BACKGROUND

Bioinformatics (Computational Biology) is a must skill required in every modern biomedical research lab. Installing and configuring a wide variety of computational biology tools is a cumbersome task that requires software engineering skills. This hands-on training course will introduce participants to a custom, all-in-one, fully loaded linux desktop (with windows like graphical user interface) machine, that comes with hundreds of popular computational biology (bioinformatics) tools required for a successful modern biomedical research lab.

OBJECTIVES

The participants will be provided with end-to-end hands-on training, along with introduction tobasic concepts, in using popular tools and techniques for sequence analysis, structure analysis, function prediction, biological database searching, "omics" data analysis, pathway analysis, datavisualization, data curation and integration, linux, R, perl and scripting basics.rticipants should be comfortable with basic computer skills.

Hands-on Skills/Tools Taught:

Databases: NCBI-ENTREZ, UniProt, PDB, STRING, Others.
Sequence analysis and function predictions: EMBOSS suite & others
Local Alignment: EMBOSS-WATER
Global Alignment: EMBOSS-NEEDLE
Similarity search: NCBI BLAST, PSI-BLAST
Multiple sequence alignment: Clustal Omega, MUSCLE, MAFFT
Phylogenetics: MrBayes, MEGA, FigTree and Dendroscope
Motif finding, analysis: MEME suite
Structure prediction, visualization & analysis: PyMOL, Chimera, iTASSER
Transcriptome analysis: NCBI GEO, Tuxedo tools, R
Enrichment analysis: DAVID
Pathway analysis: Cytoscape
Programming: R, Perl, Python
Platforms: EMBOSS, UGENE, H2O, Galaxy