The estimation of the isoelectric point (pI) based on the amino acid sequence is useful for many biochemistry and proteomics techniques such as 2D-PAGE, IEF or mass spectrometry.

Isoelectric Point Calculator allows for prediction of pI using different algorithms (17 algorithms implemented including two new ones).

IPC outperform previous algorithms by at least 14.9% for proteins and 0.9% for peptides (on average, 22.1% and 59.6%, respectively), which corresponds to an average error of the pI estimation equal to 0.87 and 0.25 pH units for proteins and peptides, respectively.

AVAILABILITY

The IPC can be accessed freely at http://isoelectric.ovh.org as a web service or standalone program.

ARTICLE

doi: http://dx.doi.org/10.1101/049841