SCOPE & TOPICS

• Be familiar with the sequencing method of Illumina
• Understand how bisulfite sequencing works
• Be aware of the mapping problem of bisulfite-treated data
• Understand how bisulfite-treated reads are mapped to a reference genome
• Be familiar with common data formats and standards
• Know relevant tools for data processing
• Automate tasks with shell scripting to create reusable data pipelines
• Perform basic analyses (call methylated regions, perform basic downstream analyses)
• Plot and visualize results (ready-to-publish)
• Be able to reuse all analyses

TARGET AUDIENCE

• Biologists or data analysts with no or little experience in analyzing bisulfite sequencing data

REQUIREMENTS

• Basic understanding of molecular biology (DNA, RNA, gene expression, PCR, ...)
• The data analysis will partly take place on the linux command line.

IMPORTANT DATES