Not logged in

Bioinformatics.org

Membership (44431+)

Basic Free!
Professional

Group hosting

Hosted groups (569)

Wiki
Franklin Award
Sponsorships

Careers

About bioinformatics
Bioinformatics jobs

Research

All information groups
Online databases

Online analysis tools

Online education tools

Atlas of Macromolecules

More tools

Development

All software groups
FTP repository
SVN & CVS repositories
Mailing lists

Forums

News & Commentary

Submit

OVERVIEW

In recent years single-cell RNA-seq (scRNA-seq) has become widely used for transcriptome analysis in many areas of biology. In contrast to bulk RNA-seq, scRNA-seq provides quantitative measurements of the expression of every gene in a single cell. However, to analyze scRNA-seq data, novel methods are required and some of the underlying assumptions for the methods developed for bulk RNA-seq experiments are no longer valid. In this course we will cover all steps of the scRNA-seq processing, starting from the raw reads coming off the sequencer. The course includes common analysis strategies, using state-of-the-art methods and we also discuss the central biological questions that can be addressed using scRNA-seq.

FORMAT

The course will be delivered over the course of five days. Each day will include a lecture and laboratory component. The lecture will introduce the topics of discussion and the laboratory sessions will be focused on practical hands-on analysis of scRNA-seq data. These sessions will involve a combination of both mirroring exercises with the instructor to demonstrate a skill as well as applying these skills on your own to complete individual exercises. After and during each exercise, interpretation of results will be discussed as a group. Computing will be done using a combination of tools installed on the attendees laptop computer and web resources accessed via web browser.

TARGETED AUDIENCE & ASSUMED BACKGROUND

This course is aimed at researchers and technical workers who are or will be analyzing scRNA-seq data. The material is suitable both for experimentalists who want to learn more about data-analysis as well as computational biologists who want to learn about scRNASeq methods. Examples demonstrated in this course can be applied to any experimental protocol or biological system.

REQUIREMENTS

Working knowledge of unix (managing files, running programs)
Programming experience in R (writing a function, basic I/O operations, variable types, using packages)
Bioconductor experience is a plus.
Familiarity with NGS data and its analyses (using alignment and quantification tools for bulk sequencing data)

INSTRUCTORS

Dr. Ayshwarya Subramanian (Harvard School of Public Health, Broad Institute, Dana-Farber Cancer Institute, US)
Dr. Dana Silverbush (Harvard School of Public Health, Broad Institute, Dana-Farber Cancer Institute, US)
Dr. Ehsan Habibi (Harvard School of Public Health, Broad Institute, Dana-Farber Cancer Institute, US)

Discussion forums: Education: Course on the analysis of single cell RNA-seq data

Expanded view | Monitor forum | Save place

Start a new thread:

You have to be logged in to post a reply.