[Next-Generation Sequencing Book]
  • [Photo] Dr. David Langenberger • July 8, 2015
    December 15-17, 2015
    iad Pc-Pool, Rosa-Luxemburg-Straße 23, Leipzig, Germany
    http://www.ecseq.com/workshops/workshop_2015-02.html

    SCOPE & TOPICS

    The purpose of this workshop is to get a deeper understanding of the use of bisulfite-treated DNA in order to analyze the epigenetic layer of DNA methylation. Advantages and disadvantages of the so-called 'bisulfite sequencing' and its implications on data analyses will be covered. The participants will be trained to understand bisulfite-treated NGS data, to detect potential problems/errors and finally to implement their own pipelines. After this course they will be able to analyze DNA methylation and create ready-to-publish graphics.

    By the end of this workshop the participants will:
    • Be familiar with the sequencing method of Illumina
    • Understand how bisulfite sequencing works
    • Be aware of the mapping problem of bisulfite-treated data
    • Understand how bisulfite-treated reads are mapped to a reference genome
    • Be familiar with common data formats and standards
    • Know relevant tools for data processing
    • Automate tasks with shell scripting to create reusable data pipelines
    • Perform basic analyses (call methylated regions, perform basic downstream analyses)
    • Plot and visualize results (ready-to-publish)
    • Be able to reuse all analyses

    TARGET AUDIENCE

    • Biologists or data analysts with no or little experience in analyzing bisulfite sequencing data

    REQUIREMENTS

    • Basic understanding of molecular biology (DNA, RNA, gene expression, PCR, ...)
    • The data analysis will partly take place on the linux command line.

    IMPORTANT DATES

    Opening Date of Registration: June 1, 2015
    Closing Date of Registration: November 15, 2015
    Workshop: December 15-17, 2015 (8 am - 5 pm)

    Please visit the website for more information.

Discussion forums: Education: Epigenetics Workshop: DNA Methylation Data Analysis: How to use bisulfite-treated sequencing to study DNA methylation

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