Release notes:
SSAKE 3.2 adjusts contig ends to find new extension possibilities. A bug that prevented SSAKE from exploring the entire read space for contig extensions seeded by shorter reads has been FIXED.
The new option (-t) will allow contig trimming in 3', one base at a time until a maximum base trim value (-t) is reached. This option yields longer contigs, but increases assembly run time and, at high -t values, might introduce contig misassemblies if your other run parameters (i.e. -m, -o and -r) are not stringent enough. At -m 16 -o 3 -r 0.7, best results were obtained at -t 1. That's because it removes bases that cause premature breaks during the fragment assembly. If set, end-trimming kicks in only when all possibilities have been exhausted for a contig extension.
This release also fixes a major bug that prevented SSAKE from exploring the entire read space for contig extensions seeded by shorter reads.
Changelog:
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