[Next-Generation Sequencing Book]
  • [Photo] Vijayaraj Nagarajan • August 24, 2018
    September 25-28, 2018
    National Institutes of Health
    9000 Rockville Pike
    Building 60, Room 162
    Bethesda, MD 20892, USA
    https://faes.org/events/biotech-74-advanced-transcriptomics-rna-seq-analysis-4-day-hands-workshop-3

    OBJECTIVES

    Apart from performing the routine differential expression analysis using two different suite of tools, this hands-on training will help participants learn advanced RNA-Seq analysis techniques and tools for detecting snps, fusion genes, allele specific expressions, circular RNAs, viral/bacterial sequence identification, alternative polyadenylation and transcriptional regulatory network analysis.

    HIGHLIGHTS

    • Participants will use a Graphic User Interface based Linux Desktop environment, specially configured for advanced RNA-Seq analysis in the Amazon Cloud
    • Training provided by experienced active NIH researchers
    • Cookbook style bound manual for all exercises
    • Direct, after training support through exclusive forum membership
    • Continuing Education Credits

    HANDS-ON SKILLS & TOOLS TAUGHT

    • Differential expression analysis: New Tuxedo tools
    • Differential expression analysis: Trinity tools
    • Fusion gene analysis : STAR-Fusion
    • SNP detection : GATK
    • Allele specific expression : WASP
    • Circular RNA identification : circexplorer
    • Alternative polyadenylation : DaPars
    • Viral integration : VirusFinder
    • Network analysis : Cytoscape

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