRASTGTGGAGGAGGAAGMLFGAAGVGGPGGFAAAFGATGGAGGAGGNGGLFADGGVG 230 +GG GA G GVGG + + + G G G+ GG G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 231 GAGGATDAGTGGAGGSGGNGGLFGAGGTGGPGGFGIFGGGAGGDGGSGGLFGAG 284 G + +GG G+GGN A P FG G GG AG Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAA-----PVAFGFPALSTPGAGGLAVSISAG 110 Score = 31.2 bits (70), Expect = 0.017 Identities = 35/110 (31%), Positives = 44/110 (40%), Gaps = 14/110 (12%) Query: 619 GAGGTAGAGGTGGLFANGGAGGAGGFGANGGTG----------GNGLLFGAGGTGGAGTL 668 G G GA T G G G G GA+ G+G G+G GG G G Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNG 65 Query: 669 GADGGAGGHGGLFGAGGTGGAGGSSGGTFGGNGGSGGNAGLLALGASGGA 718 G +G +GG G G + ++ FG S AG LA+ S GA Sbjct: 66 GGNGNSGGGSG----TGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGA 111 Score = 31.2 bits (70), Expect = 0.018 Identities = 25/79 (31%), Positives = 33/79 (41%), Gaps = 3/79 (3%) Query: 353 GLGFDAGGAGGAGGKAGLLIGAGGAGGAGGGSFAGAGGT---GGAGGAPGLVGNAGNGGN 409 G G + G +G G G G GGA GS + GG+G G +G+G Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNG 65 Query: 410 GGASANGAGAAGGAGGSGV 428 GG +G G+ G S V Sbjct: 66 GGNGNSGGGSGTGGNLSAV 84 Score = 30.5 bits (68), Expect = 0.036 Identities = 34/121 (28%), Positives = 41/121 (33%), Gaps = 5/121 (4%) Query: 640 GAGGFGANGGTGGNGLLFGAGGTGGAGTLGADGGAG-GHGGLFGAGGTGGAGGSSGGTFG 698 G G G N G G TG GA G+G GG+G GG+ Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 699 GNGGSGGNAGLLALGASGGAGGSGGSALNVGGTGGVGGNGGSGGSLFGFGGAGGTGGSSG 758 GNGG GN+G G SG G A V G+GG + + Sbjct: 63 GNGGGNGNSG----GGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAAIAD 118 Query: 759 I 759 I Sbjct: 119 I 119 Score = 30.1 bits (67), Expect = 0.041 Identities = 33/113 (29%), Positives = 40/113 (35%), Gaps = 2/113 (1%) Query: 531 GGDGGSGGAGGILSGIGGTGGSGGIGTTGQGGTGGTGGAALLIGSGGTG-GSGGFGLDTG 589 GGDG G + GG G+G G G + GG+G G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 590 GAGGRGGDAGLFLGAAGTGGQAALSQNF-IGAGGTAGAGGTGGLFANGGAGGA 641 G GG G++G G G A F A T GAGG + G A Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAA 115

Rv0450c

Rv0456A

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
Rv0450c	1	15	-4.048247	Probable conserved transmembrane transport
Rv0451c	0	13	-1.160541	Probable conserved membrane protein MmpS4
Rv0452	1	13	-0.215699	Possible transcriptional regulatory protein
Rv0453	1	14	-0.948137	PPE family protein PPE11
Rv0454	-1	14	-2.308502	Conserved hypothetical protein
Rv0455c	-2	12	-1.579471	Conserved protein
Rv0456c	-2	12	-0.598568	enoyl-CoA hydratase EchA2 (enoyl hydrase)
Rv0456A	-2	13	-0.857009	Possible toxin MazF1

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv0450c	ACRIFLAVINRP	50	4e-08	Acriflavin resistance protein family signature.
>ACRIFLAVINRP#Acriflavin resistance protein family signature. Length = 1034 Score = 50.2 bits (120), Expect = 4e-08 Identities = 41/206 (19%), Positives = 74/206 (35%), Gaps = 13/206 (6%) Query: 727 IKSIDAIRTAAEESLKGTPLEDAKIYLAGTAAVFHDIS-EGAQWDLLIAAISSLCLIFII 785 + + AI+ A L+ + K+ F +S L A + L+F++ Sbjct: 300 LDTAKAIK-AKLAELQPFFPQGMKVLYPYDTTPFVQLSIHEVVKTLFEAIM----LVFLV 354 Query: 786 MLIITRAFIAAAVIVGTVALSLGASFGLSVLLWQHILAIHLHWLVLAMSVIVLLAVGSDY 845 M + + A + V + L +F + I + + +VLA+ ++V A+ Sbjct: 355 MYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINTLTMFGMVLAIGLLVDDAIVVVE 414 Query: 846 NLLLVSRFKQEIGAGLKTGIIRSMGGTGKVVTNAGLVFAVT---MASMAVSDLRVIGQVG 902 N V R E K +SM + +V + MA S + Q Sbjct: 415 N---VERVMMEDKLPPKEATEKSMSQIQGALVGIAMVLSAVFIPMAFFGGSTGAIYRQFS 471 Query: 903 TTIGLGLLFDTLIVRSFMTPSIAALL 928 TI + L+ TP++ A L Sbjct: 472 ITIVSAMALSVLVALIL-TPALCATL 496 Score = 45.6 bits (108), Expect = 1e-06 Identities = 42/238 (17%), Positives = 83/238 (34%), Gaps = 32/238 (13%) Query: 208 VAVIFIMLLLVYRSIITVVLLLITVGVELTAARGVVAVLGHSGAIGLTTFAVSLLTSLAI 267 + ++F+++ L +++ ++ I V V L ++A G+S LT F + L AI Sbjct: 348 IMLVFLVMYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINT-LTMFGMVL----AI 402 Query: 268 AAGTDYGIFIIGRYQEARQAGED--KEAAYYTMYRGTAHVILGSGLTIAGA---TFCLSF 322 D I ++ + + KEA +M ++G + ++ Sbjct: 403 GLLVDDAIVVVENVERVMMEDKLPPKEATEKSM-SQIQGALVGIAMVLSAVFIPMAFFGG 461 Query: 323 ARMPYFQTLGIPCAVGMLVAVAVALTLGPAVLHV-------------GSRFGLFDPKRLL 369 + ++ I M ++V VAL L PA+ G FG F+ Sbjct: 462 STGAIYRQFSITIVSAMALSVLVALILTPALCATLLKPVSAEHHENKGGFFGWFNTTFDH 521 Query: 370 KVRGWRRVGTVVVRWPLPVLVATCAIALVGLLALPGYKTSYNDRDYLPDFIPANQGYA 427 V + ++ L+ ++A +LP+ +QG Sbjct: 522 SVNHYTNSVGKILGSTGRYLL-----IYALIVAGMVVLFLRLPSSFLPEE---DQGVF 571 Score = 38.7 bits (90), Expect = 1e-04 Identities = 45/214 (21%), Positives = 84/214 (39%), Gaps = 15/214 (7%) Query: 153 QGTPLANESVEAVRSIVESTPA--PPGIKAYVTGPSALAADMHHSGDRSMARITMVTVAV 210 QG S +++E+ + P GI TG ++ SG+++ A + ++ V Sbjct: 827 QGEAAPGTSSGDAMALMENLASKLPAGIGYDWTG---MSYQERLSGNQAPA-LVAISFVV 882 Query: 211 IFIMLLLVYRSIITVVLLLITVGVELTAARGVVAVLGHSGAIGLTTFAVSLLTSLAIAAG 270 +F+ L +Y S V +++ V L ++A + + F V LLT++ ++A Sbjct: 883 VFLCLAALYESWSIPVSVMLV--VPLGIVGVLLAATLFNQKNDV-YFMVGLLTTIGLSAK 939 Query: 271 TDYGIFIIGRYQEARQA-GEDKEAAYYTMYRGTAHVILGSGLTIAGATFCLSFARMP--- 326 I I+ ++ + G+ A R IL + L L+ + Sbjct: 940 N--AILIVEFAKDLMEKEGKGVVEATLMAVRMRLRPILMTSLAFILGVLPLAISNGAGSG 997 Query: 327 YFQTLGIPCAVGMLVAVAVALTLGPAVLHVGSRF 360 +GI GM+ A +A+ P V R Sbjct: 998 AQNAVGIGVMGGMVSATLLAIFFVPVFFVVIRRC 1031
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv0452	HTHTETR	67	1e-15	TetR bacterial regulatory protein HTH signature.
>HTHTETR#TetR bacterial regulatory protein HTH signature. Length = 215 Score = 67.0 bits (163), Expect = 1e-15 Identities = 20/141 (14%), Positives = 51/141 (36%), Gaps = 3/141 (2%) Query: 16 RTEENKRQRAAALVEAARSLALETGVASVTLTAVAGRAGIHYSAVRRYFTSHKEVLLHLA 75 +T++ ++ +++ A L + GV+S +L +A AG+ A+ +F ++ + Sbjct: 4 KTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSEIW 63 Query: 76 AEGWARWSGTVCEQLGEPGPMSAPRVAEALANGLAAD---PLFCDLLANLHLHLEQEVDV 132 + E + + E L + L + L+ + E ++ Sbjct: 64 ELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVTEERRRLLMEIIFHKCEFVGEM 123 Query: 133 DRVIEVKRTSIAAVIALVDAI 153 V + +R ++ Sbjct: 124 AVVQQAQRNLCLESYDRIEQT 144
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv0453	PF03544	33	0.002	Gram-negative bacterial tonB protein
>PF03544#Gram-negative bacterial tonB protein Length = 243 Score = 33.0 bits (75), Expect = 0.002 Identities = 20/72 (27%), Positives = 27/72 (37%) Query: 320 VPQPPVVPAPAPDAVVPTVLPLAGTATPTTAPASAPAAGAAPGPPAGTATATSASVPTSA 379 + +P P P P V P + PAS A P + TATA ++ TS Sbjct: 94 IEKPKPKPKPKPKPVKKVEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSV 153 Query: 380 GGFPPYLVGSGP 391 P L + P Sbjct: 154 ASGPRALSRNQP 165
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv0456A	TYPE3IMSPROT	26	0.021	Type III secretion system inner membrane S protein ...
>TYPE3IMSPROT#Type III secretion system inner membrane S protein family signature. Length = 354 Score = 26.3 bits (58), Expect = 0.021 Identities = 12/64 (18%), Positives = 24/64 (37%), Gaps = 13/64 (20%) Query: 2 LRGEIWQVDLDPARGSAA-NMRRPAVIVSNDRANAAAIRLDRGVVPVVPVTSNTEKVPIP 60 ++ + Q + + N++R +V+V+N A I R + P+P Sbjct: 234 IKSKRRQFHQEIQSRNMRENVKRSSVVVANPTHIAIGILYKR------------GETPLP 281 Query: 61 GVVA 64 V Sbjct: 282 LVTF 285

Rv0681

Rv0691c

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
Rv0681	3	19	-2.918113	Probable transcriptional regulatory protein
Rv0682	2	16	-2.991961	30S ribosomal protein S12 RpsL
Rv0683	2	11	-2.616721	30S ribosomal protein S7 RpsG
Rv0684	2	12	-2.230946	Probable elongation factor G FusA1 (EF-G)
Rv0685	0	10	-1.008505	Probable iron-regulated elongation factor TU Tuf
Rv0686	-1	12	-1.378298	Probable membrane protein
Rv0687	0	13	-1.138690	Probable short-chain type
Rv0688	0	13	-1.070126	Putative ferredoxin reductase
Rv0689c	-3	14	-0.703927	Hypothetical protein
Rv0690c	-2	13	-1.127676	Conserved hypothetical protein
Rv0691c	-2	13	-1.023353	Probable transcriptional regulatory protein

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv0681	TETREPRESSOR	50	7e-10	Tetracycline repressor protein signature.
>TETREPRESSOR#Tetracycline repressor protein signature. Length = 218 Score = 49.9 bits (119), Expect = 7e-10 Identities = 41/176 (23%), Positives = 71/176 (40%), Gaps = 25/176 (14%) Query: 5 AKLSRESIVEGALTFLDREGWDSLTINALATQLGTKGPSLYNHVDSLEDLRRAVRIRVID 64 A+L+RES+++ AL L+ G D LT LA +LG + P+LY HV + +RA+ + Sbjct: 2 ARLNRESVIDAALELLNETGIDGLTTRKLAQKLGIEQPTLYWHVKN----KRALLDALAV 57 Query: 65 DIITMLNRVGAGRARDDAVLVMAGAYRSY-----AHHHPGRYSAFTRMPLGGDDPEYTAA 119 +I+ + A + + S+ + + TR D+ +Y Sbjct: 58 EILARHHDYSLPAAGESWQSFLRNNAMSFRRALLRYRDGAKVHLGTR----PDEKQYDTV 113 Query: 120 TRGAAAPVIAVLSSYGLDGEQAFYAALEFWSALHGFVLLEMTGVMDDIDTDAVFTD 175 + ++ G YA SA+ F L V++ + A TD Sbjct: 114 ET-----QLRFMTENGFSLRDGLYAI----SAVSHFTLGA---VLEQQEHTAALTD 157
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv0684	TCRTETOQM	585	0.0	Tetracycline resistance protein TetO/TetQ/TetM family ...
>TCRTETOQM#Tetracycline resistance protein TetO/TetQ/TetM family signature. Length = 639 Score = 585 bits (1510), Expect = 0.0 Identities = 162/678 (23%), Positives = 305/678 (44%), Gaps = 71/678 (10%) Query: 12 RVRNFGIMAHIDAGKTTTTERILYYTGINYKIGEVHDGAATMDWMEQEQERGITITSAAT 71 ++ N G++AH+DAGKTT TE +LY +G ++G V G D E++RGITI + T Sbjct: 2 KIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTDNTLLERQRGITIQTGIT 61 Query: 72 TTFWKDNQLNIIDTPGHVDFTVEVERNLRVLDGAVAVFDGKEGVEPQSEQVWRQADKYDV 131 + W++ ++NIIDTPGH+DF EV R+L VLDGA+ + K+GV+ Q+ ++ K + Sbjct: 62 SFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAKDGVQAQTRILFHALRKMGI 121 Query: 132 PRICFVNKMDKIGADFYFSVRTMGERLGANAVPIQLPVGAEADFEGVVDLVEMNAKVWRG 191 P I F+NK+D+ G D + + E+L A V Q Sbjct: 122 PTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQ------------------------- 156 Query: 192 ETKLGETYDTVEIPADLAEQAEEYRTKLLEVVAESDEHLLEKYLGGEELTVDEIKGAIRK 251 + + ++E++ + V E ++ LLEKY+ G+ L E++ Sbjct: 157 -----KVELYPNMCVTNFTESEQW-----DTVIEGNDDLLEKYMSGKSLEALELEQEESI 206 Query: 252 LTIASEIYPVLCGSAFKNKGVQPMLDAVVDYLPSPLDVPPAIGHAPAKEDEEVVRKATTD 311 ++PV GSA N G+ +++ + + S Sbjct: 207 RFHNCSLFPVYHGSAKNNIGIDNLIEVITNKFYSSTH--------------------RGQ 246 Query: 312 EPFAALAFKIATHPFFGKLTYIRVYSGTVESGSQVINATKGKKERLGKLFQMHSNKENPV 371 FKI +L YIR+YSG + V + K K ++ +++ + + + Sbjct: 247 SELCGKVFKIEYSEKRQRLAYIRLYSGVLHLRDSVRISEKEKI-KITEMYTSINGELCKI 305 Query: 372 DRASAGHIYAVIG----LKDTTTGDTLSDPNQQIVLESMTFPDPVIEVAIEPKTKSDQEK 427 D+A +G I + L GDT P + E + P P+++ +EP +E Sbjct: 306 DKAYSGEIVILQNEFLKLNS-VLGDTKLLPQR----ERIENPLPLLQTTVEPSKPQQREM 360 Query: 428 LSLSIQKLAEEDPTFKVHLDSETGQTVIGGMGELHLDILVDRMRREFKVEANVGKPQVAY 487 L ++ ++++ DP + ++DS T + ++ +G++ +++ ++ ++ VE + +P V Y Sbjct: 361 LLDALLEISDSDPLLRYYVDSATHEIILSFLGKVQMEVTCALLQEKYHVEIEIKEPTVIY 420 Query: 488 KETIKRLVQNVEYTHKKQTGGSGQFAKVIINLEPFTGEEGATYEFESKVTGGRIPREYIP 547 E + EYT + + +A + +++ P G+ ++ES V+ G + + + Sbjct: 421 MERPLK---KAEYTIHIEVPPNPFWASIGLSVSP--LPLGSGMQYESSVSLGYLNQSFQN 475 Query: 548 SVDAGAQDAMQYGVLAGYPLVNLKVTLLDGAYHEVDSSEMAFKIAGSQVLKKAAALAQPV 607 +V G + + G L G+ + + K+ G Y+ S+ F++ VL++ A Sbjct: 476 AVMEGIRYGCEQG-LYGWNVTDCKICFKYGLYYSPVSTPADFRMLAPIVLEQVLKKAGTE 534 Query: 608 ILEPIMAVEVTTPEDYMGDVIGDLNSRRGQIQAMEERAGARVVRAHVPLSEMFGYVGDLR 667 +LEP ++ ++ P++Y+ D I + + ++ +P + Y DL Sbjct: 535 LLEPYLSFKIYAPQEYLSRAYTDAPKYCANIVDTQLKNNEVILSGEIPARCIQEYRSDLT 594 Query: 668 SKTQGRANYSMVFDSYSE 685 T GR+ Y Sbjct: 595 FFTNGRSVCLTELKGYHV 612
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv0685	TCRTETOQM	80	3e-18	Tetracycline resistance protein TetO/TetQ/TetM family ...
>TCRTETOQM#Tetracycline resistance protein TetO/TetQ/TetM family signature. Length = 639 Score = 79.9 bits (197), Expect = 3e-18 Identities = 53/153 (34%), Positives = 81/153 (52%), Gaps = 9/153 (5%) Query: 13 VNIGTIGHVDHGKTTLTAAITKVLHDK--FPDLNETKAFD-QIDNAPEERQRGITINIAH 69 +NIG + HVD GKTTLT ++ L++ +L + DN ERQRGITI Sbjct: 4 INIGVLAHVDAGKTTLTESL---LYNSGAITELGSVDKGTTRTDNTLLERQRGITIQTGI 60 Query: 70 VEYQTDKRHYAHVDAPGHADYIKNMITGAAQMDGAILVVAATDGPMPQTREHVLLARQVG 129 +Q + +D PGH D++ + + +DGAIL+++A DG QTR R++G Sbjct: 61 TSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAKDGVQAQTRILFHALRKMG 120 Query: 130 VPYILVALNKADAVDDEELLELVEMEVRELLAA 162 +P I +NK D + L V +++E L+A Sbjct: 121 IPTI-FFINKIDQNGID--LSTVYQDIKEKLSA 150
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv0687	DHBDHDRGNASE	124	3e-36	2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase signature. Length = 261 Score = 124 bits (311), Expect = 3e-36 Identities = 93/278 (33%), Positives = 138/278 (49%), Gaps = 27/278 (9%) Query: 1 MSARGGSLHGRVAFVTGAARAQGRSHAVRLAREGADIVALDICAPVSGSVTYPPATSEDL 60 M+A+G + G++AF+TGAA+ G + A LA +GA I A+D P E L Sbjct: 1 MNAKG--IEGKIAFITGAAQGIGEAVARTLASQGAHIAAVD-YNP------------EKL 45 Query: 61 GETVRAVEAEGRKVLAREVDIRDDAELRRLVADGVEQFGRLDIVVANAGVLGWGRLWELT 120 + V +++AE R A D+RD A + + A + G +DI+V AGVL G + L+ Sbjct: 46 EKVVSSLKAEARHAEAFPADVRDSAAIDEITARIEREMGPIDILVNVAGVLRPGLIHSLS 105 Query: 121 DEQWETVIGVNLTGTWRTLRATVPAMIDAGNGGSIVVVSSSAGLKATPGNGHYAASKHAL 180 DE+WE VN TG + R+ M+D GSIV V S+ YA+SK A Sbjct: 106 DEEWEATFSVNSTGVFNASRSVSKYMMDR-RSGSIVTVGSNPAGVPRTSMAAYASSKAAA 164 Query: 181 VALTNTLAIELGEFGIRVNSIHPYSVDTPM-----IEPEAMIQTFAKHPGYVHSFP-PMP 234 V T L +EL E+ IR N + P S +T M + Q G + +F +P Sbjct: 165 VMFTKCLGLELAEYNIRCNIVSPGSTETDMQWSLWADENGAEQVIK---GSLETFKTGIP 221 Query: 235 LQPKGFMTPDEISDVVVWLAGDGSGALSGNQIPVDKGA 272 L K P +I+D V++L +G ++ + + VD GA Sbjct: 222 L--KKLAKPSDIADAVLFLVSGQAGHITMHNLCVDGGA 257
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv0691c	HTHTETR	117	2e-35	TetR bacterial regulatory protein HTH signature.
>HTHTETR#TetR bacterial regulatory protein HTH signature. Length = 215 Score = 117 bits (295), Expect = 2e-35 Identities = 35/205 (17%), Positives = 71/205 (34%), Gaps = 15/205 (7%) Query: 4 ESRVGRRRSTTPHHISDVAIELFAAHGFTDVSVDDIARAAGIARRTLFRYYASKNAIPWG 63 + + T HI DVA+ LF+ G + S+ +IA+AAG+ R ++ ++ K+ + Sbjct: 2 ARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSE 61 Query: 64 DFSTHLAQLQGLLDNIDSRI--QLRDALRAALLAFNTFDESETIRHRKRMRVILQTPELQ 121 + + + L ++ LR L+ + + T R+ + I+ Sbjct: 62 IWELSESNIGELELEYQAKFPGDPLSVLREILIHV--LESTVTEERRRLLMEIIFHKCEF 119 Query: 122 AYSMTMYAGWR-----EVIAKFV-----ARRSGGKTTDFMPQTVAWTMLGVALSAYEHW- 170 M + + E + + D M + A M G E+W Sbjct: 120 VGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMTRRAAIIMRGYISGLMENWL 179 Query: 171 LRDESVSLTEALGAAFDVVGAGLDR 195 +S L + ++ Sbjct: 180 FAPQSFDLKKEARDYVAILLEMYLL 204

Rv0754

Rv0758

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
Rv0754	3	12	-1.345947	PE-PGRS family protein PE_PGRS11
Rv0755c	3	14	-2.758797	PPE family protein PPE12
Rv0755A	-2	14	-0.704488	Putative transposase (fragment)
Rv0756c	-1	11	-0.686081	*Unknown protein
Rv0757	-1	11	-1.606801	Possible two component system response
Rv0758	-1	12	-1.283263	Possible two component system response sensor

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv0754	cloacin	42	6e-06	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 42.0 bits (98), Expect = 6e-06 Identities = 34/110 (30%), Positives = 42/110 (38%), Gaps = 6/110 (5%) Query: 175 GDGGAGYTGGNGGSAGLIGNGGTGGAGFAGGVGGMGGTGGWLMGNGGMGGAG-GVGGNGG 233 G G G+ G ++G I NGG G G GG G GG G+G GG G Sbjct: 3 GGDGRGHNTGAHSTSGNI-NGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 234 AGGQALLFGNGGLGGAGGAGGVDGAIGRGGWFIGTGGMATIGGGGNGQSI 283 G G G G G G + + G ++T G GG SI Sbjct: 62 HGNG----GGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSI 107 Score = 41.6 bits (97), Expect = 8e-06 Identities = 36/98 (36%), Positives = 40/98 (40%), Gaps = 8/98 (8%) Query: 157 GVTGTAAAPNGGPGGLLFGDGGAGYTGGNGGSAGLIGNGGTG-GAGFAGGVGGMGGTGGW 215 G T+ NGGP GL G GG G S GG+G G + GG G G Sbjct: 12 GAHSTSGNINGGPTGL--GVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGG--- 66 Query: 216 LMGNGGMGGAGGVGGNGGAGGQALLFGNGGLGGAGGAG 253 GNG GG G GGN A + FG L G G Sbjct: 67 --GNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG 102 Score = 37.4 bits (86), Expect = 1e-04 Identities = 39/137 (28%), Positives = 56/137 (40%), Gaps = 13/137 (9%) Query: 197 TGGAGFAGGVGGMGGTGGWLMGNGGMGGAGGVGGNGGAGGQALLFGNGGLGGAGGAGGVD 256 +GG G G G T G + G G GG G + G+G + GG G+G G Sbjct: 2 SGGDG-RGHNTGAHSTSGNINGGPTGLGVGG-GASDGSGWSSENNPWGGGSGSGIHWGGG 59 Query: 257 GAIGRGGWFIGTGGMATIGGGGNGQSIVIDF-VRHGQTPGNAAMLIDTAVPGPGLTALGQ 315 G GG +GG + GG + + + F TPG G ++ Sbjct: 60 SGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAG---------GLAVSISAG 110 Query: 316 QQAQAIANALAA-KGPY 331 + AIA+ +AA KGP+ Sbjct: 111 ALSAAIADIMAALKGPF 127
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv0755c	cloacin	35	0.001	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 35.1 bits (80), Expect = 0.001 Identities = 28/93 (30%), Positives = 32/93 (34%), Gaps = 2/93 (2%) Query: 253 SGNIGNANVGGGNSGDNNFGFGNFGNANIGIGNAGPNMSSPAVPTPGNGNVGIGNGGNGN 312 SG G + G +S N G G G + G SS P G GI GG Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGS- 60 Query: 313 FGGGNTGNANIGLGNVGDGNVGFGNSGSYNFGF 345 G GN G G G G + FGF Sbjct: 61 -GHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGF 92 Score = 32.8 bits (74), Expect = 0.004 Identities = 27/75 (36%), Positives = 36/75 (48%), Gaps = 1/75 (1%) Query: 310 NGNFGGGNTGNANIGLGNVGDGNVGFGNSGSYNFGFGNTGNNNIGIGLTGSNQIGFGGLN 369 +G G G+ A+ GN+ G G G G + G G + NN G +GS GG Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 370 SGSGNIGFGNSGTGN 384 G+G G GNSG G+ Sbjct: 62 HGNGG-GNGNSGGGS 75 Score = 30.1 bits (67), Expect = 0.033 Identities = 27/92 (29%), Positives = 37/92 (40%), Gaps = 15/92 (16%) Query: 226 GSGNIGNFNLGSGNGNVGIGPSSFNVGSGNIGNANVGGGNSGDNNFGFGNFGNANIGIGN 285 G G N S +GN+ GP+ VG GG + G G+ + N G Sbjct: 4 GDGRGHNTGAHSTSGNINGGPTGLGVG---------GGASDGS---GWSSENNPWGGGSG 51 Query: 286 AGPNMSSPAVPTPGNGNVGIGNGGNGNFGGGN 317 +G + + G GN GN G G+ GGN Sbjct: 52 SGIHWGGGSGHGNGGGN---GNSGGGSGTGGN 80
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv0757	HTHFIS	106	2e-28	FIS bacterial regulatory protein HTH signature.
>HTHFIS#FIS bacterial regulatory protein HTH signature. Length = 484 Score = 106 bits (265), Expect = 2e-28 Identities = 36/136 (26%), Positives = 63/136 (46%) Query: 21 ARVLVVDDEANIVELLSVSLKFQGFEVYTATNGAQALDRARETRPDAVILDVMMPGMDGF 80 A +LV DD+A I +L+ +L G++V +N A D V+ DV+MP + F Sbjct: 4 ATILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAF 63 Query: 81 GVLRRLRADGIDAPALFLTARDSLQDKIAGLTLGGDDYVTKPFSLEEVVARLRVILRRAG 140 +L R++ D P L ++A+++ I G DY+ KPF L E++ + L Sbjct: 64 DLLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAEPK 123 Query: 141 KGNKEPRNVRLTFADI 156 + + + + Sbjct: 124 RRPSKLEDDSQDGMPL 139
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv0758	PF06580	37	1e-04	Sensor histidine kinase
>PF06580#Sensor histidine kinase Length = 349 Score = 36.8 bits (85), Expect = 1e-04 Identities = 20/109 (18%), Positives = 37/109 (33%), Gaps = 26/109 (23%) Query: 366 VLRNLVANAIQH----TPESADVTVRVGTEGDDAILEVADDGPGMSQEDALRVFERFYRA 421 +++ LV N I+H P+ + ++ + LEV + G + Sbjct: 259 LVQTLVENGIKHGIAQLPQGGKILLKGTKDNGTVTLEVENTGSLALKNT----------- 307 Query: 422 DSSRARASGGTGLGLSIVDS-LVAAHGG--AVTVTTALGEGCCFRVSLP 467 TG GL V L +G + ++ G+ V +P Sbjct: 308 -------KESTGTGLQNVRERLQMLYGTEAQIKLSEKQGKVNA-MVLIP 348

Rv0872c

Rv0879c

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
Rv0872c	0	7	2.181736	PE-PGRS family protein PE_PGRS15
Rv0873	-1	9	-0.207278	Probable acyl-CoA dehydrogenase FadE10
Rv0874c	-1	11	0.804647	Conserved hypothetical protein
Rv0875c	2	12	-0.056258	Possible conserved exported protein
Rv0876c	0	14	0.114460	Possible conserved transmembrane protein
Rv0877	1	16	0.468091	Conserved hypothetical protein
Rv0878c	2	15	0.668359	PPE family protein PPE13
Rv0879c	-1	12	1.195848	Possible conserved transmembrane protein

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv0872c	cloacin	37	1e-04	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 37.4 bits (86), Expect = 1e-04 Identities = 30/85 (35%), Positives = 40/85 (47%) Query: 427 AGGDGGAANTDSAGSSRKAFGGDGGVGGDGASALGTGGEGGIGGQGGNGGAGGLLIGNGG 486 +GGDG NT + +S GG G+G G ++ G+G GG G+G G G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 487 AGGVGGTAGAGGTGGSGGAGGAGGA 511 G GG +GG G+GG A A Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAA 86 Score = 36.6 bits (84), Expect = 3e-04 Identities = 30/88 (34%), Positives = 34/88 (38%), Gaps = 4/88 (4%) Query: 486 GAGGVGGTAGAGGTGGSGGAGGAGGAGGGGTNSGPGAAFGGNGNTGGNGGNGGAPGALGG 545 G G G GA T G+ G G GGG + G G + N GG+G G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 546 KGGSGGLIGRAGSDGGVGAGGAGGAGGA 573 G G S GG G GG A A Sbjct: 63 GNGGGN----GNSGGGSGTGGNLSAVAA 86 Score = 36.2 bits (83), Expect = 4e-04 Identities = 32/86 (37%), Positives = 37/86 (43%), Gaps = 2/86 (2%) Query: 461 GTGGEGGIGGQGGN--GGAGGLLIGNGGAGGVGGTAGAGGTGGSGGAGGAGGAGGGGTNS 518 G G G GN GG GL +G G + G G ++ GG G+G G G G N Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNG 65 Query: 519 GPGAAFGGNGNTGGNGGNGGAPGALG 544 G GG TGGN AP A G Sbjct: 66 GGNGNSGGGSGTGGNLSAVAAPVAFG 91 Score = 33.5 bits (76), Expect = 0.003 Identities = 26/67 (38%), Positives = 28/67 (41%) Query: 368 NGGSGGNGFDSFASGGTGGAGGTGGAGGRGGLLIGDGGAGGAGGVGGTGGSGAPGGGGGA 427 NGG G G AS G+G + GG G I GG G G GG G SG G GG Sbjct: 21 NGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNGNSGGGSGTGGN 80 Query: 428 GGDGGAA 434 A Sbjct: 81 LSAVAAP 87 Score = 33.1 bits (75), Expect = 0.003 Identities = 29/96 (30%), Positives = 36/96 (37%), Gaps = 9/96 (9%) Query: 484 NGGAGGVGGTAGAGGTGGSGGAGGAGGAG--------GGGTNSGPGAAFGGNGNTGGNGG 535 N GA G G TG G G + G+G GGG+ SG GG+G+ G G Sbjct: 10 NTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHW-GGGSGHGNGGGN 68 Query: 536 NGGAPGALGGKGGSGGLIGRAGSDGGVGAGGAGGAG 571 G+ G S A + GAGG Sbjct: 69 GNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 32.4 bits (73), Expect = 0.006 Identities = 29/94 (30%), Positives = 35/94 (37%), Gaps = 1/94 (1%) Query: 510 GAGGGGTNSGPGAAFGG-NGNTGGNGGNGGAPGALGGKGGSGGLIGRAGSDGGVGAGGAG 568 G G G N+G + G NG G G GGA G + G +GS G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 569 GAGGAGGTGGEGGTGGDGKTTDGNPGMGGSPGSA 602 G GG G G G G + P G P + Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALS 96 Score = 31.6 bits (71), Expect = 0.009 Identities = 25/76 (32%), Positives = 26/76 (34%) Query: 404 GGAGGAGGVGGTGGSGAPGGGGGAGGDGGAANTDSAGSSRKAFGGDGGVGGDGASALGTG 463 GG G G SG GG G GG A+ S SS G G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 464 GEGGIGGQGGNGGAGG 479 G GG G G G G Sbjct: 63 GNGGGNGNSGGGSGTG 78 Score = 31.6 bits (71), Expect = 0.011 Identities = 37/119 (31%), Positives = 47/119 (39%), Gaps = 9/119 (7%) Query: 146 GSGGVGQAGGAGGSAGLIGIGGTGGAGGAGAVGGVGGNGGWLYGNGGAGGLGGTGVAGVN 205 G G G GA ++G I GG G G GG GW N GG G+G+ Sbjct: 3 GGDGRGHNTGAHSTSGNI----NGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGG 58 Query: 206 GGMGAAGGAGGNAYLFGSGGAGGQGGMGAAGADGVNPTPTGTADAGSTGTDQTLGGNAI 264 G GG GN SGG G GG +A A V + G+ G ++ A+ Sbjct: 59 GSGHGNGGGNGN-----SGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGAL 112 Score = 30.8 bits (69), Expect = 0.019 Identities = 27/91 (29%), Positives = 36/91 (39%), Gaps = 9/91 (9%) Query: 250 AGSTGTDQTLGGNAIGGNGGPGDAGDAMTSGGAGGSGGNAVSTVNGDAVGGEGGKGGEGA 309 +G G G ++ GN G G + G + GSG ++ + G G GG Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 310 YGGAGGAGGSAASIGNAAIGGNGGAGGNAQA 340 +G GG G S GG G GGN A Sbjct: 62 HGNGGGNGNS---------GGGSGTGGNLSA 83
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv0876c	RTXTOXINA	30	0.034	Gram-negative bacterial RTX toxin determinant A family...
>RTXTOXINA#Gram-negative bacterial RTX toxin determinant A family signature. Length = 1024 Score = 29.9 bits (67), Expect = 0.034 Identities = 17/48 (35%), Positives = 25/48 (52%), Gaps = 4/48 (8%) Query: 416 TVLVTVLA-IAAAVAGSLAATAIATL---ITAGSSAIAKASLDASLQH 459 TVL +V + I+AA SL ++ L +T S I +AS A +H Sbjct: 373 TVLASVSSGISAAATTSLVGAPVSALVGAVTGIISGILEASKQAMFEH 420
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv0878c	cloacin	35	4e-04	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 35.5 bits (81), Expect = 4e-04 Identities = 29/103 (28%), Positives = 39/103 (37%), Gaps = 25/103 (24%) Query: 263 GNGNDGNTNFGSGNAGFLNIGSGNEGSGNLGFGNAGDDNTGWGNSGDTNTGGFNSGDLNT 322 G G++ + SGN N G LG G D +GW + + GG SG Sbjct: 6 GRGHNTGAHSTSGNI--------NGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGI--- 54 Query: 323 GIGSPVTQGVANSGFGNTGTGHSGFFNSGNSGSGFQNLGNGSS 365 G +G G+ G +GNSG G GN S+ Sbjct: 55 ------------HWGGGSGHGNGG--GNGNSGGGSGTGGNLSA 83 Score = 30.1 bits (67), Expect = 0.022 Identities = 27/75 (36%), Positives = 33/75 (44%), Gaps = 1/75 (1%) Query: 243 GNGNIGNANLGSGNAGFFNFGNGNDGNTNFGSGNAGFLNIGSGNEGSGNLGFGNAGDDNT 302 G G+ A+ SGN G G G + GSG + N G GSG G +G N Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNG 65 Query: 303 GW-GNSGDTNTGGFN 316 G GNSG + G N Sbjct: 66 GGNGNSGGGSGTGGN 80
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv0879c	PF06580	29	0.002	Sensor histidine kinase
>PF06580#Sensor histidine kinase Length = 349 Score = 28.7 bits (64), Expect = 0.002 Identities = 14/67 (20%), Positives = 26/67 (38%), Gaps = 1/67 (1%) Query: 2 SVENSQIREPPPLPPVLLEVWPVIAVGALAWLVAAVAAFVVPGLASWRPVTVA-GLATGL 60 S Q + ++L V P V + W VA + + + + +PV LA + Sbjct: 61 SFIKRQGWLKLNMGQIILRVLPACVVIGMVWFVANTSIWRLLAFINTKPVAFTLPLALSI 120 Query: 61 LGTTIFV 67 + + V Sbjct: 121 IFNVVVV 127

Rv0977

Rv0985c

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
Rv0977	6	16	4.980558	PE-PGRS family protein PE_PGRS16
Rv0978c	2	13	2.246956	PE-PGRS family protein PE_PGRS17
Rv0979c	0	9	1.350047	Hypothetical protein
Rv0979A	0	9	1.261528	50S ribosomal protein L32 RpmF
Rv0980c	0	10	0.752781	PE-PGRS family protein PE_PGRS18
Rv0981	-2	12	-2.279486	Mycobacterial persistence regulator MRPA (two
Rv0982	1	24	-5.844563	Two component sensor kinase MprB
Rv0983	3	33	-8.076687	Probable serine protease PepD (serine
Rv0984	1	35	-8.957863	Possible pterin-4-alpha-carbinolamine
Rv0985c	2	35	-8.565029	Possible large-conductance ion mechanosensitive

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv0977	cloacin	44	2e-06	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 44.3 bits (104), Expect = 2e-06 Identities = 35/101 (34%), Positives = 43/101 (42%), Gaps = 1/101 (0%) Query: 468 AGGDGGQGDIGFDGGRGG-DGGPGGGGGAGGDGSGTFNAQANNGGDGGAGGVGGAGGTGG 526 +GGDG + G G +GGP G G GG G+ + NN GG+G GG G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 527 TGGVGADGGRGGDSGRGGDGGNAGHGGAAQFSGRGAYGGEG 567 G G +G GG SG GG+ A F G G Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG 102 Score = 43.9 bits (103), Expect = 3e-06 Identities = 37/108 (34%), Positives = 46/108 (42%), Gaps = 7/108 (6%) Query: 357 GGAGAGGDGGAGGIGGTGGNGSIGGAAGNGGNGGRGGA------GGMATAGSDGGNGGGG 410 GG G G + GA G G G G G + G G + GG + +G G G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 411 GNGGVGVGSAGGAGGTGGDGGAAGAGGAPGHGYFQQPAPQGLPIGTGG 458 GNGG G++GG GTGG+ A A A G P GL + Sbjct: 63 GNGGGN-GNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISA 109 Score = 42.0 bits (98), Expect = 9e-06 Identities = 30/82 (36%), Positives = 36/82 (43%), Gaps = 2/82 (2%) Query: 304 GIGEQGGQGGDGGA--GGAGGIGGSAGGIGGSQGAGGHGGDGGQGGAGGSGGVGGGGAGA 361 G G G G GG G+G G GS + + GG G+G G G G Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNG 65 Query: 362 GGDGGAGGIGGTGGNGSIGGAA 383 GG+G +GG GTGGN S A Sbjct: 66 GGNGNSGGGSGTGGNLSAVAAP 87 Score = 41.2 bits (96), Expect = 2e-05 Identities = 33/99 (33%), Positives = 42/99 (42%), Gaps = 1/99 (1%) Query: 158 GRGGDAGLFGHGGHGGVGGPGIAGAAGTAGLPGGNGANGGSGGIGGAGGAGGNGGLLFGN 217 GRG + G G+ G G+ G + G + N GG G+G G G Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNG 65 Query: 218 GGAGGQGGSGGLGGSGGTGGAGMAAG-PAGGTGGIGGIG 255 GG G GG G GG+ A +A G PA T G GG+ Sbjct: 66 GGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 41.2 bits (96), Expect = 2e-05 Identities = 32/79 (40%), Positives = 36/79 (45%), Gaps = 1/79 (1%) Query: 312 GGDGGAGGAGGIGGSAGGIGGSQGAGGHGGDGGQGGAGGSGGVGGGGAGAG-GDGGAGGI 370 GGDG G S GG G G GG G GGG+G+G GG G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 371 GGTGGNGSIGGAAGNGGNG 389 G GGNG+ GG +G GGN Sbjct: 63 GNGGGNGNSGGGSGTGGNL 81 Score = 40.5 bits (94), Expect = 3e-05 Identities = 30/104 (28%), Positives = 33/104 (31%) Query: 330 IGGSQGAGGHGGDGGQGGAGGSGGVGGGGAGAGGDGGAGGIGGTGGNGSIGGAAGNGGNG 389 + G G G + G G G G G G DG G G GG Sbjct: 1 MSGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGS 60 Query: 390 GRGGAGGMATAGSDGGNGGGGGNGGVGVGSAGGAGGTGGDGGAA 433 G G GG +G G GG V A T G GG A Sbjct: 61 GHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 40.1 bits (93), Expect = 4e-05 Identities = 27/80 (33%), Positives = 34/80 (42%) Query: 538 GDSGRGGDGGNAGHGGAAQFSGRGAYGGEGGSGGAGGNAGGAGTGGTAGSGGAGGFGGNG 597 G GRG + G G G G G S G+G ++ GG +GSG G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 598 ADGGNGGNGGNGGFGGINGT 617 +GG GN G G G N + Sbjct: 63 GNGGGNGNSGGGSGTGGNLS 82 Score = 39.3 bits (91), Expect = 7e-05 Identities = 36/104 (34%), Positives = 45/104 (43%), Gaps = 3/104 (2%) Query: 257 IGGAGGVGGHGSALFGHGGINGDGGTGGMGGQGGAGGNGWAAEGITVGIGEQGGQGGDGG 316 + G G G + A G ING G TG G G + G+GW++E G G G GG Sbjct: 1 MSGGDGRGHNTGAHSTSGNING-GPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGG 59 Query: 317 AGGAGGIGGSAGGIGGSQGAGGHGGDGGQGGAGGSGGVGGGGAG 360 +G GG G GG G GG+ A G + GAG Sbjct: 60 SGHGN--GGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAG 101 Score = 37.4 bits (86), Expect = 3e-04 Identities = 27/90 (30%), Positives = 35/90 (38%) Query: 558 SGRGAYGGEGGSGGAGGNAGGAGTGGTAGSGGAGGFGGNGADGGNGGNGGNGGFGGINGT 617 SG G G+ GN G TG G G + G G + + GG G+G G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 618 FGTNGAGGTGGLGTLLGGHNGNIGLNGATG 647 G G G G G+ GG+ + A G Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAAPVAFG 91 Score = 36.6 bits (84), Expect = 5e-04 Identities = 35/113 (30%), Positives = 45/113 (39%), Gaps = 14/113 (12%) Query: 492 GGGAGGDGSGTFNAQANNGGDGGAGGVGGAGGTGGTGGVGADGGRGGDSGRG-GDGGNAG 550 GG G +G + N G GVGG G + G+G + GG SG G GG +G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGG-GASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 551 HGGAAQFSGRGAYGGEGGSGGAGGNAGGAGTGGTAGSGGAGGFGGNGADGGNG 603 HG GG+G +GG +G G + A GF G G Sbjct: 62 HGNG------------GGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG 102 Score = 35.8 bits (82), Expect = 9e-04 Identities = 28/83 (33%), Positives = 34/83 (40%), Gaps = 1/83 (1%) Query: 195 NGGSGGIGGAGGAGGNGGLLFGNGGAGGQGGSG-GLGGSGGTGGAGMAAGPAGGTGGIGG 253 NGG G+G GGA G N GG GSG GG G G G GG+G G Sbjct: 21 NGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNGNSGGGSGTGGN 80 Query: 254 IGGIGGAGGVGGHGSALFGHGGI 276 + + G + G GG+ Sbjct: 81 LSAVAAPVAFGFPALSTPGAGGL 103 Score = 35.5 bits (81), Expect = 0.001 Identities = 31/80 (38%), Positives = 35/80 (43%), Gaps = 1/80 (1%) Query: 183 AGTAGLPGGNGANGGSGGI-GGAGGAGGNGGLLFGNGGAGGQGGSGGLGGSGGTGGAGMA 241 +G G GA+ SG I GG G G GG G+G + GG GSG G G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 242 AGPAGGTGGIGGIGGIGGAG 261 G GG G GG G GG Sbjct: 62 HGNGGGNGNSGGGSGTGGNL 81 Score = 35.5 bits (81), Expect = 0.001 Identities = 29/81 (35%), Positives = 33/81 (40%), Gaps = 6/81 (7%) Query: 422 GAGGTGGDGGAAGAGGAPGHGYFQQPAPQGLPIGTGGTGGEGGAGGAGGDGGQGDIGFDG 481 G G G + GA G G P GL +G G + G G + GG G Sbjct: 3 GGDGRGHNTGAHSTSGNINGG------PTGLGVGGGASDGSGWSSENNPWGGGSGSGIHW 56 Query: 482 GRGGDGGPGGGGGAGGDGSGT 502 G G G GGG G G GSGT Sbjct: 57 GGGSGHGNGGGNGNSGGGSGT 77 Score = 33.5 bits (76), Expect = 0.004 Identities = 23/77 (29%), Positives = 27/77 (35%) Query: 455 GTGGTGGEGGAGGAGGDGGQGDIGFDGGRGGDGGPGGGGGAGGDGSGTFNAQANNGGDGG 514 G G G GA G+ G G G G G G G G+ + GG G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 515 AGGVGGAGGTGGTGGVG 531 G G GG+G G Sbjct: 63 GNGGGNGNSGGGSGTGG 79 Score = 32.4 bits (73), Expect = 0.009 Identities = 26/81 (32%), Positives = 30/81 (37%), Gaps = 1/81 (1%) Query: 201 IGGAGGAGGNGGLLFGNGGA-GGQGGSGGLGGSGGTGGAGMAAGPAGGTGGIGGIGGIGG 259 + G G G N G +G GG G G GG+ G P GG G G G G Sbjct: 1 MSGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGS 60 Query: 260 AGGVGGHGSALFGHGGINGDG 280 G GG G G G+ Sbjct: 61 GHGNGGGNGNSGGGSGTGGNL 81 Score = 30.8 bits (69), Expect = 0.025 Identities = 27/74 (36%), Positives = 32/74 (43%), Gaps = 11/74 (14%) Query: 134 NGGAGGILWGNGGNGGSGAPGQPGGRGGDAGLFGHGGHGGVGGPGIAGAAGTAGLPGGNG 193 NGG G+ G G + GSG + GG +G H G G G G GGNG Sbjct: 21 NGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNG-----------GGNG 69 Query: 194 ANGGSGGIGGAGGA 207 +GG G GG A Sbjct: 70 NSGGGSGTGGNLSA 83 Score = 30.5 bits (68), Expect = 0.037 Identities = 24/71 (33%), Positives = 28/71 (39%), Gaps = 7/71 (9%) Query: 379 IGGAAGNGGNGGRGGAGGMATAGSDGGNGGGGGNGGVGVGSA----GGAGGTG---GDGG 431 + G G G N G G G G GGG + G G S GG G+G G G Sbjct: 1 MSGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGS 60 Query: 432 AAGAGGAPGHG 442 G GG G+ Sbjct: 61 GHGNGGGNGNS 71
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv0978c	cloacin	37	8e-05	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 37.0 bits (85), Expect = 8e-05 Identities = 28/90 (31%), Positives = 35/90 (38%), Gaps = 5/90 (5%) Query: 122 NGIDGTGQAGGNGGWLWGNGGNGGSGAPGQAGGAGGAAGLIGNGGAGGTGGAVSLARAGT 181 +G DG G G + +G P G GGA+ G G S + Sbjct: 2 SGGDGRGHNTGAH-----STSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHW 56 Query: 182 AGGAGRGPVGGIGGAGGVGGAGGAAGAVTT 211 GG+G G GG G +GG G GG AV Sbjct: 57 GGGSGHGNGGGNGNSGGGSGTGGNLSAVAA 86 Score = 32.0 bits (72), Expect = 0.003 Identities = 24/78 (30%), Positives = 29/78 (37%), Gaps = 6/78 (7%) Query: 117 IGDGANGIDGTGQA------GGNGGWLWGNGGNGGSGAPGQAGGAGGAAGLIGNGGAGGT 170 +G G DG+G + GG G GG G G G G +GG +G GN A Sbjct: 27 LGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNGNSGGGSGTGGNLSAVAA 86 Query: 171 GGAVSLARAGTAGGAGRG 188 A T G G Sbjct: 87 PVAFGFPALSTPGAGGLA 104 Score = 31.6 bits (71), Expect = 0.005 Identities = 32/103 (31%), Positives = 36/103 (34%), Gaps = 4/103 (3%) Query: 117 IGDGANGIDGTGQAGGNGGWLWGNGGNGGSGAPGQAGGAGGAAGLIGNGGAGG----TGG 172 I G G+ G A GW N GG G G G G G G G TGG Sbjct: 20 INGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNGNSGGGSGTGG 79 Query: 173 AVSLARAGTAGGAGRGPVGGIGGAGGVGGAGGAAGAVTTITHA 215 +S A A G G GG AG + A+ I A Sbjct: 80 NLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAAIADIMAA 122
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv0980c	cloacin	42	4e-06	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 42.0 bits (98), Expect = 4e-06 Identities = 40/121 (33%), Positives = 49/121 (40%), Gaps = 1/121 (0%) Query: 170 AGGQGLPFEAGANGGAGGAGGWLFGNGGAGGVGGAGGAGTTFGVAGGDGGTGGVGGHGGL 229 +GG G GA+ +G G G G GG G + GG G+G G G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 230 IGVGGHGGDGGTG-GTGGAVSLARAGTAGGAGGGPAGGIGGAGGVGGAGGAAGAVTTITH 288 G GG G+ G G GTGG +S A A G G GG AG + A+ I Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAAIADIMA 121 Query: 289 A 289 A Sbjct: 122 A 122 Score = 39.3 bits (91), Expect = 3e-05 Identities = 26/78 (33%), Positives = 33/78 (42%), Gaps = 1/78 (1%) Query: 139 GNGGNGGSGAPGQAGGAGGAAGLIGNGGAGGAGGQGLPFEAGANGGAGGAGGWLFGNGGA 198 G+G +GA +G G +G GG G + G G E GG G+G G G Sbjct: 4 GDGRGHNTGAHSTSGNINGGPTGLGVGG-GASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 199 GGVGGAGGAGTTFGVAGG 216 G GG G +G G G Sbjct: 63 GNGGGNGNSGGGSGTGGN 80 Score = 33.9 bits (77), Expect = 0.001 Identities = 30/117 (25%), Positives = 35/117 (29%), Gaps = 11/117 (9%) Query: 118 GDGANGIDGTGQAGGNGGWLWGNGGNGGSGAPGQAGGAGGAAGLIGNGGAGGAGGQGLPF 177 GDG G GN NGG G GGA +G G G Sbjct: 4 GDGRGHNTGAHSTSGNI--------NGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIH 55 Query: 178 EAGANGGAGGAGGWLFGNGGAGGVGGAGGAGTTFGVAGGDGGTGGVGGHGGLIGVGG 234 G +G G G GN G G G + VA G G G + + Sbjct: 56 WGGGSGHGNGGGN---GNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISA 109 Score = 33.1 bits (75), Expect = 0.003 Identities = 29/86 (33%), Positives = 35/86 (40%), Gaps = 13/86 (15%) Query: 213 VAGGDGGTGGVGGH-------GGLIGVGGHGGDGGTGGTG------GAVSLARAGTAGGA 259 ++GGDG G H GG G+G GG G G S + GG+ Sbjct: 1 MSGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGS 60 Query: 260 GGGPAGGIGGAGGVGGAGGAAGAVTT 285 G G GG G +GG G GG AV Sbjct: 61 GHGNGGGNGNSGGGSGTGGNLSAVAA 86 Score = 29.3 bits (65), Expect = 0.033 Identities = 26/78 (33%), Positives = 33/78 (42%), Gaps = 5/78 (6%) Query: 117 IGDGANGIDGTGQAGGNGGWLWGNGGN---GGSGAPGQAGGAGGAAGLIGNGGAGGAGGQ 173 +G G DG+G + N W G+G GG G GG G + G G GG A Sbjct: 27 LGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNGNSGGGSGTGGNLSAVAA 86 Query: 174 GLPF--EAGANGGAGGAG 189 + F A + GAGG Sbjct: 87 PVAFGFPALSTPGAGGLA 104
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv0981	HTHFIS	103	9e-28	FIS bacterial regulatory protein HTH signature.
>HTHFIS#FIS bacterial regulatory protein HTH signature. Length = 484 Score = 103 bits (258), Expect = 9e-28 Identities = 45/135 (33%), Positives = 71/135 (52%), Gaps = 1/135 (0%) Query: 2 RILVVDDDRAVRESLRRSLSFNGYSVELAHDGVEALDMIASDRPDALVLDVMMPRLDGLE 61 ILV DDD A+R L ++LS GY V + + IA+ D +V DV+MP + + Sbjct: 5 TILVADDDAAIRTVLNQALSRAGYDVRITSNAATLWRWIAAGDGDLVVTDVVMPDENAFD 64 Query: 62 VCRQLRGTGDDLPILVLTARDSVSERVAGLDAGADDYLPKPFALEELLARM-RALLRRTK 120 + +++ DLP+LV++A+++ + + GA DYLPKPF L EL+ + RAL + Sbjct: 65 LLPRIKKARPDLPVLVMSAQNTFMTAIKASEKGAYDYLPKPFDLTELIGIIGRALAEPKR 124 Query: 121 PEDAAESMAMRFSDL 135 E + L Sbjct: 125 RPSKLEDDSQDGMPL 139
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv0983	V8PROTEASE	57	4e-11	V8 serine protease family signature.
>V8PROTEASE#V8 serine protease family signature. Length = 336 Score = 57.3 bits (138), Expect = 4e-11 Identities = 36/182 (19%), Positives = 72/182 (39%), Gaps = 27/182 (14%) Query: 165 PSVVMLETDLGRQSEEGSGIILSAEGLILTNNHVIAAAAKPPLG---SPPPKTTVTFSDG 221 V ++ + + SG+++ + +LTN HV+ A P P + +G Sbjct: 88 APVTYIQVEAPTGTFIASGVVV-GKDTLLTNKHVVDATHGDPHALKAFPSAINQDNYPNG 146 Query: 222 RTAPFTVVGADPTSDIAVVRV----QGVS---GLTPISLGSSSDLRVGQPVLAIGSPLGL 274 + D+A+V+ Q + P ++ ++++ +V Q + G P Sbjct: 147 GFTAEQITKYSGEGDLAIVKFSPNEQNKHIGEVVKPATMSNNAETQVNQNITVTGYPGDK 206 Query: 275 EGTVTTGIVSALNRPVSTTGEAGNQNTVLD--AIQTDAAINPGNSGGALVNMNAQLVGVN 332 PV+T E+ + T L A+Q D + GNSG + N +++G++ Sbjct: 207 --------------PVATMWESKGKITYLKGEAMQYDLSTTGGNSGSPVFNEKNEVIGIH 252 Query: 333 SA 334 Sbjct: 253 WG 254
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv0985c	MECHCHANNEL	162	1e-54	Bacterial mechano-sensitive ion channel signature.
>MECHCHANNEL#Bacterial mechano-sensitive ion channel signature. Length = 136 Score = 162 bits (412), Expect = 1e-54 Identities = 47/134 (35%), Positives = 66/134 (49%), Gaps = 6/134 (4%) Query: 1 MLKGFKEFLARGNIVDLAVAVVIGTAFTALVTKFTDSIITPLINR----IGVNAQSDVGI 56 ++K F+EF RGN+VDLAV V+IG AF +V+ II P + I + Sbjct: 3 IIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPPLGLLIGGIDFKQFAVTLR 62 Query: 57 LRIGIGGGQTIDLNVLLSAAINFFLIAFAVYFLVVLPYNTLRKKGE-VEQPGDT-QVVLL 114 G + V + +F ++AFA++ + L RKK E P T + VLL Sbjct: 63 DAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKLNRKKEEPAAAPAPTKEEVLL 122 Query: 115 TEIRDLLAQTNGDS 128 TEIRDLL + N S Sbjct: 123 TEIRDLLKEQNNRS 136

Rv1232c

Rv1238

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
Rv1232c	-3	14	-1.127060	Conserved protein
Rv1233c	-1	15	-2.616895	Conserved hypothetical membrane protein
Rv1234	-3	13	-3.219081	Probable transmembrane protein
Rv1235	-1	12	-2.528035	Probable sugar-binding lipoprotein LpqY
Rv1236	0	12	-3.167634	Probable sugar-transport integral membrane
Rv1237	1	11	-3.050139	Probable sugar-transport integral membrane
Rv1238	0	12	0.804890	Probable sugar-transport ATP-binding protein ABC

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv1232c	FLGMOTORFLIG	32	0.005	Flagellar motor switch protein FliG signature.
>FLGMOTORFLIG#Flagellar motor switch protein FliG signature. Length = 344 Score = 31.7 bits (72), Expect = 0.005 Identities = 21/130 (16%), Positives = 51/130 (39%), Gaps = 20/130 (15%) Query: 167 LAMPGQDVAQL---LDQFEGWKAVDVADAIRGLPPKRRHEVFKALHDKRLADVLQELPEL 223 +++ + +++ L Q E + + + + V + +A + + Sbjct: 26 VSIGSEISSKVFKYLSQEEIESLTFEIAKLETITSELKDNVLLEFKELMMAQEFIQKGGI 85 Query: 224 DQA-EVLSQ-LGTERAADV---------------LEEMDPDDAADLLAVLNPTEAELLLT 266 D A E+L + LGT++A D+ + DP + + + +P L+L+ Sbjct: 86 DYARELLEKSLGTQKAVDIINNLGSALQSRPFEFVRRADPANILNFIQQEHPQTIALILS 145 Query: 267 RMDPGDSGQV 276 +DP + + Sbjct: 146 YLDPQKASFI 155 Score = 30.2 bits (68), Expect = 0.015 Identities = 28/164 (17%), Positives = 56/164 (34%), Gaps = 30/164 (18%) Query: 181 FEGWKAVD---VADAIRGLPPKRRHEVFKALHDKRLADVLQELPELDQAEVLSQLGTERA 237 FE + D + + I+ P+ + L ++ + +L LP Q V ++ Sbjct: 117 FEFVRRADPANILNFIQQEHPQTIALILSYLDPQKASFILSSLPTEVQTNVARRIAL--- 173 Query: 238 ADVLEEMDPDDAADLLAVLNPTEAELLLTR-------------MDPGDSGQVRRLL---- 280 ++ P+ ++ VL A L ++ D + ++ Sbjct: 174 ---MDRTSPEVVREVERVLEKKLASLSSEDYTSAGGVDNVVEIINMADRKTEKFIIESLE 230 Query: 281 THSPDTAGG----LMTSDPVVLTPDTSIAEALARVRDPDLTPAL 320 P+ A + + +VL D SI L + +L AL Sbjct: 231 EEDPELAEEIKKKMFVFEDIVLLDDRSIQRVLREIDGQELAKAL 274
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv1233c	PF03544	30	0.004	Gram-negative bacterial tonB protein
>PF03544#Gram-negative bacterial tonB protein Length = 243 Score = 29.9 bits (67), Expect = 0.004 Identities = 13/85 (15%), Positives = 16/85 (18%) Query: 18 GPPPVGERPPEQPIADAPWAPPASSPMANHPPPAYPPSGYPPAYQPGYPTGYPPPMPPGG 77 P P E PE P P P +P P Sbjct: 75 EPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKPVKKVEQPKRDVKPVESRPASPFENTAP 134 Query: 78 YAPPGYPPPGTSSAGYGDIPYPPMP 102 P +S + P Sbjct: 135 ARPTSSTATAATSKPVTSVASGPRA 159
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv1235	MALTOSEBP	49	2e-08	Maltose binding protein signature.
>MALTOSEBP#Maltose binding protein signature. Length = 396 Score = 49.3 bits (117), Expect = 2e-08 Identities = 43/162 (26%), Positives = 70/162 (43%), Gaps = 16/162 (9%) Query: 139 WNHKLYAAPVTTNTQLLWYRPDLVNSPPTDWNAMIAEAARLHAAGEPSWIAVQANQGEGL 198 +N KL A P+ L Y DL+ +PP W + A L A G+ A+ N E Sbjct: 125 YNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKS---ALMFNLQEPY 181 Query: 199 VVWFNTLLVSAGGSVLS-EDGRHVTL---TDTPAHRAATVSALQILKSVATTPGADPSIT 254 W L+ + GG E+G++ D +A + ++K+ D SI Sbjct: 182 FTW--PLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSI- 238 Query: 255 RTEEGSARLAFEQGKAALEVNWPFVFASMLENAVKGGVPFLP 296 A AF +G+ A+ +N P+ ++++ + V GV LP Sbjct: 239 ------AEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLP 274
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv1238	PF05272	35	4e-04	Virulence-associated E family protein
>PF05272#Virulence-associated E family protein Length = 892 Score = 35.4 bits (81), Expect = 4e-04 Identities = 24/122 (19%), Positives = 39/122 (31%), Gaps = 35/122 (28%) Query: 33 LILVGPSGCGKTTTLNMIAGLEDISSGELRIAGERVNEKAPKDRDIAMVFQSYALYPHMT 92 ++L G G GK+T +N + GL+ S I +D Y Sbjct: 599 VVLEGTGGIGKSTLINTLVGLDFFSDTHFDI---------GTGKDSYEQIAGIVAY---- 645 Query: 93 VRQNIAFPLTLAKMRKADIAQKVSETAKILDLTNLLDRKPSQLSGGQRQRVAMGRAIVRH 152 ++ + R+AD + + D R R A GR + H Sbjct: 646 ---ELS---EMTAFRRADAEAVKAFFSSRKD----------------RYRGAYGRYVQDH 683 Query: 153 PK 154 P+ Sbjct: 684 PR 685

Rv1243c

Rv1255c

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
Rv1243c	0	16	1.371538	PE-PGRS family protein PE_PGRS23
Rv1244	-1	14	-0.580425	Probable lipoprotein LpqZ
Rv1245c	-2	11	-1.212105	Probable short-chain type
Rv1246c	-2	10	-0.337344	Toxin RelE
Rv1247c	-2	10	-0.171184	Antitoxin RelB
Rv1248c	-2	8	-0.335450	Multifunctional alpha-ketoglutarate metabolic
Rv1249c	-2	7	-0.070724	Possible membrane protein
Rv1250	-1	7	-0.087124	Probable drug-transport integral membrane
Rv1251c	-1	8	0.286764	Conserved hypothetical protein
Rv1252c	0	9	0.017417	Probable lipoprotein LprE
Rv1253	0	9	0.166700	Probable cold-shock DeaD-box protein A homolog
Rv1254	2	10	0.984676	Probable acyltransferase
Rv1255c	-1	9	0.522380	Probable transcriptional regulatory protein

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv1243c	FLAGELLIN	37	2e-04	Flagellin signature.
>FLAGELLIN#Flagellin signature. Length = 507 Score = 37.3 bits (86), Expect = 2e-04 Identities = 33/286 (11%), Positives = 51/286 (17%), Gaps = 15/286 (5%) Query: 260 NGTDAGSGSNAVNPGVGGGAG---------------GIGGDGTNLGQTDVSGGAGGDGGD 304 NG S N + VG G G+ G N + G + Sbjct: 132 NGVKVLSQDNQMKIQVGANDGETITIDLQKIDVKSLGLDGFNVNGPKEATVGDLKSSFKN 191 Query: 305 GANFASGGAGGNGGAAQSGFGDAVGGNGGAGGNGGAGGGGGLGGAGGSANVANAGNSIGG 364 + + G N G V G N + Sbjct: 192 VTGYDTYAVGANKYRVDVNSGAVVTDTTAPTVPDKVYVNAANGQLTTDDAENNTAVDLFK 251 Query: 365 NGGAGGNGGIGAPGGAGGAGGNANQDNPPGGNSTGGNGGAGGDGGVGASADVGGAGGFGG 424 + GG G + + G DG S + G Sbjct: 252 TTKSTAGTAEAKAIAGAIKGGKEGDTFDYKGVTFTIDTKTGNDGNGKVSTTINGEKVTLT 311 Query: 425 SGGRGGLLLGTGGAGGDGGVGGDGGIGAQGGSGGNGGNGGIGADGMANQDGDGGDGGNGG 484 A + + + + Sbjct: 312 VADITAGAANVDAATLQSSKNVYTSVVNGQFTFDDKTKNESAKLSDLEANNAVKGESKIT 371 Query: 485 DGGAGGAGGVGGNGGTGGAGGLFGQSGSPGSGAAGGLGGAGGNGGA 530 GA G+ T +F + G A Sbjct: 372 VNGAEYTANAAGDKVTLAGKTMFIDKTASGVSTLINEDAAAAKKST 417
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv1245c	DHBDHDRGNASE	74	8e-18	2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase signature. Length = 261 Score = 74.3 bits (182), Expect = 8e-18 Identities = 56/201 (27%), Positives = 92/201 (45%), Gaps = 2/201 (0%) Query: 2 EGFAGKVAVVTGAGSGIGQALAIELARSGAKVAISDVDTDGLADTEHRLKAISTPVKTDR 61 +G GK+A +TGA GIG+A+A LA GA +A D + + L LKA + + Sbjct: 4 KGIEGKIAFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHAEAFP 63 Query: 62 LDVTEREAFLAYADAVNEHFGTVNQIYNNAGIAFTGDIEVSQFKDIERVMDVDFWGVVNG 121 DV + A + G ++ + N AG+ G I ++ E V+ GV N Sbjct: 64 ADVRDSAAIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGVFNA 123 Query: 122 TKAFLPHLIASGDGHVINISSVFGLFSAPGQAAYNSAKFAVRGFTEALRQEMALAGHPVK 181 +++ +++ G ++ + S AAY S+K A FT+ L E LA + ++ Sbjct: 124 SRSVSKYMMDRRSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLE--LAEYNIR 181 Query: 182 VTTVHPGGVKTAIARNATAAE 202 V PG +T + + A E Sbjct: 182 CNIVSPGSTETDMQWSLWADE 202
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv1248c	PF03544	34	0.002	Gram-negative bacterial tonB protein
>PF03544#Gram-negative bacterial tonB protein Length = 243 Score = 34.2 bits (78), Expect = 0.002 Identities = 9/61 (14%), Positives = 17/61 (27%) Query: 42 PEPTSQPAAEPTRVTSPLVAERAAAAAPQAPPKPADTAAAGNGVVAALAAKTAVPPPAEG 101 PEP +P EP + ++ + P+ P + + A Sbjct: 76 PEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKPVKKVEQPKRDVKPVESRPASPFENTAPA 135 Query: 102 D 102 Sbjct: 136 R 136 Score = 32.3 bits (73), Expect = 0.011 Identities = 21/116 (18%), Positives = 31/116 (26%), Gaps = 6/116 (5%) Query: 38 VDYSPEPTSQPAAEPTRVTSPLVAERAAAAAPQAPPKPADTAAAGNGVVAALAAKTAVPP 97 V PEP +P EP + P E P K Sbjct: 66 VQPPPEPVVEPEPEPEPIPEP-PKEAPVVIEKPKPKPKPKPKPVKKVEQPKRDVKPVESR 124 Query: 98 PAEGDEVAVLRGAAAAVVKNMSASLEVPTATSVRAVPAKLLIDNRIVINNQLKRTR 153 PA E A A + +A+ + A + L N+ + + R Sbjct: 125 PASPFE-----NTAPARPTSSTATAATSKPVTSVASGPRALSRNQPQYPARAQALR 175
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv1250	TCRTETB	143	2e-39	Tetracycline resistance protein TetB signature.
>TCRTETB#Tetracycline resistance protein TetB signature. Length = 458 Score = 143 bits (363), Expect = 2e-39 Identities = 90/415 (21%), Positives = 173/415 (41%), Gaps = 15/415 (3%) Query: 12 SYFRNPWPALWAMMVGFFMIMLDSTVVAIANPTIMAQLRIGYATVVWVTSAYLLAYAVPM 71 S R+ +W ++ FF ++ + V+ ++ P I A+ WV +A++L +++ Sbjct: 8 SNLRHNQILIWLCILSFFSVL-NEMVLNVSLPDIANDFNKPPASTNWVNTAFMLTFSIGT 66 Query: 72 LVAGRLGDRFGPKNLYLIGLGVFTVASLGCGLS-SGAGMLIAARVVQGVGAGLLTPQTLS 130 V G+L D+ G K L L G+ + S+ + S +LI AR +QG GA + Sbjct: 67 AVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFFSLLIMARFIQGAGAAAFPALVMV 126 Query: 131 TITRIFPAHRRGVALGAWGTVASVASLVGPLAGGALVDSMGWEWIFFVNVPVGVIGLILA 190 + R P RG A G G++ ++ VGP GG + + W ++ + + + +I + Sbjct: 127 VVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGMIAHYIHWSYLLLIPM-ITIITVPFL 185 Query: 191 AYLIPALPHHPHRFDWFGVGLSGAGMFLIVFGLQQGQSANWQPWIWAVIVGGIGFMSLFV 250 L+ FD G+ L G+ + S + I +V+ +FV Sbjct: 186 MKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFT---TSYSISFLIVSVL-----SFLIFV 237 Query: 251 YWQARNAREPLIPLEVFNDRNFSLSNLRIAIIAFAGTGMMLPVTFYAQAVCGLSP-THTA 309 + P + + + F + L II G + V + + V LS + Sbjct: 238 KHIRKVTD-PFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVSMVPYMMKDVHQLSTAEIGS 296 Query: 310 VLFAPTAIVGGVLAPFVGMIIDRSHPLCVLGFGFSVLAIAMTWLLCEMAPGTPIWRLVLP 369 V+ P + + G+++DR PL VL G + L+++ +L T W + + Sbjct: 297 VIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSVS--FLTASFLLETTSWFMTII 354 Query: 370 FIALGVAGAFVWSPLTVTATRNLRPHLAGASSGVFNAVRQLGAVLGSASMAAFMT 424 + + +F + ++ + +L+ AGA + N L G A + ++ Sbjct: 355 IVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTSFLSEGTGIAIVGGLLS 409
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv1252c	cdtoxina	29	0.009	Cytolethal distending toxin A signature.
>cdtoxina#Cytolethal distending toxin A signature. Length = 258 Score = 29.3 bits (65), Expect = 0.009 Identities = 16/65 (24%), Positives = 24/65 (36%), Gaps = 3/65 (4%) Query: 17 VVAALVAATLTGCGSGDSTVAKTPEATPSLSTAHPAPPSSEPSP---PSATAAPPSNHSA 73 + L+ L GC SG + P+ P P PS + P A P+N + Sbjct: 10 IAGILIPILLNGCSSGKNKAYLDPKVFPPQVEGGPTVPSPDEPGLPLPGPGPALPTNGAI 69 Query: 74 APVDP 78 +P Sbjct: 70 PIPEP 74
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv1255c	HTHTETR	62	3e-14	TetR bacterial regulatory protein HTH signature.
>HTHTETR#TetR bacterial regulatory protein HTH signature. Length = 215 Score = 62.3 bits (151), Expect = 3e-14 Identities = 30/130 (23%), Positives = 49/130 (37%), Gaps = 3/130 (2%) Query: 8 SARRTELAADRILDAAERLFTQRDPASIGMNEIAKAAGCSRATLYRYFDSREALRTAYVH 67 + + + ILD A RLF+Q+ +S + EIAKAAG +R +Y +F + L + Sbjct: 5 TKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSEIWE 64 Query: 68 RETRRLG---REIMVKIADVVEPAERLLVSITTTLRMVRDNPALAAWFTTTRPPIGGEMA 124 +G E K R ++ + + L + GEMA Sbjct: 65 LSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVTEERRRLLMEIIFHKCEFVGEMA 124 Query: 125 GRSEVIAALA 134 + L Sbjct: 125 VVQQAQRNLC 134

Rv1473

Rv1488

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
Rv1473	-2	12	-0.744997	Probable macrolide-transport ATP-binding protein
Rv1473A	-2	11	-1.009950	Possible transcriptional regulatory protein
Rv1474c	-2	10	-0.957951	Probable transcriptional regulatory protein
Rv1475c	-2	9	-0.646685	Probable iron-regulated aconitate hydratase Acn
Rv1476	1	8	0.510991	Possible membrane protein
Rv1477	0	9	0.687340	Peptidoglycan hydrolase
Rv1478	1	8	0.270355	Possible invasion protein
Rv1479	2	9	0.023118	Probable transcriptional regulatory protein
Rv1480	0	8	0.102114	Conserved protein
Rv1481	0	8	-0.248001	Probable membrane protein
Rv1482c	-1	7	-0.096553	Conserved hypothetical protein
Rv1483	-1	11	0.094518	3-oxoacyl-[acyl-carrier protein] reductase FabG1
Rv1484	-1	14	0.124361	NADH-dependent enoyl-[acyl-carrier-protein]
Rv1485	-1	14	0.401063	Ferrochelatase HemZ (protoheme ferro-lyase)
Rv1486c	-2	17	-2.338212	Conserved hypothetical protein
Rv1487	-1	16	-2.323017	Conserved membrane protein
Rv1488	-3	11	-0.398826	Possible exported conserved protein

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv1473	BCTERIALGSPC	32	0.004	Bacterial general secretion pathway protein C signa...
>BCTERIALGSPC#Bacterial general secretion pathway protein C signature. Length = 272 Score = 32.2 bits (73), Expect = 0.004 Identities = 15/53 (28%), Positives = 25/53 (47%), Gaps = 2/53 (3%) Query: 82 ARDRVLSARGLDVLLTDLEKQQALMAEVADEDERDRAIRRYGQLEERFVALGG 134 D ++ GLD L D E+ + M +AD + R GQ ++ ++ GG Sbjct: 220 DNDMAVALNGLD--LRDAEQAKKAMERMADVHNFTLTVERDGQRQDIYMEFGG 270
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv1474c	HTHTETR	69	6e-17	TetR bacterial regulatory protein HTH signature.
>HTHTETR#TetR bacterial regulatory protein HTH signature. Length = 215 Score = 69.3 bits (169), Expect = 6e-17 Identities = 32/177 (18%), Positives = 63/177 (35%), Gaps = 14/177 (7%) Query: 1 MPKVSEDHLAARRRQILDGARRCFAEYGYDKATVRRLEQAIGMSRGAIFHHFRDKDALFF 60 M + ++ R+ ILD A R F++ G ++ + +A G++RGAI+ HF+DK LF Sbjct: 1 MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFS 60 Query: 61 ALAREDTERMAAVAS--------------REGLIGVMRDMLAAPDQFDWLATRLEIARKL 106 + + + RE LI V+ + + + + Sbjct: 61 EIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVTEERRRLLMEIIFHKCEFV 120 Query: 107 RNDPDFSRGWAERSAELAAATTDRLRRQKQANRVRDDVPSDVLRCYLDLVLDGLLAR 163 + E L+ +A + D+ + + + GL+ Sbjct: 121 GEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMTRRAAIIMRGYISGLMEN 177
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv1477	GPOSANCHOR	49	4e-08	Gram-positive coccus surface protein anchor signature.
>GPOSANCHOR#Gram-positive coccus surface protein anchor signature. Length = 539 Score = 48.5 bits (115), Expect = 4e-08 Identities = 33/199 (16%), Positives = 60/199 (30%), Gaps = 14/199 (7%) Query: 44 DTIAALIADVAKANQRLQDLSDEVQAEQESVNKAMVDVETARD-------NAAAAEDDLE 96 AAL A A+ + L+ + A+ + + A + Sbjct: 183 AEKAALEARQAELEKALEGAMNFSTADSAKIKTLEAEKAALAARKADLEKALEGAMNFST 242 Query: 97 VSQRAVKDANAAIAAAQHRFDTFAAATYMNGPSVSYLSASSPDEIIATVTAAKTLSASSQ 156 +K A AA + R A + S +I L A Sbjct: 243 ADSAKIKTLEAEKAALEARQAELEKALEGAMNFSTADS----AKIKTLEAEKAALEAEKA 298 Query: 157 AVMANLQRARTERVNTE---SAARLAKQKADKAAADAKASQDAAVAALTETRRKFDEQRE 213 + Q R + A+R AK++ + + + A+ RR D RE Sbjct: 299 DLEHQSQVLNANRQSLRRDLDASREAKKQLEAEHQKLEEQNKISEASRQSLRRDLDASRE 358 Query: 214 EVQRLAAERDAAQARLQAA 232 ++L AE + + + + Sbjct: 359 AKKQLEAEHQKLEEQNKIS 377 Score = 45.1 bits (106), Expect = 4e-07 Identities = 35/193 (18%), Positives = 67/193 (34%), Gaps = 8/193 (4%) Query: 46 IAALIADVAKANQRLQDLSDEVQAEQESVNKAMVDVETARDNAAAAEDDLEVSQRAVKDA 105 AAL A A + L+ + A+ + + A E LE + Sbjct: 220 KAALAARKADLEKALEGAMNFSTADSAKIKTLEAEKAALEARQAELEKALEGAMNFSTAD 279 Query: 106 NAAIAAAQHRFD----TFAAATYMNGPSVSYLSASSPDEIIATVTAAKTLSASSQAVMAN 161 +A I + A + + + + D + A+ A K L A Q + Sbjct: 280 SAKIKTLEAEKAALEAEKADLEHQSQVLNANRQSLRRD-LDASREAKKQLEAEHQKLEEQ 338 Query: 162 LQRARTERVNTE---SAARLAKQKADKAAADAKASQDAAVAALTETRRKFDEQREEVQRL 218 + + R + A+R AK++ + + + A+ RR D RE +++ Sbjct: 339 NKISEASRQSLRRDLDASREAKKQLEAEHQKLEEQNKISEASRQSLRRDLDASREAKKQV 398 Query: 219 AAERDAAQARLQA 231 + A ++L A Sbjct: 399 EKALEEANSKLAA 411 Score = 36.6 bits (84), Expect = 2e-04 Identities = 28/220 (12%), Positives = 62/220 (28%), Gaps = 27/220 (12%) Query: 36 LATADPQTDTIAALIADVAKANQRLQDLSDEVQAEQESVNKAMVDVETARDNAAAAEDDL 95 L+ + + A AD+ KA + + S A+ +++ + + + A + Sbjct: 108 LSEKASKIQELEARKADLEKALEGAMNFSTADSAKIKTLEAEKAALAARKADLEKALEGA 167 Query: 96 EVSQRAVKDANAAIAAAQHRFDTFAA---ATYMNGPSVSYLSASSPDEIIATVTAAKTLS 152 A + A + + A + S ++ + A A Sbjct: 168 MNFSTADSAKIKTLEAEKAALEARQAELEKALEGAMNFSTADSAKIKTLEAEKAALAARK 227 Query: 153 ASSQAVMANLQRARTERVNTESAARLAK-------------------QKADKAAADAKAS 193 A + +T +A++ + A + A Sbjct: 228 ADLEK-----ALEGAMNFSTADSAKIKTLEAEKAALEARQAELEKALEGAMNFSTADSAK 282 Query: 194 QDAAVAALTETRRKFDEQREEVQRLAAERDAAQARLQAAR 233 A + + + Q L A R + + L A+R Sbjct: 283 IKTLEAEKAALEAEKADLEHQSQVLNANRQSLRRDLDASR 322
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv1479	HTHFIS	33	0.002	FIS bacterial regulatory protein HTH signature.
>HTHFIS#FIS bacterial regulatory protein HTH signature. Length = 484 Score = 32.9 bits (75), Expect = 0.002 Identities = 32/150 (21%), Positives = 56/150 (37%), Gaps = 28/150 (18%) Query: 54 IVGQD----QLVERMLVGLLSKGHVLLEGVPGVAKTL---AVETFARVVGGTFSRIQ--- 103 +VG+ ++ + + + +++ G G K L A+ + + G F I Sbjct: 139 LVGRSAAMQEIYRVLARLMQTDLTLMITGESGTGKELVARALHDYGKRRNGPFVAINMAA 198 Query: 104 FTPDLVPTDIIGTRI--------YRQGREEFDTELGPVVAN--FLLADEINRAPAKVQSA 153 DL+ +++ G GR E A L DEI P Q+ Sbjct: 199 IPRDLIESELFGHEKGAFTGAQTRSTGRFE--------QAEGGTLFLDEIGDMPMDAQTR 250 Query: 154 LLEVMQERHVSIGGRTFPMPSPFLVMATQN 183 LL V+Q+ + G P+ S ++A N Sbjct: 251 LLRVLQQGEYTTVGGRTPIRSDVRIVAATN 280
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv1481	TCRTETB	29	0.022	Tetracycline resistance protein TetB signature.
>TCRTETB#Tetracycline resistance protein TetB signature. Length = 458 Score = 29.5 bits (66), Expect = 0.022 Identities = 19/112 (16%), Positives = 39/112 (34%), Gaps = 16/112 (14%) Query: 4 PLLGPMTLSGFAHSWFFLFLF----VVAGLVALYILMQLARQRRMLRFANMELLES---- 55 P +G M W +L L ++ + +L + R + + L+ Sbjct: 156 PAIGGMIAHYI--HWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVF 213 Query: 56 -VAPKRPSRWRHVPAILLVLSLLLFTIAMAGPTH---DVRIPRNRAVVMLVI 103 + + I+ VLS L+F + T D + +N ++ V+ Sbjct: 214 FMLFTTSYSISFL--IVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVL 263
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv1483	DHBDHDRGNASE	116	1e-33	2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase signature. Length = 261 Score = 116 bits (291), Expect = 1e-33 Identities = 70/252 (27%), Positives = 124/252 (49%), Gaps = 21/252 (8%) Query: 16 RSVLVTGGNRGIGLAIAQRLAADGHKVAVTHRGSGAPKGLFGVE-----------CDVTD 64 + +TG +GIG A+A+ LA+ G +A + + DV D Sbjct: 9 KIAFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHAEAFPADVRD 68 Query: 65 SDAVDRAFTAVEEHQGPVEVLVSNAGLSADAFLMRMTEEKFEKVINANLTGAFRVAQRAS 124 S A+D +E GP+++LV+ AG+ + +++E++E + N TG F ++ S Sbjct: 69 SAAIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGVFNASRSVS 128 Query: 125 RSMQRNKFGRMIFIGSVSGSWGIGNQANYAASKAGVIGMARSIARELSKANVTANVVAPG 184 + M + G ++ +GS + A YA+SKA + + + EL++ N+ N+V+PG Sbjct: 129 KYMMDRRSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLELAEYNIRCNIVSPG 188 Query: 185 YIDTDMTRAL-------DERIQQGALQF---IPAKRVGTPAEVAGVVSFLASEDASYISG 234 +TDM +L ++ I+ F IP K++ P+++A V FL S A +I+ Sbjct: 189 STETDMQWSLWADENGAEQVIKGSLETFKTGIPLKKLAKPSDIADAVLFLVSGQAGHITM 248 Query: 235 AVIPVDGGMGMG 246 + VDGG +G Sbjct: 249 HNLCVDGGATLG 260
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv1484	DHBDHDRGNASE	44	2e-07	2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase signature. Length = 261 Score = 44.3 bits (104), Expect = 2e-07 Identities = 54/272 (19%), Positives = 100/272 (36%), Gaps = 32/272 (11%) Query: 5 LDGKRILVSGIITDSSIAFHIARVAQEQGAQLVLTGFD----RLRLIQRITDRLPAKAPL 60 ++GK ++G I +AR QGA + D +L + A Sbjct: 6 IEGKIAFITG--AAQGIGEAVARTLASQGAHI--AAVDYNPEKLEKVVSSLKAEARHAEA 61 Query: 61 LELDVQNEEHLASLAGRVTEAIGAGNKLDGVVHSIGFMPQTGMGINPFFDAPYADVSKGI 120 DV++ + + R+ +G +D +V+ G + + Sbjct: 62 FPADVRDSAAIDEITARIEREMG---PIDILVNVAGVLR-----PGLIHSLSDEEWEATF 113 Query: 121 HISAYSYASMAKALLPIM--NPGGSIVGMDFD----PSRAMPAYNWMTVAKSALESVNRF 174 +++ + ++++ M GSIV + + P +M AY +K+A + Sbjct: 114 SVNSTGVFNASRSVSKYMMDRRSGSIVTVGSNPAGVPRTSMAAYA---SSKAAAVMFTKC 170 Query: 175 VAREAGKYGVRSNLVAAGPIRTLAMSAIVGGALGEEAGAQIQLLEEGWDQRAPIGWNMKD 234 + E +Y +R N+V+ G T ++ G E I+ E + P+ K Sbjct: 171 LGLELAEYNIRCNIVSPGSTETDMQWSLWADENGAE--QVIKGSLETFKTGIPLK---KL 225 Query: 235 ATP--VAKTVCALLSDWLPATTGDIIYADGGA 264 A P +A V L+S T + DGGA Sbjct: 226 AKPSDIADAVLFLVSGQAGHITMHNLCVDGGA 257
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv1488	IGASERPTASE	29	0.047	IgA-specific serine endopeptidase (S6) signature.
>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature. Length = 1541 Score = 28.9 bits (64), Expect = 0.047 Identities = 34/211 (16%), Positives = 65/211 (30%), Gaps = 24/211 (11%) Query: 158 LRVARVELRSIDPPPS---IQASMEKQMKADREKRAMILTAEGTREAAIKQAEGQKQAQI 214 VA+ + + + A++EK+ KA E Q + +Q+ Sbjct: 1083 NEVAQSGSETKETQTTETKETATVEKEEKAKVETEK-------------TQEVPKVTSQV 1129 Query: 215 LAAEGAKQAAILAAEADRQSRMLRAQGERAAAYLQAQGQAKAIEKTFAAIKAGRPTPEML 274 + + AE R++ E Q+Q A + A + + Sbjct: 1130 SPKQEQSETVQPQAEPARENDPTVNIKEP-----QSQTNTTADTEQPAKETSSNVEQPVT 1184 Query: 275 AYQYLQTLPEMARGDANKVWVVPSDFNAALQGFTRLLGKPGEDGVFRFEPSPVEDQPKHA 334 + T + N P+ + + K R P VE + Sbjct: 1185 ESTTVNTGNSVVE---NPENTTPATTQPTVNSESSNKPKNRHRRSVRSVPHNVEPATTSS 1241 Query: 335 ADGDDAEVAGWFSTDTDPSIARAVATAEAIA 365 D + ST+T+ ++ A A A+ +A Sbjct: 1242 NDRSTVALCDLTSTNTNAVLSDARAKAQFVA 1272

Rv1680

Rv1686c

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
Rv1680	-1	11	-0.840196	Hypothetical protein
Rv1681	-1	11	-0.984863	Possible molybdopterin biosynthesis protein
Rv1682	0	11	-1.643605	Probable coiled-coil structural protein
Rv1683	1	10	-0.330308	Possible bifunctional enzyme; long-chain
Rv1684	-1	12	-0.735854	Conserved hypothetical protein
Rv1685c	-1	14	-1.141159	Conserved hypothetical protein
Rv1686c	-1	13	0.076475	Probable conserved integral membrane protein ABC

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv1680	VACJLIPOPROT	30	0.006	VacJ lipoprotein signature.
>VACJLIPOPROT#VacJ lipoprotein signature. Length = 251 Score = 30.3 bits (68), Expect = 0.006 Identities = 17/84 (20%), Positives = 29/84 (34%), Gaps = 12/84 (14%) Query: 4 EPLVVGAVAYTPNVVPIWEGIRGYFQDSESPDTQMDFVLYSNYARLVDSL---------- 53 P+ V Y P P G+ + + E P +++ L + + + Sbjct: 53 RPVAVAWRDYVPQ--PARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTRFFLNTILG 110 Query: 54 IAGHIDIAWNTNLAYVRTVLQTGG 77 + G ID+A N RT G Sbjct: 111 MGGFIDVAGMANPKLQRTEPHRFG 134
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv1681	PF01206	29	0.006	SirA family protein
>PF01206#SirA family protein Length = 76 Score = 29.0 bits (65), Expect = 0.006 Identities = 9/59 (15%), Positives = 21/59 (35%), Gaps = 1/59 (1%) Query: 2 IIELMRRVVGLAQGATAEVAVYGDRDRDLAERWCANTGNTLVRADVDQTGVGTLVVRRG 60 I++ + + + G V E + TG+ L+ ++ G ++R Sbjct: 19 ILKAKKTLATMNAGEVLYVMATDPGSVKDFESFSKQTGHELLEQK-EEDGTYHFRLKRA 76
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv1685c	HTHTETR	71	3e-17	TetR bacterial regulatory protein HTH signature.
>HTHTETR#TetR bacterial regulatory protein HTH signature. Length = 215 Score = 70.8 bits (173), Expect = 3e-17 Identities = 29/121 (23%), Positives = 46/121 (38%), Gaps = 11/121 (9%) Query: 13 RPAGSSDTRERILSSARELFAHNGIDRTSIRAVAAKAGVDAALVHHYFGTKQQLFAAAIH 72 + +TR+ IL A LF+ G+ TS+ +A AGV ++ +F K LF+ Sbjct: 5 TKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSEIWE 64 Query: 73 IPIDPMVIIGPIREAPVEELGYKLPSLLLPIWDSELGAGLIATLRSLISGSDVGLARSFL 132 + I E +E K P L + LI L S ++ L + Sbjct: 65 LSES------NIGELELEYQA-KFPGDPLSVL----REILIHVLESTVTEERRRLLMEII 113 Query: 133 E 133 Sbjct: 114 F 114
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv1686c	ABC2TRNSPORT	48	6e-09	ABC-2 type transport system membrane protein signat...
>ABC2TRNSPORT#ABC-2 type transport system membrane protein signature. Length = 262 Score = 48.4 bits (115), Expect = 6e-09 Identities = 38/172 (22%), Positives = 74/172 (43%), Gaps = 2/172 (1%) Query: 53 TMQRERASGTLERILTTPLRRLDLLAGYGTAFSIAAAAQATLACIVAFWFLGFDTAGSPV 112 R T E +L T LR D++ G A++ AA A V LG+ S + Sbjct: 90 AFGRMEGQRTWEAMLYTQLRLGDIVLGE-MAWAATKAALAGAGIGVVAAALGYTQWLSLL 148 Query: 113 WVFAIAIVNAVLGVGLGLLCSAFARTEFQAVQFIPLVMVPQLLLAGIIVPRALMPTWLEW 172 + + + + LG++ +A A + + + LV+ P L L+G + P +P + Sbjct: 149 YALPVIALTGLAFASLGMVVTALAPSYDYFIFYQTLVITPILFLSGAVFPVDQLPIVFQT 208 Query: 173 ISNVMPASYALEALQQVGAHPELTGIAVRDVVVVLSFAVASLCLAAVTLRRR 224 + +P S++++ ++ + + + V + + V L+ LRRR Sbjct: 209 AARFLPLSHSIDLIRPIMLGHPVVDVCQH-VGALCIYIVIPFFLSTALLRRR 259

Rv1816

Rv1822

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
Rv1816	-2	9	0.738457	Possible transcriptional regulatory protein
Rv1817	-1	9	0.529489	Possible flavoprotein
Rv1818c	-2	9	0.913296	PE-PGRS family protein PE_PGRS33
Rv1819c	-1	9	-1.404665	Probable drug-transport transmembrane
Rv1820	-1	10	-1.035426	Probable acetolactate synthase IlvG
Rv1821	1	12	-2.142926	Possible preprotein translocase ATPase SecA2
Rv1822	1	18	-2.669416	Probable

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv1816	HTHTETR	53	1e-10	TetR bacterial regulatory protein HTH signature.
>HTHTETR#TetR bacterial regulatory protein HTH signature. Length = 215 Score = 53.1 bits (127), Expect = 1e-10 Identities = 22/160 (13%), Positives = 50/160 (31%), Gaps = 6/160 (3%) Query: 8 GKRRDAREQIEAKIVELGRRQLLDHGAAGLSLRAIARNLGMVSSAVYRYVSSRDELLTLL 67 K + ++ I+++ R G + SL IA+ G+ A+Y + + +L + + Sbjct: 3 RKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSEI 62 Query: 68 LVDAYSDLADTVDRARDDTVADSWSDDVIAIARAVRGWAVTNPARWALL----YGSPVPG 123 + S++ + + D S + + VT R L+ + G Sbjct: 63 WELSESNIGELELEYQAKFPGDPLSVLREILIHVLE-STVTEERRRLLMEIIFHKCEFVG 121 Query: 124 YHAPPDRT-AGVATRVVGAFFDAIAAGIATGDIRLTDDVA 162 A + + + I + Sbjct: 122 EMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMTR 161
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv1818c	cloacin	37	1e-04	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 37.4 bits (86), Expect = 1e-04 Identities = 38/118 (32%), Positives = 46/118 (38%), Gaps = 11/118 (9%) Query: 158 GAAGLFGNGGAGGAGGNVASGTAGFGGAGGA--GGLLYGAGGAGGAGGRAGGGVGGIGGA 215 G G N GA GN+ G G G GGA G G G +G GG G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 216 GGAGGNGGLLFGAGGAGGVGGLAADAGDGGAGGDGGLFFGVGGAGGAGGTGTNVTGGA 273 G GGNG G+G G + +AA G F GAGG +++ GA Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFG---------FPALSTPGAGGLAVSISAGA 111 Score = 37.4 bits (86), Expect = 1e-04 Identities = 27/82 (32%), Positives = 30/82 (36%) Query: 262 AGGTGTNVTGGAGGAGGNGGLLFGAGGVGGVGGDGVAFLGTAPGGPGGAGGAGGLFGVGG 321 +GG G GA GN GVGG DG + GG+G G G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 322 AGGAGGIGLVGNGGAGGSGGSA 343 G GG G G G G SA Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSA 83 Score = 33.5 bits (76), Expect = 0.002 Identities = 31/101 (30%), Positives = 38/101 (37%), Gaps = 2/101 (1%) Query: 140 GNGGAGGSGAAGMPGGNGGAAGLFGNGGAGGAGGNVASGTAGFGGAGGAGGLLYGAGGAG 199 G+G +GA G G G GG G +S +GG G+G G G G Sbjct: 4 GDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHG 63 Query: 200 GAGGRAGGGVGGIGGAGGAGGNGGLLFG--AGGAGGVGGLA 238 GG G G G + + FG A G GGLA Sbjct: 64 NGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 32.4 bits (73), Expect = 0.004 Identities = 25/81 (30%), Positives = 33/81 (40%) Query: 408 GAGGAGGFGFGGAGGAGGLGGKAGLIGDGGDGGAGGNGTGAKGGDGGAGGGAILVGNGGN 467 GA G GG G G GG + G + G G+G+ GG G GNG + Sbjct: 12 GAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNGNS 71 Query: 468 GGNAGSGTPNGSAGTGGAGGL 488 GG +G+G + A G Sbjct: 72 GGGSGTGGNLSAVAAPVAFGF 92 Score = 32.4 bits (73), Expect = 0.005 Identities = 32/107 (29%), Positives = 35/107 (32%), Gaps = 5/107 (4%) Query: 321 GAGGAGGIGLVGNGGAGGSGGSALLWGDGGAGGAGGVGSTTGGAGGAGGNAGLLVGAGGA 380 G G G + +GG L GGA G S GG G+ G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 381 GGAGALGGGATGVGGAGGNGGTAGLLFGAGGAGGFGFGGAGGAGGLG 427 G G G G G G A A A GF GAGGL Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVA-----APVAFGFPALSTPGAGGLA 104 Score = 32.0 bits (72), Expect = 0.005 Identities = 26/79 (32%), Positives = 31/79 (39%) Query: 380 AGGAGALGGGATGVGGAGGNGGTAGLLFGAGGAGGFGFGGAGGAGGLGGKAGLIGDGGDG 439 +GG G NGG GL G G + G G+ G G +G+ GG G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 440 GAGGNGTGAKGGDGGAGGG 458 G G G GG G GG Sbjct: 62 HGNGGGNGNSGGGSGTGGN 80 Score = 31.6 bits (71), Expect = 0.008 Identities = 22/72 (30%), Positives = 30/72 (41%) Query: 120 NGANGAPGTGANGGDGGILIGNGGAGGSGAAGMPGGNGGAAGLFGNGGAGGAGGNVASGT 179 N + NGG G+ +G G + GSG + GG +G + G G GN Sbjct: 10 NTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNG 69 Query: 180 AGFGGAGGAGGL 191 GG+G G L Sbjct: 70 NSGGGSGTGGNL 81 Score = 30.8 bits (69), Expect = 0.014 Identities = 40/133 (30%), Positives = 43/133 (32%), Gaps = 2/133 (1%) Query: 293 GGDGVAFLGTAPGGPGGAGGAGGLFGVGGAGGAGGIGLVGNGGAGGSGGSALLWG--DGG 350 GGDG A G G GVGG G N GG GS + WG G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 351 AGGAGGVGSTTGGAGGAGGNAGLLVGAGGAGGAGALGGGATGVGGAGGNGGTAGLLFGAG 410 G G S G G +A A G G G V + G A A Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAAIADIMAA 122 Query: 411 GAGGFGFGGAGGA 423 G F FG G A Sbjct: 123 LKGPFKFGLWGVA 135 Score = 30.1 bits (67), Expect = 0.026 Identities = 21/71 (29%), Positives = 26/71 (36%) Query: 362 GGAGGAGGNAGLLVGAGGAGGAGALGGGATGVGGAGGNGGTAGLLFGAGGAGGFGFGGAG 421 GA GN G GG + G G + G G +G+ +G G G G G Sbjct: 11 TGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNGN 70 Query: 422 GAGGLGGKAGL 432 GG G L Sbjct: 71 SGGGSGTGGNL 81
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv1821	SECA	882	0.0	SecA protein signature.
>SECA#SecA protein signature. Length = 901 Score = 882 bits (2280), Expect = 0.0 Identities = 285/815 (34%), Positives = 401/815 (49%), Gaps = 116/815 (14%) Query: 48 RLLGASTEKNRSRSLADVTASAEYDKEAADLSDEKLR-KAAGLLNLDDLAESAD--IPQF 104 ++ G+ ++ R V + E LSDE+L+ K A + E + IP+ Sbjct: 8 KVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELKGKTAEFRARLEKGEVLENLIPEA 67 Query: 105 LAIAREAAERRTGLRPFDVQLLGALRMLAGDVIEMATGEGKTLAGAIAAAGYALAGRHVH 164 A+ REA++R G+R FDVQLLG + + + EM TGEGKTL + A AL G+ VH Sbjct: 68 FAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEMRTGEGKTLTATLPAYLNALTGKGVH 127 Query: 165 VVTINDYLARRDAEWMGPLLDAMGLTVGWITADSTPDERRTAYDRDVTYASVNEIGFDVL 224 VVT+NDYLA+RDAE PL + +GLTVG +R AY D+TY + NE GFD L Sbjct: 128 VVTVNDYLAQRDAENNRPLFEFLGLTVGINLPGMPAPAKREAYAADITYGTNNEYGFDYL 187 Query: 225 RDQLVTDVNDLVSPNPDVALIDEADSVLVDEALVPLVLAGTTHRETPRLEII-RLVAELV 283 RD + + V AL+DE DS+L+DEA PL+++G + + + +++ L+ Sbjct: 188 RDNMAFSPEERVQRKLHYALVDEVDSILIDEARTPLIISGPAEDSSEMYKRVNKIIPHLI 247 Query: 284 GDKD-------ADEYFATDSDNRNVHLTEHGARKVEKAL-------GGIDLYSEEHVGTT 329 + + +F+ D +R V+LTE G +E+ L G LYS ++ Sbjct: 248 RQEKEDSETFQGEGHFSVDEKSRQVNLTERGLVLIEELLVKEGIMDEGESLYSPANI-ML 306 Query: 330 LTEVNVALHAHVLLQRDVHYIVRDDAVHLINASRGRIAQLQRWPDGLQAAVEAKEGIETT 389 + V AL AH L RDV YIV+D V +++ GR Q +RW DGL AVEAKEG++ Sbjct: 307 MHHVTAALRAHALFTRDVDYIVKDGEVIIVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQ 366 Query: 390 ETGEVLDTITVQALINRYATVCGMTGTALAAGEQLRQFYQLGVSPIPPNKPNIREDEADR 449 + L +IT Q Y + GMTGTA + Y+L +P N+P IR+D D Sbjct: 367 NENQTLASITFQNYFRLYEKLAGMTGTADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDL 426 Query: 450 VYITTAAKNDGIVEHITEVHQRGQPVLVGTRDVAESEELHERLVRRGVPAVVLNAKNDAE 509 VY+T A K I+E I E +GQPVLVGT + +SE + L + G+ VLNAK A Sbjct: 427 VYMTEAEKIQAIIEDIKERTAKGQPVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHAN 486 Query: 510 EARVIAEAGKYGAVTVSTQMAGRGTDIRLGGSDEA----------------------DHD 547 EA ++A+AG AVT++T MAGRGTDI LGGS +A HD Sbjct: 487 EAAIVAQAGYPAAVTIATNMAGRGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHD 546 Query: 548 RVAELGGLHVVGTGRHHTERLDNQLRGRAGRQGDPGSSVFFSSWEDDV--------VAAN 599 V E GGLH++GT RH + R+DNQLRGR+GRQGD GSS F+ S ED + V+ Sbjct: 547 AVLEAGGLHIIGTERHESRRIDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGM 606 Query: 600 LDHNKLPMATDENGRIVSPRTGSLLDHAQRVAEGRLLDVHANTWRYNQLIAQQRAIIVER 659 + KL M E I P + +AQR E R D+ Y+ + QR I + Sbjct: 607 M--RKLGMKPGE--AIEHPWVTKAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQ 662 Query: 660 RNTLLRTVTAREEL-------------AELAPKRYEELSD-------------------- 686 RN LL E + A + P+ EE+ D Sbjct: 663 RNELLDVSDVSETINSIREDVFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAE 722 Query: 687 ------KVSEERL-ETIC-----------------------RQIMLYHLDRGWADHLAYL 716 ++ EE L E I + +ML LD W +HLA + Sbjct: 723 WLDKEPELHEETLRERILAQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAM 782 Query: 717 ADIRESIHLRALGRQNPLDEFHRMAVDAFASLAAD 751 +R+ IHLR +++P E+ R + FA++ Sbjct: 783 DYLRQGIHLRGYAQKDPKQEYKRESFSMFAAMLES 817
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv1822	TYPE3IMSPROT	30	0.007	Type III secretion system inner membrane S protein ...
>TYPE3IMSPROT#Type III secretion system inner membrane S protein family signature. Length = 354 Score = 29.7 bits (67), Expect = 0.007 Identities = 12/88 (13%), Positives = 32/88 (36%), Gaps = 14/88 (15%) Query: 135 FGFMVGFPTILLGQCDPLWS----HVLLACGWAFLIWGMYAYLWAFVLYAVQMTM----V 186 ++ PT + PL +++ C F++ + Y + + Y ++ M + Sbjct: 162 LVTLLQLPTCGIECITPLLGQILRQLMVICTVGFVVISIADYAFEYYQYIKELKMSKDEI 221 Query: 187 VRQM------PKLKGRAHRPAAQNAGER 208 R+ P++K + + + Sbjct: 222 KREYKEMEGSPEIKSKRRQFHQEIQSRN 249

Rv1876

Rv1883c

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
Rv1876	-1	12	-2.433902	Probable bacterioferritin BfrA
Rv1877	-1	12	-1.881992	Probable conserved integral membrane protein
Rv1878	-3	15	-2.143977	Probable glutamine synthetase GlnA3 (glutamine
Rv1879	-1	15	-2.483028	Conserved hypothetical protein
Rv1880c	-2	16	-2.278234	Probable cytochrome P450 140 Cyp140
Rv1881c	0	18	-2.339150	Possible conserved lipoprotein LppE
Rv1882c	0	15	-1.429246	Probable short-chain type
Rv1883c	1	14	-1.920536	Conserved hypothetical protein

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv1876	HELNAPAPROT	27	0.026	Helicobacter neutrophil-activating protein A family ...
>HELNAPAPROT#Helicobacter neutrophil-activating protein A family signature. Length = 153 Score = 26.8 bits (59), Expect = 0.026 Identities = 20/105 (19%), Positives = 40/105 (38%), Gaps = 13/105 (12%) Query: 47 ESFDEMR-----HAEEITDRILLLDGLPN--YQRIGSLR------IGQTLREQFEADLAI 93 E F+E+ + I +R+L + G P + + E +A + Sbjct: 48 EKFEELYDHAAETVDTIAERLLAIGGQPVATVKEYTEHASITDGGNETSASEMVQALVND 107 Query: 94 EYDVLNRLKPGIVMCREKQDTTSAVLLEKIVADEEEHIDYLETQL 138 + + K I + E QD +A L ++ + E+ + L + L Sbjct: 108 YKQISSESKFVIGLAEENQDNATADLFVGLIEEVEKQVWMLSSYL 152
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv1877	TCRTETB	140	7e-38	Tetracycline resistance protein TetB signature.
>TCRTETB#Tetracycline resistance protein TetB signature. Length = 458 Score = 140 bits (354), Expect = 7e-38 Identities = 96/424 (22%), Positives = 186/424 (43%), Gaps = 18/424 (4%) Query: 22 SPVRRNIIFTALVFGVLVAATGQTIVVPALPTIVAELGSTVDQ-SWAVTSYLLGGTVVVV 80 S +R N I L + + ++ +LP I + +W T+++L ++ Sbjct: 8 SNLRHNQILIWLCILSFFSVLNEMVLNVSLPDIANDFNKPPASTNWVNTAFMLTFSIGTA 67 Query: 81 VAGKLGDLLGRNRVLLGSVVVFVVGSVLCGLSQTMTMLAI-SRALQGVGAGAISVTAYAL 139 V GKL D LG R+LL +++ GSV+ + + L I +R +QG GA A + Sbjct: 68 VYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFFSLLIMARFIQGAGAAAFPALVMVV 127 Query: 140 AAEVVPLRDRGRYQGVLGAVFGVNTVTGPLLGGWLTDYLSWRWAFWINVPVSIAVLTVAA 199 A +P +RG+ G++G++ + GP +GG + Y+ W++ + +P+ + Sbjct: 128 VARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGMIAHYIH--WSYLLLIPMITIITVPFL 185 Query: 200 TAVPALARPPKPVIDYLGILVIAVATTALIMATSWGGTTYAWGSATIVGLLIGAAVALGF 259 + K D GI++++V ++ T T+Y+ LI + ++ Sbjct: 186 MKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFT----TSYSI------SFLIVSVLSFLI 235 Query: 260 FVWLEGRAAAAILPPRLFGSPVFAVCCVLSFVVGFAMLGALTFVPIYLGYVDGAS-ATAS 318 FV + + P L + F + + ++ + G ++ VP + V S A Sbjct: 236 FVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVSMVPYMMKDVHQLSTAEIG 295 Query: 319 GLRTLPMVIGLLIASTGTGVLVGRTGRYKIFPVAGMALMAVAFLLMSQMDEWTPPLLQSL 378 + P + ++I G+LV R G + + G+ ++V+FL S + E T ++ Sbjct: 296 SVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNI-GVTFLSVSFLTASFLLETT-SWFMTI 353 Query: 379 YLVVLGAGIGLSMQVLVLIVQNTSSFEDLGVATSGVTFFRVVGASFGTATFGALF-VNFL 437 +V + G+ + V+ IV ++ ++ G S + F + G A G L + L Sbjct: 354 IIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTSFLSEGTGIAIVGGLLSIPLL 413 Query: 438 DRRL 441 D+RL Sbjct: 414 DQRL 417
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv1882c	DHBDHDRGNASE	64	3e-14	2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase signature. Length = 261 Score = 64.3 bits (156), Expect = 3e-14 Identities = 58/223 (26%), Positives = 93/223 (41%), Gaps = 10/223 (4%) Query: 2 KAIFITGAGSGMGREGATLFHANGWRVGAIDRNEDGLAALRVQLGAERLWARA--VDVTD 59 K FITGA G+G A + G + A+D N + L + L AE A A DV D Sbjct: 9 KIAFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHAEAFPADVRD 68 Query: 60 KAALEGALADFCAGNVGGGLDMMWNNAGIGEGGWFEDVPYEAAVRVVDVNFKAVLTGAYA 119 AA++ A G +D++ N AG+ G + E VN V + + Sbjct: 69 SAAIDEITARIEREM--GPIDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGVFNASRS 126 Query: 120 ALPYLKKAPGSLMFSTSSSSGTYGMPR--IAVYSATKHAVKGLTEALSVEWQRHGVRVAD 177 Y+ + + S+ G+PR +A Y+++K A T+ L +E + +R Sbjct: 127 VSKYMMDRRSGSIVTVGSNPA--GVPRTSMAAYASSKAAAVMFTKCLGLELAEYNIRCNI 184 Query: 178 VLPGLIDTAILTS--TRQHSDEGPYTISAEQIRAAAPKKGMFR 218 V PG +T + S ++ E S E + P K + + Sbjct: 185 VSPGSTETDMQWSLWADENGAEQVIKGSLETFKTGIPLKKLAK 227
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv1883c	TCRTETOQM	27	0.040	Tetracycline resistance protein TetO/TetQ/TetM family ...
>TCRTETOQM#Tetracycline resistance protein TetO/TetQ/TetM family signature. Length = 639 Score = 26.7 bits (59), Expect = 0.040 Identities = 8/30 (26%), Positives = 15/30 (50%) Query: 120 TVYYRVFGGWLRQRRNIRDMTKTLQRIKDL 149 Y R++ G L R ++R K +I ++ Sbjct: 265 LAYIRLYSGVLHLRDSVRISEKEKIKITEM 294

Rv2041c

Rv2048c

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
Rv2041c	-2	10	-0.405815	Probable sugar-binding lipoprotein
Rv2042c	-2	8	0.738443	Conserved protein
Rv2043c	1	17	1.344331	Pyrazinamidase/nicotinamidase PncA (PZase)
Rv2044c	1	17	1.467839	Conserved hypothetical protein
Rv2045c	1	17	1.484009	Carboxylesterase LipT
Rv2046	0	17	1.585892	Probable lipoprotein LppI
Rv2047c	0	16	1.794154	Conserved hypothetical protein
Rv2048c	0	17	1.827188	Polyketide synthase Pks12

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv2041c	MALTOSEBP	48	4e-08	Maltose binding protein signature.
>MALTOSEBP#Maltose binding protein signature. Length = 396 Score = 48.2 bits (114), Expect = 4e-08 Identities = 62/267 (23%), Positives = 110/267 (41%), Gaps = 33/267 (12%) Query: 79 QQLATFCAGGKCPDVLMAWELTYAELADRGVLLDLNTLLARDQAFAAELKSDSIGALYET 138 ++ A G PD++ + A G+L ++ D+AF +L + ++ Sbjct: 71 EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITP----DKAFQDKLYPFT----WDA 122 Query: 139 FTFNGGQYAFPEQWSGNFLFYNKQLFDDAGVPPPPGSWERPWSFAEFLDAAQALTKQGRS 198 +NG A+P L YNK L +P PP +WE E + L +G+S Sbjct: 123 VRYNGKLIAYPIAVEALSLIYNKDL-----LPNPPKTWE------EIPALDKELKAKGKS 171 Query: 199 GRDRQWGFVNAWVSFYAAGLFAMNNGVPWSVP--RMNPTHLNFDHDGFLEAVQFYADL-T 255 N ++ L A + G + + + + D+ G + F DL Sbjct: 172 AL-----MFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIK 226 Query: 256 NKHKVAPSAAEQQSMSTADLFSVGKAGIALAGHWRYQTFDRADGLDFDVAPLPIGPRGRA 315 NKH +A S++ A F+ G+ + + G W + D + +++ V LP +G+ Sbjct: 227 NKHM---NADTDYSIAEA-AFNKGETAMTINGPWAWSNIDTSK-VNYGVTVLPTF-KGQP 280 Query: 316 ACSDIGVTGLAIAATSRRKDQAWEFVK 342 + +GV I A S K+ A EF++ Sbjct: 281 SKPFVGVLSAGINAASPNKELAKEFLE 307
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv2043c	ISCHRISMTASE	31	0.003	Isochorismatase signature.
>ISCHRISMTASE#Isochorismatase signature. Length = 312 Score = 30.8 bits (69), Expect = 0.003 Identities = 22/80 (27%), Positives = 32/80 (40%), Gaps = 7/80 (8%) Query: 103 YSGFEGVDENGTPLLNWLRQRGVDEVDVVGIATDHCVRQTAEDAVRNGLATRVLVDLTAG 162 YS F+ T LL +R+ G D++ + GI TA +A + + D A Sbjct: 126 YSAFKR-----TNLLEMMRKEGRDQLIITGIYAHIGCLVTACEAFMEDIKAFFVGDAVAD 180 Query: 163 VSADTTVAALEEM--RTASV 180 S + ALE R A Sbjct: 181 FSLEKHQMALEYAAGRCAFT 200
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv2047c	PHPHTRNFRASE	65	5e-13	Phosphoenolpyruvate-protein phosphotransferase sign...
>PHPHTRNFRASE#Phosphoenolpyruvate-protein phosphotransferase signature. Length = 572 Score = 65.2 bits (159), Expect = 5e-13 Identities = 26/96 (27%), Positives = 44/96 (45%), Gaps = 2/96 (2%) Query: 743 GGRVRGRVRIVRPETIDDLQPGEILVAEVTDVGYTAAF--CYAAAVVTELGGPMSHAAVV 800 RV G + V ++ + +++AE TA + T++GG SH+A++ Sbjct: 135 SKRVLGHLIGVETGSLATIAEETVIIAEDLTPSDTAQLNKQFVKGFATDIGGRTSHSAIM 194 Query: 801 AREFGFPCVVDAQGATRFLPPGALVEVDGATGEIHV 836 +R P VV + T + G +V VDG G + V Sbjct: 195 SRSLEIPAVVGTKEVTEKIQHGDMVIVDGIEGIVIV 230
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv2048c	NUCEPIMERASE	40	2e-04	Nucleotide sugar epimerase signature.
>NUCEPIMERASE#Nucleotide sugar epimerase signature. Length = 334 Score = 39.8 bits (93), Expect = 2e-04 Identities = 30/142 (21%), Positives = 45/142 (31%), Gaps = 24/142 (16%) Query: 1681 TVLITGGTGMAGSAVARHVVAR-HGVRNLVLVSRRGPDA------PGAAELVAELAAAGA 1733 L+TG G G V++ ++ H V G D + EL A Sbjct: 2 KYLVTGAAGFIGFHVSKRLLEAGHQV--------VGIDNLNDYYDVSLKQARLELLAQP- 52 Query: 1734 QVQVVACDAADRAALAKVIADIPVQHPLSGVIHTAGALDDAVVMSLTPDRVDVVLRSKVD 1793 Q D ADR + + A + V + L AV SL S + Sbjct: 53 GFQFHKIDLADREGMTDLFASGHFER----VFISPHRL--AVRYSLENPHAYA--DSNLT 104 Query: 1794 AAWHLHELTRDLDVSAFVMFSS 1815 ++ E R + + SS Sbjct: 105 GFLNILEGCRHNKIQHLLYASS 126 Score = 39.8 bits (93), Expect = 2e-04 Identities = 30/142 (21%), Positives = 45/142 (31%), Gaps = 24/142 (16%) Query: 3711 TVLITGGTGMAGSAVARHVVAR-HGVRNLVLVSRRGPDA------PGAAELVAELAAAGA 3763 L+TG G G V++ ++ H V G D + EL A Sbjct: 2 KYLVTGAAGFIGFHVSKRLLEAGHQV--------VGIDNLNDYYDVSLKQARLELLAQP- 52 Query: 3764 QVQVVACDAADRAALAKVIADIPVQHPLSGVIHTAGALDDAVVMSLTPDRVDVVLRSKVD 3823 Q D ADR + + A + V + L AV SL S + Sbjct: 53 GFQFHKIDLADREGMTDLFASGHFER----VFISPHRL--AVRYSLENPHAYA--DSNLT 104 Query: 3824 AAWHLHELTRDLDVSAFVMFSS 3845 ++ E R + + SS Sbjct: 105 GFLNILEGCRHNKIQHLLYASS 126

Rv2209

Rv2216

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
Rv2209	-1	9	0.757401	Probable conserved integral membrane protein
Rv2210c	-2	10	0.924784	Branched-chain amino acid transaminase IlvE
Rv2211c	-2	10	1.222288	Probable aminomethyltransferase GcvT (glycine
Rv2212	-1	11	1.403827	Adenylyl cyclase (ATP pyrophosphate-lyase)
Rv2213	-1	12	1.434063	Probable aminopeptidase PepB
Rv2214c	-1	12	1.378826	Possible short-chain dehydrogenase EphD
Rv2215	-1	12	1.830383	DlaT, dihydrolipoamide acyltransferase, E2
Rv2216	-1	16	0.031372	Conserved protein

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv2209	RTXTOXINA	36	3e-04	Gram-negative bacterial RTX toxin determinant A family...
>RTXTOXINA#Gram-negative bacterial RTX toxin determinant A family signature. Length = 1024 Score = 36.5 bits (84), Expect = 3e-04 Identities = 32/107 (29%), Positives = 45/107 (42%), Gaps = 12/107 (11%) Query: 66 LGNSLSPLI-LQRAGQLRHLLMAAISATAAALVVCNAAVPWTGVGVAAVF-LATTGAGGV 123 +GN L L L G + +SA +A+ ++ NA T AA L T G V Sbjct: 225 VGNKLQNLPNLDNIGAGLDTVSGILSAISASFILSNADAD-TRTKAAAGVELTTKVLGNV 283 Query: 124 VTGVSSVAYTDMISSMLPAVRRGELLLTQGAAGSVLATGVTLVIVPM 170 IS + A R + L T AA ++A+ VTL I P+ Sbjct: 284 ---------GKGISQYIIAQRAAQGLSTSAAAAGLIASAVTLAISPL 321
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv2214c	DHBDHDRGNASE	109	5e-29	2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase signature. Length = 261 Score = 109 bits (274), Expect = 5e-29 Identities = 68/226 (30%), Positives = 107/226 (47%), Gaps = 3/226 (1%) Query: 329 VTGAGSGIGRETALAFAREGAEIVISDIDEATVKDTAAEIAARGGIAYPYVLDVSDAEAV 388 +TGA GIG A A +GA I D + ++ + + A A + DV D+ A+ Sbjct: 13 ITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHAEAFPADVRDSAAI 72 Query: 389 EAFAERVSAEHGVPDIVVNNAGIGQAGRFLDTPAEQFDRVLAVNLGGVVNGCRAFGQRLV 448 + R+ E G DI+VN AG+ + G E+++ +VN GV N R+ + ++ Sbjct: 73 DEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGVFNASRSVSKYMM 132 Query: 449 ERGTGGHIVNVSSMAAYAPLQSLSAYCTSKAATYMFSDCLRAELDAAGVGLTTICPGVID 508 +R G IV V S A P S++AY +SKAA MF+ CL EL + + PG + Sbjct: 133 DR-RSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLELAEYNIRCNIVSPGSTE 191 Query: 509 TNIVATTGFHAPGTDEEKIDGRRGQIDKMFALRSYG-PDKVADAIV 553 T++ + G E+ I G L+ P +ADA++ Sbjct: 192 TDMQWSLWADENG-AEQVIKGSLETFKTGIPLKKLAKPSDIADAVL 236
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv2215	PRTACTNFAMLY	33	0.004	Pertactin virulence factor family signature.
>PRTACTNFAMLY#Pertactin virulence factor family signature. Length = 910 Score = 32.7 bits (74), Expect = 0.004 Identities = 23/56 (41%), Positives = 30/56 (53%), Gaps = 1/56 (1%) Query: 190 GELARIGVAADIGAAPAPKPAPKPVPEPAPTPKAEPAPSPPAAQPAGAAEGAPYVT 245 G+ + +G A PAP+P P+P P P P+A PAP PPA + AA A T Sbjct: 562 GQWSLVGAKAPPAPKPAPQPGPQPPQPPQPQPEA-PAPQPPAGRELSAAANAAVNT 616
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv2216	NUCEPIMERASE	32	0.002	Nucleotide sugar epimerase signature.
>NUCEPIMERASE#Nucleotide sugar epimerase signature. Length = 334 Score = 32.4 bits (74), Expect = 0.002 Identities = 31/146 (21%), Positives = 50/146 (34%), Gaps = 24/146 (16%) Query: 6 VAIAGSSGLIGSALTAALRAADHTVLRI--------VRRAPANSEELHWNPESGEFDPHA 57 + G++G IG ++ L A H V+ I V A E L F H Sbjct: 3 YLVTGAAGFIGFHVSKRLLEAGHQVVGIDNLNDYYDVSLKQARLELL----AQPGFQFHK 58 Query: 58 --LTDVDAVVNLCGVNIAQR----------RWSGAFKQSLRDSRITPTEVLSAAVADAGV 105 L D + + +L +R R+S + DS +T + + Sbjct: 59 IDLADREGMTDLFASGHFERVFISPHRLAVRYSLENPHAYADSNLTGFLNILEGCRHNKI 118 Query: 106 ATLINASAVGYYGNTKDRVVDENDSA 131 L+ AS+ YG + +DS Sbjct: 119 QHLLYASSSSVYGLNRKMPFSTDDSV 144

Rv2504c

Rv2510c

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
Rv2504c	-1	10	0.153472	Probable succinyl-CoA:3-ketoacid-coenzyme A
Rv2505c	-2	10	0.273968	Probable fatty-acid-CoA ligase FadD35
Rv2506	-1	11	0.650171	Probable transcriptional regulatory protein
Rv2507	-1	13	0.599376	Possible conserved proline rich membrane
Rv2508c	0	12	0.241992	Probable conserved integral membrane leucine and
Rv2509	2	13	-0.405815	Probable short-chain type
Rv2510c	2	11	-0.384802	Conserved protein

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv2504c	TYPE3OMGPROT	28	0.047	Type III secretion system outer membrane G protein ...
>TYPE3OMGPROT#Type III secretion system outer membrane G protein family signature. Length = 607 Score = 27.9 bits (62), Expect = 0.047 Identities = 17/68 (25%), Positives = 25/68 (36%), Gaps = 12/68 (17%) Query: 124 GTQVADGGLPWRYDASGGVAVVS-PAKETREFDGVTYVLE-----RGIRTD------FAL 171 + + + WR DAS + VS P + + LE R +T F L Sbjct: 130 RSGIWEPRFGWRPDASNRLVYVSGPPRYLELVEQTAAALEQQTQIRSEKTGALAIEIFPL 189 Query: 172 VHAWQGDR 179 +A DR Sbjct: 190 KYASASDR 197
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv2506	HTHTETR	75	1e-18	TetR bacterial regulatory protein HTH signature.
>HTHTETR#TetR bacterial regulatory protein HTH signature. Length = 215 Score = 74.7 bits (183), Expect = 1e-18 Identities = 38/196 (19%), Positives = 70/196 (35%), Gaps = 9/196 (4%) Query: 17 NRRSQLKSDRRFQLLAAAERLFAERGFLAVRLEDIGAAAGVSGPAIYRHFPNKESLLVEL 76 + Q + R +L A RLF+++G + L +I AAGV+ AIY HF +K L E+ Sbjct: 3 RKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFSEI 62 Query: 77 LVGVSARLLAGARDVT-TRSANLAAALDGLIEFHLDFALGEADLIRIQDRDLAHLPAVAE 135 + + + + + L ++ L+ + E + + V E Sbjct: 63 WELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVTEERRRLLMEIIFHKCEFVGE 122 Query: 136 RQ-VRKAQRQYVEVWVGVLREL------NPGLAEA-DARLMAHAVFGLLNSTPHSMKAAD 187 V++AQR + + L R A + G ++ + A Sbjct: 123 MAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMTRRAAIIMRGYISGLMENWLFAP 182 Query: 188 SKPARTVRARAVLRAM 203 AR + + Sbjct: 183 QSFDLKKEARDYVAIL 198
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv2507	PERTACTIN	30	0.009	Pertactin signature.
>PERTACTIN#Pertactin signature. Length = 922 Score = 30.5 bits (68), Expect = 0.009 Identities = 23/67 (34%), Positives = 24/67 (35%) Query: 53 WSPGGPPPRPPQWPPGPHEASPTQQLPQYWQYDQPPPGGFPPDGLTPPPPQGPRTPRWLW 112 WS G P P P Q PQ Q QPP PP P P R L Sbjct: 560 WSLVGAKAPPAPKPAPQPGPQPGPQPPQPPQPPQPPQPPQPPQRQPEAPAPQPPAGRELS 619 Query: 113 FAAGSAV 119 AA +AV Sbjct: 620 AAANAAV 626
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv2509	DHBDHDRGNASE	76	2e-18	2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase signature. Length = 261 Score = 76.2 bits (187), Expect = 2e-18 Identities = 52/185 (28%), Positives = 89/185 (48%), Gaps = 3/185 (1%) Query: 12 AVVTGASQNIGAALATELAARGHHLIVTARREDVLTELAARLADKYRVTVDVRPADLADP 71 A +TGA+Q IG A+A LA++G H+ + L ++ + L + R + PAD+ D Sbjct: 11 AFITGAAQGIGEAVARTLASQGAHIAAVDYNPEKLEKVVSSLKAEAR-HAEAFPADVRDS 69 Query: 72 QERSKLADELAAR--PISILCANAGTATFGPIASLDLAGEKTQVQLNAVAVHDLTLAVLP 129 ++ + PI IL AG G I SL + +N+ V + + +V Sbjct: 70 AAIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGVFNASRSVSK 129 Query: 130 GMIERKAGGILISGSAAGNSPIPYNATYAATKAFVNTFSESLRGELRGSGVHVTVLAPGP 189 M++R++G I+ GS P A YA++KA F++ L EL + +++PG Sbjct: 130 YMMDRRSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFTKCLGLELAEYNIRCNIVSPGS 189 Query: 190 VRTEL 194 T++ Sbjct: 190 TETDM 194
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv2510c	TONBPROTEIN	31	0.008	Gram-negative bacterial tonB protein signature.
>TONBPROTEIN#Gram-negative bacterial tonB protein signature. Length = 239 Score = 31.1 bits (70), Expect = 0.008 Identities = 21/88 (23%), Positives = 32/88 (36%), Gaps = 2/88 (2%) Query: 425 AIGAAAQASSLQAVYGQTIDRPSAHEILSAKLAPAQEAPAQEAPAP--RGQYDPLPWPDD 482 I A A L Q I+ P+ + +S + + +A P +P P P+ Sbjct: 18 CIHGAVVAGLLYTSVHQVIELPAPAQPISVTMVTPADLEPPQAVQPPPEPVVEPEPEPEP 77 Query: 483 FEVPPMPAPVEPQGPAVWEEILKNPTVK 510 PP APV + P + P K Sbjct: 78 IPEPPKEAPVVIEKPKPKPKPKPKPVKK 105

Rv2586c

Rv2593c

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
Rv2586c	-2	14	1.295413	Probable protein-export membrane protein SecF
Rv2587c	-2	14	1.759725	Probable protein-export membrane protein SecD
Rv2588c	-2	16	2.163827	Probable conserved membrane protein secretion
Rv2589	-3	15	2.335982	4-aminobutyrate aminotransferase GabT
Rv2590	-2	14	2.419034	Probable fatty-acid-CoA ligase FadD9
Rv2591	2	15	3.763267	PE-PGRS family protein PE_PGRS44
Rv2592c	0	12	1.635324	Probable holliday junction DNA helicase RuvB
Rv2593c	1	12	1.248155	Probable holliday junction DNA helicase RuvA

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv2586c	SECFTRNLCASE	250	5e-82	Bacterial translocase SecF protein signature.
>SECFTRNLCASE#Bacterial translocase SecF protein signature. Length = 333 Score = 250 bits (639), Expect = 5e-82 Identities = 75/314 (23%), Positives = 144/314 (45%), Gaps = 28/314 (8%) Query: 58 FEVVGRRRLWFGVSGAIVAVAIASIVFRGFTFGIDFKGGTTVSFPRGSTQVAQVEDVYYR 117 F+ + FG + ++ ++ + G FGIDFKGGTT+ ST V Sbjct: 14 FDFFRWQWATFGAAIVMMIASVILPLVIGLNFGIDFKGGTTIRTE--STTAIDVGVYRAA 71 Query: 118 ALGSEPQSVVIV--------GAGASATVQIRSETLTSD------QTAKLRDALFEAFGPK 163 E V+I A ++I+ + Q +L + + A Sbjct: 72 LEPLELGDVIISEVRDPSFREDQHVAMIRIQMQEDGQGAEGQGAQGQELVNKVETALTAV 131 Query: 164 GTDGQPSKQAISDSAVSETWGGQITKKAVIALVVFLVLVALYITVRYERYMTISAITAML 223 P+ + S +V G++ AV +L+ V++ YI VR+E + A+ A++ Sbjct: 132 D----PALKITSFESVGPKVSGELVWTAVWSLLAATVVIMFYIWVRFEWQFALGAVVALV 187 Query: 224 FDLTVTAGVYSLVGFEVTPATVIGLLTILGFSLYDTVIVFDKVEENTHGFQHTTRRTFAE 283 D+ +T G+++++ + TV LLTI G+S+ DTV+VFD++ EN ++ R + Sbjct: 188 HDVLLTVGLFAVLQLKFDLTTVAALLTITGYSINDTVVVFDRLRENLIKYKTMPLR---D 244 Query: 284 QANLAINQTFMRSINTSLIGVLPVLALMVVAVWLLGVGTLKDLALVQLIGIIIGTYSSIF 343 NL++N+T R++ T + +L ++ +++ G ++ + G+ GTYSS++ Sbjct: 245 VMNLSVNETLSRTVMTGMTTLLALVPMLI-----WGGDVIRGFVFAMVWGVFTGTYSSVY 299 Query: 344 FATPLLVTLRERTE 357 A +++ + Sbjct: 300 VAKNIVLFIGLDRN 313
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv2587c	SECFTRNLCASE	58	3e-11	Bacterial translocase SecF protein signature.
>SECFTRNLCASE#Bacterial translocase SecF protein signature. Length = 333 Score = 57.9 bits (140), Expect = 3e-11 Identities = 41/270 (15%), Positives = 92/270 (34%), Gaps = 20/270 (7%) Query: 279 GMDQRGIGYVVDLQFKGPA--ANIWADYTAAHIGTQTAFTLDSQVVSAPQIQEAIPGGRT 336 G+D +G G + + A +G + Q I Sbjct: 46 GIDFKG-GTTIRTESTTAIDVGVYRAALEPLELGDVIISEVRDPSFREDQHVAMIRIQ-M 103 Query: 337 QISGGDPPFTAATARQLANVLK--YGSLPLSFEPSEAQTVSATLGLSSLRAGMIAGAIGL 394 Q G A ++L N ++ ++ + + + ++V + + + + Sbjct: 104 QEDGQGAEGQGAQGQELVNKVETALTAVDPALKITSFESVGPKVSGELVWTAVWSLLAAT 163 Query: 395 LLVLVY-SLLYYRVLGLLTALSLVASGSMVFAILVLLGRYINYTLDLAGIAGLIIGIGTT 453 ++++ Y + + L ++LV + + +L + L +A L+ G + Sbjct: 164 VVIMFYIWVRFEWQFALGAVVALVHDVLLTVGLFAVLQLKFD----LTTVAALLTITGYS 219 Query: 454 ADSFVVFFERIKDEIR--EGRSFRSAVPRGWARARKTIVSGNAVTFLAAAVLYFLAIGQV 511 + VV F+R+++ + + R + V T LA + + Sbjct: 220 INDTVVVFDRLRENLIKYKTMPLRDVMNLSVNETLSRTVMTGMTTLLALVPMLIWGGDVI 279 Query: 512 KGFAFTLGLTTILDLVVVFLVTWPLVYLAS 541 +GF F + + VF T+ VY+A Sbjct: 280 RGFVFAM-------VWGVFTGTYSSVYVAK 302 Score = 36.4 bits (84), Expect = 2e-04 Identities = 17/100 (17%), Positives = 35/100 (35%), Gaps = 8/100 (8%) Query: 13 YLSVFLVMLIGIYLLVFFTGDKHTAPKLGIDLQGGTRVTLTARTPDGSAPSREALAQAQQ 72 + + ++M+ + L + GID +GGT + + T R AL + + Sbjct: 24 FGAAIVMMIASVILPLVI------GLNFGIDFKGGTTIRTESTTAIDVGVYRAAL-EPLE 76 Query: 73 IISARVNGLGVSGSEVVVDGDNLVITVPGNDGSEARNLGQ 112 + ++ + S ++ DG A G Sbjct: 77 LGDVIISEVR-DPSFREDQHVAMIRIQMQEDGQGAEGQGA 115
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv2590	NUCEPIMERASE	44	3e-06	Nucleotide sugar epimerase signature.
>NUCEPIMERASE#Nucleotide sugar epimerase signature. Length = 334 Score = 43.6 bits (103), Expect = 3e-06 Identities = 53/246 (21%), Positives = 82/246 (33%), Gaps = 65/246 (26%) Query: 770 TVLLTGATGFLGRYLALEWLDRMDLVNGKLICLVRARSDEEAQARLDATFDSGDPYLVRH 829 L+TGA GF+G +++ L+ V G +D D D L + Sbjct: 2 KYLVTGAAGFIGFHVSKRLLEAGHQVVG-----------------IDNLNDYYDVSLKQA 44 Query: 830 YRE-LGAGRLEVLAGDKGEADL--------GLDRVTWQRLADTV-DLIVDPAALVNHVLP 879 E L + D + + +RV V + +P A + Sbjct: 45 RLELLAQPGFQFHKIDLADREGMTDLFASGHFERVFISPHRLAVRYSLENPHAYAD---- 100 Query: 880 YSQLFGPNAAGTAELLRLALTGKRKPYIYTSTIAV---GEQIPPEAFTEDADI-RAISPT 935 N G +L K + +Y S+ +V ++P F+ D + +S Sbjct: 101 ------SNLTGFLNILEGCRHNKIQHLLYASSSSVYGLNRKMP---FSTDDSVDHPVSL- 150 Query: 936 RRIDDSYANGYANSKWAGEVLLREAHEQCGLPVTVFRCDMILADTSYTGQLNLPDM---- 991 YA +K A E++ GLP T R T Y G PDM Sbjct: 151 ----------YAATKKANELMAHTYSHLYGLPATGLR-----FFTVY-GPWGRPDMALFK 194 Query: 992 FTRLML 997 FT+ ML Sbjct: 195 FTKAML 200
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv2591	cloacin	37	2e-04	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 37.0 bits (85), Expect = 2e-04 Identities = 31/93 (33%), Positives = 40/93 (43%), Gaps = 3/93 (3%) Query: 124 GANGTAASPNGGDGGILYGNGGN---GFSQTTAGVAGGAGGSAGLIGNGGNGGAGGAGAA 180 GA+ T+ + NGG G+ G G + G+S GG+G G G+G GG G + Sbjct: 12 GAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNGNS 71 Query: 181 GGAGGAGGWLLGNGGAGGPGGPTDVPAGTGGAG 213 GG G GG L G P G GG Sbjct: 72 GGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 34.7 bits (79), Expect = 9e-04 Identities = 29/102 (28%), Positives = 34/102 (33%) Query: 173 GAGGAGAAGGAGGAGGWLLGNGGAGGPGGPTDVPAGTGGAGGAGGDAPLIGWGGNGGPGG 232 G G G GA G + G G GG +G G G GG G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 233 FAAFGNGGAGGNGGASGSLFGVGGAGGVGGSSEDVGGTGGAG 274 GNG +GG G G+L V G + G GG Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 32.8 bits (74), Expect = 0.004 Identities = 36/104 (34%), Positives = 43/104 (41%), Gaps = 8/104 (7%) Query: 361 TGGDGGLGGAGAVLIGTGV-GGFGGLGGGSNGTGGAGGA------GGTGATLIGLGAGGG 413 +GGDG GA + GG GLG G + G+G + GG + I G G G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 414 GGIGGFAVNVGNGVGGLGGQGGQGAAL-IGLGAGGAGGAGGATV 456 G GG N G G G G A + G A GAGG V Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAV 105 Score = 32.8 bits (74), Expect = 0.004 Identities = 34/113 (30%), Positives = 42/113 (37%), Gaps = 10/113 (8%) Query: 316 GDGGNGGAGTAIGSNAGDGGAGGDSSALIGYAQGGSGGLGGFGESTGGDGGLGGAGAVLI 375 G G N GA + G+ G G GG+ G+ GG G+G Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVG--------GGASDGSGWSSENNPWGGGSGSGIHWG 57 Query: 376 GTGVGGFGGLGGGSNGTGGAGGAGGTGATLIGLG--AGGGGGIGGFAVNVGNG 426 G G GG G S G G GG A + G A G GG AV++ G Sbjct: 58 GGSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAG 110 Score = 32.4 bits (73), Expect = 0.005 Identities = 29/103 (28%), Positives = 41/103 (39%) Query: 298 GGDGGAGGTAGGRLFSLGGDGGNGGAGTAIGSNAGDGGAGGDSSALIGYAQGGSGGLGGF 357 G + GA T+G G G GGA G ++ + GG S + I + G G GG Sbjct: 8 GHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGG 67 Query: 358 GESTGGDGGLGGAGAVLIGTGVGGFGGLGGGSNGTGGAGGAGG 400 ++GG G GG + + GF L G + G Sbjct: 68 NGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAG 110 Score = 30.8 bits (69), Expect = 0.015 Identities = 38/139 (27%), Positives = 50/139 (35%), Gaps = 9/139 (6%) Query: 223 GWGGNGGPGGFAAFGNGGAGGNGGASGSLFGVGGAGGVGGSSEDVGGTGGAGGAGRGLFL 282 G G N G + NGG G G G + G G SSE+ GG+G Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGG------ASDGSGWSSENNPWGGGSGSGIHWGGG 59 Query: 283 GLGGDGGAGGTSNNNGGDGGAGGTAGGRL---FSLGGDGGNGGAGTAIGSNAGDGGAGGD 339 G+GG G S G GG + F G GG +I + A Sbjct: 60 SGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAAIADI 119 Query: 340 SSALIGYAQGGSGGLGGFG 358 +AL G + G G+ +G Sbjct: 120 MAALKGPFKFGLWGVALYG 138 Score = 30.5 bits (68), Expect = 0.022 Identities = 33/116 (28%), Positives = 37/116 (31%), Gaps = 10/116 (8%) Query: 283 GLGGDGGAGGTSNNNGGDGGAGGTAGGRLFSLGGDGGNGGAGTAIGSNAGDGGAGGDSSA 342 G G + GA TS N G G GG G N G GS GG G Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG---- 61 Query: 343 LIGYAQGGSGGLGGFGESTGGDGGLGGAGAVLIGTGVGGFGGLGGGSNGTGGAGGA 398 G+GG G G GG A A + G G G + GA Sbjct: 62 ------HGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGA 111 Score = 29.3 bits (65), Expect = 0.049 Identities = 29/88 (32%), Positives = 33/88 (37%), Gaps = 11/88 (12%) Query: 444 GAGGAGGAGGATVVGLGGNGGDGGDGGGLFSIGVGGDGGNAGNGAMPANGGNGGNAGVIA 503 G G G GA NGG G G G G DG + P GG+G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVG----GGASDGSGWSSENNPWGGGSGSGIHWGG 58 Query: 504 NGSFAPSFVGFGGNGGNGVNGGTGGSGG 531 G G GGNG +GG G+GG Sbjct: 59 GS-------GHGNGGGNGNSGGGSGTGG 79
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv2593c	DPTHRIATOXIN	30	0.006	Diphtheria toxin signature.
>DPTHRIATOXIN#Diphtheria toxin signature. Length = 567 Score = 30.1 bits (67), Expect = 0.006 Identities = 37/123 (30%), Positives = 53/123 (43%), Gaps = 18/123 (14%) Query: 74 LLSVSGVGPRLA---MAALAVHDAPALRQVLADG---NVAALTRVPGIGKRGAERMVLEL 127 L +V+G P A AA AV+ A + AD AAL+ +PGIG V+ + Sbjct: 295 LKTVTGTNPVFAGANYAAWAVNVAQVIDSETADNLEKTTAALSILPGIGS------VMGI 348 Query: 128 RDKV------GVAATGGALSTNGHAVRSPVVEALVGLGFAAKQAEEATDTVLAANHDATT 181 D + A ALS+ A P+V LV +GFAA E+ + H++ Sbjct: 349 ADGAVHHNTEEIVAQSIALSSLMVAQAIPLVGELVDIGFAAYNFVESIINLFQVVHNSYN 408 Query: 182 SSA 184 A Sbjct: 409 RPA 411

Rv2737A

Rv2747

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
Rv2737A	3	12	5.463400	Conserved hypothetical cysteine rich protein
Rv2738c	3	11	5.225851	Conserved hypothetical protein
Rv2739c	2	11	4.235482	Possible alanine rich transferase
Rv2740	4	12	3.255539	Epoxide hydrolase
Rv2741	4	13	2.706810	PE-PGRS family protein PE_PGRS47
Rv2742c	1	17	-0.646765	Conserved hypothetical arginine rich protein
Rv2743c	0	8	0.232447	Possible conserved transmembrane alanine rich
Rv2744c	1	10	-0.102681	Conserved 35 kDa alanine rich protein
Rv2745c	0	12	0.277098	Transcriptional regulatory protein ClgR
Rv2746c	-1	12	-0.173550	Probable PGP synthase PgsA3
Rv2747	0	13	-0.335289	Probable L-glutamate alpha-N-acetyltranferase

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv2737A	2FE2SRDCTASE	38	4e-07	Ferric iron reductase signature.
>2FE2SRDCTASE#Ferric iron reductase signature. Length = 262 Score = 37.7 bits (87), Expect = 4e-07 Identities = 13/26 (50%), Positives = 16/26 (61%) Query: 32 DLTFRRRSCCLFYRVPAGGKCGDCPL 57 D RR+CC YR+P +CGDC L Sbjct: 236 DGLLVRRTCCQRYRLPDVQQCGDCTL 261
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv2741	cloacin	39	3e-05	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 39.3 bits (91), Expect = 3e-05 Identities = 37/113 (32%), Positives = 48/113 (42%), Gaps = 5/113 (4%) Query: 233 GVGGAALGGGAQAAGGNGGAGGVGGLFGAGGAGGAGGFSDTGGTGGAGGAGGLFGPGGGS 292 G G GA + GN G G G G + G+G S+ GG G+G +G G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 293 GGVGGFGDTGGTGGDGGSGGLFGVGGAGGHGGFGSAAGGDGGAGGAGGTVFGS 345 G GG G++GG G GG+ A FG A GAGG ++ Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVA-----FGFPALSTPGAGGLAVSISAG 110 Score = 35.5 bits (81), Expect = 6e-04 Identities = 29/78 (37%), Positives = 32/78 (41%), Gaps = 1/78 (1%) Query: 289 GGGSGGVGGFGDTGGTGGDGGSGGLFGVGGAGGHGGFGSAAGGDGGAGGAGGTVFGSGGA 348 G G G G T G +GG GL GGA G+ S GG G+G G G Sbjct: 4 GDGRGHNTGAHSTSGNI-NGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 349 GGAGGVATVAGHGGHGGN 366 G GG G G GGN Sbjct: 63 GNGGGNGNSGGGSGTGGN 80 Score = 34.3 bits (78), Expect = 0.001 Identities = 30/89 (33%), Positives = 36/89 (40%), Gaps = 1/89 (1%) Query: 372 GTGGAGGAGGFGGFGGDGGDGGIGGLVGSGGAGGSGGTGTLSGGRGGAGGNAGTFYGSG- 430 G G G G G+ G G VG G + GSG + + GG+G GSG Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 431 GAGGAGGESDNGDGGNGGVGGKAGLVGEG 459 G GG G S G G G + A V G Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFG 91 Score = 33.9 bits (77), Expect = 0.002 Identities = 38/115 (33%), Positives = 43/115 (37%), Gaps = 5/115 (4%) Query: 400 SGGAGGSGGTGTLSGGRGGAGGNAGTFYGSGGAGGAG-GESDNGDGGNGGVGGKAGLVGE 458 SGG G TG S GG G G G + G+G +N GG G G G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 459 GGNGGDGGATIAGKGGSGGNGGNAWLTGQGGNGGNAAFGKAGTGSVGVGGAGGLL 513 GNGG G GG G GGN G A G G + V + G L Sbjct: 62 HGNGGGNG----NSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGAL 112 Score = 33.5 bits (76), Expect = 0.002 Identities = 28/102 (27%), Positives = 37/102 (36%), Gaps = 2/102 (1%) Query: 260 GAGGAGGAGGFSDTGGTGGAGGAGGLFGPGGGSGGVGGFGDTGGTGGDGGSGGLFGVGGA 319 G G G G T G G G G GGG+ G+ G G G+ GG+ Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGL--GVGGGASDGSGWSSENNPWGGGSGSGIHWGGGS 60 Query: 320 GGHGGFGSAAGGDGGAGGAGGTVFGSGGAGGAGGVATVAGHG 361 G G G+ G G G + + A G ++T G Sbjct: 61 GHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG 102 Score = 33.5 bits (76), Expect = 0.002 Identities = 33/113 (29%), Positives = 41/113 (36%), Gaps = 5/113 (4%) Query: 160 GAAGLFGNGGAGGAGASNQAGNGGAGGNGGAG-GLIWGTAGTGGNGGFTTFLDAAGGAGG 218 G G N GA + G G G GGA G W + GG + + GG+G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 219 AGGAGGLFGAGGAGGVGGAALGGGAQAAG----GNGGAGGVGGLFGAGGAGGA 267 G G GG+G G + A G GAGG+ AG A Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAA 115 Score = 33.5 bits (76), Expect = 0.002 Identities = 21/72 (29%), Positives = 28/72 (38%) Query: 120 NGANGKPGTGQDGGAGGLLYGSGGNGGSGLAGSGQKGGNGGAAGLFGNGGAGGAGASNQA 179 N +GG GL G G + GSG + G G +G+ GG+G Sbjct: 10 NTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNG 69 Query: 180 GNGGAGGNGGAG 191 +GG G GG Sbjct: 70 NSGGGSGTGGNL 81 Score = 32.8 bits (74), Expect = 0.004 Identities = 35/105 (33%), Positives = 42/105 (40%), Gaps = 5/105 (4%) Query: 169 GAGGAGASNQAGNGGAGGNGGAGGLIWGTAGTGGNGGFTTFLDAAGGAGGAGGAGGLFGA 228 G G G + A + NGG GL G + G+G + GG+G GG G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 229 GGAGGVGGAALGGGAQAAGGNGGAGGVGGLFG--AGGAGGAGGFS 271 G GG G GG GGN A FG A GAGG + Sbjct: 63 GNGGGNGN---SGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 32.0 bits (72), Expect = 0.006 Identities = 30/103 (29%), Positives = 37/103 (35%), Gaps = 1/103 (0%) Query: 198 AGTGGNGGFTTFLDAAGGAGGAGGAGGLFGAGGAGGVGGAALGGGAQAAGGNGGAGGVGG 257 +G G G T +G G G+ G G + +G GG G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 258 LFGAGGAGGAGGFSDTGGTGGAGGAGGLFG-PGGGSGGVGGFG 299 GG G +GG S TGG A A FG P + G GG Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 32.0 bits (72), Expect = 0.006 Identities = 33/108 (30%), Positives = 41/108 (37%), Gaps = 5/108 (4%) Query: 326 GSAAGGDGGAGGAGGTVFGSGGAGGAGGVATVAGHGGHGGNAGLLYGTGGAGGAGGFGGF 385 G G + GA G + G G GG A+ G G N G+G GG G Sbjct: 4 GDGRGHNTGAHSTSGNINGGPTGLGVGGGAS-DGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 386 GGDGGDGGIGGLVGSGGAGGSGGTGTLSG----GRGGAGGNAGTFYGS 429 G GG+G GG G+GG + G GAGG A + Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAG 110 Score = 32.0 bits (72), Expect = 0.007 Identities = 32/112 (28%), Positives = 38/112 (33%), Gaps = 7/112 (6%) Query: 140 GSGGNGGSGLAGSGQKGGNGGAAGLFGNGGAGGAGASNQAGNGGAGGNGGAGGLIWGTAG 199 G G N G+ GG G G G + N GG+G GG G Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH--G 63 Query: 200 TGGNGGFTTFLDAAGGAGGAGGAGGLFG-----AGGAGGVGGAALGGGAQAA 246 GG G + GG A A FG GAGG+ + G AA Sbjct: 64 NGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAA 115 Score = 31.6 bits (71), Expect = 0.009 Identities = 29/103 (28%), Positives = 36/103 (34%), Gaps = 1/103 (0%) Query: 318 GAGGHGGFGSAAGGDGGAGGAGGTVFGSGGAGGAGGVATVAGHGGHGGNAGLLYGTGGAG 377 G G G A G G + GGA G ++ G G +G+ +G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 378 GAGGFGGFGGDGGDGGIGGLVGSGGAGGSGGTGTLSGGRGGAG 420 G GG G G GG G G L G + G GG Sbjct: 63 GNGGGNGNSG-GGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv2742c	GALLIDERMIN	30	0.001	Gallidermin signature.
>GALLIDERMIN#Gallidermin signature. Length = 52 Score = 30.1 bits (67), Expect = 0.001 Identities = 10/21 (47%), Positives = 13/21 (61%) Query: 242 NAICAIEDGVEPRVAWWALCT 262 NA + + G EPR+A LCT Sbjct: 18 NAKESNDSGAEPRIASKFLCT 38
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv2744c	IGASERPTASE	28	0.031	IgA-specific serine endopeptidase (S6) signature.
>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature. Length = 1541 Score = 28.5 bits (63), Expect = 0.031 Identities = 33/228 (14%), Positives = 68/228 (29%), Gaps = 15/228 (6%) Query: 24 ADPKVQIQQAIEEAQRTHQALTQQA--AQVIGNQRQLEMRLNRQLADIEKLQVNVRQALT 81 + Q ++ +EA+ +A TQ AQ ++ + ++ A +EK + + + Sbjct: 1059 TETTAQNREVAKEAKSNVKANTQTNEVAQSGSETKETQTTETKETATVEKEE---KAKVE 1115 Query: 82 LADQATAAGDAAKATEYNNAAEAF--AAQLVTAEQSVEDLKTLHDQALSAAAQAKKAVER 139 ++ + +E A+ ++K Q + A + A E Sbjct: 1116 TEKTQEVPKVTSQVSPKQEQSETVQPQAEPARENDPTVNIKEPQSQTNTTADTEQPAKET 1175 Query: 140 NAMVLQQKIAERTKLLSQLEQAKMQEQVSASLRSMSELAAPGNTPSLDEVRDKIERRYAN 199 ++ V + V + P RR Sbjct: 1176 SSNV-------EQPVTESTTVNTGNSVVENPENTTPATTQPTVNSESSNKPKNRHRRSVR 1228 Query: 200 AI-GSAELAESSVQGRMLEVEQAGIQMAGHSRLEQIRASMRGEALPAG 246 ++ + E A +S R ++ L RA + AL G Sbjct: 1229 SVPHNVEPATTSSNDRSTVALCDLTSTNTNAVLSDARAKAQFVALNVG 1276
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv2747	SACTRNSFRASE	40	8e-07	Streptothricin acetyltransferase signature.
>SACTRNSFRASE#Streptothricin acetyltransferase signature. Length = 173 Score = 40.3 bits (94), Expect = 8e-07 Identities = 14/73 (19%), Positives = 31/73 (42%), Gaps = 5/73 (6%) Query: 61 KVVGCGALHVLWSDLGEIRTVAVDPAMTGHGIGHAIVDRLLQVARDLQLQRVFVLTFET- 119 +G + W+ I +AV G+G A++ + ++ A++ + + T + Sbjct: 75 NCIGRIKIRSNWNGYALIEDIAVAKDYRKKGVGTALLHKAIEWAKENHFCGLMLETQDIN 134 Query: 120 ----EFFARHGFT 128 F+A+H F Sbjct: 135 ISACHFYAKHHFI 147

Rv2843

Rv2853

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
Rv2843	-2	8	0.755173	Probable conserved transmembrane alanine rich
Rv2844	-2	9	0.414762	Conserved alanine rich protein
Rv2845c	-2	8	1.183822	Probable prolyl-tRNA synthetase ProS
Rv2846c	-1	8	1.383191	Possible integral membrane efflux protein EfpA
Rv2847c	-3	8	1.166179	Possible multifunctional enzyme siroheme
Rv2848c	0	11	2.876429	Probable cobyrinic acid A,C-diamide synthase
Rv2849c	0	11	2.468816	Probable cob(I)alamin adenosyltransferase CobO
Rv2850c	-1	10	1.743389	Possible magnesium chelatase
Rv2851c	0	13	0.360859	GCN5-related N-acetyltransferase
Rv2852c	0	12	0.141826	Probable malate:quinone oxidoreductase Mqo
Rv2853	1	11	0.650048	PE-PGRS family protein PE_PGRS48

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv2843	PYOCINKILLER	28	0.025	Pyocin S killer protein signature.
>PYOCINKILLER#Pyocin S killer protein signature. Length = 617 Score = 27.8 bits (61), Expect = 0.025 Identities = 24/120 (20%), Positives = 44/120 (36%), Gaps = 13/120 (10%) Query: 56 QARHDGALAAAAATAIGIPPQVAAALTVVATQRTSHARALATEIARAAGKLVSATSETSS 115 Q R + AA A+ AAA Q + A+ A E AR + +A + Sbjct: 201 QIRMNTLTAAKASIE-------AAAANKAREQAAAEAKRKAEEQARQQAAIRAANTYAMP 253 Query: 116 SSPSPTDPAAPPPAV------SDVIDSLRTSAGEASRLVATTSGYRAGLLASIAASCTAS 169 ++ S AA + + + ++ + R++A+ A AS+ S + Sbjct: 254 ANGSVVATAAGRGLIQVAQGAASLAQAISDAIAVLGRVLASAPSVMAVGFASLTYSSRTA 313
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv2846c	TCRTETB	113	3e-29	Tetracycline resistance protein TetB signature.
>TCRTETB#Tetracycline resistance protein TetB signature. Length = 458 Score = 113 bits (285), Expect = 3e-29 Identities = 79/368 (21%), Positives = 152/368 (41%), Gaps = 19/368 (5%) Query: 61 MQLLATMDSTVAIVALPKIQNELSLSDAGRSWVITAYVLTFGGLMLLGGRLGDTIGRKRT 120 + + ++ V V+LP I N+ + A +WV TA++LTF + G+L D +G KR Sbjct: 22 LSFFSVLNEMVLNVSLPDIANDFNKPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRL 81 Query: 121 FIVGVALFTISSVLCAVAWDEATLVI-ARLSQGVGSAIASPTGLALVATTFPKGPARNAA 179 + G+ + SV+ V +L+I AR QG G+A A P + +V + R A Sbjct: 82 LLFGIIINCFGSVIGFVGHSFFSLLIMARFIQGAGAA-AFPALVMVVVARYIPKENRGKA 140 Query: 180 TAVFAAMTAIGSVMGLVVGGALTEVSWRWAFLVNVP-IGLVMIYLARTALRETNKERMKL 238 + ++ A+G +G +GG + W++L+ +P I ++ + L++ + + Sbjct: 141 FGLIGSIVAMGEGVGPAIGGMIAH-YIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHF 199 Query: 239 DATGAILATLACTAAVFAFSIGPEKGWMSGITIGSGLVALAAAVAFVIVERTAENPVVPF 298 D G IL ++ + F+ ++ + + FV R +P V Sbjct: 200 DIKGIILMSVGIVFFML-FTTSYSISFLIVSVLSFLI--------FVKHIRKVTDPFVDP 250 Query: 299 HLFRDRNRLVTFSAILLAGGVMFSLTVCIGLYVQDILGYSALRAGVGFI-PFVIAMGIGL 357 L ++ ++ + G + + ++D+ S G I P +++ I Sbjct: 251 GLGKNIPFMIGVLCGGIIFGTVAGFVSMVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFG 310 Query: 358 GVSSQLVSRFSPRVLTIGGGYLLFGAMLYGSFFMHRGVPYFPNLVMPIVVGGIGIGMAVV 417 + LV R P + G L + L SF + M I++ + G++ Sbjct: 311 YIGGILVDRRGPLYVLNIGVTFLSVSFLTASFL-----LETTSWFMTIIIVFVLGGLSFT 365 Query: 418 PLTLSAIA 425 +S I Sbjct: 366 KTVISTIV 373
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv2848c	DHBDHDRGNASE	37	1e-04	2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase signature. Length = 261 Score = 36.6 bits (84), Expect = 1e-04 Identities = 19/88 (21%), Positives = 32/88 (36%), Gaps = 3/88 (3%) Query: 256 VAIAAGRAFTFGYAEHAEMLRAAGAEVVEFDPLSETLPEGTDAVVLPGGFPEQFTAELSA 315 +A G A G A A L + GA + D E L + ++ E F A++ Sbjct: 10 IAFITGAAQGIGEAV-ARTLASQGAHIAAVDYNPEKLEKVVSSLKAEARHAEAFPADVRD 68 Query: 316 NDTVRRQINELAAAGAPVHA--ECAGLL 341 + + + P+ AG+L Sbjct: 69 SAAIDEITARIEREMGPIDILVNVAGVL 96
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv2850c	TONBPROTEIN	34	0.001	Gram-negative bacterial tonB protein signature.
>TONBPROTEIN#Gram-negative bacterial tonB protein signature. Length = 239 Score = 33.8 bits (77), Expect = 0.001 Identities = 26/164 (15%), Positives = 47/164 (28%), Gaps = 18/164 (10%) Query: 300 DEALALASVDPEPEPDPPGGGQSANEPASQPNSRSKSTEPGAPSSMGDDPPRPASPRLRS 359 + +++ V P P A QP P + + P + Sbjct: 42 AQPISVTMVTPADLEPPQ---------AVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKP 92 Query: 360 SPRPSAPPSKIFRTRALRVPGVGTGAPGRRSRARNASGSVVAAAEVSDPDAHGLHLFATL 419 P+P P + + + V S N + + + ++ + + + A+ Sbjct: 93 KPKPKPKPKPVKKVQEQPKRDVKPVESRPASPFENTAPARLTSSTATAATSKPVTSVASG 152 Query: 420 LAAGERAFGAGPLRPWPDDVRRAIREGREGN-LVIFVVDASGSM 462 A R P RA EG V F V G + Sbjct: 153 PRALSRNQPQYP--------ARAQALRIEGQVKVKFDVTPDGRV 188
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv2853	cloacin	40	2e-05	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 40.1 bits (93), Expect = 2e-05 Identities = 27/81 (33%), Positives = 34/81 (41%) Query: 348 GAGGNGGKGGFAQATTSVTGGNGGNGGNGHDSNAPGGAGGSGGVGGDGGRGGLLAGNGGT 407 G G G G + ++ GG G G G S+ G + + GG G G G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 408 GGAGGNGGTGGAGAPGGAGGA 428 G GGNG +GG GG A Sbjct: 63 GNGGGNGNSGGGSGTGGNLSA 83 Score = 38.2 bits (88), Expect = 1e-04 Identities = 30/87 (34%), Positives = 34/87 (39%), Gaps = 1/87 (1%) Query: 525 GGDGGAGGTGGNGGDGGAGGAPGLGGAGGAGGWLIGQSGSTGGGGAGGAGGAGGAGGAGG 584 G + GA T GN G G G GGA GW + GG G+G G G G GG Sbjct: 8 GHNTGAHSTSGNINGGPTGLGVG-GGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGG 66 Query: 585 SGGAGGHGDTTSGKNGSSGTAGFDGNP 611 G G G T G + G P Sbjct: 67 GNGNSGGGSGTGGNLSAVAAPVAFGFP 93 Score = 37.4 bits (86), Expect = 2e-04 Identities = 30/91 (32%), Positives = 40/91 (43%) Query: 259 NGGNTDLTGGAGGDGNAGSTTVNGGNGGTGGAARNSSGGTGNSFGGAGGAGGDGANGGDG 318 NGG T L G G +G ++ N GG G+ + GG+G+ GG G G G+ G Sbjct: 21 NGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNGNSGGGSGTGGN 80 Query: 319 GAGGEALTEGGATAVSGAGGKGGNAEASGGA 349 + A G A+S G G S GA Sbjct: 81 LSAVAAPVAFGFPALSTPGAGGLAVSISAGA 111 Score = 36.6 bits (84), Expect = 3e-04 Identities = 31/93 (33%), Positives = 38/93 (40%) Query: 439 GDNATVTGGNGGTGGDGGSALGTGGAGGAGGLGGHGGAGGLLIGNGGAGGAGGLGGAGGA 498 G ++T NGG G G + G+G + GG G I GG G G GG G + Sbjct: 12 GAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNGNS 71 Query: 499 GGAGGEGGAGGAGGEAIPGGASTNSAGGDGGAG 531 GG G GG A + G S G GG Sbjct: 72 GGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 36.6 bits (84), Expect = 3e-04 Identities = 34/125 (27%), Positives = 41/125 (32%), Gaps = 9/125 (7%) Query: 482 GNGGAGGAGGLGGAGGAGGAGGEGGAGGAGGEAIPGGASTNSAGGDGGAGGTGGNGGDGG 541 G G G G G G G G G G +S N+ G G G GG G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 542 AGGAPGLGGAGGAGGWLIGQSGSTGGGGAGGAGGAGGAGGAGGSGGAGGHGDTTSGKNGS 601 G GG+G TGG + A A + GAGG + S S Sbjct: 63 GNGGGNGNSGGGSG---------TGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALS 113 Query: 602 SGTAG 606 + A Sbjct: 114 AAIAD 118 Score = 34.3 bits (78), Expect = 0.002 Identities = 28/86 (32%), Positives = 36/86 (41%) Query: 461 TGGAGGAGGLGGHGGAGGLLIGNGGAGGAGGLGGAGGAGGAGGEGGAGGAGGEAIPGGAS 520 +GG G G H +G + G G G GG G G G G GG+ Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 521 TNSAGGDGGAGGTGGNGGDGGAGGAP 546 + GG+G +GG G GG+ A AP Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAAP 87 Score = 33.5 bits (76), Expect = 0.002 Identities = 27/93 (29%), Positives = 35/93 (37%) Query: 140 NGGNGAAGGPNQAGGAGGNAGLIGNGGAGGAGGVGAVGGKRGTGGLLFGNGGAGGQGGLG 199 N G + G G G G + G+G + GG G+G G G G GG G Sbjct: 10 NTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNG 69 Query: 200 LAGINGGSGGQGGHGGNAILFGQGGAGGPGGTG 232 +G G+GG + FG PG G Sbjct: 70 NSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG 102 Score = 32.4 bits (73), Expect = 0.006 Identities = 30/95 (31%), Positives = 43/95 (45%), Gaps = 1/95 (1%) Query: 258 GNGGNTDLTGGAGGDGNAGSTTVNGGNGGTGGAARNSSGGTGNSFGGAGGAGGDGANGGD 317 G+ T G G G G + G+G ++ N+ G G+ G G G NGG Sbjct: 8 GHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGG 67 Query: 318 GG-AGGEALTEGGATAVSGAGGKGGNAEASGGAGG 351 G +GG + T G +AV+ G A ++ GAGG Sbjct: 68 NGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG 102 Score = 32.0 bits (72), Expect = 0.008 Identities = 30/93 (32%), Positives = 36/93 (38%), Gaps = 7/93 (7%) Query: 269 AGGDGNAGSTTVNGGNGGTGGAARNSSGGTGNSFGGAGGAGGDGANGGDGGAGGEALTEG 328 +GGDG N G T G G G G + G+G N GG G + G Sbjct: 2 SGGDGRGH----NTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWG 57 Query: 329 GATAVSGAGGKGGNAEASGGAGGNGGKGGFAQA 361 G SG G GGN + GG+G G A Sbjct: 58 G---GSGHGNGGGNGNSGGGSGTGGNLSAVAAP 87 Score = 29.7 bits (66), Expect = 0.038 Identities = 31/110 (28%), Positives = 40/110 (36%), Gaps = 1/110 (0%) Query: 371 GNGGNGHDSNAPGGAGG-SGGVGGDGGRGGLLAGNGGTGGAGGNGGTGGAGAPGGAGGAG 429 G G GH++ A +G +GG G G GG G+G + GG G+G G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 430 GKADIANSLGDNATVTGGNGGTGGDGGSALGTGGAGGAGGLGGHGGAGGL 479 G + G + G GAGGL AG L Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGAL 112 Score = 29.7 bits (66), Expect = 0.039 Identities = 25/68 (36%), Positives = 32/68 (47%), Gaps = 4/68 (5%) Query: 121 GADGSTPGQAGGPGGLLYGNGGNGAAGGPNQ----AGGAGGNAGLIGNGGAGGAGGVGAV 176 GA ++ GGP GL G G + +G ++ GG+G G G G GG G Sbjct: 12 GAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNGNS 71 Query: 177 GGKRGTGG 184 GG GTGG Sbjct: 72 GGGSGTGG 79

Rv2905

Rv2914c

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
Rv2905	-1	14	-1.652002	Probable conserved alanine rich lipoprotein
Rv2906c	-1	14	-0.909354	Probable tRNA (guanine-N1)-methyltransferase
Rv2907c	-1	13	-1.185697	Probable 16S rRNA processing protein RimM
Rv2908c	-1	10	-0.928422	Conserved hypothetical protein
Rv2909c	-1	8	-0.181904	30S ribosomal protein S16 RpsP
Rv2910c	-2	7	0.255539	Conserved hypothetical protein
Rv2911	-2	8	0.717903	Probable penicillin-binding protein DacB2
Rv2912c	-1	7	0.666713	Probable transcriptional regulatory protein
Rv2913c	-2	6	0.807283	Possible D-amino acid aminohydrolase (D-amino
Rv2914c	-2	7	0.978406	Probable transmembrane serine/threonine-protein

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv2905	BLACTAMASEA	29	0.022	Beta-lactamase class A signature.
>BLACTAMASEA#Beta-lactamase class A signature. Length = 286 Score = 29.0 bits (65), Expect = 0.022 Identities = 31/156 (19%), Positives = 50/156 (32%), Gaps = 36/156 (23%) Query: 76 PTRVRQATDEAAAMGATLSVAVLDRATGQLVSNGNT-QIIATASVAKLFIADDLLLAEAE 134 P + Q + + + + +D A+G+ ++ + S K+ + +L Sbjct: 23 PQPLEQIKLSESQLSGRVGMIEMDLASGRTLTAWRADERFPMMSTFKVVLCGAVLARVDA 82 Query: 135 GK--------------VTLSP--EDHHA--------LDVMLQSSDDGAAERFWSQDGGNA 170 G V SP E H A + SD+ AA + GG A Sbjct: 83 GDEQLERKIHYRQQDLVDYSPVSEKHLADGMTVGELCAAAITMSDNSAANLLLATVGGPA 142 Query: 171 VVTQVARRYGLRST-----------APPSDGRWWNT 195 +T R+ G T A P D R T Sbjct: 143 GLTAFLRQIGDNVTRLDRWETELNEALPGDARDTTT 178
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv2909c	IGASERPTASE	32	7e-04	IgA-specific serine endopeptidase (S6) signature.
>IGASERPTASE#IgA-specific serine endopeptidase (S6) signature. Length = 1541 Score = 32.3 bits (73), Expect = 7e-04 Identities = 11/46 (23%), Positives = 14/46 (30%) Query: 117 PTTEATKPKKKSPAKKAAKAAEPAPQPEQPDTPALGGEQAELTAES 162 T + S + A P P PA E E AE+ Sbjct: 998 TTPNNIQADVPSVPSNNEEIARVDEAPVPPPAPATPSETTETVAEN 1043
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv2911	BLACTAMASEA	33	9e-04	Beta-lactamase class A signature.
>BLACTAMASEA#Beta-lactamase class A signature. Length = 286 Score = 33.2 bits (76), Expect = 9e-04 Identities = 29/116 (25%), Positives = 40/116 (34%), Gaps = 9/116 (7%) Query: 49 DLDSGQVLAGRDQNVAHPPASTIKVLLALVALDELD-----LNSTVVADVADTQAECNCV 103 DL SG+ L + P ST KV+L L +D L + D Sbjct: 46 DLASGRTLTAWRADERFPMMSTFKVVLCGAVLARVDAGDEQLERKIHYRQQDLVDYSPVS 105 Query: 104 GVKPGRSYTARQLLDGLLLVSGNDAANTLAHMLGGQDVTVAKMNAKAATLGATSTH 159 T +L + +S N AAN L +GG A + A +G T Sbjct: 106 EKHLADGMTVGELCAAAITMSDNSAANLLLATVGG----PAGLTAFLRQIGDNVTR 157
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv2912c	HTHTETR	50	7e-10	TetR bacterial regulatory protein HTH signature.
>HTHTETR#TetR bacterial regulatory protein HTH signature. Length = 215 Score = 50.0 bits (119), Expect = 7e-10 Identities = 23/91 (25%), Positives = 38/91 (41%), Gaps = 4/91 (4%) Query: 1 MARTQQQRREETVARLLQASIDTIIEVGYARASAAVITKRAGVSVGALFRHFETMGDFMA 60 MAR +Q +ET +L ++ + G + S I K AGV+ GA++ HF+ D + Sbjct: 1 MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVTRGAIYWHFKDKSDLFS 60 Query: 61 ATAYEVLRRQLETFTKQVAEIPADRPALPAA 91 + + E A P P + Sbjct: 61 E----IWELSESNIGELELEYQAKFPGDPLS 87
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv2913c	UREASE	34	0.002	Urea amidohydrolase (urease) protein signature.
>UREASE#Urea amidohydrolase (urease) protein signature. Length = 570 Score = 34.3 bits (79), Expect = 0.002 Identities = 24/90 (26%), Positives = 38/90 (42%), Gaps = 19/90 (21%) Query: 17 DVIIRDGLWFDGTGNA--PLTRTLGIRDGVVATVAAGALDETGCPEVVDAAGKWVVPGFI 74 D+ ++DG G A P ++ GV V G EV+ GK V G + Sbjct: 87 DIGLKDGRIA-AIGKAGNP-----DMQPGVTIIVGPGT-------EVIAGEGKIVTAGGM 133 Query: 75 DVHTHYDAEVLLDPGLRESVRHGVTTVLLG 104 D H H+ ++ E++ G+T +L G Sbjct: 134 DSHIHFICPQQIE----EALMSGLTCMLGG 159
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv2914c	YERSSTKINASE	31	0.013	Yersinia serine/threonine protein kinase signature.
>YERSSTKINASE#Yersinia serine/threonine protein kinase signature. Length = 732 Score = 31.2 bits (70), Expect = 0.013 Identities = 37/152 (24%), Positives = 66/152 (43%), Gaps = 25/152 (16%) Query: 69 HPHILEVHDRGEFD----GQLWIAMDYVDG---IDATQHMAD-----RFPAVLPVGEVLA 116 HP++ VH + + MD VDG D + +AD + + G + Sbjct: 190 HPNLANVHGMAVVPYGNRKEEALLMDEVDGWRCSDTLRTLADSWKQGKINSEAYWGTIKF 249 Query: 117 IVTAVAGALDYAHQRGLLHRDVNPANVVLTSQSAGDQRILLADFGIAS----QP-----S 167 I + ++ + G++H D+ P NVV +++G+ ++ D G+ S QP S Sbjct: 250 IAHRLLDVTNHLAKAGVVHNDIKPGNVVF-DRASGEPVVI--DLGLHSRSGEQPKGFTES 306 Query: 168 YPAPELSAG-ADVDGRADQYALALTAIHLFAG 198 + APEL G ++D + + T +H G Sbjct: 307 FKAPELGVGNLGASEKSDVFLVVSTLLHCIEG 338

Rv2930

Rv2934

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
Rv2930	-2	8	-0.271925	Fatty-acid-AMP ligase FadD26 (fatty-acid-AMP
Rv2931	-2	8	-0.073356	Phenolpthiocerol synthesis type-I polyketide
Rv2932	-2	9	-0.217391	Phenolpthiocerol synthesis type-I polyketide
Rv2933	-1	10	-0.495114	Phenolpthiocerol synthesis type-I polyketide
Rv2934	-2	12	-1.299889	Phenolpthiocerol synthesis type-I polyketide

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv2930	FLGMOTORFLIM	29	0.046	Flagellar motor switch protein FliM signature.
>FLGMOTORFLIM#Flagellar motor switch protein FliM signature. Length = 344 Score = 29.1 bits (65), Expect = 0.046 Identities = 18/89 (20%), Positives = 37/89 (41%) Query: 477 DDIEATIQEITGGRAAAIAVPDDITEQLVAIIEFKRRGSTAEEVMLKLRSVKREVTSAIS 536 D+I+ + I+ G A+ + + + +F+R ++E M L + + Sbjct: 8 DEIDQLLTAISSGDASIEDARPISDTRKITLYDFRRPDKFSKEQMRTLSLMHETFARLTT 67 Query: 537 KSHSLRVADLVLVSPGSIPITTSGKIRRS 565 S S ++ +V V S+ T + RS Sbjct: 68 TSLSAQLRSMVHVHVASVDQLTYEEFIRS 96
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv2931	ISCHRISMTASE	31	0.038	Isochorismatase signature.
>ISCHRISMTASE#Isochorismatase signature. Length = 312 Score = 31.1 bits (70), Expect = 0.038 Identities = 15/68 (22%), Positives = 31/68 (45%), Gaps = 1/68 (1%) Query: 1768 LRRIIAAELRVPEKELDTDRPFAELGLNSLMAMAIRREAEQFVGIELSATMLFNHPTVKS 1827 +R+ IA L+ +++ + GL+S+ M + + + G E++ L PT++ Sbjct: 235 IRKQIAELLQETPEDITDQEDLLDRGLDSVRIMTLVEQWRR-EGAEVTFVELAERPTIEE 293 Query: 1828 LASYLAKR 1835 L R Sbjct: 294 WQKLLTTR 301
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv2932	DHBDHDRGNASE	43	4e-06	2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase signature. Length = 261 Score = 43.1 bits (101), Expect = 4e-06 Identities = 30/103 (29%), Positives = 47/103 (45%), Gaps = 1/103 (0%) Query: 1155 LVIGATGNIGPHLIRQLARMGAKTIVAMARKPGALDELTQCLAATGTDLIAVAADATDPA 1214 + GA IG + R LA GA I A+ P L+++ L A A AD D A Sbjct: 12 FITGAAQGIGEAVARTLASQGAH-IAAVDYNPEKLEKVVSSLKAEARHAEAFPADVRDSA 70 Query: 1215 AMQTLFDRFGTELPPLEGIYLAAFAGRPALLSEMTDDDVTTMF 1257 A+ + R E+ P++ + A RP L+ ++D++ F Sbjct: 71 AIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATF 113
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv2933	DHBDHDRGNASE	36	0.001	2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase signature. Length = 261 Score = 36.2 bits (83), Expect = 0.001 Identities = 32/154 (20%), Positives = 61/154 (39%), Gaps = 7/154 (4%) Query: 1805 LIVGGMGGLGFVVARWLAEQGAGLIVLNGRSAPSDEVAAAIAELNASGSRIEVITGDITE 1864 I G G+G VAR LA QGA + +++ ++ L A E D+ + Sbjct: 12 FITGAAQGIGEAVARTLASQGAHIA---AVDYNPEKLEKVVSSLKAEARHAEAFPADVRD 68 Query: 1865 PDTAERLVRAVEDAGFRLAGVVHSAMVLADEIVLNMTDSAARRVFAPKVTGSWRLHVATA 1924 + + +E + +V+ A VL ++ +++D F+ TG + + + Sbjct: 69 SAAIDEITARIEREMGPIDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGVFNASRSVS 128 Query: 1925 ----ARDVDWWLTFSSAAALLGTPGQGAYAAANS 1954 R +T S A + AYA++ + Sbjct: 129 KYMMDRRSGSIVTVGSNPAGVPRTSMAAYASSKA 162
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv2934	DHBDHDRGNASE	35	0.003	2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase sig...
>DHBDHDRGNASE#2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase signature. Length = 261 Score = 34.6 bits (79), Expect = 0.003 Identities = 36/165 (21%), Positives = 63/165 (38%), Gaps = 8/165 (4%) Query: 1437 QGASYVVTGGLGGLGLVVARWLVDRGAGRVVLGGRSDPTDEQCNVLAELQTRAEIVVVRG 1496 +G +TG G+G VAR L +GA + +P + V + Sbjct: 7 EGKIAFITGAAQGIGEAVARTLASQGAH--IAAVDYNPEKLEKVVSSLKAEARHAEAFPA 64 Query: 1497 DVASP-GVAEKLIETARQSGGQLRGVVHAAAVIEDSLVFSMSRDNLERVWAPKATGALRM 1555 DV + E R+ G + +V+ A V+ L+ S+S + E ++ +TG Sbjct: 65 DVRDSAAIDEITARIEREMGP-IDILVNVAGVLRPGLIHSLSDEEWEATFSVNSTGVFNA 123 Query: 1556 HEATADCELDWWLG--FSSAASLLGSP--GQAAYACASAWLDALV 1596 + + +D G + ++ G P AAYA + A Sbjct: 124 SRSVSKYMMDRRSGSIVTVGSNPAGVPRTSMAAYASSKAAAVMFT 168

Rv3343c

Rv3350c

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
Rv3343c	10	17	0.794661	PPE family protein PPE54
Rv3344c	10	19	3.199528	PE-PGRS family protein PE_PGRS49
Rv3345c	10	15	0.945335	PE-PGRS family protein PE_PGRS50
Rv3346c	8	14	-1.238247	Conserved transmembrane protein
Rv3347c	8	14	-1.180532	PPE family protein PPE55
Rv3348	8	14	-0.497492	Probable transposase
Rv3349c	8	14	-0.448566	Probable transposase
Rv3350c	8	15	-0.647955	PPE family protein PPE56

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv3343c	cloacin	32	0.036	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 32.0 bits (72), Expect = 0.036 Identities = 28/79 (35%), Positives = 34/79 (43%), Gaps = 7/79 (8%) Query: 1971 GAGNVGGFNVGGGNIGGNNVGLGNVGWGNFGLG-NSGLTP--GLMGLGNIGFGNAGSYNF 2027 G G+ G + GNI G GLG G + G G +S P G G G G +G N Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNG 65 Query: 2028 GLANMGVGNIGFANTGSGN 2046 G G GN G + GN Sbjct: 66 G----GNGNSGGGSGTGGN 80 Score = 32.0 bits (72), Expect = 0.036 Identities = 29/106 (27%), Positives = 40/106 (37%), Gaps = 4/106 (3%) Query: 193 NVGLFNAGSGNVGSYNVGAGNVGSYNVGGGNIGGNNVGLGNVGWGNFGLGNSGLTPGLMG 252 N G + SGN+ G G G + G G NN G G G G SG G Sbjct: 10 NTGAHST-SGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGN 68 Query: 253 LGNIGFGNAGSYNFG--LANMGVGNIGFANTGSGNFGIGLTGDNLT 296 GN G G+ N A + G + G+G + ++ L+ Sbjct: 69 -GNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALS 113 Score = 31.6 bits (71), Expect = 0.043 Identities = 26/94 (27%), Positives = 37/94 (39%), Gaps = 3/94 (3%) Query: 1842 GNTTGAPSSGFFNSGAGGVSGFGNVGAMVSGGWNQAPSALLGGGSGVFNAGTLHSGVLNF 1901 GN G P+ GA SG+ + GG GGGSG N G + Sbjct: 18 GNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHW--GGGSGHGNGGGNGNSGGGS 75 Query: 1902 GSGMSGLFNTSVLGLGAPALVS-GLGSVGQQLSG 1934 G+G + + + G PAL + G G + +S Sbjct: 76 GTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISA 109
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv3344c	cloacin	34	0.001	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 34.3 bits (78), Expect = 0.001 Identities = 30/99 (30%), Positives = 37/99 (37%), Gaps = 1/99 (1%) Query: 174 GAGGSGGNGGAGGNATGSGGKGGAGGNGGDGS-FGATSGPASIGVTGAPGGNGGKGGAGG 232 G G + G GN G G GG DGS + + + P G GG G G Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNG 65 Query: 233 SNPNGSGGDGGKGGNGGAGGNGGSIGANSGIVGGSGGAG 271 SGG G GGN A + G + G+GG Sbjct: 66 GGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 33.9 bits (77), Expect = 0.001 Identities = 31/80 (38%), Positives = 36/80 (45%), Gaps = 2/80 (2%) Query: 121 TGGSGSGIGGGAGGNGGN--GGAGGTGVVLGGKGGDGGNGDHGGPATNPGSGSRGGAGGS 178 +GG G G GA GN GG G GV G G G + ++ GSG G G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 179 GGNGGAGGNATGSGGKGGAG 198 GNGG GN+ G G GG Sbjct: 62 HGNGGGNGNSGGGSGTGGNL 81 Score = 33.9 bits (77), Expect = 0.001 Identities = 36/121 (29%), Positives = 41/121 (33%), Gaps = 13/121 (10%) Query: 13 GAGGNGGAGSAGNGGAGGAGGNGGAGGNGGGGDAGNAGSGGNGGKGGDGVGPGSTGGAGG 72 G G G A + GG G G GG D S N GG G G GG+G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 73 KGGAGANGGSSNGNARGGNAGNGGHGGAGGSGDTGGAGGAGGQGGFGGTGGSGSGIGGGA 132 G GGN +GG G GG+ A A G G G + A Sbjct: 63 GNG-------------GGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISA 109 Query: 133 G 133 G Sbjct: 110 G 110 Score = 33.9 bits (77), Expect = 0.001 Identities = 27/79 (34%), Positives = 35/79 (44%), Gaps = 1/79 (1%) Query: 69 GAGGKGGAGANGGSSNGNARGGNAGNGGHGGAG-GSGDTGGAGGAGGQGGFGGTGGSGSG 127 G G GA + G+ NG G G G G+G S + GG+G +GG G G+G Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNG 65 Query: 128 IGGGAGGNGGNGGAGGTGV 146 G G G G G + V Sbjct: 66 GGNGNSGGGSGTGGNLSAV 84 Score = 33.1 bits (75), Expect = 0.003 Identities = 28/90 (31%), Positives = 31/90 (34%), Gaps = 4/90 (4%) Query: 240 GDGGKGGNGGAGGNGGSIGANSGIVGGSGGAGGAGGAGG----NGSLSSGEGGKGGDGGH 295 G G+G N GA G+I +G GGA G G S GG GH Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 296 GGDGVGGNSSVTQGGSGGGGGAGGAGGSGF 325 G G GNS G G GF Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFGF 92 Score = 32.8 bits (74), Expect = 0.003 Identities = 27/84 (32%), Positives = 33/84 (39%) Query: 193 GKGGAGGNGGDGSFGATSGPASIGVTGAPGGNGGKGGAGGSNPNGSGGDGGKGGNGGAGG 252 G G G N G S G+ G + G G + +NP G G G GG+G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 253 NGGSIGANSGIVGGSGGAGGAGGA 276 G NSG G+GG A A Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAA 86 Score = 31.2 bits (70), Expect = 0.009 Identities = 22/69 (31%), Positives = 27/69 (39%) Query: 4 SPAAHGGSGGAGGNGGAGSAGNGGAGGAGGNGGAGGNGGGGDAGNAGSGGNGGKGGDGVG 63 P G GGA G S N GG+G GG G G+ G G+ G G G + Sbjct: 23 GPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNGNSGGGSGTGGNLS 82 Query: 64 PGSTGGAGG 72 + A G Sbjct: 83 AVAAPVAFG 91 Score = 30.8 bits (69), Expect = 0.012 Identities = 30/84 (35%), Positives = 36/84 (42%), Gaps = 5/84 (5%) Query: 223 GNGGKGGAGGSNPNGSGGDGGKGGNGGAGGNGGSIGANSGIVGGSGGAGGAGGAGGNGSL 282 G G GA ++ N +GG G G GGA G N+ GGSG GG G Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG---- 61 Query: 283 SSGEGGKGGDGGHGGDGVGGNSSV 306 G GG G+ G G G S+V Sbjct: 62 -HGNGGGNGNSGGGSGTGGNLSAV 84 Score = 30.8 bits (69), Expect = 0.012 Identities = 31/80 (38%), Positives = 34/80 (42%), Gaps = 7/80 (8%) Query: 83 SNGNARGGNAGNGGHGGAGGSGDTGGAGGAGGQGGFGGT-------GGSGSGIGGGAGGN 135 S G+ RG N G G G TG G G G G + GGSGSGI G G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 136 GGNGGAGGTGVVLGGKGGDG 155 GNGG G G GG+ Sbjct: 62 HGNGGGNGNSGGGSGTGGNL 81 Score = 30.8 bits (69), Expect = 0.015 Identities = 30/83 (36%), Positives = 39/83 (46%), Gaps = 1/83 (1%) Query: 290 GGDG-GHGGDGVGGNSSVTQGGSGGGGGAGGAGGSGFFGGKGGFGGDGGQGGPNGGGTVG 348 GGDG GH + ++ G +G G G G + GSG+ +GG G G GGG+ Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 349 TVAGGGGNGGVGGRGGDGVFAGA 371 GG GN G G G + A A Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVA 85 Score = 30.5 bits (68), Expect = 0.019 Identities = 32/84 (38%), Positives = 33/84 (39%), Gaps = 5/84 (5%) Query: 333 GGDGGQGGPNGGGTVGTVAGGGGNGGVGGRGGDGVFAGAGGQGGLGGQGGNGGGSTGGNG 392 GGDG T G + GG GVGG DG G G G GS G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDG-----SGWSSENNPWGGGSGSGIHWG 57 Query: 393 GLGGAGGGGGNAPDGGFGGNGGKG 416 G G G GGGN GG G GG Sbjct: 58 GGSGHGNGGGNGNSGGGSGTGGNL 81 Score = 30.1 bits (67), Expect = 0.021 Identities = 25/86 (29%), Positives = 31/86 (36%) Query: 369 AGAGGQGGLGGQGGNGGGSTGGNGGLGGAGGGGGNAPDGGFGGNGGKGGQGGIGGGTQSA 428 +G G+G G G GG GLG GG + G G GI G S Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 429 TGLGGDGGDGGDGGNGGNSGAKAGGA 454 G GG G+ G G G + + Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAAP 87 Score = 30.1 bits (67), Expect = 0.023 Identities = 33/109 (30%), Positives = 40/109 (36%), Gaps = 9/109 (8%) Query: 108 GAGGAGGQGGFGGTGGSGSGIGGGAGGNGGNGGAGGTGVVLGGKGGDGGNGDHGGPATNP 167 G G G G T G+ +G G G GG G GG G+G H G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGG---- 58 Query: 168 GSGSRGGAGGSGGNGGAGGNATGSGGKGGAGGNGGDGSFGATSGPASIG 216 G G+GG G G +G+GG A F A S P + G Sbjct: 59 -----GSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG 102
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv3345c	cloacin	40	8e-05	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 40.1 bits (93), Expect = 8e-05 Identities = 31/104 (29%), Positives = 37/104 (35%), Gaps = 2/104 (1%) Query: 1174 LGGNGGAGGNGGVSTTGGD--GGAGGKGGNGGDGGNVGLGGDAGSGGAGGNGGIGTDAGG 1231 + G G G N G +T G+ GG G G GG G + G G GI G Sbjct: 1 MSGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGS 60 Query: 1232 AGGAGGAGGNGGSSKSTTTGNAGSGGAGGNGGTGLNGAGGAGGA 1275 G GG GN G T + G L+ G G A Sbjct: 61 GHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 39.7 bits (92), Expect = 8e-05 Identities = 41/121 (33%), Positives = 50/121 (41%), Gaps = 5/121 (4%) Query: 1311 AGGKGGNGSSGAASGSGVVNVTAGHGGNGGNGGNGGNGSAGAGGQGGAGGSAGNGGHGGG 1370 +GG G ++GA S SG +N G GG +G S+ GG GS + G G G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 1371 ATGGDGGNGGNGGNSGNSTGVAGLAGGAAGAGGNGGGTSSAAGHGGSGGSGGSGTTGGAG 1430 GNGG GNSG +G G A G S G GG S +G A Sbjct: 62 -----HGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAAI 116 Query: 1431 A 1431 A Sbjct: 117 A 117 Score = 38.5 bits (89), Expect = 2e-04 Identities = 36/100 (36%), Positives = 46/100 (46%), Gaps = 2/100 (2%) Query: 962 GNGGNGGNGGKGGTAGNGSGAAGGNGGNGG-SGLNGGDAGNGGNGGGALNQAGFFGTGGK 1020 G G G N G T+GN +G G G GG S +G + N GGG+ + + G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 1021 GGNGGNGGAGMINGGLGGFGGAGGGGAVDVAA-TTGGAGG 1059 G GGNG +G +G G A A +T GAGG Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG 102 Score = 37.8 bits (87), Expect = 4e-04 Identities = 38/104 (36%), Positives = 43/104 (41%), Gaps = 3/104 (2%) Query: 562 SGGNGGNGGAGATPTVAGENGGAGGNGGHGGSVGNGGAGGAGGNGVAGTGLALNGGNGGN 621 SGG+G GA T NGG G G GG + G+G + N G G GG Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGG--ASDGSGWSSENNPWGGGSGSGIHWGGG 59 Query: 622 GGIGGNGGSAAGTGGDGGKGGNGGAGANGQDFSASANGANGGQG 665 G GNGG +GG G GGN A A F A G G Sbjct: 60 SG-HGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG 102 Score = 37.0 bits (85), Expect = 6e-04 Identities = 26/85 (30%), Positives = 34/85 (40%) Query: 599 AGGAGGNGVAGTGLALNGGNGGNGGIGGNGGSAAGTGGDGGKGGNGGAGANGQDFSASAN 658 +GG G G NGG G+G GG++ G+G GG +G + + Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 659 GANGGQGGNGGNGGIGGKGGDAFAT 683 NGG GN G G G A A Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAA 86 Score = 36.6 bits (84), Expect = 9e-04 Identities = 34/109 (31%), Positives = 44/109 (40%), Gaps = 3/109 (2%) Query: 1167 GPEGNGGLGGNGGAGGNGGVSTTGGDGGAGGKGGNGGDGGNVGLGGDAGSG---GAGGNG 1223 G +G G G GN TG G G G+G N GG +GSG G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 1224 GIGTDAGGAGGAGGAGGNGGSSKSTTTGNAGSGGAGGNGGTGLNGAGGA 1272 G G G +GG G GGN + + + G GG ++ + GA Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGA 111 Score = 36.2 bits (83), Expect = 0.001 Identities = 35/116 (30%), Positives = 40/116 (34%), Gaps = 8/116 (6%) Query: 1337 GNGGNGGNGGNGSAGAGGQGGAGGSAGNGGHGGGATGGDGGNGGNGGNSGNSTGVAGLAG 1396 G G G N G S GG G GG G +G N GG SG+ G +G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDG-SGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 1397 GAAGAGGNGGGTSSAAGHGGSGGSGGSGTTGGAGAAGGNGGAGAGGGSLSTGQSGG 1452 G G G GGSG G A G + G G L+ S G Sbjct: 62 HGNGGGNGNSG-------GGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAG 110 Score = 35.8 bits (82), Expect = 0.001 Identities = 29/80 (36%), Positives = 34/80 (42%), Gaps = 3/80 (3%) Query: 1291 GGDGGNGGNGGHGGDGTTGGAGGKGGNGSSGAASGSG--VVNVTAGHGGNGGNGGNGGNG 1348 GGDG G H G G G GA+ GSG N G G G GG+G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLG-VGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 1349 SAGAGGQGGAGGSAGNGGHG 1368 GG G +GG +G GG+ Sbjct: 62 HGNGGGNGNSGGGSGTGGNL 81 Score = 35.8 bits (82), Expect = 0.002 Identities = 24/76 (31%), Positives = 32/76 (42%) Query: 747 GNGGDGGKGGSGGNVGNGGNGGAGGNGAAGQAGTPGPTSGDSGTSGTDGGAGGNGGAGGA 806 G G + G + GN+ G G G GA+ +G + G SG+ GG G G Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNG 65 Query: 807 GGTLAGHGGNGGKGGN 822 GG GG+G G Sbjct: 66 GGNGNSGGGSGTGGNL 81 Score = 35.1 bits (80), Expect = 0.003 Identities = 28/97 (28%), Positives = 33/97 (34%), Gaps = 1/97 (1%) Query: 796 GAGGNGGAGGAGGTLAGHGGNGGKGGNGGQG-GIGGAGERGADGAGPNANGANGENGGSG 854 G G N GA G + G G GG G G G+G + G G+G Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNG 65 Query: 855 GNGGDGGAGGNGGAGGKAQAAGYTDGATGTGGDGGNG 891 G G+ G G G A AA G G G Sbjct: 66 GGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG 102 Score = 35.1 bits (80), Expect = 0.003 Identities = 27/83 (32%), Positives = 34/83 (40%) Query: 1350 AGAGGQGGAGGSAGNGGHGGGATGGDGGNGGNGGNSGNSTGVAGLAGGAAGAGGNGGGTS 1409 +G G+G G+ G+ G G G GG SG S+ GG+ GGG+ Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 1410 SAAGHGGSGGSGGSGTTGGAGAA 1432 G G GGSGT G A Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAV 84 Score = 34.7 bits (79), Expect = 0.003 Identities = 29/104 (27%), Positives = 31/104 (29%) Query: 1136 LGGNGGLGGNGGVSETGFGGAGGNGGYGGPGGPEGNGGLGGNGGAGGNGGVSTTGGDGGA 1195 + G G G N G T GG G G GG G G G S GG+ Sbjct: 1 MSGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGS 60 Query: 1196 GGKGGNGGDGGNVGLGGDAGSGGAGGNGGIGTDAGGAGGAGGAG 1239 G G G G G G A GAGG Sbjct: 61 GHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 34.7 bits (79), Expect = 0.003 Identities = 36/102 (35%), Positives = 40/102 (39%), Gaps = 3/102 (2%) Query: 1056 GAGGNGGAGGFASTGLGGPGGAGGPGGAGDFASGVGGVGGAGGDGGAGGVGGFGGQGGIG 1115 G G N GA + GGP G G GGA D SG G G G+ GG G Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGGASD-GSGWSSENNPWGGGSGSGIHWGGGSGHGN 64 Query: 1116 GEGRTGGNGGSGGDGGGGISLGGNGGLGGNGGVSETGFGGAG 1157 G G GN G G GG +S G +S G GG Sbjct: 65 GGG--NGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 34.3 bits (78), Expect = 0.004 Identities = 31/110 (28%), Positives = 37/110 (33%), Gaps = 10/110 (9%) Query: 379 GGIGATANSPLQAGGAGGNGGHGGLVGNGGTGGAGGAGHAGSTGATGTALQPTGGNGTNG 438 GG G N+ + NGG GL GG G + G+ G G+ Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 439 GAGGHGGNGGNGGAQHGDGGVGGKGGAGGSGGAGGNGFDAATLGSPGADG 488 G GG GN G G G S A F L +PGA G Sbjct: 63 GNGGGNGNSGGGSG----------TGGNLSAVAAPVAFGFPALSTPGAGG 102 Score = 34.3 bits (78), Expect = 0.004 Identities = 28/101 (27%), Positives = 34/101 (33%), Gaps = 1/101 (0%) Query: 826 GGIGGAGERGADGAGPNANGANGENGGSGGNGGDGGAGGNGGAGGKAQAAGYTDGATGTG 885 GG G GA N NG G GG G G +G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 886 GDGGNGGDGGKAGDGGAGENGLNSGAMLPGGGTVGNPGTGG 926 G+GG G+ G G G G + + G + PG GG Sbjct: 63 GNGGGNGNSG-GGSGTGGNLSAVAAPVAFGFPALSTPGAGG 102 Score = 34.3 bits (78), Expect = 0.004 Identities = 37/126 (29%), Positives = 48/126 (38%), Gaps = 10/126 (7%) Query: 1284 VSFGNAVGGDGGNGGNGGHGGDGTTGGAGGKGGNGSS---------GAASGSGVVNVTAG 1334 +S G+ G + G G+ G TG G G + S G SGSG+ Sbjct: 1 MSGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGS 60 Query: 1335 HGGNGGNGGNGGNGSAGAGGQGGAGGSAGNGGHGGGATGGDGGNGGNGGNSGNSTGVAGL 1394 GNGG GN G GS G GG A + G +T G GG + S +A + Sbjct: 61 GHGNGGGNGNSGGGS-GTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAAIADI 119 Query: 1395 AGGAAG 1400 G Sbjct: 120 MAALKG 125 Score = 33.9 bits (77), Expect = 0.005 Identities = 25/78 (32%), Positives = 31/78 (39%) Query: 1211 GGDAGSGGAGGNGGIGTDAGGAGGAGGAGGNGGSSKSTTTGNAGSGGAGGNGGTGLNGAG 1270 GGD G + G GG G G GG S ++ N GG+G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 1271 GAGGAGGNAGVAGVSFGN 1288 G GG GN+G + GN Sbjct: 63 GNGGGNGNSGGGSGTGGN 80 Score = 33.9 bits (77), Expect = 0.006 Identities = 24/86 (27%), Positives = 30/86 (34%) Query: 1347 NGSAGAGGQGGAGGSAGNGGHGGGATGGDGGNGGNGGNSGNSTGVAGLAGGAAGAGGNGG 1406 +G G G GA ++GN G G GG G S + G +G GG G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 1407 GTSSAAGHGGSGGSGGSGTTGGAGAA 1432 + GGSG G A Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAAP 87 Score = 33.5 bits (76), Expect = 0.008 Identities = 25/71 (35%), Positives = 34/71 (47%) Query: 876 GYTDGATGTGGDGGNGGDGGKAGDGGAGENGLNSGAMLPGGGTVGNPGTGGNGGNGGNAG 935 G+ GA T G+ G G G G + +G +S GGG+ GG G+G G Sbjct: 8 GHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGG 67 Query: 936 VGGTGGKAGTG 946 G +GG +GTG Sbjct: 68 NGNSGGGSGTG 78 Score = 33.1 bits (75), Expect = 0.008 Identities = 41/126 (32%), Positives = 44/126 (34%), Gaps = 9/126 (7%) Query: 1096 AGGDGGAGGVGGFGGQGGIGGEGRTGGNGGSGGDGGGGISLGGNGGLGGNGGVSETGFGG 1155 +GGDG G G I G G TG G G G G S N GG+G G G Sbjct: 2 SGGDGRGHNTGAHSTSGNING-GPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGS 60 Query: 1156 AGGNGGYGGPGGPEGNGGLGGNGGAGGNGGVSTTGGDGGAGGKGGNGGDGGNVGLGGDAG 1215 GNGG GNG GG G GGN G G G V + A Sbjct: 61 GHGNGG--------GNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGAL 112 Query: 1216 SGGAGG 1221 S Sbjct: 113 SAAIAD 118 Score = 33.1 bits (75), Expect = 0.010 Identities = 32/102 (31%), Positives = 38/102 (37%) Query: 917 GTVGNPGTGGNGGNGGNAGVGGTGGKAGTGSLTGLDGTDGITPNGGNGGNGGNGGKGGTA 976 G G G GN G TG G G+ G + P GG G+G + G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 977 GNGSGAAGGNGGNGGSGLNGGDAGNGGNGGGALNQAGFFGTG 1018 GNG G GG+G G A G AL+ G G Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 33.1 bits (75), Expect = 0.010 Identities = 35/102 (34%), Positives = 44/102 (43%), Gaps = 1/102 (0%) Query: 175 GSGGAGAAGGVGGSGGWLNGNGGAGGAGGTGANGGAGGNAWLFGAGGSGGAGTNGGVGGS 234 G G G G + G +NG G GG GA+ G+G ++ GG G+G + G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGG-GASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 235 GGFVYGNGGAGGIGGIGGIGGNGGDAGLFGNGGAGGAGAAGL 276 G GNG +GG G GG FG GA GL Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGL 103 Score = 32.0 bits (72), Expect = 0.020 Identities = 39/114 (34%), Positives = 47/114 (41%), Gaps = 4/114 (3%) Query: 285 GDGSDGGNGGTGGN--GGRGGLLVGNGGAGGAG-GVGGDGGKGGAGDPSFAVNNGAGGNG 341 G G + G T GN GG GL VG G + G+G + GG+G GNG Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNG 65 Query: 342 GHGGNPGVGGAGGAGGLLAGAHGAAGATPTSGGNGGDGGIGATANSPLQAGGAG 395 G GN G GG+G G L A A A P G G + + L A A Sbjct: 66 GGNGNSG-GGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAAIAD 118 Score = 32.0 bits (72), Expect = 0.021 Identities = 27/86 (31%), Positives = 32/86 (37%), Gaps = 7/86 (8%) Query: 901 GAGENGLNSGAMLPGGGTVGNPGTGGNGGNGGNAGVGGTGGKAGTGSLTGLDGTDGITPN 960 G G N+GA G G P G GG G + G + G G + Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGG-------GASDGSGWSSENNPWGGGSGSGIH 55 Query: 961 GGNGGNGGNGGKGGTAGNGSGAAGGN 986 G G GNGG G +G GSG G Sbjct: 56 WGGGSGHGNGGGNGNSGGGSGTGGNL 81 Score = 31.6 bits (71), Expect = 0.025 Identities = 39/121 (32%), Positives = 47/121 (38%), Gaps = 8/121 (6%) Query: 1115 GGEGRTGGNGGSGGDGGGGISLGGNGGLGGNGGVSETGFGGAGGNGGYGGPGGPEGNGGL 1174 GG+GR G+ G I+ GG GLG GG S+ + N GG G GG Sbjct: 3 GGDGR--GHNTGAHSTSGNIN-GGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGG 59 Query: 1175 GGNGGAGGNGGVSTTGGDGGAGGKGGNGGDGGNVGLGGDAGSGGAGGNGGIGTDAGGAGG 1234 G+G GGNG GG+G G V G A S G + AG Sbjct: 60 SGHGNGGGNGN-----SGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSA 114 Query: 1235 A 1235 A Sbjct: 115 A 115 Score = 31.6 bits (71), Expect = 0.028 Identities = 29/102 (28%), Positives = 35/102 (34%), Gaps = 2/102 (1%) Query: 1197 GKGGNGGDGGNVGLGGDAGSGGAGGNGGIGTDAGGAGGAGGAGGNGGSSKSTTTGNAGSG 1256 G G G + G G+ G G G G G + GGS G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 1257 GAGGNGGTGLNGAGGAGGAGGNAGVAGVSFGNAVGGDGGNGG 1298 G GG G G+G G +A A V+FG G GG Sbjct: 63 GNGGGNGNSGGGSGTGG--NLSAVAAPVAFGFPALSTPGAGG 102 Score = 31.6 bits (71), Expect = 0.032 Identities = 21/71 (29%), Positives = 27/71 (38%) Query: 442 GHGGNGGNGGAQHGDGGVGGKGGAGGSGGAGGNGFDAATLGSPGADGGMGGNGGKGGDGG 501 G G G N GA G + G G GG +G ++ +P G G GG G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 502 KAGDGGAGAAG 512 G G + G Sbjct: 63 GNGGGNGNSGG 73 Score = 31.2 bits (70), Expect = 0.033 Identities = 25/78 (32%), Positives = 32/78 (41%), Gaps = 1/78 (1%) Query: 1016 GTGGKGGNGG-NGGAGMINGGLGGFGGAGGGGAVDVAATTGGAGGNGGAGGFASTGLGGP 1074 G G+G N G + +G INGG G G GG ++ G G G G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 1075 GGAGGPGGAGDFASGVGG 1092 G GG G +G + G Sbjct: 63 GNGGGNGNSGGGSGTGGN 80 Score = 31.2 bits (70), Expect = 0.034 Identities = 26/79 (32%), Positives = 32/79 (40%) Query: 221 GSGGAGTNGGVGGSGGFVYGNGGAGGIGGIGGIGGNGGDAGLFGNGGAGGAGAAGLPGAA 280 G G G N G + G + G G+GG G GG+G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 281 GLNGGDGSDGGNGGTGGNG 299 G GG+G+ GG GTGGN Sbjct: 63 GNGGGNGNSGGGSGTGGNL 81 Score = 31.2 bits (70), Expect = 0.041 Identities = 32/104 (30%), Positives = 35/104 (33%) Query: 250 IGGIGGNGGDAGLFGNGGAGGAGAAGLPGAAGLNGGDGSDGGNGGTGGNGGRGGLLVGNG 309 + G G G + G G G GL G + G G N GG G G G Sbjct: 1 MSGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGS 60 Query: 310 GAGGAGGVGGDGGKGGAGDPSFAVNNGAGGNGGHGGNPGVGGAG 353 G G GG G GG G G AV PG GG Sbjct: 61 GHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 30.8 bits (69), Expect = 0.043 Identities = 35/111 (31%), Positives = 40/111 (36%), Gaps = 2/111 (1%) Query: 538 GGAGGVSANPALNGSAGANGTAPTSGGNGGNGGAGATPTVAGENGGAGGNGGHGGSVGNG 597 GG G A + S NG G GG + GG G+G H G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWG--GGS 60 Query: 598 GAGGAGGNGVAGTGLALNGGNGGNGGIGGNGGSAAGTGGDGGKGGNGGAGA 648 G G GGNG +G G G G A T G GG + AGA Sbjct: 61 GHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGA 111
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv3347c	cloacin	32	0.034	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 32.4 bits (73), Expect = 0.034 Identities = 24/91 (26%), Positives = 33/91 (36%), Gaps = 8/91 (8%) Query: 363 GFNTGVANVGSYNTGSFNAGNTNTGGFNPGNVNTGWLNTGNTNTGIANSGNVNTGAFISG 422 G NTG + G+ N G T G + +GW + N G + SG G G Sbjct: 8 GHNTGAHSTS----GNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHG 63 Query: 423 NFSNGVLWRGDYEGLWGLSGGSTIPAIPIGL 453 N G+ G G G + A P+ Sbjct: 64 NGGGN----GNSGGGSGTGGNLSAVAAPVAF 90
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv3349c	INTIMIN	31	0.005	Intimin signature.
>INTIMIN#Intimin signature. Length = 939 Score = 30.8 bits (69), Expect = 0.005 Identities = 19/67 (28%), Positives = 33/67 (49%), Gaps = 4/67 (5%) Query: 4 DPAAAYASAIRTPGLLPNAKLVVDHFHVTTLANDALTAVRRRVTWAFHDRRGRKIDPQWA 63 +P AA TP +P + +D+ H T ND L +++ + F ++I+PQ+ Sbjct: 369 NPGAATVGVNYTP--IPLVTMGIDYRHGTGNENDLLYSMQ--FRYQFDKPWSQQIEPQYV 424 Query: 64 NRRRLLT 70 N R L+ Sbjct: 425 NELRTLS 431
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv3350c	PYOCINKILLER	37	0.002	Pyocin S killer protein signature.
>PYOCINKILLER#Pyocin S killer protein signature. Length = 617 Score = 36.7 bits (84), Expect = 0.002 Identities = 46/199 (23%), Positives = 68/199 (34%), Gaps = 17/199 (8%) Query: 3442 ISVPSIHLGLDPAVHVGSITVNPITVRTPPVLVSYSQGAVTSTSGPTSEIWVKPSFFPGI 3501 + + + LGL P+V++ ++ TV P L + ++G T+ S +++ P P Sbjct: 328 LGMDAAKLGLPPSVNLNAVAKASGTVDLPMRLTNEARGNTTTLSVVSTDGVSVPKAVPVR 387 Query: 3502 RIAPSSGGGATSTQGAYFVGPISIPSGTVTFPGFTIPLDPIDIGLPVSLT--IP-GFTI- 3557 A +T G Y V S + P P P S T +P + Sbjct: 388 MAAY------NATTGLYEVTVPSTTAEAPPLILTWTPASPPGNQNPSSTTPVVPKPVPVY 441 Query: 3558 PGGTLIPTLPLGLALSNGIPPVDIPAIVLDRILLDLHADTTIGPINVPIAGFGGAPGFGN 3617 G TL P I + I AD+ I PI V PG Sbjct: 442 EGATLTPVKATPETYPGVITLPEDLIIGFP-------ADSGIKPIYVMFRDPRDVPGAAT 494 Query: 3618 STTLPSSGFFNTGAGGGSG 3636 P SG + A G G Sbjct: 495 GKGQPVSGNWLGAASQGEG 513

Rv3420c

Rv3427c

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
Rv3420c	1	17	-0.379903	Ribosomal-protein-alanine acetyltransferase RimI
Rv3421c	0	20	-2.258486	Conserved hypothetical protein
Rv3422c	-1	18	-3.173656	Conserved hypothetical protein
Rv3423c	-1	18	-2.848693	Alanine racemase Alr
Rv3424c	1	23	-4.644413	Hypothetical protein
Rv3425	0	19	-3.478445	PPE family protein PPE57
Rv3426	-1	18	-2.630668	PPE family protein PPE58
Rv3427c	0	17	-1.291636	Possible transposase

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv3420c	SACTRNSFRASE	43	5e-08	Streptothricin acetyltransferase signature.
>SACTRNSFRASE#Streptothricin acetyltransferase signature. Length = 173 Score = 43.4 bits (102), Expect = 5e-08 Identities = 15/66 (22%), Positives = 28/66 (42%), Gaps = 3/66 (4%) Query: 79 VHTIGVDPAYQGRGIGRRLLRELLDFARG---GVVYLEVRTDNDAALALYRSVGFQRVGL 135 + I V Y+ +G+G LL + +++A+ + LE + N +A Y F + Sbjct: 92 IEDIAVAKDYRKKGVGTALLHKAIEWAKENHFCGLMLETQDINISACHFYAKHHFIIGAV 151 Query: 136 RRRYYR 141 Y Sbjct: 152 DTMLYS 157
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv3422c	ABC2TRNSPORT	31	0.002	ABC-2 type transport system membrane protein signat...
>ABC2TRNSPORT#ABC-2 type transport system membrane protein signature. Length = 262 Score = 30.7 bits (69), Expect = 0.002 Identities = 15/47 (31%), Positives = 21/47 (44%), Gaps = 1/47 (2%) Query: 22 ATLPRVEDTLTLGSRLGEQLCAGDVVVLSGPLGAGKTVLAKGIAMAM 68 A R+E T + L QL GD+V+ A K LA G + + Sbjct: 89 AAFGRMEGQRTWEAMLYTQLRLGDIVLGEMAWAATKAALA-GAGIGV 134
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv3423c	ALARACEMASE	425	e-151	Alanine racemase signature.
>ALARACEMASE#Alanine racemase signature. Length = 356 Score = 425 bits (1094), Expect = e-151 Identities = 119/373 (31%), Positives = 179/373 (47%), Gaps = 28/373 (7%) Query: 36 AEAMVDLGAIEHNVRVLREHAGHAQLMAVVKADGYGHGATRVAQTALGAGAAELGVATVD 95 +A +DL A++ N+ ++R+ A HA++ +VVKA+ YGHG R+ + ++ Sbjct: 5 IQASLDLQALKQNLSIVRQAATHARVWSVVKANAYGHGIERIWSAIGATDG--FALLNLE 62 Query: 96 EALALRADGITAPVL---AWLHPPGIDFGPALLADVQVAVSSLRQLDELLHAVRRTGRTA 152 EA+ LR G P+L + H ++ + V S QL L +A R Sbjct: 63 EAITLRERGWKGPILMLEGFFHAQDLEIYDQ--HRLTTCVHSNWQLKALQNA--RLKAPL 118 Query: 153 TVTVKVDTGLNRNGVGPAQFPAMLTALRQAMAEDAVRLRGLMSHMVYADKPDDSINDVQA 212 + +KV++G+NR G P + +LT +Q A V LMSH A+ PD + Sbjct: 119 DIYLKVNSGMNRLGFQPDR---VLTVWQQLRAMANVGEMTLMSHFAEAEHPDGISGAM-- 173 Query: 213 QRFTAFLAQAREQGVRFEVAHLSNSSATMARPDLTFDLVRPGIAVYGLSPVPALGDM--- 269 A + QA E G+ LSNS+AT+ P+ FD VRPGI +YG SP D+ Sbjct: 174 ----ARIEQAAE-GLECRR-SLSNSAATLWHPEAHFDWVRPGIILYGASPSGQWRDIANT 227 Query: 270 GLVPAMTVKCAVALVKSIRAGEGVSYGHTWIAPRDTNLALLPIGYADGVFRSLGGRLEVL 329 GL P MT+ + V++++AGE V YG + A + + ++ GYADG R VL Sbjct: 228 GLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGYPRHAPTGTPVL 287 Query: 330 INGRRCPGVGRICMDQFMVDLGPGPLDVAEGDEAILFGPGIRGEPTAQDWADLVGTIHYE 389 ++G R VG + MD VDL P P G L+G E D A GT+ YE Sbjct: 288 VDGVRTMTVGTVSMDMLAVDLTPCP-QAGIGTPVELWGK----EIKIDDVAAAAGTVGYE 342 Query: 390 VVTSPRGRITRTY 402 ++ + R+ Sbjct: 343 LMCALALRVPVVT 355
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv3425	SECBCHAPRONE	28	0.023	Bacterial protein-transport SecB chaperone protein ...
>SECBCHAPRONE#Bacterial protein-transport SecB chaperone protein signature. Length = 170 Score = 27.6 bits (61), Expect = 0.023 Identities = 12/50 (24%), Positives = 26/50 (52%), Gaps = 1/50 (2%) Query: 5 IPAEYISNIIYEGPGADSLFFASGQLRELAYSVETTAESLEDELDELDEN 54 I Y+ ++ +E P +F + +L++ + T A+ + D+L E+ N Sbjct: 22 IQRIYVKDVSFEAPNLPHIFQQDWE-PKLSFDLSTEAKQVGDDLYEVCLN 70
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv3427c	HTHFIS	28	0.045	FIS bacterial regulatory protein HTH signature.
>HTHFIS#FIS bacterial regulatory protein HTH signature. Length = 484 Score = 27.9 bits (62), Expect = 0.045 Identities = 20/84 (23%), Positives = 29/84 (34%), Gaps = 4/84 (4%) Query: 48 DEIARRESAALTRRLRRAKFEAQATFEDFDFTANPKLPGAMLRDLAALRWLDAGE-SVIL 106 E+ AL RR + + AM L L + ++++ Sbjct: 109 TELIGIIGRALAEPKRRPSKLEDDSQDGMPLVGRSA---AMQEIYRVLARLMQTDLTLMI 165 Query: 107 HGPVGVGKTHVAQALVHAVARRGG 130 G G GK VA+AL RR G Sbjct: 166 TGESGTGKELVARALHDYGKRRNG 189

Rv3507

Rv3517

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
Rv3507	17	32	11.772814	PE-PGRS family protein PE_PGRS53
Rv3508	14	29	10.986745	PE-PGRS family protein PE_PGRS54
Rv3509c	14	30	10.655543	Probable acetohydroxyacid synthase IlvX
Rv3510c	12	31	10.087774	Conserved protein
Rv3511	12	32	10.290023	PE-PGRS family protein PE_PGRS55
Rv3512	9	31	9.493548	PE-PGRS family protein PE_PGRS56
Rv3513c	4	25	6.507313	Probable fatty-acid-CoA ligase FadD18 (fragment)
Rv3514	4	25	6.485584	PE-PGRS family protein PE_PGRS57
Rv3515c	-1	13	-0.225502	Fatty-acid-CoA ligase FadD19 (fatty-acid-CoA
Rv3516	-1	13	0.241866	Possible enoyl-CoA hydratase EchA19 (enoyl
Rv3517	0	12	0.058828	Conserved hypothetical protein

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv3507	cloacin	39	1e-04	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 38.9 bits (90), Expect = 1e-04 Identities = 35/103 (33%), Positives = 43/103 (41%) Query: 1172 NGGNGGNGNDGAVNAGTGGSGGNGGNAGGGGANGGDGGAGGAGGAGGRGGKGIDGGFGGD 1231 +GG+G N GA + +GG G GGGA+ G G + GG G GI G G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 1232 GGNGGSNNGTGAGGNGGNGGTGGVGSVGAAGGDGGNGGTGGFA 1274 GNGG N +G G G + V G GG A Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 37.8 bits (87), Expect = 3e-04 Identities = 30/79 (37%), Positives = 35/79 (44%) Query: 1154 GKGGDGGNIAAGATGTAGNGGNGGNGNDGAVNAGTGGSGGNGGNAGGGGANGGDGGAGGA 1213 G G G N A +T NGG G G G + G+G S N GG G+ GG G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 1214 GGAGGRGGKGIDGGFGGDG 1232 G GG G G G GG+ Sbjct: 63 GNGGGNGNSGGGSGTGGNL 81 Score = 37.0 bits (85), Expect = 5e-04 Identities = 34/99 (34%), Positives = 37/99 (37%), Gaps = 1/99 (1%) Query: 837 GNAGDGGNGGNAGAGGNGGSGDFGGNTTSGAS-GSGGNGGNAGTAGSGGAGGTGGTGLSG 895 G G G N G GN G G GAS GSG + N G G+G G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 896 GNGGNGGNGGNGGDGGNGAHGTVGAQFVPATSLPTPNGG 934 GNGG GN G G G +L TP G Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAG 101 Score = 37.0 bits (85), Expect = 5e-04 Identities = 35/109 (32%), Positives = 47/109 (43%), Gaps = 1/109 (0%) Query: 518 TGGNGGNGTGGVNGADNTLNPDTPGGAGEPGGAGGAGGAGGAAGGPGGTGGTGGNGGNGG 577 +GG+G G + +N G G + G+G + GG+G GG G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 578 NGGNGGNGGNGGNGGNAGNNSTNA-PVGGEGGAGGDGGAGGAGGAANGG 625 +G GGNG +GG G GN S A PV A GAGG + + G Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAG 110 Score = 36.2 bits (83), Expect = 0.001 Identities = 28/76 (36%), Positives = 33/76 (43%) Query: 1148 GNGSAGGKGGDGGNIAAGATGTAGNGGNGGNGNDGAVNAGTGGSGGNGGNAGGGGANGGD 1207 G G G GNI G TG GG + N GG G+G + GGG +G Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNG 65 Query: 1208 GGAGGAGGAGGRGGKG 1223 GG G +GG G GG Sbjct: 66 GGNGNSGGGSGTGGNL 81 Score = 36.2 bits (83), Expect = 0.001 Identities = 32/81 (39%), Positives = 37/81 (45%), Gaps = 2/81 (2%) Query: 436 GKGGNGGIGGAAVTGGVAGDGGTGGKGGTGGAGGAGNDAGSTGNPGGKGGDGGIGGAGGA 495 G G G GA T G G TG G G + G+G S NP G G GI GG+ Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSG--WSSENNPWGGGSGSGIHWGGGS 60 Query: 496 GGAAGTGNGGHAGNTGDGGDG 516 G G GNG G +G GG+ Sbjct: 61 GHGNGGGNGNSGGGSGTGGNL 81 Score = 35.8 bits (82), Expect = 0.001 Identities = 29/81 (35%), Positives = 38/81 (46%), Gaps = 1/81 (1%) Query: 1167 TGTAGNGGNGGNGN-DGAVNAGTGGSGGNGGNAGGGGANGGDGGAGGAGGAGGRGGKGID 1225 +G G G N G + G +N G G G GG + G G + + GG G+G G G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 1226 GGFGGDGGNGGSNNGTGAGGN 1246 G GG GN G +GTG + Sbjct: 62 HGNGGGNGNSGGGSGTGGNLS 82 Score = 35.1 bits (80), Expect = 0.002 Identities = 32/96 (33%), Positives = 41/96 (42%), Gaps = 3/96 (3%) Query: 1061 GGEGGVGSILGGPGGNGGTGGNASATGTNGVANAGNGGKGGDGGQFGAGGNGGAGGSVTD 1120 G G+I GGP G G GG + +G + N GG G G G+G GG + Sbjct: 12 GAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGG---N 68 Query: 1121 GSAGSTAGNGGNGGNATNGTIAGQPAGGNGSAGGKG 1156 G++G +G GGN G PA AGG Sbjct: 69 GNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 35.1 bits (80), Expect = 0.002 Identities = 25/84 (29%), Positives = 33/84 (39%) Query: 1263 GDGGNGGTGGFAGFGGTAGNGGSGGTGGAGGDGGTGGDGGNGVIAGGGGTGGNGGASGAG 1322 G G G G G G +G G G G+G N GG G+G + G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 1323 GAGGTGGFAGNGNAGGNGGTGGAS 1346 G GG G +G G+ G + A+ Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAA 86 Score = 34.3 bits (78), Expect = 0.004 Identities = 35/105 (33%), Positives = 44/105 (41%), Gaps = 5/105 (4%) Query: 1233 GNGGSNNGTGAGGNGGNGGTGGVGSVGAAGGDGGNGGTGGFAGFGGTAGNGGSGGTGGAG 1292 G G + TGA GN G G G G+G + +GG +G+G G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 1293 GDGGTGGDGGNGVIAGGGGTGGNGGASGAGGAGGTGGFAGNGNAG 1337 G+GG G+ G G GTGGN A A A G + G G Sbjct: 63 GNGGGNGNSGG-----GSGTGGNLSAVAAPVAFGFPALSTPGAGG 102 Score = 34.3 bits (78), Expect = 0.004 Identities = 30/85 (35%), Positives = 38/85 (44%), Gaps = 4/85 (4%) Query: 1184 VNAGTGGSGGNGGNAGGGGANGGDGGAGGAGGAGGRGGKGIDGGFGGDGGNGGSNNGTGA 1243 ++ G G G ++ G NGG G G GGA G + G GGS +G Sbjct: 1 MSGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWG----GGSGSGIHW 56 Query: 1244 GGNGGNGGTGGVGSVGAAGGDGGNG 1268 GG G+G GG G+ G G GGN Sbjct: 57 GGGSGHGNGGGNGNSGGGSGTGGNL 81 Score = 33.9 bits (77), Expect = 0.004 Identities = 32/102 (31%), Positives = 36/102 (35%), Gaps = 1/102 (0%) Query: 580 GNGGNGGNGGNGGNAGNNSTNAPVGGEGGAGGDGGAGGAGGAANGGTAGSQ-GTGGVGGD 638 G G G N G +GN + G GG DG + GG +GS GG G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 639 GGAGGNGGGGKAGTGNSGNFGVDGEAGFSGGAGGNGGVGGAA 680 G GGNG G V F A G GG A Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 33.9 bits (77), Expect = 0.005 Identities = 32/103 (31%), Positives = 41/103 (39%) Query: 641 AGGNGGGGKAGTGNSGNFGVDGEAGFSGGAGGNGGVGGAAGANGGTGGSGGNGGDGGAGG 700 +GG+G G G ++ G G G G + G G ++ N GGSG GG G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 701 IGGAGGNGIPGTGTEPAGGTGAKGGDGGDGGAGGAGGNAGGAG 743 G GGNG G G+ G A G + AGG Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 33.5 bits (76), Expect = 0.006 Identities = 27/78 (34%), Positives = 33/78 (42%) Query: 206 GGTGGNGGNGALLIGGGGLGGAGGMGGTGGGTGGTGGNGGNGALLIGAGGVGGAGGIGGQ 265 GG G GA G GG G+G GG + G+G + N G+G GG G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 266 GTGAGGAAGAGGTGGNGG 283 G G G GG+G G Sbjct: 63 GNGGGNGNSGGGSGTGGN 80 Score = 33.5 bits (76), Expect = 0.006 Identities = 27/86 (31%), Positives = 36/86 (41%), Gaps = 5/86 (5%) Query: 542 GGAGEPGGAGGAGGAGGAAGGPGGTGGTGGNGGNGGNGGNGGNGGNGGNGGNAGNNSTNA 601 GG G G +G GGP G G GG + G+G + N GG +G+ Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGG-----ASDGSGWSSENNPWGGGSGSGIHWG 57 Query: 602 PVGGEGGAGGDGGAGGAGGAANGGTA 627 G G GG+G +GG G +A Sbjct: 58 GGSGHGNGGGNGNSGGGSGTGGNLSA 83 Score = 33.1 bits (75), Expect = 0.008 Identities = 32/110 (29%), Positives = 40/110 (36%) Query: 164 NGGAGGAGGSGGAAGGNGGNGGWLFGAGGTGGIGGTGAPGAMGGTGGNGGNGALLIGGGG 223 +GG G +G + NGG G G G+G GG G+G GG G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 224 LGGAGGMGGTGGGTGGTGGNGGNGALLIGAGGVGGAGGIGGQGTGAGGAA 273 G GG G +GGG+G G A + G GG A Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGA 111 Score = 32.8 bits (74), Expect = 0.011 Identities = 28/85 (32%), Positives = 35/85 (41%), Gaps = 2/85 (2%) Query: 1291 AGGDGGTGGDGGNGVIAGGGGTGGNGGASGAGGAGGTGGFAGNGNAGGNGGTGGASEDGD 1350 +GGDG G G GG G GGA G++ N G G G G Sbjct: 2 SGGDGR--GHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGG 59 Query: 1351 NGNAGSGATGGTGGNGGTGGDGGAA 1375 +G+ G G +GG GTGG+ A Sbjct: 60 SGHGNGGGNGNSGGGSGTGGNLSAV 84 Score = 32.8 bits (74), Expect = 0.012 Identities = 28/83 (33%), Positives = 31/83 (37%) Query: 1299 GDGGNGVIAGGGGTGGNGGASGAGGAGGTGGFAGNGNAGGNGGTGGASEDGDNGNAGSGA 1358 G G G G T GN G G G G+G + N GG S G + GSG Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 1359 TGGTGGNGGTGGDGGAAGLGGVA 1381 G G GG G L VA Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVA 85 Score = 32.4 bits (73), Expect = 0.013 Identities = 31/111 (27%), Positives = 46/111 (41%), Gaps = 7/111 (6%) Query: 784 GKGGQGGSGGTGGSGAPIGGGAGGTGGSGGHAGKGGAGGIGAQGTTITVPGNGGNAGDGG 843 G G+G + G + I GG G G GG G+ ++ P GG+ Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGG-------ASDGSGWSSENNPWGGGSGSGIH 55 Query: 844 NGGNAGAGGNGGSGDFGGNTTSGASGSGGNGGNAGTAGSGGAGGTGGTGLS 894 GG +G G GG+G+ GG + +G + S A + G GG +S Sbjct: 56 WGGGSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVS 106 Score = 32.4 bits (73), Expect = 0.015 Identities = 28/86 (32%), Positives = 35/86 (40%), Gaps = 6/86 (6%) Query: 1133 GGNATNGTIAGQPAGGNGSAGGKGGDGGNIAAGATGTAGNGGNGGNGNDGAVNAGTGGSG 1192 G ++T+G I G P G G G G + G G G G G+G Sbjct: 12 GAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG------HGNG 65 Query: 1193 GNGGNAGGGGANGGDGGAGGAGGAGG 1218 G GN+GGG GG+ A A A G Sbjct: 66 GGNGNSGGGSGTGGNLSAVAAPVAFG 91 Score = 32.4 bits (73), Expect = 0.016 Identities = 30/95 (31%), Positives = 36/95 (37%), Gaps = 1/95 (1%) Query: 1287 GTGGAGGDGGTGGDGGNGVIAGGGGTGGNGGASGAGGAGGTGGFAGNGNAGGNGGTGGAS 1346 G G G + G GN + G G G GGAS G G G+ G GG+ Sbjct: 3 GGDGRGHNTGAHSTSGN-INGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 1347 EDGDNGNAGSGATGGTGGNGGTGGDGGAAGLGGVA 1381 GN SG GTGGN A G ++ Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALS 96 Score = 32.0 bits (72), Expect = 0.019 Identities = 27/83 (32%), Positives = 33/83 (39%) Query: 551 GGAGGAGGAAGGPGGTGGTGGNGGNGGNGGNGGNGGNGGNGGNAGNNSTNAPVGGEGGAG 610 GG G G G G+G + N GG G+G + G G GN G N + G GG Sbjct: 22 GGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNGNSGGGSGTGGNL 81 Query: 611 GDGGAGGAGGAANGGTAGSQGTG 633 A A G T G+ G Sbjct: 82 SAVAAPVAFGFPALSTPGAGGLA 104 Score = 32.0 bits (72), Expect = 0.020 Identities = 32/96 (33%), Positives = 36/96 (37%), Gaps = 2/96 (2%) Query: 733 GGAGGNAGGAGGQGGNAGQGGAGGAGGNAVIPGDGVGKAPHGDAGGSGGDGGKGGQGGSG 792 G G+ GA GN G G G G G + GGSG GG G G Sbjct: 4 GDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHG 63 Query: 793 GTGGSGAPIGGGAGGTGGSGGHAGKGGAGGIGAQGT 828 GG+G GG GTGG+ A G A T Sbjct: 64 NGGGNGN--SGGGSGTGGNLSAVAAPVAFGFPALST 97 Score = 32.0 bits (72), Expect = 0.020 Identities = 28/79 (35%), Positives = 38/79 (48%) Query: 1223 GIDGGFGGDGGNGGSNNGTGAGGNGGNGGTGGVGSVGAAGGDGGNGGTGGFAGFGGTAGN 1282 G DG G + S N G G GG GS ++ + GG+G +GG +G+ Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 1283 GGSGGTGGAGGDGGTGGDG 1301 G GG G +GG GTGG+ Sbjct: 63 GNGGGNGNSGGGSGTGGNL 81 Score = 32.0 bits (72), Expect = 0.021 Identities = 31/106 (29%), Positives = 37/106 (34%), Gaps = 5/106 (4%) Query: 618 AGGAANGGTAGSQGTGGV--GGDGGAGGNGGGGKAGTGNSGNFGVDGEAGFSGGAGGNGG 675 +GG G G+ T G GG G G GG +S N + G SG GG Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSEN---NPWGGGSGSGIHWGG 58 Query: 676 VGGAAGANGGTGGSGGNGGDGGAGGIGGAGGNGIPGTGTEPAGGTG 721 G G GG+G G + G P T AGG Sbjct: 59 GSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 32.0 bits (72), Expect = 0.021 Identities = 30/86 (34%), Positives = 40/86 (46%), Gaps = 6/86 (6%) Query: 121 GANATTPGGNGGDGGWLFGSGGNGAPGAAGQSGGNGGSAGLWGNGGAGGAGGSGGAAGGN 180 GA++T+ NGG G G G + G + ++ GG +G G GGSG GG Sbjct: 12 GAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSG----SGIHWGGGSGHGNGGG 67 Query: 181 GGNGGWLFGAGGTGGIGGTGAPGAMG 206 GN G G+G G + AP A G Sbjct: 68 NGNSGG--GSGTGGNLSAVAAPVAFG 91 Score = 31.6 bits (71), Expect = 0.023 Identities = 29/110 (26%), Positives = 36/110 (32%) Query: 667 SGGAGGNGGVGGAAGANGGTGGSGGNGGDGGAGGIGGAGGNGIPGTGTEPAGGTGAKGGD 726 SGG G G + + GG G G GGA G P G +G G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 727 GGDGGAGGAGGNAGGAGGQGGNAGQGGAGGAGGNAVIPGDGVGKAPHGDA 776 G+GG G G G GG A G + G+ + A Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGA 111 Score = 31.2 bits (70), Expect = 0.036 Identities = 28/105 (26%), Positives = 36/105 (34%), Gaps = 6/105 (5%) Query: 677 GGAAGANGGTGGSGGNGGDGGAGGIGGAGGNGIPGTGTEPAGGTGAKGGDGGDGGAGGAG 736 G G N G + GN G G G G + G +E GG G G Sbjct: 4 GDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSE------NNPWGGGSGSGIHWG 57 Query: 737 GNAGGAGGQGGNAGQGGAGGAGGNAVIPGDGVGKAPHGDAGGSGG 781 G +G G G GG+G G + + P G+GG Sbjct: 58 GGSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG 102 Score = 31.2 bits (70), Expect = 0.037 Identities = 31/77 (40%), Positives = 36/77 (46%), Gaps = 7/77 (9%) Query: 565 GTGGTGGNGGNGG-----NGGNGGNGGNGGNGGNAGNNSTNAPVGGEGGAGGD--GGAGG 617 G G G N G NGG G G GG +G +S N P GG G+G GG+G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 618 AGGAANGGTAGSQGTGG 634 G NG + G GTGG Sbjct: 63 GNGGGNGNSGGGSGTGG 79 Score = 30.8 bits (69), Expect = 0.046 Identities = 32/102 (31%), Positives = 41/102 (40%), Gaps = 2/102 (1%) Query: 314 GTGGKGGQGGDGGTGGAGGAGPVLFGHGGAGGMGGQGGTGGMGGAGGDGTTVIAAGTGGE 373 G G+G G T G GP G GG G + GG G+ + G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 374 GGTGGAAGAGGAAGARGALTS--GGLAGGVGAGGTGGTGGTG 413 G GG +GG +G G L++ +A G A T G GG Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv3508	cloacin	38	3e-04	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 38.2 bits (88), Expect = 3e-04 Identities = 36/107 (33%), Positives = 41/107 (38%), Gaps = 4/107 (3%) Query: 1782 GGAGGLGGGGGTGGTNGNGGLGGGGGNGGAGGAGGTPTGSGTEGTGGDGGDAGAGGNGGS 1841 G G G + N NGG G G GGA G + + G G G GG+G Sbjct: 4 GDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHG 63 Query: 1842 ATGVGNGGNGGDGGNGGDGGNGAPGGFGGGA----GAGGLGGSGAGG 1884 G GG G G AP FG A GAGGL S + G Sbjct: 64 NGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAG 110 Score = 37.8 bits (87), Expect = 5e-04 Identities = 33/79 (41%), Positives = 39/79 (49%), Gaps = 3/79 (3%) Query: 923 GQGGQGGLGGASTTSINANGGAGGNGGTGGKGGAGGAGTLGV---GGSGGTGGDGGDAGS 979 G G+G GA +TS N NGG G G GG G + GGSG GG +G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 980 GGGGGFGGAAGKAGGGGNG 998 G GGG G + G +G GGN Sbjct: 63 GNGGGNGNSGGGSGTGGNL 81 Score = 37.8 bits (87), Expect = 5e-04 Identities = 33/79 (41%), Positives = 39/79 (49%), Gaps = 3/79 (3%) Query: 1124 GQGGQGGLGGASTTSINANGGAGGNGGTGGKGGAGGAGTLGV---GGSGGTGGDGGDAGS 1180 G G+G GA +TS N NGG G G GG G + GGSG GG +G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 1181 GGGGGFGGAAGKAGGGGNG 1199 G GGG G + G +G GGN Sbjct: 63 GNGGGNGNSGGGSGTGGNL 81 Score = 37.4 bits (86), Expect = 7e-04 Identities = 31/79 (39%), Positives = 40/79 (50%), Gaps = 2/79 (2%) Query: 538 GAGGAGGNTGVGGTNGS--GGQGGTGGAGGAGGAGGVGADNPTGIGGTGGTGGKGGAGGA 595 G G G NTG T+G+ GG G G GGA G ++N GG+G GG G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 596 GGQGGSSGAGGTNGSGGAG 614 G GG+ +GG +G+GG Sbjct: 63 GNGGGNGNSGGGSGTGGNL 81 Score = 36.6 bits (84), Expect = 9e-04 Identities = 38/119 (31%), Positives = 46/119 (38%), Gaps = 5/119 (4%) Query: 1094 GAAGNGGNGGAGGAGGAGDNNFNGGQGGAGGQGGQGGLGGASTTSINANGGAGGNGGTGG 1153 G G G N GA G N NGG G G GG G S+ + GG+G GG Sbjct: 3 GGDGRGHNTGAHSTSG----NINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGG 58 Query: 1154 KGGAGGAGTLGVGGSGGTGGDGGDAGSGGGGGFGGAAGKAGGGGNGGVGGDGGEGASGL 1212 G G G G G GG+G G + FG A G G V G ++ + Sbjct: 59 GSGHGNGGGNGNSG-GGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAAI 116 Score = 35.1 bits (80), Expect = 0.003 Identities = 36/104 (34%), Positives = 41/104 (39%), Gaps = 5/104 (4%) Query: 893 GAAGNGGNGGAGGAGGAGDNNFNGGQGGAGGQGGQGGLGGASTTSINANGGAGGNGGTGG 952 G G G N GA G N NGG G G GG G S+ + GG+G GG Sbjct: 3 GGDGRGHNTGAHSTSG----NINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGG 58 Query: 953 KGGAGGAGTLGVGGSGGTGGDGGDAGSGGGGGFGGAAGKAGGGG 996 G G G G G GG+G G + FG A G G Sbjct: 59 GSGHGNGGGNGNSG-GGSGTGGNLSAVAAPVAFGFPALSTPGAG 101 Score = 34.7 bits (79), Expect = 0.004 Identities = 32/108 (29%), Positives = 40/108 (37%), Gaps = 1/108 (0%) Query: 709 GAGGEGGAGGNSGVGGTNGSGGAGGAGGKGGTGGAGGSGADNPTGAGFAGGAGGTGGAAG 768 G G G G G G G G G + G+G S +NP G G G GG+ Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 769 AGGAGGATGTGGTGGVVGATGSAGIGGAGGRGGDGGDGASGLGLGLSG 816 G G GG+G G + A G GA GL + +S Sbjct: 63 GNGGGNGNSGGGSGT-GGNLSAVAAPVAFGFPALSTPGAGGLAVSISA 109 Score = 34.7 bits (79), Expect = 0.004 Identities = 30/83 (36%), Positives = 35/83 (42%) Query: 1410 GGAGGRGGDGGAGDSGALSQDGNGFAGGQGGQGGVGGNAGAGGINGAGGTGGTGGAGGDG 1469 GG G G GGA D S + N + GG G GG +G G G G +GG G GG+ Sbjct: 22 GGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNGNSGGGSGTGGNL 81 Query: 1470 QNGTTGVASEGGAGGQGGDGGQG 1492 VA A G GG Sbjct: 82 SAVAAPVAFGFPALSTPGAGGLA 104 Score = 34.7 bits (79), Expect = 0.004 Identities = 30/83 (36%), Positives = 35/83 (42%) Query: 1615 GGAGGRGGDGGAGDSGALSQDGNGFAGGQGGQGGVGGNAGAGGINGAGGTGGTGGAGGDG 1674 GG G G GGA D S + N + GG G GG +G G G G +GG G GG+ Sbjct: 22 GGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNGNSGGGSGTGGNL 81 Query: 1675 QNGTTGVASEGGAGGQGGDGGQG 1697 VA A G GG Sbjct: 82 SAVAAPVAFGFPALSTPGAGGLA 104 Score = 34.7 bits (79), Expect = 0.004 Identities = 31/97 (31%), Positives = 37/97 (38%) Query: 126 GGAGGAGGLLFGNGGAGAAGAPGQAGGPGGPAGLWGNGGPGGAGGSGGGTGGAGGAGGWL 185 GG G G + G+G + GG G WG G G GG G +GG G GG L Sbjct: 22 GGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNGNSGGGSGTGGNL 81 Query: 186 FGVGGAGGVGGAGGGTGGAGGPGGLIWGGGGAGGVGG 222 V G T GAGG I G + + Sbjct: 82 SAVAAPVAFGFPALSTPGAGGLAVSISAGALSAAIAD 118 Score = 33.9 bits (77), Expect = 0.006 Identities = 34/104 (32%), Positives = 38/104 (36%), Gaps = 1/104 (0%) Query: 1171 TGGDGGDAGSGGGGGFGGAAGKAGGGGNGGVGGDGGEGASGLGLGLSGFDGGQGGQGGAG 1230 +GGDG +G G G G G GG G G G S G G GG Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGG-GASDGSGWSSENNPWGGGSGSGIHWGGGS 60 Query: 1231 GSAGAGGINGAGGAGGTGGAGGDGAPATLIGGPDGGDGGQGGIG 1274 G GG +GG GTGG A G P G GG+ Sbjct: 61 GHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 33.9 bits (77), Expect = 0.006 Identities = 35/102 (34%), Positives = 39/102 (38%) Query: 759 GAGGTGGAAGAGGAGGATGTGGTGGVVGATGSAGIGGAGGRGGDGGDGASGLGLGLSGFD 818 G G G GA G G TG VG S G G + GG SG+ G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 819 GGQGGQGGAGGSAGAGGINGAGGAGGNGGDGGDGATGAAGLG 860 G GG G +GG +G GG A A G GA GL Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 33.5 bits (76), Expect = 0.008 Identities = 33/101 (32%), Positives = 39/101 (38%) Query: 1435 AGGQGGQGGVGGNAGAGGINGAGGTGGTGGAGGDGQNGTTGVASEGGAGGQGGDGGQGGI 1494 +GG G G ++ +G ING G GG DG ++ GG G G G G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 1495 GGAGGNAGFGAGVPGDGGIGGTGGAGGAGGAGADGDPSIDG 1535 G GG G G G GG A A G A P G Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG 102 Score = 33.5 bits (76), Expect = 0.008 Identities = 33/101 (32%), Positives = 39/101 (38%) Query: 1640 AGGQGGQGGVGGNAGAGGINGAGGTGGTGGAGGDGQNGTTGVASEGGAGGQGGDGGQGGI 1699 +GG G G ++ +G ING G GG DG ++ GG G G G G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 1700 GGAGGNAGFGAGVPGDGGIGGTGGAGGAGGAGADGDPSIDG 1740 G GG G G G GG A A G A P G Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG 102 Score = 33.5 bits (76), Expect = 0.009 Identities = 35/113 (30%), Positives = 50/113 (44%), Gaps = 2/113 (1%) Query: 594 GAGGQGGSSGAGGTNGS--GGAGGTGGQGGAGGAGGAGADNPTGIGGAGGTGGTGGAAGA 651 G G+G ++GA T+G+ GG G G GGA G ++N GG+G GG +G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 652 GGAGGAIGTGGTGGAVGSVGNAGIGGTGGTGGVGGAGGAGAAAAAGSSATGGA 704 G GG +GG G G++ G + G G A + + A A Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAA 115 Score = 33.1 bits (75), Expect = 0.012 Identities = 25/85 (29%), Positives = 31/85 (36%) Query: 1730 AGADGDPSIDGGQGGAGGHGGQGGKGGLNSTGLASAASGDGGNGGAGGAGGNGGAGGLGG 1789 +G DG G +G G G+ + N GG+G GG G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 1790 GGGTGGTNGNGGLGGGGGNGGAGGA 1814 G GG +GG G GGN A A Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAA 86 Score = 33.1 bits (75), Expect = 0.012 Identities = 29/88 (32%), Positives = 34/88 (38%), Gaps = 4/88 (4%) Query: 656 GAIGTGGTGGAVGSVGNAGIGGTGGTGGVGGAGGAGAAAAAGSSATGGAGFAGGAGGEGG 715 G G G GA + GN GG G+G GGA + S G +G GG Sbjct: 3 GGDGRGHNTGAHSTSGNI----NGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGG 58 Query: 716 AGGNSGVGGTNGSGGAGGAGGKGGTGGA 743 G+ GG SGG G GG A Sbjct: 59 GSGHGNGGGNGNSGGGSGTGGNLSAVAA 86 Score = 33.1 bits (75), Expect = 0.013 Identities = 32/82 (39%), Positives = 38/82 (46%), Gaps = 2/82 (2%) Query: 1821 SGTEGTGGDGGDAGAGGN-GGSATGVGNGGNGGDGGNGGDGGNGAPGGFGGGAGAGGLGG 1879 SG +G G + G GN G TG+G GG DG N GG G G GG G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 1880 SGAGGGTDGDDGNGGSPGTDGS 1901 G GGG +G+ G G G + S Sbjct: 62 HGNGGG-NGNSGGGSGTGGNLS 82 Score = 32.8 bits (74), Expect = 0.015 Identities = 27/80 (33%), Positives = 32/80 (40%) Query: 1807 GNGGAGGAGGTPTGSGTEGTGGDGGDAGAGGNGGSATGVGNGGNGGDGGNGGDGGNGAPG 1866 G G G G + SG G G G G + GS N GG G+G G G+ Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 1867 GFGGGAGAGGLGGSGAGGGT 1886 G GGG G G G G + Sbjct: 63 GNGGGNGNSGGGSGTGGNLS 82 Score = 32.8 bits (74), Expect = 0.016 Identities = 37/106 (34%), Positives = 43/106 (40%), Gaps = 4/106 (3%) Query: 569 AGGVGADNPTGIGGTGGTGGKGGAGGAGGQGGSSGAG--GTNGSGGAGGTGGQGGAGGAG 626 +GG G + TG T G G G G G S G+G N G G G GG+G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 627 GAGADNPTGIGGAGGTGGTGGAAGAGGAGG--AIGTGGTGGAVGSV 670 GG GTGG A A A G A+ T G GG S+ Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSI 107 Score = 32.4 bits (73), Expect = 0.018 Identities = 25/86 (29%), Positives = 33/86 (38%) Query: 1738 IDGGQGGAGGHGGQGGKGGLNSTGLASAASGDGGNGGAGGAGGNGGAGGLGGGGGTGGTN 1797 + GG G G G +N G +G + N GG G G GG + Sbjct: 1 MSGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGS 60 Query: 1798 GNGGLGGGGGNGGAGGAGGTPTGSGT 1823 G+G GG G +GG G GG + Sbjct: 61 GHGNGGGNGNSGGGSGTGGNLSAVAA 86 Score = 32.0 bits (72), Expect = 0.024 Identities = 36/117 (30%), Positives = 46/117 (39%), Gaps = 2/117 (1%) Query: 556 GQGGTGGAGGAGGAGGVGADNPTGIGGTGG-TGGKGGAGGAGGQGGSSGAGGTNGSGGAG 614 G G G GA G PTG+G GG + G G + GG SG+G G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 615 GTGGQGGAGGAGGAGADNPTGIGGAGGTGGTGGAA-GAGGAGGAIGTGGTGGAVGSV 670 G GG G G G N + + G + GAGG +I G A+ + Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAAIADI 119 Score = 32.0 bits (72), Expect = 0.025 Identities = 36/116 (31%), Positives = 46/116 (39%), Gaps = 3/116 (2%) Query: 1416 GGDGGAGDSGALSQDGNGFAGGQGGQGGVGGNAGAGGINGAGGTGGTGGAGGDGQNGTTG 1475 GGDG ++GA S GN G G G G + G+G + GG G+G G+ Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 1476 VASEGGAGGQGGDGGQGGIGGAGGNAGFG---AGVPGDGGIGGTGGAGGAGGAGAD 1528 G GG G G + FG PG GG+ + AG A AD Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAAIAD 118 Score = 32.0 bits (72), Expect = 0.025 Identities = 36/116 (31%), Positives = 46/116 (39%), Gaps = 3/116 (2%) Query: 1621 GGDGGAGDSGALSQDGNGFAGGQGGQGGVGGNAGAGGINGAGGTGGTGGAGGDGQNGTTG 1680 GGDG ++GA S GN G G G G + G+G + GG G+G G+ Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 1681 VASEGGAGGQGGDGGQGGIGGAGGNAGFG---AGVPGDGGIGGTGGAGGAGGAGAD 1733 G GG G G + FG PG GG+ + AG A AD Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAAIAD 118 Score = 32.0 bits (72), Expect = 0.029 Identities = 36/107 (33%), Positives = 45/107 (42%), Gaps = 4/107 (3%) Query: 970 TGGDGGDAGSGGGGGFGGAAGKAGGGGNGGRGGDGGDGASGLGLGLSGFDGGQGGQGGAG 1029 +GGDG +G G G G G GG DG SG + + GG G G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDG----SGWSSENNPWGGGSGSGIHWG 57 Query: 1030 GSAGAGGINGAGGAGGNGGDGGDGATGAAGLGDNGGVGGDGGAGGAA 1076 G +G G G G +GG G GG+ + AA + GAGG A Sbjct: 58 GGSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 32.0 bits (72), Expect = 0.030 Identities = 29/87 (33%), Positives = 34/87 (39%), Gaps = 3/87 (3%) Query: 483 GAAGTGGTGGVVGAAGKAGIGGTGGQGGAGGAGSAGTDATATGATGGTGFSGGAGGAGGA 542 G G G G +G G TG G G + +G + GG+G GG G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 543 GGNTGVGGTNGSGGQGGTGGAGGAGGA 569 G GG SGG GTGG A A Sbjct: 63 GNG---GGNGNSGGGSGTGGNLSAVAA 86 Score = 31.6 bits (71), Expect = 0.039 Identities = 42/117 (35%), Positives = 50/117 (42%), Gaps = 5/117 (4%) Query: 873 GAAGNGGNAGVGLTAKAGDGGAAGNGGNGGAG-GAGGAGDNNFNGGQGGAGGQGGQGGLG 931 G G G N G T+ +GG G G GGA G+G + +NN GG G+G G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 932 GASTTSINANGGAGGNGGTGGKGGAGGAGTLGVGGSGGTGGDGGDAGSGGGGGFGGA 988 G NG +GG GTGG A A + T G GG A S G A Sbjct: 63 GNG----GGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAA 115 Score = 31.6 bits (71), Expect = 0.039 Identities = 42/117 (35%), Positives = 50/117 (42%), Gaps = 5/117 (4%) Query: 1074 GAAGNGGNAGVGLTAKAGDGGAAGNGGNGGAG-GAGGAGDNNFNGGQGGAGGQGGQGGLG 1132 G G G N G T+ +GG G G GGA G+G + +NN GG G+G G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 1133 GASTTSINANGGAGGNGGTGGKGGAGGAGTLGVGGSGGTGGDGGDAGSGGGGGFGGA 1189 G NG +GG GTGG A A + T G GG A S G A Sbjct: 63 GNG----GGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAA 115
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv3511	cloacin	40	2e-05	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 40.1 bits (93), Expect = 2e-05 Identities = 31/89 (34%), Positives = 33/89 (37%) Query: 270 GAGGTGGNAAWLLGGGGTGGAGGIGGGNGGHGGNGGWLLGNGGNGGLGGDGDGGTGGGHG 329 G G G N G G G GG GW N GG G G GG Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 330 GNGGNPGWLLGTAGGGGNGGAGSTGTAGG 358 GNGG G G +G GGN A + A G Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFG 91 Score = 37.0 bits (85), Expect = 2e-04 Identities = 31/79 (39%), Positives = 36/79 (45%), Gaps = 1/79 (1%) Query: 237 GAGGIGGGSGGAGGNGGWLLGNGGIGGAGGTGGGAGGTGGNAAWLLGGG-GTGGAGGIGG 295 G G G +G +G G G+G GG G+G + N W G G G GG G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 296 GNGGHGGNGGWLLGNGGNG 314 GNGG GN G G GGN Sbjct: 63 GNGGGNGNSGGGSGTGGNL 81 Score = 37.0 bits (85), Expect = 3e-04 Identities = 35/115 (30%), Positives = 46/115 (40%), Gaps = 2/115 (1%) Query: 583 AGAGGTGFTQGADGNAGNGGDGGVGGNGGNGADNTTTAAAGTTGGAGGAGGAGGTGGAAG 642 +G G G GA +GN G G G GA + + ++ GG+G GG +G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 643 TGTGGQQGNGGNGGNGGTGGKGGTGGAGMNSLDPLLAAQDGGQGGTGGTGGNAGA 697 G GG GN G G GTGG A + P L+ G + G A Sbjct: 62 HGNGGGNGNSGGG--SGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSA 114 Score = 36.6 bits (84), Expect = 3e-04 Identities = 28/73 (38%), Positives = 33/73 (45%), Gaps = 5/73 (6%) Query: 233 GGHGGAGGIGGGSGGAGGNGGWLLGNGGIGGAGGTGGGAGGTGGNAAWLLGGGGTGGAGG 292 G H +G I GG G G GG + G G + GG+G W GGG G GG Sbjct: 12 GAHSTSGNINGGPTGLGVGGG---ASDGSGWSSENNPWGGGSGSGIHW--GGGSGHGNGG 66 Query: 293 IGGGNGGHGGNGG 305 G +GG G GG Sbjct: 67 GNGNSGGGSGTGG 79 Score = 36.2 bits (83), Expect = 4e-04 Identities = 28/84 (33%), Positives = 31/84 (36%), Gaps = 5/84 (5%) Query: 244 GSGGAGGNGGWLLGNGGIGGAGGTGGGAGGTGGNAAWLLGGGGTGGAGGIGGGNGGHGGN 303 G G G N G +G I G G GG + W GG G G GG G+ Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 304 GGWLLGNGGNGGLGGDGDGGTGGG 327 GNGG G G G G G Sbjct: 63 -----GNGGGNGNSGGGSGTGGNL 81 Score = 35.8 bits (82), Expect = 6e-04 Identities = 35/106 (33%), Positives = 43/106 (40%), Gaps = 6/106 (5%) Query: 378 GAGAGGHGGTGGAGGAGVNGGGAGGAGGAGGNGGAGGQAALLFGRGGTGGAGGYGGDGGG 437 GA + GG G GV GG + G+G + N GG G G GG G G G Sbjct: 12 GAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGG------GSGSGIHWGGGSGHGNG 65 Query: 438 GGDGFDGTMAGLGGTGGSGGTGGDGGAPGNGGAGGAGQLLSHSGVA 483 GG+G G +G GG + G P G G +S S A Sbjct: 66 GGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGA 111 Score = 35.5 bits (81), Expect = 7e-04 Identities = 29/100 (29%), Positives = 35/100 (35%) Query: 486 SGKGGAGGTGGNGGAGSAGADAPAGSGAMGSTGFAGGAGGDGGNGGGSGASQGNGGNGGN 545 S G G G G +D S G G+G G G G G GNG +GG Sbjct: 15 STSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNGNSGGG 74 Query: 546 GGTGGKGGTGGAGMNSLDPLLAAQDGGQGGTGGTGGNAGA 585 GTGG A + P L+ G + G A Sbjct: 75 SGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSA 114 Score = 35.5 bits (81), Expect = 8e-04 Identities = 36/104 (34%), Positives = 43/104 (41%), Gaps = 3/104 (2%) Query: 193 AGGIGGGTGGAGGHAWLFGHGGTGGIGGGPGGN--GGWLLGNGGHGGAGGIGGGSGGAGG 250 +GG G G +GG G+G G G + GW N GG G G GG G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 251 NGGWLLGNGGIGGAGGTGGGAGGTGGNAAWLLGGGGTGGAGGIG 294 +G GNG GG GTGG A+ T GAGG+ Sbjct: 62 HGNG-GGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 35.1 bits (80), Expect = 0.001 Identities = 30/81 (37%), Positives = 35/81 (43%), Gaps = 7/81 (8%) Query: 289 GAGGIGGGNGGHGGNGGWLLGNGGNGGLGGDGDGGTGGG----HGGNGGNPGWLLGTAGG 344 G G G G H +G NGG GLG G G G + GG G + GG Sbjct: 3 GGDGRGHNTGAHSTSGNI---NGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGG 59 Query: 345 GGNGGAGSTGTAGGGSGGTGG 365 G+G G G +GGGSG G Sbjct: 60 SGHGNGGGNGNSGGGSGTGGN 80 Score = 33.9 bits (77), Expect = 0.002 Identities = 35/104 (33%), Positives = 42/104 (40%), Gaps = 9/104 (8%) Query: 176 GNGGNAGWLYGRGGV-GGAGGIGGGTGGAGGHAWLFGH---GGTGGIGGGPGGNGGWLLG 231 G G N G G + GG G+G G G + G W + GG G G GG G Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG---- 61 Query: 232 NGGHGGAGGIGGGSGGAGGNGGWLLGNGGIGGAGGTGGGAGGTG 275 G+GG G GG G GGN + G + GAGG Sbjct: 62 -HGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 33.9 bits (77), Expect = 0.003 Identities = 28/95 (29%), Positives = 35/95 (36%) Query: 310 NGGNGGLGGDGDGGTGGGHGGNGGNPGWLLGTAGGGGNGGAGSTGTAGGGSGGTGGDGGT 369 N G G+ +GG G G G + G + GG+GS GGGSG G G Sbjct: 10 NTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNG 69 Query: 370 GGRGGLLMGAGAGGHGGTGGAGGAGVNGGGAGGAG 404 GG G G ++ GAGG Sbjct: 70 NSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 33.5 bits (76), Expect = 0.003 Identities = 28/85 (32%), Positives = 32/85 (37%), Gaps = 6/85 (7%) Query: 490 GAGGTGGNGGAGSAGADAPAGSGAMGSTGFAGGAGGDG------GNGGGSGASQGNGGNG 543 G G G N GA S + G +G G A G G G GSG G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 544 GNGGTGGKGGTGGAGMNSLDPLLAA 568 GNGG G G G +L + A Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAP 87 Score = 33.5 bits (76), Expect = 0.003 Identities = 33/134 (24%), Positives = 45/134 (33%), Gaps = 13/134 (9%) Query: 376 LMGAGAGGHGGTGGAGGAGVNGGGAGGAGGAGGNGGAGGQAALLFGRGGTGGAGGYGGDG 435 + G GH + +NGG G G G + G+G + GG+G +GG Sbjct: 1 MSGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGS 60 Query: 436 GGGGDGFDGTMAGLGGTGGSGGTGGDGGAPGNGGAGGAGQLLSHSGVAGASGKGGAGGTG 495 G G GG G G G G + A + G GG + Sbjct: 61 GHGN-------------GGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSI 107 Query: 496 GNGGAGSAGADAPA 509 G +A AD A Sbjct: 108 SAGALSAAIADIMA 121 Score = 32.8 bits (74), Expect = 0.005 Identities = 34/114 (29%), Positives = 39/114 (34%), Gaps = 14/114 (12%) Query: 527 GGNGGGSGASQGNGGNGGNGGTGGKGGTGGAGMNSLDPLLAAQDGGQGGTGGTGGNAGAG 586 GG+G G + NGG G G GGA G G + G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGA------------SDGSGWSSENNPWGGGS 50 Query: 587 GTGFTQGADGNAGNGGDGGVGGNGGNGADNTTTAAAGTTGG--AGGAGGAGGTG 638 G+G G GNGG G G G N + AA G A GAGG Sbjct: 51 GSGIHWGGGSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 32.0 bits (72), Expect = 0.009 Identities = 32/106 (30%), Positives = 36/106 (33%), Gaps = 10/106 (9%) Query: 421 GRGGTGGAGGYGGDGGGGGDGFDGTMAGLGGTGGSGGTGGDGGAPGNGGAGGAGQLLSHS 480 GRG GA G+ GG G G G + G G + N GG H Sbjct: 6 GRGHNTGAHSTSGNINGGPTG---------LGVGGGASDGSGWSSENNPWGGGSGSGIHW 56 Query: 481 GVAGASGKGGAGGTGGNGGAGSAGADAPAGSGAMGSTGFAG-GAGG 525 G G GG G G G A A A G + GAGG Sbjct: 57 GGGSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG 102 Score = 31.6 bits (71), Expect = 0.013 Identities = 33/101 (32%), Positives = 35/101 (34%), Gaps = 2/101 (1%) Query: 219 GGGPGGNGGWLLGNGGHGGAGGIGGGSGGAGGNGGWLLGNGGIGGAGGTGGGAGGTGG-- 276 G G G N G +G G G GGA GW N GG G+G GG G Sbjct: 4 GDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHG 63 Query: 277 NAAWLLGGGGTGGAGGIGGGNGGHGGNGGWLLGNGGNGGLG 317 N GG G GG G L G GGL Sbjct: 64 NGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 31.2 bits (70), Expect = 0.016 Identities = 27/96 (28%), Positives = 33/96 (34%), Gaps = 8/96 (8%) Query: 352 STGTAGGGSGGTGGDGGTGGRGGLLMGAGAGGHGGTGGAGGAGVNGGGAGGAGGAGGNGG 411 S G G + G G G +G G G G+G + GGG+G GG G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 412 AGGQAALLFGRGGTGGAGGYGGDGGGGGDGFDGTMA 447 G GG G G G GG +A Sbjct: 62 HGN--------GGGNGNSGGGSGTGGNLSAVAAPVA 89 Score = 30.8 bits (69), Expect = 0.021 Identities = 22/77 (28%), Positives = 29/77 (37%) Query: 572 GQGGTGGTGGNAGAGGTGFTQGADGNAGNGGDGGVGGNGGNGADNTTTAAAGTTGGAGGA 631 G+G G +G G T G + G G N G + + G G G Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNG 65 Query: 632 GGAGGTGGAAGTGTGGQ 648 GG G +GG +GTG Sbjct: 66 GGNGNSGGGSGTGGNLS 82 Score = 30.5 bits (68), Expect = 0.027 Identities = 27/101 (26%), Positives = 37/101 (36%), Gaps = 17/101 (16%) Query: 494 TGGNGGAGSAGADAPAGSGAMGSTGFAGGAGGDGGNGGGSGASQGNGGNGGNGGTGGKGG 553 +GG+G + GA + +G+ G TG G G G+G S + GG+G GG G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 554 TGGAGMNSLDPLLAAQDGGQGGTGGTGGNAGAGGTGFTQGA 594 G GG G G G + A Sbjct: 62 HGN-----------------GGGNGNSGGGSGTGGNLSAVA 85 Score = 30.5 bits (68), Expect = 0.028 Identities = 30/90 (33%), Positives = 39/90 (43%), Gaps = 13/90 (14%) Query: 345 GGNGGAGSTGTAGGGSGGTGGDGGTGGRGGLLMGAGAGG-HGGTGGAGGAGVNGGGAGGA 403 GG+G +TG GG G G GG G+G + GG G+G++ GG G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 404 GGAGGNGGAGGQAALLFGRGGTGGAGGYGG 433 G GGNG +GG G+G G Sbjct: 63 GNGGGNGNSGG------------GSGTGGN 80 Score = 30.5 bits (68), Expect = 0.029 Identities = 27/79 (34%), Positives = 29/79 (36%), Gaps = 1/79 (1%) Query: 148 GQAGGAGGSAGLLGNGGSGGAGGTGAPGGNGGNAGWLYGRGGVGGAGGIGGGTGGAGGHA 207 G G A +GG G G GG +GW GG G G GG GH Sbjct: 4 GDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHG 63 Query: 208 WLFGHGGTGGIGGGPGGNG 226 G G G G G GGN Sbjct: 64 -NGGGNGNSGGGSGTGGNL 81 Score = 29.7 bits (66), Expect = 0.048 Identities = 34/110 (30%), Positives = 38/110 (34%), Gaps = 6/110 (5%) Query: 402 GAGGAGGNGGAGGQAALLFGRGGTGGAGGYGGDGGGGGDGFDGTMAGLGGTGGSGGTGGD 461 G G G N GA + G G G GGG DG + GGSG Sbjct: 3 GGDGRGHNTGAHSTS------GNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHW 56 Query: 462 GGAPGNGGAGGAGQLLSHSGVAGASGKGGAGGTGGNGGAGSAGADAPAGS 511 GG G+G GG G SG G A G + GA A S Sbjct: 57 GGGSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVS 106 Score = 29.7 bits (66), Expect = 0.049 Identities = 27/83 (32%), Positives = 32/83 (38%), Gaps = 12/83 (14%) Query: 619 TAAAGTTGGAGGAGGAGGTGGAAGTGTGGQQGNGGNGGNGGTGGKGGTGGAGMNSLDPLL 678 T A T+G G G GG A G+G N GG G+G G G Sbjct: 11 TGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH-------- 62 Query: 679 AAQDGGQGGTGGTGGNAGAGGTG 701 G GG G +GG +G GG Sbjct: 63 ----GNGGGNGNSGGGSGTGGNL 81
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv3512	cloacin	37	5e-04	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 36.6 bits (84), Expect = 5e-04 Identities = 38/104 (36%), Positives = 44/104 (42%), Gaps = 2/104 (1%) Query: 444 GQGGAGGTGGAGAASSATNGGSGGAGGTGGDGGSGGAGGTGGAGGTGGAAGDGGQGGQGG 503 G G G GA + S NGG G G GG S G+G + GG +G G G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGG--ASDGSGWSSENNPWGGGSGSGIHWGGGS 60 Query: 504 AGGGAGGQGGAGGAGGTGGNGGNITGGTAGTAGAAGNGGAAGKG 547 G GG G +GG GTGGN + A A GA G Sbjct: 61 GHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 36.2 bits (83), Expect = 8e-04 Identities = 37/111 (33%), Positives = 47/111 (42%), Gaps = 5/111 (4%) Query: 633 GNGTGGAGGNGGGGANGGAGGAGGSGGGTGGNGGAGGDAGDAGNGGNGNGTGNGGNGGNG 692 G G + G NGG G G GG + G+G + + G G+G G G GNG Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNG 65 Query: 693 GIAGMGGNGGAGTGSGNGGNGGSGGNGGNAGMGGNSGTGSGDGGAGGNGGA 743 GGNG +G GSG GGN + G S G+G + GA Sbjct: 66 -----GGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGA 111 Score = 36.2 bits (83), Expect = 8e-04 Identities = 26/73 (35%), Positives = 31/73 (42%) Query: 588 GSGGQGGNAGGGGAGGQGGADGGSGGDGGDAGTGGNGGNGGNRNSGNGTGGAGGNGGGGA 647 G + G GG G G G G + N GG SG GG G+G GG Sbjct: 8 GHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGG 67 Query: 648 NGGAGGAGGSGGG 660 NG +GG G+GG Sbjct: 68 NGNSGGGSGTGGN 80 Score = 35.8 bits (82), Expect = 0.001 Identities = 29/78 (37%), Positives = 32/78 (41%) Query: 948 GAAGNGGNGGNAGAGGNGNGGTGGAGGIGGTGGNGGDAEPGVPPGAGGAGGAGTTGGKGG 1007 G G G N G GN NGG G G GG G + P G G G GG G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 1008 TGGNGSGTGSGGTGGDGG 1025 G G+G GG+G G Sbjct: 63 GNGGGNGNSGGGSGTGGN 80 Score = 34.7 bits (79), Expect = 0.002 Identities = 25/74 (33%), Positives = 32/74 (43%) Query: 678 GNGNGTGNGGNGGNGGIAGMGGNGGAGTGSGNGGNGGSGGNGGNAGMGGNSGTGSGDGGA 737 G+ G + NGG G+G GGA GSG G G +G+ G+G G+GG Sbjct: 8 GHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGG 67 Query: 738 GGNGGAAGTGGTGG 751 GN G G Sbjct: 68 NGNSGGGSGTGGNL 81 Score = 34.7 bits (79), Expect = 0.002 Identities = 30/78 (38%), Positives = 36/78 (46%) Query: 682 GTGNGGNGGNGGIAGMGGNGGAGTGSGNGGNGGSGGNGGNAGMGGNSGTGSGDGGAGGNG 741 G G G N G +G G G G G G + GSG + N GG SG+G GG G+G Sbjct: 4 GDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHG 63 Query: 742 GAAGTGGTGGDGGLTGTG 759 G G +GG G G Sbjct: 64 NGGGNGNSGGGSGTGGNL 81 Score = 34.7 bits (79), Expect = 0.002 Identities = 30/84 (35%), Positives = 35/84 (41%) Query: 732 SGDGGAGGNGGAAGTGGTGGDGGLTGTGGTGGSGGTGGDGGNGGNGADNTANMTAQAGGD 791 SG G G N GA T G G G G S G+G N G + + + G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 792 GGNGGDGGFGGGAGAGGGGLTAGA 815 GNGG G GG GG L+A A Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVA 85 Score = 34.3 bits (78), Expect = 0.003 Identities = 35/87 (40%), Positives = 42/87 (48%), Gaps = 3/87 (3%) Query: 658 GGGTGGNGGAGGDAGDAGNGGNGNGTGNGGNGGNGGIAGMGGNGGAGTGSGNGGNGGSGG 717 G G G N GA +G+ G G G G G + G+G + GG GSG+G + G G Sbjct: 4 GDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGG---GSGSGIHWGGGS 60 Query: 718 NGGNAGMGGNSGTGSGDGGAGGNGGAA 744 GN G GNSG GSG GG A Sbjct: 61 GHGNGGGNGNSGGGSGTGGNLSAVAAP 87 Score = 33.9 bits (77), Expect = 0.004 Identities = 31/97 (31%), Positives = 37/97 (38%) Query: 845 GNGGTGGNGGTGGTGGAGIGSLGGGTGGDGGNGGNGGTGGEGGEVGGAGGTGGAAGNGGD 904 G G G T G G LG G G G+G + GG G GG +G+G Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNG 65 Query: 905 GGTGGTGGGDGGAGGTGGTGGTGGLGDPRVGGSGGDG 941 GG G +GGG G G G P + G G Sbjct: 66 GGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG 102 Score = 33.5 bits (76), Expect = 0.005 Identities = 29/80 (36%), Positives = 32/80 (40%) Query: 420 SGGAGGAAGAGGAGGGANGTAGNGGQGGAGGTGGAGAASSATNGGSGGAGGTGGDGGSGG 479 SGG G G N G G G GG SS N GG+G GG G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 480 AGGTGGAGGTGGAAGDGGQG 499 G GG G +GG +G GG Sbjct: 62 HGNGGGNGNSGGGSGTGGNL 81 Score = 33.5 bits (76), Expect = 0.005 Identities = 24/75 (32%), Positives = 32/75 (42%) Query: 1004 GKGGTGGNGSGTGSGGTGGDGGTGGGGGNGGTGWNGGKGDTGSGGGAGDGGKAPAGGTGG 1063 G+G G S +G+ G G GGG + G+GW+ G G G+G +G G Sbjct: 6 GRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNG 65 Query: 1064 AGGDGGAGGKGGSGG 1078 G GG G G Sbjct: 66 GGNGNSGGGSGTGGN 80 Score = 33.1 bits (75), Expect = 0.006 Identities = 25/84 (29%), Positives = 30/84 (35%) Query: 600 GAGGQGGADGGSGGDGGDAGTGGNGGNGGNRNSGNGTGGAGGNGGGGANGGAGGAGGSGG 659 G G+G G G G G GG + G+G GGG+ G GGSG Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 660 GTGGNGGAGGDAGDAGNGGNGNGT 683 G GG G G G + Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAA 86 Score = 32.8 bits (74), Expect = 0.008 Identities = 35/109 (32%), Positives = 41/109 (37%), Gaps = 1/109 (0%) Query: 410 GNGGQGGQGGSGGAGGAAGAGGAGGGANGTAGNG-GQGGAGGTGGAGAASSATNGGSGGA 468 G G+G G+ G G G G G A +G G G G+ S GG G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 469 GGTGGDGGSGGAGGTGGAGGTGGAAGDGGQGGQGGAGGGAGGQGGAGGA 517 G GG+G SGG GTGG A G G G + GA Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGA 111 Score = 32.8 bits (74), Expect = 0.008 Identities = 35/101 (34%), Positives = 40/101 (39%), Gaps = 2/101 (1%) Query: 565 AGGDGGAGGTGGDRTVGG--GTVPAGSGGQGGNAGGGGAGGQGGADGGSGGDGGDAGTGG 622 +GGDG TG T G G G G + G G + GGSG G G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 623 NGGNGGNRNSGNGTGGAGGNGGGGANGGAGGAGGSGGGTGG 663 +G GGN NSG G+G G A G S G GG Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG 102 Score = 32.8 bits (74), Expect = 0.008 Identities = 28/86 (32%), Positives = 37/86 (43%), Gaps = 2/86 (2%) Query: 589 SGGQGGNAGGGGAGGQGGADGGSGGDGGDAGTGGNGGNGGNRNSGNGTGGAGGNGGGGAN 648 SGG G G G +GG G G G G + G+G + + GG+G G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGV--GGGASDGSGWSSENNPWGGGSGSGIHWGGG 59 Query: 649 GGAGGAGGSGGGTGGNGGAGGDAGDA 674 G G GG+G GG+G G + A Sbjct: 60 SGHGNGGGNGNSGGGSGTGGNLSAVA 85 Score = 32.4 bits (73), Expect = 0.010 Identities = 27/84 (32%), Positives = 30/84 (35%) Query: 878 GNGGTGGEGGEVGGAGGTGGAAGNGGDGGTGGTGGGDGGAGGTGGTGGTGGLGDPRVGGS 937 G G G G +G G G GG G G G G G+ G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 938 GGDGGTGGSGGAAGNGGNGGNAGA 961 G GG G SGG +G GGN A Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAA 86 Score = 32.4 bits (73), Expect = 0.012 Identities = 28/70 (40%), Positives = 29/70 (41%) Query: 844 GGNGGTGGNGGTGGTGGAGIGSLGGGTGGDGGNGGNGGTGGEGGEVGGAGGTGGAAGNGG 903 G T GN G TG G G+G N GG G G GG G G GNG Sbjct: 11 TGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNGN 70 Query: 904 DGGTGGTGGG 913 GG GTGG Sbjct: 71 SGGGSGTGGN 80 Score = 32.0 bits (72), Expect = 0.014 Identities = 32/102 (31%), Positives = 41/102 (40%) Query: 120 GGKGGNGGIGAAGTTGPVGTGASGGTGGSGGAGGTGGDGGAANGGTAGAGGAGGNGGKGG 179 GG G GA T+G + G +G G G + G+G G G GG G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 180 DGGAGVTSSTAGNSGGAGGSGGKGGDAGAGGAGATPGANGIA 221 G G +S G+ G S A A +TPGA G+A Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 32.0 bits (72), Expect = 0.015 Identities = 27/83 (32%), Positives = 32/83 (38%), Gaps = 1/83 (1%) Query: 663 GNGGAGGDAGDAGNGGNGNGTGNGGNGGNGGIAGMG-GNGGAGTGSGNGGNGGSGGNGGN 721 G G G + G GN NG G G G G G + G G+G GG G+ Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 722 AGMGGNSGTGSGDGGAGGNGGAA 744 GGN +G G G G A Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVA 85 Score = 31.6 bits (71), Expect = 0.017 Identities = 26/80 (32%), Positives = 33/80 (41%) Query: 242 GAGDGGHGGTGAAGGNGGTGGAGGSGIDGVGGGTGGTGGNGGNGAIGGAGGDAGGSGNSG 301 G G G + G + GN G G G G+G + N G G+G GG G Sbjct: 4 GDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHG 63 Query: 302 GNGGIGGKGGNAGAGGAAGS 321 GG G GG +G GG + Sbjct: 64 NGGGNGNSGGGSGTGGNLSA 83 Score = 31.6 bits (71), Expect = 0.021 Identities = 38/115 (33%), Positives = 46/115 (40%), Gaps = 3/115 (2%) Query: 700 NGGAGTGSGNGGNGGSGG-NGGNAGMGGNSGTGSGDGGAGGNGGAAGTGGTGGDGGLTGT 758 +GG G G G + SG NGG G+G G G G + N G G+G G G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWG--GG 59 Query: 759 GGTGGSGGTGGDGGNGGNGADNTANMTAQAGGDGGNGGDGGFGGGAGAGGGGLTA 813 G G GG G GG G G + +A A G G G G L+A Sbjct: 60 SGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSA 114 Score = 31.6 bits (71), Expect = 0.022 Identities = 25/84 (29%), Positives = 30/84 (35%) Query: 809 GGLTAGANGTGGQGGAGGDGGNGAIGGHGPLTDDPGGNGGTGGNGGTGGTGGAGIGSLGG 868 GG G N +GG +G G +D G + GG G+G G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 869 GTGGDGGNGGNGGTGGEGGEVGGA 892 G GG GN G G G A Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAA 86 Score = 30.8 bits (69), Expect = 0.031 Identities = 35/114 (30%), Positives = 45/114 (39%), Gaps = 1/114 (0%) Query: 19 NGGNGADNTTTAAAGTTGGAGGAGGAGGTGGTGGAAGTGTGGQQGNGGNGGNGGTGGKGG 78 +GG+G + T A + + GG G G GG +G + GG+G GG G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 79 TGGDGALAGSSGGAGGKGGNGGDAGKAGTG-SAPGTAGTGGDGGKGGNGGIGAA 131 G G S GG+G G A G A T G GG G + AA Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAA 115 Score = 30.8 bits (69), Expect = 0.034 Identities = 27/81 (33%), Positives = 32/81 (39%) Query: 898 AAGNGGDGGTGGTGGGDGGAGGTGGTGGTGGLGDPRVGGSGGDGGTGGSGGAAGNGGNGG 957 + G+G TG GG G G GG D S + GGSG GG G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 958 NAGAGGNGNGGTGGAGGIGGT 978 + GGNGN G G G + Sbjct: 62 HGNGGGNGNSGGGSGTGGNLS 82 Score = 30.8 bits (69), Expect = 0.034 Identities = 28/102 (27%), Positives = 35/102 (34%) Query: 567 GDGGAGGTGGDRTVGGGTVPAGSGGQGGNAGGGGAGGQGGADGGSGGDGGDAGTGGNGGN 626 G G G G + G +G G G+G + GG G GG G+ Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 627 GGNRNSGNGTGGAGGNGGGGANGGAGGAGGSGGGTGGNGGAG 668 G +GN GG+G G A G T G GG Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 30.8 bits (69), Expect = 0.034 Identities = 32/100 (32%), Positives = 35/100 (35%) Query: 485 GAGGTGGAAGDGGQGGQGGAGGGAGGQGGAGGAGGTGGNGGNITGGTAGTAGAAGNGGAA 544 G G G G G G G GG G + N GG +G+ G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 545 GKGGAGGQGGTGGGTGGQGGAGGDGGAGGTGGDRTVGGGT 584 G GG G G G GTGG A A G T G G Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG 102 Score = 30.5 bits (68), Expect = 0.040 Identities = 30/85 (35%), Positives = 38/85 (44%), Gaps = 2/85 (2%) Query: 305 GIGGKGGNAGAGGAAGSNGGTVGANGTGGDGGNGGAAGAATAGSNGGAGTGSAGGNGGTG 364 G G+G N GA +G+ G G G G GG +G ++ + G G+GS GG Sbjct: 3 GGDGRGHNTGAHSTSGNING--GPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGS 60 Query: 365 GRGGSGGAGGDGIGGVGGGKGGNGA 389 G G GG G G G GG A Sbjct: 61 GHGNGGGNGNSGGGSGTGGNLSAVA 85 Score = 30.5 bits (68), Expect = 0.043 Identities = 29/80 (36%), Positives = 35/80 (43%), Gaps = 2/80 (2%) Query: 801 GGGAGAGGGGLTAGANGTGGQGGAGGDGGNGAIGGHGPLTDDPGGNGGTGGNGGTGGTGG 860 G G G G + N GG G G GG A G G +++ GG+G GG G Sbjct: 4 GDGRGHNTGAHSTSGNINGGPTGLGVGGG--ASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 861 AGIGSLGGGTGGDGGNGGNG 880 G G G +GG G GGN Sbjct: 62 HGNGGGNGNSGGGSGTGGNL 81 Score = 30.5 bits (68), Expect = 0.044 Identities = 27/85 (31%), Positives = 35/85 (41%) Query: 148 SGGAGGTGGDGGAANGGTAGAGGAGGNGGKGGDGGAGVTSSTAGNSGGAGGSGGKGGDAG 207 SGG G G + G G G G G G+G +S GG+G GG +G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 208 AGGAGATPGANGIAGNGGDGGDGAA 232 G G + G +G GG+ AA Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAA 86 Score = 30.5 bits (68), Expect = 0.048 Identities = 30/102 (29%), Positives = 39/102 (38%) Query: 368 GSGGAGGDGIGGVGGGKGGNGADGEVGGAGGAGGSGPNTSPGGNGGQGGQGGSGGAGGAA 427 G G G + G G G G G + GSG ++ GG G G G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 428 GAGGAGGGANGTAGNGGQGGAGGTGGAGAASSATNGGSGGAG 469 G GG G + G +G GG A A + + G+GG Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 30.5 bits (68), Expect = 0.048 Identities = 38/109 (34%), Positives = 43/109 (39%), Gaps = 11/109 (10%) Query: 961 AGGNGNGGTGGAGGIGGTGGNGGDAEPGVPPGAGGAGGAGTTGGKGGTGGNGSGTGSGGT 1020 +GG+G G GA G G P G GG + G + N G GSG Sbjct: 2 SGGDGRGHNTGAHSTSGNINGG--------PTGLGVGGGASDGSGWSSENNPWGGGSGSG 53 Query: 1021 GGDGGTGGGGGNGGTGWNGGKGDTGSGGGAGDGGKA---PAGGTGGAGG 1066 GG G G GG G +GG TG A A PA T GAGG Sbjct: 54 IHWGGGSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG 102
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv3514	cloacin	37	7e-04	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 37.0 bits (85), Expect = 7e-04 Identities = 33/102 (32%), Positives = 41/102 (40%) Query: 479 GAAGTGGTGGMIGTTGNAGVGGAGGQGGDGGAGGAGADADQPGATGGTGFAGGAGGAGGA 538 G G G G T+GN G G G G + G+G ++ GG+G GG G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 539 GGSSGAGGTNGSGGAGGTGGQGGAGGAGGAGADNPTGIGGTG 580 G G G + G G GG A A G A + G GG Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 36.6 bits (84), Expect = 9e-04 Identities = 34/109 (31%), Positives = 40/109 (36%) Query: 505 GGDGGAGGAGADADQPGATGGTGFAGGAGGAGGAGGSSGAGGTNGSGGAGGTGGQGGAGG 564 GGDG GA + GG G GGA G S G G G GG+G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 565 AGGAGADNPTGIGGTGGDGGTGGAAGAGGAGGAAGTGGTGGMIGTTGNA 613 G G N G GTGG+ A A G + G G + + A Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGA 111 Score = 36.2 bits (83), Expect = 0.001 Identities = 29/89 (32%), Positives = 35/89 (39%) Query: 1045 GAGGKGGAGGSSGAGGTNGSGGAGGAGGQGGAGGAGISFSNGSNGGTGGTGGVGGTGGDG 1104 G G+G G+ G G G G G + G+G S N GG G+G G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 1105 GNAGTGAGDPGKGGTGGTGGTGGSGGAGG 1133 GN G G GTGG + A G Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFG 91 Score = 36.2 bits (83), Expect = 0.001 Identities = 30/90 (33%), Positives = 38/90 (42%) Query: 1244 AGGPGGKGGAGGNAGTGGTNGSGAGGAGGQGGAGGAGISFSNGSNGGTGGTGGVGGTGGD 1303 +GG G G ++ +G NG G G G + G+G S N GG G+G G G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 1304 GGNAGTGAGDPGKGGTGGTGGTGGSGGAGG 1333 GN G G GTGG + A G Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAAPVAFG 91 Score = 35.8 bits (82), Expect = 0.001 Identities = 34/109 (31%), Positives = 40/109 (36%) Query: 622 GGDGGAGGAGADADQPGATGGTGFAGGAGGAGKAGGSSSAGGTNSSGSAGGTGRQSGTGG 681 GGDG GA + GG G GGA G SS GS G G+G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 682 AGGAGADNPTGIGGTGGDGGTGGAAGAGGAGGAAGTGGTGGMIGTTGNA 730 G G N G GTGG+ A A G + G G + + A Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGA 111 Score = 35.8 bits (82), Expect = 0.001 Identities = 27/89 (30%), Positives = 34/89 (38%) Query: 1378 GKGGTNGNGGSGGTGGTGGPGGSGGAPTGSGTGGKGGAGGDGGDGADGGAATGVGDGGDG 1437 G G N G+ T G G +G G + G G + + G G+ G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 1438 GNGGNGGNGGTGVGSPGGLGGAGGTGGLG 1466 GNGG GN G G G+ G L G Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFG 91 Score = 34.7 bits (79), Expect = 0.003 Identities = 36/101 (35%), Positives = 39/101 (38%), Gaps = 3/101 (2%) Query: 1321 GTGGTGGSGGAGGSGGANFNGGTGGTGGTGGTGGKGGMGGIAG--DGGPGGDGGNAGVGG 1378 G G G + GA + G N NGG G G GG G GG G G G Sbjct: 3 GGDGRGHNTGAHSTSG-NINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 1379 KGGTNGNGGSGGTGGTGGPGGSGGAPTGSGTGGKGGAGGDG 1419 G GNG SGG GTGG + AP G G G Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG 102 Score = 34.3 bits (78), Expect = 0.004 Identities = 31/97 (31%), Positives = 37/97 (38%) Query: 126 GGAGGAGGLLFGNGGAGAAGAPGQAGGPGGPAGLWGNGGPGGAGGSGGGTGGAGGAGGWL 185 GG G G + G+G + GG G WG G G GG G +GG G GG L Sbjct: 22 GGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNGNSGGGSGTGGNL 81 Query: 186 FGVGGAGGVGGAGGGTGGAGGPGGLIWGGGGAGGVGG 222 V G T GAGG I G + + Sbjct: 82 SAVAAPVAFGFPALSTPGAGGLAVSISAGALSAAIAD 118 Score = 34.3 bits (78), Expect = 0.004 Identities = 26/79 (32%), Positives = 34/79 (43%) Query: 1218 IGGDGGQGGNGGQGDSGSGLGGQPGFAGGPGGKGGAGGNAGTGGTNGSGAGGAGGQGGAG 1277 + G G+G N G + + G P G GG G + G G+G GG Sbjct: 1 MSGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGS 60 Query: 1278 GAGISFSNGSNGGTGGTGG 1296 G G NG++GG GTGG Sbjct: 61 GHGNGGGNGNSGGGSGTGG 79 Score = 33.9 bits (77), Expect = 0.005 Identities = 32/102 (31%), Positives = 40/102 (39%), Gaps = 1/102 (0%) Query: 1070 AGGQGGAGGAGISFSNGSNGGTGGTGGVGGTGGDGGNAGTGAGDPGKGGTGGTGGTGGSG 1129 +GG G G ++G+ G GVGG DG + +P GG+G GG Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSS-ENNPWGGGSGSGIHWGGGS 60 Query: 1130 GAGGSGGANFNGGTGGTGGTGGTGGKGGMGGIAGDGGPGGDG 1171 G G GG +GG GTGG G PG G Sbjct: 61 GHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG 102 Score = 33.9 bits (77), Expect = 0.005 Identities = 32/102 (31%), Positives = 40/102 (39%), Gaps = 1/102 (0%) Query: 1270 AGGQGGAGGAGISFSNGSNGGTGGTGGVGGTGGDGGNAGTGAGDPGKGGTGGTGGTGGSG 1329 +GG G G ++G+ G GVGG DG + +P GG+G GG Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSS-ENNPWGGGSGSGIHWGGGS 60 Query: 1330 GAGGSGGANFNGGTGGTGGTGGTGGKGGMGGIAGDGGPGGDG 1371 G G GG +GG GTGG G PG G Sbjct: 61 GHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG 102 Score = 33.9 bits (77), Expect = 0.005 Identities = 32/103 (31%), Positives = 39/103 (37%), Gaps = 1/103 (0%) Query: 793 GAAGTGGTGGMIGTTGNAGVGGAGGQGGDGGAGGAGADADQPGATGGTGFAGGAGGAGGS 852 G G G G T+GN G G G G + G+G ++ GG+G GG G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 853 GGSSCAGGTNGSGGAGGTCGQVVAGGAGISFSNGSNGGTGGTG 895 G G + G G GG VA F S G GG Sbjct: 63 GNGGGNGNSGGGSGTGGN-LSAVAAPVAFGFPALSTPGAGGLA 104 Score = 33.9 bits (77), Expect = 0.005 Identities = 37/108 (34%), Positives = 42/108 (38%), Gaps = 5/108 (4%) Query: 1100 TGGDGGNAGTGAGDPGKGGTGGTGGTGGSGGAGGSGGANFNGGTGGTGGTGGTGGKGGMG 1159 +GGDG TGA GG G G GGA G + G G G GG G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 1160 GIAGDGGPGGDGGNAGVGGKGGTNGNGGSGGTGGTGGAGGNAGAGGLA 1207 G GG GN+G G G N + + A GAGGLA Sbjct: 62 -----HGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 33.5 bits (76), Expect = 0.007 Identities = 27/89 (30%), Positives = 32/89 (35%) Query: 1369 GDGGNAGVGGKGGTNGNGGSGGTGGTGGPGGSGGAPTGSGTGGKGGAGGDGGDGADGGAA 1428 G G G T+GN G TG G G S G+ S GG G G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 1429 TGVGDGGDGGNGGNGGNGGTGVGSPGGLG 1457 G G+ G G G + V +P G Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFG 91 Score = 32.8 bits (74), Expect = 0.011 Identities = 28/102 (27%), Positives = 36/102 (35%) Query: 596 GAAGTGGTGGMIGTTGNAGVGGAGGQGGDGGAGGAGADADQPGATGGTGFAGGAGGAGKA 655 G G G G T+GN G G G G + G+G ++ GG+G GG Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 656 GGSSSAGGTNSSGSAGGTGRQSGTGGAGGAGADNPTGIGGTG 697 G G + GG A G A + G GG Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 32.8 bits (74), Expect = 0.012 Identities = 36/119 (30%), Positives = 45/119 (37%), Gaps = 10/119 (8%) Query: 713 GAAGTGGTGGMIGTTGNAGVGGAGGSSGAGGTNGSGGAGGTDGQGGAGGAGGAGADNPTG 772 G G G G T+GN G G G G ++GSG + + GG G+G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSG--------- 53 Query: 773 IGGTGGDGGTGGAAGAGGAGGAAGTGGTGGMIGTTGNAGVGGAGGQGGDGGAGGAGADA 831 GG G G G G +GG +GTGG + G G G A A A Sbjct: 54 -IHWGGGSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGA 111 Score = 32.8 bits (74), Expect = 0.014 Identities = 34/101 (33%), Positives = 37/101 (36%), Gaps = 2/101 (1%) Query: 391 GGDGGAGGAGADADQPGATGGTGFAGGAGGAGGAGGSSGAGGTNGSGGAGGQGGAGGAGG 450 GGDG GA + GG G GGA G S G G G GG+G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 451 AGADNPTGIGGTGGDGGTGGAAGAGGAGG--AAGTGGTGGM 489 GG G GG A A A G A T G GG+ Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGL 103 Score = 32.4 bits (73), Expect = 0.014 Identities = 33/112 (29%), Positives = 37/112 (33%), Gaps = 9/112 (8%) Query: 1183 NGNGGSGGTGGTGGAGGNAGAGGLANTGGTAGNAGIGGDGGQGGNGGQGDSGSGLGGQPG 1242 +G G G G GN G G + G G GG SG GG G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 1243 FAGGPGGKGGAGGNAGTGGTNGSGAGGAGGQG-------GAGGAGISFSNGS 1287 G GG G G G N S G GAGG +S S G+ Sbjct: 62 --HGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGA 111 Score = 32.4 bits (73), Expect = 0.015 Identities = 40/128 (31%), Positives = 47/128 (36%), Gaps = 23/128 (17%) Query: 965 SGTGGTGGTGGKGGTGGAGDDSAGGTGGTGGAGGNAGAGGLANTGGTAGNAGIGGDGGQG 1024 SG G G G T G + G G GGA +G N G +GI GG G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 1025 GNGGQGDSGSGLGGQPGFAGGAGGKGGAGGSSGAGGTNGSGGAGGAGG-----QGGAGGA 1079 G GG G +GG SG GG + A A G GAGG Sbjct: 62 H------------------GNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGL 103 Query: 1080 GISFSNGS 1087 +S S G+ Sbjct: 104 AVSISAGA 111 Score = 32.0 bits (72), Expect = 0.023 Identities = 30/101 (29%), Positives = 39/101 (38%) Query: 363 GGAGGAAGQLFSASGAAGNAGVGGAGGQGGDGGAGGAGADADQPGATGGTGFAGGAGGAG 422 G G S SG G G G G+G + + G +G GG G G Sbjct: 4 GDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHG 63 Query: 423 GAGGSSGAGGTNGSGGAGGQGGAGGAGGAGADNPTGIGGTG 463 GG+ +GG +G+GG A A G A + G GG Sbjct: 64 NGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 31.2 bits (70), Expect = 0.040 Identities = 25/74 (33%), Positives = 31/74 (41%) Query: 877 GGAGISFSNGSNGGTGGTGGVGGTGGDGGNAGTGAGDPGKGGTGGTGGTGGSGGAGGSGG 936 G + S N G TG G G G+ + +P GG+G GG G G GG Sbjct: 8 GHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGG 67 Query: 937 ANFNGGTGGTGGTG 950 +GG GTGG Sbjct: 68 NGNSGGGSGTGGNL 81 Score = 30.8 bits (69), Expect = 0.045 Identities = 26/82 (31%), Positives = 36/82 (43%) Query: 856 SCAGGTNGSGGAGGTCGQVVAGGAGISFSNGSNGGTGGTGGVGGTGGDGGNAGTGAGDPG 915 S G + GA T G + G G+ G++ G+G + GG G+ G G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 916 KGGTGGTGGTGGSGGAGGSGGA 937 G GG G +GG G GG+ A Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSA 83
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv3517	cdtoxina	30	0.007	Cytolethal distending toxin A signature.
>cdtoxina#Cytolethal distending toxin A signature. Length = 258 Score = 30.1 bits (67), Expect = 0.007 Identities = 8/17 (47%), Positives = 11/17 (64%) Query: 37 GADLTAWSRAQAAWLWS 53 G+ LT WSR + LW+ Sbjct: 87 GSVLTMWSRGAGSSLWA 103

Rv3590c

Rv3600c

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
Rv3590c	5	10	5.223410	PE-PGRS family protein PE_PGRS58
Rv3591c	0	10	1.525190	Possible hydrolase
Rv3592	0	12	1.410262	Possible heme degrading protein MhuD
Rv3593	1	10	0.238616	Probable conserved lipoprotein LpqF
Rv3594	2	11	0.468136	Conserved hypothetical protein
Rv3595c	2	10	0.015033	PE-PGRS family protein PE_PGRS59
Rv3596c	3	11	-2.460790	Probable ATP-dependent protease ATP-binding
Rv3597c	1	12	-2.369163	Iron-regulated H-NS-like protein Lsr2
Rv3598c	0	12	-1.344507	Lysyl-tRNA synthetase 1 LysS (lysine--tRNA
Rv3599c	-1	13	1.710105	Hypothetical short protein
Rv3600c	-2	13	1.842755	Conserved protein

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv3590c	cloacin	39	3e-05	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 39.3 bits (91), Expect = 3e-05 Identities = 43/132 (32%), Positives = 50/132 (37%), Gaps = 2/132 (1%) Query: 449 AGTGGVGGVGGAGGQGGLLFGDGGNGGAGGAGGIGGTGASGGAGGKGGSGLVGGDGGNGG 508 +G G G GA G + G G GG G +S GGSG GG G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 509 AGGAGGNGGKGGAGGAGGGAGMFSQPGVHG--AGGTGGQGGAGGAGGAGGAAGAGTVVAG 566 G GGNG GG G GG + P G A T G GG + AG + A + Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSAAIADIMA 121 Query: 567 NPGDPGGFGAAG 578 P FG G Sbjct: 122 ALKGPFKFGLWG 133 Score = 38.2 bits (88), Expect = 1e-04 Identities = 32/91 (35%), Positives = 41/91 (45%) Query: 136 GGPGGLLWGNGGNGGSGVAGVGGPGGSGGAAGLFGHGGNGGAGGSNAAGAGGVGGAGGAG 195 GGP GL G G + GSG + P G G +G+ GG+G G +GG G GG Sbjct: 22 GGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNGNSGGGSGTGGNL 81 Query: 196 WLVGNGGAGGFGGVGTTVSGNGGAGGAAGAF 226 V A GF + T +G +AGA Sbjct: 82 SAVAAPVAFGFPALSTPGAGGLAVSISAGAL 112 Score = 35.5 bits (81), Expect = 5e-04 Identities = 31/84 (36%), Positives = 40/84 (47%), Gaps = 5/84 (5%) Query: 314 AGGAGGLGGSGSDTSEGPVTGGNGGNGGDGGPGAPGGNGA---PGGIGVNTGTGWAYGGN 370 +GG G +G+ ++ G + GG G G GG G + P G G +G W GG Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHW--GGG 59 Query: 371 GGNGGDGGAGARGGDGGNGGNGLA 394 G+G GG G GG G GGN A Sbjct: 60 SGHGNGGGNGNSGGGSGTGGNLSA 83 Score = 33.5 bits (76), Expect = 0.003 Identities = 30/97 (30%), Positives = 36/97 (37%), Gaps = 10/97 (10%) Query: 395 LNGGNGIGGNGGAGGRGGTGAAGGNGGIGGGATGTLTFFGSGGDGGPGGAGANTAGTGGV 454 ++GG+G G N GA G GG G+G G G G G + N GG Sbjct: 1 MSGGDGRGHNTGAHSTSGN-INGGPTGLGV---------GGGASDGSGWSSENNPWGGGS 50 Query: 455 GGVGGAGGQGGLLFGDGGNGGAGGAGGIGGTGASGGA 491 G GG G G G GG+G G A Sbjct: 51 GSGIHWGGGSGHGNGGGNGNSGGGSGTGGNLSAVAAP 87 Score = 32.8 bits (74), Expect = 0.004 Identities = 33/97 (34%), Positives = 37/97 (38%), Gaps = 3/97 (3%) Query: 145 NGGNGGSGVAGVGGPGGSGGAAGLFGHGGNGGAGGSNAAGAGGVGGAGGAGWLVGNGGAG 204 N G + GGP G G G G G+G GG GNGG Sbjct: 10 NTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG-HGNGGGN 68 Query: 205 GFGGVGTTVSGNGGAGGAAGAFGNGGVG--GAGGAAV 239 G G G+ GN A A AFG + GAGG AV Sbjct: 69 GNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAV 105 Score = 32.8 bits (74), Expect = 0.004 Identities = 28/81 (34%), Positives = 32/81 (39%), Gaps = 3/81 (3%) Query: 383 GGDGGNGGNGLALNGGNGIGGNGGAGGRGGTGAAGGNGGIG---GGATGTLTFFGSGGDG 439 GGDG G GN GG G G GG G GG +G+ +G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 440 GPGGAGANTAGTGGVGGVGGA 460 G GG N+ G G GG A Sbjct: 63 GNGGGNGNSGGGSGTGGNLSA 83 Score = 32.8 bits (74), Expect = 0.004 Identities = 25/76 (32%), Positives = 30/76 (39%) Query: 291 GANNGAGSGGAGLPGNPGAVPGRAGGAGGLGGSGSDTSEGPVTGGNGGNGGDGGPGAPGG 350 G N GA S + G P + G + G G S + G +G GG G G GG Sbjct: 8 GHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGG 67 Query: 351 NGAPGGIGVNTGTGWA 366 NG GG G A Sbjct: 68 NGNSGGGSGTGGNLSA 83 Score = 32.4 bits (73), Expect = 0.005 Identities = 32/128 (25%), Positives = 44/128 (34%), Gaps = 10/128 (7%) Query: 335 GNGGNGGDGGPGAPGGN--GAPGGIGVNTGTGWAYGGNGGNGGDGGAGARGGDGGNGGNG 392 G G G + G + GN G P G+GV GG G G G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVG-------GGASDGSGWSSENNPWGGGSGSGIH 55 Query: 393 LALNGGNGIGGNGGAGGRGGTGAAGGNGGIGGGATGTLTFFGSGGDGGPGGAGANTAGTG 452 G+G GG G G GG+G G + + G GG + + A + Sbjct: 56 WGGGSGHGNGGGNGNSG-GGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSA 114 Query: 453 GVGGVGGA 460 + + A Sbjct: 115 AIADIMAA 122 Score = 31.6 bits (71), Expect = 0.009 Identities = 29/102 (28%), Positives = 36/102 (35%) Query: 174 NGGAGGSNAAGAGGVGGAGGAGWLVGNGGAGGFGGVGTTVSGNGGAGGAAGAFGNGGVGG 233 +GG G + GA G G G G G G + N GG+ GG G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 234 AGGAAVIGGLPGNGGAGGNAGLIGAGGDGGVGGVGAPGTNGM 275 G G G G GGN + A G + PG G+ Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGL 103 Score = 31.6 bits (71), Expect = 0.010 Identities = 31/102 (30%), Positives = 37/102 (36%), Gaps = 2/102 (1%) Query: 483 GGTGASGGAGGKGGSGLVGGDGGNGGAGGAGGNGGKGGAGGAGGGAGMFSQPGVHGAGGT 542 GG G G SG + +GG G G GG G G S G+H GG+ Sbjct: 3 GGDGRGHNTGAHSTSGNI--NGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGS 60 Query: 543 GGQGGAGGAGGAGGAAGAGTVVAGNPGDPGGFGAAGADGLPG 584 G G G GG+ G + A GF A G G Sbjct: 61 GHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG 102 Score = 30.8 bits (69), Expect = 0.016 Identities = 37/108 (34%), Positives = 41/108 (37%), Gaps = 8/108 (7%) Query: 160 GGSGGAAGLFGHGGNGGAGGSNAAGAGGVGGAGGAGWLVGNGGAGGFGGVGTTVSGN--- 216 GG G H +G G G G + G+GW N GG G G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 217 ---GGAGGAAGAFGNGGVGGAGGAAVIGGLPG--NGGAGGNAGLIGAG 259 GG G + G G GG A A V G P GAGG A I AG Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAG 110
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv3593	BLACTAMASEA	39	3e-05	Beta-lactamase class A signature.
>BLACTAMASEA#Beta-lactamase class A signature. Length = 286 Score = 39.0 bits (91), Expect = 3e-05 Identities = 24/137 (17%), Positives = 46/137 (33%), Gaps = 3/137 (2%) Query: 148 PSIASWRDVDAALSKTGARYSFQVAKVDNGRCDPVAGTNTGESLPLASIFKLYVLHALAG 207 S + + S+ R + ++D + E P+ S FK+ + A+ Sbjct: 21 ASPQPLEQIKLSESQLSGR--VGMIEMDLASGRTLTAWRADERFPMMSTFKVVLCGAVLA 78 Query: 208 AVQHNTVSWDDLLTVTAKSKAVGSSGLELPVGARVSVRTAAEKMIATSDNMATDLLIERL 267 V + + + S E + ++V I SDN A +LL+ + Sbjct: 79 RVDAGDEQLERKIHYRQQDLVDYSPVSEKHLADGMTVGELCAAAITMSDNSAANLLLATV 138 Query: 268 -GTRAIEEALASAGHHD 283 G + L G + Sbjct: 139 GGPAGLTAFLRQIGDNV 155
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv3595c	cloacin	39	4e-05	Cloacin signature.
>cloacin#Cloacin signature. Length = 551 Score = 38.5 bits (89), Expect = 4e-05 Identities = 31/95 (32%), Positives = 36/95 (37%) Query: 152 GGSGGAAGLIGNGGSGGAGGAGAAGGSGGQGGLLYGNGGAGGNGGAATIPGGNGGAGGAG 211 G + GA GN G G G S G G N GG+G GG+G G G Sbjct: 8 GHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGG 67 Query: 212 GNAWLFGNGGAGGLGAAGAAGAAGVNPLTVPAGQG 246 G+G G L A A A G L+ P G Sbjct: 68 NGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG 102 Score = 37.4 bits (86), Expect = 1e-04 Identities = 34/108 (31%), Positives = 40/108 (37%), Gaps = 8/108 (7%) Query: 309 GGAGGIGGTGGEGGIGARGGTGGQGGMGGAGQPGVGGDAGDGGNGGIGGDGGAGGDGGAG 368 GG G TG G G G+GG G G + + GG G G G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 369 GAGGAGGLFGVSGSSGLGGAAGSGGNGGGGGEPGVAGSPGVGPAGRGG 416 G GG G GG +G+GGN P G P + G GG Sbjct: 63 GNGGGNG--------NSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG 102 Score = 35.1 bits (80), Expect = 6e-04 Identities = 33/93 (35%), Positives = 37/93 (39%), Gaps = 3/93 (3%) Query: 119 GADGTAPGQNGGAGGLLYGNGGNGAAG---VNAGIAGGSGGAAGLIGNGGSGGAGGAGAA 175 GA T+ NGG GL G G + +G N GGSG G G G GG G + Sbjct: 12 GAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGHGNGGGNGNS 71 Query: 176 GGSGGQGGLLYGNGGAGGNGGAATIPGGNGGAG 208 GG G GG L G A G GG Sbjct: 72 GGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA 104 Score = 34.7 bits (79), Expect = 7e-04 Identities = 35/106 (33%), Positives = 41/106 (38%), Gaps = 1/106 (0%) Query: 286 TGGTGGTGGAGGSGGRGGLLVGDGGAGGIGGTGGEGGIGARGGTGGQGGMGGAGQPGVGG 345 +GG G G G + G G G GG G + G G G G G Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 346 DAGDGGNGGIGGDGGAGGDGGAGGAGGAGGLFGVSGSSGLGGAAGS 391 GGNG GG G GG+ A A A G +S + G GG A S Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALS-TPGAGGLAVS 106 Score = 31.2 bits (70), Expect = 0.010 Identities = 33/106 (31%), Positives = 39/106 (36%), Gaps = 6/106 (5%) Query: 130 GAGGLLYGNGGNGAAGVNAGIAGGSGGAAGLIGNGGSGGAGGAGAAGGSGGQGGLLYGNG 189 G G + G + +G G G G G + GSG + GG G G G Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGG--ASDGSGWSSENNPWGGGSGSGIHWGGGS 60 Query: 190 GAGGNGGAATIPGGNGGAGGAGGNAWLFGNGGAGGLGAAGAAGAAG 235 G G GG G +GG G GGN A G A GA G Sbjct: 61 GHGNGGGN----GNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG 102 Score = 30.5 bits (68), Expect = 0.013 Identities = 27/82 (32%), Positives = 29/82 (35%), Gaps = 1/82 (1%) Query: 267 TGGTG-GTGGTGLSVGGTGGTGGTGGTGGAGGSGGRGGLLVGDGGAGGIGGTGGEGGIGA 325 +GG G G S G G TG G G S G G + GG G GG Sbjct: 2 SGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSG 61 Query: 326 RGGTGGQGGMGGAGQPGVGGDA 347 G GG G GG G A Sbjct: 62 HGNGGGNGNSGGGSGTGGNLSA 83 Score = 29.3 bits (65), Expect = 0.034 Identities = 29/91 (31%), Positives = 36/91 (39%) Query: 342 GVGGDAGDGGNGGIGGDGGAGGDGGAGGAGGAGGLFGVSGSSGLGGAAGSGGNGGGGGEP 401 G G + G G+ G G G G + G S ++ GG +GSG + GGG Sbjct: 3 GGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNPWGGGSGSGIHWGGGSGH 62 Query: 402 GVAGSPGVGPAGRGGDGNLGQFGPEGAPGQP 432 G G G G G GNL A G P Sbjct: 63 GNGGGNGNSGGGSGTGGNLSAVAAPVAFGFP 93
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv3596c	HTHFIS	32	0.007	FIS bacterial regulatory protein HTH signature.
>HTHFIS#FIS bacterial regulatory protein HTH signature. Length = 484 Score = 32.5 bits (74), Expect = 0.007 Identities = 31/160 (19%), Positives = 59/160 (36%), Gaps = 28/160 (17%) Query: 518 IIGQEDAVKAVSKAIRRTRAGLKDPKRPSGSFIFAGPSGVGKTELSKALANFLFGDDDAL 577 ++G+ A++ + + + R + + + G SG GK +++AL ++ + Sbjct: 139 LVGRSAAMQEIYRVLARL-------MQTDLTLMITGESGTGKELVARALHDYGKRRNGPF 191 Query: 578 IQIDMGEFHDRFTASRLFGAPPGYVGYEEGGQLTEKVRRKP--FS-----VVLFDEIEKA 630 + I+M S LFG E G T R F + DEI Sbjct: 192 VAINMAAIPRDLIESELFGH--------EKGAFTGAQTRSTGRFEQAEGGTLFLDEIGDM 243 Query: 631 HQEIYNSLLQVLEDG---RLTDGQGRTVDFKNTVLIFTSN 667 + LL+VL+ G + D + ++ +N Sbjct: 244 PMDAQTRLLRVLQQGEYTTVGGRTPIRSDVR---IVAATN 280
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv3600c	PF03309	361	e-129	Bvg accessory factor
>PF03309#Bvg accessory factor Length = 271 Score = 361 bits (927), Expect = e-129 Identities = 234/272 (86%), Positives = 252/272 (92%), Gaps = 1/272 (0%) Query: 1 MLLAIDVRNTHTVVGLLSGMKEHAKVVQQWRIRTESEVTADELALTIDGLIGEDSERLTG 60 MLLAIDVRNTHTVVGL+SG +HAKVVQQWRIRTE EVTADELALTIDGLIG+D+ERLTG Sbjct: 1 MLLAIDVRNTHTVVGLISGSGDHAKVVQQWRIRTEPEVTADELALTIDGLIGDDAERLTG 60 Query: 61 TAALSTVPSVLHEVRIMLDQYWPSVPHVLIEPGVRTGIPLLVDNPKEVGADRIVNCLAAY 120 + LSTVPSVLHEVR+ML+QYWP+VPHVLIEPGVRTGIPLLVDNPKEVGADRIVNCLAAY Sbjct: 61 ASGLSTVPSVLHEVRVMLEQYWPNVPHVLIEPGVRTGIPLLVDNPKEVGADRIVNCLAAY 120 Query: 121 DRFRKAAIVVDFGSSICVDVVSAKGEFLGGAIAPGVQVSSDAAAARSAALRRVELARPRS 180 ++ AAIVVDFGSSICVDVVSAKGEFLGGAIAPGVQVSSDAAAARSAALRRVEL RPRS Sbjct: 121 HKYGTAAIVVDFGSSICVDVVSAKGEFLGGAIAPGVQVSSDAAAARSAALRRVELTRPRS 180 Query: 181 VVGKNTVECMQAGAVFGFAGLVDGLVGRIREDVSGFSVDHDVAIVATGHTAPLLLPELHT 240 V+GKNTVECMQAGAVFGFAGLVDGLV RIR+DV GFS DVA+VATGHTAPL+LP+L T Sbjct: 181 VIGKNTVECMQAGAVFGFAGLVDGLVNRIRDDVDGFS-GADVAVVATGHTAPLVLPDLRT 239 Query: 241 VDHYDQHLTLQGLRLVFERNLEVQRGRLKTAR 272 V+HYD+HLTL GLRLVFERN QRG+LK AR Sbjct: 240 VEHYDRHLTLDGLRLVFERNRANQRGKLKPAR 271

Rv3876

Rv3886c

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
Rv3876	0	18	0.155139	ESX-1 secretion-associated protein EspI.
Rv3877	0	16	0.132249	ESX conserved component EccD1. ESX-1 type VII
Rv3878	0	13	0.929651	ESX-1 secretion-associated protein EspJ.
Rv3879c	0	14	0.029304	ESX-1 secretion-associated protein EspK. Alanine
Rv3880c	-2	13	-0.427705	ESX-1 secretion-associated protein EspL
Rv3881c	0	14	0.055237	Secreted ESX-1 substrate protein B, EspB.
Rv3882c	2	14	0.013157	ESX conserved component EccE1. ESX-1 type VII
Rv3883c	1	15	-0.704325	Membrane-anchored mycosin MycP1 (serine
Rv3884c	1	15	-1.086244	ESX conserved component EccA2. ESX-2 type VII
Rv3885c	3	17	-0.685389	ESX conserved component EccE2. ESX-2 type VII
Rv3886c	4	17	-1.105920	Probable alanine and proline rich

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv3876	TONBPROTEIN	42	3e-06	Gram-negative bacterial tonB protein signature.
>TONBPROTEIN#Gram-negative bacterial tonB protein signature. Length = 239 Score = 42.3 bits (99), Expect = 3e-06 Identities = 29/126 (23%), Positives = 38/126 (30%), Gaps = 5/126 (3%) Query: 71 PPPPPPPTPMPIAAGEPPSPEPAASKPPTPPMPIAGPEPAPPKPPTPPMPIAGPEPAPPK 130 P P P + + + P+ P P PEP P P P+ I P+P P Sbjct: 39 PAPAQPISVTMVTPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKP 98 Query: 131 PPTP-PMPIAGPAPTPTESQLAPPRPPTPQTPTGAPQQPESPAPHVP----SHGPHQPRR 185 P P P + P P P + A P + GP R Sbjct: 99 KPKPVKKVQEQPKRDVKPVESRPASPFENTAPARLTSSTATAATSKPVTSVASGPRALSR 158 Query: 186 TAPAPP 191 P P Sbjct: 159 NQPQYP 164 Score = 40.0 bits (93), Expect = 1e-05 Identities = 28/137 (20%), Positives = 38/137 (27%), Gaps = 6/137 (4%) Query: 92 PAASKPPTPPMPIAGPEPAPPKPPTPPMPIAGPEPAPPKPPTPPMPIAGPAPTPTESQLA 151 PA ++P + M P PP P+ PEP P P PP P Sbjct: 39 PAPAQPISVTMVTPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKP 98 Query: 152 PPRPPTPQTPTGAPQQPESPAPHVPSHGPHQPRRTAPAPPWAKMPIGEPPPAPSRPSASP 211 P+P + P R + P AS Sbjct: 99 KPKPVKKVQEQPKRDVKPVESRPASPFENTAPARLTSSTA------TAATSKPVTSVASG 152 Query: 212 AEPPTRPAPQHSRRARR 228 +R PQ+ RA+ Sbjct: 153 PRALSRNQPQYPARAQA 169 Score = 36.1 bits (83), Expect = 2e-04 Identities = 25/116 (21%), Positives = 35/116 (30%), Gaps = 5/116 (4%) Query: 34 PPAPASANLPKPNGQTPPPTSDDLSERFVSAPPPPPPPPPPPPPTPMPIAAGEPPSPEPA 93 P P S + P PP E V P P P P PP P+ I +P Sbjct: 41 PAQPISVTMVTPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKP 100 Query: 94 ASKPPTPPMPIAGPEPAPPKPPTPPMPIAGPEPAPPKPPTPPMPIAGPAPTPTESQ 149 P +P +P +P AP + + A P + + Sbjct: 101 KPVKKVQEQPKRDVKPVESRPASPFE-----NTAPARLTSSTATAATSKPVTSVAS 151 Score = 30.7 bits (69), Expect = 0.014 Identities = 22/122 (18%), Positives = 33/122 (27%) Query: 109 PAPPKPPTPPMPIAGPEPAPPKPPTPPMPIAGPAPTPTESQLAPPRPPTPQTPTGAPQQP 168 PAP +P + M P PP P+ P P P P P +P Sbjct: 39 PAPAQPISVTMVTPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKP 98 Query: 169 ESPAPHVPSHGPHQPRRTAPAPPWAKMPIGEPPPAPSRPSASPAEPPTRPAPQHSRRARR 228 + P + + + P + P S + + P R R Sbjct: 99 KPKPVKKVQEQPKRDVKPVESRPASPFENTAPARLTSSTATAATSKPVTSVASGPRALSR 158 Query: 229 GH 230 Sbjct: 159 NQ 160
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv3879c	PF05616	45	1e-06	Neisseria meningitidis TspB protein
>PF05616#Neisseria meningitidis TspB protein Length = 501 Score = 44.7 bits (105), Expect = 1e-06 Identities = 47/174 (27%), Positives = 64/174 (36%), Gaps = 15/174 (8%) Query: 173 LEDLLQQKSPPPPDVPTLVVPSPGTPG-TPGTPITPGTPITPGTPITPIPGAPVTPITPT 231 LE++L K PD + + G PG + + PGT + G P+T G PV + Sbjct: 240 LEEILSLKVDANPDK---YIKATGYPGYSEKVEVAPGTKVNMG-PVTDRNGNPVQVVATF 295 Query: 232 PGTPVTPVTPGKPVTPVTPVKPGTPGEPTPITPVTPPVAPATPATPATPVTPAPAPHPQP 291 T V P + PG+ P P+ P V+PA PA P P Sbjct: 296 GRDSQGNTTVDVQVIPRPDLTPGSAEAPNA-QPL-PEVSPAE--------NPANNPAPNE 345 Query: 292 APAPAPSPGPQPVTPATPGPSGPATPGTPGGEPAPHVKPAALAEQPGVPGQHAG 345 P P+P P P P PGT PA +P + G+ G Sbjct: 346 NPGTRPNPEPDPDLNPDANPDTDGQPGTRPDSPAVPDRPNGRHRKERKEGEDGG 399
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv3881c	TONBPROTEIN	33	0.002	Gram-negative bacterial tonB protein signature.
>TONBPROTEIN#Gram-negative bacterial tonB protein signature. Length = 239 Score = 32.7 bits (74), Expect = 0.002 Identities = 18/86 (20%), Positives = 25/86 (29%), Gaps = 1/86 (1%) Query: 271 EPVNPPKPPPAIKIDPPPPPQEQGLIPGFLMPPSDGSGVTPGTGMPAAPMVPPTG-SPGG 329 P +P P + P PP + +I P V P P Sbjct: 64 PPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKPVKKVQEQPKRDVKPVESRPAS 123 Query: 330 GLPADTAAQLTSAGREAAALSGDVAV 355 A+LTS+ AA +V Sbjct: 124 PFENTAPARLTSSTATAATSKPVTSV 149
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv3883c	SUBTILISIN	176	1e-53	Subtilisin serine protease family (S8) signature.
>SUBTILISIN#Subtilisin serine protease family (S8) signature. Length = 326 Score = 176 bits (448), Expect = 1e-53 Identities = 86/343 (25%), Positives = 129/343 (37%), Gaps = 69/343 (20%) Query: 64 PWSNTYLGVADAHKFATGAGVTVAVIDTGVDAS-PRVP--AEPGGDFVDQAG---NGLSD 117 P + G GV VAV+DTG DA P + G +F D D Sbjct: 23 PRGVEMIQAPAVWNQTRGRGVKVAVLDTGCDADHPDLKARIIGGRNFTDDDEGDPEIFKD 82 Query: 118 CDAHGTLTASIIAGRPAPTDGFVGVAPDARLLSLRQTSEAFEPVGSQANPNDPNATPAAG 177 + HGT A IA +G VGVAP+A LL ++ GS G Sbjct: 83 YNGHGTHVAGTIAATE-NENGVVGVAPEADLLIIK----VLNKQGS-------------G 124 Query: 178 SIRSLARAVVHAANLGVGVINISEAACYKVSRPIDETSLGASIDYAVNVKGVVVVVAAGN 237 + + + +A V +I++S + P D L ++ AV ++V+ AAGN Sbjct: 125 QYDWIIQGIYYAIEQKVDIISMS------LGGPEDVPELHEAVKKAVA-SQILVMCAAGN 177 Query: 238 TGGDCVQNPAPDPSTPGDPRGWNNVQTVVTPAWYAPLVLSVGGIGQTGMPSSFSMHGPWV 297 G G + + P Y V+SVG I S FS V Sbjct: 178 EG-----------------DGDDRTDELGYPGCY-NEVISVGAINFDRHASEFSNSNNEV 219 Query: 298 DVAAPAENIVALGDTGEPVNALQGREGPVPIAGTSFAAAYVSGLAALLRQRFP-----DL 352 D+ AP E+I++ G+ +GTS A +V+G AL++Q DL Sbjct: 220 DLVAPGEDILSTVPGGKYAT----------FSGTSMATPHVAGALALIKQLANASFERDL 269 Query: 353 TPAQIIHRITATARHPGGGVDDLVGAGVIDAVAA----LTWDI 391 T ++ ++ P G + G G++ A +D Sbjct: 270 TEPELYAQLIKRTI-PLGNSPKMEGNGLLYLTAVEELSRIFDT 311
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv3885c	PF06580	29	0.048	Sensor histidine kinase
>PF06580#Sensor histidine kinase Length = 349 Score = 29.1 bits (65), Expect = 0.048 Identities = 10/57 (17%), Positives = 22/57 (38%) Query: 24 VLASAGWALGGQLGAVMAVVVGVALVFVQWWGQPAWSWAVLGLRGRRPVKWNDPITL 80 + GW ++ V+ ++ + W+ W +L +PV + P+ L Sbjct: 62 FIKRQGWLKLNMGQIILRVLPACVVIGMVWFVANTSIWRLLAFINTKPVAFTLPLAL 118
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
Rv3886c	SUBTILISIN	118	8e-32	Subtilisin serine protease family (S8) signature.
>SUBTILISIN#Subtilisin serine protease family (S8) signature. Length = 326 Score = 118 bits (298), Expect = 8e-32 Identities = 57/259 (22%), Positives = 99/259 (38%), Gaps = 63/259 (24%) Query: 236 GVVGVAPHATIISIRQSSRAFEPVNPSSAGPNSDEKVKAGTLDSVARAVVHAANMGAKVI 295 GVVGVAP A ++ I+ + K +G D + + + +A +I Sbjct: 102 GVVGVAPEADLLIIKVLN-----------------KQGSGQYDWIIQGIYYAIEQKVDII 144 Query: 296 NISVTACLPAAAPGDQRVLGAALWYAATVKDAVIVAAAGNDGEAGCGNNPMYDPLDPSDP 355 ++S+ P D L A+ A +++ AAGN+G+ + Sbjct: 145 SMSL------GGPEDVPELHEAVKKA-VASQILVMCAAGNEGDGDDRTDE---------- 187 Query: 356 RDWHQVTVVSSPSWFSDYVLSVGAVDAYGAALDKSMSGPWVGVAAPGTHIMGLSPQGGGP 415 + P + + V+SVGA++ A + S S V + APG I+ P G Sbjct: 188 --------LGYPGCY-NEVISVGAINFDRHASEFSNSNNEVDLVAPGEDILSTVPGG--- 235 Query: 416 VNAYPPSRPGEKNMPFWGTSFSAAYVSGVAALVRAKFP-----ELTAYQVINRIVQSAHN 470 K F GTS + +V+G AL++ +LT ++ ++++ Sbjct: 236 -----------KYATFSGTSMATPHVAGALALIKQLANASFERDLTEPELYAQLIKRT-I 283 Query: 471 PPAGVDNKLGYGLVDPVAA 489 P G GL+ A Sbjct: 284 PLGNSPKMEGNGLLYLTAV 302 Score = 64.1 bits (156), Expect = 3e-13 Identities = 24/85 (28%), Positives = 39/85 (45%), Gaps = 7/85 (8%) Query: 71 PDVAQLAPGFNLVNISKAWQYSTGNGVPVAVIDTGVSPN-PRLP--VVPGGDYIMGEDG- 126 V ++ G ++ W + G GV VAV+DTG + P L ++ G ++ ++G Sbjct: 17 QQVNEIPRGVEMIQAPAVWNQTRGRGVKVAVLDTGCDADHPDLKARIIGGRNFTDDDEGD 76 Query: 127 ---LSDCDAHGTVVSSIIAAAPLGI 148 D + HGT V+ IAA Sbjct: 77 PEIFKDYNGHGTHVAGTIAATENEN 101


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