:: PredictBias ::

A) Input parameters
Genome	ZS7.gb	Threshold dinucleotide bias	2
Threshold codon bias	4	Threshold %GC bias	3
E-value (RPSBlast)	0.05	Genome (non-pathogenic)

B) Compare a potential GI or PAI in related non-pathogenic sp. (phylogenetic tree)
Potential GI or PAI start end
Select Organism

C) Potential GIs and PAIs in NC_011728 (download)

S.No

Start

End

Bias

Virulence

Insertion elements

Prediction

BBUZS7_RS01690

BBUZS7_RS01740

Genomic Island

LocusTag	DNBias	CDNBias	%GCBias	Product
BBUZS7_RS01690	2	35	-2.238111	Cof-type HAD-IIB family hydrolase
BBUZS7_RS01695	3	33	-1.510909	aminopeptidase
BBUZS7_RS01700	2	33	-2.752498	divergent PAP2 family protein
BBUZS7_RS01705	3	34	-3.313303	hypothetical protein
BBUZS7_RS01710	4	33	-3.419836	membrane protein
BBUZS7_RS01715	4	29	-3.754118	hypothetical protein
BBUZS7_RS01720	3	28	-3.263286	peptide chain release factor 2
BBUZS7_RS01725	2	31	-5.163071	hypothetical protein
BBUZS7_RS01730	1	22	-4.498810	signal recognition particle-docking protein
BBUZS7_RS01735	0	19	-4.263750	hypothetical protein
BBUZS7_RS01740	-1	17	-3.456769	ABC transporter permease

BBUZS7_RS02210

BBUZS7_RS02270

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
BBUZS7_RS02210	2	19	2.011175	DUF58 domain-containing protein
BBUZS7_RS02215	3	10	2.454035	MoxR family ATPase
BBUZS7_RS02220	3	9	2.146260	16S rRNA (guanine(527)-N(7))-methyltransferase
BBUZS7_RS02225	3	12	2.576984	tRNA uridine-5-carboxymethylaminomethyl(34)
BBUZS7_RS02230	4	19	1.341819	tRNA uridine-5-carboxymethylaminomethyl(34)
BBUZS7_RS02235	3	26	1.110379	FlbF protein
BBUZS7_RS02240	2	22	1.423762	flagellar hook-associated protein FlgK
BBUZS7_RS02245	0	15	0.497742	flagellar hook-associated protein 3
BBUZS7_RS02250	0	10	-1.149928	flagellar assembly protein FliW
BBUZS7_RS02255	2	14	-1.331665	carbon storage regulator CsrA
BBUZS7_RS02260	1	17	0.201333	tRNA
BBUZS7_RS02265	2	11	-1.069679	tRNA
BBUZS7_RS02270	2	15	-2.868895	hypothetical protein

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
BBUZS7_RS02215	HTHFIS	39	1e-05	FIS bacterial regulatory protein HTH signature.
>HTHFIS#FIS bacterial regulatory protein HTH signature. Length = 484 Score = 39.4 bits (92), Expect = 1e-05 Identities = 36/143 (25%), Positives = 55/143 (38%), Gaps = 15/143 (10%) Query: 33 KEMIDAILMGLLTDGHVLLEGVPGLAKTL---AIQTVSDVLDLEFKRIQ---FTPDLLPS 86 +E+ + + TD +++ G G K L A+ + F I DL+ S Sbjct: 147 QEIYRVLARLMQTDLTLMITGESGTGKELVARALHDYGKRRNGPFVAINMAAIPRDLIES 206 Query: 87 DLTGNMVYKSA-TGTFKVRKGPVFS----NVILADEINRAPAKVQSALLEAMGERQVT-L 140 +L G K A TG G F + DEI P Q+ LL + + + T + Sbjct: 207 ELFG--HEKGAFTGAQTRSTG-RFEQAEGGTLFLDEIGDMPMDAQTRLLRVLQQGEYTTV 263 Query: 141 GDETHKLPDPFFVLATQNPIEQE 163 G T D V AT ++Q Sbjct: 264 GGRTPIRSDVRIVAATNKDLKQS 286
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
BBUZS7_RS02230	TCRTETOQM	34	0.002	Tetracycline resistance protein TetO/TetQ/TetM family ...
>TCRTETOQM#Tetracycline resistance protein TetO/TetQ/TetM family signature. Length = 639 Score = 33.7 bits (77), Expect = 0.002 Identities = 34/112 (30%), Positives = 48/112 (42%), Gaps = 14/112 (12%) Query: 229 GSVNAGKSSLFNLFLKKDRSIVSSYPGTTRDYIEASFELDGILFNLFDTAGLRDADNFVE 288 GSV+ G + N L++ R I T + I SF+ + N+ DT G D V Sbjct: 34 GSVDKGTTRTDNTLLERQRGI------TIQTGI-TSFQWENTKVNIIDTPGHMDFLAEVY 86 Query: 289 RLGIEKSNSLIKEASLVIYVIDVSSNLTKDDFLFIDSNKSNSKILFVLNKID 340 R S S++ A L+I D T+ LF K +F +NKID Sbjct: 87 R-----SLSVLDGAILLISAKDGVQAQTR--ILFHALRKMGIPTIFFINKID 131
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
BBUZS7_RS02240	FLGHOOKAP1	519	0.0	Flagellar hook-associated protein signature.
>FLGHOOKAP1#Flagellar hook-associated protein signature. Length = 546 Score = 519 bits (1338), Expect = 0.0 Identities = 137/633 (21%), Positives = 259/633 (40%), Gaps = 105/633 (16%) Query: 6 SGIEIGKRSLFAHKDAMNTVGHNLSNATKPGYSRQRVTMKTEIPLYAPQLNRAKKQGQLG 65 S I L A + A+NT +N+S+ GY+RQ M A + G +G Sbjct: 2 SLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIM-------AQANSTLGAGGWVG 54 Query: 66 QGIVVQSIDRVKDELLNTRIIEESHRLGYWTSQDKFISILEDVYNEPEDQSIRKRLNDFW 125 G+ V + R D + ++ + T++ + +S ++++ + S+ ++ DF+ Sbjct: 55 NGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLST-STSSLATQMQDFF 113 Query: 126 ESWHDLANQPQGLAERKIILERGKSFCEGIRNRFHSLERIYIMANDEIKI----TTDEAN 181 S L + + A R+ ++ + EG+ N+F + ++ + ++ I + D+ N Sbjct: 114 TSLQTLVSNAEDPAARQALIGKS----EGLVNQFKTTDQYLRDQDKQVNIAIGASVDQIN 169 Query: 182 NYIRNIANLNKQISKSQAMK--DNPNDLMDARDLMVEKLGNIISVSIENKQDPNEFLIHA 239 NY + IA+LN QIS+ + +PN+L+D RD +V +L I+ V + + + A Sbjct: 170 NYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVSVQDGGTYNITMA 229 Query: 240 EGRHLVQGSIANEF-KLEATNGPTRTRWNIL--WTNN---DKAYLKTGKLGSLLNIRDEE 293 G LVQGS A + + ++ P+RT + N + L TG LG +L R ++ Sbjct: 230 NGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLNTGSLGGILTFRSQD 289 Query: 294 IKNEINELNNIAANIIEIVNEIHEAGRGMDKKNGRSFFSQELKLTDDRGRYDTNGNGQFD 353 + N L +A E N H+AG + G FF+ + N + D Sbjct: 290 LDQTRNTLGQLALAFAEAFNTQHKAGFDANGDAGEDFFAIG------KPAVLQNTKNKGD 343 Query: 354 -SVHIFKINSTNEIFPEEKLGFYGTLKFEATNSNEIVEIPYNAPDTVQDVINRINNSNAQ 412 ++ +++ + + K+ F +++ T A +T V N A Sbjct: 344 VAIGATVTDASAVLATDYKISFDNN-QWQVTRL---------ASNTTFTVTPDANGKVAF 393 Query: 413 VTARINSEGKLEIKAVKEQEDENITFKIKHIEDSGSFLTKYTGILNASGPEGAYDYKNID 472 + G AV + +F +K + D I+N ++ Sbjct: 394 DGLELTFTGTP---AVND------SFTLKPVSD---------AIVNM----------DVL 425 Query: 473 TTD--KLAPKSTYSISPLKNPAAWIKVADIIDSDPSKIASGIKNPTNEISIGDNQAALRI 530 TD K+A S + A DSD + + +N ++G ++ Sbjct: 426 ITDEAKIAMAS-------EEDAG--------DSDNRNGQALLDLQSNSKTVGGAKSF--N 468 Query: 531 SSFGNSQIMIGKNLTLNDYFANTASNIAIKGQISEITKESQSQILKDLTDLRMSISGVNK 590 ++ + IG + T N+ +++++ + Q SISGVN Sbjct: 469 DAYASLVSDIGNKTATLKTSSATQGNV-----VTQLSNQQQ------------SISGVNL 511 Query: 591 DEELANMIEFQQAFIAASKFITVSVELIDTVIN 623 DEE N+ FQQ ++A ++ + + + D +IN Sbjct: 512 DEEYGNLQRFQQYYLANAQVLQTANAIFDALIN 544
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
BBUZS7_RS02245	FLAGELLIN	57	8e-11	Flagellin signature.
>FLAGELLIN#Flagellin signature. Length = 507 Score = 56.6 bits (136), Expect = 8e-11 Identities = 37/133 (27%), Positives = 59/133 (44%), Gaps = 5/133 (3%) Query: 10 TYENFKTSSAEQESKITKLLENLYKGGKRIVKLRNDPTGVTHAIRLDNDIFKLNVYIKNI 69 T N S + S I +L G RI ++D G A R ++I L +N Sbjct: 13 TQNNLNKSQSSLSSAIERL-----SSGLRINSAKDDAAGQAIANRFTSNIKGLTQASRNA 67 Query: 70 DTSKSNLRYTEGYLRSLTNILTRAKEIAIQGASGTYESDDKKMISKEVNALLEDVVAIAN 129 + S + TEG L + N L R +E+++Q +GT D K I E+ LE++ ++N Sbjct: 68 NDGISIAQTTEGALNEINNNLQRVRELSVQATNGTNSDSDLKSIQDEIQQRLEEIDRVSN 127 Query: 130 AKGPDGYSIFSGT 142 +G + S Sbjct: 128 QTQFNGVKVLSQD 140 Score = 30.8 bits (69), Expect = 0.012 Identities = 33/358 (9%), Positives = 87/358 (24%), Gaps = 12/358 (3%) Query: 61 KLNVYIKNIDTSKSNLRYTEGYLRSLTNILTRAKEIAIQGASGTYESDDKKMISKEVNAL 120 + + ++ ID L + TY K + Sbjct: 154 TITIDLQKIDVKSLGLDGFNVNGPKEATVGDLKSSFKNVTGYDTYAVGANKYRVDVNSGA 213 Query: 121 LEDVVAIANAKGPDGYSIFSGTKIDSEAFKVTRENKISKTSKDGAGPQIIKVEYNGNQAE 180 + + +G +A T + T + + + Sbjct: 214 VVTDTTAPTVPDKVYVNAANGQLTTDDAENNTAVDLFKTTKSTAGTAEAKAIAGAIKGGK 273 Query: 181 KKTEVYNDIHMSNNYPGNVIFFLQNQNIISSINTNGFAVKENTKIYIDNIEIGLTAGDTA 240 + + + +S+ I + ++ Sbjct: 274 EGDTFDYK---GVTFTIDTKTGNDGNGKVSTTINGEKVTLTVADITAGAANVDAATLQSS 330 Query: 241 LDIVAKINESSAPVEASIDPVLNSLSIKTTTPHQIWITEEKESNVLQTLGILTKNNDTKL 300 ++ + + LS + + + K+ Sbjct: 331 KNVYTSVVNGQFTFDDKTKNESAKLSDLEANN----AVKGESKITVNGAEYTANAAGDKV 386 Query: 301 PPYNLSSSTEVRSRSIFDALIELRDTLYNNKEELVGSRSLAEIDESLKRLLISVADLGAK 360 + + + + + E + + S ID +L ++ + LGA Sbjct: 387 TLAGKTMFIDKTASGVSTLINEDAAAAKKSTANPLAS-----IDSALSKVDAVRSSLGAI 441 Query: 361 ENRLDRSYERISKEAADMKEDMIQYTDLDVTKAITNLNMASLAYQVSIGISAKIMQTT 418 +NR D + + ++ + D D ++N++ A + Q + A+ Q Sbjct: 442 QNRFDSAITNLGNTVTNLNSARSRIEDADYATEVSNMSKAQILQQAGTSVLAQANQVP 499

BBUZS7_RS02735

BBUZS7_RS02930

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
BBUZS7_RS02735	1	17	3.126565	flagellar biosynthesis protein FliZ
BBUZS7_RS02740	1	18	4.130941	flagellar motor switch protein FliN
BBUZS7_RS02745	1	17	4.930465	flagellar motor switch protein FliM
BBUZS7_RS02750	0	19	4.530761	flagellar basal body-associated protein FliL
BBUZS7_RS02755	-2	20	2.587759	flagellar motor protein MotB
BBUZS7_RS02760	-2	20	1.994362	motility protein A
BBUZS7_RS02765	-2	16	0.460954	flagellar protein FlbD
BBUZS7_RS02770	-2	15	1.231390	flagellar hook protein FlgE
BBUZS7_RS02775	1	16	-0.264266	flagellar hook assembly protein FlgD
BBUZS7_RS02780	5	16	0.740001	flagellar hook-length control protein FliK
BBUZS7_RS02785	6	17	3.013096	flagellar protein
BBUZS7_RS02790	5	18	3.269873	flagellar protein FlbA
BBUZS7_RS02795	4	19	4.263700	flagellar protein export ATPase FliI
BBUZS7_RS02800	6	19	3.992607	flagellar assembly protein FliH
BBUZS7_RS02805	4	19	3.599347	flagellar motor switch protein FliG
BBUZS7_RS02810	2	18	3.486817	flagellar basal body M-ring protein FliF
BBUZS7_RS02815	1	19	2.204966	flagellar hook-basal body complex protein FliE
BBUZS7_RS02820	1	18	1.529780	flagellar basal body rod protein FlgC
BBUZS7_RS02825	1	19	2.794980	flagellar basal body rod protein FlgB
BBUZS7_RS02830	0	18	3.837032	HslU--HslV peptidase ATPase subunit
BBUZS7_RS02835	-1	19	3.278012	ATP-dependent protease subunit HslV
BBUZS7_RS02840	-2	17	2.479007	DNA-protecting protein DprA
BBUZS7_RS02845	-1	16	2.566078	hypothetical protein
BBUZS7_RS02850	-1	15	2.485274	cell division protein FtsZ
BBUZS7_RS02855	0	13	0.737662	cell division protein FtsA
BBUZS7_RS02860	2	13	-0.621829	cell division protein FtsQ/DivIB
BBUZS7_RS02865	3	14	-0.788059	putative lipid II flippase FtsW
BBUZS7_RS02870	1	15	-2.322892	phospho-N-acetylmuramoyl-pentapeptide-
BBUZS7_RS02875	0	17	-3.111035	UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
BBUZS7_RS02880	0	17	-3.410824	hypothetical protein
BBUZS7_RS02885	0	16	-2.788456	16S rRNA (cytosine(1402)-N(4))-methyltransferase
BBUZS7_RS02890	1	17	-3.150989	hypothetical protein
BBUZS7_RS02895	-1	13	-3.050559	hypothetical protein
BBUZS7_RS02900	-1	13	-2.317948	hypothetical protein
BBUZS7_RS02905	-1	13	-2.690593	NAD(+)/NADH kinase
BBUZS7_RS02910	-1	13	-3.330341	chemotaxis protein CheW
BBUZS7_RS02915	0	10	-3.368782	RlmE family RNA methyltransferase
BBUZS7_RS02920	-1	10	-3.229630	polyprenyl synthetase family protein
BBUZS7_RS02925	-1	11	-3.256448	hypothetical protein
BBUZS7_RS02930	1	15	-3.313227	ABC transporter permease

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
BBUZS7_RS02740	FLGMOTORFLIN	103	7e-32	Flagellar motor switch protein FliN signature.
>FLGMOTORFLIN#Flagellar motor switch protein FliN signature. Length = 137 Score = 103 bits (258), Expect = 7e-32 Identities = 40/77 (51%), Positives = 62/77 (80%) Query: 35 NFGLLMDVSMQLTVELGRTERKIKDILGMSEGTIITLDKLAGEPVDILVNGKIVAKGEVV 94 + L+MD+ ++LTVELGRT IK++L +++G+++ LD LAGEP+DIL+NG ++A+GEVV Sbjct: 53 DIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILINGYLIAQGEVV 112 Query: 95 VIDENFGVRITEIIKTK 111 V+ + +GVRIT+II Sbjct: 113 VVADKYGVRITDIITPS 129
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
BBUZS7_RS02745	FLGMOTORFLIM	458	e-165	Flagellar motor switch protein FliM signature.
>FLGMOTORFLIM#Flagellar motor switch protein FliM signature. Length = 344 Score = 458 bits (1181), Expect = e-165 Identities = 195/345 (56%), Positives = 259/345 (75%), Gaps = 9/345 (2%) Query: 8 LSQDDIDSLLESINSSESLSLDESLSNVISSPTGKKQKVKVYDFKRPDKFSKEQVRTVSS 67 LSQD+ID LL +I+S ++ D + P +K+ +YDF+RPDKFSKEQ+RT+S Sbjct: 5 LSQDEIDQLLTAISSGDASIED-------ARPISDTRKITLYDFRRPDKFSKEQMRTLSL 57 Query: 68 FHEAFARYTTTSLSALLRKMVHVHVASVDQLTYEEFIRSIPNPTTLAIINMDPLKGSAIF 127 HE FAR TTTSLSA LR MVHVHVASVDQLTYEEFIRSIP P+TLA+I MDPLKG+A+ Sbjct: 58 MHETFARLTTTSLSAQLRSMVHVHVASVDQLTYEEFIRSIPTPSTLAVITMDPLKGNAVL 117 Query: 128 EVDPTIAFAIVDRLFGGDGDTIKDKSRDLTEIEQSVMESVIIRILANMREAWSQVVDLRP 187 EVDP+I F+I+DRLFGG G K + RDLT+IE SVME VI+RILAN+RE+W+QV+DLRP Sbjct: 118 EVDPSITFSIIDRLFGGTGQAAKVQ-RDLTDIENSVMEGVIVRILANVRESWTQVIDLRP 176 Query: 188 RFGHIEVNPQFAQIVPPTEMIILVTLEVKIGKVEGLMNFCLPYITIEPIVSKLSTRYWHS 247 R G IE NPQFAQIVPP+EM++LVTLE K+G+ EG+MNFC+PYITIEPI+SKLS+++W S Sbjct: 177 RLGQIETNPQFAQIVPPSEMVVLVTLETKVGEEEGMMNFCIPYITIEPIISKLSSQFWFS 236 Query: 248 LIGVGTTSENLDALREKLENTAMPLVAEIGEVKLKVREILSLDKGDVLNLESSLINKDLT 307 + +T++ + LR+KL M +VAE+G ++L VR+IL L GD++ L + + Sbjct: 237 SVRRSSTTQYMGVLRDKLSTVDMDVVAEVGSLRLSVRDILGLRVGDIIRLHDTHVGDPFV 296 Query: 308 LKVGTKEKFKCRMGLMGNKVSVQITEKIGDIKGFDLLKELTEEVE 352 L +G ++KF C+ G++G K++ QI E+I D +EL+ + E Sbjct: 297 LSIGNRKKFLCQPGVVGKKIAAQILERIESTSQED-FEELSADEE 340
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
BBUZS7_RS02755	OMPADOMAIN	55	8e-11	OMPA domain signature.
>OMPADOMAIN#OMPA domain signature. Length = 346 Score = 54.6 bits (131), Expect = 8e-11 Identities = 28/132 (21%), Positives = 57/132 (43%), Gaps = 21/132 (15%) Query: 124 ISLAADAFFDSASADVKLEENRDSIQKIASFIGFLSPRGYNFKIEGHTDNIDTDVNGPWK 183 +L +D F+ A +K E + ++ ++ S + L P+ + + G+TD I +D Sbjct: 215 FTLKSDVLFNFNKATLK-PEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSD-----A 268 Query: 184 SNWELSAARSVNMLEHILNYLDQSDVKRIENNFEVSGFGGSRPIATDDT---------PE 234 N LS R+ + +++YL + + G G S P+ + + Sbjct: 269 YNQGLSERRA----QSVVDYLISKGIPA--DKISARGMGESNPVTGNTCDNVKQRAALID 322 Query: 235 GRAYNRRIDILI 246 A +RR++I + Sbjct: 323 CLAPDRRVEIEV 334
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
BBUZS7_RS02770	FLGHOOKAP1	51	4e-09	Flagellar hook-associated protein signature.
>FLGHOOKAP1#Flagellar hook-associated protein signature. Length = 546 Score = 51.5 bits (123), Expect = 4e-09 Identities = 18/89 (20%), Positives = 33/89 (37%), Gaps = 13/89 (14%) Query: 4 SLYSGVSGLQNHQTRMDVVGNNIANVNTIGFKKGRVNFQDMISQSISGASRPTDARGGTN 63 + + +SGL Q ++ NNI++ N G+ + I + T GG Sbjct: 3 LINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTT---------IMAQANSTLGAGGW- 52 Query: 64 PKQVGLGMNVASIDTIHTQGAFQSTQKAS 92 VG G+ V+ + + + A Sbjct: 53 ---VGNGVYVSGVQREYDAFITNQLRAAQ 78 Score = 40.7 bits (95), Expect = 1e-05 Identities = 9/48 (18%), Positives = 28/48 (58%) Query: 394 IRSGVLEMANVDLAEQFTDMIVTQRGFQANAKTITTSDQLLQELVRLK 441 + + ++ V+L E++ ++ Q+ + ANA+ + T++ + L+ ++ Sbjct: 499 LSNQQQSISGVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR 546
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
BBUZS7_RS02780	FLGHOOKFLIK	39	2e-05	Flagellar hook-length control protein signature.
>FLGHOOKFLIK#Flagellar hook-length control protein signature. Length = 375 Score = 39.4 bits (91), Expect = 2e-05 Identities = 24/82 (29%), Positives = 45/82 (54%), Gaps = 5/82 (6%) Query: 281 KLVLKPKELGSIRINLNLDSNNNLLGKIVVDNQNVKMLFDQNMHSLNKMLGESGFNASLN 340 +L L P++LG ++I+L +D N + ++V +Q+V+ + + L L ESG + Sbjct: 260 ELRLHPQDLGEVQISLKVDDNQAQI-QMVSPHQHVRAALEAALPVLRTQLAESGIQLGQS 318 Query: 341 LFLAGENLNSFTGDFKDDSKDQ 362 ++GE SF+G + S+ Q Sbjct: 319 -NISGE---SFSGQQQAASQQQ 336
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
BBUZS7_RS02785	TYPE4SSCAGX	29	0.019	Type IV secretion system CagX conjugation protein si...
>TYPE4SSCAGX#Type IV secretion system CagX conjugation protein signature. Length = 522 Score = 28.6 bits (63), Expect = 0.019 Identities = 34/143 (23%), Positives = 66/143 (46%), Gaps = 20/143 (13%) Query: 39 RYFPEFVRTKLLGETSLVFDHNSNIILDEAR--LVKEREAIDIKNQQIEKLKEDLKLKED 96 R + EF++TK L+ D L+E + L KE+EA + + Q+ +K K + + +E Sbjct: 120 RDYQEFLKTK-----KLIVDAPDPKELEEQKKALEKEKEAKE-QAQKAQKDKREKRKEER 173 Query: 97 SLNKLEFELKQKQKDLDLKQKVIDDIINKYNDEEANILQTAVYLMNMPPEDAVKRLEDLN 156 + N+ E + + + N N L + D ++RLED+ Sbjct: 174 AKNRANLE------------NLTNAMSNPQNLSNNKNLSELIKQQRENELDQMERLEDMQ 221 Query: 157 PELAISYMRKIEELSKKEGRLSI 179 + + +++IEEL+KK+ ++ Sbjct: 222 EQAQANALKQIEELNKKQAEEAV 244
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
BBUZS7_RS02800	FLGFLIH	47	3e-08	Flagellar assembly protein FliH signature.
>FLGFLIH#Flagellar assembly protein FliH signature. Length = 228 Score = 46.7 bits (110), Expect = 3e-08 Identities = 43/197 (21%), Positives = 96/197 (48%), Gaps = 22/197 (11%) Query: 115 KESIETESNAEIER-LAREYEEKLKTDLDIAIAKGREEGYSKGY--------ESGFEDFD 165 +E+I E+ +E+ LA+ + + IA+GR++G+ +GY E G + Sbjct: 29 EETIIEEAEPSLEQQLAQLQMQAHEQGYQAGIAEGRQQGHKQGYQEGLAQGLEQGLAEAK 88 Query: 166 KVMRKLHAIIASLIAERKGILESSSGQIVSLVMQIAIKVIKRITDSQKDI----VLENVN 221 +HA + L++E + L++ I S +MQ+A++ +++ + +++ + Sbjct: 89 SQQAPIHARMQQLVSEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQ 148 Query: 222 EVLKR---VKDKTQITIRVNLDDLDIVRHKKSDFISRFDIIENLEIIEDPNIGKGGCIIE 278 ++L++ K Q +RV+ DDL V +S + + DP + GGC + Sbjct: 149 QLLQQEPLFSGKPQ--LRVHPDDLQRVDDMLGATLS----LHGWRLRGDPTLHPGGCKVS 202 Query: 279 TNFGEIDARISSQLDKI 295 + G++DA ++++ ++ Sbjct: 203 ADEGDLDASVATRWQEL 219
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
BBUZS7_RS02805	FLGMOTORFLIG	427	e-153	Flagellar motor switch protein FliG signature.
>FLGMOTORFLIG#Flagellar motor switch protein FliG signature. Length = 344 Score = 427 bits (1100), Expect = e-153 Identities = 344/344 (100%), Positives = 344/344 (100%) Query: 1 MEEKKEKEILDVSALTGKQKAAILLVSIGSEISSKVFKYLSQEEIESLTFEIAKLETITS 60 MEEKKEKEILDVSALTGKQKAAILLVSIGSEISSKVFKYLSQEEIESLTFEIAKLETITS Sbjct: 1 MEEKKEKEILDVSALTGKQKAAILLVSIGSEISSKVFKYLSQEEIESLTFEIAKLETITS 60 Query: 61 ELKDNVLLEFKELMMAQEFIQKGGIDYARELLEKSLGTQKAVDIINNLGSALQSRPFEFV 120 ELKDNVLLEFKELMMAQEFIQKGGIDYARELLEKSLGTQKAVDIINNLGSALQSRPFEFV Sbjct: 61 ELKDNVLLEFKELMMAQEFIQKGGIDYARELLEKSLGTQKAVDIINNLGSALQSRPFEFV 120 Query: 121 RRADPANILNFIQQEHPQTIALILSYLDPQKASFILSSLPTEVQTNVARRIALMDRTSPE 180 RRADPANILNFIQQEHPQTIALILSYLDPQKASFILSSLPTEVQTNVARRIALMDRTSPE Sbjct: 121 RRADPANILNFIQQEHPQTIALILSYLDPQKASFILSSLPTEVQTNVARRIALMDRTSPE 180 Query: 181 VVREVERVLEKKLASLSSEDYTSAGGVDNVVEIINMADRKTEKFIIESLEEEDPELAEEI 240 VVREVERVLEKKLASLSSEDYTSAGGVDNVVEIINMADRKTEKFIIESLEEEDPELAEEI Sbjct: 181 VVREVERVLEKKLASLSSEDYTSAGGVDNVVEIINMADRKTEKFIIESLEEEDPELAEEI 240 Query: 241 KKKMFVFEDIVLLDDRSIQRVLREIDGQELAKALKSVDIPVQEKIFKNMSKRAASMLKED 300 KKKMFVFEDIVLLDDRSIQRVLREIDGQELAKALKSVDIPVQEKIFKNMSKRAASMLKED Sbjct: 241 KKKMFVFEDIVLLDDRSIQRVLREIDGQELAKALKSVDIPVQEKIFKNMSKRAASMLKED 300 Query: 301 MEFLGPTRRKDVEESQQKIVSLIRKLEEQGEIVISRGGEEDVLV 344 MEFLGPTRRKDVEESQQKIVSLIRKLEEQGEIVISRGGEEDVLV Sbjct: 301 MEFLGPTRRKDVEESQQKIVSLIRKLEEQGEIVISRGGEEDVLV 344
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
BBUZS7_RS02810	FLGMRINGFLIF	162	1e-45	Flagellar M-ring protein signature.
>FLGMRINGFLIF#Flagellar M-ring protein signature. Length = 559 Score = 162 bits (412), Expect = 1e-45 Identities = 112/565 (19%), Positives = 222/565 (39%), Gaps = 55/565 (9%) Query: 23 QKIALGLIIFFVILALVFLIGFSTKSQSIALF-GVEIKDQYLLDRISQRLDRENVKYFLS 81 +I L + + +V ++ ++ LF + +D I +L + N+ Y + Sbjct: 23 PRIPLIVAGSAAVAIVVAMVLWAKTPDYRTLFSNLSDQD---GGAIVAQLTQMNIPYRFA 79 Query: 82 SDGRIYLDDEKLAKKMRAILVREELVPVHMDPWALFDIDRWTITDFERSINLRRSITRAV 141 + ++R L ++ L + L D +++ I+ F +N +R++ + Sbjct: 80 NGSGAIEVPADKVHELRLRLAQQGLPKGGAVGFELLDQEKFGISQFSEQVNYQRALEGEL 139 Query: 142 EQHIVALDDVDAVSVNLVMPEKALFKESQEPVKASVRITPRPGSDIITNRKKVEGLVKLI 201 + I L V + V+L MP+ +LF Q+ ASV +T PG + + ++ +V L+ Sbjct: 140 ARTIETLGPVKSARVHLAMPKPSLFVREQKSPSASVTVTLEPGRAL--DEGQISAVVHLV 197 Query: 202 QYAIEGLESDNIAIVDNSGTILNDFSNLDGIDRIDLAEKERKLKLKYEAMLRGEIDSALS 261 A+ GL N+ +VD SG +L SN G DL + + K E+ ++ I++ LS Sbjct: 198 SSAVAGLPPGNVTLVDQSGHLL-TQSNTSG---RDLNDAQLKFANDVESRIQRRIEAILS 253 Query: 262 KVLSVDRFMIARVNVKLDTSKETTESKEYAPIELQSQDPKASYNTRKVSDSTIISSQTQK 321 ++ A+V +LD + + + Y+P KA+ +R+++ S + + Sbjct: 254 PIVGNGNV-HAQVTAQLDFANKEQTEEHYSP---NGDASKATLRSRQLNISEQVGAGYPG 309 Query: 322 KEYQGQGYSPWGPPGQEGNTPPEYQDLSD-------------ITGKYNESQEIKNVALNE 368 P P TPP Q + + + E N ++ Sbjct: 310 GVPGALSNQPAPPNEAPIATPPTNQQNAQNTPQTSTSTNSNSAGPRSTQRNETSNYEVDR 369 Query: 369 KKSTSEKEPARIVGVSLGIFVDGIWNFVYDEKGDFVIENGMRKREYKPMALEEIKNIEDV 428 ++ I +S+ + V+ + + P+ +++K IED+ Sbjct: 370 TIRHTKMNVGDIERLSVAVVVNY---------------KTLADGKPLPLTADQMKQIEDL 414 Query: 429 LQSSFEYKPERGDSITVRNISFDRMNEFREIDENYFASER--FKYFLFIASIVFSLLILV 486 + + + +RGD++ V N F ++ E F ++ L + L++ Sbjct: 415 TREAMGFSDKRGDTLNVVNSPFSAVDN--TGGELPFWQQQSFIDQLLAAGRWLLVLVVAW 472 Query: 487 FTIFFAISRELERRRRLREEELAKQAHLRRQQALMDG-----GDDIGVDDVVGGIREGDE 541 A+ +L RR EE A Q + +Q + D + R G E Sbjct: 473 ILWRKAVRPQLTRR---VEEAKAAQEQAQVRQETEEAVEVRLSKDEQLQQRRANQRLGAE 529 Query: 542 LQSNA-ELLAREKPEDVAKLIRTWL 565 + S ++ P VA +IR W+ Sbjct: 530 VMSQRIREMSDNDPRVVALVIRQWM 554
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
BBUZS7_RS02815	FLGHOOKFLIE	86	2e-25	Flagellar hook-basal body complex protein FliE signa...
>FLGHOOKFLIE#Flagellar hook-basal body complex protein FliE signature. Length = 103 Score = 85.9 bits (212), Expect = 2e-25 Identities = 15/71 (21%), Positives = 38/71 (53%) Query: 40 TFKDVLINSITDVNKSQLNVSKVTEQAILKPSSIDVHDVVIAMSKANMNLSILKAVVERG 99 +F L ++ ++ +Q E+ L + ++DV+ M KA++++ + V + Sbjct: 32 SFAGQLHAALDRISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKL 91 Query: 100 VKAYQDIINIR 110 V AYQ++++++ Sbjct: 92 VAAYQEVMSMQ 102
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
BBUZS7_RS02820	FLGHOOKAP1	48	3e-09	Flagellar hook-associated protein signature.
>FLGHOOKAP1#Flagellar hook-associated protein signature. Length = 546 Score = 47.6 bits (113), Expect = 3e-09 Identities = 21/80 (26%), Positives = 35/80 (43%), Gaps = 15/80 (18%) Query: 5 SSINVASTGLTAQRLRIDVISNNIANVSTSRTPDGGPYRRQRIIFAPRVNNPYWKGPFIP 64 S IN A +GL A + ++ SNNI++ + + Y RQ I A N Sbjct: 2 SLINNAMSGLNAAQAALNTASNNISSYNVA------GYTRQTTIMAQ--ANSTLGA---- 49 Query: 65 DYLDNGIGQGVRVASIEKDK 84 +G GV V+ ++++ Sbjct: 50 ---GGWVGNGVYVSGVQREY 66 Score = 44.2 bits (104), Expect = 5e-08 Identities = 11/46 (23%), Positives = 23/46 (50%) Query: 104 KKGYVELPNVNLVEEMVDMISASRAYEANSTVINSSKSMFRSALAI 149 + VNL EE ++ + Y AN+ V+ ++ ++F + + I Sbjct: 500 SNQQQSISGVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINI 545
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
BBUZS7_RS02830	HTHFIS	31	0.010	FIS bacterial regulatory protein HTH signature.
>HTHFIS#FIS bacterial regulatory protein HTH signature. Length = 484 Score = 31.0 bits (70), Expect = 0.010 Identities = 13/70 (18%), Positives = 30/70 (42%), Gaps = 15/70 (21%) Query: 20 KYIIGQDEAKKLVSIALVNRYIRSRLPKEIKDEVMPKNIIMIGSTGIGKTEIAR---RLS 76 ++G+ A + + ++ R +++ L +++ G +G GK +AR Sbjct: 137 MPLVGRSAAMQEI-YRVLARLMQTDLT-----------LMITGESGTGKELVARALHDYG 184 Query: 77 KLIKAPFIKV 86 K PF+ + Sbjct: 185 KRRNGPFVAI 194
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
BBUZS7_RS02845	SYCDCHAPRONE	29	0.012	Gram-negative bacterial type III secretion SycD cha...
>SYCDCHAPRONE#Gram-negative bacterial type III secretion SycD chaperone signature. Length = 168 Score = 28.7 bits (64), Expect = 0.012 Identities = 16/91 (17%), Positives = 34/91 (37%), Gaps = 2/91 (2%) Query: 69 ARFFNLIGLEFFKLGQYGPAIEYFAKNLEINPNNYLSHFYIGVASYNLAKNLRVKDEVEK 128 +RFF +G +GQY AI ++ ++ F+ + + + Sbjct: 70 SRFFLGLGACRQAMGQYDLAIHSYSYGAIMDIKEPRFPFHAAECLLQKGELAEAESGLFL 129 Query: 129 YI-ILAENSFLKSLSIR-DDFKDSLFAISNM 157 ++A+ + K LS R +++ M Sbjct: 130 AQELIADKTEFKELSTRVSSMLEAIKLKKEM 160
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
BBUZS7_RS02855	SHAPEPROTEIN	56	8e-11	Bacterial cell shape determinant MreB/Mbl protein s...
>SHAPEPROTEIN#Bacterial cell shape determinant MreB/Mbl protein signature. Length = 347 Score = 55.9 bits (135), Expect = 8e-11 Identities = 50/226 (22%), Positives = 87/226 (38%), Gaps = 24/226 (10%) Query: 160 TGSSSSSQNLVR-CVNRAGFAVDEVVLGSLASSYATLSKEEREMGVLFIDMGKGTTDIIL 218 G++ + +R AG ++ +A++ G + +D+G GTT++ + Sbjct: 116 VGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATGSMVVDIGGGTTEVAV 175 Query: 219 YIDGSPYYTGVIPIGVNRVTLDIAQVWK------VPEDVAENIKITAGIAHPSILESQME 272 Y+ + IG +R I + + E AE IK G A+P ++E Sbjct: 176 ISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERIKHEIGSAYPGDEVREIE 235 Query: 273 TVIIPNLGTRPPQ--EKSRKELSVIINSRLREIFEMMKAEI------LKRGLYNKINGGI 324 V NL P+ + E+ + L I + + L + + G+ Sbjct: 236 -VRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALEQCPPELASDISER---GM 291 Query: 325 VLTGGGALFPGISNLIEEVFNYPARIGL-PMSINGIGE----EHID 365 VLTGGGAL + L+ E P + P++ G E ID Sbjct: 292 VLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALEMID 337

BBUZS7_RS03355

BBUZS7_RS07015

Genomic Island

LocusTag	DNBias	CDNBias	%GCBias	Product
BBUZS7_RS03355	2	11	-0.163898	dicarboxylate/amino acid:cation symporter
BBUZS7_RS03360	1	12	0.958976	proline--tRNA ligase
BBUZS7_RS03365	3	12	2.023306	DUF2259 domain-containing protein
BBUZS7_RS03370	4	11	2.456262	hypothetical protein
BBUZS7_RS03375	3	11	2.395789	DUF3996 domain-containing protein
BBUZS7_RS03380	4	11	0.998496	DUF3996 domain-containing protein
BBUZS7_RS03385	3	9	0.513145	mannose-6-phosphate isomerase, class I
BBUZS7_RS07015	3	12	-0.237826	PTS transporter subunit EIIA

BBUZS7_RS04535

BBUZS7_RS04600

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
BBUZS7_RS04535	2	29	-2.899131	PTS transporter subunit EIIA
BBUZS7_RS04540	2	29	-3.684962	1-phosphofructokinase
BBUZS7_RS04545	2	26	-3.735708	hypothetical protein
BBUZS7_RS04555	2	22	-3.339057	*exodeoxyribonuclease V subunit alpha
BBUZS7_RS04560	2	16	-2.537130	exodeoxyribonuclease V subunit beta
BBUZS7_RS04565	1	14	-1.572590	exodeoxyribonuclease V subunit gamma
BBUZS7_RS04570	1	10	0.187128	nicotinate phosphoribosyltransferase
BBUZS7_RS04575	2	9	0.103030	glucose-6-phosphate dehydrogenase
BBUZS7_RS04580	2	13	0.643448	Na+/H+ antiporter NhaC family protein
BBUZS7_RS04585	3	17	-0.142035	Na+/H+ antiporter NhaC family protein
BBUZS7_RS04590	3	22	-0.373073	ABC transporter substrate-binding protein
BBUZS7_RS04595	1	14	1.658717	ABC transporter permease
BBUZS7_RS04600	2	13	2.123009	ABC transporter permease

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
BBUZS7_RS04555	MYCMG045	30	0.020	Hypothetical mycoplasma lipoprotein (MG045) signature.
>MYCMG045#Hypothetical mycoplasma lipoprotein (MG045) signature. Length = 483 Score = 30.4 bits (68), Expect = 0.020 Identities = 30/113 (26%), Positives = 58/113 (51%), Gaps = 12/113 (10%) Query: 76 LLAKDIQNTIIFTKDNLEKTNKSYNKLIKILKGLETFGNLETIKNIVLLLK--KNNILME 133 L+ +D+ + I +++ NL+K++ S +K+ + F +++IK I K KNN L+ Sbjct: 84 LIERDLLSPIDWSQFNLKKSSSSSDKVNNASDAKDLF--IDSIKEISQQTKDSKNNELLH 141 Query: 134 FNKLKITTPLILENNIYIYTQKNYREEEE---LIKQIIKRLENHKSELNDNKI 183 + P L+N +++Y + E E+ +IK + HK NDN++ Sbjct: 142 W-----AVPYFLQNLVFVYRGEKISELEQENVSWTDVIKAIVKHKDRFNDNRL 189
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
BBUZS7_RS04590	MYCMG045	34	6e-04	Hypothetical mycoplasma lipoprotein (MG045) signature.
>MYCMG045#Hypothetical mycoplasma lipoprotein (MG045) signature. Length = 483 Score = 34.3 bits (78), Expect = 6e-04 Identities = 25/120 (20%), Positives = 59/120 (49%), Gaps = 4/120 (3%) Query: 1 MKKIFILIVILTTFACTNKDTITLNVFNWAEYIDETLLDQFEKENNIKINYEIFHNNEEM 60 +K F + + + ++ + T + N+ YI LL++ ++++ + + + +NE++ Sbjct: 5 LKYCFFSLFVSLSSILSSCGSTTFVLANFESYISPLLLERVQEKH--PLTFLTYPSNEKL 62 Query: 61 MAKFNNTKNYYDIIVPSEYLIQELIDEGKIEKLDYSKLPNVTKNITQNLTNLEHDPGNLY 120 + F N N Y + V S Y + ELI+ + +D+S+ + + + N D +L+ Sbjct: 63 INGFAN--NTYSVAVASTYAVSELIERDLLSPIDWSQFNLKKSSSSSDKVNNASDAKDLF 120

BBUZS7_RS04865

BBUZS7_RS04955

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
BBUZS7_RS04865	3	20	2.728987	signal recognition particle protein
BBUZS7_RS04870	1	20	1.320938	30S ribosomal protein S16
BBUZS7_RS04875	1	21	0.566348	KH domain-containing protein
BBUZS7_RS04880	2	21	0.009588	16S rRNA processing protein RimM
BBUZS7_RS04885	2	22	0.300401	tRNA (guanosine(37)-N1)-methyltransferase TrmD
BBUZS7_RS04890	0	26	0.134792	50S ribosomal protein L19
BBUZS7_RS04895	-2	15	-2.580238	hypothetical protein
BBUZS7_RS04900	-1	10	-2.486331	pantetheine-phosphate adenylyltransferase
BBUZS7_RS04905	-1	10	-3.014763	50S ribosomal protein L32
BBUZS7_RS04910	-1	9	-2.886474	acyl carrier protein
BBUZS7_RS04915	-2	10	-2.729892	ribonuclease III
BBUZS7_RS04920	0	10	-1.787156	CCA tRNA nucleotidyltransferase
BBUZS7_RS04925	2	16	-0.740782	hypothetical protein
BBUZS7_RS04930	3	14	-0.621070	hypothetical protein
BBUZS7_RS04945	2	14	0.824439	**endolytic transglycosylase MltG
BBUZS7_RS04950	3	14	0.990876	DNA primase
BBUZS7_RS04955	3	14	0.946452	RNA polymerase sigma factor RpoD

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
BBUZS7_RS04900	LPSBIOSNTHSS	198	4e-68	Lipopolysaccharide core biosynthesis protein signat...
>LPSBIOSNTHSS#Lipopolysaccharide core biosynthesis protein signature. Length = 166 Score = 198 bits (505), Expect = 4e-68 Identities = 57/157 (36%), Positives = 91/157 (57%), Gaps = 3/157 (1%) Query: 4 AVFPGSFDPITWGHIDLIKRSLAIFDKVIVLVAKNKSKKYFLSDIERFSLTKDVISSLNF 63 A++PGSFDPIT+GH+D+I+R +FD+V V V +N +K+ S ER I+ L Sbjct: 3 AIYPGSFDPITFGHLDIIERGCRLFDQVYVAVLRNPNKQPMFSVQERLEQIAKAIAHL-- 60 Query: 64 SNVLVDRYSGFIVDYALINSIKFIVRGIRAFNDFDIEFERYLVNNKLNFEIDTIFLPSSA 123 N VD + G V+YA I+RG+R +DF++E + N L +++T+FL +S Sbjct: 61 PNAQVDSFEGLTVNYARQRQAGAILRGLRVLSDFELELQMANTNKTLASDLETVFLTTST 120 Query: 124 EHLYVRSDFVKELMLKKDVDLSNFVPELVFNRLKSKF 160 E+ ++ S VKE+ + ++ +FVP V L +F Sbjct: 121 EYSFLSSSLVKEVA-RFGGNVEHFVPSHVAAALYDQF 156

BBUZS7_RS05405

BBUZS7_RS05500

Pathogenicity Island (biased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
BBUZS7_RS05405	2	17	4.080350	transcription termination/antitermination
BBUZS7_RS05410	0	15	2.869129	translation initiation factor IF-2
BBUZS7_RS05415	-1	15	0.140375	30S ribosome-binding factor RbfA
BBUZS7_RS05420	-1	15	-0.016237	tRNA pseudouridine(55) synthase TruB
BBUZS7_RS05425	0	15	0.516050	30S ribosomal protein S15
BBUZS7_RS05430	0	14	0.137054	polyribonucleotide nucleotidyltransferase
BBUZS7_RS05435	1	14	-1.590877	hypothetical protein
BBUZS7_RS05440	2	11	-1.185323	YjgP/YjgQ family permease
BBUZS7_RS05445	2	12	-0.480500	YjgP/YjgQ family permease
BBUZS7_RS05450	3	12	-0.867593	tRNA guanosine(34) transglycosylase Tgt
BBUZS7_RS05455	3	12	-1.588602	murein biosynthesis integral membrane protein
BBUZS7_RS05460	2	10	-1.372080	HEAT repeat domain-containing protein
BBUZS7_RS05465	3	11	-1.987991	bifunctional phosphopantothenoylcysteine
BBUZS7_RS07235	1	15	-1.828702	DUF997 family protein
BBUZS7_RS05470	0	14	-1.563177	sodium/pantothenate symporter
BBUZS7_RS05475	0	14	-1.069529	RluA family pseudouridine synthase
BBUZS7_RS05480	-1	13	-1.038948	hypothetical protein
BBUZS7_RS05485	-1	13	-1.176760	UDP-N-acetylmuramate--L-alanine ligase
BBUZS7_RS05490	0	14	-0.363189	YicC family protein
BBUZS7_RS05495	-1	12	-0.157914	AAA family ATPase
BBUZS7_RS05500	3	14	-2.052474	DNA-directed RNA polymerase subunit omega

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
BBUZS7_RS05410	TCRTETOQM	76	4e-16	Tetracycline resistance protein TetO/TetQ/TetM family ...
>TCRTETOQM#Tetracycline resistance protein TetO/TetQ/TetM family signature. Length = 639 Score = 75.7 bits (186), Expect = 4e-16 Identities = 41/144 (28%), Positives = 60/144 (41%), Gaps = 22/144 (15%) Query: 385 ITIMGHVDHGKTKLLSVL------------------QNIDINQTESGGITQHIGAYTIVY 426 I ++ HVD GKT L L + + GIT G + + Sbjct: 6 IGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTDNTLLERQRGITIQTGITSFQW 65 Query: 427 NDREITFLDTPGHEAFTMMRSRGAQVTDIIVLVVSAIDGVMPQTIEAINHAKEANVPIIV 486 + ++ +DTPGH F R V D +L++SA DGV QT + ++ +P I Sbjct: 66 ENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAKDGVQAQTRILFHALRKMGIPTIF 125 Query: 487 AINKIDLPDSNPDK----IKHQLS 506 INKID + IK +LS Sbjct: 126 FINKIDQNGIDLSTVYQDIKEKLS 149

BBUZS7_RS02215

BBUZS7_RS02245

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
BBUZS7_RS02215	3	10	2.454035	MoxR family ATPase
BBUZS7_RS02220	3	9	2.146260	16S rRNA (guanine(527)-N(7))-methyltransferase
BBUZS7_RS02225	3	12	2.576984	tRNA uridine-5-carboxymethylaminomethyl(34)
BBUZS7_RS02230	4	19	1.341819	tRNA uridine-5-carboxymethylaminomethyl(34)
BBUZS7_RS02235	3	26	1.110379	FlbF protein
BBUZS7_RS02240	2	22	1.423762	flagellar hook-associated protein FlgK
BBUZS7_RS02245	0	15	0.497742	flagellar hook-associated protein 3

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
BBUZS7_RS02215	HTHFIS	39	1e-05	FIS bacterial regulatory protein HTH signature.
>HTHFIS#FIS bacterial regulatory protein HTH signature. Length = 484 Score = 39.4 bits (92), Expect = 1e-05 Identities = 36/143 (25%), Positives = 55/143 (38%), Gaps = 15/143 (10%) Query: 33 KEMIDAILMGLLTDGHVLLEGVPGLAKTL---AIQTVSDVLDLEFKRIQ---FTPDLLPS 86 +E+ + + TD +++ G G K L A+ + F I DL+ S Sbjct: 147 QEIYRVLARLMQTDLTLMITGESGTGKELVARALHDYGKRRNGPFVAINMAAIPRDLIES 206 Query: 87 DLTGNMVYKSA-TGTFKVRKGPVFS----NVILADEINRAPAKVQSALLEAMGERQVT-L 140 +L G K A TG G F + DEI P Q+ LL + + + T + Sbjct: 207 ELFG--HEKGAFTGAQTRSTG-RFEQAEGGTLFLDEIGDMPMDAQTRLLRVLQQGEYTTV 263 Query: 141 GDETHKLPDPFFVLATQNPIEQE 163 G T D V AT ++Q Sbjct: 264 GGRTPIRSDVRIVAATNKDLKQS 286
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
BBUZS7_RS02230	TCRTETOQM	34	0.002	Tetracycline resistance protein TetO/TetQ/TetM family ...
>TCRTETOQM#Tetracycline resistance protein TetO/TetQ/TetM family signature. Length = 639 Score = 33.7 bits (77), Expect = 0.002 Identities = 34/112 (30%), Positives = 48/112 (42%), Gaps = 14/112 (12%) Query: 229 GSVNAGKSSLFNLFLKKDRSIVSSYPGTTRDYIEASFELDGILFNLFDTAGLRDADNFVE 288 GSV+ G + N L++ R I T + I SF+ + N+ DT G D V Sbjct: 34 GSVDKGTTRTDNTLLERQRGI------TIQTGI-TSFQWENTKVNIIDTPGHMDFLAEVY 86 Query: 289 RLGIEKSNSLIKEASLVIYVIDVSSNLTKDDFLFIDSNKSNSKILFVLNKID 340 R S S++ A L+I D T+ LF K +F +NKID Sbjct: 87 R-----SLSVLDGAILLISAKDGVQAQTR--ILFHALRKMGIPTIFFINKID 131
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
BBUZS7_RS02240	FLGHOOKAP1	519	0.0	Flagellar hook-associated protein signature.
>FLGHOOKAP1#Flagellar hook-associated protein signature. Length = 546 Score = 519 bits (1338), Expect = 0.0 Identities = 137/633 (21%), Positives = 259/633 (40%), Gaps = 105/633 (16%) Query: 6 SGIEIGKRSLFAHKDAMNTVGHNLSNATKPGYSRQRVTMKTEIPLYAPQLNRAKKQGQLG 65 S I L A + A+NT +N+S+ GY+RQ M A + G +G Sbjct: 2 SLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIM-------AQANSTLGAGGWVG 54 Query: 66 QGIVVQSIDRVKDELLNTRIIEESHRLGYWTSQDKFISILEDVYNEPEDQSIRKRLNDFW 125 G+ V + R D + ++ + T++ + +S ++++ + S+ ++ DF+ Sbjct: 55 NGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLST-STSSLATQMQDFF 113 Query: 126 ESWHDLANQPQGLAERKIILERGKSFCEGIRNRFHSLERIYIMANDEIKI----TTDEAN 181 S L + + A R+ ++ + EG+ N+F + ++ + ++ I + D+ N Sbjct: 114 TSLQTLVSNAEDPAARQALIGKS----EGLVNQFKTTDQYLRDQDKQVNIAIGASVDQIN 169 Query: 182 NYIRNIANLNKQISKSQAMK--DNPNDLMDARDLMVEKLGNIISVSIENKQDPNEFLIHA 239 NY + IA+LN QIS+ + +PN+L+D RD +V +L I+ V + + + A Sbjct: 170 NYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVSVQDGGTYNITMA 229 Query: 240 EGRHLVQGSIANEF-KLEATNGPTRTRWNIL--WTNN---DKAYLKTGKLGSLLNIRDEE 293 G LVQGS A + + ++ P+RT + N + L TG LG +L R ++ Sbjct: 230 NGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLNTGSLGGILTFRSQD 289 Query: 294 IKNEINELNNIAANIIEIVNEIHEAGRGMDKKNGRSFFSQELKLTDDRGRYDTNGNGQFD 353 + N L +A E N H+AG + G FF+ + N + D Sbjct: 290 LDQTRNTLGQLALAFAEAFNTQHKAGFDANGDAGEDFFAIG------KPAVLQNTKNKGD 343 Query: 354 -SVHIFKINSTNEIFPEEKLGFYGTLKFEATNSNEIVEIPYNAPDTVQDVINRINNSNAQ 412 ++ +++ + + K+ F +++ T A +T V N A Sbjct: 344 VAIGATVTDASAVLATDYKISFDNN-QWQVTRL---------ASNTTFTVTPDANGKVAF 393 Query: 413 VTARINSEGKLEIKAVKEQEDENITFKIKHIEDSGSFLTKYTGILNASGPEGAYDYKNID 472 + G AV + +F +K + D I+N ++ Sbjct: 394 DGLELTFTGTP---AVND------SFTLKPVSD---------AIVNM----------DVL 425 Query: 473 TTD--KLAPKSTYSISPLKNPAAWIKVADIIDSDPSKIASGIKNPTNEISIGDNQAALRI 530 TD K+A S + A DSD + + +N ++G ++ Sbjct: 426 ITDEAKIAMAS-------EEDAG--------DSDNRNGQALLDLQSNSKTVGGAKSF--N 468 Query: 531 SSFGNSQIMIGKNLTLNDYFANTASNIAIKGQISEITKESQSQILKDLTDLRMSISGVNK 590 ++ + IG + T N+ +++++ + Q SISGVN Sbjct: 469 DAYASLVSDIGNKTATLKTSSATQGNV-----VTQLSNQQQ------------SISGVNL 511 Query: 591 DEELANMIEFQQAFIAASKFITVSVELIDTVIN 623 DEE N+ FQQ ++A ++ + + + D +IN Sbjct: 512 DEEYGNLQRFQQYYLANAQVLQTANAIFDALIN 544
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
BBUZS7_RS02245	FLAGELLIN	57	8e-11	Flagellin signature.
>FLAGELLIN#Flagellin signature. Length = 507 Score = 56.6 bits (136), Expect = 8e-11 Identities = 37/133 (27%), Positives = 59/133 (44%), Gaps = 5/133 (3%) Query: 10 TYENFKTSSAEQESKITKLLENLYKGGKRIVKLRNDPTGVTHAIRLDNDIFKLNVYIKNI 69 T N S + S I +L G RI ++D G A R ++I L +N Sbjct: 13 TQNNLNKSQSSLSSAIERL-----SSGLRINSAKDDAAGQAIANRFTSNIKGLTQASRNA 67 Query: 70 DTSKSNLRYTEGYLRSLTNILTRAKEIAIQGASGTYESDDKKMISKEVNALLEDVVAIAN 129 + S + TEG L + N L R +E+++Q +GT D K I E+ LE++ ++N Sbjct: 68 NDGISIAQTTEGALNEINNNLQRVRELSVQATNGTNSDSDLKSIQDEIQQRLEEIDRVSN 127 Query: 130 AKGPDGYSIFSGT 142 +G + S Sbjct: 128 QTQFNGVKVLSQD 140 Score = 30.8 bits (69), Expect = 0.012 Identities = 33/358 (9%), Positives = 87/358 (24%), Gaps = 12/358 (3%) Query: 61 KLNVYIKNIDTSKSNLRYTEGYLRSLTNILTRAKEIAIQGASGTYESDDKKMISKEVNAL 120 + + ++ ID L + TY K + Sbjct: 154 TITIDLQKIDVKSLGLDGFNVNGPKEATVGDLKSSFKNVTGYDTYAVGANKYRVDVNSGA 213 Query: 121 LEDVVAIANAKGPDGYSIFSGTKIDSEAFKVTRENKISKTSKDGAGPQIIKVEYNGNQAE 180 + + +G +A T + T + + + Sbjct: 214 VVTDTTAPTVPDKVYVNAANGQLTTDDAENNTAVDLFKTTKSTAGTAEAKAIAGAIKGGK 273 Query: 181 KKTEVYNDIHMSNNYPGNVIFFLQNQNIISSINTNGFAVKENTKIYIDNIEIGLTAGDTA 240 + + + +S+ I + ++ Sbjct: 274 EGDTFDYK---GVTFTIDTKTGNDGNGKVSTTINGEKVTLTVADITAGAANVDAATLQSS 330 Query: 241 LDIVAKINESSAPVEASIDPVLNSLSIKTTTPHQIWITEEKESNVLQTLGILTKNNDTKL 300 ++ + + LS + + + K+ Sbjct: 331 KNVYTSVVNGQFTFDDKTKNESAKLSDLEANN----AVKGESKITVNGAEYTANAAGDKV 386 Query: 301 PPYNLSSSTEVRSRSIFDALIELRDTLYNNKEELVGSRSLAEIDESLKRLLISVADLGAK 360 + + + + + E + + S ID +L ++ + LGA Sbjct: 387 TLAGKTMFIDKTASGVSTLINEDAAAAKKSTANPLAS-----IDSALSKVDAVRSSLGAI 441 Query: 361 ENRLDRSYERISKEAADMKEDMIQYTDLDVTKAITNLNMASLAYQVSIGISAKIMQTT 418 +NR D + + ++ + D D ++N++ A + Q + A+ Q Sbjct: 442 QNRFDSAITNLGNTVTNLNSARSRIEDADYATEVSNMSKAQILQQAGTSVLAQANQVP 499

BBUZS7_RS02705

BBUZS7_RS02855

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
BBUZS7_RS02705	-1	18	1.326392	flagellar biosynthesis protein FlhF
BBUZS7_RS02710	-1	17	0.987367	flagellar biosynthesis protein FlhA
BBUZS7_RS02715	0	19	-0.127520	flagellar biosynthesis protein FlhB
BBUZS7_RS02720	-1	17	0.580219	flagellar biosynthetic protein FliR
BBUZS7_RS02725	0	17	1.696137	flagellar biosynthesis protein FliQ
BBUZS7_RS02730	0	16	2.269566	flagellar type III secretion system pore protein
BBUZS7_RS02735	1	17	3.126565	flagellar biosynthesis protein FliZ
BBUZS7_RS02740	1	18	4.130941	flagellar motor switch protein FliN
BBUZS7_RS02745	1	17	4.930465	flagellar motor switch protein FliM
BBUZS7_RS02750	0	19	4.530761	flagellar basal body-associated protein FliL
BBUZS7_RS02755	-2	20	2.587759	flagellar motor protein MotB
BBUZS7_RS02760	-2	20	1.994362	motility protein A
BBUZS7_RS02765	-2	16	0.460954	flagellar protein FlbD
BBUZS7_RS02770	-2	15	1.231390	flagellar hook protein FlgE
BBUZS7_RS02775	1	16	-0.264266	flagellar hook assembly protein FlgD
BBUZS7_RS02780	5	16	0.740001	flagellar hook-length control protein FliK
BBUZS7_RS02785	6	17	3.013096	flagellar protein
BBUZS7_RS02790	5	18	3.269873	flagellar protein FlbA
BBUZS7_RS02795	4	19	4.263700	flagellar protein export ATPase FliI
BBUZS7_RS02800	6	19	3.992607	flagellar assembly protein FliH
BBUZS7_RS02805	4	19	3.599347	flagellar motor switch protein FliG
BBUZS7_RS02810	2	18	3.486817	flagellar basal body M-ring protein FliF
BBUZS7_RS02815	1	19	2.204966	flagellar hook-basal body complex protein FliE
BBUZS7_RS02820	1	18	1.529780	flagellar basal body rod protein FlgC
BBUZS7_RS02825	1	19	2.794980	flagellar basal body rod protein FlgB
BBUZS7_RS02830	0	18	3.837032	HslU--HslV peptidase ATPase subunit
BBUZS7_RS02835	-1	19	3.278012	ATP-dependent protease subunit HslV
BBUZS7_RS02840	-2	17	2.479007	DNA-protecting protein DprA
BBUZS7_RS02845	-1	16	2.566078	hypothetical protein
BBUZS7_RS02850	-1	15	2.485274	cell division protein FtsZ
BBUZS7_RS02855	0	13	0.737662	cell division protein FtsA

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
BBUZS7_RS02705	PF05272	31	0.011	Virulence-associated E family protein
>PF05272#Virulence-associated E family protein Length = 892 Score = 30.8 bits (69), Expect = 0.011 Identities = 8/23 (34%), Positives = 12/23 (52%) Query: 176 VFILVGPTGVGKTTTIAKLAAIY 198 +L G G+GK+T I L + Sbjct: 598 SVVLEGTGGIGKSTLINTLVGLD 620
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
BBUZS7_RS02715	TYPE3IMSPROT	339	e-117	Type III secretion system inner membrane S protein ...
>TYPE3IMSPROT#Type III secretion system inner membrane S protein family signature. Length = 354 Score = 339 bits (871), Expect = e-117 Identities = 101/345 (29%), Positives = 182/345 (52%), Gaps = 9/345 (2%) Query: 25 RTELPTDQKKQKAREEGRVLKSTEINTAVSLLLLFALFFFMLSYFA---LDLIAVFKEQA 81 +TE PT +K + AR++G+V KS E+ + ++ L A+ + Y+ L+ + EQ+ Sbjct: 5 KTEQPTPKKIRDARKKGQVAKSKEVVSTALIVALSAMLMGLSDYYFEHFSKLMLIPAEQS 64 Query: 82 IKLPEVMRMSVYTMGFAYIRSIMGYVVLFFFASLAVNFFVNIIQVGFFITFKSLEPRWDK 141 LP +S + + +L A +A+ +++Q GF I+ ++++P K Sbjct: 65 Y-LPFSQALSYVVDNVLLEFFYLCFPLLTVAALMAIA--SHVVQYGFLISGEAIKPDIKK 121 Query: 142 ISFNFSRWAKNSFFSAGAFFNLFKSLLKVVIICLIYYFIIENNIGKISKLSEYTLQSGIS 201 I N AK FS + KS+LKVV++ ++ + II+ N+ + +L ++ Sbjct: 122 I--NPIEGAKR-IFSIKSLVEFLKSILKVVLLSILIWIIIKGNLVTLLQLPTCGIECITP 178 Query: 202 IVLVIAYKICFFSVMFLAIVGVFDYLFQRSQYIESLKMTKEEVKQERKEMEGDPLLRSRI 261 ++ I ++ + ++ + DY F+ QYI+ LKM+K+E+K+E KEMEG P ++S+ Sbjct: 179 LLGQILRQLMVICTVGFVVISIADYAFEYYQYIKELKMSKDEIKREYKEMEGSPEIKSKR 238 Query: 262 KERMRVILSTNLRVAIPQADVVITNPEHFAVAIKWDSETMLAPKVLAKGQDEIALTIKKI 321 ++ + I S N+R + ++ VV+ NP H A+ I + P V K D T++KI Sbjct: 239 RQFHQEIQSRNMRENVKRSSVVVANPTHIAIGILYKRGETPLPLVTFKYTDAQVQTVRKI 298 Query: 322 ARENNVPLMENKLLARALYANVKVNEEIPREYWEIVSKILVRVYS 366 A E VP+++ LARALY + V+ IP E E +++L + Sbjct: 299 AEEEGVPILQRIPLARALYWDALVDHYIPAEQIEATAEVLRWLER 343
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
BBUZS7_RS02720	TYPE3IMRPROT	113	2e-32	Type III secretion system inner membrane R protein ...
>TYPE3IMRPROT#Type III secretion system inner membrane R protein family signature. Length = 261 Score = 113 bits (285), Expect = 2e-32 Identities = 46/242 (19%), Positives = 107/242 (44%), Gaps = 4/242 (1%) Query: 16 VLVRIFMFLKFSPFFSTIKI-GYFNFFFSLILSVIVVEKIKIIYPLDNMLSFALILLGEA 74 L+R+ + +P S + +++++ + + + + + Sbjct: 19 PLLRVLALISTAPILSERSVPKRVKLGLAMMITFAIAPSLPANDVPVFSFFALWLAVQQI 78 Query: 75 ILGLIQAFFVNIIFNVFHLVGFFFSNQIGLAYANIFDVFSEEDSMIISQIFAYLFLLLFL 134 ++G+ F + F G Q+GL++A D S + ++++I L LLLFL Sbjct: 79 LIGIALGFTMQFAFAAVRTAGEIIGLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFL 138 Query: 135 SSDFLLRFFVIGIHDSVLNIRVEHLVNMRNSGFVKLLLMSFGFLFEKALLISFPILSLLL 194 + + L + + + D+ + + NS L + +F L+++ P+++LLL Sbjct: 139 TFNGHL-WLISLLVDTFHTLPI--GGEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLL 195 Query: 195 LFYLVLGILSKSSPQINLLIISFSTSLFLGLLILYIGFPSLAISSKRVIELSLDSLASFL 254 L LG+L++ +PQ+++ +I F +L +G+ ++ P +A + + + LA + Sbjct: 196 TLNLALGLLNRMAPQLSIFVIGFPLTLTVGISLMAALMPLIAPFCEHLFSEIFNLLADII 255 Query: 255 KL 256 Sbjct: 256 SE 257
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
BBUZS7_RS02725	TYPE3IMQPROT	61	2e-16	Type III secretion system inner membrane Q protein ...
>TYPE3IMQPROT#Type III secretion system inner membrane Q protein family signature. Length = 86 Score = 61.3 bits (149), Expect = 2e-16 Identities = 21/76 (27%), Positives = 43/76 (56%) Query: 6 ILYLIRISIENIIILSAPMLIIALIVGLLISIFQAITSIQDQTLSFIPKIIVILLVIVIF 65 +++ ++ ++ILS I+A I+GLL+ +FQ +T +Q+QTL F K++ + L + + Sbjct: 4 LVFAGNKALYLVLILSGWPTIVATIIGLLVGLFQTVTQLQEQTLPFGIKLLGVCLCLFLL 63 Query: 66 GPWILNKLMQFTYMIF 81 W L+ + + Sbjct: 64 SGWYGEVLLSYGRQVI 79
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
BBUZS7_RS02730	FLGBIOSNFLIP	260	3e-90	Escherichia coli: Flagellar biosynthetic protein Fl...
>FLGBIOSNFLIP#Escherichia coli: Flagellar biosynthetic protein FliP signature. Length = 245 Score = 260 bits (667), Expect = 3e-90 Identities = 97/213 (45%), Positives = 138/213 (64%), Gaps = 3/213 (1%) Query: 41 GGSEIAFSLQLLILLTIITLSPAFLVLMTSFLRISIVLDFIRRALSLQQSPPTQIVMGLA 100 GG + +Q L+ +T +T PA L++MTSF RI IV +R AL +PP Q+++GLA Sbjct: 34 GGQSWSLPVQTLVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLA 93 Query: 101 LFLTIFTMWPTFNSIYEQAYLPLKESKINFNEFYNKGIAPLRIFMYKQMSDGRHEEIRLF 160 LFLT F M P + IY AY P E KI+ E KG PLR FM +Q R ++ LF Sbjct: 94 LFLTFFIMSPVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQT---READLGLF 150 Query: 161 MSMSNYDRPKNFSEVPTHVLIAAFILHELKVAFKMGILIFLPFIVLDIIVASVLMAMGMI 220 ++N + VP +L+ A++ ELK AF++G IF+PF+++D+++ASVLMA+GM+ Sbjct: 151 ARLANTGPLQGPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMM 210 Query: 221 MLPPVMISLPFKLILFVMVDGWTLITSGLIKSF 253 M+PP I+LPFKL+LFV+VDGW L+ L +SF Sbjct: 211 MVPPATIALPFKLMLFVLVDGWQLLVGSLAQSF 243
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
BBUZS7_RS02740	FLGMOTORFLIN	103	7e-32	Flagellar motor switch protein FliN signature.
>FLGMOTORFLIN#Flagellar motor switch protein FliN signature. Length = 137 Score = 103 bits (258), Expect = 7e-32 Identities = 40/77 (51%), Positives = 62/77 (80%) Query: 35 NFGLLMDVSMQLTVELGRTERKIKDILGMSEGTIITLDKLAGEPVDILVNGKIVAKGEVV 94 + L+MD+ ++LTVELGRT IK++L +++G+++ LD LAGEP+DIL+NG ++A+GEVV Sbjct: 53 DIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILINGYLIAQGEVV 112 Query: 95 VIDENFGVRITEIIKTK 111 V+ + +GVRIT+II Sbjct: 113 VVADKYGVRITDIITPS 129
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
BBUZS7_RS02745	FLGMOTORFLIM	458	e-165	Flagellar motor switch protein FliM signature.
>FLGMOTORFLIM#Flagellar motor switch protein FliM signature. Length = 344 Score = 458 bits (1181), Expect = e-165 Identities = 195/345 (56%), Positives = 259/345 (75%), Gaps = 9/345 (2%) Query: 8 LSQDDIDSLLESINSSESLSLDESLSNVISSPTGKKQKVKVYDFKRPDKFSKEQVRTVSS 67 LSQD+ID LL +I+S ++ D + P +K+ +YDF+RPDKFSKEQ+RT+S Sbjct: 5 LSQDEIDQLLTAISSGDASIED-------ARPISDTRKITLYDFRRPDKFSKEQMRTLSL 57 Query: 68 FHEAFARYTTTSLSALLRKMVHVHVASVDQLTYEEFIRSIPNPTTLAIINMDPLKGSAIF 127 HE FAR TTTSLSA LR MVHVHVASVDQLTYEEFIRSIP P+TLA+I MDPLKG+A+ Sbjct: 58 MHETFARLTTTSLSAQLRSMVHVHVASVDQLTYEEFIRSIPTPSTLAVITMDPLKGNAVL 117 Query: 128 EVDPTIAFAIVDRLFGGDGDTIKDKSRDLTEIEQSVMESVIIRILANMREAWSQVVDLRP 187 EVDP+I F+I+DRLFGG G K + RDLT+IE SVME VI+RILAN+RE+W+QV+DLRP Sbjct: 118 EVDPSITFSIIDRLFGGTGQAAKVQ-RDLTDIENSVMEGVIVRILANVRESWTQVIDLRP 176 Query: 188 RFGHIEVNPQFAQIVPPTEMIILVTLEVKIGKVEGLMNFCLPYITIEPIVSKLSTRYWHS 247 R G IE NPQFAQIVPP+EM++LVTLE K+G+ EG+MNFC+PYITIEPI+SKLS+++W S Sbjct: 177 RLGQIETNPQFAQIVPPSEMVVLVTLETKVGEEEGMMNFCIPYITIEPIISKLSSQFWFS 236 Query: 248 LIGVGTTSENLDALREKLENTAMPLVAEIGEVKLKVREILSLDKGDVLNLESSLINKDLT 307 + +T++ + LR+KL M +VAE+G ++L VR+IL L GD++ L + + Sbjct: 237 SVRRSSTTQYMGVLRDKLSTVDMDVVAEVGSLRLSVRDILGLRVGDIIRLHDTHVGDPFV 296 Query: 308 LKVGTKEKFKCRMGLMGNKVSVQITEKIGDIKGFDLLKELTEEVE 352 L +G ++KF C+ G++G K++ QI E+I D +EL+ + E Sbjct: 297 LSIGNRKKFLCQPGVVGKKIAAQILERIESTSQED-FEELSADEE 340
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
BBUZS7_RS02755	OMPADOMAIN	55	8e-11	OMPA domain signature.
>OMPADOMAIN#OMPA domain signature. Length = 346 Score = 54.6 bits (131), Expect = 8e-11 Identities = 28/132 (21%), Positives = 57/132 (43%), Gaps = 21/132 (15%) Query: 124 ISLAADAFFDSASADVKLEENRDSIQKIASFIGFLSPRGYNFKIEGHTDNIDTDVNGPWK 183 +L +D F+ A +K E + ++ ++ S + L P+ + + G+TD I +D Sbjct: 215 FTLKSDVLFNFNKATLK-PEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSD-----A 268 Query: 184 SNWELSAARSVNMLEHILNYLDQSDVKRIENNFEVSGFGGSRPIATDDT---------PE 234 N LS R+ + +++YL + + G G S P+ + + Sbjct: 269 YNQGLSERRA----QSVVDYLISKGIPA--DKISARGMGESNPVTGNTCDNVKQRAALID 322 Query: 235 GRAYNRRIDILI 246 A +RR++I + Sbjct: 323 CLAPDRRVEIEV 334
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
BBUZS7_RS02770	FLGHOOKAP1	51	4e-09	Flagellar hook-associated protein signature.
>FLGHOOKAP1#Flagellar hook-associated protein signature. Length = 546 Score = 51.5 bits (123), Expect = 4e-09 Identities = 18/89 (20%), Positives = 33/89 (37%), Gaps = 13/89 (14%) Query: 4 SLYSGVSGLQNHQTRMDVVGNNIANVNTIGFKKGRVNFQDMISQSISGASRPTDARGGTN 63 + + +SGL Q ++ NNI++ N G+ + I + T GG Sbjct: 3 LINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTT---------IMAQANSTLGAGGW- 52 Query: 64 PKQVGLGMNVASIDTIHTQGAFQSTQKAS 92 VG G+ V+ + + + A Sbjct: 53 ---VGNGVYVSGVQREYDAFITNQLRAAQ 78 Score = 40.7 bits (95), Expect = 1e-05 Identities = 9/48 (18%), Positives = 28/48 (58%) Query: 394 IRSGVLEMANVDLAEQFTDMIVTQRGFQANAKTITTSDQLLQELVRLK 441 + + ++ V+L E++ ++ Q+ + ANA+ + T++ + L+ ++ Sbjct: 499 LSNQQQSISGVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR 546
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
BBUZS7_RS02780	FLGHOOKFLIK	39	2e-05	Flagellar hook-length control protein signature.
>FLGHOOKFLIK#Flagellar hook-length control protein signature. Length = 375 Score = 39.4 bits (91), Expect = 2e-05 Identities = 24/82 (29%), Positives = 45/82 (54%), Gaps = 5/82 (6%) Query: 281 KLVLKPKELGSIRINLNLDSNNNLLGKIVVDNQNVKMLFDQNMHSLNKMLGESGFNASLN 340 +L L P++LG ++I+L +D N + ++V +Q+V+ + + L L ESG + Sbjct: 260 ELRLHPQDLGEVQISLKVDDNQAQI-QMVSPHQHVRAALEAALPVLRTQLAESGIQLGQS 318 Query: 341 LFLAGENLNSFTGDFKDDSKDQ 362 ++GE SF+G + S+ Q Sbjct: 319 -NISGE---SFSGQQQAASQQQ 336
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
BBUZS7_RS02785	TYPE4SSCAGX	29	0.019	Type IV secretion system CagX conjugation protein si...
>TYPE4SSCAGX#Type IV secretion system CagX conjugation protein signature. Length = 522 Score = 28.6 bits (63), Expect = 0.019 Identities = 34/143 (23%), Positives = 66/143 (46%), Gaps = 20/143 (13%) Query: 39 RYFPEFVRTKLLGETSLVFDHNSNIILDEAR--LVKEREAIDIKNQQIEKLKEDLKLKED 96 R + EF++TK L+ D L+E + L KE+EA + + Q+ +K K + + +E Sbjct: 120 RDYQEFLKTK-----KLIVDAPDPKELEEQKKALEKEKEAKE-QAQKAQKDKREKRKEER 173 Query: 97 SLNKLEFELKQKQKDLDLKQKVIDDIINKYNDEEANILQTAVYLMNMPPEDAVKRLEDLN 156 + N+ E + + + N N L + D ++RLED+ Sbjct: 174 AKNRANLE------------NLTNAMSNPQNLSNNKNLSELIKQQRENELDQMERLEDMQ 221 Query: 157 PELAISYMRKIEELSKKEGRLSI 179 + + +++IEEL+KK+ ++ Sbjct: 222 EQAQANALKQIEELNKKQAEEAV 244
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
BBUZS7_RS02800	FLGFLIH	47	3e-08	Flagellar assembly protein FliH signature.
>FLGFLIH#Flagellar assembly protein FliH signature. Length = 228 Score = 46.7 bits (110), Expect = 3e-08 Identities = 43/197 (21%), Positives = 96/197 (48%), Gaps = 22/197 (11%) Query: 115 KESIETESNAEIER-LAREYEEKLKTDLDIAIAKGREEGYSKGY--------ESGFEDFD 165 +E+I E+ +E+ LA+ + + IA+GR++G+ +GY E G + Sbjct: 29 EETIIEEAEPSLEQQLAQLQMQAHEQGYQAGIAEGRQQGHKQGYQEGLAQGLEQGLAEAK 88 Query: 166 KVMRKLHAIIASLIAERKGILESSSGQIVSLVMQIAIKVIKRITDSQKDI----VLENVN 221 +HA + L++E + L++ I S +MQ+A++ +++ + +++ + Sbjct: 89 SQQAPIHARMQQLVSEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQ 148 Query: 222 EVLKR---VKDKTQITIRVNLDDLDIVRHKKSDFISRFDIIENLEIIEDPNIGKGGCIIE 278 ++L++ K Q +RV+ DDL V +S + + DP + GGC + Sbjct: 149 QLLQQEPLFSGKPQ--LRVHPDDLQRVDDMLGATLS----LHGWRLRGDPTLHPGGCKVS 202 Query: 279 TNFGEIDARISSQLDKI 295 + G++DA ++++ ++ Sbjct: 203 ADEGDLDASVATRWQEL 219
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
BBUZS7_RS02805	FLGMOTORFLIG	427	e-153	Flagellar motor switch protein FliG signature.
>FLGMOTORFLIG#Flagellar motor switch protein FliG signature. Length = 344 Score = 427 bits (1100), Expect = e-153 Identities = 344/344 (100%), Positives = 344/344 (100%) Query: 1 MEEKKEKEILDVSALTGKQKAAILLVSIGSEISSKVFKYLSQEEIESLTFEIAKLETITS 60 MEEKKEKEILDVSALTGKQKAAILLVSIGSEISSKVFKYLSQEEIESLTFEIAKLETITS Sbjct: 1 MEEKKEKEILDVSALTGKQKAAILLVSIGSEISSKVFKYLSQEEIESLTFEIAKLETITS 60 Query: 61 ELKDNVLLEFKELMMAQEFIQKGGIDYARELLEKSLGTQKAVDIINNLGSALQSRPFEFV 120 ELKDNVLLEFKELMMAQEFIQKGGIDYARELLEKSLGTQKAVDIINNLGSALQSRPFEFV Sbjct: 61 ELKDNVLLEFKELMMAQEFIQKGGIDYARELLEKSLGTQKAVDIINNLGSALQSRPFEFV 120 Query: 121 RRADPANILNFIQQEHPQTIALILSYLDPQKASFILSSLPTEVQTNVARRIALMDRTSPE 180 RRADPANILNFIQQEHPQTIALILSYLDPQKASFILSSLPTEVQTNVARRIALMDRTSPE Sbjct: 121 RRADPANILNFIQQEHPQTIALILSYLDPQKASFILSSLPTEVQTNVARRIALMDRTSPE 180 Query: 181 VVREVERVLEKKLASLSSEDYTSAGGVDNVVEIINMADRKTEKFIIESLEEEDPELAEEI 240 VVREVERVLEKKLASLSSEDYTSAGGVDNVVEIINMADRKTEKFIIESLEEEDPELAEEI Sbjct: 181 VVREVERVLEKKLASLSSEDYTSAGGVDNVVEIINMADRKTEKFIIESLEEEDPELAEEI 240 Query: 241 KKKMFVFEDIVLLDDRSIQRVLREIDGQELAKALKSVDIPVQEKIFKNMSKRAASMLKED 300 KKKMFVFEDIVLLDDRSIQRVLREIDGQELAKALKSVDIPVQEKIFKNMSKRAASMLKED Sbjct: 241 KKKMFVFEDIVLLDDRSIQRVLREIDGQELAKALKSVDIPVQEKIFKNMSKRAASMLKED 300 Query: 301 MEFLGPTRRKDVEESQQKIVSLIRKLEEQGEIVISRGGEEDVLV 344 MEFLGPTRRKDVEESQQKIVSLIRKLEEQGEIVISRGGEEDVLV Sbjct: 301 MEFLGPTRRKDVEESQQKIVSLIRKLEEQGEIVISRGGEEDVLV 344
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
BBUZS7_RS02810	FLGMRINGFLIF	162	1e-45	Flagellar M-ring protein signature.
>FLGMRINGFLIF#Flagellar M-ring protein signature. Length = 559 Score = 162 bits (412), Expect = 1e-45 Identities = 112/565 (19%), Positives = 222/565 (39%), Gaps = 55/565 (9%) Query: 23 QKIALGLIIFFVILALVFLIGFSTKSQSIALF-GVEIKDQYLLDRISQRLDRENVKYFLS 81 +I L + + +V ++ ++ LF + +D I +L + N+ Y + Sbjct: 23 PRIPLIVAGSAAVAIVVAMVLWAKTPDYRTLFSNLSDQD---GGAIVAQLTQMNIPYRFA 79 Query: 82 SDGRIYLDDEKLAKKMRAILVREELVPVHMDPWALFDIDRWTITDFERSINLRRSITRAV 141 + ++R L ++ L + L D +++ I+ F +N +R++ + Sbjct: 80 NGSGAIEVPADKVHELRLRLAQQGLPKGGAVGFELLDQEKFGISQFSEQVNYQRALEGEL 139 Query: 142 EQHIVALDDVDAVSVNLVMPEKALFKESQEPVKASVRITPRPGSDIITNRKKVEGLVKLI 201 + I L V + V+L MP+ +LF Q+ ASV +T PG + + ++ +V L+ Sbjct: 140 ARTIETLGPVKSARVHLAMPKPSLFVREQKSPSASVTVTLEPGRAL--DEGQISAVVHLV 197 Query: 202 QYAIEGLESDNIAIVDNSGTILNDFSNLDGIDRIDLAEKERKLKLKYEAMLRGEIDSALS 261 A+ GL N+ +VD SG +L SN G DL + + K E+ ++ I++ LS Sbjct: 198 SSAVAGLPPGNVTLVDQSGHLL-TQSNTSG---RDLNDAQLKFANDVESRIQRRIEAILS 253 Query: 262 KVLSVDRFMIARVNVKLDTSKETTESKEYAPIELQSQDPKASYNTRKVSDSTIISSQTQK 321 ++ A+V +LD + + + Y+P KA+ +R+++ S + + Sbjct: 254 PIVGNGNV-HAQVTAQLDFANKEQTEEHYSP---NGDASKATLRSRQLNISEQVGAGYPG 309 Query: 322 KEYQGQGYSPWGPPGQEGNTPPEYQDLSD-------------ITGKYNESQEIKNVALNE 368 P P TPP Q + + + E N ++ Sbjct: 310 GVPGALSNQPAPPNEAPIATPPTNQQNAQNTPQTSTSTNSNSAGPRSTQRNETSNYEVDR 369 Query: 369 KKSTSEKEPARIVGVSLGIFVDGIWNFVYDEKGDFVIENGMRKREYKPMALEEIKNIEDV 428 ++ I +S+ + V+ + + P+ +++K IED+ Sbjct: 370 TIRHTKMNVGDIERLSVAVVVNY---------------KTLADGKPLPLTADQMKQIEDL 414 Query: 429 LQSSFEYKPERGDSITVRNISFDRMNEFREIDENYFASER--FKYFLFIASIVFSLLILV 486 + + + +RGD++ V N F ++ E F ++ L + L++ Sbjct: 415 TREAMGFSDKRGDTLNVVNSPFSAVDN--TGGELPFWQQQSFIDQLLAAGRWLLVLVVAW 472 Query: 487 FTIFFAISRELERRRRLREEELAKQAHLRRQQALMDG-----GDDIGVDDVVGGIREGDE 541 A+ +L RR EE A Q + +Q + D + R G E Sbjct: 473 ILWRKAVRPQLTRR---VEEAKAAQEQAQVRQETEEAVEVRLSKDEQLQQRRANQRLGAE 529 Query: 542 LQSNA-ELLAREKPEDVAKLIRTWL 565 + S ++ P VA +IR W+ Sbjct: 530 VMSQRIREMSDNDPRVVALVIRQWM 554
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
BBUZS7_RS02815	FLGHOOKFLIE	86	2e-25	Flagellar hook-basal body complex protein FliE signa...
>FLGHOOKFLIE#Flagellar hook-basal body complex protein FliE signature. Length = 103 Score = 85.9 bits (212), Expect = 2e-25 Identities = 15/71 (21%), Positives = 38/71 (53%) Query: 40 TFKDVLINSITDVNKSQLNVSKVTEQAILKPSSIDVHDVVIAMSKANMNLSILKAVVERG 99 +F L ++ ++ +Q E+ L + ++DV+ M KA++++ + V + Sbjct: 32 SFAGQLHAALDRISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKL 91 Query: 100 VKAYQDIINIR 110 V AYQ++++++ Sbjct: 92 VAAYQEVMSMQ 102
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
BBUZS7_RS02820	FLGHOOKAP1	48	3e-09	Flagellar hook-associated protein signature.
>FLGHOOKAP1#Flagellar hook-associated protein signature. Length = 546 Score = 47.6 bits (113), Expect = 3e-09 Identities = 21/80 (26%), Positives = 35/80 (43%), Gaps = 15/80 (18%) Query: 5 SSINVASTGLTAQRLRIDVISNNIANVSTSRTPDGGPYRRQRIIFAPRVNNPYWKGPFIP 64 S IN A +GL A + ++ SNNI++ + + Y RQ I A N Sbjct: 2 SLINNAMSGLNAAQAALNTASNNISSYNVA------GYTRQTTIMAQ--ANSTLGA---- 49 Query: 65 DYLDNGIGQGVRVASIEKDK 84 +G GV V+ ++++ Sbjct: 50 ---GGWVGNGVYVSGVQREY 66 Score = 44.2 bits (104), Expect = 5e-08 Identities = 11/46 (23%), Positives = 23/46 (50%) Query: 104 KKGYVELPNVNLVEEMVDMISASRAYEANSTVINSSKSMFRSALAI 149 + VNL EE ++ + Y AN+ V+ ++ ++F + + I Sbjct: 500 SNQQQSISGVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINI 545
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
BBUZS7_RS02830	HTHFIS	31	0.010	FIS bacterial regulatory protein HTH signature.
>HTHFIS#FIS bacterial regulatory protein HTH signature. Length = 484 Score = 31.0 bits (70), Expect = 0.010 Identities = 13/70 (18%), Positives = 30/70 (42%), Gaps = 15/70 (21%) Query: 20 KYIIGQDEAKKLVSIALVNRYIRSRLPKEIKDEVMPKNIIMIGSTGIGKTEIAR---RLS 76 ++G+ A + + ++ R +++ L +++ G +G GK +AR Sbjct: 137 MPLVGRSAAMQEI-YRVLARLMQTDLT-----------LMITGESGTGKELVARALHDYG 184 Query: 77 KLIKAPFIKV 86 K PF+ + Sbjct: 185 KRRNGPFVAI 194
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
BBUZS7_RS02845	SYCDCHAPRONE	29	0.012	Gram-negative bacterial type III secretion SycD cha...
>SYCDCHAPRONE#Gram-negative bacterial type III secretion SycD chaperone signature. Length = 168 Score = 28.7 bits (64), Expect = 0.012 Identities = 16/91 (17%), Positives = 34/91 (37%), Gaps = 2/91 (2%) Query: 69 ARFFNLIGLEFFKLGQYGPAIEYFAKNLEINPNNYLSHFYIGVASYNLAKNLRVKDEVEK 128 +RFF +G +GQY AI ++ ++ F+ + + + Sbjct: 70 SRFFLGLGACRQAMGQYDLAIHSYSYGAIMDIKEPRFPFHAAECLLQKGELAEAESGLFL 129 Query: 129 YI-ILAENSFLKSLSIR-DDFKDSLFAISNM 157 ++A+ + K LS R +++ M Sbjct: 130 AQELIADKTEFKELSTRVSSMLEAIKLKKEM 160
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
BBUZS7_RS02855	SHAPEPROTEIN	56	8e-11	Bacterial cell shape determinant MreB/Mbl protein s...
>SHAPEPROTEIN#Bacterial cell shape determinant MreB/Mbl protein signature. Length = 347 Score = 55.9 bits (135), Expect = 8e-11 Identities = 50/226 (22%), Positives = 87/226 (38%), Gaps = 24/226 (10%) Query: 160 TGSSSSSQNLVR-CVNRAGFAVDEVVLGSLASSYATLSKEEREMGVLFIDMGKGTTDIIL 218 G++ + +R AG ++ +A++ G + +D+G GTT++ + Sbjct: 116 VGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATGSMVVDIGGGTTEVAV 175 Query: 219 YIDGSPYYTGVIPIGVNRVTLDIAQVWK------VPEDVAENIKITAGIAHPSILESQME 272 Y+ + IG +R I + + E AE IK G A+P ++E Sbjct: 176 ISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERIKHEIGSAYPGDEVREIE 235 Query: 273 TVIIPNLGTRPPQ--EKSRKELSVIINSRLREIFEMMKAEI------LKRGLYNKINGGI 324 V NL P+ + E+ + L I + + L + + G+ Sbjct: 236 -VRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALEQCPPELASDISER---GM 291 Query: 325 VLTGGGALFPGISNLIEEVFNYPARIGL-PMSINGIGE----EHID 365 VLTGGGAL + L+ E P + P++ G E ID Sbjct: 292 VLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALEMID 337

BBUZS7_RS03225

BBUZS7_RS03280

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
BBUZS7_RS03225	-1	11	0.914342	S-ribosylhomocysteine lyase
BBUZS7_RS03230	-1	15	1.431533	hypothetical protein
BBUZS7_RS03235	0	18	1.815651	HIT family protein
BBUZS7_RS03240	0	17	2.282228	magnesium transporter
BBUZS7_RS03245	0	17	2.451014	hypothetical protein
BBUZS7_RS03250	-1	18	3.723715	BMP family protein
BBUZS7_RS03255	0	20	4.098516	BMP family protein
BBUZS7_RS03260	1	20	4.458934	BMP family protein
BBUZS7_RS03265	1	19	5.024038	BMP family protein
BBUZS7_RS03270	2	19	4.957262	30S ribosomal protein S7
BBUZS7_RS03275	1	19	4.779428	30S ribosomal protein S12
BBUZS7_RS03280	0	19	4.756924	DNA-directed RNA polymerase subunit beta'

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
BBUZS7_RS03225	LUXSPROTEIN	188	3e-64	Bacterial autoinducer-2 (AI-2) production protein Lu...
>LUXSPROTEIN#Bacterial autoinducer-2 (AI-2) production protein LuxS signature. Length = 171 Score = 188 bits (479), Expect = 3e-64 Identities = 52/162 (32%), Positives = 86/162 (53%), Gaps = 11/162 (6%) Query: 4 ITSFTIDHTKLN-PGIYVSR-KDTFENVIFTTIDIRIKAPNIEPIIENAAIHTIEHIGAT 61 + SFT+DHT++N P + V++ T + T D+R APN + I+ IHT+EH+ A Sbjct: 3 LDSFTVDHTRMNAPAVRVAKTMQTPKGDTITVFDLRFTAPN-KDILSEKGIHTLEHLYAG 61 Query: 62 LLRNN-EVWTEKIVYFGPMGCRTGFYLIIFGDYESKDLVDLVSWLFSE----IVNFSEPI 116 +RN+ + +I+ PMGCRTGFY+ + G + + D +W+ + V I Sbjct: 62 FMRNHLNGDSVEIIDISPMGCRTGFYMSLIGTPSEQQVAD--AWIAAMEDVLKVENQNKI 119 Query: 117 PGASDKECGNYKEHNLDMAKYESSKYLQI-LNNIKEENLKYP 157 P ++ +CG H+LD AK + L++ + K + L P Sbjct: 120 PELNEYQCGTAAMHSLDEAKQIAKNILEVGVAVNKNDELALP 161
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
BBUZS7_RS03250	LIPPROTEIN48	73	2e-16	Mycoplasma P48 major surface lipoprotein signature.
>LIPPROTEIN48#Mycoplasma P48 major surface lipoprotein signature. Length = 428 Score = 72.7 bits (178), Expect = 2e-16 Identities = 72/286 (25%), Positives = 111/286 (38%), Gaps = 34/286 (11%) Query: 13 TSCFSRNGIESSS-KKIKISMLVD-GVLDDKSFNASANEALLRLKKDFPENIEEVFSCAI 70 T+ + ++++ K+K ++ D G +DDKSFN SA EAL + K IE Sbjct: 46 TNANGKQVVKNAELLKLKPVLITDEGKIDDKSFNQSAFEALKAINKQ--TGIEINNVEPS 103 Query: 71 SGVYSSYVSDLDNLKRNGSDLIWLVGYMLTDASL--LVSSENPKISYGIIDPIYGDDVQI 128 S S+Y S L + IW++ S+ + + ++ I I G D I Sbjct: 104 SNFESAYNSALSAGHK-----IWVLNGFKHQQSIKQYIDAHREELERNQIK-IIGIDFDI 157 Query: 129 PEN---LIAVVFRVEQGAFLAGYIAAKKSFSGK------IGFIGGMKGNIVDAFRYGYES 179 ++ F +++ AF GY A S + + GG V F G+ Sbjct: 158 ETEYKWFYSLQFNIKESAFTTGY-AIASWLSEQDESKRVVASFGGGAFPGVTTFNEGFAK 216 Query: 180 GAKYANKDIEIISEYSNSFSDVDIGRT-----------IASKMYSKGIDVIHFAAGLAGI 228 G Y N+ + Y S +D G T + S + H +AG Sbjct: 217 GILYYNQKHKSSKIYHTSPVKLDSGFTAGEKMNTVINNVLSSTPADVKYNPHVILSVAGP 276 Query: 229 GVIEAAKNLGDGYYVIGADQDQSY-LAPKNFITSVIKNIGDALYLI 273 E + G YVIG D DQ +TSV+K+I A+Y Sbjct: 277 ATFETVRLANKGQYVIGVDSDQGMIQDKDRILTSVLKHIKQAVYET 322
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
BBUZS7_RS03255	LIPPROTEIN48	70	3e-15	Mycoplasma P48 major surface lipoprotein signature.
>LIPPROTEIN48#Mycoplasma P48 major surface lipoprotein signature. Length = 428 Score = 69.7 bits (170), Expect = 3e-15 Identities = 69/261 (26%), Positives = 105/261 (40%), Gaps = 29/261 (11%) Query: 30 KVSLIID-GTFDDKSFNESALNGVKKVKEEFKIELVLKESSSNSYLSDLEGLKDAGSDLI 88 K LI D G DDKSFN+SA +K + ++ IE+ E SSN + S AG + Sbjct: 63 KPVLITDEGKIDDKSFNQSAFEALKAINKQTGIEINNVEPSSN-FESAYNSALSAGHKIW 121 Query: 89 WLIGYRFS------DMAKVAALQNPDMKYAIID-PIYSNDPIPANLVGMTFRAQEGAFLT 141 L G++ A L+ +K ID I + + F +E AF T Sbjct: 122 VLNGFKHQQSIKQYIDAHREELERNQIKIIGIDFDIETEYKW---FYSLQFNIKESAFTT 178 Query: 142 GY-IAARLSKTGK----IGFLGGIEGEIVDAFRYGYEAGAKYANKD-----------IKI 185 GY IA+ LS+ + + GG V F G+ G Y N+ +K+ Sbjct: 179 GYAIASWLSEQDESKRVVASFGGGAFPGVTTFNEGFAKGILYYNQKHKSSKIYHTSPVKL 238 Query: 186 STQYIGSFADLEAGRSVATRMYSDEIDIIHHAAGLGGIGAIEVAKELGSGHYIIGVDEDQ 245 + + +V + +D H + G E + G Y+IGVD DQ Sbjct: 239 DSGFTAGEKMNTVINNVLSSTPADVKYNPHVILSVAGPATFETVRLANKGQYVIGVDSDQ 298 Query: 246 AY-LAPDNVITSTTKDVGRAL 265 D ++TS K + +A+ Sbjct: 299 GMIQDKDRILTSVLKHIKQAV 319
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
BBUZS7_RS03260	LIPPROTEIN48	50	5e-09	Mycoplasma P48 major surface lipoprotein signature.
>LIPPROTEIN48#Mycoplasma P48 major surface lipoprotein signature. Length = 428 Score = 50.4 bits (120), Expect = 5e-09 Identities = 59/296 (19%), Positives = 103/296 (34%), Gaps = 50/296 (16%) Query: 18 FKSNKKSIKSDKV----VVGVLAHGSFYDKGYNQSVHDGVVKLRDNFGIKLITKSLRPYP 73 + K+ +K+ ++ V + G DK +NQS + + + GI++ Sbjct: 47 NANGKQVVKNAELLKLKPVLITDEGKIDDKSFNQSAFEALKAINKQTGIEINNVE----- 101 Query: 74 IEGKRLLTVDEAMTEDAYEVQKNPLNLFW-LIGYRFSDLSVKL------SYERPDIYYGI 126 + E AY + + W L G++ + ER I Sbjct: 102 ---------PSSNFESAYNSALSAGHKIWVLNGFKHQQSIKQYIDAHREELERNQI---K 149 Query: 127 IDAFDYGDIQVPKNSLAIKFRNEEAAFLAGYIAA-----KMSRKEKIGFLTGPMSEHVKD 181 I D+ K +++F +E+AF GY A + K + G V Sbjct: 150 IIGIDFDIETEYKWFYSLQFNIKESAFTTGYAIASWLSEQDESKRVVASFGGGAFPGVTT 209 Query: 182 FKFGFKAGIFYAN---PKLRLVSK---KAPSLFDKEKGKAMAL-------FMYKEDKVGV 228 F GF GI Y N ++ K S F + + + V Sbjct: 210 FNEGFAKGILYYNQKHKSSKIYHTSPVKLDSGFTAGEKMNTVINNVLSSTPADVKYNPHV 269 Query: 229 IFPIAGITGLGVYDAAKELGPKYYVIGLNQDQSYI-APQNVITSIIKDIGKVIYSI 283 I +AG ++ + YVIG++ DQ I ++TS++K I + +Y Sbjct: 270 ILSVAGPA---TFETVRLANKGQYVIGVDSDQGMIQDKDRILTSVLKHIKQAVYET 322
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
BBUZS7_RS03265	LIPPROTEIN48	60	3e-12	Mycoplasma P48 major surface lipoprotein signature.
>LIPPROTEIN48#Mycoplasma P48 major surface lipoprotein signature. Length = 428 Score = 60.0 bits (145), Expect = 3e-12 Identities = 55/272 (20%), Positives = 97/272 (35%), Gaps = 31/272 (11%) Query: 28 KTVSLIVDGAFDDKGFNESSSKAIRKLKADLNINIIEKASTGNSYLGDIANLEDGNSNLI 87 K V + +G DDK FN+S+ +A++ + I I +++ + + Sbjct: 63 KPVLITDEGKIDDKSFNQSAFEALKAINKQTGIE-INNVEPSSNFESAYNSALSAGHKIW 121 Query: 88 WGIGFRLSDILFQ---RASENVSVNYAIIEGV-YDEIQIPKNLLNISFRSEEVAFLAGY- 142 GF+ + Q E + N I G+ +D K ++ F +E AF GY Sbjct: 122 VLNGFKHQQSIKQYIDAHREELERNQIKIIGIDFDIETEYKWFYSLQFNIKESAFTTGYA 181 Query: 143 ----FASKASKTGKIGFVGGVRGKVLESFMYGYEAGAKYANSNIKVVSQYVGTFGDFGLG 198 + + + GG + +F G+ G Y N K Y + G Sbjct: 182 IASWLSEQDESKRVVASFGGGAFPGVTTFNEGFAKGILYYNQKHKSSKIYHTSPVKLDSG 241 Query: 199 ---------------RSTASNMYRDGVDIIFAAAGLSGIGVIEAAKELGPDHYIIGVDQD 243 ST +++ + I+ A + E + Y+IGVD D Sbjct: 242 FTAGEKMNTVINNVLSSTPADVKYNPHVILSVAGPATF----ETVRLANKGQYVIGVDSD 297 Query: 244 QSY-LAPNNVIVSAVKKVDSLMYS-LTKKYLE 273 Q + ++ S +K + +Y L LE Sbjct: 298 QGMIQDKDRILTSVLKHIKQAVYETLLDLILE 329
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
BBUZS7_RS03280	RTXTOXIND	31	0.037	Gram-negative bacterial RTX secretion protein D signat...
>RTXTOXIND#Gram-negative bacterial RTX secretion protein D signature. Length = 478 Score = 31.0 bits (70), Expect = 0.037 Identities = 7/17 (41%), Positives = 14/17 (82%) Query: 1192 KHLLVRDGDVVKAGDML 1208 K ++V++G+ V+ GD+L Sbjct: 108 KEIIVKEGESVRKGDVL 124

BBUZS7_RS05255

BBUZS7_RS05275

Pathogenicity Island (unbiased-composition)

LocusTag	DNBias	CDNBias	%GCBias	Product
BBUZS7_RS05255	1	22	0.612882	muramidase
BBUZS7_RS05260	2	17	1.254344	flagellar P-ring protein
BBUZS7_RS05265	1	13	2.469658	hypothetical protein
BBUZS7_RS05270	0	12	3.349793	flagellar basal-body rod protein FlgG
BBUZS7_RS05275	3	15	2.697866	flagellar basal-body rod protein FlgF

ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
BBUZS7_RS05255	FLGFLGJ	48	6e-10	Flagellar protein FlgJ signature.
>FLGFLGJ#Flagellar protein FlgJ signature. Length = 313 Score = 48.2 bits (114), Expect = 6e-10 Identities = 21/69 (30%), Positives = 39/69 (56%), Gaps = 2/69 (2%) Query: 37 DLRKASLEFEAMFIKQMLESMKKTLNKDQNLLNGGQVEEIFEDMLCEQRAKQMAQAQSFG 96 ++R + + E MF++ ML+SM+ L KD L + ++ M +Q A+QM + G Sbjct: 32 NIRPVARQVEGMFVQMMLKSMRDALPKDG--LFSSEHTRLYTSMYDQQIAQQMTAGKGLG 89 Query: 97 LADLIYNQL 105 LA+++ Q+ Sbjct: 90 LAEMMVKQM 98
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
BBUZS7_RS05260	FLGPRINGFLGI	257	7e-86	Flagellar P-ring protein signature.
>FLGPRINGFLGI#Flagellar P-ring protein signature. Length = 373 Score = 257 bits (658), Expect = 7e-86 Identities = 83/352 (23%), Positives = 153/352 (43%), Gaps = 60/352 (17%) Query: 35 SLSESVKLKEIADIYPTNTNFLTGIGIVAGLAGKGDSIKQKDL----IIKILEENNIINE 90 + +++ ++K+IA + N L G G+V GL G GDS++ + +L+ I + Sbjct: 24 AQADTSRIKDIASLQAGRDNQLIGYGLVVGLQGTGDSLRSSPFTEQSMRAMLQNLGITTQ 83 Query: 91 IGSNNIESKNIALVNVSLQVKGNTIKGSKHKACVASILDSKDLTNGILLKTNLKNKEGEI 150 G +N +KNIA V V+ + GS+ V+S+ D+ L G L+ T+L +G+I Sbjct: 84 GGQSN--AKNIAAVMVTANLPPFASPGSRVDVTVSSLGDATSLRGGNLIMTSLSGADGQI 141 Query: 151 IAIASGITQPNN-KLKGSGYTI-----------DSVIINEN--QNINHSYNIILKKGN-- 194 A+A G N +G T+ + II S N++L+ N Sbjct: 142 YAVAQGALIVNGFSAQGDAATLTQGVTTSARVPNGAIIERELPSKFKDSVNLVLQLRNPD 201 Query: 195 YTLINRIHKILTS---KKINNKI---KSDSTIEIEAKNIS----LLEEIENIKIETN--P 242 ++ R+ ++ + + + I + I ++ ++ L+ EIEN+ +ET+ Sbjct: 202 FSTAVRVADVVNAFARARYGDPIAEPRDSQEIAVQKPRVADLTRLMAEIENLTVETDTPA 261 Query: 243 KILIDKKNGIILASENAKI-------GTFTFSIEKDNQNI----FLSKNNKTTIQVNSMK 291 K++I+++ G I+ + +I GT T + + Q I F Q + M Sbjct: 262 KVVINERTGTIVIGADVRISRVAVSYGTLTVQVTESPQVIQPAPFSRGQTAVQPQTDIMA 321 Query: 292 LNE----FILK-----------NSNNLSNKELIQIIQAAQKINKLNGELILE 328 + E I++ NS L +I I+Q + L EL+L+ Sbjct: 322 MQEGSKVAIVEGPDLRTLVAGLNSIGLKADGIIAILQGIKSAGALQAELVLQ 373
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
BBUZS7_RS05270	FLGHOOKAP1	45	2e-07	Flagellar hook-associated protein signature.
>FLGHOOKAP1#Flagellar hook-associated protein signature. Length = 546 Score = 44.9 bits (106), Expect = 2e-07 Identities = 10/44 (22%), Positives = 23/44 (52%) Query: 220 ILEMSNVSIAEEMVTMIVAQRAYEINSKAIQTSDNMLGIANNLK 263 +S V++ EE + Q+ Y N++ +QT++ + N++ Sbjct: 503 QQSISGVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR 546 Score = 39.6 bits (92), Expect = 1e-05 Identities = 19/79 (24%), Positives = 34/79 (43%), Gaps = 14/79 (17%) Query: 5 LWTAASGMTAQQYNVDTIANNLSNVNTTGFKKIRAEFEDLIYQTHNRAGTPATENTLRPL 64 + A SG+ A Q ++T +NN+S+ N G+ + A N+ Sbjct: 4 INNAMSGLNAAQAALNTASNNISSYNVAGYTRQTT--------------IMAQANSTLGA 49 Query: 65 GNQVGHGTKIAATQRIFEQ 83 G VG+G ++ QR ++ Sbjct: 50 GGWVGNGVYVSGVQREYDA 68
ORFs having significant similarity with Known Virulence factors
LocusTag	Hits	Score	E-value	Comments
BBUZS7_RS05275	FLGHOOKAP1	43	9e-07	Flagellar hook-associated protein signature.
>FLGHOOKAP1#Flagellar hook-associated protein signature. Length = 546 Score = 43.0 bits (101), Expect = 9e-07 Identities = 14/64 (21%), Positives = 28/64 (43%) Query: 214 DTKTSGKAQEIDISLRPKIETETLEASNVNAVKEMVLMIEINRAYEANQKTIQTEDSLLG 273 T T + ++ ++ + S VN +E + + Y AN + +QT +++ Sbjct: 481 KTATLKTSSATQGNVVTQLSNQQQSISGVNLDEEYGNLQRFQQYYLANAQVLQTANAIFD 540 Query: 274 KLIN 277 LIN Sbjct: 541 ALIN 544 Score = 38.4 bits (89), Expect = 2e-05 Identities = 13/39 (33%), Positives = 23/39 (58%) Query: 4 GIYTAASGMMAERRKLDTVSNNLANIDLIGYKKDLSIQK 42 I A SG+ A + L+T SNN+++ ++ GY + +I Sbjct: 3 LINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMA 41