Can anyone help me with the answers for these two questions? 1. What does the 'rare codons' means if it is in a predicted ORF? 2. We were searching for nucleic acid subsequences in the Enterobacter T7 ligase gene. We found 380 subsequences and 5 Ribosome Binding Site (RBS) in this gene. All 5 RBS were in the ORF. So the question is does any of the RBS sites make sense compared to the ORF analysis? Thanks for any help. Tom