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gCut is a tool for restriction analysis in a DNA sequence. It will find the restriction sites of a given enzyme (tetra and hexa enzymes only) and perform the cut. It's written in Python, compatible with FASTA format sequences, and can analyse sequences of any size.
Example of tetra and hexa enzymes: EcoRI G/AATTC (hexa), HaeIII GG/CC (tetra)AVAILABILITY
http://gcut.sourceforge.net
Discussion forums: Software: gCut 1.0 DNA restriction analysis tool
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