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BACKGROUND

The Sanger Mouse Pipelines Genome Engineering group(GE) generates custom mutations in mice by gene targeting in embryonic stem (ES) cells and directly in the mouse zygote by applying cutting edge gene targeting technology.GE creates mutant alleles of interest to Sanger Institute Faculty, many of which are used as models for human disease. Our efforts benefit from extensive targeting vector/ES cell modular resources generated previously as part of the KOMP, EUCOMM, and EUCOMMTOOLs high throughput gene targeting programs and associated expertise as well as the successful implementation of CRISPR/Cas9 methods.

RESPONSIBILITIES

We are seeking a highly motivated and flexible individual to join the GE team as an Research Assistant or Advanced Research Assistant. The post holder's main responsibilities will be execution of day-to-day workflows of projects for the generation of targeting vectors, CRISPR/Cas9 reagents and the generation and validation of quality assured targeted mouse ES cells. Key responsibilities include execution and timely delivery of these resources, accurate record keeping, project troubleshooting and an ability to work across multiple projects. In addition, the group also has a research and development (R&D) commitment to implement new approaches that facilitate the insertion of complex alleles directly into the mouse zygote.

Previous experience of mammalian cell culture, especially mouse ES cell (mESC) culture is a prerequisite for this role. The candidate would also be expected to be able to genotype the mESC clones generated in order to provide quality assured reagents for microinjection. The candidate should have a broad knowledge of molecular biology techniques such as nucleic acid extraction, construct generation (via conventional cloning, Gibson assembly and/or recombineering), PCR, restriction enzyme analysis and sequencing. An understanding of conventional gene targeting and the more recent CRISPR/Cas9 genome engineering techniques would be advantageous. The successful candidate must be able to work as part of a team and also have the ability to independently handle complex workflows from project start to delivery. Good communication/record keeping skills are a key part of the role.

Some weekend cell culture is anticipated in this role.

REQUIREMENTS

Research Assistant Essential Skills:

Practical experience in cell culture
An understanding and practical experience in molecular biology strategies and techniques, including molecular cloning, nucleic acid isolation and purification, and PCR.
Ability to test and implement new approaches and techniques including troubleshooting
Capable of performing/tracking a variety of challenging projects simultaneously
Ability to work as part of a team and also independently once appropriate training and guidance have been provided.
Good communication skills to allow accurate information to be exchanged
Science degree in biological sciences
Practical laboratory experience
Ability to schedule and deliver simultaneous projects within agreed deadlines

Advanced Research Assistant Essential Skills:

Experienced in mammalian cell culture, especially routine mouse ES cells culturing.
An understanding and practical experience in molecular biology strategies and techniques, including some of the following: molecular cloning, construct generation (via conventional cloning, Gibson assembly and recombineering), PCR, restriction enzyme analysis and sequencing.
Isolation and purification of nucleic acid, particularly RNA based methodologies for the generation of CRISPR reagents.
Practical experience and theoretical understanding of genotyping techniques, including qPCR, long range PCR and sequencing and data interpretation there of.
Capable of performing/tracking a variety of challenging projects simultaneously
Ability to work as part of a team and also independently once appropriate training and guidance has been provided.
Good communication skills to allow accurate information to be exchanged
Science degree in biological sciences or extensive, proven practical experience
Ability to schedule and deliver simultaneous projects within agreed deadlines
Good computer skills

PREFERENCES

Experience of transfection, nucleofection and electroporation technologies would be advantageous
Practical experience in understanding gene structures on standard genome browsers
Practical experience and theoretical understanding of gene targeting strategies
Ability to test, implement and troubleshoot new approaches and techniques

TERMS

Permanent

LOCALE

The Institute is located near Cambridge, UK on the stunning Wellcome Genome Campus.

COMPENSATION

Salary in the region of £19,000 to £30,000 per annum depending on role and experience

HOW TO APPLY

For further information, job description and to apply; https://jobs.sanger.ac.uk/wd/plsql/wd_portal.show_job?p_web_site_id=1764&p_web_page_id=328434

DEADLINE

31st October 2017

POLICY

Wellcome Trust Sanger Institute welcomes applications from all candidates irrespective of age, disability, gender, gender identity, sexual orientation, race, religion or belief, or marital or civil partnership status.

Discussion forums: Opportunity: Research Assistant/Advanced Research Assistant @ Wellcome Trust Sanger Institute -- Hinxton/Cambridge, UK

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