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Hirendra Vikram
e-mail: hirendrav@yahoo.com
URL: http://exon.tn.nic.in/~vikram
Career Plans and Objectives:
Want to pursue research, in the field of Genomics/Bioinformatics,
towards PhD degree. I will like to actively participate in conferences /
symposiums / poster presentations relating to my work and will love
to be engaged actively in other scientific activities.
Present Position:
Presently working as Research Assistant in Bioinformatics with
Prof. S. Krishnaswamy, Professor in Bioinformatics, Madurai Kamaraj
University, Madurai.
Work involves Application of Information Theory on Genomic
Sequences.
Educational Qualifications:
Certificate in Computing - Indira Gandhi National Open University
Final Result: 79.3%
Period
of
Study
Programme
University/
Board
%
Obtained
Subjects Studied
2001-
2002
Advanced
Diploma in
Bioinformatics
Madurai
Kamaraj
University
86.3%
(Ist Rank)
Genomics and Proteomics, Structural Biology,
Molecular simulations, Computer and Internet
Programming, Basic Bioinformatics,
Commercial applications, + Industrial training
+ Diploma Project
1999-
2001
Master of
Science
Biotechnology
Aligarh
Muslim
University
62.5%
Biochemistry, Microbiology, Cell and Mol Bio,
Immunology, Enzymology, Biotechnological
Techniques, Chemistry, Mathematics,
Bioinformatics, Computers, + Project +
Seminar lectures
1996-
1999
Bachelor of
Science
Zoology
(Honors)
Delhi
University
61.3%
Zoology (Chordata, Non-Chordata, Physiology,
Biochemistry, Ecology, Entomology), Botany,
Chemistry
1996
All India Senior
School
Certificate
Exam
Central
Board of
Secondary
Education
69.8%
Physics, Chemistry, Biology, Mathematics,
English
1994
All India
Secondary
School Exam
Central
Board of
Secondary
Education
77.2%
Science, Social-Science, English, Mathematics,
Hindi
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Awards and Fellowships:
Ø CSIR NET July.2001 Life Sciences Qualified: JRF (CSIR)
was among first 20% All India
Ø CSIR NET Dec.2000 Life Sciences Qualified: lecturership
Ø GATE 2001 Life Sciences Score: 90.93
Ø ICMR 2001 Life Sciences Qualified:JRF
Rank: 21
Ø Bioinformatics Studentship Dept. of Biotechnology 2001-2002
(Govt. of India)
Ø First Rank Holder in Advanced Diploma in Bioinformatics at Madurai
Kamaraj University
* C S I R (Council of Scientific and Industrial Research) ? N E T is a nation-wide
exam for granting research fellowships.
* G A T E (Graduate Aptitude Test in Engineering) ? conducted by Indian Institute
of Technology.
* I C M R (Indian Council of Medical Research)- J R F is also a Nation-wide exam for
granting research fellowships.
Project work and dissertations: (Summary attached)
2002 : Dissertation work ? Whole Genome Comparative Genomics:
Information Theory & Evolution?.
2001 : Dissertation work was performed on the project entitled
?Purification of Cajanus cajan root lectin and its interaction with
rhizobial lipopolysaccharide?
2000 : Summer training was done at Biosensor lab, Centre of
Biochemical technology, Delhi. The work entitled ? Development of
Biostrips for detection of Calcium and Alkaline Phosphatase?.
Industrial Experience and skills:
Worked in Molecular Connections Pvt. Ltd, a Dr.Reddy?s lab concern,
in Bangalore on an ongoing project on Database curation, for a period
of one and a half months.
Bioinformatics:
Computational Analysis of DNA & Protein Sequence, Genome
Analysis, Usage of public databases, Usage of search engines,
services and software in Bioinformatics, Usage of GCG Wisconsin
package, EMBOSS, Molecular Simulations, INSIGHT II, Amber 4.
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Computers:
Ø Operating systems: Unix, Linux, MS-DOS, Windows 2000, NT
Ø Programming languages: C, C++ and Visual Basic
Ø Internet Markup languages: HTML, DHTML, and XML
Ø Scripting Languages: Perl, Python
Ø Database Applications: SQL -Server
Biotechnological Techniques:
Biosensor Development, Column chromatography, Protein purification
techniques, Plasmid isolation, Protein/carbohydrate/DNA estimation,
Lectin haemagglutination assays, ELISA, PAGE, SDS PAGE, Circular
Dichroism, UV-Visual spectroscopy
Publications:
Purification of Cajanus Cajan root lectin and its interaction with
rhizobial lipopolysaccharide as studied by different spectroscopic
techniques.
Arch Biochem Biophys. 2001 Dec 1; 396(1): 99-105.
PMID: 11716467
?Arsenazo III test strip for rapid detection of hardness of water?,
Indian Journal of Chemical Technology, Vol.8, July 2001.pp.252-254
?Disposable Biostrips for detection of Alkaline Phosphatase in
Pasteurized Milk?, abstract accepted by DRDO.
Personal Information:
Age : 23
Sex : Male
Date of Birth : 26th December 1978
Nationality : Indian
Marital Status : Single
Languages Known : English, Hindi, Learning German
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Referees:
(1) Prof. M.Saleemuddin
Co-coordinator,
Interdisciplinary Biotechnology Unit
Aligarh Muslim University,
Aligarh-PIN- 202001,
Uttar Pradesh, India.
Email: alg_btisamua@sancharnet.in
(2) Prof. S.Krishnaswamy
Bioinformatics Centre,
Madurai Kamaraj University,
Palkalai Nagar,
Madurai-PIN-625021
Tamil Nadu, India
e-mail: Krishna@mrna.tn.nic.in
mkubic9@giasmd01.vsnl.net.in
Phone:91-452-459141 (office), 91-452-458638 (res.)
Fax: 91-452-859105
(3) Dr. Rizwan Hasan Khan
Interdisciplinary Biotechnology Unit
Aligarh Muslim University,
Aligarh-PIN- 202001,
Uttar Pradesh, India.
Phones: 0571-701081(Office)
0571-403457(Res)
Fax: 91-571-701081
Email: rizwanhkhan@hotmail.com
Address for Hirendra Vikram
Communication C/o Prof. S. Krishnaswamy,
Bioinformatics Centre,
School of Biotechnology,
Madurai Kamaraj University,
Palkalai Nagar, Madurai-625021.
Tamil Nadu, INDIA
Permanent Address: 26, Patel Apartments,
Plot-10, Sector-13, Rohini,
Delhi-110085.
Phone-91-011-7568138, 91-011-7567062.
E-mail: hirendrav@yahoo.com
URL: http://exon.tn.nic.in/~vikram
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Research Experience: Projects Summary
Ø Whole Genome Comparative Genomics: Information
Theory and Evolution ( March- April 2002 )
Supervisor: Prof. S. Krishnaswamy
Bioinformatics Centre,
Madurai Kamaraj University,
Madurai, Tamil Nadu, INDIA
Summary: Information Theory was applied to whole genomic
sequences of available 74 Prokaryotes, 92 Chromosomes from 10
Eukaryotic organisms, 69 mitochondrial genomes from wide variety of
genomes. Information theoretic parameters were calculated for each
of the genomes and graphical displays were prepared to analyze the
observations.
Among interesting findings, we were able to locate the possible
ancestor for mitochondria. We were able to observe the relationships
between genomes of prokaryote and eukaryotes within a given
subgroup or among different groups.
Additionally, I collected most of the original reference papers
for published genome sequences, and have a personal repository.
The Skill set used in the project involved programming skills
in language C and Perl, use of sequence analysis package GCG and
EMBOSS, use of Public Genomic Databases and techniques to retrieve
and store the sequences. The environment of work was Linux/Unix
and Windows NT.
NOTE: Manuscript is under preparation for publication in a reputed
Journal.
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Ø To undergo Industrial training and participate in an
ongoing project on Creation of a database for Protein-
Protein interactions. (February ? March 2002)
Supervisor: Prof. S. C. Nair
Molecular Connections Pvt. Ltd.
Bangalore, India.
Summary: Swiss Prot entries for nuclear hormone receptors were
taken and for each protein, various databases for interacting proteins
were checked. PubMed was also searched and abstracts were read
carefully so as to find a clue about a possible interaction in available
literature. The data was fed into XML forms in specified fields with
proper validations.
Search was also carried out for finding natural mutants for a
given protein.
The skill set involved was the extensive knowledge of XML,
the use of available public databases for Interacting proteins like DIP
and BIND, PMD for searching mutants. Use of NCBI?s PubMed was
done extensively.
NOTE: This was a collective teamwork and it resulted in a product
from company, which can be accessed by creating an account with
the company.
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Ø Purification of Cajanus Cajan root lectin and its
Interaction with Rhizobial Lipopolysaccharide.( 2000-
2001 )
Supervisor: Dr. Rizwan Hasan Khan
Interdisciplinary Biotechnology Unit,
Aligarh Muslim University,
Aligarh.
Summary: The Pigeon Pea seed were grown on soil as well as on
0.8% agar media. The Pigeon Pea Root Lectin (PPRL) was isolated
from different days seedlings. Different days root homogenate
preparation showed positive hemagglutination activity of root
homogenate was found to be maximum in 7 days old preparation
grown on agar medium. Hemagglutination inhibition shows that root
lectin was glucose/mannose specific. These results were further
confirmed by lectin-IgM turbidity method. Further, 7 day old root
homogenate was purified on Mannose Agarose column and used for
advance studies.
The affinity purified root lectin showed similar relative mobility
on SDS-PAGE as that of seed lectin, indicating the presence of two
subunits. Studies were also performed on the interaction of rhizobial
lipopolysaccharide with Pigeon Pea Root Lectin by spectrophotometric
fluorescence and circular Dichroism.
Inhibition studies by sugars were also performed on the
rhizobial lipopolysaccharide and Pigeon Pea Root Lectin complex.
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These studies have shown that bacterial LPS- Lectin interaction is
specific.
For this project, I also worked in Lectin Research Lab, Center
for Biochemical Technology, Delhi.
The nature of work was mainly Biochemical, Microbiological and
spectrometric and the skills developed were of mainly protein
purification, LPS isolation, Bacterial culture, fluorescence, Circular
Dichroism.
NOTE: This work yielded in publication in Archives of Biochemistry
and Biophysics. A copy is attached.
Ø Development of Biostrips for detection of Calcium and
Alkaline Phosphatase. ( May ? July 2000 )
Supervisor: Dr. Ashok Kumar
Biosensor Lab
Centre for Biochemical Technology
Summary: The work involved the development and optimization of
visually available dry-reagent test strip technique for detection of
Alkaline Phosphatase in pasteurized milk [A] and qualitative and
semi-quantitative estimation of hardness of water [B]
In dry reagent strip [A], immobilized p-Nitrophenyl phosphate
reacts with Alkaline Phosphatase present in the milk to form p-nitro
phenol, which reacts with dye to give a colored complex.
The blue color of strip changes into shades of green after 2
seconds of dipping; the changed color can be compared with the color
chart prepared. The efficiency was checked by using standard
solution of Alkaline Phosphatase in triple distilled water.
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In dry-reagent strip [B], immobilized Arsenazo dye reacts with
calcium present in water to form calcium Arsenazo complex that
changes the color of the strip. The evaluation was done after 15
seconds to visualize a change in the color and then compared with
the color chart printed on the bottle. The efficiency of dry-reagent
strip was carried out using the standard solution of calcium chloride
or bicarbonate in triple distilled water and comparing the values
obtained by various other reported methods.
The strips are stable at room temperature for several months if
stored under humidity free conditions. Such type of strip is useful for
qualitative as well as semi-quantitative estimation of hardness of
water to be used in industrial applications and for daily requirement.
The strip can also be used for rapid geological survey of water. Being
a visual assessment technique, it does not require any specialized
training or sophisticated equipments. This type of technique is very
simple, economical and can be used in remote areas of country where
sophisticated instruments or techniques are not available.
NOTE: This work yielded in publication in Indian Journal of Chemical
Technology . A copy is attached.
Ø WILD WORLD
Supervisor: Dr. Lakshmi Narula
Reader, Zoology Department,
S.G.T.B. Khalsa College
Delhi University, Delhi
Collected information available Wild animals available in our country.
Newspaper articles and references about atrocities committed on
them were displayed. National Zoological park was visited and
instead of shooting them with a camera, sketches of them were made
and submitted.
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Scientific Activities
Workshops Attended
27 July ? 28 July 2002 Attended National Children?s Science Congress
(NCSC- 2002) , State Level Resource Persons? Workshop organized by Tamil Nadu
Science Forum (TNSF) , Supported by NCSTC-DST, Govt. of India, New Delhi.
25 June - 29 June 2002 Workshop on theoretical biology concepts on E-cell,
V-cell and genesis, conducted by MatScience, Chennai, India.
2002 Participated in Bioinformatics Workshop conducted by Bioinformatics
Center, Madurai Kamaraj University, India.
2001 Attended National Symposium on Stability and Stabilization of
Biomolecules, organized by Interdisciplinary Biotechnology Unit, Aligarh Muslim
University, Aligarh, India.
2000 Attended seminar and lectures on Plant Biotechnology in 2nd
Biotechnology International conference, conducted by Exhibition India at
National Science Center, Pragati Maidan, New Delhi as a visitor audience.
1998 Attended workshop on Energy and Environment Awareness conducted
by Delhi University Energy and Environment Group
Awards Received ( Related to Science)
2001 Got 1st Prize in Model Presentation, organized on Science Day by
Interdisciplinary Biotechnology Unit, AMU, sponsored by DBT, for making model on
DNA FINGERPRINTING.
2000 Got 2nd Prize in poster/ Model Presentation, organized on Science Day by
Interdisciplinary Biotechnology Unit, Aligarh Muslim University, for making model
on ? Beyond 5000: Human Engineering Project?, a Science Fiction
1999 Got 3rd Prize in Poster making competition, organized by GENESIS, for
making a poster on future of biotechnology.
1999 Got 1st Prize in Poster Making competition, organized by ZOOLGICAL
SOCIETY, SGTB Khalsa College, for making 8ft Computerized statue of Liberty
concerning DNA Finger Printing.
1998 Got 3rd Prize in Cartoon Making competition, organized by VIGYAN
VICHITRA, for making a cartoon on genetic engineering.
Extracurricular Activities
1998 - Have organized a local tour of students to National Science Centre, New
Delhi and Explained the experimental setups and exhibits there.
1997-1998 Organized Science Quiz Competitions for School Students.
1995?1997 Organised Poster making and drawing competitions for children.
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