Bioinformatics.org
Not logged in
  • Log in
  • Bioinformatics.org
    Membership (42689+) Group hosting [?] Wiki
    Franklin Award
    Sponsorships

    Careers
    About bioinformatics
    Bioinformatics training
    Bioinformatics jobs

    Research
    All information groups
    Online databases Online analysis tools Online education tools More tools

    Development
    All software groups
    FTP repository
    SVN & CVS repositories [?]
    Mailing lists

    Forums
    News & Commentary
  • Submit
  • Archives
  • Subscribe

  • Jobs Forum
    (Career Center)
  • Submit
  • Archives
  • Subscribe
  • News & Commentary - Message forums

    Software: IPC 2.0: prediction of isoelectric point and pKa dissociation constants
    Submitted by Lukasz Pawel Kozlowski; posted on Thursday, July 08, 2021

    Submitter

    IPC 2.0 – Isoelectric Point Calculator 2.0 is a web service and a standalone program for the estimation of protein and peptide isoelectric point (pI) and dissociation constant (pKa) values using a mixture of deep learning and support vector regression models.

    Input: amino acid sequence(s)
    Output: pI values predicted by >15 methods alongside with pKa dissociation constants for charged residues

    Isoelectric point, the pH at which a particular molecule carries no net electrical charge, is a critical parameter for many analytical biochemistry and proteomics techniques, especially for 2D gel electrophoresis (2D-PAGE), capillary isoelectric focusing (cIEF), X-ray crystallography, and liquid chromatography--mass spectrometry (LC-MS).

    According to the benchmarks, the prediction accuracy (RMSD) of IPC 2.0 for proteins and peptides outperforms previous algorithms: 0.848 versus 0.868 and 0.222 versus 0.405, respectively. Moreover, the IPC 2.0 prediction of pKa using sequence information alone was better than the prediction from structure-based methods (0.576 versus 0.826) and a few folds faster.

    AVAILABILITY

    IPC 2.0 is available at www.ipc2-isoelectric-point.org

    REFERENCE

    Kozlowski LP (2021) IPC 2.0: prediction of isoelectric point and pKa dissociation constants. Nucleic Acid Res. DOI: doi.org/10.1093/nar/gkab295

    Expanded view | Monitor forum | Save place

    Start a new thread:
    You have to be logged in to post a reply.

     

    Copyright © 2021 Scilico, LLC · Privacy Policy