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The estimation of the isoelectric point (pI) based on the amino acid sequence is useful for many biochemistry and proteomics techniques such as 2D-PAGE, IEF or mass spectrometry.
Isoelectric Point Calculator allows for prediction of pI using different algorithms (17 algorithms implemented including two new ones).
IPC outperform previous algorithms by at least 14.9% for proteins and 0.9% for peptides (on average, 22.1% and 59.6%, respectively), which corresponds to an average error of the pI estimation equal to 0.87 and 0.25 pH units for proteins and peptides, respectively.AVAILABILITY
The IPC can be accessed freely at http://isoelectric.ovh.org as a web service or standalone program.ARTICLE
doi: http://dx.doi.org/10.1101/049841
Discussion forums: Resources: IPC: Isoelectric Point Calculator
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Submitted by
Lukasz Pawel Kozlowski
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posted on
Thursday, May 27, 2021
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The webserver has been moved to isoelectric.org
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