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Basic Cell Culture
Submitted by Qinghua Zhou; posted on Thursday, April 11, 2002 (2 comments)
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Hello everyone,
I¡¯d like to ask two questions:
1. I want to isolate osteoblast from mice, I don¡¯t know which part is better, from long bone, OK? If I use collagenase P to digest bone to remove fibroblast contamination, do you know the appropriate concentration of collagenase P and its digestion time and temperature?
2. When I want to culture epidermal keratinocytes using Sigma Epilife serum-free medium, do I need to coat the dish with collagen, if need, what kind of collagen is OK?
Thanks!
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Hello everyone,
I¡¯d like to ask two questions:
1. I want to isolate osteoblast from mice, I don¡¯t know which part is better, from long bone, OK? If I use collagenase P to digest bone to remove fibroblast contamination, do you know the appropriate concentration of collagenase P and its digestion time and temperature?
2. When I want to culture epidermal keratinocytes using Sigma Epilife serum-free medium, do I need to coat the dish with collagen, if need, what kind of collagen is OK?
Thanks!
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