Resume for Sabeen Raza - Bioinformatics.org

SRaza • February 4, 2002
Resume for Sabeen Raza
(1 comment)
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Sabeen Raza
E-Mail: S1MR4N[at]aol.com

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OBJECTIVE
Having been introduced to biological research in the third year of my degree and Bioinformatics at Zeneca, I intend to obtain a long-term career in Bioinformatics.

EDUCATION
Oct 2000 - Sept 2002
Birkbeck College, University of London,
MSc. in Molecular Modelling and Bioinformatics
Project Supervisor: Dr Sylvia Nagl
Title of project: Computational search protocol for the characterisation of proteins.

Sept 1993 - July 1997
University of East London,
BSc. (Hons) in Applied Biology
Project Supervisor: Dr David Rowley
Title of project: Comparing the number of hydrogen peroxide producing and non producing Lactobacilli from HVS of women suffering from bacterial vaginosis.

EMPLOYMENT HISTORY

July 1998 - Oct 1999
Department: INVENTIONS; Syngenta (previously known as Zeneca Agrochemicals).
Molecular biologist and a Quality Assurance officer in the Plant Biotechnology Section of the. Duties included the verification of the integrity of clones being produced for transformation into plants using Molecular Biological methods and various Bioinformatics packages. The information was stored on a self-written and maintained fully searchable MS Access database.

Feb 1998 - July 1998
Department of Molecular Genetics at the Institute of Ophthalmology.
Unpaid work as a research assistant for a Scientific Officer. The project involved working on a corneal dystrophy called Congenital Hereditary Endothelial Dystrophy (CHED), that had previously been mapped to a region on chromosome 20p11.2. Duties also included the physical mapping of the disease locus.

Sept 1995 - Aug 1996
Joint Microbiology Research Unit; King's College School of Medicine and Dentistry.
Routine work involved the taxonomy of the two main genospecies of A. naeslundii by Microbial and Molecular Biological methods. Main Responsibilities included isolating single colonies of A naeslundii, isolating the chromosomal DNA, running RFLP analysis on the restriction digests to detect the differences in the RFLP patterns of the different strains within the same genospecies. Further identification of the different strains within the same genospecies was carried out by PCR.

COMPUTER SKILLS
· Operating Systems
Windows/DOS, Windows NT, Unix/Linux

· Programming Languages
C++, Java, Perl-CGI, Java Script (DHTML)

· Markup Languages
HTML, XML

· Modelling Software
AMBER, GROMEX, Rasmol/Chime, Sybyl/O, Quanta/CHAARM

· Databases
SQL, MySQL, MS Access

· Bioinformatics Packages
Vector Nti suite, Sequencer, Map Draw, Enhance, Meg Align,Primer Select, Macaw, Seqman, Macvector, Geneworks

· Search Tools
Gopher, MedLine, BIDS, SRS, Zsearch, BLAST(n,p&x), Expasy(SWISS-PROT &TREMBL), Interpro, Pfam

LABORATORY SKILLS
· Expertise in chromosomal and plasmid DNA isolation from bacteria (Mini, Midi and Maxi preps) and Human genomic DNA from peripheral blood samples;
· DNA quantification using various methods; using Hoescht Dye method and Spectrophotometry
· Positional cloning techniques, involving the isolation of YACs from YAC libraries
· Pulse Field Gel Electrophoresis (PFGE)
· Polymerase Chain Reaction (PCR)
· STS content mapping by PCR
· Competent in RFLP analysis
· Northern and Southern blotting
· Expertise in agarose gel electrophoresis
· Competent in Column chromatography
· Confocal microscopy
· Some experience with various Immunological techniques such as ELISA and RIA
· Non radioactive sequencing using ABI technology

REFERENCES
1. Professor Julia M. Goodfellow, Head of School, School of Crystallography, Birkbeck College, University of London, Malet Street, London, WC1E 7HX. Tel: +44 (0)20 7631 6800. E-mail: J.Goodfellow[at]mail.cryst.bbk.ac.uk

2. Dr Sylvia Nagl, Department of Biochemistry, University College London (UCL), Gower Street, London, Tel: +44 207 679 7034. E-Mail: nagl[at]biochemistry.ucl.ac.uk

3. Sarah Matthews, Syngenta (Agrochemicals), Jealotts Hill, Bracknell, Birkshire, RG42 6ET. Tel: +44 1344 414 99. E-Mail: Sarah.Matthews[at]Syngenta.com

4. Mr. Neil Ebeneezer, University College London, Research assistant, Institute of Ophthalmology, 11-43 Bath Street, London EC1 V 9 EL. Tel +44 171 608 6915. E-mail smgxnde[at]ucl.ac.uk

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